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BioTepp, Inc. January 2011 VIROSOFT ® CP4 BioTepp, Inc

organic apple insect control program biological 7-16-14

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beetleGONE! is derived from a natural microbe called Bacillus thuringiensis subsp. galleriae. It is considered compliant with the USDA National organic program positive list. beetleGONE! fits well within an integrated pest management program IPM. It is complimentary to mating disruption, granulosis virus, other Bt strains active against Lepidoptera (moths), spray oil and other larvacides. It was developed in collaboration with Michigan State, U of Wisconsin, UC Davis, Joliet Community College and other organizations. It does not impact bees or other beneficial insects and may be applied while bees are foraging without worries.

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Page 1: organic apple insect control program biological 7-16-14

BioTepp, Inc. January 2011 VIROSOFT® CP4

BioTepp, Inc

Page 2: organic apple insect control program biological 7-16-14

VIROSOFT CP4 HOW IT WORKS NATURALLY

Virus occlusion bodies (OB) are viral DNA encoded proteins within a protective protein coat. After codling moth egg hatch OBs are eaten by neonate larvae.

infected larvae The virus infects the larvae, reproduces and destroys the apple/pear worm. This stops feeding and worms die within 3-7 days.

Photos courtesy of USDA ARS

Neonate

Page 3: organic apple insect control program biological 7-16-14

BioTepp, Inc

VIROSOFT® CP4

Unique “Quebec” CP4 virus strain for Codling Moth larvae population management & control

A naturally effective substitute for CM insecticides

Meets all orchard sustainable & organic standards

No effect on beneficial insects or other creatures

Page 4: organic apple insect control program biological 7-16-14

BioTepp, Inc

Managing Codling Moth with Granulovirus and Spinosad Dept. of Entomology, 2003

WSU Tree Fruit Research and Extension Center, Wenatchee, WA Kieth R. Granger, Jay F. Brunner and Michael D. Doerr

Treatment Rate AI/A Spray

1st

generation

(OBx1013) Interval (#aplc.) Moths/tree

Virosoft® 0.38 10 d (8)

0

Untreated --- ---

17.6

Virosoft is a registered trademark of BioTepp, Inc.,

Page 5: organic apple insect control program biological 7-16-14

BioTepp, Inc.

Comparison of Codling Moth Virus Products 2006

Michigan State University Trevor Nichols Research Complex

L. Gut, M. Hass, C. Hipshier, J. Wise & K. Schoenborn

Treatment Rate oz/Ac

1st generation

Interval (#aplc.) % shallow

stings % deep entries

Virosoft CP4 2 7 d (5) 11.2

11.2

Untreated --- --- 8.8

33.5

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BioTepp, Inc

VIROSOFT® CP4

OMRI Listed®

Treated fruit meets sustainable standards

Page 7: organic apple insect control program biological 7-16-14

BioTepp, Inc

VIROSOFT FORMULA IS STABLE J E C O N E N T O M O L . 2 0 0 8 A P R ; 1 0 1 ( 2 ) : 2 8 8 - 9 4 .

E F F E C T O F T E M P E R A T U R E O N L O N G - T E R M S T O R A G E O F C M G V F O R M U L A T I O N S . L A C E Y L A , H E A D R I C K H L , A R T H U R S S P .

Storage Stability Test Temperature

36° F 77° F 95° F

VIROSOFT CP4 31 mo.+ 31 mo.+ 9 mo.

Months with = to or > 95% CM larvae mortality at above temperatures

+ stability test stopped before end point reached

Will store even longer if refrigerated or frozen

Page 8: organic apple insect control program biological 7-16-14

BioTepp, Inc

VIROSOFT® CP4

Apply 2-3.2 oz/acre every 7-14 days

If Codling Moth pressure is high use 7 day interval

Begin sprays at egg hatch (2 weeks after petal fall) and continue as needed but apply at least 2 sprays per CM generation

Apply in enough water for thorough coverage of the tree bark, foliage and fruit

May be applied to Apple, Pear, Plum & Walnut trees

Page 9: organic apple insect control program biological 7-16-14

BioTepp, Inc

VIROSOFT® CP4 IN PROGRAMS

Apply Virosoft CP4 in IPM programs with dormant oil, mating disruption, other larvacides, kaolin clay and cultural controls

Optimize control of Codling Moth and other insects:

Page 10: organic apple insect control program biological 7-16-14

BioTepp, Inc

IPM & ORGANIC PROGRAM EXAMPLES AGAINST 1 ST GENERATION CM & LR

SOFT START ALLOWS BENEFICIALS TO ASSIST ASSUMES USE OF MATING DISRUPTION & SCOUTING

Timing Delayed Dormant

Petal Fall 50-100 Degree Days

100-200 Degree Days

1st Cover 220-250 Degree Days

Delayed 1st Cover 350 Degree Days

2nd Cover 450-600 Degree Days

Target CM eggs

LR larvae & CM eggs

CM eggs CM larvae CM larvae CM larvae

IPM 1 OP+0il

larvacide + oil

Virosoft® + larvacide

Virosoft + lavacide

IPM 2 OP+0il

ovacide/ larvacide

Virosoft + larvacide

Virosoft + larvacide

Organic Hort 0il

Entrust + oil

Virosoft + oil +kaolin

Virosoft + kaolin or Entrust®

Virosoft is a registered trademark of BioTepp, Inc. Entrust is registered trademark of Dow Agrosciences LLC

Page 11: organic apple insect control program biological 7-16-14

BioTepp, Inc

VIROSOFT CP4

Recommended reading:

Lacey, Lawrence A., Thomson, Donald, Vincent,

Charles and Arthurs, Steven P.(2008)'Codling moth

granulovirus: a comprehensive review',Biocontrol

Science and Technology,18:7,639 — 663

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BioTepp, Inc

THANK YOU

TEL. 916 719 6176

[email protected]

WWW.BIOTEPP.COM