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Environmental Physiology (FISH441) group presentation of research project
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Abby TillotsonHalley NelsonRizky Darmawan
HYPOXIA IMPACTS ON PACIFIC OYSTER (CRASSOSTREA GIGAS) GENE EXPRESSION
http://osufoundation.org/news/featurednews/current/moore/index.htm
Anthropogenic contribution
CROSS SECTION
Thermo- or Pycnocline
Hypoxia
• Dissolved oxygen concentration <2mg/l•Worldwide• Estuaries• Semi-enclosed seas
• Commonly seasonal• Anthropogenic • Increase in duration, extent and magnitude
Diaz (2001); Diaz and Rosenberg (2008)
LOW DO
BACKGROUND
Dead Zone?
Chronicle/Ken Stevens :Michigan Department of Natural Resources Thousands of gizzard shad were found along the shore (Mona Lake)
AQUATIC
QUESTIONS
Plasticity of Gene Expression?
QUESTIONS
http://en.wikipedia.org/wiki/File:Phenotypic_plasticity_diagram.svg
HYPOXIA
Secondary Ecological Impacts• Heat-shock proteins• Hsp70
QUESTIONS
Secondary Ecological Impacts
• Heat-shock proteins• Hsp70
• Hypoxia-Inducible Factors• HIF-1α
QUESTIONS
Secondary Ecological Impacts
• Heat-shock proteins• Hsp70
• Hypoxia-Inducible Factors• HIF-1α
• Insulin-like Growth Factor• hrIGF-1
http://pubs.niaaa.nih.gov/publications/arh26-4/274-281.htm
QUESTIONS
Hypoxia Exposure
Hypotheses
HIF-1α
Hsp70
High DO Low DO
mR
NA
gen
e e
xpre
ssio
n
Acclimation
hrIGF-1
Pacific Oyster
Methods
Why?• Abundant
• Lives in the intertidal zone
• Commercially important
Experimental Design
Methods
Hsp70
Results
High DO Control Low DO0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
Acclimation
mea
n m
RN
A e
xpre
ssio
n of
Hsp
70
HIF-1α
Results
hrIGF-1
Results
High DO Control Low DO0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
Acclimation
Mean m
RN
A e
xpre
ssio
n o
f hrI
GF-1
Summary
1. Hsp70: General stress response, but lower levels in Low DO acclimated group
2. HIF-1α: Lower levels in Low DO acclimated group
3. hrIGF-1: High levels in Low DO acclimated group
Importance
Adapt
Grow
SurviveCommercial Conservation