ACTIVITY REPORT 2010

ACTIVITY REPORT

2010

Content: Annual Activity Report 2010, presented by CIMAR – Associated Laboratory to Fundação para a Ciência e a Tecnologia.

LIST OF CONTENTS

1. GENERAL INFORMATION ............................................................................................................. 1

2. RESEARCH TEAM.......................................................................................................................... 2

3. OBJECTIVES AND ACHIEVEMENTS ............................................................................................. 13

4. ACTIVITIES .................................................................................................................................. 16

5. FUNDING ................................................................................................................................... 18

6. GENERAL INDICATORS ............................................................................................................... 19

7. RESEARCH LINES (THEMATIC AREAS) ........................................................................................ 20

RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems ...................................... 20

RL2 - Environmental Chemistry and Toxicology ........................................................................ 25

RL3 - Biology and Marine Biotechnologies ............................................................................... 29

RL4 - Aquaculture ...................................................................................................................... 34

8. RESEARCH GROUPS ................................................................................................................... 38

Aquaculture Research Group (Aquagroup) ............................................................................... 40

Aquatic Insular Research ........................................................................................................... 48

Biogeographical Ecology and Evolution (BEE) ........................................................................... 54

Biophysics .................................................................................................................................. 62

Cellular and Inorganic Biochemistry (CIB) ................................................................................. 66

Cellular, Molecular and Analytical Studies (LECEMA) ............................................................... 69

Chemical Speciation and Bioavailability (CS&B) ....................................................................... 77

Chemistry and Biological Activity of Marine Natural Products ................................................. 82

Coastal Biodiversity ................................................................................................................... 86

Comparative Molecular Endocrinology (CME) .......................................................................... 96

Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH) .............................. 104

Ecophysiology .......................................................................................................................... 108

Ecotoxicology and Ecology (ECOTOX) ..................................................................................... 114

Ecotoxicology, Genomics and Evolution (LEGE) ...................................................................... 123

Environmental Technologies ................................................................................................... 131

Environmental Toxicology ....................................................................................................... 136

Evolution, Development and Gene Expression (EDGE)........................................................... 143

Fish Nutrition........................................................................................................................... 151

Fisheries Biology and Hydroecology Research Group (FBHRG) .............................................. 156

Fisheries, Biodiversity and Conservation ................................................................................ 160

Functional Biochemistry and Proteomics ............................................................................... 166

Geology and Paleoceanography ............................................................................................. 169

Hydrobiology ........................................................................................................................... 177

Marine Biotechnologies (MarBiotech) .................................................................................... 185

Marine Community Ecology and Evolution (LMCEE) .............................................................. 188

Marine Plant Ecology (ALGAE) ................................................................................................ 196

Microbial Ecology and Evolution ............................................................................................. 201

Modelling and Coastal Management ...................................................................................... 204

Nutrition, Growth and Quality of Fish (LANUCE) .................................................................... 208

Oceanic and Coastal Dynamics ............................................................................................... 216

Pathology ................................................................................................................................ 226

Plant Systematics and Bioinformatics ..................................................................................... 233

Synthesis and Organic Reactivity ............................................................................................ 236

9. OTHER LA ACTIVITIES ...................................................................................................... 241

9a. Services and resources ...................................................................................................... 241

9b. Networking actions ........................................................................................................... 243

9c. Training .............................................................................................................................. 245

9d. Outreach/science and society ........................................................................................... 247

9e. International events .......................................................................................................... 249

10. INTERNAL EVALUATIONS .............................................................................................. 251

SCIENTIFIC OUTPUT 2009 (Publications, Advanced Training, Communications, Patents)

Evaluation Report 2010

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1. GENERAL INFORMATION

1.1 Name of Research Unit

CIMAR - Centro de Investigação Marinha e Ambiental

1.2 Coordinator

João José Oliveira Dias Coimbra

1.3 Main Scientific Domain

Marine Sciences

1.4 Leading Hosting Institution

Centro Interdisciplinar de Investigação Marinha e Ambiental – CIIMAR

1.5 Other Insitutions involved

Centro de Ciências do Mar – CCMAR


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2. RESEARCH TEAM

2.1 Member researchers with PhD

- 1. Adelino Vicente Mendonça Canário (Agregação / Professor Catedrático / 50%) - 2. Aires Manuel Pereira Oliva Teles (Agregação / Professor Catedrático / 50%) - 3. Anake Kijjoa (Agregação / Professor Catedrático / 60%) - 4. Deborah Mary Power (Agregação / Professor Catedrático / 50%) - 5. Eduardo Jorge Sousa Rocha (Agregação / Professor Catedrático / 40%) - 6. Fernando Francisco Machado Veloso Gomes (Agregação / Professor Catedrático / 60%) - 7. Francisco de Almeida Taveira Pinto (Agregação / Professor Catedrático / 50%) - 8. Helena Maria Leite Pato Granja (Agregação / Professor Catedrático / 50%) - 9. João José Oliveira Dias Coimbra (Agregação / Professor Catedrático / 70%) - 10. José Pedro de Andrade e Silva Andrade (Agregação / Professor Catedrático / 50%) - 11. Lúcia Maria das Candeias Guilhermino (Agregação / Professor Catedrático / 60%) - 12. Maria Leonor Quintais Cancela Fonseca (Agregação / Professor Catedrático / 50%) - 13. Rogério Alves Ferreira Monteiro (Agregação / Professor Catedrático / 40%) - 14. Vitor Manuel Oliveira Vasconcelos (Agregação / Professor Catedrático / 70%) - 15. Adriano Agostinho Donas Bôto Bordalo e Sá (Agregação / Professor Associado / 40%) - 16. Alexandre Manuel Silva Lobo Cunha (Agregação / Professor Associado / 40%) - 17. António José Rogeiro Gouveia (Agregação / Professor Associado / 50%) - 18. Karim Erzini (Agregação / Professor Associado / 33%) - 19. Luísa Maria Pinheiro Valente (Agregação / Professor Associado / 75%) - 20. Maria Leonor Nunes Ribeiro Cruzeiro (Agregação / Professor Associado / 50%) - 21. Maria Lurdes Santos Cristiano (Agregação / Professor Associado / 50%) - 22. Maria Margarida M. Castro (Agregação / Professor Associado / 50%) - 23. Maria Natividade Ribeiro Vieira (Agregação / Professor Associado / 50%) - 24. Rui Orlando Pimenta Santos (Agregação / Professor Associado / 50%) - 25. Ana Isabel de Melo Azevedo Neto (Agregação / Professor Auxiliar / 50%) - 26. Aurélia Maria de Pinho Marques Saraiva (Agregação / Professor Auxiliar / 50%) - 27. Paulo José de Azevedo Pinto Rema (Agregação / Professor Auxiliar / 50%) - 28. Carlos José Correia de Azevedo (Agregação / Aposentado/Jubilado / 80%) - 29. Jorge Guimarães da Costa Eiras (Agregação / Aposentado/Jubilado / 50%) - 30. Maria Armanda Reis Henriques (Agregação / Aposentado/Jubilado / 50%) - 31. Maria Teresa Dinis (Agregação / Aposentado/Jubilado / 75%) - 32. Maria Teresa Sá Dias de Vasconcelos (Agregação / Aposentado/Jubilado / 40%) - 33. António Manuel Santos Afonso (Doutoramento / Professor Associado / 50%) - 34. Isabel Maria Trigueiros Sousa Pinto Machado (Doutoramento / Professor Associado / 50%) - 35. João Paulo de Sousa Cabral (Doutoramento / Professor Associado / 35%) - 36. Jorge Pereira Machado (Doutoramento / Professor Associado / 60%) - 37. José Carlos Pinto Bastos Teixeira da Silva (Doutoramento / Professor Associado / 50%) - 38. Manuel Aureliano Pereira Martins Alves (Doutoramento / Professor Associado / 50%) - 39. Maria Alexandra Anica Teodósio Chicharo (Doutoramento / Professor Associado / 50%) - 40. Maria João Tome Costa Sousa Rocha (Doutoramento / Professor Associado / 40%) - 41. Paulo Manuel Rodrigues Vaz-Pires (Doutoramento / Professor Associado / 50%) - 42. Pedro Manuel Silva Duarte (Doutoramento / Professor Associado / 30%) - 43. Rodrigo Jorge Fonseca de Oliveira Maia (Doutoramento / Professor Associado / 60%) - 44. Alberto Teodorico Rodrigues Moura Correia (Doutoramento / Professor Auxiliar / 30%) - 45. Ana Carolina Tavares Estima Santos da Cunha Feming (Doutoramento / Professor Auxiliar /

40%) - 46. António Paulo Alves Ferreira de Carvalho (Doutoramento / Professor Auxiliar / 50%) - 47. Carla Batista Carvalho Batista Pinto (Doutoramento / Professor Auxiliar / 30%)


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- 48. Cristina Maria Bravo de Faria Cruz (Doutoramento / Professor Auxiliar / 50%) - 49. Dina Cristina Fernandes Rodrigues da Costa Simes (Doutoramento / Professor Auxiliar / 50%) - 50. Eduardo Nuno Picoto Lopes Barata (Doutoramento / Professor Auxiliar / 50%) - 51. Gerhard Michael Weber (Doutoramento / Professor Auxiliar / 30%) - 52. Graça Maria Figueiredo Casal (Doutoramento / Professor Auxiliar / 50%) - 53. João Carlos Serafim Varela (Doutoramento / Professor Auxiliar / 50%) - 54. João Pedro Gomes Moreira Pêgo (Doutoramento / Professor Auxiliar / 60%) - 55. José Alberto Álvares Pereira Gonçalves (Doutoramento / Professor Auxiliar / 30%) - 56. José Américo Pereira de Sousa (Doutoramento / Professor Auxiliar / 50%) - 57. José Carlos Fernandes Antunes (Doutoramento / Professor Auxiliar / 50%) - 58. José Fernando Magalhães Gonçalves (Doutoramento / Professor Auxiliar / 30%) - 59. Luisa Paula Viola Afonso Barreira (Doutoramento / Professor Auxiliar / 50%) - 60. Manfred Josef Kaufmann (Doutoramento / Professor Auxiliar / 40%) - 61. Margarida de Lurdes de Jesus Bastos Cristo (Doutoramento / Professor Auxiliar / 50%) - 62. Maria Antónia Santos Mendes Salgado (Doutoramento / Professor Auxiliar / 35%) - 63. Maria Clara Gomes Quadros Lázaro da Silva (Doutoramento / Professor Auxiliar / 50%) - 64. Maria Clara Ramalho Monteiro Pires Basto (Doutoramento / Professor Auxiliar / 50%) - 65. Maria Clara Semedo da Silva Costa (Doutoramento / Professor Auxiliar / 40%) - 66. Maria Ester Tavares Alvares Serrao (Doutoramento / Professor Auxiliar / 50%) - 67. Maria Joana Afonso Pereira Fernandes (Doutoramento / Professor Auxiliar / 50%) - 68. Maria João Faria Leite Dias dos Santos (Doutoramento / Professor Auxiliar / 50%) - 69. Maria Manuela Fraga Juliano (Doutoramento / Professor Auxiliar / 50%) - 70. Maria Margarida da Fonseca e Castro Cardoso (Doutoramento / Professor Auxiliar / 30%) - 71. Maria Teresa Calvinho Cerveira Borges (Doutoramento / Professor Auxiliar / 50%) - 72. Paula Cristina Paulo Videira da Silva (Doutoramento / Professor Auxiliar / 40%) - 73. Paulo Alexandre de Avilez Rodrigues de Almeida Valente (Doutoramento / Professor Auxiliar /

60%) - 74. Paulo José Relvas Almeida (Doutoramento / Professor Auxiliar / 50%) - 75. Paulo Manuel Rodrigues Martins da Costa (Doutoramento / Professor Auxiliar / 50%) - 76. Pedro Miguel Leal Rodrigues (Doutoramento / Professor Auxiliar / 50%) - 77. Rita Castilho (Doutoramento / Professor Auxiliar / 50%) - 78. Ronaldo Gomes de Sousa (Doutoramento / Professor Auxiliar / 50%) - 79. Sérgio dos Reis Marques Madeira (Doutoramento / Professor Auxiliar / 30%) - 80. Teresa Isabel Mendonça Modesto (Doutoramento / Professor Auxiliar / 50%) - 81. Paulo Jorge Rosa Santos (Doutoramento / Professor Auxiliar Convidado / 50%) - 82. Albina Dolores Cardoso da Silva Castro Resende (Doutoramento / Professor Coordenador /

30%) - 83. Eduardo Bruno Oliveira Esteves (Doutoramento / Professor-Adjunto Equiparado / 50%) - 84. Maria Alexandra de Sousa Lourenço Nunes (Doutoramento / Professor-Adjunto / 50%) - 85. Maria do Rosário Fidalgo Martins (Doutoramento / Professor-Adjunto / 50%) - 86. Maria Dulce da Mota Antunes de Oliveira Estêvão (Doutoramento / Professor-Adjunto / 40%) - 87. Maria Luisa Machado Cerqueira Bastos (Doutoramento / Investigador Principal / 50%) - 88. Agostinho Antunes Pereira (Doutoramento / Investigador Auxiliar / 100%) - 89. Alexandre Marmoto de Oliveira Campos (Doutoramento / Investigador Auxiliar / 100%) - 90. Ana Maria Ferreira Bio (Doutoramento / Investigador Auxiliar / 100%) - 91. Ana Paula de Campos Mucha (Doutoramento / Investigador Auxiliar / 100%) - 92. Carlos Alexandre Sarabando Gravato (Doutoramento / Investigador Auxiliar / 100%) - 93. Cristina Marisa Ribeiro de Almeida (Doutoramento / Investigador Auxiliar / 100%) - 94. Cymon John Cox (Doutoramento / Investigador Auxiliar / 100%) - 95. Elsa Maria Branco Froufe Andrade (Doutoramento / Investigador Auxiliar / 100%) - 96. Filipe Alexandre Oliveira dos Santos Alberto (Doutoramento / Investigador Auxiliar / 100%) - 97. Francisco Arenas Parra (Doutoramento / Investigador Auxiliar / 100%) - 98. Gareth Anthony Pearson (Doutoramento / Investigador Auxiliar / 100%)


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- 99. Iacopo Bertocci (Doutoramento / Investigador Auxiliar / 100%) - 100. Isabel Gonçalves de Barbosa Araújo (Doutoramento / Investigador Auxiliar / 100%) - 101. João Carlos dos Reis Cardoso (Doutoramento / Investigador Auxiliar / 100%) - 102. João Miguel Sousa da Silva (Doutoramento / Investigador Auxiliar / 100%) - 103. Jonathan Mark Wilson (Doutoramento / Investigador Auxiliar / 100%) - 104. Jorge Manuel dos Santos Gonçalves (Doutoramento / Investigador Auxiliar / 100%) - 105. Jorge Proença Dias (Doutoramento / Investigador Auxiliar / 100%) - 106. Juan Fuentes Diaz (Doutoramento / Investigador Auxiliar / 100%) - 107. Kim Richard Larsen (Doutoramento / Investigador Auxiliar / 100%) - 108. Laura Maria Simões Coutinho Guimarães (Doutoramento / Investigador Auxiliar / 100%) - 109. Luís Eugénio Castanheira da Conceição (Doutoramento / Investigador Auxiliar / 100%) - 110. Luís Filipe Costa Castro (Doutoramento / Investigador Auxiliar / 100%) - 111. Machiel Simon Bos (Doutoramento / Não aplicável (bolseiro) / 100%) - 112. Maria Helena Tabuaço Rêgo Martins Peres (Doutoramento / Investigador Auxiliar / 100%) - 113. Marta Mega Rufino (Doutoramento / Investigador Auxiliar / 100%) - 114. Marta Sofia Sá Ferreira (Doutoramento / Investigador Auxiliar / 100%) - 115. Miguel Alberto Fernandes Machado e Santos (Doutoramento / Investigador Auxiliar / 100%) - 116. Paulo Jorge Travessa Gavaia (Doutoramento / Investigador Auxiliar / 100%) - 117. Pedro Miguel Guerreiro Costa Guerreiro (Doutoramento / Investigador Auxiliar / 100%) - 118. Peter Colin Hubbard (Doutoramento / Investigador Auxiliar / 100%) - 119. Radhouane Ben Hamadou (Doutoramento / Investigador Auxiliar / 100%) - 120. Ralph Urbatzka (Doutoramento / Investigador Auxiliar / 100%) - 121. Rodrigo da Silva Costa (Doutoramento / Investigador Auxiliar / 100%) - 122. Rodrigo Ozório (Doutoramento / Investigador Auxiliar / 100%) - 123. Rui Miguel Andrade Caldeira (Doutoramento / Investigador Auxiliar / 100%) - 124. Susana Maria Pereira da Costa Moreira (Doutoramento / Investigador Auxiliar / 100%) - 125. Vicent Laizé (Doutoramento / Investigador Auxiliar / 100%) - 126. Rosa Carmina Cervantes Cianca (Doutoramento / Investigador / 100%) - 127. Ana Margarida Araújo Amaral (Doutoramento /Técnico Superior e Técnico / 100%) - 128. Paula Raquel da Silva Jorge Coutinho Ferreira (Doutoramento / Técnico Superior e Técnico /

100%) - 129. Fernanda Russell Pinto (Doutoramento / Aposentado/Jubilado / 60%) - 130. Alexandra Paula Mimoso Henriques Cunha (Doutoramento / Não aplicável (bolseiro) /

100%) - 131. Alfredo Nuno Damasceno Pinto de Oliveira (Doutoramento / Não aplicável (bolseiro) /

100%) - 132. Amalia Pérez Jiménez (Doutoramento / Não aplicável (bolseiro) / 100%) - 133. Ana Alexandra Pedrosa Ramos (Doutoramento / Não aplicável (bolseiro) / 100%) - 134. Ana Filipa Naughton Henriques Andrez (Doutoramento / Não aplicável (bolseiro) / 100%) - 135. Ana Luisa Frazão Pereira (Doutoramento / Não aplicável (bolseiro) / 100%) - 136. Ana Mafalda Saraiva Baptista (Doutoramento / Não aplicável (bolseiro) / 100%) - 137. António de Vilhena Andrade Ferreira Sykes (Doutoramento / Não aplicável (bolseiro) /

100%) - 138. Aschwin Hillebrand Engelen (Doutoramento / Não aplicável (bolseiro) / 100%) - 139. Carla Alexandra São Bento Viegas (Doutoramento / Não aplicável (bolseiro) / 100%) - 140. Carla Marília Abreu Freitas (Doutoramento / Não aplicável (bolseiro) / 100%) - 141. Catarina Cortes Valente de Oliveira (Doutoramento / Não aplicável (bolseiro) / 100%) - 142. Catarina Isabel de Matos Martins (Doutoramento / Não aplicável (bolseiro) / 100%) - 143. Catarina Maria Pinto Mora Pinto de Magalhães (Doutoramento / Não aplicável (bolseiro) /

100%) - 144. Cláudia Raquel Cêa de Aragão Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%) - 145. Cristina Isabel Coelho Dias Lopes (Doutoramento / Não aplicável (bolseiro) / 100%) - 146. Daniel António Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%)


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- 147. Dulce Isabel Assis Alves Martins (Doutoramento / Não aplicável (bolseiro) / 100%) - 148. Eva Cacabelos Reyes (Doutoramento / Não aplicável (bolseiro) / 100%) - 149. Fernando Cánivas García (Doutoramento / Não aplicável (bolseiro) / 100%) - 150. Francisco Miguel de Sousa Leitão (Doutoramento / Não aplicável (bolseiro) / 100%) - 151. Gustavo Oliveira de Meneses Martins (Doutoramento / Não aplicável (bolseiro) / 100%) - 152. Holly Freedman (Doutoramento / Não aplicável (bolseiro) / 100%) - 153. Isabel Cristina Oliveira Gonzalez Cunha (Doutoramento / Não aplicável (bolseiro) / 100%) - 154. Isabelle Martins Gil (Doutoramento / Não aplicável (bolseiro) / 100%) - 155. Joana Costa Vilhena de Bessa Campos (Doutoramento / Não aplicável (bolseiro) / 100%) - 156. Joana Fernandes da Fonseca da Costa Martins Osswald (Doutoramento / Não aplicável

(bolseiro) / 100%) - 157. Joana Ferreira Marques Ferreira Cardoso (Doutoramento / Não aplicável (bolseiro) / 100%) - 158. João Lemos Gomes Canning Clode (Doutoramento / Não aplicável (bolseiro) / 100%) - 159. João Luis Vargas de Almeida Saraiva (Doutoramento / Não aplicável (bolseiro) / 100%) - 160. João Sollari Allegro Machado Lopes (Doutoramento / Não aplicável (bolseiro) / 100%) - 161. Jorge Afonso Martins da Palma (Doutoramento / Não aplicável (bolseiro) / 100%) - 162. Justyna Kopecka-Pilarczyk (Doutoramento / Não aplicável (bolseiro) / 100%) - 163. Katy Nicastro (Doutoramento / Não aplicável (bolseiro) / 100%) - 164. Larraitz Garmendia (Doutoramento / Não aplicável (bolseiro) / 100%) - 165. Laurence Alexandrine Mathilde Deloffre (Doutoramento / Não aplicável (bolseiro) / 100%) - 166. Ledicia Rey Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%) - 167. Leonardo Filipe Rodrigues da Mata (Doutoramento / Não aplicável (bolseiro) / 100%) - 168. Luís Miguel dos Santos Russo Vieira (Doutoramento / Não aplicável (bolseiro) / 100%) - 169. Luisa Margarida Batista Custodio (Doutoramento / Não aplicável (bolseiro) / 100%) - 170. Manuel Gesto Rodríguez (Doutoramento / Não aplicável (bolseiro) / 100%) - 171. Marcelino Miguel Guedes de Jesus Oliveira (Doutoramento / Não aplicável (bolseiro) /

100%) - 172. Marco António do Nascimento Sequeira de Jesus Campinho (Doutoramento / Não aplicável

(bolseiro) / 100%) - 173. Marcos Rubal Garcia (Doutoramento / Não aplicável (bolseiro) / 100%) - 174. María Asunción Lago Lestón (Doutoramento / Não aplicável (bolseiro) / 100%) - 175. Maria de Fátima Azevedo Alexandrino Fernandes (Doutoramento / Não aplicável (bolseiro) /

100%) - 176. Maria del Mar Huertas Pau (Doutoramento / Não aplicável (bolseiro) / 100%) - 177. Maria Elena Varela Alvarez (Doutoramento / Não aplicável (bolseiro) / 100%) - 178. Maria Emília Carvalho Salgueiro (Doutoramento / Não aplicável (bolseiro) / 100%) - 179. Maria João Fernandes Martins (Doutoramento / Não aplicável (bolseiro) / 100%) - 180. Maria Purificación Veiga Sanchez (Doutoramento / Não aplicável (bolseiro) / 100%) - 181. Maria Regina Oliveira Lopes da Cunha (Doutoramento / Não aplicável (bolseiro) / 100%) - 182. Maria Teresa Garrett Silveirinha Sottomayor Neuparth (Doutoramento / Não aplicável

(bolseiro) / 100%) - 183. Mercedes Gonzalez Wanguemert (Doutoramento / Não aplicável (bolseiro) / 100%) - 184. Nadège Richard (Doutoramento / Não aplicável (bolseiro) / 100%) - 185. Natércia Maria da Silva Conceição (Doutoramento / Não aplicável (bolseiro) / 100%) - 186. Nikolay Kolmakov (Doutoramento / Não aplicável (bolseiro) / 100%) - 187. Nuna Cláudia Peixoto de Araújo (Doutoramento / Não aplicável (bolseiro) / 100%) - 188. Onno Everhardus Diekmann (Doutoramento / Não aplicável (bolseiro) / 100%) - 189. Patrícia Isabel Silvestre Pinto (Doutoramento / Não aplicável (bolseiro) / 100%) - 190. Paula Cristina Enes Oliveira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%) - 191. Pedro Miguel Coutinho Victorino Borges Morais (Doutoramento / Não aplicável (bolseiro) /

100%) - 192. Pedro Nuno da Costa Leão (Doutoramento / Não aplicável (bolseiro) / 100%) - 193. Raquel Castro Alves Ferreira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%)


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- 194. Rita Alexandra Duarte Borges (Doutoramento / Não aplicável (bolseiro) / 100%) - 195. Rui Manuel do Amaral Branco de Oliveira Quartau (Doutoramento / Não aplicável (bolseiro)

