Athar Hussain

Roll No.2015

BS(Hon)

Bioinformatics and Biotechnology

Govt.College University Faisalabad

Email: Atharmutahari@gmail.com

Construction of Evolutionary tree Using MEGA

MEGA, Molecular Evolutionary Genetics Analysis, is a

freely available software for conducting statistical analysis of

molecular evolution and for constructing phylogenetic trees.

MEGA is an integrated tool for conducting :

• Sequence alignment,

• Inferring phylogenetic trees,

• Estimating divergence times,

Introduction

Conti……..

• Estimating rates of molecular evolution,

• Inferring ancestral sequences,

• Testing evolutionary hypotheses.

MEGA is used by biologists in a large number of laboratories for

reconstructing the evolutionary histories of species and inferring the extent and

nature of the selective forces shaping the evolution of genes and species

Creation Of New Alignment

# Select Types of Sequence to be Align

Sequence Retrieving

Sequence Alignment Using ClustalW

ClustalW Parameters Setting

Multiple Sequence Alignment

Export Alignment to MEGA Format

Save MEGA format File

Construction Of Phylogenetic Tree Using

Maximum Likelihood Method

Opening MEGA format File which we Saved

Parameter Setting For Phylogenetic Tree

Progressing Tree

Rectangular Phylogenetic Tree

Showing the Conserved

Residues

Circular Tree Construction

From Rectangular Tree

Final Circular Phylogenetic

Tree After Grouping Genes

Different color represents

different Groups