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DEPARTMENT OF BICOHEMISTRY

1. Plasma membrane controls a) Absorption of water b) Passage of water and solutes in and out of cell

c) Passage of water and solutes into the cell d) passage of water and solutes out of the cell 2. Within a cell the site of aerobic respiration is

a) Ribosomes b) Mitochondria c) Golgi body d) Endoplasmic reticulum

3. Which of the following cell organelles is considered to be rich in hydrolytic enzymes; a) Endoplasmic reticulum b) Lysosome c) Golgi body d) Mitochondria

4. The largest cell organelle in the cells are a) Plasmids b) Golgie bodies c) Mitochondria d) Chromosomes

5) Chromosomes having equal or almost equal arms are known as a) Metacentric b) Acrocentric c) Concentric d) Telocentric

6) The basic amino acid is

a) Glycine b) Histidine c) Proline d) Serine 7. The carboxyl groups of amino acids exist almost entirely as the conjugate base at pH

a) 6.6 b) 6.8 c) 7.2 d) 7.4

8. The amino acids which synthesize most hormones are a) Histidine b) Phenylalanine c) Valine d) Alanine

9. An amino acid that does not form an α-helix is a) Aspargine b) Proline c) Tyrosine d) Tryptophan.

10. Transamination is a) Reversible process b) Irreversible process c) Both of the above d) None of the above

11. Thiamine disphosphate acts as a coenzyme for

a) Aldolase b) Transaldolase c) Transketolase d) all of the above. 12. NAD is required as a coenzyme for

a) Malate Dehydrogenase b) Succinate Dehydrogenase c) Glucose-6-phosphate Dehydrogenase d)HMG CoA reductase.

13. Pyridoxine deficiency can be diagnosed by measuring urinary excretion of a) Pyruvic acid b) Oxaloacetic acid c) Xanthurenic acid d) None of the above.

14. Transducin is a

a) Signal transducer b) Stimulatory G-protein c) Trimer d) All of the above. 15. Intestinal bacteria can synthesize

a) Vitamin-A b) Vitamin-D c) Vitamin-E d) Vitamin-K.

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16. Calorific value of alcohol is

a) 4 kcal/gm b) 5.4 kcal/gm c) 7 kcal/gm d) 9 kcal/gm

17. Maize is poor in

a) Lysine b) Methionine c) Tryptophan d) Lysine & Tryptophan 18. Milk is poor in

a) Cholesterol b) Retinol c) Calcium d)Iron

19. During starvation, ketone bodies are used as a fuel by a) Erythrocytes b) Brain c) Liver d) All of the above

20. Aminoacids are required to synthesize all of the following except a) Purines b) Pyrimidines c) Pyridoxine d) Heme

21. Muscle phosphorylase is a) A dimer b) Contains vit B6 c) Activated by cAMP d) The serine hydroxyl group of

phosphrylase A

22. HUNTER’s disease is due to deficiency of;

a) Iduronate sulphatase b) Acetyl transferase c) Beta glucoronidase d) Beta

galactosidase

23. Abnormally structured glycogen is seen in

a) Von Gierke’s disease b) Her’s disease c) Pompe’s disease d) Cori’s disease 24. Insulin-stimulated uptake is associated with

a) GLUT-1 b) GLUT-2 c) GLUT-3 d) GLUT-4

25. Ingestion of high fructose syrups lead to all except

a) Increased FA synthesis b) Increased VLDL Secretion

c) Increased esterification of FA d) Decreased LDL

26. Fructosamine is

a) A product formed by interaction of glucose and albumin b) A product formed by interaction of fructose and albumin c) End product of fructose metabolism d) A compound of chondroitin sulphate.

27. Total glycated Haemoglobin level is

a) 4-6 mg% b) 3-8 mg% c) 5-10 mg% d) 2-3 mg% 28. Specific gravity of urine is decreased in

a) Diabetes Mellitus b) Acute glomerulonephritis c) Diarrhoea d) Chronic glomerulonephritis

29. An enzyme which is excreted in urine is a) LDH b) Amylase c) Ornithine transcarbamoylase

d) None of the above

30. At what wavelength DNA absorbs the light at maximum

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a) 260 nm b) 280 nm c) 240 nm d) 300 nm

31. Which type of DNA physiologically exists in Eukaryotes

a) A-DNA b) B-DNA c) Z-DNA d) All the above

32. Which of the following deficiency causes respiratory distress syndrome a) Phosphatidyl Serine b) Phosphatidyl Ethanolamine c) Phosphatidyl Choline d) Phosphatidyl Inositol