/ 100%) - 196. Rui Pedro Andrade Coelho (Doutoramento / Não aplicável (bolseiro) / 100%) - 197. Rui Pedro Gonçalves Pereira (Doutoramento / Não aplicável (bolseiro) / 100%) - 198. Rute Andreia Rodrigues da Fonseca (Doutoramento / Não aplicável (bolseiro) / 100%) - 199. Rute Sofia Tavares Martins Brazona (Doutoramento / Não aplicável (bolseiro) / 100%) - 200. Sandra Cristina da Costa e Silva Ramos (Doutoramento / Não aplicável (bolseiro) / 100%) - 201. Sara Judite Lopes Teixeira (Doutoramento / Não aplicável (bolseiro) / 100%) - 202. Sara Maria Mira da Silva (Doutoramento / Não aplicável (bolseiro) / 100%) - 203. Simone Cavenati (Doutoramento / Não aplicável (bolseiro) / 100%) - 204. Sofia Alexandra Dias Engrola (Doutoramento / Não aplicável (bolseiro) / 100%) - 205. Sonia Martinez Paramo (Doutoramento / Não aplicável (bolseiro) / 100%) - 206. Susana Isabel dos Santos Cabaço (Doutoramento / Não aplicável (bolseiro) / 100%) - 207. Susana Rodrigues Pereira (Doutoramento / Não aplicável (bolseiro) / 100%) - 208. Teodor Ludmilov Stoichev (Doutoramento / Não aplicável (bolseiro) / 100%) - 209. Teresa Alexandra Ribeiro Rodrigues (Doutoramento / Não aplicável (bolseiro) / 100%) - 210. Teresa Paula Martins Tiago (Doutoramento / Não aplicável (bolseiro) / 100%) - 211. Tida Dethoup (Doutoramento / Não aplicável (bolseiro) / 100%) - 212. Vijayakumar Parameswaran (Doutoramento / Não aplicável (bolseiro) / 100%) - 213. Zardi Gerardo (Doutoramento / Não aplicável (bolseiro) / 100%) - 214. Sérgia Catarina de Amorim Costa Dias (Doutoramento / Não aplicável (estudante) / 100%) - 215. Maria da Nazaré Parada Figueiredo de Sousa Couto (Doutoramento / Não aplicável

(investigador) / 95%)

2.2 Member researchers without PhD

- 1. Cristina Maria Viegas Inácio (Mestrado / Técnico Superior e Técnico / 100%) - 2. Filipe Marco Andrade Alves (Mestrado / Técnico Superior e Técnico / 75%) - 3. Hugo Gabriel Maia da Silva Santos (Mestrado / Técnico Superior e Técnico / 100%) - 4. João Carlos Pereira Morais (Mestrado / Técnico Superior e Técnico / 100%) - 5. Marta Sofia Mendes Valente Bernardo (Mestrado / Técnico Superior e Técnico / 100%) - 6. Miguel Ângelo Salgado dos Santos (Mestrado / Técnico Superior e Técnico / 30%) - 7. Patrícia Reis da Silva (Mestrado / Técnico Superior e Técnico / 100%) - 8. Pedro Alexandre Pereira dos Reis (Mestrado / Técnico Superior e Técnico / 100%) - 9. Sofia Ferreira da Rosa Gonçalves (Mestrado / Técnico Superior e Técnico / 100%) - 10. Susana Micaela Machado Ferreira do Vale (Mestrado / Técnico Superior e Técnico / 100%) - 11. Allan Tainá de Souza (Mestrado / Não aplicável (bolseiro)/ 100%) - 12. Ana Isabel Delfin dos Santos Alexandre (Mestrado / Não aplicável (bolseiro) / 100%) - 13. Ana Isabel Duarte André (Mestrado / Não aplicável (bolseiro) / 100%) - 14. Ana Sofia Cardoso Mesquita (Mestrado / Não aplicável (bolseiro)/ 100%) - 15. Ana Vanessa Basto Regueiras (Mestrado / Não aplicável (bolseiro) / 100%) - 16. André Sucena Afonso (Mestrado / Não aplicável (bolseiro)/ 100%) - 17. Andreia Lúcia Gonçalves Adrião (Mestrado / Não aplicável (bolseiro) / 100%) - 18. Andreia Seia Rebotim (Mestrado / Não aplicável (bolseiro) / 100%) - 19. Aurélie Pinto Rodrigues (Mestrado / Não aplicável (bolseiro)/ 100%) - 20. Bárbara Bilreiro de Mendoça Frazão (Mestrado / Não aplicável (bolseiro)/ 100%) - 21. Benjamín Costas Refojos (Mestrado / Não aplicável (bolseiro) / 100%) - 22. Bruno Pessoa Lopes Claro (Mestrado / Não aplicável (bolseiro) / 100%) - 23. Buga Berkovic (Mestrado / Não aplicável (bolseiro) / 100%) - 24. Catarina da Rocha Cruzeiro (Mestrado / Não aplicável (bolseiro)/ 100%) - 25. Catarina Fernanda de Carvalho Pinheiro Teixeira (Mestrado / Não aplicável (bolseiro) / 100%)


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- 26. Catarina Figueiredo da Mota (Mestrado / Não aplicável (bolseiro) / 100%) - 27. Catarina Vizetto Guerreiro Duarte (Mestrado / Não aplicável (bolseiro) / 100%) - 28. Cátia Isabel Moura da Silva (Mestrado / Não aplicável (bolseiro) / 100%) - 29. Chiara Maria Angela Coniglione (Mestrado / Não aplicável (bolseiro) / 100%) - 30. Chommanard Sumngern (Mestrado / Não aplicável (bolseiro) / 100%) - 31. Cristiana Ivone Tavares Moreira (Mestrado / Não aplicável (bolseiro)/ 100%) - 32. Cristiane Cassiolato Pires Hardoim (Mestrado / Não aplicável (bolseiro)/ 100%) - 33. Cristovão José Martins Nunes (Mestrado / Não aplicável (bolseiro) / 100%) - 34. David Maria Aguiar Abecasis (Mestrado / Não aplicável (bolseiro) / 100%) - 35. Diogo Filipe da Silva Gonçalves Soares Paulo (Mestrado / Não aplicável (bolseiro) / 100%) - 36. Edison Samir Mascarenhas Carvalho (Mestrado / Não aplicável (bolseiro) / 100%) - 37. Emanuel Filipe Escaleira Maldonado (Mestrado / Não aplicável (bolseiro) / 100%) - 38. Eva Catarina Costa Amorim (Mestrado / Não aplicável (bolseiro) / 100%) - 39. Fátima de Carvalho Vaz Pinto (Mestrado / Não aplicável (bolseiro) / 100%) - 40. Francisca Isabel Merino Nunes Cabral Cavaleiro (Mestrado / Não aplicável (bolseiro) / 100%) - 41. Franscisco Cid Gonçalves Mont’ Alverne Rocha Pires (Mestrado / Não aplicável (bolseiro) /

100%) - 42. Gisela João Ribeiro Lemos Dionisio (Mestrado / Não aplicável (bolseiro) / 100%) - 43. Guilherme Moura Paredes (Mestrado / Não aplicável (bolseiro)/ 100%) - 44. Guillermin Aguero Chapin (Mestrado / Não aplicável (bolseiro) / 100%) - 45. Helena Isabel Costa de Oliveira (Mestrado / Não aplicável (bolseiro)/ 100%) - 46.Hugo Galvão Caiano Pereira (Mestrado / Não aplicável (bolseiro) / 100%) - 47. Hugo Manuel da Silva Ribeiro (Mestrado / Não aplicável (bolseiro) / 100%) - 48. Imran Khan (Mestrado / Não aplicável (bolseiro) / 100%) - 49. Inês Maria dos Santos Guerreiro (Mestrado / Não aplicável (bolseiro) / 100%) - 50. Joana do Passo Carneiro Azevedo (Mestrado / Não aplicável (bolseiro)/ 100%) - 51. Joana Fernandez de Carvalho (Mestrado / Não aplicável (bolseiro)/ 100%) - 52. Joana Ferreira Costa (Mestrado / Não aplicável (bolseiro) / 100%) - 53. Joana Isabel Correia Bondoso (Mestrado / Não aplicável (bolseiro) / 100%) - 54. Joana Maria Reis Franco Cruz (Mestrado / Não aplicável (bolseiro) / 100%) - 55. Joana Ruela Boavida (Mestrado / Não aplicável (bolseiro) / 100%) - 56. João Miguel Martins Novaes Pinto da Costa (Mestrado / Não aplicável (bolseiro) / 100%) - 57. João Pedro Gonçalves Cardeira da Silva (Mestrado / Não aplicável (bolseiro) / 100%) - 58. José Carlos de Morais Martins (Mestrado / Não aplicável (bolseiro) / 100%) - 59. Juliana Marina Moreira Mendes (Mestrado / Não aplicável (bolseiro)/ 100%) - 60. Kartik Sunagar (Mestrado / Não aplicável (bolseiro) / 100%) - 61. Laura Diniz Leite (Mestrado / Não aplicável (bolseiro)/ 100%) - 62. Luis Gabriel da Silva Luis Laura Diniz Leite (Mestrado / Não aplicável (bolseiro)/ 100%) - 63. Mahaut Diane Marie Stephanie de Labroue de Vareilles Sommieres (Mestrado / Não aplicável

(bolseiro) / 100%) - 64. Márcio André Guerreiro Coelho (Mestrado / Não aplicável (bolseiro)/ 100%) - 65. Margarida Dulce da Conceição Aragão Hermida (Mestrado / Não aplicável (bolseiro)/ 100%) - 66. Maria Alexandra Santos Martins (Mestrado / Não aplicável (bolseiro)/ 100%) - 67. Maria Catarina Murteira Rico dos Santos Campos (Mestrado / Não aplicável (bolseiro) /

100%) - 68. Maria Filipa Bento de Oliveira Falcão Castanheira (Mestrado / Não aplicável (bolseiro)/ 100%) - 69. Mariana Fonseca Hinzmann (Mestrado / Não aplicável (bolseiro) / 100%) - 70. Marina N’Deye Pereira Silva (Mestrado / Não aplicável (bolseiro) / 100%) - 71. Marina Tamagnini Mendes (Mestrado / Não aplicável (bolseiro) / 100%) - 72. Martina Di Iulio Ilarri (Mestrado / Não aplicável (bolseiro)/ 100%) - 73. Micaela de Fátima da Rocha Mota (Mestrado / Não aplicável (bolseiro)/ 100%) - 74. Miguel Albergaria Furtado Semedo (Mestrado / Não aplicável (bolsa)/ 100%) - 75. Miguel Filipe Ferreira da Costa Ramos (Mestrado / Não aplicável (bolseiro) / 100%)


8

- 76. Miguel José Teodoro Correia (Mestrado / Não aplicável (bolseiro) / 100%) - 77. Monya Mendes Costa (Mestrado / Não aplicável (bolseiro) / 100%) - 78. Nelson Alexandre Castilho Coelho (Mestrado / Não aplicável (bolseiro)/ 100%) - 78. Nelson Gonçalo Mortágua Gomes (Mestrado / Não aplicável (bolseiro)/ 100%) - 80. Odete Marinho Gonçalves (Mestrado / Não aplicável (bolseiro) / 100%) - 81. Parthibaraj Anoop Alex (Mestrado / Não aplicável (bolseiro)/ 100%) - 82. Patrícia Alexandra Correia Oliveira (Mestrado / Não aplicável (bolseiro)/ 100%) - 83. Patrícia Isabel Laginha Pereira da Silva (Mestrado / Não aplicável (bolseiro)/ 100%) - 84. Paulo Alexandre Frias Fernandes (Mestrado / Não aplicável (bolseiro)/ 100%) - 85. Paulo Jorge Machado Teixeira de Morais (Mestrado / Não aplicável (bolseiro)/ 100%) - 86. Pedro Alexandre Coelho Borges (Mestrado / Não aplicável (bolseiro) / 100%) - 87. Pedro Miguel Meireles Pinto (Mestrado / Não aplicável (bolseiro) / 100%) - 88. Pedro Neves de Carvalho (Mestrado / Não aplicável (bolseiro) / 100%) - 89. Raquel Andreia Pinheiro Vieira (Mestrado / Não aplicável (bolseiro) / 100%) - 90. Renata Isabel de Sousa Gonçalves (Mestrado / Não aplicável (bolseiro)/ 100%) - 91. Ricardo Mário Bastos Leite (Mestrado / Não aplicável (bolseiro) / 100%) - 92. Richard Deurloo (Mestrado / Não aplicável (bolseiro) / 100%) - 93. Rita Alves Costa (Mestrado / Não aplicável (bolseiro) / 100%) - 94. Rui Alexandre Mendes Gonçalves Mendes (Mestrado / Não aplicável (bolseiro) / 100%) - 95. Rute Sofia Borlido Fiúza Fernandes Pinto (Mestrado / Não aplicável (bolseiro) / 100%) - 96. Siby Philip (Mestrado / Não aplicável (bolseiro)/ 100%) - 97. Silvia Filipa Vicente Gregório (Mestrado / Não aplicável (bolseiro)/ 100%) - 98. Sofia Ester e Sousa de Aguilar Dias (Mestrado / Não aplicável (bolseiro)/ 100%) - 99. Sofia Gabriel Garcia Santos (Mestrado / Não aplicável (bolseiro) / 100%) - 100. Sofia Isabel Franco Cavaco (Mestrado / Não aplicável (bolseiro) / 100%) - 101. Sónia Maria de Sousa Olim (Mestrado / Não aplicável (bolseiro) / 100%) - 102. Sonia Rodriguez Benitez (Mestrado / Não aplicável (bolseiro) / 100%) - 103. Susana Maria Rocha Pina (Mestrado / Não aplicável (bolseiro) / 100%) - 104. Tânia Ribeiro Pinheiro (Mestrado / Não aplicável (bolseiro) / 100%) - 105. Vânia Cristina Palma Roberto (Mestrado / Não aplicável (bolseiro) / 100%) - 106. Vânia de Jesus Paiva Freitas (Mestrado / Não aplicável (bolseiro) / 100%) - 107. Vera Alexandra Garcia da Fonseca Batista (Mestrado / Não aplicável (bolseiro) / 100%) - 108. Vera Lúcia Fernandes Rodrigues (Mestrado / Não aplicável (bolseiro) / 100%) - 109. Virgínia Filipa Pereira Monteiro da Cunha (Mestrado / Não aplicável (bolseiro) / 100%) - 110. Vitor Oliveira Lopes (Mestrado / Não aplicável (bolseiro)/ 100%) - 111. Américo da Silva Magalhães (Mestrado / Não aplicável (estudante)/ 30%) - 112. Ana Rita Ferreira Patarra (Mestrado / Não aplicável (estudante)/ 100%) - 113. Dalila do Rosário Encarnação Serpa (Mestrado / Não aplicável (estudante)/ 50%) - 114. Luciana Paiva das Neves (Mestrado / Não aplicável (estudante)/ 100%) - 115. Marisa Alexandra Marques de Freitas (Mestrado / Não aplicável (estudante)/ 100%) - 116. Nelson Ribeiro Pires (Mestrado / Não aplicável (estudante)/ 50%) - 117. Afonso Costa Lucas Prestes (Licenciatura / Investigador / 100%) - 118. Eunice Rute Magalhães Nogueira Xavier (Licenciatura / Investigador / 100%) - 119. Maria Margarida Oliveira Maló Machado (Licenciatura / Investigador / 60%) - 120. Nuno Miguel da Silva Ascensão Vaz Álvaro (Licenciatura / Investigador / 100%) - 121. Ana Isabel Carvalho Machado (Licenciatura / Técnico Superior e Técnico / 100%) - 122. Ana Margarida Brites Caetano Dinis (Licenciatura / Técnico Superior e Técnico / 75%) - 123. Andreia Sofia Pires Pinto (Licenciatura / Técnico Superior e Técnico / 100%) - 124. Isabel Regal Teixeira (Licenciatura / Técnico Superior e Técnico / 100%) - 125. Jimmy Melro James (Licenciatura / Técnico Superior e Técnico / 100%) - 126. João Eugénio Bernardino Pena dos Reis (Licenciatura / Técnico Superior e Técnico / 100%) - 127. Liliane Leman (Licenciatura / Técnico Superior e Técnico / 80%)


9

- 128. Pedro António Nobre Soares Pinto das Neves (Licenciatura / Técnico Superior e Técnico / 30%)

- 129. Pedro Miguel Gama Cláudio (Licenciatura / Técnico Superior e Técnico / 100%) - 130. Tânia Sofia da Silva Guerreiro (Licenciatura / Técnico Superior e Técnico / 100%) - 131. Francisco Ricardo Lacerda Branco (Licenciatura / Técnico Superior e Técnico / 100%) - 132. Alexandra Isabel Rodrigues Filipe (Licenciatura / Não aplicável (bolseiro) / 100%) - 133. Lopes Ismael (Licenciatura / Não aplicável (bolseiro) / 100%) - 134. Amnat Eamvijarn (Licenciatura / Não aplicável (bolseiro) / 100%) - 135. Ana Catarina da Cruz Gonçalves Café (Licenciatura / Não aplicável (bolseiro) / 100%) - 136. Ana Cristina Pereira da Silva Gonçalves Balau (Licenciatura / Não aplicável (bolseiro) / 100%) - 137. Ana Cristina Silva Rocha (Licenciatura / Não aplicável (bolseiro) / 100%) - 138. Ana Filipa dos Santos Gonçalves (Licenciatura / Não aplicável (bolseiro) / 100%) - 139. Ana Isabel dos Santos Esteves (Licenciatura / Não aplicável (bolseiro) / 100%) - 140. Ana Isabel dos Santos Couto (Licenciatura / Não aplicável (bolseiro) / 100%) - 141. Ana Mafalda Rocha Tavares (Licenciatura / Não aplicável (bolseiro) / 100%) - 142. Ana Mafalda Tomás Correia (Licenciatura / Não aplicável (bolseiro) / 100%) - 143. Ana Margarida Pinto Henrique Machado (Licenciatura / Não aplicável (bolseiro) / 100%) - 144. Ana Rita Andrade Coelho (Licenciatura / Não aplicável (bolseiro) / 100%) - 145. Ana Rita Guillot Caldas (Licenciatura / Não aplicável (bolseiro) / 100%) - 146. Anabela Ben-Simon Brito (Licenciatura / Não aplicável (bolseiro) / 100%) - 147. Anabela Rodrigues Lopes (Licenciatura / Não aplicável (bolseiro) / 100%) - 148. André Gonçalo Antunes dos Santos (Licenciatura / Não aplicável (bolseiro) 100%) - 149. Astrid Yazmine Mejia Martinez (Licenciatura / Não aplicável (bolseiro) 100%) - 150. Brigite Sandra Nunes Simões (Licenciatura / Não aplicável (bolseiro) / 100%) - 151. Bruno Emanuel Pereira Louro (Licenciatura / Não aplicável (bolseiro) / 100%) - 152. Catarina Dias Cavaleiro (Licenciatura / Não aplicável (bolseiro) / 100%) - 153. Célia Teresa Neto dos Santos (Licenciatura / Não aplicável (bolseiro) / 100%) - 154. Cindy Vitória Fazenda (Licenciatura / Não aplicável (bolseiro) / 100%) - 155. Cláudia Filipa Devesa André Moreira (Licenciatura / Não aplicável (bolseiro) / 100%) - 156. Cláudia Sofia Patrão Beliz Rosa e Silva (Licenciatura / Não aplicável (bolseiro) / 100%) - 157. Conceição Alexandra Costa Caetano (Licenciatura / Não aplicável (bolseiro) / 100%) - 158. Cristina Prates Laranjinha Ventura (Licenciatura / Não aplicável (bolseiro) / 100%) - 159. Daniela da Silva Lima (Licenciatura / Não aplicável (bolseiro) / 100%) - 160. Eduardo Bruno de Freitas Vivas (Licenciatura / Não aplicável (bolseiro) / 100%) - 161. Elisabete Alexandra Dias de Matos (Licenciatura / Não aplicável (bolseiro) / 100%) - 162. Fabiana Encarnação Pinto Freitas (Licenciatura / Não aplicável (bolseiro) / 100%) - 163. Filipe André Moreira de Figueiredo (Licenciatura / Não aplicável (bolseiro) / 100%) - 164. Francisco Luís Wallenstein Faria e Maia de Macedo (Licenciatura / Não aplicável (bolseiro) /

100%) - 165. Gonçalo Jorge Franco Silva (Licenciatura / Não aplicável (bolseiro) / 100%) - 166. Izabela Luiza Reis (Licenciatura / Não aplicável (bolseiro) / 100%) - 167. Joana Alexandra Teixeira Rosa (Licenciatura / Não aplicável (bolseiro) / 100%) - 168. Joana Faria da Costa (Licenciatura / Não aplicável (bolseiro) / 100%) - 169. Joana Reis de Almeida (Licenciatura / Não aplicável (bolseiro) / 100%) - 170. Joana Silveira Soares (Licenciatura / Não aplicável (bolseiro) / 100%) - 171. João Miranda Neiva (Licenciatura / Não aplicável (bolseiro) / 100%) - 172. João Paulo Rodrigues Machado (Licenciatura / Não aplicável (bolseiro) / 100%) - 173. Jorge Manuel Ferreira de Assis (Licenciatura / Não aplicável (bolseiro) / 100%) - 174. José Maria Peiro Crespo (Licenciatura / Não aplicável (bolseiro) / 100%) - 175. Lília Isabel Lameirinhas Cabral (Licenciatura / Não aplicável (bolseiro) / 100%) - 176. Luci Catarina Agostinho Jesus (Licenciatura / Não aplicável (bolseiro) / 100%) - 177. Manuel Peixoto de Magalhães Lopes Lima (Licenciatura / Não aplicável (bolseiro) / 100%) - 178. Manuela Maria Neiva Maranhão (Licenciatura / Não aplicável (bolseiro) / 100%)


10

- 179. Marco Alexandre Cavaco Cerqueira (Licenciatura / Não aplicável (bolseiro) / 100%) - 180. Maria Inês de Almeida Páscoa (Licenciatura / Não aplicável (bolseiro) / 100%) - 181. Maria Joao Rodrigues Pereira (Licenciatura / Não aplicável (bolseiro) / 50%) - 182. Maria Patrícia Frojan Fuentes (Licenciatura / Não aplicável (bolseiro) / 100%) - 183. Marisa Sárria Pereira de Passos (Licenciatura / Não aplicável (bolseiro) / 100%) - 184. Marta Isabel da Silva Rafael (Licenciatura / Não aplicável (bolseiro) / 100%) - 185. Michael Nogueira Viegas (Licenciatura / Não aplicável (bolseiro) / 100%) - 186. Monica Sofia Furtado Martins (Licenciatura / Não aplicável (bolseiro) / 100%) - 187.Olga María Hernández Martínez (Licenciatura / Não aplicável (bolseiro) / 100%) - 188. Patricia Alexandra Cavaleiro Diogo (Licenciatura / Não aplicável (bolseiro) / 100%) - 189. Patrícia Isabel da Mota e Silva (Licenciatura / Não aplicável (bolseiro) / 100%) - 190. Paula Liliana Vila Nova Salgado (Licenciatura / Não aplicável (bolseiro) / 100%) - 191. Pedro Filipe dos Santos Palma (Licenciatura / Não aplicável (bolseiro) / 100%) - 192. Pedro Filipe Duarte Alves da Veiga (Licenciatura / Não aplicável (bolseiro) / 100%) - 193. Rita Isabel Pontes Barbosa Colen (Licenciatura / Não aplicável (bolseiro) / 100%) - 194. Rui Carlos Pinto Borges (Licenciatura / Não aplicável (bolseiro) / 100%) - 195. Rula Dominguez Fernandez (Licenciatura / Não aplicável (bolseiro) / 100%) - 196. Sheila Natalí Estrada Allis (Licenciatura / Não aplicável (bolseiro) / 100%) - 197. Silvia Maria dos Santos Albano (Licenciatura / Não aplicável (bolseiro) / 100%) - 198. Sofia Raquel Soares Mesquita (Licenciatura / Não aplicável (bolseiro) / 100%) - 199. Sónia Margarida Fernandes Manso (Licenciatura / Não aplicável (bolseiro) / 100%) - 200. Suradet Buttachon (Licenciatura / Não aplicável (bolseiro) / 100%) - 201. Tânia Raquel Santos Aires (Licenciatura / Não aplicável (bolseiro) / 100%) - 202. Tomé Pereira de Azevedo Santos Silva (Licenciatura / Não aplicável (bolseiro) / 100%) - 203. Vitor Manuel Capela Ramos (Licenciatura / Não aplicável (bolseiro) / 100%) - 204. Viviana Raquel Vieira da Silva Lopes (Licenciatura / Não aplicável (bolseiro) / 100%) - 205. Wanida Auamcharoen (Licenciatura / Não aplicável (bolseiro) / 100%) - 206. Wilson Gabriel Poseiro Coutinho Pinto (Licenciatura / Não aplicável (bolseiro) / 100%) - 207. Adriano Miguel Ricardo Andrade (Licenciatura / Não aplicável (estudante) / 40%) - 208. Ana Campos da Costa e Silva (Licenciatura / Não aplicável (estudante) / 50%) - 209. Ana Sofia da Silva Azevedo (Licenciatura / Não aplicável (estudante) / 40%) - 210. André Pinhal Gonçalves (Licenciatura / Não aplicável (estudante) / 100%) - 211. Andreia Mota Rego (Licenciatura / Não aplicável (estudante) / 40%) - 212. Bernardo Bordalo Domingues dos Santos (Licenciatura / Não aplicável (estudante) / 100%) - 213. Carla Patrícia Rodrigues Olim (Licenciatura / Não aplicável (estudante) / 50%) - 214. Carlos José Magalhães e Silva (Licenciatura / Não aplicável (estudante) / 100%) - 215. Carlos Miguel Henriques Ferreira (Licenciatura / Não aplicável (estudante) / 50%) - 216. Carolina Machado Malheiro Rodrigues (Licenciatura / Não aplicável (estudante) / 100%) - 217. Carolina Oliveira Castro (Licenciatura / Não aplicável (estudante) / 50%) - 218. Cláudia Santos Ferreira (Licenciatura / Não aplicável (estudante) / 50%) - 219. Cristiana Andreia Valente Oliveira (Licenciatura / Não aplicável (estudante) / 100%) - 220. Cristiano Joaquim Gonçalves Freitas (Licenciatura / Não aplicável (estudante) / 30%) - 221. David Lopes Calvão (Licenciatura / Não aplicável (estudante) / 40%) - 222. Diogo Manuel Andrade da Silva (Licenciatura / Não aplicável (estudante) / 75%) - 223. Elsa Maria da Silva Carvalho (Licenciatura / Não aplicável (estudante) / 60%) - 224. Filipa Isabel da Rocha Cardoso (Licenciatura / Não aplicável (estudante) / 50%) - 225. Florbela Alexandra Silva Vieira Martins (Licenciatura / Não aplicável (estudante) / 100%) - 226. Francisco de Assis Medeiros dos Santos (Licenciatura / Não aplicável (estudante) / 100%) - 227. Francisco Rosário de Figueiredo (Licenciatura / Não aplicável (estudante) / 50%) - 228. Helena Rita de Carvalho Ferraz Pedrosa Teodósio (Licenciatura / Não aplicável (estudante) /

