This is a project report on Magenta Pigment Isolation From Fungus.
1. AN overview project on MAGENTA PIGMENT ISOLATION FROM FUNGUS
FROM SHRM BIOTECH PVT. LIMITED KOLKATA (JULY-AUGUST 2010) submitted by ARKABRATA Bandyapadhyay 3rd YEAR B.Sc (H) IN BIO-TECHNOLOGY Under the supervision of Mr. Ashish Roy (Project Coordinator) REGISTRATION NUMBER: 1586 of 2008-2009 ROLL NUMBER: BUR D BT 2009/O5
Pageno. ABSTRACT INTRODUCTION.. WHY MAGENTA PIGMENT? ................................................................................................... PHISICS OF COLUR.. PIGMENT MAGENTA.. HUE.. CMYK... VARIATION OF MAGENTA.... MAGENTA DYE.. PROCESS MAGENTA. ELECTRIC MAGENTA .. REVIEW OF LITERATURE. HISTORY OF MAGENTA. MAGENTA IN POPULAR CULTURE COMICS BOOK.. BUSINESS... ASTRONOMY. FILM MUSIC.. PARAPSYCHOLOGY. POLITICS. MATERIALS AND METHOD... MATERIALS. INSRUMENT. GLASS WARES. FIBERS CULTURE .. CHEMICALS... METHODS.. PREPARATION (100ml.) OF POTATO DEXTROSE AGAR (PDA)... PREPARATION(100ml.) OF CZAPEKS BROTH MEDIA.. COLLECTING OF FUNGUS.. MAGENTA PIGMENT PRODUCTION PROCESS.. QUANTITATIVE ANALYSIS OFPIGMENT... PURIFICATION OF PIGMENT.. PREPARATION OF SILICA GEL FOR TLC. PREPARATION OF MOBILE PHASE PROCEDURE RETARDATION FACTOR. RESULT AND DISCUSSION OBSERVATION OF MANGO TREES FUNGUS. OBSERVATION OF NEEM TREES FUNGUS. OBSERVATION OF JACK FRUIT TREES FUNGUS. CONCLUTION ADVANTAGES(Health and safety aspects of natural dyes)... LIMITATION... REFERENCES.. EXTARNAL LINKS. ACKNOWLEDGEMENT I owe a great many thanks to a great many people who helped and supported me during my project paper. My deepest thanks to the project coordinator Mr.ASHISH ROY and also my collage H.O.D Dr. Bidyut Bandhopadhaya the Guide of the project for guiding and correcting various documents of mine with attention and care. He has taken pain to go through the project and make necessary correction as and when needed. I express my thanks to the Principal of University ofBurdwan, for extending his support. My deep sense of gratitude to Mr. Ashish Roy my project coordinator and collage H.O.D. Mr. Bidyut Bndhopadhaya,SHRM BIO- TECH pvt. ltd. Kolkata support and guidance. Thanks and appreciation to the helpful people at SHRM BIO- TECH pvt. ltd. Kolkata, for their support. I am greatly obliged to the director of SHRM who accommodated me for the training of the organization. I would also thank my Institution and my faculty members without whom this project would have been a distant reality. I also extend my heartfelt thanks to my family, my parents(Goutam Bandyapadhayay and Anulekha Bandyapadhyay),my sister(Angana Bandyapadhyay) my friends and well wishers. DECLARATION I DO HERE BY DECLARE THAT THIS DISSERTATION REPORT SUBMITTED BY ME ABOUT MAGENTA PIGMENT ISOLATION FROM FUNGUS, IS OF MY OWN AND NOT SUBMITTED TO ANY OTHER INSTITUTION OR PUBLISHED ANYWHERE ELSE. ARKABRATA BANERJEE REG NO. ROLL NO. DATE: ABSTRACT The fungus producing magenta was collected from different tree like mango,neem,jack fruit etc. in nature and culture these fungus from different trees on different PDA(potato dextrose agar) media plate. The fungal strain that produced magenta pigment was closely related to Phoma herbarum. The type of fibers added to Czapeks medium influenced which pigments were produced. The pigment was purified through TLC chromatography. The pigment structure was partially determined. This is the first report of the production of magenta pigment by a microorganism specifically in the presence of nylon-6 fibers, via an unknown mechanism. This phenomenon raises the question of why and how the fungus disperses the pigment inside the fiber and suggests that fabrics can be dyed using microorganisms.
INTRODUCTION WHY MAGENTA PIGMENT?
