LEUKOPOIESIS

I WAYAN SUGIRITAMAhttp://sugiritama.blogspot.com

sugiritama@gmail.com

LEUKOPOIESIS

“Formation and maturation of white blood cells”

• Granulocytopoiesis• Agranulocytopoiesis–Monocytopoiesis– Lymphoctye maturation

NEUTROPHILS

EOSINOPHILS

BASOPHILS

LYMPHOCYTES

MONOCYTES

BONE MARROW : The Site of LeucopoiesisVascular compartment

Extensive network of sinusoids .

Hemopoietic compartmentIslands of hematopoietic cells (Hemopoietic cells in various stage of maturation )

Leukopiesis

• Depends upon the presence of suitable microenvironment

• Regulate by growth factors, CSF• Process :– Mitosis– Differentiation

GRANULOCYTOPOIESIS• Formation of the granulocytes (neuthrophil, eosinophil, and basophil)• All of Granulocyte progenitor cell derive from

CFC-S• Granulocyte Progenitor :– CFU-Eo : eosinophil lineage– CFU-Ba : Basophil lineage– CFU-GM• CFU-G : Neutrophil line• CFU-M : monocyte line

GRANULOCYTOPOIESIS

• Granulocyte maturation divide into six stages• Structural Changes :– A decreased in cell size– Condensation of nuclear chromatin– Changes in nuclear shape– An accumulation of cytoplasmic granule

GRANULOCYTOPOIESIS

MYELOBLAST (1st)

PROMYELOCYTE (2nd)

MYELOCYTE (neutrophil 3rd )

METAMYELOCYTE (neutrophil 4th )

STAB (neutrophil 5th )

SEGMENTED (neutrophil 6th )

Conclusion

• All of the granulocyte derive from myeloblast• Myelocyte is the stage when three types of

granulocyte can be recognized• The maturation process of granulocyte

characterized by :– synthesis of azurophilic and spesific granules– Condensation of nucleus

• Mature granulocyte leave the bone marrow then enter the circulation connective tissue

Agranulopoiesis

• Agranulocytes derive from CFC-S and CFC-Ly• Morphologic changes :– Decrease in overall cell and nuclear diameter– An increase in nuclear chromatin density

MONOBLAST (Monocytopoiesis)

• Large cell (15-25 )• Nucleus oval• Cytoplasm without

granule or few azurophlic granule

• Differentiate to the Promonocyte

PROMONOCYTE(Monocytopoiesis)

• Divide twice in the course of their development into monocytes

MONOCYTE ON BONE MARROW

• Mature monocytes enter the bloodstream, circulate.

• then enter the connective tissues, where they mature into macrophages.

MONOCYTE ON PERIPHER

LYMPHOPOIESIS

• CFU-Ly divides form the CFU-LyB and CFU-LyT• CFU-LyB migrate to “bursa-equivalent

location”, divided B lymphocytes • CFU-LyT undergo mitosis migrate to the

Thymus T lymphocytes

LYMPHOBLAST

LYMPHOCYTE ON BONE MARROW

LYMPHOCYTE

CONCLUSION

• Leucocytosis is formation of WBC, occurs in Bone Marrow

• Granulocyte and monocyte derive from Myeloid stem cells, whereas limfocyte from Lymphoid Stem cell

• Leucocytosis involved the series of mitosis and differentiation

• Leucocytosis are regulated by several growth factor and Interleukin

AGRANULOCYTOSIS

1. Definition ?2. Sign and symptoms ?3. Causes ?4. Patophysiology ?5. Diagnosis ?6. Treatment ?

ANY QUESTION ?