100%) - 229. Ildefonso José Vital Simões (Licenciatura / Não aplicável (estudante) / 100%) - 230. Ivone da Silva Pinheiro (Licenciatura / Não aplicável (estudante) / 30%)


11

- 231. João Faria de Oliveira Santos (Licenciatura / Não aplicável (estudante) / 100%) - 232. João Manuel Almeida Antunes Coelho (Licenciatura / Não aplicável (estudante) / 20%) - 233. João Rodrigo Gonçalves Goiana Mesquita (Licenciatura / Não aplicável (Estudante) / 10%) - 234. Jorge Tiago Vieira Ferreirinha Antunes (Licenciatura / Não aplicável (estudante) / 50%) - 235. José Luís das Dores Sousa (Licenciatura / Não aplicável (estudante) / 100%) - 236. Juliana Andreia Silva da Cunha (Licenciatura / Não aplicável (estudante) / 50%) - 237. Liliana Isabel Tomé dos Anjos Guerreiro (Licenciatura / Não aplicável (estudante) / 100%) - 238. Marcelo José Seabra Azevedo (Licenciatura / Não aplicável (estudante) / 100%) - 239. Marcos Lobo de Sousa (Licenciatura / Não aplicável (estudante) / 50%) - 240. Maria João Santos de Almeida (Licenciatura / Não aplicável (estudante) / 40%) - 241. Maria Lígia da Silva Sousa (Licenciatura / Não aplicável (estudante) / 50%) - 242. Maria Sofia Ramos Costa (Licenciatura / Não aplicável (estudante) / 50%) - 243. Marisa Pinto da Silva (Licenciatura / Não aplicável (estudante) / 100%) - 244. Paula Alexandra Rodrigues e Araújo Guedes (Licenciatura / Não aplicável (estudante) /

100%) - 245. Pedro Miguel Gouveia Santos (Licenciatura / Não aplicável (estudante) / 50%) - 246. Pedro Miguel Moreira Araújo (Licenciatura / Não aplicável (estudante) / 50%) - 247. Pedro Silva Vilares (Licenciatura / Não aplicável (estudante) / 100%) - 248. Ricardo Manuel Campinho Capela (Licenciatura / Não aplicável (Estudante) / 75%) - 249. Ricardo Reis Albes Soares Cardoso (Licenciatura / Não aplicável (estudante) / 40%) - 250. Rute Sofia Barros Pinto (Licenciatura / Não aplicável (estudante) / 100%) - 251. Sílvia Araújo Santos (Licenciatura / Não aplicável (estudante) / 50%) - 252. Susana Luísa Amaral da Cruz (Licenciatura / Não aplicável (estudante) / 40%) - 253. António Marco Oliveira Cunha (Ensino secundário / Técnico Superior e Técnico / 100%) - 254. Helena Sofia Fernandes Teixeira (Ensino secundário / Técnico Superior e Técnico / 100%) - 255. Marcos António Viegas Granja (Ensino secundário / Técnico Superior e Técnico / 100%) - 256. Ana Moreira Silva (Ensino secundário / Não aplicável (bolseiro) / 25%) - 257. Hugo Emanuel Santos Vidal (Ensino secundário / Não aplicável (bolseiro) / 20%) - 258. Marisa Sofia Sousa Oliveira (Ensino secundário / Não aplicável (bolseiro) / 20%) - 259. Ricardo José da Silva Castro (Ensino secundário / Não aplicável (bolseiro) / 25%) - 260. Tânia Isabel Boto Sérgio (Ensino secundário / Não aplicável (bolseiro) / 100%) - 261. Andreia Raquel da Costa e Silva (Ensino secundário / Não aplicável (estudante) / 50%) - 262. Filipa João de Sousa (Ensino secundário / Não aplicável (estudante) / 50%) - 263. Francisco António Baptista Fernandes (Ensino secundário / Não aplicável (estudante) / 25%) - 264. Francisco Miguel de Sousa Ferreira (Ensino secundário / Não aplicável (estudante) / 50%) - 265. Joana Catarina Pereira Jorge Fonseca da Rocha (Ensino secundário / Não aplicável

(estudante) / 50%) - 266. Joana Maria Soares Pereira (Ensino secundário / Não aplicável (estudante) / 100%) - 267. Patricio José Bastos Gonçalves (Ensino secundário / Não aplicável (estudante) / 50%) - 268. Tiago André Machado Ribeiro (Ensino secundário / Não aplicável (estudante) / 50%)


12

2.3 Summary

215; 45%

116; 24%

135; 28%

16; 3%

Academic degree

PhD

Master

Graduate

Undergraduate

Figure 1. Distribution of Member researchers according to academic degree.

Figure 2. Distribution of Member researchers - PhD holders according to professional category.


13

3. OBJECTIVES AND ACHIEVEMENTS

3.1 Unit description

CIMAR Associated Laboratory (CIMAR LA) was initiated in 2002 as a partnership between CIIMAR (University of Porto) and CCMAR (University of Algarve). CIMAR LA is directed by a managing board composed by six members, three from each of the two partner institutions. The Director of CIIMAR is the head of the managing board and President of CIMAR LA and the Vice-Vice-President is the Director of CCMAR. Board members meet regularly personally or via videoconference to analyze progress and strategic planning. CIIMAR and CCMAR are two separate legal entities and management and implementation of administrative tasks must be carried out separately and in coordination. The leaders at the two centers are in permanent contact ensuring effective management of CIMAR LA.

CIMAR LA integrates 33 research groups, scientifically organized in 5 research lines each with two coordinators: 1) Ecology, Biodiversity and Management of Aquatic Ecosystems; 2) Environmental Chemistry and Toxicology; 3) Biology and Marine Biotechnologies; 4) Aquaculture; 5) Physical Oceanography, Coastal Dynamics and Engineering. Line 5) has been recently added. CIMAR, LA offers internal and external services for analytical chemistry and molecular biology and genetics.

CIMAR LA has five horizontal programmes: 1) Public awareness of science; 2) Post-graduate studies; 3) Thematic networks; 4) Technology transfer; 5) Support to public policies.

CIMAR LA established formal links with the Research Unit on Eco-Ethology (UIEE) at the Higher Institute of Applied Ethology (ISPA) focusing on behavioral endocrinology, evolutionary biology and conservation and an application to FCT has been made for UIEE to become the third partner institution of CIMAR LA. From very early stage CIMAR LA also established formal institutional links with the Marine Geology department of LNEG (former IGM), covering aspects related to geochemistry and climate change. This cooperation translated in a significant level of collaborative research projects and post graduate student supervision. .

CIMAR LA has a cooperation agreement with CESAM-LA (University of Aveiro) to develop and implement a large collaborative program that includes a Joint Doctoral Program in Marine and Environmental Sciences (launched in 2007/2008), a coastal monitoring program and a capacity building program aimed at Portuguese speaking countries.

The CIMAR LA network was also enlarged by regional agreements with several research institutions, such as ISR – Institute of Systems and Robotics and INEGI – Institute for institute for mechanical engineering and industrial management, in Porto. Two small CIMAR research groups were setup in the Madeira and Azores Archipelagos.

3.2 General objectives

The main objectives of CIMAR LA are “to understand natural processes in the ocean and the coastal zone, to study and implement sustainable exploitation of aquatic resources and to


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characterize and monitor the impact of human activities in the environment”. With these objectives from the initial 10 lines of research, 5 lines as indicated above have consolidated.

CIMAR LA is distributed in different regions covering distinct biogeographical areas - north and south of Portugal, Madeira and the Azores. This allows for effective monitoring of the coastal environments in these regions (North of Portugal and Algarve), in the context of nature conservation, the impact of human activities, and the sustainability of aquaculture and fisheries. International networking activities are an important component of CIMAR LA activity as can be witnessed by the presence in several international research and infrastructure projects and networking, concerted and support actions.

One of the strategic objectives of CIMAR LA is to extend scientific cooperation and training to tropical and subtropical regions, especially Portuguese speaking countries. This objective has had limited success largely by the lack of availability of sponsoring programmes supporting this kind of action.

The permeation of scientific results to society is an important objective which is achieved through: a) intensification of links with companies and the promotion of innovation, technology transfer and entrepreneurship; b) Outreach activities through interaction with schools, “Ciência Viva” centres, and the wider public; c) promoting networking activities among the scientific communities and various stakeholders; d) training of Masters and Doctoral students , post-graduate courses and summers schools; e) the support public policies.

3.3 Main achievements during the year of 2010

NUMBERS

Steady increase in scientific output, despite 2010 lagged 10% below the exceptional 2009. Even so, the number ISI indexed papers in 2010 was 5% greater than in 2008 and 26% higher than in 2007. Intergroup publications stabilized at levels of the most recent years (aprox. 10% of sci. output). Networking actions paralleled 2009, which represents a rise of 36% vs 2008. As to funding, the LA FCT baseline support for 2010 was at the level of previous years, but it was followed by a significantly higher input via competitive money, particularly via FCT projects, which generated 6% more in budget in 2010 vs 2009. Overall, funding was maintained.

RL1

Knowledge on marine evolutionary ecology and biogeography, and population dynamics of a range of species.

Assessment of the ecological status of transitional waters and development of tools towards the implementation of the WFD.

Improved knowledge of effects of climate events, ocean acidification, temperature, precipitation and introduced/invasive species.


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Enlargement of a computation cluster, now providing a platform for genome assembly phylogenetic analyses.

Discovery and description of new species, mapping of biodiversity and identification of essential habitats in coastal waters, contributions and creation of data bases.

RL2

Developed analytical techniques to measure environmental contaminants and cyanobacteria toxins, and their environmental degradation.

Demonstration that the retinoic acid receptor RXR is a key player in imposex induction by the androgenic chemical tributyltin.

Validation of methods and integrated strategies to assess the effects and risks of global climate changes, pollutants, biotoxins, and other stressors on marine ecosystems biodiversity, functioning and evolution were validated, using a molecular to ecosystem level approach, also clarifying mechanisms of toxicity.

RL3

Application of high throughput methods of transcriptome analyses (microarray, SAGE, 454 sequencing), tackling physiological challenges and novel genes in teleosts and one Antarctic bivalve. Studies proofing the concept of allying “new generation sequencing” to PCR and bioinformatics.

A draft sequence of the European sea bass genome was advanced, along with a draft regulatory network of genes involved in ion homeostasis in enterobacteria. Perkinsus genome was completed more fish pathogenic agents of economic interest were identified.

RL4

A main achievement was a steady contribution to the establishment of intensive and semi-intensive farming systems for new species in the Mediterranean area and enhancement of performance of well-established species through nutrition and sustainable practices.

Patents in recent years and the creation of two spin-off companies, in feed manufacture (Sparos, Lda) and in biomedical diagnostics (GenoGla http://www.genogla.com).


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4. ACTIVITIES

4.1 Integrative/multidisciplinary activities during the year of 2010

- Continued contribution to databases of coastal marine and marine ecosystems (M@RBYS) maintained by the Estrutura de Missão para a Extensão da Plataforma Continental (Task Group for the Extension of the Portuguese Continental Shelf); participation in the EMEPC/M@rBis/Selvagens campaign in June 2010.

- Continued partnership with INEGI (Institute of Mechanical Engineering and Industrial Management) for development of hyperbaric chambers involving teams of engineers and marine biologists.

- Continued editions of Marine and Environmental Sciences Doctoral Programme involving CIMAR and CESAM (Universities of Porto, Aveiro and Algarve).

- A new ERASMUS Mundus doctoral programme in Marine Sciences – Mares, Marine Health and Conservation – dealing with the impacts of humans on the natural marine environment, from coastal engineering structures, to sound pollution, biodiversity and resource management. It promotes mobility and multidisciplinarity.

- Collaborations between biologists and chemists to identify new biomolecules and their function: from pheromones in fishes to toxins in cyanobacteria and cytostatic compounds from sponges with potential for pharma industry.

- Collaborations between industry and aquaculture-nutrition groups, and between the latter and molecular biology groups. The goals are to get new species cultured, to promote growth improvements, and, ultimately, to understand growth mechanisms derived from new nutritional formulations.

- Collaborations between biologists and oceanographers for correlating physical oceanographic features with fish and shrimp migration patterns, aiming conservation and sustainable exploitation.

4.2 Outreach activities during the year of 2010

CIMAR manages two centres for environmental interpretation and monitoring through agreements with the municipalities: CMIA-Vila do Conde (www.cmia-viladoconde.net) and CMIA-Matosinhos (www.cmia-matosinhos.net). During 2010, CMIAs displayed 7 temporary exhibitions, organized conferences and performed regular in house and field activities for public of different ages. A Scientific Culture fortnight (11-29 Nov), an intensive program of science outreach for the general public that included 6 conferences, 3 exhibitions, 4 workshops and 1 field trip was also organized. Also, CIMAR scientists manage two public aquariums and fisheries museums: Aguda Littoral Station - ELA (www.fundacao-ela.pt, ca 50000 visitors/year) and River Minho AQUAMuseum (www.cimsoft.pt/aquamuseu, ca 30000 visitors/year). Both stations have a special relevance in what concerns Environmental Education Programs.


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CIMAR regularly participates in the Ciência Viva Programme (http://www.cienciaviva.pt) to contribute to the promotion of a scientific and technological culture among the population and more specifically to encourage the use of experimental methods in science learning, involving both scientific and school communities. In 2010, CIMAR participated in the following activities: Scientists go to School, Science on Holidays for Young People, Biology Activities in the Summer, “Café de Ciência - Science for Biodiversity” (17 March), EXPOMAR exhibition in Olhão (28 April-2 May), “Bioblitz - Looking for marine biodiversity” (4-5 Sept), Science and Technology Week (22-28 Nov), “First encounters with Science” (23-27 Nov) and the European initiative “Reseachers’ Night” (24 Sept).

Since 2006, CIMAR has been organizing the Sea Itinerant University (UIM) (http://www.cimar.org/CIIMAR/en/hz_pas_uim.htm), a program that aims to promote the maritime identity of Portuguese and Spanish students. Courses were organized on land and on board the vessel CREOULA, a 4-masted training ship of the Portuguese Navy, under the theme "Knowledge and adventure".

CIMAR regularly participates in events and exhibitions aimed at encouraging high schools students to engage in scientific careers, such as the “Junior University” (http://universidadejunior.up.pt/), “Science, Education and Innovation” exhibition by the Univ of Porto (25-28 March) and LAB-IT®-The Itinerant Laboratory in Molecular Genetics. The Centre continued the collaboration with the Visionarium - Europarque Science Centre to implement a national project using Daphnia as biological model for experimental teaching of sciences in high schools, and coordinated MOBIDIC - School in the Beach (http://www.estgm.ipb.pt/~projig/mobidic), an outreach program for intertidal biodiversity monitoring in beaches of the North coast. Both CIIMAR and CCMAR received visits from schools throughout the year.

Other activities included the development of a new website for CIIMAR (www.ciimar.up.pt), the release of CIIMAR Newsletter - WAVES, pages in social networks (e.g. Facebook, Twitter, Youtube, Google maps, Vimeo,) and the organization of weekly internal seminars at both centres.

Dissemination was also channeled through the media: publications in non-scientific journals and newspapers (e.g. Alphagalileo, Ciência Hoje, JN, DN, Expresso, Agência Lusa, Visão, National Geographic), participation in TV and radio programs (e.g. SIC, Rádio Renascença, Antena 1).


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5. FUNDING

Origin 2006 2007 2008 2009 2010

LA FCT * 1.092.380,00 2.913.911,00 2.977.162,00 2.788.243,44 2.999.799,00

Units FCT ** 0,00 0,00 0,00 0,00 0,00

Project FCT 570.952,00 827.775,00 2.020.917,77 2.096.417,55 3.086.736,26

Other National 197.458,00 240.018,00 487.715,32 928.168,44 330.877,25

Other International

659.879,00 1.082.379,00 1.200.367,88 1.018.067,73 569.252,44

National Industry

140.634,00 258.989,00 140.135,50 464.280,75 433.581,72

International Industry

0,00 0,00 0,00 88.421,10 49.507,45

Total 2.661.303,00 5.323.072,00 6.826.300,47 7.383.599,01 7.469.754,12

(*) Base + Programmatic; (**) Base + Programmatic of the units before the criation of the LA.


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6. GENERAL INDICATORS

6.1 Composition and training

2006 2007 2008 2009 2010 Total

No. of Researchers Hired (Ciência Programme)

0 0 10 6 0 16

No. of researchers (FTE) 154 199 203 203

Training Masters (Master theses completed)

17 44 57 70 70 188

Training PhDs (PhD theses completed)

23 37 26 24 24 110


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7. RESEARCH LINES (THEMATIC AREAS)

RL1 - Ecology, Biodiversity and Management of Aquatic Ecosystems

7a. General description

1. Designation

Ecology, Biodiversity and Management of Aquatic Ecosystems

2. Principal investigator

Karim Erzini

3. Main scientific domain

Marine Sciences

7b. Objectives and achievements

1. General objectives

The general objectives of RL1 are to study selected aspects of marine geology, oceanography, marine biodiversity and ecosystems, integrating research to provide a sound scientific basis for assessment, management, and policy related with the marine ecosystems. Within this framework, the objectives include the following:

- Promote the geological research of the oceans and coastal regions, the paleoceanography, and the processes related to the occurrence of marine mineral resources;

- Determine the composition, abundance and distribution of the national marine biodiversity;

- Determine the impacts of climate change and local human action by monitoring and studying coastal and estuarine ecosystem functioning, and long-term changes in marine communities and habitats;

- Study the genetics, population biology, ecology and recruitment of ecologically and commercially important species;

- Investigate the physiological adaptations of migratory fishes, to different salinity and hydrostatic pressure;

- Participate in International networks on marine biodiversity;


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- Train young scientists and to disseminate scientific knowledge.

2. Main achievements

Ecotoxicology and pollution

- Development of ecotoxicity lab bioassay and determination the physiological impact of toxicant exposure and environmental changes through the measurement of biomarkers. Study of effects of PAHs, pesticides, metals and pharmaceuticals on several marine and freshwater species of temperate and tropical areas, including mechanisms of toxicity and detoxification.

Genetics and phylogeography

- Contributions to understanding marine evolutionary ecology and biogeography of a range of species, from sea grasses to mid-Atlantic ridge vent sea shrimps, sea cucumbers and fishes.

Biology, ecology and conservation

- Contributions to the understanding of the life cycles and population dynamics of a wide range of marine species. Evaluation of the effects of marine protected areas.

Culture and economic valorisation

- Important advances in the culture and economic valorisation of species such as the red alga, cuttlefish and sea horses.

Coastal, estuarine and lagoon processes

- Assessment of the ecological status of transitional waters and development of tools towards the implementation of the WFD. Development of hydrodynamic models. Assessment of coastal dynamics, vulnerability, mapping and risk assessment. Recruitment studies of estuarine and lagoon species.

Global changes

- Improved knowledge of effects of climate events, ocean acidification, temperature, precipitation and introduced/invasive species. Creation of database for studying large scale patterns and long-term changes.

Technical developments and capacity building

- Enlargement of a computation cluster, now providing a platform for genome assembly phylogenetic analyses, BLAST analyses, transcriptome assembly and expression analyses. Completion of a new laboratory equipped with state-of-the-art instruments for microbial metagenomics / molecular ecology research.


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Biodiversity

- Discovery and description of new species, mapping of biodiversity and identification of essential habitats in coastal waters, contributions and creation of data bases.

Networks

- Participation in several EU Networks (e.g. COST ACTION, LargeNet project of MarBEF, MARS, EMBRC, EPBRS) and other international collaborative projects.

Research training

- Training at the undergraduate and graduate levels (MSc and PhD programs), participation in the Erasmus Mundus MSc in Marine Biodiversity and Conservation and PhD MARES.

Dissemination & Outreach

- Activities to foster dialogue between scientists and society.

7c. Research lines output

1. Collaborative publications in peer review journals

Examples of collaborative publications:

Azevedo, IC, Bordalo, AA, Duarte, PM. 2010. Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersal in the Douro estuary (Portugal). Water Research 44: 3133-3146.

Azevedo, IC, Duarte, PM, Bordalo, AA. 2010. Temporal and Spatial variability of phytoplankton photosynthetic characteristics in a southern European estuary (Douro, Portugal). Marine Ecology Progress Series 412: 29-44.

Bertocci, I et al. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116.

Campos, J et al. 2010. Fluctuations of brown shrimp Crangon crangon abundance in the western Dutch Wadden Sea. Marine Ecology Progress Series 405: 203-219.

Carvalho, PN et al. 2010. Ability of salt marsh plants for TBT remediation in sediments. Environmental Science and Pollution Research 17: 1279-1286.

Costa-Dias, S, Sousa, R, Antunes, C. 2010. Ecological quality assessment of the lower Lima estuary. Marine Pollution Bulletin 61: 234-239.

Delgado, C, Canário, AVM, Dellinger, T. 2010. Sex ratios of loggerhead sea turtles Caretta caretta during the juvenile pelagic stage. Marine Biology 157: 979-990.


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Ferreira, F et al. 2010. Sexing blennies using genital papilla morphology or anogenital distance. Journal of Fish Biology 77: 1432-1438.

Freitas, V et al. 2010. Temperature tolerance and energetics: a dynamic energy budget-based comparison of North Atlantic marine species. Philosophical Transactions of the Royal Society B 365: 3553-3565.

Morais, P et al. 2010. Diversity of anchovy migration patterns in an European temperate estuary and in its adjacent coastal area: Implications for fishery management. Journal of Sea Research 64: 295-303.

Mucha, AP et al. 2010. LMWOA exudation by salt marsh plants: Natural variation and response to Cu contamination. Estuarine, Coastal and Shelf Science 88: 63-70.

Vandepitte, L et al. 2010. Data integration for European marine biodiversity research: creating a database on benthos and plankton to study large-scale patterns and long-term changes. Hydrobiologia 644: 1-13.

2. Collaborative other publications

Examples of collaborative other publications:

Pereira, R, Yarish, C. 2010. The role of Porphyra in sustainable culture systems: Physiology and applications. In: Israel, A, Einav, R (Eds). Role of Seaweeds in a Globally Changing Environment, Springer Publishers, pp. 339-353.

3. PhD theses completed (Co-supervision or clearly multidisciplinary projects)

From the 9 PhD theses completed in 2010, 5 were in collaboration with national institutions, 3 with international institutions.

Azevedo, Alberto Carlos de Oliveira Antunes. 2010. Sistema integrado de modelação para apoio e mitigação de acidentes de hidrocarbonetos em estuários e orla costeira. PhD thesis, LNEC/University of Lisbon. Supervisors: Anabela Pacheco de Oliveira (LNEC), José CPB Teixeira da Silva.

Bartilotti, Cátia Alexandra Vieira. 2010. Decapod crustacean larvae dynamics in the west continental coast of Portugal: the region adjacent to Aveiro coastal lagoon as model. PhD thesis in Marine Ecology, University of Algarve. Supervisor: Antonina dos Santos (IPIMAR), Co-supervisor: Margarida Castro (CIMAR-FBC).

Faria, Ana. 2010. Ontogeny of behavioural abilities in temperate reef fish larvae. PhD thesis, University of Algarve. Supervisors: Emanuel Gonçalves (ISPA), Maria Alexandra Chícharo (CIMAR-ECOREACH).

Gonçalves, Hernâni. 2010. Automatic registration of satellite images. PhD thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: José A Gonçalves (CIMAR-OCD), Co-supervisor: Luís Corte-Real (INESC Porto, Faculty of Engineering, University of Porto).


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León Cisneros, Karla. 2010. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto (CIMAR-AIR), Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).

Marçalo, Ana Luisa Barreto 2010. Avaliação de stress em sardinha (Sardina pilchardus) durante a pesca do cerco Universidade do Algarve Supervisors: Karim Erzini (CIMAR-FBC), Yorgos Stratoudakis (IPIMAR).

Pereira, A. 2010. Desenvolvimento de um sistema integrado de modelação, calibração e gestão de ecossistemas costeiros, com recurso a agentes inteligentes. PhD in Electronic Engineering and Computers, Faculty of Engineering, University of Porto. Supervisor: Pedro Duarte (CIMAR-Biodiversity of Aquatic Ecosystems).

Rosas Alquicira, Edgar Francisco. 2010. Filogeografia de algas Corallinales (Rhodophyta) das Califórnias. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto (CIMAR-AIR), Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).

Sousa, Joaquim, 2010. Integration of DInSAR and DGPS for location and characterization of active faults. Supervisor: Luísa Bastos (CIMAR-OCD), Co-supervisor: Ramon Hanssen (Delft University of Technology, The Netherlands).


