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GATELifeSciences(XL)‐2010 Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/GATE/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 1
GATELifeSciences(XL)2010(GraduateAptitudeTestinEngineering:OriginalQuestionPaper)
Biochemistry(I)OptionalPaper
Questions1–10carries1markeach:
1. Nucleolusisinvolvedinthesynthesisof:a. rRNAb. tRNAc. DNAd. mRNA
2. Intryptophanoperon,tryptophanactsas:a. Repressorb. Activatorc. Co‐repressord. Co‐activator
3. PositiveselectionofTcellsensures:a. MHCrestrictionb. Self‐tolerancec. TCRengagementsd. Activationbyco‐stimulatorysignals
4. ADNAbindingmotifis:a. Helix‐loop‐helixb. Helix‐turn‐helixc. Helicalwheeld. Loop‐helix‐loop
5. AminoacidsresponsibleforN‐linkedandO‐linkedglycosylationofproteinsare:a. AsparagineandAsparticacidb. GlutamineandSerinec. GlutamicacidandSerined. AsparagineandThreonine
6. OneofthefollowingcompoundsisNOTaneurotransmitter:a. Dopamineb. Glutamicacidc. Histidined. Glycine
7. Approximatemolecularweight(kDa)oftheproductaftertranslationofa390basemRNAwillbe:www.ea
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GATELifeSciences(XL)‐2010 Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/GATE/UGC/JRFNETLifeScienceExaminationpleasevisit:
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a. 48b. 26c. 39d. 14
8. Lineweaver‐Burkplotisaplotof:
9. Amixtureofproteins(W,X,Y,Z)eluteformSephadexG‐200columnintheorderW,
X,Y,Z.TheproteinwithmaximumelectrophoreticmobilityonSDS‐PAGEwillbea. Wb. Xc. Yd. Z
10. Specificprecursorofallprostaglandinsis:a. Oleicacidb. Arachidonicacidc. Palmiticacidd. Α‐linolenicacid
Questions11–20carrytwomarkseach
11. Chymotrypsinandlysozymeareinvolvedrespectivelyin:P.RemovalofsuccessivecarboxylterminalresiduesQ.HydrolyticcleavageofpeptidebondR.CleavageofglycosidicC‐ObondS.Oxygentransportinblood
a. P,Qb. Q,Rc. Q,Sd. R,S
12. MatchtheitemsinGroupIwiththoseinGroup2GroupI GroupIIP.Isotypeswitching 1.VHdomainQ.Clonalanergy 2.Non‐responsivetoself‐antigenR.ClassIIMHC 3.Non‐responsiveTHcellsS.Self‐tolerance 4.β2‐microglobulin
a. P–1,Q–4,R–3,S–2www.easy
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GATELifeSciences(XL)‐2010 Savepaper…Savetrees…
FormoreQuestionsandResourcesforyourCSIR/ICMR/DBT/ICAR/GATE/UGC/JRFNETLifeScienceExaminationpleasevisit:
www.easybiologyclass.com 3
b. P–2,Q–4,R–1,S–3c. P–1,Q–3,R–4,S–2d. P–2,Q–1,R–3,S–4
13. MultipleRNApolymerasetranscribesaDNAtemplate,unwindingabout1.5turnsofDNAtemplatepertranscriptionbubble.FromthestructuralinformationofclassicalB‐DNA, how many transcription bubbles are possible for a 180 base pair DNAmolecule?
a. 12b. 27c. 6d. 270
14. MatchtheitemsinGroupIwithmostappropriateseparationtechniqueinGroupIIGroupI GroupIIP.Mixtureofglycineandalbumin 1.GaschromatographyQ.Mixtureof20and60kDaproteins 2.DialysisR.Histonesfromnuclearextract 3.AffinitychromatographyS.Lectins 4.Sizeexclusionchromatography 5.Thinlayerchromatography 6.Cationexchangechromatography
a. P–1,Q–4,R–3,S–5b. P–5,Q–3,R–6,S‐1c. P–2,Q–4,R–6,S‐3d. P–6,Q–5,R–2,S–4
15. Inthetwohalfreactions:Acetaldehyde+2H++2e‐→Ethanol ∆Eo=‐0.16VNAD+H+→NAD++2H++2e‐ ∆Eo=‐0.32V(F=23,063cal/V)The∆Goforthecoupledreactionwillbe:
a. +7400calb. ‐7400calc. ‐22200cald. +22200cal
16. MatchtheparametersinGroupIwiththecorrectoptioninGroup2GroupI Group2P.Km 1.CatalyticefficiencyoftheenzymeQ.Kcat/Km 2.AffinityofenzymetotheinhibitorR.pKa 3.AffinityofenzymetothesubstrateS.K1 4.Maximumbufferingcapacitywww.ea
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GATELifeSciences(XL)‐2010 Savepaper…Savetrees…
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a. P–3,Q–1,R–2,S‐4b. P–3,Q–1,R–4,S‐2c. P–1,Q–2,R–4,S‐3d. P–1,Q–4,R–2,S‐3
17. Theriseperresidueofα‐helixisabout1.5Å.Aproteinspans2nmbilayer,7timesthrough its trans‐membrane α‐helical domain. Approximately, how many aminoacidresiduesconstitutethetrans‐membranedomainoftheprotein.
a. 105b. 450c. 30d. 190
18. MatchtheproteininGroupIwiththeircorrectfunctioninGroup2GroupI GroupIIP.Shakerprotein 1.InnermembranereceptorQ.Bacteriorhodopsin 2.ActivetransportR.Porin 3.VoltagegatedK+channelS.ABCtransporter 4.LightdrivenH+pump 5.Membranefusion 6.β‐barrelsimplediffusionchannel
a. P–4,Q–2,R–3,S–5b. P–5,Q–3,R–4,S–6c. P–6,Q–1,R–5,S–4d. P–3,Q–4,R–6,S–2
19. Themetabolicdisorders,alkaptonuriaandphenylketonuriaarecausedbydefectsintheenzymes:P.Glucose‐6‐phosphateQ.PhenylalaninehydroxylaseHomogentisate1,2‐dioxygenaseS.Tyrosinase
a. Q,Rb. P,Rc. P,Qd. Q,S
20. MatchthemetabolicpathwaysinGroupIwithcorrespondingenzymesinGroupIIGroupI GroupIIP.β‐oxidation 1.RibulosebisphosphatecarboxylaseQ.Glyclysis 2.Phosphofructokinase1www.ea
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GATELifeSciences(XL)‐2010 Savepaper…Savetrees…
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R.Gluconeogenesis 3.PhosphoenolpyruvatecarboxykinaseS.Calvincycle 4.Thiolase 5.Phosphofructokinase2
a. P–4,Q–2,R–3,S–5b. P–3,Q–2,R–4,S–1c. P–3,Q–1,R–5,S–2d. P–4,Q–2,R–3,S–1
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