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An in depth look at the data life cycle and how BOLD can help people interact globally in relation to barcode projects.
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BOLD and the data life cycle
Julie K. StahlhutBiodiversity Institute of Ontario
With examples from BOLD 3.0 Beta
Study concept
Data collection
Data processing
Data distribution
Data discovery
Data analysis
Data archiving
RepurposingRepurposing
Data life cycle: A common
model
Data Documentation Initiative, 2004(http://www.ddialliance.org/)
What does this mean?
• Every BOLD user is:– A contributor– A consumer– A curator– An improver of existing data.
• Existing BOLD data are:– Records of past research– Resources for future research
Basic life cycle of barcode data
Data generation
Validation
✗
✗
✗
Lab technician
Campaignmanager
You
Upload
Analysis
Hypothesis testing
Context(Ecology, geography, phylogeny)
?
Identification and comparison
= ?
Publication
Project Access:
Make this project publicly visiblex
The truelife
cycle
ValidateValidate
Re-visitRe-visit
GenerateGenerate
PublishPublish
AnalyzeAnalyze
UploadUpload
Re-visited data
Add value
Manageprojects
Plan research
Project manager
Taxonomist
Graduate student
?
?
?
Taxonomic expertise
Taxonomic expertise
Barcode data expertise
Barcode data expertiseFuture expertFuture expert
BOLD tools
1. Adding value to data
Sample code
Country Identification Identifier VoucherLocation
2010-785 Australia Formicidae U. of Adelaide
A2011-Q Australia Formicidae U. of Adelaide
ZYZ-1450 Australia Camponotus sp. N. Novice U. of Adelaide
2002-18xt Australia Formicidae U. of Adelaide
XM20-36 Australia Formicidae U. of Adelaide
ANT-480 Australia Formicidae U. of Adelaide
Good sequences but no IDs.
Good sequences but no IDs.
Sample code
Country Identification Identifier Notes
2010-785 Australia Iridomyrmex purpureus A. Phaenogaster
2011-782 Australia Myrmecia rugosa A. Phaenogaster
ZYZ-1450 Australia Polyrhachis australis A. Phaenogaster Corrected
20018xt2 Australia Linepithema humile A. Phaenogaster Invasive
XM20-36 Australia Strumigenys sp. A. Phaenogaster
ANT-480 Australia Rhytidoponera mayri A. Phaenogaster
Reference quality
barcodes!
Reference quality
barcodes!
2. Research planning
?
?
Molecular
Statistical
Ecological
BARCODING SPECIES
DIVERSITY
BARCODING SPECIES
DIVERSITY
Tropical vs. temperate rainforest
Tropical vs. temperate rainforest
Formicidae vs. ApidaeFormicidae vs. Apidae
Research ProposalLorem Ipsum, M.S.
Collaborators:C.M. Bees, University of GuelphA. Phaenogaster, University of Adelaide
Abstract: Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. BARCODE duis aute irure velit esse dolore eu
Introduction: Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat BOLD. Excepteur sint occaecat
3. Project management Lab
progress
Sequence quality
Collectioncoverage
Dataupdates
Hymenoptera Barcode Group Quarterly Report:
C.M. Bees, Manager
We have met our BARCODE targets for this quarter!
• Australian ant project near completion • New ant and bee diversity survey under way• Collaborators needed in:
• Argentina• Russia• South Africa
Hym
enop
tera
Hym
enop
tera
Bees -> 2100Bees -> 2100
Wasps -> 2800Wasps -> 2800
Ants -> 1950Ants -> 1950
ResearchResearch
Project management
Project managementEducationEducation
BOLD resources
CollaborationCollaboration
At all stages of the data life cycle:
•Data are dynamic, not static.
•Existing BOLD data are resources for future work.
•Collaborations can add value to your BOLD data.
•Your active participation improves data quality and accessibility for everyone!
Generate Validate Upload Analyze Publish Re-visit
Acknowledgements
•BOLD team•Paul Hebert•Suz Bateson•Alex Wild •Clker.com