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RL2 - Environmental Chemistry and Toxicology


1. Designation

Environmental Chemistry and Toxicology


Lúcia Maria das Candeias Guilhermino


Environment



The central objective of RL2 is to investigate the effects of natural and anthropogenic stressors on biodiversity and functioning of ecosystems using a molecular to ecosystem level approach. Special attention has been given to long-term alterations induced on marine ecosystems by global stressors (pollution, invasive species and climate changes). Specifically, we intend to:

- Study interactions of stressors with molecular targets to investigate mechanisms of toxicity and defenses

- Compare responses among species and establish phylogeographic patterns of evolution;

- Investigate reflexes of molecular interactions at higher levels of biological organization;

- Develop new methods for human and ecological risk assessment;

- Study the functioning of ecosystems under stress in a climate changing world;

- Monitoring water quality and developing strategies to improve it;

- Develop methods for conservation, bioremediation and restoration, contributing to environmental sustainability.


- More than 65 papers in international journals and 70 presentations in meetings by the 5 core labs.

- Knowledge on the role of vascular plants in conditioning the environment & plant capability for rhizoremediation.


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- Development and optimization of analytical techniques to measure environmental contaminants and cyanobacteria toxins, and their environmental degradation.

- Characterization and allelopathy of compounds produced by cyanobacteria.

- Knowledge on the phylogeny of glutathione S-transferases and phosphoprotein phosphatases enzymes.

- Knowledge on the mechanisms of toxicity, detoxication of contaminants (e.g. PAHs, metals, pharmaceuticals, Hazardous & Noxious Substances, oils, xenostrogens) and cyanotoxins.

- Demonstration that the nuclear receptor RXR is a key player in imposex induction by the androgenic chemical tributyltin.

- Cloning and sequencing of several ABC transporter genes, and others, in model species.

- Insights of modeling of PPARα and other genes involved on lipid metabolism in fish.

- Development of software tools to perform evolutionary analyses on gene/protein sequences

- Knowledge on the biological and ecological effects of contaminants.

- Validation of several biomarkers and organisms as sentinel species for use in monitoring and environmental risk assessment in the marine environment.

- Relationships between several biomarkers, behaviour and population parameters.

- Derivation of PNECs for marine species.

- Validation of integrated approaches (ecology, ecotoxicology & chemistry) for monitoring in marine environments.

- Knowledge on the impact of extreme events (e.g. heatwaves) on the competition between native and invasive aquatic species, population and communities resistance and resilience, and ecosystem functioning.

- Impacts of climate changes and pollution on biogeochemical cycles.

- Ecological, ecotoxicological and/or toxicological knowledge on tropical and Antarctic ecosystems.

- Scientific support to regulations and policies .


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Azevedo, IC, Bordalo, AA, Duarte, PM. 2010. Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersal in the Douro estuary (Portugal). Water Research 44: 3133-3146.

Carvalho, PN et al. 2010. Ability of salt marsh plants for TBT remediation in sediments. Environmental Science and Pollution Research 17: 1279-1286.

Costa-Dias, S et al. 2010. Factors influencing epibenthic assemblages in the Minho estuary (NW Iberian Peninsula). Marine Pollution Bulletin 61: 240-246.

Costa-Dias, S, Sousa, R, Antunes, C. 2010. Ecological quality assessment of the lower Lima estuary. Marine Pollution Bulletin 61: 234-239.

El Ghazali, I et al. 2010. Effects of the microcystin profile of a bloom on the toxicity and toxin accumulation in common carp (Cyprinus carpio L.). larvae. Journal of Fish Biology 76:1415-1430.

El Ghazali, I et al. 2010. Effect of different microcystin profiles on the toxins bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety 73:762-770.

Ferreira, F et al. 2010. Sexing blennies using genital papilla morphology or anogenital distance. Journal of Fish Biology 77: 1432-1438.

Mucha, AP et al. 2010. LMWOA exudation by salt marsh plants: Natural variation and response to Cu contamination. Estuarine, Coastal and Shelf Science 88: 63-70.

Nogueira, I et al. 2010. Toxic effects of domoic acid in the sea bream Sparus aurata. Marine Drugs 8: 2721-2732.

Silva, P et al. 2010. Expression of the myosin light chains 1,2 and 3 in the muscle of blackspot seabream, Pagellus bogaraveo, during development. Fish Physiology and Biochemistry 36: 1125-1132.

Stoichev, T et al. 2010. Fate and effects of nonylphenol in the presence of the cyanobacterium Microcystis aeruginosa. Chemistry and Ecology 26: 395-399.

In press

Lima, D et al. Aquatic Toxicology, dói: 10.1016/j.aquatox.2010.09.022.

Mucha, AP et al. International Biodeterioration & Biodegradation, doi: 10.1016/j.ibiod.2010.12.005.

Ribeiro, H et al. Biodegradation, doi: 10.1007/s10532-010-9446-9.


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Sarria, MP et al. Environment International, doi:10.1016/j.envint.2010.11.004.

Silva, P, et al. Aquaculture Research, doi: 10.1111/j.1365-2109.2010.02627.x.



Wilson, JM, Castro, LFC. 2010. Morphological diversity of the gastrointestinal tract in fishes. In: Grosell, M, Farrell, AP, Brauner, CJ (Eds). The multifunctional gut of fish – Volume 30, Elsevier Inc., pp. 1-55. ISBN: 978-0-12-374982-6.

In press

Santos, MM, Reis-Henriques, MA, Castro, LFC (in press). Lipid homeostasis perturbation by organotins: effects on vertebrates and invertebrates. In: Pagliarani, A., Trombetti, F, Ventrella, V (Eds). Biochemical and biological effects of organotins, Chapter 6. Beham Science Publishers, Ltd.


From the 4 PhD theses completed in 2010, 2 were in collaboration with national institutions.

Couto, Maria Nazaré PFS. 2010. Investigation of physical, chemical and biological factors implicated in the biological remediation of nonvolatile petroleum hydrocarbons. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto (CIMAR-CSB)

Leão, Pedro. 2010. Allelopathic properties of cyanobacteria isolated from Portuguese freshwaters. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos (CIMAR-LEGE)

Lima, Inês de Marrazes. 2010. Ecotoxicological effects of petrochemical products on natural populations of Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Lúcia Guilhermino (CIMAR-ECOTOX), Co-supervisor: Amadeu MVM Soares (CESAM, University of Aveiro).

Ribeiro, Cláudia Maria Rosa. 2010. Chemical identification and monitoring of natural and xenoestrogens compounds in the estuaries of the estuaries of the Portuguese rivers Douro, Mondego and Sado – Insights on their disruption potential by in vitro exposures of testis from the model organism Nile Tilapia (Oreochromis niloticus). PhD Thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisor: Maria João Rocha (CIMAR-LECEMA), Co-supervisors: Eduardo Rocha (CIMAR-LECEMA), Maria Elisabeth Tiritan (Faculty of Pharmacy, University of Porto).


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RL3 - Biology and Marine Biotechnologies


1. Designation

Biology and Marine Biotechnologies


Deborah Mary Power


Biological Sciences



The Biology and Marine Biotechnology RL saw the inclusion of 2 new groups in 2010 to consolidate its broad portfolio of basic research. The central pillars are the same as previous years:

- To extend fundamental knowledge about marine organisms in a changing environment at a molecular (omics) to whole organism (systems biology) level and across the evolutionary scale;

- To establish how natural and man-made environmental challenges and pathogens impact organism function and survival;

- To reinforce the drive to promote the identification of potential “spin-offs” arising from basic biology and stimulate pilot studies and links with industry (eg. “near market” solutions, such as, genotyping for aquaculture species, diagnostic tests in pathology);

- There is continued commitment to disseminate knowledge in high impact peer-review journals and at International conferences and provide high quality training for a new generation of scientists and contribute to generate a “knowledge-based society”. Translation of research activities and communication to other stakeholders (scientists, policy makers, industry, teachers and the public) is a priority.


This RL includes 13 RG and this section presents only major achievements perceived as having a significant impact at a national or international level.

- Application of high throughput methods of transcriptome analysis (microarray, SAGE, 454 sequencing) for teleosts subject to physiological challenges, such as, hydrostatic


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pressure, cortisol and divergent somatic growth. Transcriptome analysis (454 seqeuncing) of the Antarctic bivalve (Laternula elliptica) for discovery of novel genes.

- Collaboration with colleagues from Bangor Univ in the discovery of unsuspected diversity of metazoan in benthic habitats. The methodology used is proof of concept of allying “new generation sequencing” to PCR and bioinformatics.

- Accomplished with colleagues from the Max Plank Institute of Molecular Biology a draft sequence of the European sea bass genome, a basic resource for the scientific community and aquaculture industry.

- Draft regulatory network of genes involved in ion homeostasis in enterobacteria (E. coli) and completion of Perkinsus genome.

- Identification and description of pathogens and parasites of fish of economic interest, such as turbot and blackspot seabream together with pathogens from invertebrates.

- Consolidation of the research area of chemical biology and marine biotechnology. Screening methods established for bioactive compounds in marine photosynthetic and isolation of bioactive secondary metabolites from fungus.

- Refinement of a bio-refinery strategy and isolation of several strains, of microalgae with a very promising lipid profile suitable for the production of biodiesel.

- Consolidation of a chemical survey and tools for analysis of xenoestrogenic endocrine disrupting compounds (EDCs) in marine fish and bivalves in Portugal water ways. Identification of peroxisome receptors (PPARs) as a novel estradiol target.

- Spin-offs; design and synthesis of endoperoxide-based antimalarials and falcipain inhibitors; gammacarboxyglutamate-rich protein, methods and assays for its detection and quantification; method to deduce the three dimensional structure of a protein from its amino acid sequence.

- Consolidation of links and collaborations with Agência Ciência Viva and Centro de Ciência Viva to communicate science to schools and the general public.




Alves, RN, Cordeiro, O, Silva, TS, Richard, N, de Vareilles, M, Marino, G, Di Marco, P, Rodrigues, PM, Conceição, LEC. 2010. Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics. Aquaculture 299: 57-66.


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Conceicão, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2010. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry 36: 1-16.

Conceicao, N, Cox, CJ, Simões, B, Viegas, M, Cancela, ML. 2010. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology 26(2): 245-250.

Delgado, C, Canário, AVM, Dellinger, T. 2010. Sex ratios of loggerhead sea turtles Caretta caretta during the juvenile pelagic stage. Marine Biology 157: 979-990.

Louro, B, Passos, ALS, Souche, E, Tsigenopoulos, C, Beck, A, Bonhomme, F, Cancela, L, Cerdà, J, Clark, MS, Kotoulas, G, Lubzens, E, Planas, J, Volckaert, FAM, Reinhardt, R, Canario, AVM. 2010. Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: characterization, tissue specific expression and gene markers. Marine Genomics 3(3-4): 179-191.

Nogueira, I, Lobo da Cunha, AL, Afonso, A, Rivera, S, Azevedo, J, Monteiro, R, Cervantes, R, Gago-Martinez, A, Vasconcelos, V. 2010. Toxic effects of domoic acid in the sea bream Sparus aurata. Marine Drugs 8: 2721-2732.

Rosa, J, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2010. Serum-related stimulation of the proliferation and mineralization of seabream bone-derived cells. Journal of Applied Ichthyology 26: 245-250.

Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the myosin light chains 1,2 and 3 in the muscle of blackspot seabream during development. Fish Physiology and Biochemistry 36: 1125-1132.

Viegas, CS, Conceição, N, Fazenda, C, Simes, DC, Cancela, ML. 2010. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon (Acipenser naccarii). Journal of Applied Ichthyology 26: 214-218.

In press

Lima, D et al. Aquatic Toxicology, doi: 10.1016/j.aquatox.2010.09.022.

Silva, P et al. Aquaculture Research, doi: 10.1111/j.1365-2109.2010.02627.x.


Examples of other collaborative publications:

Cancela, ML, Bargelloni, L, Boudry, P, Boulo, V, Dias, J, Huvet, A, Laizé, V, Lapègue, S, Leite, R, Mira, S, Nielsen, EE, Planas, JV, Roher, N, Sarropoulou, E, Volckaert, FAM. 2010. Genomic Approaches in Aquaculture and Fisheries. In: Cock, JM, Viard, F, Tessamar-Raible, K, Boyen, C (Eds). Introduction to Marine Genomics, Springer-Verlag, Heidelberg, pp. 213-286. ISBN 978-90-481-8616-7.


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Ozorio, ROA, Hubbard, PC, Barata, EN, Valente, LMP, Canário, AVM. 2010. Olfactory Sensitivity to Amino Acids in the Blackspot Seabream (Pagellus Bogaraveo, Brünnich 1768): How Effective is the Electro-olfactogram in Seawater?. Aquaculture Europe 2010, 5-10 October, Porto, Portugal. European Aquaculture Society, pp. 946-947.

Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML, Simes, DC. 2010. Gla-rich protein is a novel vitamin K-dependent protein present in serum that accumulates at sites of pathological calcifications. Journal Club, Kidney International 77: 265-266.

Wilson, JM, Castro, LFC. 2010. Morphological diversity of the gastrointestinal tract in fishes. In: Grosell, M, Farrell, AP, Brauner, CJ (Eds). The multifunctional gut of fish – Volume 30, Elsevier Inc., pp. 1-55. ISBN: 978-0-12-374982-6.

In press

Santos, MM, Reis-Henriques, MA, Castro, LFC (in press). Lipid homeostasis perturbation by organotins: effects on vertebrates and invertebrates. In: Pagliarani, A., Trombetti, F, Ventrella, V (Eds). Biochemical and biological effects of organotins, Chapter 6. Beham Science Publishers, Ltd.


From the 6 PhD theses completed in 2010, 1 was in collaboration with national institutions, and 2 with RG within the RL.

Francisco, Claire Juliana. 2010. Parasitas do mexilhão Mytilus galloprovincialis Lamark, 1819 (BIVALVIA: MYTILIDAE) com relevância para Trematoda: ciclo de vida, características morfológicas e moleculares. PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Maria João Santos (CIMAR-Pathology).

Guerreiro, Liliana Isabel Tomé dos Anjos. 2010. Biochemical and biological characteristics of novel piscine forms of parathyroid hormone and parathyroid hormone related peptide: a proteomic approach. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power (CIMAR-CME).

Martins, Florbela Alexandra Silva Vieira. 2010. Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power (CIMAR-CME).

Neves, Mónica. 2010. Bio-removal of toxic metals by metal resistant anaerobic bacteria: molecular characterization and performance studies. PhD thesis in Environmental Biotechnology, University of Algarve. Supervisor: Maria Clara Costa (CIMAR-ET), Co-supervisor: Raul Barros (University of Algarve).

Ribeiro, Cláudia Maria Rosa. 2010. Chemical identification and monitoring of natural and xenoestrogens compounds in the estuaries of the estuaries of the Portuguese rivers Douro, Mondego and Sado – Insights on their disruption potential by in vitro exposures of testis from the model organism Nile Tilapia (Oreochromis niloticus). PhD Thesis in Biomedical Sciences,


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ICBAS, University of Porto. Supervisor: Maria João Rocha (CIMAR-LECEMA), Co-supervisors: Eduardo Rocha (CIMAR-LECEMA), Maria Elisabeth Tiritan (Faculty of Pharmacy, University of Porto).

Viegas, Carla. 2010. Identification and characterization of a new calcium binding protein from sturgeon, Gla rich protein or GRP. PhD thesis in Biochemistry – Cellular and Molecular Biology, University of Algarve. Supervisor: M Leonor Cancela (CIMAR-EDGE), Co-supervisor: Dina C Simes (CIMAR-FBP).


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RL4 - Aquaculture


1. Designation

Aquaculture


Maria Teresa Dinis


Marine Sciences



The objective of RL is to promote the generation of high quality scientific knowledge through fundamental and applied research, to tackle some of the sustainability challenges facing the aquaculture industry.

- Strategic research areas were defined and clearly consolidated.

- Biological assessment of the cultivation potential of new aquaculture species, for the establishment of sustainable culture practices to facilitate the industrialization process in areas of broodstock management, larval rearing, nutrition, growth and flesh quality of new species for Mediterranean aquaculture.

- Fish Nutrition, which fostered a better understanding of the nutritional modulation of intermediary metabolism, oxidative stress damage, nutritional requirements, utilization of alternative feedstuffs to fish meal and oil and optimal feed formulations for enhanced performance, improved quality of fish and lower environmental impact.

- Fish Immunology & Health aiming a better understanding of host/pathogens interaction mechanisms and the establishment of immunostimulation strategies to enhance stress resistance and welfare of farmed fish.


The collaboration between the RG of RL4 was reinforced. The major achievement of the RL is its contribution through fundamental knowledge and practical collaboration with the industry to the establishment of intensive and semi-intensive farming systems for new species in the Mediterranean area and enhancement of performance of well established species through nutrition and sustainable practices.


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Main scientific achievements:

- Development of practical diets with low levels of marine-derived proteins for Senegalese sole, gilthead seabream, white seabream.

- Optimization of nutrient requirements, dietary formulations and feeding practices for blackspot seabream and reinforcement of research on the nutritional requirements of Diplodus sp. aiming a comparative evaluation among species and establishing the potential for aquaculture of these promising omnivorous species.

- Evaluation of different feed restriction protocols on growth performance and compensatory growth of turbot under commercial rearing conditions. The effect of different feeding regimes in growth, feed utilization and N excretion of Senegalese sole was evaluated. This information is of immediate practical application for the management of feeding in turbot and sole aquaculture.

- Evaluation of blood plasma metabolites and free amino acid levels in Senegalese sole after feed deprivation indicated a high usage of body protein for as energy substrates, a higher ammonia detoxification activity and active liver gluconeogenic processes.

- Development of immunostimulation strategies to enhance stress resistance and immune response in Senegalese sole.

- Proposition of a set of chronic stress molecular markers based on liver proteome expression, which includes proteins involved in eg. protein metabolism, carbohydrate metabolism and energy homeostasis.

- Pursue the valorization of aquaculture products potential following 2 vectors: benefits evaluation for human health of the incorporation of conjugated linoleic acid in the diets and evaluating sensory, physical, microbiological characterization of freshness in products.

- Understanding the environmental and epigenetic factors that regulate muscle differentiation to optimize growth and meat quality.




Alves, RN, Cordeiro, O, Silva, TS, Richard, N, de Vareilles, M, Marino, G, Di Marco, P, Rodrigues, PM, Conceição, LEC. 2010. Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics. Aquaculture 299: 57-66.


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Bertocci, I, Arenas, F, Matias, M, Vaselli, S, Araújo, R, Abreu, H, Pereira, R, Vieira, R, Sousa-Pinto, I. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116.

Conceicão, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2010. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry 36: 1-16.

Dias, J, Yúfera, M, Valente, LMP, Rema, P. 2010. Feed transit and apparent protein, phosphorus and energy digestibility of practical feed ingredients by Senegalese sole (Solea senegalensis). Aquaculture 302: 94-99.

El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, F, Vasconcelos, V, Oudra, B. 2010. Effects of the microcystin profile of a bloom on the toxicity and toxin accumulation in common carp (Cyprinus carpio L.). larvae. Journal of Fish Biology 76:1415-1430.

El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, del Campo, FF, Oudra, B, Vasconcelos, V. 2010. Effect of different microcystin profiles on the toxins bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety 73:762-770.

Rosa, J, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2010. Serum-related stimulation of the proliferation and mineralization of seabream bone-derived cells. Journal of Applied Ichthyology 26: 245-250.

Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the myosin light chains 1,2 and 3 in the muscle of blackspot seabream, Pagellus bogaraveo, during development. Fish Physiology and Biochemistry 36: 1125-1132.

In press

Abreu, MH, Pereira, R, Yarish, C, Buschmann, AH, Sousa-Pinto, I. (in press). Aquaculture, doi: 10.1016/j.aquaculture.2010.12.036.

Silva, P, Valente, LMP, Olmedo, M, Álvarez-Blázquez, B, Galante, MH, Monteiro, RAF, Rocha, E. (in press). Aquaculture Research, doi: 10.1111/j.1365-2109.2010.02627.x.



Cancela, ML, Bargelloni, L, Boudry, P, Boulo, V, Dias, J, Huvet, A, Laizé, V, Lapègue, S, Leite, R, Mira, S, Nielsen, EE, Planas, JV, Roher, N, Sarropoulou, E, Volckaert, FAM. 2010. Genomic Approaches in Aquaculture and Fisheries. In: Cock, JM, Viard, F, Tessamar-Raible, K, Boyen, C (Eds). Introduction to Marine Genomics, Springer-Verlag, Heidelberg, pp. 213-286. ISBN 978-90-481-8616-7.

Ozorio, ROA, Hubbard, PC, Barata, EN, Valente, LMP, Canário, AVM. 2010. Olfactory Sensitivity to Amino Acids in the Blackspot Seabream (Pagellus Bogaraveo, Brünnich 1768): How Effective is the Electro-olfactogram in Seawater?. Aquaculture Europe 2010, 5-10 October, Porto, Portugal. European Aquaculture Society, pp. 946-947.


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Pereira, R, Yarish, C. 2010. The role of Porphyra in sustainable culture systems: Physiology and applications. In: Israel, A, Einav, R (Eds). Role of Seaweeds in a Globally Changing Environment, Springer Publishers, pp. 339-353.

Quental-Ferreira, H, Ramalho Ribeiro, A, Dias, J, Yúfera, M, Arias, AM, Falcão, M, Serpa, D, Aires, T, Pousão-Ferreira, P, Cunha, ME, Valente, LMP, Dinis, MT; Conceição, L. 2010. Sustainable semi-intensive polyculture of seabream and sole in earthern ponds. Aquaculture Europe 35(3): 17-21.

Vaz-Pires, P, Escórcio, C, Soares, S, Ramalho, A, Matos, E, Dias, J. 2010. Optimising fish slaughtering procedures for semi-intensive aquaculture. Aquaculture Europe 35(3): 26-28.

Vaz-Pires, P, Ramalho, A, Jesus, A, Soares, F, Conceição, L, Dinis, MT, Ribeiro, L, Pousão-Ferreira, P, Cunha, ME, Quental-Ferreira, H, Yúfera, M, Marino, G, Boglione, C, Cataudella, S, Hussenot, J, Begout, M-L, Makridis, P, Buard, E, Blachier, P. 2010. A joint European certification system for sustainable non-intensive aquaculture: a proposal from the SEACASE Project. Aquaculture Europe 35(2): 17-22.


From the 4 PhD theses completed in 2010, 2 were in collaboration with national institutions, 3 with international institutions, and 1 with RG within the RL.

Barrento, Sara. 2010. Nutritional quality and physiological responses to transport and storage of live crustaceans traded in Portugal. PhD thesis in Animal Science, ICBAS, University of Porto. Supervisor: Maria Leonor Nunes (IPIMAR), Co-supervisor: Paulo Vaz-Pires (CIMAR-LANUCE).

Ribeiro, Ana Rita de Araújo. 2010. Thyroid status in senegalese sole (Solea senegalensis) larvae – effects of iodine and selenium supplementation. PhD thesis in Aquaculture, University of Algarve. Supervisors: Maria Teresa Dinis (CIMAR-Aquagroup), Laura Ribeiro (IPIMAR), Mari Moren (NIFES, Norway).

Silva, Amélia Cláudia Figueiredo. 2010. Nutritional regulation of lipid deposition in blackspot seabream (Pagellus bogaraveo). PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa MP Valente (CIMAR-LANUCE), Co-supervisoror: Geneviéve Corraze (INRA, France).

Silva, Joana Margarida Guimarães. 2010. Use of alternative protein sources in diets for senegalese sole (Senegalese sole Kaup, 1858) juveniles. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa MP Valente (CIMAR-LANUCE)., Co-supervisoros: Marite Espe (NIFES, Norway), Luís Conceição (CIMAR-Aquagroup).


