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Antioxidant properties in callus cultures and in intact plant parts of gynura procumbens

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This document is the presentation slide for viva. Its the summary of my research.

Text of Antioxidant properties in callus cultures and in intact plant parts of gynura procumbens

  • 1. ANTIOXIDANT PROPERTIES IN CALLUS CULTURES AND IN INTACT PLANT PARTS OF GYNURA PROCUMBENS VIJENDREN A/L KRISHNAN GS21956 SUPERVISORY COMMITTEE: PROF. DR. MAZIAH MAHMOOD DR. SYAHIDA AHMAD 1

2. Gynura procumbens have been used in folk medicine since ages, however there is no report on quantitative analysis of antioxidant properties in all intact plant parts and also in in-vitro cultures of G. procumbens. Tremendous development in herbal industry, requires more valuable extracts Hence, this research will provide method to produce high amount of cell cultures and lead to improvisation of the antioxidant properties in the cultures. 2 Problem Statement & Significance 3. OBJECTIVE To compare the antioxidant properties in intact plant parts of Gynura procumbens and Gynura bicolor. To study antioxidant properties of Gynura procumbens leaf, stem and root derived callus. To establish callus induction and callus proliferation from leaf, stem and root explants of Gynura procumbens. 3 4. 4 In Thailand, used to treat topical inflammation, rheumatism, and viral ailments on skin. In Indonesia, used to treat fever, rashes, remedy for ringworm infection. (Iskander et al., 2002) Gynura procumbens posses anti-inflammatory, anti- hyperlipidaemic, anti-hypertensive, anti-cancer, and anti- ulcerogenic properties (Zhang and Tan, 2000; Iskander et al., 2002; Kim et al., 2006, Jenie and Meiyanto, 2008) Presence of essential oils, steroids, bitter principles, flavonoids, valepotriates, coumarins, and alkaloid were reported in various organic solvents by TLC. (Iskander et al., 2002) Review literature 5. Kingdom : Plantae Division : Magnoliophyta Class : Magnoliopsida Order : Asterales Family : Asteraceae (alt. compositae) Tribe : Senecioneae Genus : Gynura Species : procumbens 5 (Taxonomy Botanica Sistematica Online, 2009; USDA,ARS, National Genetic Resources Program 2011) 6. 6 Component Content Moisture 7.08% Carbohydrate 0.1968 g glu/ 100g DW Protein 4.51 g/ 100 g DW Lipid 0.023 g/ 100 g DW Abdel-Wahab et al., 2009;Puangpronpitag et al., 2010 Elements Content (%) C 44.36 O 39.92 Mg 0.4 P 0.4 S 0.39 Cl 3.63 K 8.7 Al 0.06 Ca 1.73 7. 7 8. 8 DPPH (BrandWilliamsetal.,1995) Figure 1: Total antioxidant content of aerial parts of G. procumbens (P) and G. bicolor (B) expressed as percent of inhibition using DPPH method. The bar indicates the standard deviation of mean (n = 3)] 9. 9 FRAP (BenzieandStrain, 1999) Figure 2: Total antioxidant content of aerial parts of G. procumbens (P) and G. bicolour (B) expressed as percent of inhibition using FRAP method. The bar indicates the standard deviation of mean (n = 3) 10. 10 Total Phenolic Content (Singleton and Rossi, 1965) Figure 3: Total phenolic content of aerial parts of G. procumbens (G) and G. bicolor (B) expressed as gallic acid equivalent (mg/g FW). The bar indicates the standard deviation of mean (n = 3). 11. 11 Total Flavonoid Content (Zhishenetal.,1999) Figure 4: Total flavonoid content in aerial parts of G. procumbens (G) and G. bicolor (B) expressed as kaempferol equivalent (g/g FW). The bar indicates the standard deviation of mean (n = 3) 12. 12 Figure 5: Total ascorbic acid content in aerial parts of G. procumbens (G) and G. bicolor (B) expressed as ascorbic acid equivalent (g/g FW). The bar indicates the standard deviation of mean (n = 3) Ascorbic acid content (DaviesandMasten,1990) 13. 13 Thin Layer Chromatography Figure 6: Thin layer chromatography performed using ethyl acetate/formic acid/ acetic acid/water, 100:11:11:26 (V/V) as solvent. [(C) Catechin, (H) Hesperitin, (K) Kaempferol, (N) Naringenin, (Q) Quercetin, (R) Rutin, (PR) G. procumbens root, (PS) G. procumbens stem, (PL) G. procumbens leaf, (BR) G. bicolor root, (BS) G. bicolor stem, (BL) G. bicolor leaf] 14. Callus induction of G. procumbens leaf, stem and root Callus proliferation of G. procumbens leaf, stem and root Callus proliferation profile of G. procumbens leaf, stem and root 14 TISSUE CULTURE 15. Figure7: Influenceof auxinson number of daysto callus initiationon G. procumbens leaf, stem and root explants.The results indicate mean standarddeviation(SD). N=10. 15 16. 16 Figure8: Callusinitiationfrom leaf on MS medium supplementedwith 5M of variousauxinsafter 30 daysof culture: (A) 2,4-D, (B) Dic, (C) IAA, (D) IBA, (E) NAA, (F) Pic, (G) MSO. (Bar indicates1 cm) A B C D E F G 17. A B C D E F G 17 Figure9: Callusinitiationfrom stem on MS medium supplementedwith 5M of variousauxinsafter 30 daysof culture: (A) 2,4-D, (B) Dic, (C) IAA, (D) IBA, (E) NAA, (F) Pic, (G) MSO. (Bar indicates1 cm). E 18. 18 Figure10 : Callusinitiationfrom root on MS medium supplementedwith 5M of variousauxinsafter 30 daysof culture: (A) 2,4-D, (B) Dic, (C) IAA, (D) IBA, (E) NAA, (F) Pic, (G) MSO. (Bar indicates1 cm). A B C D E F G 19. 19 Figure11: : Influence of variousauxinson callus initiationof G. procumbens leaf after three sub-culturesfor 30 dayseach passage.Meanswithin columns followedby the same alphabetsare not significantlydifferent at p

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