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Page 1: BT631-1-introduction

BT 631: Protein Structure and Function (3-0-0-6)

Introduction to protein structure and function; amino acids: building blocks of proteins, peptide bonds,

polypeptides and its conformation; structural elements of protein structure: primary, secondary, tertiary

and quaternary structures; bonds stabilizing protein structure; structural motifs; methods of determining

three-dimensional structures of protein: X-ray crystallography, nuclear magnetic resonance (NMR), cryo-

electron microscopy; basic concepts in analyzing protein structures; protein structure database; structure

and function of fibrous proteins: (kerartins and collagens) and membrane proteins (ATPase family);

structures and mechanisms of dehydrogenases and proteases; chemical modification of enzymes using

affinity reagents and side-chain specific reagents.

Text Books/References

• Principles of Protein Structure, G.E. Schulz and R.H. Schirmer, Springer (1979).

• Proteins: Structure and Molecular Properties, Thomas E. Creighton, W.H. Freeman (1992).

• Introduction to Protein Structure, Carl Branden and John Tooze, Garland Science (1999).

• Proteins and Enzymes, JE Bell and ET Bell, Prentice Hall College Div (1988).

• Proteins: Structure and Function, David Whitford, John Wiley (2005).

• Reviews and Articles.

Course Syllabus

Page 2: BT631-1-introduction

S. No. Date/Day Topic to be covered

1. 08.08.2013 Introduction to the course

2. 12.08.2013 Introduction to protein structure and function

3. 13.08.2013 Amino acids

4. 19.08.2013 Peptide bonds

5. 22.08.2013 Polypeptide and its conformations

6. 26.08.2013 Structural elements of protein structure

7. 27.08.2013 ,,

8. 29.08.2013 Bonds stabilizing protein structure

9. 02.09.2013 ,,

10. 03.09.2013 Structural motifs

11. 05.09.2013 ,,

12. 09.09.2013 X-ray crystallography

13. 10.09.2013 ,,

14. 12.09.2013 ,,

15. 16.09.2013 ,,

16. 17.09.2013 NMR

Tentative Class Distribution

Page 3: BT631-1-introduction

S. No. Date/Day Topic to be covered

17. 19.09.2013 ,,

18. 23.09.2013 Mid Sem Exam

19. 24.09.2013 ,,

20. 25.09.2013 ,,

21. 26.09.2013 ,,

22. 27.09.2013 ,,

23. 28.09.2013 ,,

24. 30.09.2013 Cryo EM

25. 01.10.2013 ,,

26. 03.10.2013 Basic concepts in analyzing protein structures

27. 07.10.2013 ,,

28. 08.10.2013 Protein structure database

29. 10.10.2013 Structure and function of fibrous proteins

30. 17.10.2013 Keratins

31. 21.10.2013 ,,

32. 22.10.2013 Collagens

33. 24.10.2013 ,,

Page 4: BT631-1-introduction

S. No. Date/Day Topic to be covered

34. 28.10.2013 Membrane proteins

35. 29.10.2013 ,,

36. 31.10.2013 ATPase family

37. 04.11.2013 ,,

38. 05.11.2013 Structures and mechanisms of dehydrogenases

39. 07.11.2013 ,,

40. 11.11.2013 Structures and mechanisms of proteases

41. 12.11.2013 ,,

42. 18.11.2013 Chemical modifications

43. 19.11.2013 Using affinity reagents

44. 21.11.2013 Side chain specific reagents

45. 25.11.2013 End Sem Exam

46. 26.11.2013 ,,

47. 27.11.2013 ,,

48. 28.11.2013 ,,

49. 29.11.2013 ,,

50. 30.11.2013 ,,

Page 5: BT631-1-introduction

Total No. of Classes available: 38 + 2 (Quizzes)

Quiz No. 1 (10 Marks): on 04.09.2013 (Wednesday Time=4-5PMMid Sem Examination: 40 Marks

Quiz No. 2 (10 Marks): on 26.10.2013 (Saturday) Time=10-11AMEnd Sem Examination: 40 Marks

Total Marks: 100

Marks Distribution


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