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8. RESEARCH GROUPS

1. Research group

Principal Investigator Name of the Research Groups

Maria Teresa Dinis Aquaculture Research Group (Aquagroup)

Ana Isabel de Melo Azevedo Neto Aquatic Insular Research (AIR)

Maria Ester Tavares Alvares Serrao Biogeographical Ecology and Evolution (BEE)

Maria Leonor Nunes Ribeiro Cruzeiro Biophysics

Manuel Aureliano Pereira Martins Alves Cellular and Inorganic Biochemistry (CIB)

Eduardo Jorge Sousa Rocha Cellular, Molecular and Analytical Studies (LECEMA)

Maria Teresa Sá Dias de Vasconcelos Chemical Speciation and Bioavailability (CS&B)

Anake Kijjoa Chemistry and Biological Activity of Marine Natural Products

Isabel Maria Trigueiros Sousa Pinto Machado Coastal Biodiversity

Adelino Vicente Mendonca Canario Comparative Molecular Endocrinology (CME)

Luis Manuel Zambujal Chicharo Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)

João José Oliveira Dias Coimbra Ecophysiology

Lúcia Maria das Candeias Guilhermino Ecotoxicology and Ecology (ECOTOX)

Vitor Manuel Oliveira Vasconcelos Ecotoxicology, Genomics and Evolution (LEGE)

Maria Clara Semedo da Silva Costa Environmental Technologies


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Maria Armanda Reis Henriques Environmental Toxicology (LETOX)

Maria Leonor Quintais Cancela Fonseca Evolution, Development and Gene Expression (EDGE)

Aires Manuel Pereira Oliva Teles Fish Nutrition

José Pedro de Andrade e Silva Andrade Fisheries Biology and Hydroecology Research Group (FBHRG)

Karim Erzini Fisheries, Biodiversity and Conservation

Dina Cristina Fernandes Rodrigues da Costa Simes

Functional Biochemistry and Proteomics

João José Oliveira Dias Coimbra Geology and Paleoceanography

Adriano Agostinho Donas Bôto Bordalo e Sá Hydrobiology

João Carlos Serafim Varela Marine Biotechnologies (MarBiotech)

Elsa Maria Branco Froufe Andrade Marine Community Ecology and Evolution (LMCEE)

Rui Orlando Pimenta Santos Marine Plant Ecology (ALGAE)

Rodrigo da Silva Costa Microbial Ecology and Evolution

Fernando Francisco Machado Veloso Gomes Modelling and Coastal Management

Luísa Maria Pinheiro Valente Nutrition, Growth and Quality of Fish (LANUCE)

Maria Luisa Machado Cerqueira Bastos Oceanic and Coastal Dynamics

Jorge Guimaraes da Costa Eiras Pathology

Cymon John Cox Plant Systematics and Bioinformatics

Maria Lurdes Santos Cristiano Synthesis and Organic Reactivity


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Aquaculture Research Group (Aquagroup)

8a. Group description

1. Group name / denomination

Aquaculture research group (Aquagroup)


Maria Teresa Dinis

3. Location of group

CCMAR - Centro de Ciências do Mar, Faro

8b. Funding, sources, dates

In 2010, the Aquagroup was involved in 13 research projects, in 7 of them with a coordination role. The majority of projects (8) were funded through FCT, while 5 were undertaken by European funding (FP6, FP7 and national funding schemes for industrial collaborations). The total funding (full duration of projects) for Aquagroup encompassed 1.57 Millions EUR (including overheads).

Funds spent in 2010:

- FCT pluriannual funding

- National funding

31.756 EUR, FCT, PTDC/MAR/67017/2006, EFAR

43.879 EUR, FCT, PTDC/MAR/71685/2006, HYDRAA

70.541 EUR, FCT, PTDC/MAR/70855/2006, FATTYBONE

59.833 EUR, FCT, PTDC/MAR/70858/2006, TEXBREAM

77.907 EUR, FCT, PTDC/MAR/64533/2006, CRYOSPERM

26.723 EUR, FCT, PTDC/CVT/102481/2008, ULTRAFISH

- International funding

6.244 EUR, European Commission, FP6, Contract Nr. 044483, SEACASE

16.228 EUR, European Commission, FP7-SME-2008-1-232305, PROSPAWN


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8c. Objectives and achievements

1. Objectives

The mission of the Aquaculture Research Group is to generate scientific knowledge, through fundamental and applied research, to tackle some of the evolving sustainability challenges facing the aquaculture industry. The team assembles a range of competencies that allow a multidisciplinary approach of several research themes:

- Nutrition and metabolism in marine fish and cuttlefish: studies on the role of amino acids, fatty acids, glucose and vitamins in digestive efficiency, intermediary metabolism, immune competence and skeletal deformities (HYDRAA, FATTYBONE, EFARFish, ULTRAFISH, MICALA, GREENDIETS, SPECIAL K, SEPIAMETA). Besides classical zootechnical and biochemical assessment criteria, most projects integrate also functional genomics, proteomics, tracer studies and metabolic modeling approaches.

- Broodstock Management of new species (Senegalese sole, dusky grouper) with a strong emphasis on nutrition and welfare (ECOAQUA, PROSPAWN) and the improvement of semen cryopreservation success in various farmed species (seabass, seabream and turbot) (CRYOSPERM) through the addition of antioxidants to reduced oxidative stress.

- Quality and Safety of Fish: proteome analysis was associated with traditional biochemical criteria to study the role of nutritional factors and farming practices as modulators of flesh softening and aroma in farmed fish (TEXBREAM, SEACASE).

- Production Systems: has tackled aspects related to the estimation of optimal environmental conditions for natural spawning of sole (PROSPAWN) and the development of effective tools for the assessment of competitiveness and sustainability of extensive and semi-intensive aquaculture production in Southern Europe (SEACASE).

- Fish Welfare: addressed issues as the dietary modulation of the immune status in fish (PhD project) and stress response (Post-doc project) and the definition of operational welfare indicators in broodstock fish (ECOAQUA, PROSPAWN).


Societal

- Through its leading role in the SEACASE project, the Aquagroup has reinforced its dialogue with the Portuguese semi-intensive fish farming industry, creating a strong momentum in its strategy “from lab to farm”, for instance with its activities in the development of eco-friendly feeds for semi-intensive fish farming. The societal relevance of this project is reflected in several radio and newspapers interviews, and also a national TV interview.

Scientific


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- Development of practical diets with low levels of marine-derived proteins for sole and seabream.

- Differential Scanning Calorimetry analysis is a fast and low-cost technique that proved reliable in discerning seabream subjected to different storage conditions.

- Taurine transporter gene in sole was cloned and its expression suggests the existence of an enterohepatic recycling pathway for taurine in flatfish.

- Coping styles were identified in sole juveniles, through the study of individual differences in oxygen consumption under different physiological conditions.

- Cortisol response to air exposure in sole post-larvae is affected by ARA to EPA ratio.

- A set of stress markers was proposed based on liver proteome expression, which includes proteins involved in e.g., protein metabolism, carbohydrate metabolism and energy homeostasis.

- Feed deprivation was shown to affect blood plasma metabolites and free amino acid levels in sole, indicating a high usage of body protein as energy substrates.

- The lipid composition of seabass spermatozoa membranes could be used as indicators of sperm freezability.

- In seabass sperm cryopreservation, the addition of antioxidants to the freezing media improved sperm motility, which is one of the impaired parameters during cryopreservation.

- Sole exposition to spring simulated conditions during autumn, modulated the melatonin rhythm, indicating the possibility to enhance the spawning season.

8d. Productivity

1. Publications in peer review journals

1. Alves Martins, D, Estévez, A, Stickland, N, Simbi, B, Yúfera, M. 2010. Dietary lecithin source affects growth potential and gene expression in Sparus aurata larvae. Lipids 45: 1011-1023.

2. Alves, RN, Cordeiro, O, Silva, TS, Richard, N, de Vareilles, M, Marino, G, Di Marco, P, Rodrigues, PM, Conceição, LEC. 2010. Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics. Aquaculture 299: 57-66.

3. Aragão, C, Costas, B, Vargas-Chacoff, L, Ruiz-Jarabo, I, Dinis, MT, Mancera, JM, Conceicão, LEC. 2010. Changes in plasma amino acid levels in a euryhaline fish exposed to different environmental salinities. Amino Acids 38: 311-317.


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4. Cabrita, E, Sarasquete, C, Martínez-Páramo, S, Robles, V, Beirão, J, Pérez-Cerezales, S, Herráez, MP. 2010. Cryopreservation of fish sperm: applications and perspectives. Journal of Applied Ichthyology 26: 623-635.

5. Calado, R, Pimentel, T, Cleary, D, Dionísio, G, Nunes, C, Lopes da Silva, T, Dinis, MT, Reis, A. 2010. Providing a common diet to different marine decapods does not standardize the fatty acid profiles of their larvae: a warning sign for experimentation using invertebrate larvae produced in captivity. Marine Biology 157: 2427-2434.

6. Conceicão, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2010. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry 36: 1-16.

7. Conceicão, LEC, Yúfera, M, Makridis, P, Morais, S, Dinis, MT. 2010. Live feeds for early stages of fish rearing. Aquaculture Research 36: 1-16.

8. Dias, J, Yúfera, M, Valente, LMP, Rema, P. 2010. Feed transit and apparent protein, phosphorus and energy digestibility of practical feed ingredients by Senegalese sole (Solea senegalensis). Aquaculture 302: 94-99.

9. Diogo, P, Soares, F, Dinis, MT, Cabrita, E. 2010. The influence of ovarian fluid on Solea senegalensis sperm motility. Journal of Applied Ichthyology 26: 690-695.

10. Engrola, S, Dinis, MT, Conceição, LEC. 2010. Senegalese sole larvae growth and protein utilization is depressed when co-fed high levels of inert diet and Artemia since first feeding. Aquaculture Nutrition 16: 457-465.

11. Gómez-Requeni, P, Conceição, LEC, Olderbakk Jordal, A, Rønnestad, I. 2010. A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development. Fish Physiology and Biochemistry 36: 1199-1215.

12. Makridis, P, Libeiro, L, Rocha, R, Dinis MT. 2010. Influence of microalgae supernatant, and bacteria isolated from microalgae cultures, on microbiology, and digestive capacity of larval gilthead seabream, Sparus aurata, and Senegalese Sole, Solea senegalensis. Journal of the World Aquaculture Society 41: 780-790.

13. Martins, CIM, Eding, EH, Verdegem, MCJ, Heinsbroek, LTN, Schneider, O, Blancheton, JP, d'Orbcastel, ER, Verreth, JAJ. 2010. New developments in recirculating aquaculture systems in Europe: A perspective on environmental sustainability. Aquacultural Engineering 43: 83-93.

14. Matos, E, Gonçalves, A, Nunes, ML, Dinis, MT, Dias, J. 2010. Effect of harvesting stress and slaughter conditions on selected flesh quality criteria of gilthead seabream (Sparus aurata). Aquaculture 305: 66-72.

15. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2010. Evaluation of DNA damage as a quality marker for rainbow trout sperm cryopreservation and use of LDL as cryoprotectant. Theriogenology 74: 282-289.


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16. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2010. Fertilization capacity with rainbow trout DNA damaged sperm and embryo developmental success. Reproduction 139: 1-10.

17. Pinto, W, Figueira, L, Ribeiro, L, Yúfera, M, Dinis, MT, Aragão, C. 2010. Dietary taurine supplementation enhances metamorphosis and growth potential of Solea senegalensis larvae. Aquaculture 309: 159-164.

18. Pinto, W, Rodrigues, V, Dinis, MT, Aragão, C. 2010. Can dietary aromatic amino acid supplementation be beneficial during fish metamorphosis? Aquaculture 310: 200-205.

19. Rosa, J, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2010. Serum-related stimulation of the proliferation and mineralization of seabream bone-derived cells. Journal of Applied Ichthyology 26: 245-250.

20. Silva, PIM, Martins, CIM, Engrola, S, Marino, G, Øverli, Ø, Conceicão, LEC. 2010. Individual differences in cortisol levels and behaviour of Senegalese sole (Solea senegalensis) juveniles: evidences for coping styles. Applied Animal Behaviour Science 124: 75-81.

21. Silva, TS, Cordeiro, O, Richard, N, Conceição, LEC, Rodrigues, PM. 2010. Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities. Comparative Biochemistry and Physiology - Part D 6: 82-91.

2. Other publications international (Books, chapters or full papers published in conference proceedings)

1. Cancela, ML, Bargelloni, L, Boudry, P, Boulo, V, Dias, J, Huvet, A, Laizé, V, Lapègue, S, Leite, R, Mira, S, Nielsen, EE, Planas, JV, Roher, N, Sarropoulou, E, Volckaert, FAM. 2010. Genomic Approaches in Aquaculture and Fisheries. In: Cock, JM, Viard, F, Tessamar-Raible, K, Boyen, C (Eds). Introduction to Marine Genomics, Springer-Verlag, Heidelberg, pp. 213-286. ISBN 978-90-481-8616-7.

2. Conceição, LEC, Aragão, C, Rønnestad, I. 2010. Protein metabolism and amino acid requirements in fish larvae. In: Cruz-Suarez, LE, Ricque-Marie, D, Tapia-Salazar, M, Nieto-López, MG, Villareal-Cavazos, DA, Gamboa-Delgado, J (Eds). Avances en Nutrición Acuícola X - Memorias del X Simposio Internacional de Nutrición Acuícola, Universidad Autónoma de Nuevo León, Monterrey, México, pp. 251-264.

3. Anras, L, Boglione, C, Cataudella, S, Dinis, MT, Livi, S, Makridis, P, Marino,G, Ramalho, A, Yúfera, M. 2010. The current status of extensive and semi-intensive aquaculture practices in Southern Europe. Aquaculture Europe 35(2): 12-17.

4. Dias, J, Conceição, LEC, Rodrigues, V, Colen, R, Ramalho Ribeiro, A, Ferreira, H, Aires, T, Silva, JMG, Escórcio, C, Valente, LMP. 2010. Aquaculture growth has triggered environmental concerns. Aquaculture Europe 35(2): 5-11.

5. Quental-Ferreira, H, Ramalho Ribeiro, A, Dias, J, Yúfera, M, Arias, AM, Falcão, M, Serpa, D, Aires, T, Pusão-Ferreira, P, Cunha, ME, Valente, LMP, Dinis, MT; Conceição, L. 2010. Sustainable


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semi-intensive polyculture of seabream and sole in earthern ponds. Aquaculture Europe 35(3): 17-21.

6. Vaz-Pires, P, Ramalho, A, Jesus, A, Soares, F, Conceição, L, Dinis, MT, Ribeiro, L, Pousão-Ferreira, P, Cunha, ME, Quental-Ferreira, H, Yúfera, M, Marino, G, Boglione, C, Cataudella, S, Hussenot, J, Begout, M-L, Makridis, P, Buard, E, Blachier, P. 2010. A joint certification system for sustainable non-intensive aquaculture: a proposal from the SEACASE Project. Aquaculture Europe 35(2): 17-22.

7. Vaz-Pires, P, Escórcio, C, Soares, S, Ramalho, A, Matos, E, Dias, J. 2010. Optimising fish slaughtering procedures for semi-intensive aquaculture. Aquaculture Europe 35(3): 26-28.

8. Ramalho, A, Dinis, MT. 2010. Portuguese aquaculture: current status and perspectives. Aquaculture Europe 35(3): 5-12.

3. Other publications national (Books, chapters or full papers published in conference proceedings)

1. Dinis, MT. 2010. A Aquacultura em Portugal In: Rey-Méndez, M., Fernández Casal, J, Lodeiros, C, Guerra, A (Eds). Foro Rec. Mar. Ac. Rías Gal. 12: 79-85.

4. Master and PhD theses completed

MASTER THESES

Ma, Simão. 2010. Efeitos da adição de antioxidantes nos meios de criopreservação de sémen de peixes Teleósteos. Master thesis in Zootechnical Engineering - Animal Production, Faculty of Veterinary Medicine, Technical University of Lisbon. Supervisor: Maria Teresa Dinis, Co-Supervisor: Fernando Ribeiro Alves Afonso.

Marujo, Débora. 2010. Efeito de um concentrado de microlagas liofilizadas na larvicultura da dourada, Sparus aurata. Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Pedro Pousão-Ferreira.

Peixoto, Lúcia. 2010. Avaliação da inclusão de EVOCOL em dietas formuladas para juvenis de linguado (Solea senegalensis Kaup, 1858). Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Mariano Lastra.

Carrasquinho, Ricardo. 2010. Gestão e maneio de uma unidade de piscicultura em mar aberto na costa sul de Portugal. Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Pedro Pousão-Ferreira.


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PhD THESES

Ribeiro, Ana Rita de Araújo. 2010. Thyroid status in senegalese sole (Solea senegalensis) larvae – effects of iodine and selenium supplementation. PhD thesis in Aquaculture, University of Algarve. Supervisors: Maria Teresa Dinis, Laura Ribeiro (IPIMAR), Mari Moren (NIFES, Norway).

Silva, Joana. 2010. Effectiveness of alternative protein sources in meeting the true amino acid requirements of Senegalese sole. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisors: Luís Conceição, Luísa Valente, Marit Espe.

5. Patents/propotypes

6. Organization of conferences

- National Workshop on “Aquacultura Extensiva e Semi-intensiva: Policultivo, o Caso de Estudo Português”. Workshop in the framework of the SEACASE Project, 19 January 2010, Tavira, Portugal (100 attendees).

- International Workshop on "Sustainable Extensive and Semi-intensive Aquaculture Production in Southern Europe”. Final workshop of the SEACASE Project, 20-21 January 2010, Tavira, Portugal (130 attendees).

- Aquaculture Europe10, organized by the European Aquaculture Society, 5-8 October 2010, Porto, Portugal. MT Dinis - Member of the Steering Committee.

7. Industry contract research

Over the years, the scientific expertise and reliable experimental facilities associated to the Aquaculture Research Group have gained recognition by the aquaculture industry. In 2010, the Aquagroup conducted contract-research activities with two newly created technology-based SME’s:

- Vale I&DT INOVSEA - Production of polychaetes as feed for broodstock fish in aquaculture. Company: INOVSEA Lda.

- Vale I&DT MARSENSING - Potential application of underwater acoustic stimuli in marine aquaculture. Company: MarSensing Lda.

The Aquagroup is also a partner in three projects involving an industrial collaboration:

- GREENDIETS: Formulation and test of new diets with microalgae concentrates for application in the aquaculture sector. Funding by QREN I&DT. Company: NECTON S.A.

- MICALA: Development of a novel microencapsulated feed for fish larvae. Funding by QREN I&DT. Company: SPAROS Lda.


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- PROSPAWN: Implementation of natural spawning for marine fish species in culture - improving the quality of off-spring and animal welfare. Funding by EU-FP7 Research for the Benefit of SMEs. Consortium led by AKVAPLAN-NIVA AS.

Additionally, the Aquagroup as also gained a prize Caixa Agricola do Algarve do ALGARVE 2010 with a project entitled BIOAQUAL.

8. Internationalization

The Aquaculture Research Group has a long standing tradition of associating itself to international cooperation windows. In 2010, 4 PhD students within the Aquagroup benefited from structural collaborations with other European research institutions, namely NIFES (Norway), BIO/UiB (Norway), NTU (former DIFRES, Denmark), and Bodø University College (Norway). This aspect is also reflected by the fact that 13 out of the 21 (62%) articles in international peer-reviewed journals that the Aquagroup published in 2010 were written in collaboration with colleagues from institutions outside Portugal.

The Aquagroup members have been active participants in the following networking structures and Steering Committees at European level:

- COST Action 867 - Welfare of fish in European aquaculture. L Conceição is one of the Portuguese representatives in the Management Committee.

- European Aquaculture Technology and Innovation Platform (EATiP). J Dias is a member of the working groups “Sustainable raw materials for fish feed production” focused on the establishment of the Strategic Research Agenda. L Conceição is the Chair of the workgroup 2 “Hatchery/Nursery production of fry and spat”, of the Thematic Area 3 “Managing the Biological Lifecycle”.

- COST Action FA0801 “LARVANET - Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network”. L Conceição is the Vice-Chair and one of the Portuguese representatives in the Management Committee.

- European Aquaculture Society (EAS) - MT Dinis is one of the board members for 2010-2012.

9. Government/organization contract research


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Aquatic Insular Research



Aquatic Insular Research (AIR)


Ana Isabel de Melo Azevedo Neto


CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto



- EU funding

82.000 EUR, INTERREG III B 2000-2006, Açores-Madeira-Canárias - Gestión sostenible de los recursos marinos, 2009-2012.

55.250 EUR, Programa de Cooperação Transnacional Açores-Madeira-Canárias, PCT-MAC 2007-2013, project GESMAR (MAC/2/C068).

- National funding

306.000 EUR, Direcção Regional para a Ciência e Tecnologia - Unidade piloto de produção de óleo vegetal a partir de microalgas, 2008-2010.

94.000 EUR, Secretaria Regional do Ambiente e do Mar - Estudo da viabilidade de produção de lapa (Patella e Haliotis) em aquacultura intensiva, 2007-2010.


1. Objectives

- Characterize the natural spatial and temporal variability in the structure of benthic communities and determine patterns of distribution and abundance of species;

- Study how the loss of biological diversity can affect community structure;


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- Develop and promote environmental monitoring schemes/long term data acquisition on biodiversity and habitats;

- Develop an index for the classification of coastal waters in the sub-region Macaronesia under the EU Marine Strategy;

- Investigate the taxonomy and biology of selected macroalgae species;

- Investigate new chemical entities from marine organisms with novel pharmacological profiles;

- Investigate the content of lipids, protein and fiber in macroalgae species;

- Investigate the potential of new isolated Azorean strains of Haematococcus in astaxantin production using outdoor photobioreactors;

- Produce outreach programs and materials for the general public and schools;

- Promote research that is relevant to the conservation and sustainable use of marine biodiversity;

- Improve communication and cooperation between researchers and policy makers to facilitate the design and implementation of knowledge based policies and action plans, at regional, National and European level.


- Illegal harvesting of limpets in the Azores was confirmed to be a common practice both inside and outside protected areas. Lack of enforcement of regulations is a likely reason for the failure of legislation to protect limpet populations and facilitate stock recovery.

- The dominance of the Azorean intertidal by algal turfs was shown to be a result of the chronic exploitation of limpets. Therefore, conservation strategies aimed at increasing limpet abundances should find ways to restore intertidal communities to their pre-disturbed state.

- Developement of a tool for the assessment of ecological quality of rocky shores in the Macaronesian archipelagos.

- Several additions to the Azorean seaweed flora.

- Typification and status of the red calcareous alga Amphiroa cryptarthrodia Zanardini.

- Coralline algae were confirmed to be an ecological important group on the Azorean intertidal.

- C. elongata was found to be a useful bioindicator of heavy metal contamination.

- Several species of macroalgae were confirmed to be good sources of fiber, protein and lipids.


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- The culture system developed for astaxantin production from the microalgae Haematococcus was shown to have potential for upscaling.

- Astaxantin production from Azorean Haematococcus strains was shown to be higher than most commercial strains.

8d. Productivity


1. Athanasiadis, A, Neto, AI. 2010. On the occurrence of Mesophyllum expansum (Philippi) Cabioch et Mendoza (Melobesioideae, Corallinales, Rhodophyta) in the Mediterranean, the Canary Isles and the Azores. Botanica Marina 53: 333-341.

2. Canning-Clode, J, Maloney, KO, Mcmahon, SM, Wahl, M. 2010. Expanded view of the local-regional richness relationship by incorporating functional richness and time: a large-scale perspective. Global Ecology and Biogeography 19: 875-885.

3. Couto, RP, Neto, AI, Rodrigues, AS. 2010. Metal concentration and structural changes in Corallina elongata (Corallinales, Rhodophyta) from hydrothermal vents. Marine Pollution Bulletin 60: 509-514.

4. Delgado, C, Canário, AVM, Dellinger, T. 2010. Sex ratios of loggerhead sea turtles Caretta caretta during the juvenile pelagic stage. Marine Biology 157: 979-990.

5. Gabriel, D, Parente, MI, Neto, AI, Raposo, M, Schils, T, Fredericq, S. 2010. Phylogenetic appraisal of the genus Platoma (Nemastomatales, Rhodophyta), including life history and morphological observations on P. cyclocolpum from the Azores. Phycologia 49 (1): 2-21.

6. Martins, GM, Thompson, RC, Neto, AI, Hawkins, SJ, Jenkins, SR. 2010. Enhancing stocks of the exploited limpet Patella candei via modifications in coastal engineering. Biological Conservation 143: 203-211.

7. Martins, GM, Thompson, RC, Neto, AI, Hawkins, SJ, Jenkins, SR. 2010. Exploitation of intertidal grazers as a driver of community divergence. Journal of Applied Ecology 47: 1282-1289.

8. McCarthya, AL, Heppella, S, Royerb, F, Freitas, C, Dellinger, D. 2010. Identification of likely foraging habitat of pelagic loggerhead sea turtles (Caretta caretta) in the North Atlantic through analysis of telemetry track sinuosity. Progress in Oceanography 86: 224-231.

9. Parente, MI, Fletcher, RL, Neto, AI, Tittley, I, Sousa, AF, Draisma, S, Gabriel, D. 2010. Life history and morphological studies of Punctaria tenuissima (Chordariaceae, Phaeophyceae), a new record for the Azores. Botanica Marina 53 (3): 223-231.

10. Rosas-Alquicira, EF, Riosmena-Rodríguez, R, Neto, AI. 2010. The typification and status of Amphiroa cryptarthrodia (Corallinales, Rhodophyta). Phycological Research 58: 248-257.


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11. Silva, A, Brotas, V, Orive, E, Neto, AI. 2010. First records of Ostreopsis heptagona, Ostreopsis cf. siamensis and Ostreopsis cf. ovata in Atlantic waters of the Azores archipelago (Portugal). Harmful Algal News 42: 1-2.

In press

12. León-Cisneros, K, Riosmena-Rodríguez, R, Neto AI. (in press). A re-evaluation of Scinaia (Nemaliales, Rhodophyta) in the Azores. Helgoland Marine Research, doi: 10.1007/s10152-010-0207-2.

13. León-Cisneros, K, Nogueira, E, Riosmena-Rodríguez, R, Neto, AI. (in press). Life-cycle of Scinaia interrupta (Nemaliales; Rhodophyta). Journal of Applied Phycology, doi: 10.1007/s10811-010-9605-8.

14. Martins, GM, Jenkins, SR, Hawkins, SJ, Neto, AI, Medeiros, AR, Thompson, RC. (in press). Illegal harvesting affects the success of fishing closure areas. Journal of the Marine Biological Association UK, doi: 10.1017/S0025315410001189.

15. Patarra, RF, Paiva, L, Neto, AI, Lima, E, Baptista, J. (in press). Nutritional value of selected macroalgae. Journal of Applied Phycology, doi: 10.1007/s10811-010-9556-0.

