PowerPoint Presentation
Day 1Introduction 9:00 am to10:30 amFlow Cytometry Training
Robert Salomon (Flow Manager and Senior Flow Cytomerty
Scientist)
Theory Session 0900 till 1300
Introductions to the Lab and Staff Self IntroductionsBasics of
flow cytometryApplications for Flow Cytometry
Morning tea - Provided
Getting StartedPanel Design Controls and compensationData
Analysis and InterpretationData Acquisition
OverviewInstrumentation
Lunch provided
Practical SessionSession 1Outline 5 mins
Day 1
Practical Session 1330 till 1600
Lab orientationKey Cytometry hardware Systems overviewReview of
Acquisition SoftwareMock Experiment
Cytometry Exam to be completed after practical before tomorrows
sessionTraining ScheduleOutline 5 mins
Day 1
Cell Sorting 900 till 1230
Recap, discussion, exam questionsCell SortingAutomacs Pro FACS
(fluorescent Activated Cell Sorting)Troubleshooting
Certificates
Training ScheduleOutline 5 mins
Day 2
Theory Session 0900 till 1300
Introductions to the Lab and Staff Self IntroductionsBasics of
flow cytometryApplications for Flow Cytometry
Morning tea - Provided
Getting StartedPanel Design Controls and compensationData
Analysis and InterpretationData Acquisition
OverviewInstrumentation
Lunch provided
Practical SessionSession 1Outline 5 mins
Day 1
Introduction to Garvan Flow
Robert Salomon
Flow Cytometry Manager Senior Flow Cytometry ScientistISAC
Emerging Leader 2014-2019
[email protected] Lab 92958431Office 92958432Introduction
Flow Staff
Eric LamFlow Cytometry Operator
[email protected] 92958431 Introduction Flow Staff
Vitri DewiFlow Cytometry Technician
[email protected] 92958431
Introduction Flow Staff
Hira Saeed Flow Cytometry Technician
[email protected] 92958431
Introduction Flow Staff
Name and position ?
Group/lab affiliation ?
Flow Experience ?
Brief introduction to your project ?
Introduction Attendees
Basics of Flow Cytometry ?Measurement
METRY
Basics of Flow Cytometry ?Measurement
METRYCells
CYTO
Basics of Flow Cytometry ?Measurement
METRYCells
CYTOFlow Flow
Flow Cytometry Measurement
METRYCells
CYTOFlow Flow
Flow CellHydrodynamic focusing of sample to laser intercept
-(interrogation point)
LegendLaser intercept
Core Stream
Introduction to Flow: animation
Why Use Flow ?Rapid analysis ( 3k- 200k events/second)
Individual event analysis
Quantifiable results
Multiple parameter analysis
Statistical relevance
Why Use Flow ?Rapid analysis ( 3k- 200k events/second)
Individual event analysis
Quantifiable results
Multiple parameter analysis
Statistical relevance
Imaging Flow CytometryCells per field/sec)Approximately
10020,000Number of Parameters