Demand for natural instead of synthetic pigments for coloring fabrics, foods and cosmetics is increasing. Unlike pigments that are synthetic, those from natural sources allow subtle differences in tone because such pigments generally comprise various color components. Microbes have recently received focus as sources of natural pigments . Many fungal species produce pigments. Monascus fungi produce red or yellow pigments that are used as food colorants. Hahella produces red, prodigiosin, Ashbya produces yellow riboflavin , Phoma produces orange aza-anthraquinone and Chromobacterium produces blue violacein. We discovered a fungus that produces magenta pigment. We found that the produced colors were magenta, pink or green depending on the type of fiber added to the culture medium. These findings indicated that various fibers influence the metabolic pathways involved in the production of secondary pigment metabolites. PHYSICS OF COLOUR : Electromagnetic radiation is characterized by its wavelength and its intensity. When wavelength is with in visible spectrum the range of wavelength human can perceive, approximately from 380 nm to 740 nm. It is known visible light. The science of colour is called as chromatics. PIGMENT : A pigment is the material that changes the colour of light .it reflects as the result of selective colour absorption. This physical process differs from Fluorescence, phosphorescence and other forms of Luminescence in which the material itself emits light. Many materials absorb certain wavelength of light that humans have chosen and develop for use as pigments usually have special properties that make them ideal for coloring other materials. A pigment has a high tinting strength relative to the materials it colors. It must be stable form at ambient temperature. Archaeologists have uncovered evidence that early humans used paint for aesthetic purpose such as body decoration. Pigments and paint grinding equipment believed to be between 350,000 and 400,000 years old have been reported in a cave at twin Rivers, near Lusaka, Zambia. The earliest known pigments were natural minerals. Microbes have recently received focus as sources of natural pigments, many fungal species produce pigments. Monascus fungi produces Red or yellow pigments that are used as food colorants. Hahella produces red, Prodigiosin, Ashbya produces Yellow Riboflavin, and Phoma produces Pink or Orange pigment. MAGENTA : Magenta is a colour made up of equal part of red and blue light. This would be the precise definition of the colour as defind for computer display. It is pure chroma on the colour wheel mid way between violet and rose. In HSV colour space, Magenta has a Hue of 300C. Magenta is not a spectral but an extra spectral colour. It cant be generated by light of single wavelength. Human being can only see as far as 380 nm in to the spectrum i.e. as far as violet. The Hue Magenta is the complement of green. Magenta pigment absorb green light, thus Magenta and green are opposite colour. Magenta actually contains more purple pigment than Fuchsia that is actually used to create Magenta. Magenta was invented in 1980. The colour originally called as Fushin made from coal tar dyes in 1859. Besides being called original magenta, it is also called Rich magenta. It is mixture of red and blue wavelength. HUE : Hue is one of the main properties of a colour defined technically as the degree to which a stimulus can be described as red, green, blue, and yellow. CMYK : The best places to start when trying to understand the colour spectrum is with the CMYK colour model which is composed of cyan that is a bluish green colour, magenta is a purplish red colour, yellow that is medium yellow and key which is black. The CMYK colour model is newer to the industry compared to the more traditional RGB or red, green, blue model. VARIATION OF MAGENTA Magenta is a purplish pink colour evoked by light with less power in yellowish green wavelength then in blue and red wavelength. The hue magenta is the complement of green. Magenta pigment absorb green light, thus magenta and green are opposite colour.
The three variation of magenta pigment are; MAGENTA DYE (ORIGINALVARIATION) PROCESS MAGENTA (PIGMENTMAGENTA) (PRINTER MAGENTA) ELECTRIC MAGENTA (ADDTIVE SECONDARY MAGENTA) 1. MAGENTA DYE : Before printers magenta was invented in the 1890 for CMYK printing and electric magenta was invented in 1980 for computer display. Magenta dye colour is also called Rich Magenta. Magenta was one of the first aniline dyes discovered shortly after the Battle of Magenta (1859). The colour was originally called Fuchine or Roseine, but for marketing purpose in 1860 the colour name was changed to magenta. 2. PROCESS MAGENTA : In colour printing, the colour called Process Magenta or Pigment Magenta. It is one of the primary pigment colours which along with yellow and cyan constitute the 3 subtractive primary colours of pigment. The secondary colours of pigment are blue, green, and red. As such the CMYK printing process was invented in 1890. Process Magenta is not an RGB colour, and there is no fixed conversion from CMYK to RGB. 3. ELECTRIC MAGENTA : For computer colour radiation, that specific Hue of magenta composed of equal part of red and blue light was termed the WEB colour fuchsia and was assigned as an alias for the RGB code of magenta on the list of standardized web colours ELECTRIC Magenta and fuchsia are exactly same colour. Some time Electric Magenta is called ELECTONIC Magenta. Review of literature Dyeing has almost ceased to exist as a traditional art. In this 20th century the importance of colour is our lives seems to be realizing les