16. Rosas-Alquicira, EF, Riosmena-Rodríguez, R, Neto, AI. (in press). Segregation characters used within Amphiroa (Corallinales, Rhodophyta) and taxonomic reevaluation of the genus in the Azores. Journal of Applied Phycology, doi: 10.1007/s10811-010-9606-7.

17. Rosas-Alquicira, EF, Riosmena-Rodríguez, R, Afonso-Carrillo, J, Neto, AI. (in press). Taxonomic biodiversity of geniculate coralline red algae (Corallinales; Rhodophyta) from the Macaronesia region: summary and analysis. Helgoland Marine Research, doi: 10.1007/s10152-010-0209-0.


1. Jenkins, SR, Martins, GM. 2010. Chapter 4 - Succession on Hard Substrata. In: Dürr S, Thomason JC (Eds). Biofouling. Wiley-Blackwell, pp. 60-72.


1. Wallenstein, FM, Peres, SD Xavier, ED, Neto, AI. 2010. Phytobenthic communities of intertidal rock pools in the eastern islands of Azores and their relation to position on shore and pool morphology. Arquipélago. Life and Marine Science 27: 9-20.


MASTER THESES

Pereira, Nuno Miguel Campos Mendes Sousa. 2010. Avaliação do potencial aquícola da população de amêijoas Venerupis aurea (Gmelin, 1791) da ilha de S. Jorge (Açores). Master


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thesis in Sea Sciences – Marine Resources, ICBAS, University of Porto. Supervisor: Ana I Neto, Co-supervisor: Domitília Matias (IPIMAR).

Álvaro, Nuno Miguel Vaz. 2010. Proposta de plano de monitorização para a área protegida para a gestão de habitats ou espécies do Ilhéu de Vila Franca do Campo. Master thesis in Coastal Management, University of the Azores. Supervisor: Fátima Alves (University of Aveiro), Co-supervisor: Ana I Neto.

PhD ThESES

Rosas Alquicira, Edgar Francisco. 2010. Filogeografia de algas Corallinales (Rhodophyta) das Californias. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto, Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).

León Cisneros, Karla. 2010. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto, Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).



- International Symposium FloraMac2010, 23-25 September 2010, Ponta Delgada, Azores. Ana I Neto – Member of the Organizing and Scientific Committee.


The group develops research in collaboration with the company ALGICEL for industrial microalgae production.


The main activities at the international level were:

- The active collaboration in the preparation of the research proposal LAUMACAT - Diversity and phylogenetic relationships on the benthic marine algae with pharmacological potencial: the Laurencia complex (Rhodophyta) in Macaronesia archipelagos, tropical and subtropical Atlantic. Phase III. Subdirección General de Proyectos de Investigación, Gobierno de España. The project, involving 11 partners (University of Laguna, Tenerife, The Canaries; CIIMAR/CIMAR; University of Azores; Environment Agency of Brasil - Botanical Institute; University of Iztapalapa, Mexico; State University of Rio de Janeiro, Brasil; University of São Paulo, Brasil; University of Madeira; Marine Biology Station of Funchal; University of Messina, Italy; University of Espírito Santo, Brasil), was approved and will start on January 2011.


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- The active collaboration in the preparation of the proposal OCEANEEDS - Optimized Culture of Marine Organisms for Human Health and Environmental Applications. 7th Framework Program (delivered in January 2010). The project, involving 9 partners (PHARMAMAR, Spain; Arvam, France; Mata Karang, Indonesia; Marinomed, Viena, Austria; University of Almeria, Spain; University of Bonn, German, University of Wageningen, Netherlands; CIIMAR/CIMAR; Geological Institute Igme-Csic, Madrid, Spain), was not approved.

- The active collaboration in the preparation of the proposal Evaluación de la taxonomía del género Amphiroa (Corallinales, Rhodophyta) en la costa de Oaxaca. In collaboration with Universidad del Mar, Oaxaca, Mexico. Under evaluation.

- Joint coordination of graduated students at MSc and PhD levels with several internacional institutions, e.g. the Universities of Bangor, Plymouth and Heriot-Watt (UK) and Baja California del Sur (Mexico).

- Coordination of small research projects under the European Program Eurodisseia.


1. Neto, AI, Prestes, ACL, Álvaro, NMV, Xavier, ERN. 2010. Estudo da viabilidade de produção de Lapa (Patella e Haliotis) em aquacultura intensiva. Departamento de Biologia, Universidade dos Açores, 21p.

2. Neto, AI, Álvaro, NV, Azevedo, JMN. 2010. Estudo de impacte ambiental. Porto das Pipas – Angra do Heroísmo: terminal de cruzeiros. Caracterização da flora e fauna marinhas, avaliação do impacte ambiental, medidas minimizadoras e monitorização. Departamento de Biologia, Universidade dos Açores, 13p.


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Biogeographical Ecology and Evolution (BEE)



Biogeographical Ecology and Evolution


Maria Ester Tavares Alvares Serrao





- FCT pluriannual funding.

- FCT National funding

638 EUR, POCI/MAR/57342/2004, NETWORK.

44.912 EUR, PTDC/MAR/65461/2006, MEGIKELP.

58.163 EUR, PTDC/MAR/72630/2006, SOPA.

20.218 EUR, PTDC/BIA-BDE/68730/2006, METAPOPULAÇÕES.

86.082 EUR, PTDC/MAR/64749/2006, IBISA.

36.890 EUR, PTDC/MAR/70921/2006, CAULERPA GENETICS.

2.267 EUR, EURODEEP/0002/2007, DEECON.

18.194 EUR, PTDC/BIA-BDE/74349/2006, PILARES DE HÉRCULES.

18.905 EUR, MARIN-ERA/MAR/0001/2008, MARINERA SHIFTING.

53.411 EUR, PTDC/MAR/104477/2008, POLYANDRY.

43.579 EUR, PTDC/BIA-BEC/103916/2008, PERMYT.

82.152 EUR, PTDC/MAR/099887/2008, RIASCAPEGEN.


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64.259 EUR, PTDC/MAR/108013/2008, CANARYGRASS.

33.307 EUR, PTDC/MAR/099698/2008, NORIGENOMICS.

86.268 EUR, PTDC/MAR/108105/2008, HYBRID.

85.521 EUR, PTDC/AAC-CLI/109108/2008, EDGES.

- European funding

81.972 EUR, European Commission, Contract Nr. 226248, ENV.2008.1.1.5.2, ATP.


1. Objectives

We aim at understanding marine evolutionary ecology and biogeography.

A large focus of our current research in evolutionary ecology is the nature of barriers limiting gene flow in marine populations, a first step towards speciation. Another main goal is the understanding of mating systems and their role in driving reproductive isolation, speciation, hybrid zones. Selective gradients of environmental stress in parapatric species are a main study model to test drivers of ecological speciation in sister species and the role of local selection in secondary contact zones following invasions.

In biogeographical theory we empirically test predictions from evolutionary ecology of species´ranges, whereby distributional edges and marginal habitats are expected to have lower diversity and higher differentiation which trade-off with higher selective pressures for stress-driven local adaptation at range edges, reducing their evolvability.

We track genetic imprints of ocean-climate cycles from Pleistocene ice ages, on several taxa of pelagic and benthic marine species across various oceans and seas, inferred from genetic structure and molecular phylogenies. We look for imprints of historical climate change on retreating and leading edge populations of marine species.

Biological models are varied marine organisms such as plants, algae, animals, their associated micro-organisms, using a variety of approaches, from molecular to experimental ecology and phylogenetic reconstruction.

We focus particular research objectives on ecosystems that play important roles in our planet, for global climate and ocean productivity (polar phytoplankton metatranscriptomics), for living under extreme conditions for life (dispersal along deep sea vents), or for sustaining marine biodiversity (dispersal and diversity of kelp forests and seagrass meadows), for their role as invasive (evolutionary ecology).

All these objectives require novel developments in genomics markers and software.


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Major discoveries:

I. Speciation:

- A novel Fucus speciation event is driven by natural selection and high selfing;

- Speciation history, timings and drivers were inferred for Holothuria and pearl oysters.

II. Phylogeography, gene flow:

- Iberia contains high and private genetic diversity for cold-temperate species, and was as source of post-glacial recolonization, during which introgressed organelles in Fucus expanded northwards by surfing;

- Along Mid-Atlantic Ridge thermal vents, shrimp populations suffered a bottleneck or founder event followed by a recent demographic expansion;

- Gene flow across giant kelp beds in California is best explained using seascape features;

- White seabream show high connectivity across Iberian and Mediterranean sites, but are genetically distinct in the Azores;

- Genetic structure of Holothuria polii at a coastal lagoon shows recent demographic growth and environmental correlates;

- Past vicariance and current low connectivity were inferred in a Mediterranean seagrass;

- Seagrass meadows in Brittany are a mosaic of clones with distinct origins in space and time;

- A single surviving clone of the seagrass Zostera noltii in the Canary Islands, has its closest relatives in northern Morocco, the best donor for future restoration.

- Global analyses of the genetic diversity-stability relationship in seagrasses contradicts predictions in revealing a negative relationship and a threshold below which no relationship exists.

III. Stress-driven evolution/ecology:

- Rapid (thousands yrs) stress-response divergence occurred between sympatric F. radicans and F. vesiculosus in the Baltic Sea.

- During heat shock in Fucus serratus, there is overexpression of photoprotective and heat-shock genes.

- Competitive outcome of invasive and indigenous intertidal mussels in South Africa is determined by local habitat variability and is influenced by gaping differences mediating temperature and desiccation stress.


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IV. Technical development:

- Microsatellite markers for a seahorse.

8d. Productivity


1. Alberto, F, Raimondi, PT, Reed, DC, Coelho, NC, Leblois, R, Whitmer, A, Serrão, EA. 2010. Habitat continuity and geographic distance predict population genetic differentiation in giant kelp. Ecology 91: 49-56.

2. Arnaud-Haond, S, Marbà, N, Diaz-Almela, E, Serrão, EA, Duarte, CM. 2010. Comparative analysis of stability - genetic diversity in seagrass (Posidonia oceanica) meadows yields unexpected results. Estuaries and Coasts 33: 878-889.

3. Barluenga, M, Austerlitz, F, Elzinga, JA, Teixeira, S, Goudet, J, Bernasconi, G. 2010. Fine scale spatial genetic structure and gene dispersal in Silene latifolia. Heredity: 1-12.

4. Becheler, R, Diekmann, O, Hily, C, Moalic, Y, Arnaud-Haond, S. 2010. The concept of population in clonal organisms: mosaics of temporally colonized patches are forming highly diverse meadows of Zostera marina in Brittany. Molecular Ecology 19: 2394-2407.

5. Billard, E, Serrão, EA, Pearson, GA, Destombe, C, Valero, M. 2010. Fucus vesiculosus and spiralis species complex: a nested model of local adaptation at the shore level. Marine Ecology Progress Series 405: 163-174.

6. Borrero-Pérez, GH, Gómez-Zurita, J, González-Wangüemert, M, Marcos, C, Pérez-Ruzafa, A. 2010. Molecular systematics of the genus Holothuria in the Mediterranean and Northeastern Atlantic and a molecular clock for the diversification of the Holothuriidae (Echinodermata: Holothuroidea). Molecular Phylogenetics and Evolution 57: 899-906.

7. Diekmann, OE, Gouveia, L, Perez, JA, Gil-Rodriguez, C, Serrao, EA. 2010. The possible origin of Zostera noltii in the Canary Islands and guidelines for restoration. Marine Biology 157: 2109-2115.

8. González-Wangüemert, M, Cánovas, F, Pérez-Ruzafa, A, Marcos, C, Alexandrino, P. 2010. Connectivity patterns inferred from the genetic structure of white seabream (Diplodus sargus L.). Journal of Experimental Marine Biology and Ecology 383: 23-31.

9. Lago-Leston, A, Mota, C, Kautsky, L, Pearson, GA. 2010. Functional divergence in heat shock response following rapid speciation of Fucus spp. in the Baltic Sea. Marine Biology 157: 683-688.

10. Neiva, J, Pearson, GA, Valero, M, Serrao, EA. 2010. Surfing the wave on a borrowed board: range expansion and spread of introgressed organellar genomes in the seaweed Fucus ceranoides L. Molecular Ecology 19: 4812-4822.


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11. Nicastro, KR, Zardi, GI, McQuaid, CD. 2010. Differential reproductive investment, attachment strength and mortality of invasive and indigenous mussels across heterogeneous environments. Biological Invasions 12: 2165-2177.

12. Nicastro, KR, Zardi, GI, McQuaid, CD, Stephens, L, Radloff, S, Blatch, GL. 2010. The role of gaping behaviour in habitat partitioning between coexisting intertidal mussels. BMC Ecology 10: 17.

13. Pearson, GA, Hoarau, G, Lago-Leston, A, Coyer, JA, Kube, M, Henckel, K, Serrão, EA, Corre, E, Stam, WT, Olsen, JL. 2010. An expressed sequence Tag (EST) analysis of the intertidal brown seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in response to abiotic stressors. Marine Biotechnology 12: 195-213.

14. Serra, IA, Innocenti, AM, Di Maida, G, Calvo, S, Migliaccio, M, Zambianchi, E, Pizzigalli, C, Arnaud-Haond, S, Duarte, CM, Serrão, EA, Procaccini, G. 2010. Genetic structure in the Mediterranean seagrass Posidonia oceanica. Disentangling past vicariance events from contemporary patterns of gene flow. Molecular Ecology 19: 557-568.

15. Teixeira, S, Cambon-Bonavita, M, Serrao, EA, Desbruyeres, D, Arnaud-Haond, S. 2010. Recent population expansion and connectivity in the hydrothermal shrimp Rimicaris exoculata along the Mid Atlantic Ridge. Journal of Biogeography 38: 564-574.

16. Vergara-Chen,C, González-Wangüemert, M, Marcos, C, Pérez-Ruzafa, A. 2010. Genetic diversity and connectivity remain high in Holothuria polii (Delle Chiaje 1823) across a coastal lagoon-open sea environmental gradient. Genetica 138: 895-906.

In press

17. Van de Vliet, MS, Diekmann, OE, Serrão, ETA. (in press). Highly polymorphic microsatellite markers for the Short-snouted seahorse (Hippocampus hippocampus), including markers from a closely related species the Long-snouted seahorse (Hippocampus guttulatus). Conservation Genetics Resources, doi: 10.1007/s12686-009-9022-y.




MASTER THESES

Lima dos Reis, Jandir João. 2010. The genetic mating system in the black striped pipefish, Syngnathus abaster (Risso, 1827) in Ria Formosa, revealed by microsatellites. Master thesis in


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Marine Biology, University of Algarve. Co-supervisors: Onno Diekmann, Ester Serrao, Filipe Alberto.

Pires, Rita. 2010. Padrões temporais e verticais de pequena escala na ocorrência de larvas de peixe na Ria de Aveiro, Portugal. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).

Afonso, João Marcos da Silva. 2010. Padrões verticais e inter-anuais nas assembleias de larvas de peixe junto ao substrato rochoso do Parque Marinho da Arrábida. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).

Berkovic, Buga. 2010. Effect of habitat fragmentation on bivalve larvae recruitment: study of Pinna nobilis and Pectinidae species larvae. Master thesis in Marine Biodiversity and Conservation, University of Algarve. Co-supervisors: Ester Serrao, Iris Hendriks (IMEDEA, CSIC).

Purroy Albet, Ariadna. 2010. Spatial assessment and impact of artisanal fisheries´activity in Cap de Creus. Master thesis in Marine Biodiversity and Conservation, University of Algarve. Co-supervisors: Ester Serrao, Rafael Sardá and Susana Requena (CSIC, Barcelona).

Barradas, Ana Rita. 2010. Laminaria ochroleuca canopy effect on intertidal under storey benthic assemblages in northern Portugal. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Ester Serrao, Filipe Alberto.

Rocha, Patrícia Carolina Rendall. 2010. Descrição do desenvolvimento larvar de Tripterygion delaisi (Pisces: Tripterygiidae). Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).




Awards/donations from industry / private foundations:

- Fundação EDP - Biodiversidade - award for project FINDKELP: 35.000 EUR.

- BES, Banco Espírito Santo - Honorary mention of Prize BES - Biodiversidade for project FINDKELP: 3.000 EUR.

- Throttleman – Inaqua, Oceanário de Lisboa, National Geographic Channel award for project Deep Reefs: 25.000 EUR.


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Nearly all of the team´s research projects and publications were conducted in collaboration with various international teams of experts in each field (examples below).

All our PhD students have international co-supervisors, selected with the main goal of broadening their experience and internationalization of their careers.

Two team members gave invited plenary talks at international meetings in 2010.

Our team includes one of the local coordinators of the European MSc degree in Marine Biodiversity and Conservation, referees for 33 scientific journals, one associate editor of European Journal of Phycology, one referee for European Research Council grants.

Examples of international collaborative publications:

- Aires, T, Marbà, N, Cunha, RL, Kendrick, GA, Walker, DI, Serrao, EA, Duarte, CM, Arnaud-Haond, S. 2011. Marine Ecology Progress Series 421: 117-130.

- Alberto, F, Raimondi, PT, Reed, DC, Coelho, NC, Leblois, R, Whitmer, A, Serrão, EA. 2010. Ecology 91: 49-56.

- Becheler, R, Diekmann, O, Hily, C, Moalic, Y, Arnaud-Haond, S. 2010. Molecular Ecology 19: 2394-2407.

- Billard, E, Serrão, EA, Pearson, GA, Destombe, C, Valero, M. 2010. Marine Ecology Progress Series 405: 163-174.

- Borrero-Pérez, GH, Gómez-Zurita, J, González-Wangüemert, MC, Pérez-Ruzafa, A. 2010. Molecular Phylogenetics and Evolution 57: 899-906.

- Coyer JA, Hoarau G, Costa J, Hogerdijk B, Serrão EA, Billard E, Valero M, Pearson GA, Olsen, J. 2011. Molecular Phylogenetics and Evolution 58: 283-296.

- Moalic, Y, Arnaud-Haond, S, Perrin, C, Pearson, GA, Serrao, EA. 2011. BMC Evolutionary Biology 11: 33.

- Neiva, J, Pearson, GA, Valero, M, Serrao, EA. 2010. Molecular Ecology 19: 4812-4822.

- Nicastro, KR, Zardi, GI, McQuaid, CD, Stephens, L, Radloff, S, Blatch, GL. 2010. BMC Ecology 10: 17.

- Pearson, GA, Hoarau, G, Lago-Leston, A, Coyer, JA, Kube, M, Henckel, K, Serrão, EA, Corre, E, Stam, WT, Olsen, JL. 2010. Marine Biotechnology 12: 195-213.

- Serra, IA, Innocenti, AM, Di Maida, G, Calvo, S, Migliaccio, M, Zambianchi, E, Pizzigalli, C, Arnaud-Haond, S, Duarte, CM, Serrão, EA, Procaccini G. 2010. Molecular Ecology 19: 557-568.


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- Teixeira, S, Cambon-Bonavita, M, Serrao, EA, Desbruyeres, D, Arnaud-Haond, S. 2011. Journal of Biogeography 38: 564-574.



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Biophysics



Biophysics


Maria Leonor Nunes Ribeiro Cruzeiro





- FCT Pluriannual funding

- National funding

1.111 EUR. FCT, PTDC/SAU-GMG/098274/2008 - Intermediates states: At the crossroads of pathways to folding and pathways to disease.

20000 CPU hours. L Cruzeiro is PI in the supercomputing project “Um mecanismo cinético para a formação da estrutura das proteínas” in the supercomputer “Milipeia” of the Laboratory for Advanced Computing of the University of Coimbra.

ca 23.340 EUR per annum, Postdoctoral grant for Dr. Holly Freedman, funded by FCT, ref. SFRH/BPD/41143/2007. Supervisor: L Cruzeiro.


1. Objectives

The ultimate aim of the Biophysics Group is to understand how proteins fold and also how they function, something that is not only of interest for Marine Biology but also for Biology in general, as well as for Biomedicine and for the Biotechnology and the Pharmaceutical industries.

Our scientific approach is based on two fundamental assumptions: a) the free energy landscape of proteins includes many kinetic traps, that is, many folded conformations that have the same free energy as the native state, and b) protein folding and function involve the storing and propagation of energy in the form of vibrational excited states (VES), something that LC has


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named the VES hypothesis. The model we use for energy transfer in proteins is the Davyodv/Scott model in which the vibrational state usually considered is the amide I vibration; however, but the NH stretch vibration, which is two times more energetic, may also be important since its energy corresponds to the total energy released in the hydrolysis of ATP.

In 2010 three specific objectives were pursued: 1) further simulations were performed to show that the free energy landscape of proteins is multi-funnel shaped and to develop further the kinetic mechanism for protein folding proposed before; 2) the modified code of GROMACS in which the influence of quantum vibrational excited states on protein dynamics is included was applied to protein structural rearrangements associated with mechanical work, and 3) research on the the solectron concept for electron (super)conductivity in condensed matter proposed by Prof. Manuel G. Velarde, of the Instituto Pluridisciplinar of the Universidad Complutense, Madrid, Spain, was extended to electron transfer in proteins.


Concerning objective 1) simulations of four proteins, representative of the four main CATH protein classes, with a duration of at least 50 nanoseconds (50 times longer than our previous simulations) confirmed the thermal stability of four non-native states, one for each of the proteins, suggesting that the free energy landscape of those four proteins is not funnel shaped as is currently assumed. These results were obtained in collaboration with Prof. Paulo A. Lopes, of CITI/UNL and have also led to the renewal of a provisional patent application with the aim of finding a USA partner since according, to European Patent law, a computer algorithm is not patentable.

Concerning objective 2) we found that the presence of an amide I vibration deca-alanine can lead to a more helical conformation of this peptide, in just a few picoseconds, provided the change of the quantum vibrational energy with the hydrogen bond compression is greater than a few hundred picoNewtons, a value that is possible for the quantum NH stretch vibrations of peptides. These results have been published in Physical Review B. So far, the quantum modes in the modified GROMACS code are treated as stationary states while in fact they are transient states. CP2K is a quantum software that can describe transient states in mixed quantum-classical systems. Dr. Holly Freedman, a postdoctoral collaborator in the Biophysics group, visited Prof. Marta Ramos in November 2010 and in order to learn how to install and use CP2K, and also visited the IBM group in Zurich who is developing that software.

Concerning objective 3), a manuscript entitled “On the possibility of electric transport mediated by long living intrinsic localized solectron modes” was finalized for publication and a program to simulate an electron pair with the Solectron-Hubbard Hamiltonian was written. Dr. Oliva Garcia Cantu Ros and Prof. Manuel G. Velarde , of the Instituto Pluridisciplinar of the Universidad Complutense, Madrid, Spain, visited CCMAR in June and September, respectively, and while LC visited Madrid in February, August and November, also to work on electron transfer from donors to acceptors in proteins.


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8d. Productivity


1. Freedman, H, Martel, P, Cruzeiro, L. 2010. Mixed quantum-classical dynamics of an amide I vibrational excitation in a protein α-helix. Physical Review B 82: 174308.


1. Cruzeiro, L. 2010. Protein Folding. In: Springborg, M. (Ed). Specialist Periodic Reports of the Royal Society of Chemistry, UK, Chem. Modell., vol. 7, pp. 89-114. ISBN: 978-1-84755-075-0.

2. Cruzeiro, L, Lopes, PA. 2010. Parallel Computing and Protein Design. In: Proceedings of the 4th Iberian Grid Infrastructure Conference Proceedings, 24-27 May, Braga, Portugal. Proença, A, Pina, A, Tobío, JG, Ribeiro, L (Eds), pp. 132-143. ISBN 978-84-9745-549-7.




Cruzeiro, L. 2010. Método para deduzir a estrutura tridimensional de uma proteína a partir da sequência de aminoácidos. Portuguese Provisional Patent Application nº 105299, 20th September 2010.


L Cruzeiro organized the Mini workshop on “Computer Simulations of Nanobiological Systems”, CCMAR, University of Algarve, 27 May 2010.



The collaboration of the Biophysics group with Prof. Manuel Garcia Velarde of the Instituto Pluridisciplinar of the Universidad Complutense de Madrid is now funded by the EXPLORA grant (see section 1.4). In 2010, the visits to CCMAR by Dr. Oliva Garcia Cantu Ros, a postdoctoral researcher in Prof. Velarde’s group, between 20-28 June and by Prof. Velarde himself in September were funded by the EXPLORA project, as well as the three visits by L Cruzeiro to Madrid in February, August and November. These visits resulted in the writing of manuscripts for publication (two of which will appear in 2011).


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In spite of the fact that a collaborative grant submitted by L Cruzeiro and by Prof. Michael Springborg of the University of Saarland, Saarbrücken, Germany, to Acções Integradas Luso-Alemães was turned down, the collaboration with Prof. Springborg continues, and a student of Prof. Springborg has started to do the quantum calculations to evaluate the coupling parameters needed for the simulation of vibrational energy storage and transfer in proteins.

The Biophysics group has also an on-going collaboration with the Nonlinear Physics Group of the University of Seville, Seville, Spain and in particular with Prof. Francisco Romero Romero (the leader) and Dr. Jesús Cuevas Maraver, on the general theme of protein folding and function. In particular we have been studying quantum thermalization schemes based on Lindblad operators.

Finally, L Cruzeiro is also an honorary research fellow at the Mathematics Department of Heriot-Watt University, Edinburgh, Scotland, UK, where the Biophysics group collaborates with Prof. Chris Eilbeck on the interaction of quantum particles with mechanical vibrations, with models that are an extension of those applied to energy transfer in proteins.