33. Which compound is not synthesised from cholesterol

a) Cortisol b) Aldosterone c) Bile acids d) Prostaglandins 34. Which of the following are ω3 fatty acids

a) Linoleic acid b) - Linolenic acid c) Arachidonic acid d) Oleic acid

35. Which one of the following is Sphingophospholipids a) Gangliosides b) Lecithin c) Phosphatidyl Serine d) Phosphatidyl Ethanolamine

36. Which one of the following is an essential fatty acid for mammals

a) Linoleic acid and Arachidonic acid b) Arachidonic acid and -Linolenic acid

c) Linoleic acid and - Linolenic acid d) Only Linolenic acid

37. Eicosanoids are derived from a) 20 carbon Polyenoic fatty acids b) 18carbon Polyenoic fatty acids

c) 22 carbon Polyenoic fatty acids d) 16carbon Polyenoic fatty acids

38. Which groove of DNA Regulates the gene expression, by specific DNA binding proteins? a) Minor groove b) Major groove c) Both minor and major groove d) None of the above

39. Ribozymes are a) RNA b) DNA c) Protein d) Hormones

40. Which one of the following RNA is involved in processing of mRNA and Gene regulation

a) t-RNA b) r- RNA c) Both t-RNA and r-RNA d) Sn RNA

41. Which arm of t-RNA helps it to bind the ribosomes a) Ψ Arm b) D Arm c) Extra Arm d) Anticodons Arm

42. The enzyme Hexokinase is a a) Hydrolase b) Oxidoreductase c) Transferase d) Ligase

43. The following is a proteolytic enzyme: a) Pepsin b) Trypsin c) Chymotrypsin d) All the above

44. When the velocity of an enzymatic reaction is half of Vmax:

a) Substrate concentration is half of Km b) Substrate concentration is equal to Km c) Substrate concentration is twice the Km d) None of the above

45. In competitive inhibition, the inhibitor a) Competes with the enzyme b) Irreversibly binds with the enzyme

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c) Binds with the substrate d) Competes with the substrate 46. Competitive inhibition can be relieved by raising the

a) Enzyme concentration b) Substrate concentration c) Inhibitor concentration d) None of the above

47. When reducing equivalents enter the respiratory chain through NAD, the P: O ratio is a) 1:2 b) 2:1 c) 1:3 d) 3:1

48. Extra mitochondrial NADH can be oxidized in the respiratory chain with the help of

a) Glycerophosphate shuttle b) Malate shuttle c) Either of the above d) None of the above 49. Substrate-linked phophorylation occurs in:

a) Glycolytic pathway b) Citric acid cycle c) Both of the above d) None of the above

50. Reducing equivalents move in the respiratory chain from a) Relatively electronegative to electropositive components b) Lower redox potential to higher redox potential

c) Reduced substrate to oxygen d) All of the above

51. Superoxide radicals can be detoxified by a) Cytochrome c b) Superoxide dismutase c) Both of the above d) None of the above

52. In ß oxidation when acetoacetate is the end product the number of ATP’s produce are a) 33 b) 25 c) 131 d) 129

53. Leuokotrienes are formed only by a) 5-Lipoxygenase b) Phospholipase c) Cyclooxygenase d) Mitochondrial Oxygenases

54. Fatty Liver results due to deficiency of

a) Panthothenic acid b) Lipoprotein Lipase c) Vitamin A d) Deoxycholic acid 55. Chylomicrons are released from the intestinal mucosal cell by

a) Pinocytosis b) Diffusion c) Osmosis d) Endocytosis

56. The rate limiting enzymes of fatty acid synthase is a) Pyruvate Carboxylase b) Melonyl transacylase c) Citrate synthase d) Acetyl CoA Carboxylase

57. Refsum’s disease is due to defect in

a) - Oxidation b) ß- Oxidation c) ω-Oxidation d) None of Above

58. Zellweger’s syndrome is characterized by accumulation of a) Polyenoic acids b) Peroxisomes c) Bile salts d) Cholesterol

59. In a healthy adult, daily formation of bilirubin is a) 50-100 mg b) 100-250 mg c) 9- 12 mg d) 350-500 mg

60. Pre-hepatic jaundice occurs because of a) Increased haemolysis b) Liver damage

c) Biliary obstruction d) None of the above

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