EVALUATION OF THE IN VIVO MOISTURIZING EFFICACY OF ALPHA GLUCAN

OLIGOSACCHARIDE USED IN SYNDET BAR

Mohammad Baghaei PharmD1, Mohammad Reza Nateghi MD1, Hamid Reza Zolfaghari MD1, Amir Houshang Ehsani MD2, Behrooz Barikbin MD3

1. Department of R&D, Facedoux Dermocosmetic Co., Tehran, Iran 2. Department of Dermatology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 3. Assistant Professor of Dermatology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Cleansing …

The Greek physician Galen (130-200 AD) and the Iranian eighth century chemist Gabiribne Hayyan were the first to have written about the use of soap as a body-cleansing agent.

Gabiribne Hayyan

The art of cleansing has progressed immensely over several thousand years…

Cleansers in dermatology and cosmetic

Cleanser Composition

The type and amount of surfactant in a cleansing agent has a bearing on its drying and irritancy potential.

Table 1: The constituents of skin cleansers

Evolution of modern day cleansers

Cleansers can be divided into three basic types:

Soaps Syndet bars Combars

Dermatologic Syndet bars(synthetic detergent)

• Dermatologic Syndet (synthetic detergent) bars have a non-soap synthetic surfactant such as fatty acid isothionates, sulfosuccinic acid esters as their principal ingredient.

Syndets have a neutral or slightly acidic pH and are less irritating to skin and do not form soap scum layer.

Cleansing and skin barrier function• Day-to-day challenges, such as changes in

humidity, temperature, and daily cleansing, can affect skin’s barrier properties and its ability to hold water. Hyper-hydration occurring during regular

wash and after extended water exposure is followed by shrinking as water evaporates, leading to drying stresses. Swelling facilitates the penetration of surfactants into deeper layers, possibly leading to a biochemical response such as irritation and itch.

Because surfactant binding reduces the skin’s ability to retain water, skin often returns to a state of lower hydration after washing.

Surfactant binding to SC proteins can cause significant protein denaturation, leading to barrier damage.

The Ecoflora – a microbiological defense barrier

• Mary Marples (The Ecology of the human skin, 1965)

Saprophytic flora and pathogenic flora

• The barrier between saprophytic flora and pathogenic flora is often difficult to define.

Probiotic, Prebiotic

Prebiotic : Non-digestible food ingredient that has a beneficial effect on health by selectively stimulating the growth and/or metabolic activity of one or a limited number of intestinal & Skin bacteria.

Probiotic : Refers to living micro-organisms, generally bacteria, used in foodstuffs in order to enhance the quality of the intestinal flora. In man, bifidus probiotics aid digestion and are said to improve the immune defense system.

BIOECOLIA (Alpha-glucan oligosaccharide)

Its bioselectivity as a substrate will enable it to restrict the growth of pathogenic flora.

It is use in cosmetic product as an emollient and conditioning agent

It is a saccharine (sugar molecule) that has water-binding abilities.

Our study …

• The objective of our study was to evaluate the in vivo moisturizing efficacy of alpha-glucan oligosaccharide components used in syndet bar in healthy female subjects.

Methods & Materials128 healthy female subjects, between 18-65 years of age, having a dry skin, were randomly selected into case and treated groups for this clinical trial study.

Two types of syndet bar composed of Tensianol 815 (as control) and Tensianol plus alpha-glucan oligosaccharide (as experiment) were used.

The syndet bars were applied twice a day as a hand washing by the case and treated groups, over a sixty-day period. The moisturizing effect was evaluated by corneometry at T0, T+2 days, T+5 days and T+60 days.

Data was analyzed by paired and two-tailed t-test.

ResultsMean age of participants was 36.8±10.5

The mean level of skin hydration measured by corneometry was 35.8±5.7 in treated group and 35.9±5.5 in control group at beginning of the study (T0).

The mean level of skin hydration at T+2 and T+5 days after application of syndet bar was 36.1±7.0 and 37.3±10.1 in treated skin and 35.4±6.7 and 35.9±5.1 in control group respectively.

In the treated group, there was no significant difference between baseline measurement (T0) and T+2 and T+5 days measurement (P<0.05). Also there was no significant difference between baseline measurement and T+2 and T+6 days measurement in the control group (P>0.05). Besides, there was no significant difference between treated skin and control skin at T+2 and T+5 days measurement (P<0.05).

Long-term application of syndet bar showed that the mean level of skin hydration in the treated skin at T+60 days was 40.3±8.9 and in the control skin was 35.2±6.4.

Results…

There was significant difference between baseline measurements (T0) and T+60 days in treated groups (P<0.05) but no significant different was seen in control group at T+60 days.

A significant difference was seen between treated group and control group at T+60 days of measurement (P<0.05).

Conclusion

The study showed that application of a syndet bar composed of alpha-glucan oligosaccharide component was not able to improve moisturizing of skin in short-term usage but it is able to improve moisturizing long-term usage (approximately 14.5%). No unwanted or adverse event has been reported by the subjects during the study time course.

It is able to improve moisturizing long-term usage (approximately 14.5%)

It is my pleasure introduce you a new application in dermatological syndet bar field :

It is prebiotic syndet bar

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