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Cellular and Inorganic Biochemistry (CIB)



BioVanadium, now consolidated as Cellular and Inorganic Biochemistry (CIB)


Manuel Aureliano Pereira Martins Alves

3. Location of group (Host institution)




Ca. 4.000 EUR


1. Objectives

Within the research that the group of Cellular and Inorganic Biochemistry developed at CCmar (in collaboration with others research groups), the main objectives for 2010 were the following; i) Study the interaction of decavanadate with a muscle protein, actin; ii) Study the effects of peroxynitrite on the structure and function of a muscle protein, myosin; iii) Study the effects of oxidative on neurons and iv) Study the effects of immunosuppressive agents in adipocytes.


Within the research that the group of Cellular Inorganic Biochemistry developed at CCmar (in collaboration with others research groups), the main objectives for 2010 were the following; i) Study the interaction of decavanadate with a muscle protein, actin; ii) Study the effects of peroxynitrite on the structure and function of a muscle protein, myosin; iii) Study the effects of oxidative on neurons and iv) Study the effects of immunosuppressive agents in adipocytes.


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8d. Productivity


1. Ramos, S, Moura, JJG, Aureliano, M. 2010. Actin as a potential target for decavanadate, Journal of Inorganic Biochemistry 104: 1234-1239.

2. Tiago, T, Palma, PS, Gutierrez-Merino, C, Aureliano, M. 2010. Peroxynitrite-mediated oxidative modifications of myosin and implications on structure and function. Free Radical Research 44: 1317-1327.

3. Marques-da-Silva, D, Samhan-Arias, AK, Tiago, T, Gutierrez-Merino, C. 2010. L-type calcium channels and cytochrome b(5) reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane. Journal of Proteomics 73: 1502-1510.


1. Ramos, S, Moura, JJG, Aureliano, M. Decavanadate effects in actin structure and function. FEBS Journal 277: 190.

2. Pereira, MJ, Palming, J, Svensson, MK, Aureliano, M Eriksson, J. 2010. Immunosuppresive agents alter insulin signalling and glucose and lipid metabolism in human subcutaneous adipocytes. Diabetologia 53: 710.



MASTER THESES

Pereira, Ana Margarida. 2010. Efeito de complexos de vanádio na bomba de cálcio de retículo sarcoplasmático. Master thesis in Biotechnology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Manuel Aureliano.



- 4th SPB Clinical Biochemistry Workshop, University of Algarve, 29 January 2010, Faro, Portugal. Manuel Aureliano – Member of the Organizing Committee.


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Cellular, Molecular and Analytical Studies (LECEMA)



Cellular, Molecular and Analytical Studies (LECEMA)


Eduardo Jorge Sousa Rocha





- 35.000 EUR, FCT Pluriannual funding.

- National funding

66.781 EUR, FCT, PTDC/MAR/68885/2006, Lipid metabolism disruption in marine fish by xenobiotic ligands of peroxisome proliferator-activated receptors (PPARs). 14.421 EUR, FCT, PTDC/MAR/70436/2006, An integrative study on the toxicopathic lesions in Portuguese estuarine fishes - Assessing injury impact and toxicogenomic implications in experimental models. 17.228 EUR, FCT, PTDC/MAR/68106/2006, The modulation of retinoic acid signalling pathways by environmental pollutants in teleosts. 11.082 EUR, FCT, PTDC/MAR/68106/2006, The modulation of retinoic acid signalling pathways by environmental pollutants in teleosts. 3.682 EUR, FCT, PTDC/MAR/105199/2008, O repertório de receptores nucleares em invertebrados: investigações evolutivas e de disrupção endócrina. 500 EUR, FCT, PTDC/CVT/102453/2008, Ovary apoptosis in zebrafish (Danio rerio): pathways characterization, role in sexual differentiation and as endocrine disruptors chemicals target.


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1. Objectives

The general strategic scientific objectives for the group set up back in 2003 were to build a multidisciplinary group and to establish a well-equipped lab space at the CIIMAR new facilities in Porto. We aimed to build a team able to support multidisciplinary studies in field and laboratory studies, and well positioned to establish cooperation with other national and international groups. The research efforts at the LECEMA (formerly LECEM) soon targeted both fundamental and applied studies, correlating structure with function in selected fish and molluscs, either in normal and pathological scenarios (namely in toxicological contexts). Some emphasis was as is being put on influences of steroid hormones and xenobiotics in unexplored targets, from molecular (eg, mechanisms of imposex and of PPARs involvement in peroxisomal changes) to organ levels (eg, liver responses to subacute toxicity). More recently we aimed to expand our expertise to chemical analysis in parallel to biochemical approaches, aiming data integration. As a dynamic group, we established a strategy to expand our studies to in vitro approaches, namely for getting mechanistic insights on some phenomena we discovered in vivo. We will increasingly use in vitro systems to study fundamental and toxicological problems.

As to non-experimental studies in aquatic biology, our main objective has been to contribute for a better knowledge of the structure and function of the fish liver, and other related aspects of the digestive tract, considering gender and seasonal effects. More recently, those studies also involved the kidney, establishing a comparative approach between events detected in liver and that are for example explored as a new thesis of endocrine (estrogenic) modulation of peroxisomal function. Comparative studies with phylogenetic emphasis were also made in the digestive apparatus of molluscs, including hydrothermal-vent bivalves from the Azores. These approaches aimed to provide sound baseline data to support field and experimental (inc. toxicopathological) studies, which were initiated combining biomarkers with analytical chemistry targeting toxicants.

As to experimental studies, our aim has been to target biological problems related with endocrine regulation vs disruption in aquatic animals, namely on liver and reproductive system. Examples of those are: 1) how and to what extent fish peroxisomes are regulated and disrupted by estrogenic compounds and xenobiotic ligands of PPARs; and 2) what are the fine molecular mechanisms involved in the development of male penis and female imposex whereas expanding this approach for studying the impact of known endocrine disrupting chemicals (agonists and antagonists) to invertebrate nuclear receptors. In additional experiments, in cooperation with other research groups at CIMAR and from other institutions, we want to become a reference in implementing histopathology for diagnosis of toxicophatic lesions and also testing new quantitative approaches at tissue level for evaluating the intensity of effects of reference xenobiotics, like pesticides.

We also set the aim to put our expertise in some more ambitious field studies in toxicology, in cooperation with other groups, with a focus on endocrine disruption biological targets and their correlation with a wide range of contaminants for which we validated methods. We aim to cover a wider range of relevant contaminants, particularly those with known endocrine / neuroendocrine disruption effects, but also other such as pharmaceuticals. Under a current project, we are continuing to conduct surveys in several main estuaries in Portugal. In the near


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future, with partners already established, we aim to offer integrated databases and innovative approaches such as the one inserted in a very recently funded project for tackling endocrine disruption by studying an invertebrate repertoire of nuclear receptors.

Besides research, we want to continue to give support to post-graduate studies. Such aim is currently being fulfilled by hosting master and doctoral students, and also by supporting advanced hands-on courses, namely one focused on cellular and molecular biology techniques within the Master in Marine Sciences – Marine Resources (Univ. Porto).


Highlights of main research achievements:

Line 1) Peroxisomes and related nuclear receptors (PPARs, RXRs, RARs) – Interplay mechanisms, endocrine modulation, and metabolic consequences of disruption

We continued to work on mechanisms inherent to our hypothesis and new discover that PPARs gene expression varies along the fish breeding cycle. We started working on molecular pathways impacts and metabolic consequences on lipid profiling. Moreover, we continued to offer insights after establishing that PPARα expression in females followed the same annual variation pattern as peroxisome size and enzyme activities, and an inverse pattern relatively to the salmonid type of annual plasma estradiol levels. The data agreed with the idea that PPARα is under estradiol modulation and that cross-talk between this receptor and the estrogen receptor exists. This has implications on fundamental biology and toxicology. In the same vein, we expanded the reported first sequence of the peroxisomal 17β-hydroxysteroid dehydrogenase 4 in fish, which was in accordance with a dual role for this enzyme in the liver: fatty acid and estradiol catabolism, throughout the annual breeding cycle of female trout. We also looked at the gene expression of a key peroxisomal enzyme (urate oxidase) and one crucial protein involved in peroxisome biogenesis (PEX11) with the trout model. In agreement to our data and hypothesis, the seasonal expression kinetics in females was closely related with key reproductive physiology events. All these regulatory aspects started to be tackled with in vitro assays. We managed to partially reproduce in primary liver cultures what is seen in vivo under estradiol influence: a decrease in the size of peroxisomes with parallel decreases of expression of some peroxissomal-related genes. Other insights of modeling of PPARα and other genes involved on lipid metabolism were also explored in a commercially important fish, the turbot Scophthalmus maximus. Further, on a phylogenetic perspective, we initiated an exhaustive search of Nuclear Receptor (NR) on publish and unpublished genomes to determine the NR complement in invertebrates. The analysis has been complemented with phylogenetic analysis, generating a comprehensive context of NR diversity in invertebrate genomes. Some experimental work has begun with the isolation of PPAR and RAR orthologues in molluscs and echinoderms, for future toxicology studies.

Line 2) Toxicopathology, Toxicogenomics and Chemical Toxicology

We continue to publish new chemical surveys for xenoestrogenic endocrine disrupting compounds (EDCs) and diverse pharmaceuticals in Portugal, covering estuaries like the Sado, Mondego, Douro or the Ave. The approach integrated seasonal and spatial distributions. We continued to cover EDCs either of animal (estradiol and estrone), vegetal (daidzein, genistein and biochanin A), pharmaceutical (17a-ethynylestradiol, propanolol, antibiotics, among other)


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or industrial (alkylphenols) origins. We recently enlarged of analytical portfolio of validated methods, eg, with a method covering dozens of pesticides. Several compounds existed in high amounts in water, and taking in account that these pollutants may additively contribute to endocrine disruption, we concluded that all estuaries had a biologically significant pollution by EDCs. Overall, almost all EDCs existed in all seasons and, therefore, might have been contributing to endocrine disruption, as revealed by the relatively high rate of ovotestis in some local fish (around 20% in mullets, by our most recent data). Also in the Douro, we published surveys for tackling a range of pharmaceuticals, using previously validated methodology. With embryo tests and experimental exposures we wanted to tackle whether or not the chemicals and concentrations that we are finding in the wild have biological impacts, namely on the gonad maturation kinetics and on the brain-hypophysis-gonad axis, combining quantitative histopathology with molecular and even behavioral approaches. For this we used several model organisms, such as the zebrafish. Finally, we published some data from an international partnership, tackling endocrine disruption from anti-androgens, using the frog Xenopus laevis.

Other studies) Lines 1 and 2 Related Contributions to Aquatic Biology

When studying the liver both for peroxisome biology and for toxicology, we continued to generate sound reference data that establishes ranges of normality for our model species, by using stereological approaches on both fish liver and kidney, and organs of mollusks (in the latter with emphasis on the phylogeny of the digestive tube of Opisthobranchia).

8d. Productivity


1. Behrends, T, Urbatzka, R, Krackow, S, Elepfandt, A, Kloas, W. 2010. Mate calling behavior of male South African clawed frogs (Xenopus laevis) is suppressed by the antiandrogenic endocrine disrupting compound flutamide. General and Comparative Endrocrinology 168: 269-274.

2. Hermelink, B, Urbatzka, R, Wiegand, C, Pflugmacher, S, Lutz, I, Kloas, W. 2010. Aqueous leaf extracts display endocrine activities in vitro and disrupt sexual differentiation of male Xenopus laevis tadpoles in vivo. General and Comparative Endocrinology 168: 245-255.

3. Lobo-da-Cunha, A, Oliveira, E, Alves, A, Coelho, R, Calado, G. 2010. Light and electron microscopic study of the anterior oesophagus of Bulla striata (Mollusca, Opisthobranchia). Acta Zoologica 91:125-138.

4. Lobo-da-Cunha, A, Oliveira, E, Ferreira, I, Coelho, R, Calado, G. 2010. Histochemical and ultrastructural characterization of the posterior esophagus of Bulla striata (Mollusca, Opisthobranchia). Microscopy and Microanalysis 16: 688-698.

5. Madureira, TV, Barreiro, JC, Rocha, MJ, Rocha, E, Cass, QB, Tiritan, ME. 2010. Spatio-temporal distribution of pharmaceuticals in the Douro River estuary (Portugal): A study using liquid chromatography ion trap mass spectrometry. Science of the Total Environment 408: 5513-5520.


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6. Madureira, TV, Rocha, MJ, Cass, QB, Tiritan, ME. 2010. Development and optimization of a HPLC-DAD method for the determination of diverse pharmaceuticals in estuarine surface waters. Journal of Chromatographic Science 48: 176-182.

7. Massari, A, Urbatzka, R, Cevasco, A, Canesi, L, Lanza, C, Scarabelli, L, Kloas, W, Mandich, A. 2010. Aromatase mRNA expression in the brain of adult Xenopus laevis exposed to Lambro river water and endocrine disrupting compounds. General and Comparative Endrocrinology 168: 262-268.

8. Nogueira, I, Lobo-da-Cunha, A, Afonso, A, Rivera, S, Azevedo, J, Monteiro, RAF, Cervantes, R, Gago-Martinez, A, Vasconcelos, V. 2010. Toxic effects of domoic acid in the seabream Sparus aurata. Marine Drugs 8: 2721 -2732.

9. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Franquinho, F, Monteiro, RAF, Rocha, E. 2010. Histological and stereological characterization of brown trout (Salmo trutta) trunk kidney. Microscopy and Microanalysis 16: 677-687.

10. Rocha, E, Rocha, MJ, Lobo-da-Cunha, A, Galante, MH, Monteiro, RAF. 2010. The hepatocytes of the brown trout (Salmo trutta f. fario): A stereological study of some cytoplasmic components during the breeding cycle. Microscopic Research & Technique 73: 766-778.

11. Urbatzka, R, Lorenz, C, Lutz, I, Kloas, W. 2010. Expression profiles of LH beta, FSH beta and their gonadal receptor mRNAs during sexual differentiation of Xenopus laevis tadpoles. General and Comparative Endocrinology 168: 239-244.

12. Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the myosin light chains 1, 2 and 3 in the muscle of blackspot seabream (Pagellus bogaraveo, Brunnich), during development. Fish Physiology and Biochemistry 36: 1125-1132.

In press

13. Rocha, MJ, Ribeiro, C, Ribeiro, M. (in press). Development and optimization of a GC-MS method to evaluate relevant endocrine disrupters in environmental matrices - estrogens and persistent pollutants. International Journal of Environmental Analytical Chemistry, doi: 10.1080/03067319.2010.496043.

14. Silva, P, Valente, LMP, Olmedo, M, Álvarez-Blázquez, B, Galante, MH, Monteiro, RAF, Rocha, E. (in press). Influence of temperature on muscle fibre hyperplasia and hypertrophy in larvae of blackspot seabream, Pagellus bogaraveo. Aquaculture Research, doi: 10.1111/j.1365-2109.2010.02627.x.

15. Lima, D, Reis-Henriques, MA, Silva, R, Santos, AI, Castro, FC, Santos, MM (in press). Tributyltin-induced imposex in marine gastropods involves tissue-specific modulation of the Retinoid X Receptor. Aquatic Toxicology, doi: 10.1016/j.aquatox.2010.09.022.


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1. Urbatzka, R, Rocha, MJ, Rocha, E. 2010. Regulation of Ovarian Development and Function in Teleosts. In: Norris, DO, Lopez, KH (Eds). Hormones and Reproduction in Vertebrates – Volume 1 Fishes, Academic Press, pp. 65-82. ISBN: 978-0-12-375009-9.

2. Wilson, JM, Castro, LFC. 2010. Morphological diversity of the gastrointestinal tract in fishes. In: Grosell, M, Farrell, AP, Brauner, CJ (Eds). The multifunctional gut of fish – Volume 30, Elsevier Inc., pp. 1-55. ISBN: 978-0-12-374982-6.

3. Rebok, K, Kostov, V, Rocha, E, Jordanova, M. 2010. Can rodlet cells change in barbell (Barbus peloponnesius) from the river Bregalnica be used as biomarkers of environmental contamination? Proceedings of the BALWOIS 2010 – Ohrid, Republic of Macedonia, pp. 1-7.

In press

4. Santos, MM, Reis-Henriques, MA, Castro, LFC (in press). Lipid homeostasis perturbation by organotins: effects on vertebrates and invertebrates. In: Pagliarani, A., Trombetti, F, Ventrella, V (Eds). Biochemical and biological effects of organotins, Chapter 6. Beham Science Publishers, Ltd.



MASTER THESES

Santos, Marta Susana Amaro. 2010. An histopathological and stereological study of proliferative hepatocellular lesions in a fish model of experimental carcinogenesis and in a sentinel fish. Master thesis in Oncology. ICBAS, University of Porto, Supervisor: Eduardo Rocha, Co-supervisor: Rogério A.F. Monteiro.

Pereira, Fernanda Cristina Rodrigues Malhão. 2010. Characterization of the trout liver cell line RTL-W1 and its potential for carcinogenesis-related studies. Master thesis in Oncology. ICBAS, University of Porto, Supervisor: Eduardo Rocha, Co-supervisor: Rogério AF Monteiro.

Silva, Rita Ribeiro. 2010. Hepatic regeneration and the role of platelets. Master thesis, Medicine, ICBAS, University of Porto, Supervisor: Eduardo Rocha.

PhD THESES

Ribeiro, Cláudia Maria Rosa. 2010. Chemical identification and monitoring of natural and xenoestrogens compounds in the estuaries of the estuaries of the Portuguese rivers Douro, Mondego and Sado – Insights on their disruption potential by in vitro exposures of testis from the model organism Nile Tilapia (Oreochromis niloticus). PhD Thesis in Biomedical Sciences,


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ICBAS, University of Porto. Supervisor: Maria João Rocha, Co-supervisors: Eduardo Rocha, Maria Elisabeth Tiritan.





International recognition by publishers and peers

- Team members served as peer-reviewers in indexed journals. The PI continued as Editor-in-Chief of Comparative Hepatology (BioMed Central - Springer). We published two invited book chapters for internationally renowned book houses.

Reinforcing scientific networking

- From our 17 international publications, we had 9 papers in with colleagues (at least one co-author) from abroad, so making 53% of our scientific output with international networking. Also, 47% of our scientific output had at least 1 author from other national group. All these figures materialized our tradition to consistently build collaborations.

- Collaborative projects continued in 2010, with research groups in Macedonia (Faculty of Natural Sciences and Mathematics, Univ. Sts. Cyril & Methodius), Japan (several partners), Norway (NTNU, Trondheim), UK (King’s Colleague and CEFAS), USA (Duke University), Germany (Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany), Thailand (Kasetsart University and adding King Mongkut’s University of Technology, Bangkok) and Federal University of São Carlos (Brazil).

International collaborative papers were:

- Behrends T, Urbatzka R, Krackow S, Elepfandt A, Kloas W. 2010. General and Comparative Endrocrinology 168: 269-274.

- Hermelink B, Urbatzka R, Wiegand C, Pflugmacher S, Lutz I, Kloas W. 2010. General and Comparative Endocrinology 168: 245-255.

- Madureira TV, Barreiro JC, Rocha MJ, Rocha E, Cass QB, Tiritan ME. 2010. Science of the Total Environment 408: 5513-5520.

- Madureira TV, Rocha MJ, Cass QB, Tiritan ME. 2010. Journal of Chromatographic Science 48: 176-182.


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- Massari A, Urbatzka R, Cevasco A, Canesi L, Lanza C, Scarabelli L, Kloas W, Mandich A. 2010. General and Comparative Endrocrinology 168: 262-268.

- Urbatzka R, Lorenz C, Lutz I, Kloas W. 2010. General and Comparative Endocrinology 168: 239-244.

- Rebok K, Kostov V, Rocha E, Jordanova M. 2010. Proceedings of the BALWOIS 2010 – Ohrid (Republic of Macedonia), pp. 1-7.



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Chemical Speciation and Bioavailability (CS&B)



Chemical Speciation and Bioavailability (CS&B)


Maria Teresa Sa Dias de Vasconcelos




In the year 2010 the CS&B Lab was involved in three financed projects/protocols at the National level and one at the International level as a coordinator (Portuguese part). National projects/protocols were financed by a private company (Aquaculture Company), by FCT (project PTDC/MAR/099140/2008) and by Rectorate of University of Porto /Santader Totta (Investigação Jovem, Univ Porto). The international project was financed by FCT and by the Spanish partner (project ERA-AMPERA/0003/2007). Another source of financing was the Pluriannual FCT funds attributed to PhD researchers and doctoral fees. Total funds involved in the mentioned period was ca. 50.000 EUR. A part of those funds (ca. 5 %) were obtained through contract-research activities (in cooperation with private companies), whereas the research project financed by FCT and project partner accounted for ca. 38 % and Pluriannual FCT funds and doctoral fees represented ca. 57 % of the total funds.


1. Objectives

Research carried out at CS&B Lab intends to give a contribution for the comprehensiveness of biogeochemical processes in aquatic environment and soil in order to be able to find remediation solutions when required. It may be also relevant for risk assessment purposes.

Main objectives for 2010 were as follows:

- To proceed the studies on mutual interactions among phytoplankton (mainly cyanobacteria) and emerging contaminants (endocrine disrupters and pharmaceutical compounds);

- To proceed the studies on the suitability of vascular plants for application in technologies of remediation, namely of metals and organic contaminants, like


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pesticides, hydrocarbons or organotins, giving emphasis on the plant role in conditioning the nearby environment. The interactions between plants and the microbial community in its rhizosphere were also accounted for. Included in this topic was the investigation on suitable biological remediation processes for the reduction/elimination of residues of commonly used pharmaceuticals compounds which are released by waste water treatment plants (WWTP) into the aquatic environment;

- To proceed investigation on bioremediation of beaches contaminated with petroleum hydrocarbons;

- To proceed the research on the monitorization of contamination by inorganic and organic pollutants, namely metals and PAHs, using marine organisms as bioindicators.

These studies included financed projects in progress, collaboration protocols, Post Doc, PhD, MSc and under-graduation projects.


Studies on mutual interactions of phytoplankton and compounds with endocrine disrupting effects and pharmaceutics are in progress, anchored by a Post Doc, a PhD project and an under-graduation project. One paper was published, two are accepted and another is in preparation. A Post Doc project on cyanobacteria toxins, carried out in collaboration with CIIMAR LEGE Lab, also fits this research line, with one paper in preparation. The line focused on mutual influence of metallic species and cyanobacteria resulted on one submitted paper.

Studies regarding optimization of analytical methods for determination of veterinary pharmaceuticals were carried out under a PhD and a Post Doc projects and two papers are submitted.

During 2010 the PhD project on the theme: “the barnacle as bioindicator of metals and polycyclic aromatic hydrocarbons contamination in the Portuguese coast” continued. The main objectives of this research are to optimize the analytical methods to measure metals and organic pollutants in biological marine matrices using the experience of the laboratory; to find out whether the barnacles in the northern Portuguese coast can be used as bioindicators of these chemical compounds in the marine environment; what levels of metals and organic compounds can be accumulated in these organisms and if those levels influence barnacles distribution on the coast. One research paper has been accepted for publication at an international scientific journal and two others have been submitted. Some of the results were also presented as a poster at a national Congress. Water quality analysis of aquaculture enterprises are also being carried out and knowledge and experience in quality assurance and control is being achieved.

Studies on the role of vascular plants in conditioning the nearby environment and plant capability for rhizoremediation (direct plant action combined with microbial role) continued with two papers published. This topic was intensified in the ambit of PhD project and a national project, both in collaboration with CIIMAR Hydrobiology Lab, with three papers already accepted for publication and in preparation. Optimization of methodologies for detection and quantification of compounds released by vascular plants into the medium (exudates) were carried out with success, in the ambit of a PhD project and a paper with the results is in


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preparation. Under this PhD projects several studies on the the effects compounds exudated by plants were carried out.

The investigation of suitability of biological processes for remediation of a soil contaminated with petroleum hydrocarbons (PHC) were concluded (in the ambit of a finished PhD, MSc and under-graduated projects) main results being obtained on optimization of expeditious methods to estimate PHC degradation extent, role/evolution of microbial community and plant influence on PHC degradation. Four articles and one book chapter are submitted on this topic. The research, in collaboration with CIIMAR Hydrobiology Lab (an international interdisciplinary project), concerning PHC bioremediation in contaminated beaches continued, involving an under-graduation and two MSc projects.

Results of the different studies were presented as oral or poster communications, in nine National and International Conferences.

8d. Productivity


1. Carvalho, PN, Basto, MCP, Silva, MFGM, Machado, A, Bordalo, A, Vasconcelos, MTSD. 2010. Ability of salt marsh plants for TBT remediation in sediments. Environmental Science and Pollution Research 17: 1279-1286.

2. Couto, MNPFS, Monteiro, E, Vasconcelos, MTSD. 2010. Mesocosms trials of bioremediation of contaminated soil of a petroleum refinery: comparison of natural attenuation, biostimulation and bioaugmentation. Environmental Science and Pollution Research 17: 1339-1346.

3. Mucha, AP, Almeida, CMR, Bordalo, A, Vasconcelos, MTSD. 2010. LMWOA (low molecular weight organic acid) exudation by salt marsh plants: Natural variation and response to Cu contamination. Estuarine, Coastal and Shelf Science 88: 63-70.

4. Stoichev, T, Basto, MCP, Vasconcelos, V, Vasconcelos, MTSD. 2010. Fate and effects of nonylphenol in the presence of the cyanobacterium Microcystis aeruginosa. Chemistry and Ecology 26: 395-399.

