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Introduction
• Acid –base balance involves the maintenance of internal pH.
• Regulation of acid –base homeostasis is of critical importance to fishes.
• In order to achieve homeostasis, there must be a balance between the intake or production of hydrogen ions and the net removal of hydrogen ions from the body.
CONT….
• A delicate balance of fluids, electrolytes, and acids and bases is required to maintain good health.
• This balance is called Homeostasis
pH Review
• pH = - log [H+]
• H+ is really a proton
• Range is from 0 - 14• If [H+] is high, the solution is acidic; pH < 7
• If [H+] is low, the solution is basic or alkaline ; pH > 7
• Acids are H+ donors.
• Bases are H+ acceptors, or give up OH- in solution.
• Acids and bases can be:
– Strong – dissociate completely in solution
• HCl, NaOH
– Weak – dissociate only partially in solution
• Lactic acid, carbonic acid
Body Fluids
• Intracellular fluid (ICF)– found within the cells of the body– constitutes 2/3 of total body fluid – major cation is potassium
• Extracellular fluid (ECF)– found outside the cells– accounts of 1/3 of total body fluid– major cation is sodium
The Body and pH
• Homeostasis of pH is tightly controlled
• Intracellular fluid = 7.2 – 7.5
• Blood pH = 7.7 – 8.0• < 7.0 or > 8.5 death occurs
• Acidosis (acidemia) below 7.4
• Alkalosis (alkalemia) above 8.3
Small changes in pH can produce major disturbances
• Most enzymes function only with narrow pH ranges
• Acid-base balance can also affect electrolytes (Na+, K+, Cl-)
• Can also affect hormones
The body produces more acids than bases
• Acids take in with foods
• Acids produced by metabolism of lipids and proteins
• Cellular metabolism produces CO2.
• CO2 + H20 ↔ H2CO3 ↔ H+ + HCO3-
How the Body defends against fluctuations in pH
Three Systems in the body:
1.Buffers in the blood
2.Respiration through the gills
3.Excretion by the kidneys
Buffers in the Blood
• Buffers are substances that neutralize acids or bases
• Bicarbonate which is a base and carbonic acid in the body fluids protect the body against changes in acidity
• These buffer systems serve as a first line of defense against changes in the acid-base balance
Respiration through the gills
• Carbon Dioxide which is formed during cellular metabolism forms carbonic acid in the blood decreasing the pH
• When the pH drops respiration rate increases this hyperventilation increases the amount of CO2 exhaled thereby lowering the carbonic acid concentration and restoring homeostasis
Excretion by the Kidneys
• The kidneys play the primary role in maintaining long term control of Acid-Base balance
• The kidney does this by selecting which ions to retain and which to excrete
• The kidneys adjust the body’s Acid-Base balance
Control of Acids
1. Buffer systems:
Take up H+ or release H+ as conditions change
Buffer pairs – weak acid and a base
Results in a much smaller pH change
Bicarbonate buffer
• Sodium Bicarbonate (NaHCO3) and carbonic acid (H2CO3)
• Maintain a 20:1 ratio : HCO3- : H2CO3
HCl + NaHCO3 ↔ H2CO3 + NaCl
NaOH + H2CO3 ↔ NaHCO3 + H2O
Phosphate buffer
• Major intracellular buffer
• H+ + HPO42- ↔ H2PO4-
• OH- + H2PO4- ↔ H2O + H2PO4
2-
Protein Buffers
• Non-bicarbonate buffers (Nbbs) – plasma proteins and hemoglobin.
• Includes hemoglobin, work in blood
• Carboxyl group gives up H+
• Amino Group accepts H+
2. Respiratory mechanisms
• Exhalation of carbon dioxide
• CO2 solubility in water is 25 times higher than that of oxygen ,so CO2 is easily lost to the envt.
• CO2 + H20 ↔ H2CO3 ↔ H+ + HCO3-
• Body pH can be adjusted by changing rate and depth of breathing.
3. Kidney excretion
• Can eliminate large amounts of acid
• Can also excrete base
• Can conserve and produce bicarb ions• Most effective regulator of pH
• If kidneys fail, pH balance fails
Rates of correction
• Buffers function almost instantaneously
• Respiratory mechanisms take several minutes to hours
• Renal mechanisms may take several hours to days
Acid-Base Imbalances
• pH< 7.4acidosis
• pH > 8.3 alkalosis
• The body response to acid-base imbalance is called compensation
• Partial compensation if range is still outside norms.
Compensation
• If underlying problem is metabolic, hyperventilation or hypoventilation can help : respiratory compensation.
• If problem is respiratory, renal mechanisms can bring about metabolic compensation.
There are 4 Types of Acid-base Imbalances
1.Respiratory Alkalosis
2.Respiratory Acidosis
3.Metabolic Alkalosis
4.Metabolic Acidosis
Respiratory Alkalosis
• Is a decrease in CO2, decrease in H+ (Hydrogen ions) and in increase in pH
• This condition can be caused by continuous fish movement or anxiety reaction
• The body compensates by the gills slowing the respiration rate and kidneys excreting more bicarbonate
Respiratory Acidosis
• Is an increase in CO2, an increase in H+ and a decrease in pH
• This condition can be caused by , restrictive or obstructive gill diseases
• The body compensates by the gills increasing respiration rate and the kidneys by conserving bicarbonate ions and increasing renal net acid excretions
Metabolic Alkalosis
• Is a decrease in CO2, a decrease in H+ and an increase in pH
• This condition can be caused by , increased ingestion of alkali
• The body compensates by the gills creating a slow respiration rate and the kidneys excreting more bicarbonate
Metabolic Acidosis
• Is an increase in CO2, and increase in H+ and a decrease in pH
• This condition can be caused by alkaline loss, excess acid production or ingestion
• The body compensates by the gills increasing respiration rate and the kidneys increasing renal net acid excretion
Conclusion
• Fishes are capable of regulating their internal pH using both internal buffering and transepithelial exchanges across the gills.
• Though their Pco2 and HCO3- are well
below those found in mammals, they can tolerate a variety of acid-base challenges.