In press

5. Morais, P de, Stoichev, T, Basto, MCP, Carvalho, PN, Vasconcelos, MT. (in press). A headspace SPME-GC-ECD method suitable for determination of chlorophenols in water samples. Analytical and Bioanalytical Chemistry, doi: 10.1007/s00216-010-4610-y.

6. Mucha, AP, Almeida, CMR, Magalhães, CM, Vasconcelos, MTSD, Bordalo, A. (in press). Salt marsh plant-microorganism interaction in the presence of mixed contamination. International Biodeterioration & Biodegradation, doi: 10.1016/j.ibiod.2010.12.005.


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7. Ribeiro, H, Mucha, AP, Almeida, CMR, Bordalo, AA. (in press). Hydrocarbon degradation potential of salt marsh plant-microorganisms associations. Biodegradation, doi: 10.1007/s10532-010-9446-9.

8. Stoichev, T, Baptista, MS, Basto, MCP, Vasconcelos, VM, Vasconcelos MTSD. (in press). Fate and effects of minocycline and its degradation products in a Microcystis aeruginosa culture medium. Ecotoxicology and Environmental Safety, doi:10.1016/j.ecoenv.2010.10.015.




MASTER THESES

Pinto, Dorabela SSP. 2010. Avaliação da eficiência de remediação biológica (em termos microbiológicos e fitorremediação) de hidrocarbonetos de petróleo num solo contaminado. Master thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto.

Guedes, Paula ARA. 2010. Avaliação de estratégias de biorremediação para áreas afectadas por derrames de petróleo. Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto, Co-supervisor: C Marisa Almeida

PhD THESES

Couto, Maria Nazaré PFS. 2010. Investigation of physical, chemical and biological factors implicated in the biological remediation of nonvolatile petroleum hydrocarbons. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto.




Cooperation with:


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- Real Companhia Velha on Characterization of Port Wine

- SMGP – Consultores, Lda on chemical characterization of occupational environment

- Paracélsia Industria Farmacêutica S.A. on aluminum determination on serum samples

- Hovione FarmaCiencia SA on survey of some pharmaceutics effects on freshwater cyanobacteria. Does Good Manufacturing Practice (GMP) during pharmaceutics manufacture influence the biological response?

- Department of Production and Systems, School of Engineering, University of Minho

- Small and Media Companies, like Sondar Company and ECOINSIDE - Soluções em Ecoeficiência e Sustentabilidade, Lda., and Centres, like Centro de Apoio Tecnologia à Indústria Metalomecânica (CATIM), for training courses and projects for under-graduate and graduate students, namely for development of MSc theses.

- International programs of Quality Control: Lead in Blood, Instituto Nacional de Seguridad e Higiene en el Trabajo - INSHT, Zaragoza, Spain; Metals in Air, INSHT, Barcelona, Spain.


Research Collaboration

- Research collaboration with Marine and Environmental Geology group, University of Vigo, Spain (Prof. Ana Bernabeu) and Groupe d’Étude de l’Hydrodynamique et de la Dynamique Sédimentaire Littorale, University of Montpellier, France (Prof. Frédéric Bouchette) in the frame of the international project OILDEBEACH (an INTERREG project from the ERA-NET AMPERA).

Cooperation in International Organizations:

- International programs of Quality Control: Lead in Blood: Instituto Nacional de Seguridad e Higiene en el Trabajo (INSHT), Zaragoza; Metals in Air: INSHT, Barcelona.

Review of scientific papers:

- Senior CS&B members act very frequently as reviewers for several international scientific journals.



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Chemistry and Biological Activity of Marine Natural Products



Chemistry and Biological Activity of Marine Natural Products


Anake Kijjoa






FCT POCI/MAR/58114/2004 - Characterization and antitumor activity evaluation of the bioactive compounds from the marine sponges.

FCT PTDC/MAR/100482/2008 - Bioactive products in marine algae of Azores (AzoAlg) (approved budget 148.346 EUR).


1. Objectives

The objectives of the research group are:

- Search for bioactive secondary metabolites produced by terrestrial and marine derived macro- and microorganisms through collaborative bioprospecting programmes;

- Study of structural features of the isolated compounds by advanced spectroscopic techniques;

- Evaluation of biological and pharmacological activities of the crude extracts and pure compounds obtained from terrestrial and marine derived micro-and microorganisms;

- Evaluation of antitumor activity of secondary metabolites obtained from soil and marine-derived fungi as well as studying their mechanism underlying this activity;


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- Profiling the secondary metabolites for the chemosystematic evaluation.

- Profiling the secondary metabolites for the chemosystematic evaluation.


The main achievements of the group are:

- Isolation and characterization of three novel reverse prenylated indole alkaloids from the culture of soil fungus Neosartorya glabra with relevant antitumor activity;

- Isolation and characterization of the bioactive secondary metabolites from the culture of the sponge associated fungus Eurotium cristanum;

- Isolation and identification of novel secondary metabolites with unprecedented structural features from the cultures of soil and marine fungi.

Scientific output in publications

- The group has published so far around 100 peer reviewed papers in the field of bioactive natural products from terrestrial and marine sources.

Scientific networking and collaborations

- Although maintaining traditional collaborations with the Department of Chemistry and Biochemistry of the Florida State University (U.S.A), Institute of Marine Science of Burapha University (Thailand) and Faculty of Agriculture of Kasetsart University (Thailand), the group has started the research collaboration with Prof. Robert Kiss (Laboratory of Toxicology - Institute of Pharmacy - Free University of Brussels) as well as with Prof. Peter Proksch (Institute of Pharmaceutical Biology and Biotechnology, University of Düsseldorf, Germany).

International recognition by publishing houses and editors

- The number and quality of scientific publications of the principal investigator (Anake Kijjoa) has been recognized internationally. This is reflected by the participation of Anake Kijjoa as a member of Editorial Advisory Board of the scientific journals Marine Drugs and Journal of Natural Pharmaceuticals. Anake Kijjoa is also a regular reviewer of the manuscripts submitted for publication in various journals such as Chemistry and Physics of Lipid, Journal of Natural Products, Marine Drugs, Pharmaceutical Biology.

- The international recognition of the work of Anake Kijjoa in the field of Marine Natural Products has resulted in the organization of the 6th European Conference on Marine Natural Product which took place in Porto, Portugal, 19-23 July 2009 and his appointment as a member of the International Advisory Board in the 7th European Conference on Marine Natural Product which will take place in Strömstad, Sweden, 14-18 August 20011. Besides, Anake Kijjoa was appointed as a member of the Marine Board Working Group in Marine Biotechnology of the European Scientific Foundation. As a member of this working group, he was invited to coordinate the topics of


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“Development of Novel Pharmaceutical Products” and “Biodiscovery/Bioscreening for Novel drugs, compounds and biomaterials” for the Position Paper of this group which is published by the Marine Biotechnology Board in 2010.

8d. Productivity


1. Almeida, AP, Dethoup, T, Singburaudom, N, Lima, R, Vasconcelos, MH, Madalena, M, Kijjoa, A. 2010. The in vitro anticancer activity of the crude extract of the sponge-associated fungus Eurotium cristanum and its secondary metabolites. Journal of Natural Pharmaceuticals 1: 25-29.


1. Kijjoa A. (Guest Editor). 2010. Special Issue: Marine Natural Products, 6th European Conference on Marine Natural Products, Porto, 19-23 July 2009. Phytochemistry Reviews 9(4).

2. Auamcharoen, W, Chandrapatya, A, Naengchomnong, W, Kijjoa, A. 2010. A new tetralone from Diospyros cauliflora. Planta Medica 76: 1207.

3. Queiroz,CA, Kijjoa, A, David, JM, David, JP. Flavonoids and benzoic acid derivatives from Dioclea virgata. Planta Medica 76: 1284.

4. Bǿrrensen, T, Boyen, C, Dobson, A, Höfle, M, Ianora, A, Jaspars, M, Kijjoa, A, Olafsen, J, Querellou, J, Rigos, G, Wijffels, R. 2010. Marine Biotechnology: A new vision and strategy for Europe. Marine Board-ESF Position Paper 15: 1-91.







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Collaborative Research

- The group has collaboration in research work with the following international institutions: Laboratory of Toxicology - Institute of Pharmacy - Free University of Brussels (Prof. Robert Kiss); Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Thailand (Prof. Leka Manoch); Department of Chemistry and Biochemistry, The Florida State University, Tallahassee, USA (Professor Werner Herz); Bangsaen Institute of Marine Science, Burapha University, Chonburi, Thailand (Dr. Sumaitt Putchakarn).

Graduate Training

- The laboratory of Natural Products Chemistry has received PhD’s students from Thailand and Brazil for the training on isolation, purification and structure elucidation of natural products, under the network of mobility programme.

- ERASMUS Programme with the Faculty of Pharmacy, Ankara University, Turkey.

Networking

- ASEM-DUO Network with Burapha University, Bangkok, Thailand.

- A Kijjoa is a member of Marine Board Working Group in Marine Biotechnology. He is coordinating the topics of “Development of Novel Pharmaceutical Products” and “Biodiscovery/Bioscreening for Novel drugs, compounds and biomaterials” for the Position Paper of this group which will be published by the Marine Biotechnology Board in 2010.

Other

- A Kijjoa is a member of Editorial Advisory Board of the Following Journals: Marine Drugs, Journal of Natural Pharmaceuticals.

- A Kijjoa is a reviewer of the following scientific journals: Chemistry and Physics of Lipids, Journal of Natural Products, Marine Drugs, Pharmaceutical Biology.

- A Kijjoa was also a guest editor of the Special Issue: Marine Natural Products (6th European Conference on Marine Natural Products, Porto, 19-23 July 2009). Phytochemistry Reviews, Volume 9, Number 4, 2010.


A Kijjoa is a member of Marine Board Working Group in Marine Biotechnology. He is coordinating the topics of “Development of Novel Pharmaceutical Products” and “Biodiscovery/Bioscreening for Novel drugs, compounds and biomaterials” for the Position Paper of this group which will be published by the Marine Biotechnology Board in 2010.


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Coastal Biodiversity



Coastal Biodiversity


Isabel Maria Trigueiros Sousa Pinto Machado




- 30.000 EUR FCT pluriannual funding

- FCT, 2010-2013 (about 1 M Euros)

121.000 EUR, BENEFITS - Benefits of production of seaweeds in multitrophic aquaculture systems

117.000 EUR, PHYSIOGRAPHY - Physiological stress of intertidal fucoids related to their biogeography

68.000 EUR, INVASEA - Shifts from seagrass to seaweed dominated system

35.000 EUR, CARRAGEENAN - Microstructure and the mechanical properties in carrageenan gels

70.000 EUR, CleF - The combined impacts of invasion and climate change on coastal ecosystem functioning

54.000 EUR, PhD and Post-doc grants

- FP6 and FP7, EC

203.000 EUR, Era-Net BIODIVERSA, 2006-2010

148.000 EUR, BIOSTRAT - Developing the EU Biodiversity Research Strategy, 2006-2010

80.000 EUR, KNEU, 2010-2013

- Others

24.000 EUR, Quanternaire


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100.000 EUR, QREN, IBEROMARE Centro Multipolar de Valorização de Recursos Marinhos, 2009-2011

58.000 EUR, QREN, EEMA Avaliação do Estado Ecológicos das Massas de Água Costeiras, 2009-2011

57.000, Axa Foundation, Marine Aliens and Climate Change, 2009-2011

220.000, Polis Litoral, CHRACTER, 2010-2012

- Approved to start in 2011 (about 0.5 M Euros)

62.000 EUR, FP7, Euromarine

265.000 EUR, FP7, Seaweed Star

63.336 EUR, FCT, GreenMount

20.000 EUR, FCT, RAP

50.000 EUR, FCT, OCEANKELP

135.000 EUR, Norwegian Research Council, SeaweedTech and Seabreed


1. Objectives

The general objectives of the group are:

- To increase knowledge and understanding of the different levels of organization of marine biodiversity (from organisms, populations, communities and ecosystems), its importance for the functioning of the living world and the consequences of anthropogenic pressures, particularly under current and predicted scenarios of global environmental changes.

- To increase our knowledge on a sustainable use of marine organisms, in order to produce or improve economic resources without endangering the functioning of marine ecosystems and the services they provide.

- To increase collaboration with companies and technological centers to promote economic activities that use the sea and its resources in a sustainable way, furthering the knowledge and expertise of our group within this interdisciplinary and multicultural environment, while helping the development of new products and services.

- To create an awareness of general public and young people in particular about the importance of biodiversity and of its role on the functioning of ecosystems and their services and the need to preserve it.


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- To strengthen reciprocal links between researchers and policy makers and managers, especially those responsible for marine and coastal areas and resources, in order to promote policy relevant research and to increase the contribution of scientific results to political decisions and management.

Within this framework the specific objectives of our group are:

- To determine the patterns of distribution and abundance of species on benthic communities and their variation at different spatial and temporal scales, across their geographic range of distribution in European waters, with special focus on communities along the Portuguese coast.

- To assess the effects of different stressors on coastal biodiversity, detected from the level of individual physiological responses to the functional effects at the ecosystem level, and using different approaches: from ecophysiological measurements to observational studies, demographic modelling and experimental manipulations of assemblages.

- To evaluate the structure and dynamics of biotic interactions and food webs in coastal systems, through the use of traditional and stable isotope signatures methods, and compare this with processes occurring in shallow seamounts.

- To develop hydrodynamic models for our coast with integration of physical, geochemical and biological data to understand fluxes nutrient and CO2 from land and oceanic origin and how they influence coastal productivity.

- To determine the role of coastal ecosystems as a CO2 sinks and how this role may be affected by global warming.

- To evaluate the effectiveness of large scale mariculture of macroalgae as CO2 sink and for production of biofuel, and other products as protein for feed and ashes for fertilizers together with companies operating at international level in aquaculture, marine transport, energy and new materials for cultivation.

- To evaluate the use of macroalgae as water purifiers and as an aquaculture product through the implementation of Integrated Multitrophic Aquaculture systems (IMTAs), in collaboration with existing commercial fish farms.

- To develop new products from different marine organisms, and increase the value of species used to improve water quality (IMTA systems) or discard organisms.

- To increase knowledge of the impacts of traditional and local fisheries and harvest of marine animals and algae on these resources and on the ecosystems that support them.

Besides these research objectives we also aim at:

- Producing outreach programs and materials for the general public and school children and improve public awareness and understanding of links and specificity of taxonomy,


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ecology and evolution, and of the increasing need and urgency for applying a scientific approach integrating these disciplines to coastal management and nature conservation.

- Promoting research that is relevant to the conservation and sustainable use of marine biodiversity, especially under a climate change context.

- Improving the effectiveness of communication and cooperation between researchers and policy makers in order to implement scientifically-based policies and action plans, at regional, National, European and Global level, including adaptation and mitigation strategies under climate change and biological invasions scenarios. This goal is strengthened through the development of programs for environmental characterization and long term data acquisition, in cooperation with and funding by regional and european institutions.


Our lab was the result of an internal re-organization that broke the Laboratory of Biodiversity of Aquatic Ecosystems in 4 labs. During 2010 we published 31 papers, including 17 in ISI journals, and 6 books or book chapters and submitted several more.

Other main achievement during 2010 included:

- Determination of seasonal patterns of tidepool macroalgae communities in two north Portugal beaches and the spatial variability of intertidal macroalgal communities along the North Portuguese coast.

- Determination of the interactive effects of environmental stressors, namely air and water temperature on the metabolism and growth of key intertidal seaweeds like several species of Fucus and Hymanthalia.

- Investigations on the interactive effects of climate stressors (pCO2 and water temperature increases) and invasions on the functioning of macroalgal communities.

- Start of research on new invaders to our seashores (i.e. Xenostrobus securis and Undaria pinnatifida).

- Tests on the effect of native predator (Carcinus maenas) in the invasivility of the non-indigenous mussel (Xenostrobus securis)

- Implementation of an hydrodynamic models for the Minho estuary

- Characterization of the productivity and capacity for nutrient uptake of different seaweeds including Martocarpus stellatus and Porphyra dioica, in IMTA.

- Testing selected seaweed species grown in IMTA systems as ingredients for fish feed and as source for compounds with antibiotic activity.


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We leaded and participate in several European, bilateral and National projects that were funded and will start in 2011 including:

- Greenmount: “Seamount benthic primary production: a new hypothesis to explain abundance and biodiversity over shallow seamounts” funded by FCT to continue our research on the structure and dynamics of biotic interactions and food webs in coastal systems, and compare this with processes occurring in shallow seamounts.

- RAP – “Responses to Anthropogenic Perturbations: climatic and nutrient effects on rock pool assemblages” and OCEANKELP “Effects of ocean climate on the macroecology and resilience to disturbances of kelp beds” funded by FCT that will enable us to develop more work in intertidal and on the kelp forests along the west coast of Portugal, understand the changes that have been observed and predict further changes as a result of climate changes.

- BIP “SeaweedTech - Development of large scale offshore seaweed cultivation” and BIP “Seabreed - Industrial seaweed seedling production for large scale offshore cultivation processs”, funded by the Nowergian Research Council and “Seaweed Star - Offshore cultivation of seaweed” funded by FP7. These projects will allow us to significantly further our work with (see below) to develop large scale mariculture of seaweeds to produce biofuels, having also as by-product protein that will be tested for fish feed and ash that will be tested as fertilizer.

- EUROMARINE – “Integration of European Marine Research Networks of Excellence” and EMBOS – “Pan-European Marine Biodiversity Observatory System” funded by FP7 to continue to work at European level to increase knowledge and monitoring of marine biodiversity.

For outreach and involvement of young people in research we continued our program “MOBIDIC: the school goes to the beach” for monitoring rocky shore communities with school children and their teachers, had several internships for high school students, held a summer program and participated in several science fairs.

For linking research with policy makers and managers and promoting policy relevant research, at the European level we continue to participate in the EPBRS, which met to discuss research priorities for Europe and organized the meeting in Brussels: “Positive Visions for Biodiversity” This meeting produced a list of priority actions and a community that will follow more initiatives in the following years, and started a new FP7 project: KNEU – “Developing a Knowledge Network on biodiversity”. At international level we participated in the meeting of the Convention for Biological Diversity in Nagoya, Japan integrating the national delegation (see more under internationalization).

At regional level, we started a project “Caracterização da actividade pesqueira costeira e dos seus impactes nos recursos e nas comunidades marinhas do Litoral Norte” funded by Polis Litoral Norte to characterize the traditional fisheries and intertital harvesting activities and their impact on the resources and on the coastal ecosystems of the North Coast of Portugal.


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8d. Productivity


1. Azevedo, IC, Bordalo, AA, Duarte, P. 2010. Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersion in the Douro estuary (Portugal). Water Research 44: 3133-3146.

2. Azevedo, IC, Duarte, P, Bordalo, AA. 2010. Temporal and spatial variability of phytoplankton photosynthetic characteristics in a southern European estuary (Douro, Portugal). Marine Ecology Progress Series 412: 29-44.

3. Bertocci, I., Arenas, F., Matias, M., Vaselli, S., Araújo, R., Abreu, H., Pereira, R., Vieira, R., Sousa-Pinto, I. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116.

4. Cabral, JP. 2010. La revista Broteria, los jesuitas naturalistas y Gonçalo Sampaio. Intercambio de plantas e ideas y el desarrollo de la botánica en Portugal. Asclepio - Revista de Historia de la Medicina y de la Ciencia 62(1): 61-92.

5. Cabral, JP. 2010. O depósito de conchas do sítio arqueológico das “Areias Altas” (Porto, Portugal). Estudo morfológico e morfométrico das conchas de moluscos. Férvedes 6: 73-82.

6. Cabral, JP. 2010. Can we use indoor fungi as bioindicators of indoor air quality? Historical perspectives and open questions. Science of the Total Environment 408: 4285-4295.

7. Cabral, JP. 2010. Water microbiology. Bacterial pathogens and water. International Journal of Environmental Research and Public Health 7: 3657-3703.

8. Duarte, P, Fernández-Reiriz, MJ, Filgueira, RA, Labarta, U. 2010. Modelling mussel growth in ecosystems with low suspended matter loads, Journal of Sea Research 64: 273-286.

9. Konar, B, Iken, K, Cruz-Motta, JJ, Benedetti-Cecchi, L, Knowlton, A, Pohle, G, Miloslavich, P, Edwards, M, Trott, T, Kimani, E, Riosmena-Rodriguez, R, Wong, M, Jenkins, S, Mead, A, Silva, A, Sousa Pinto, I, Shirayama, Y. 2010. Global patterns of macroalgal diversity and biomass in rocky nearshore environments. Plos One 5: 13195.

10. Moreira, J, Veiga, P, Rubal, M. 2010. First record of Pisione guanche (Polychaeta: Pisionidae) at the Azores Archipielago. Marine Biodiversity Records 3: 1-6.

11. Vandepitte, L, Vanhoorne, B, Kraberg, A, Anisimova, N, Antoniadou, C, Araújo, R, Bartsch, I, Beker, B, Benedetti-Cecchi, L, Bertocci, I, Cochrane, SJ, Cooper, KM, Craeymeersch, JA, Christou, E, Crisp, DJ, Dahle, S, De Boissier, M, de Kluijver, M, Denisenko, S, De Vito, D, Duineveld, G, Escaravage, V, Fleischer, D, Fraschetti, S, Giangrande, A, Heip, CHR, Hummel, H, Janas, U, Karez, R, Kedra, M, Kingston, P, Kuhlenkamp, R, Libes, M, Martens, P, Mees, J, Mieszkowska, N, Mudrak, S, Munda, I, Orfanidis, S, Orlando-Bonaca, M, Palerud, R, Rachor, E, Reichert, K, Rumohr, H, Schiedek, D, Schubert, P, Sistermans, WCH, Pinto, IS, Southward, AJ, Terlizzi, A, Tsiaga, E, van Beusekom, JEE. 2010. Data integration for European marine biodiversity research:


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creating a database on benthos and plankton to study large-scale patterns and long-term changes. Hydrobiologia 644: 1-13.

12. Veiga, P, Besteiro, C, Rubal, M. 2010. The role of sediment type on the vertical distribution of meiofauna at two Galician rias (NW Iberian Peninsula). Cahiers de Biologie Marine 51: 249-263.

13. Veiga, P, Besteiro, C, Rubal, M. 2010. Meiofauna communities in exposed sandy beaches on the Galician coast (NW Spain), six months after the Prestige oil spill: the role of polycyclic aromatic hydrocarbons (PAHs). Scientia Marina 74: 385-394.

In press

14. Abreu, MH, Pereira, R, Yarish, C, Buschmann, AH, Sousa-Pinto, I. (in press). IMTA with Gracilaria vermiculophylla: Productivity and nutrient removal performance of the seaweed in a land-based pilot scale system. Aquaculture, doi: 10.1016/j.aquaculture.2010.12.036.

15. Araújo, R, Violante, J, Pereira, R, Abreu, H, Arenas, F, Sousa-Pinto, I (in press). The recently introduced seaweed Grateloupia turuturu on the Portuguese coast: distribution and population dynamics. Phycologia.

16. Araújo, R, Serrão, EA, Sousa-Pinto, I, Ǻberg, P (in press). Phenotypic differentiation at southern limit borders: the case-study of two fucoid macroalgal species with different life-history traits. Journal of Phycology.

17. Veiga, P, Besteiro, C, Rubal, M. (in press). Meiofauna communities structure at different spatial scales on sandy beaches from a ria environment. The role of abiotic factors. Marine Biology Research, doi: 10.1080/17451000.2010.527987.


1. Araújo, R, Cabral, JP. 2010. Fungal air quality in medical protected environments. In: Kumar, A (Ed). Air Quality, InTech. ISBN: 978-953-307-131-2.

2. Carvalho, AR, Barros, N, Duarte, P. 2010. Impact of Oporto Metropolitan Area Carbon Dioxide Emissions over the Adjacent Coastal Zone. In: Duarte, P, Santana-Casiano, JM (Eds). Oceans and the atmospheric carbon content, Springer, pp. 23-46. ISBN 978-90-481-9820-7.

3. Duarte, P, Santana-Casiano, JM (Eds.) 2010. Oceans and the Atmospheric Carbon Content. Springer, 176 pp. ISBN 978-90-481-9820-7.

4. Elmqvist, TE, Maltby, E, Barker, Las, Mortimer, CAs, Aronson, J, De Groot, R, Fitter, A, Mace, G, Norberg, J, Perrings, C, Sousa Pinto, I, Ring, I. 2010. Biodiversity, ecosystems and ecosystem services. In: Pushpam, K. (Ed). The Economics of Ecosystems and Biodiversity: Ecological and Economic Foundations, Earthscan, London, UK, pp. 41-111. ISBN 978-18-497-1212-5.

5. Pereira, R, Yarish, C. 2010. The role of Porphyra in sustainable culture systems: Physiology and applications. In: Israel, A, Einav, R (Eds). Role of Seaweeds in a Globally Changing Environment, Springer Publishers, pp. 339-353.


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1. Arenas, F. 2010. Macroalgal marine invasions: Some highlights and knowledge gaps. Algas 43: 16-19.

2. Cabral, JP. 2010. Gonçalo Sampaio e a história da botânica. Memórias da Sociedade Broteriana, volume XXXIV, Sociedade Broteriana, Coimbra, Portugal.

3. Sousa Pinto, I. 2010. 2010 – Ano Internacional da Biodiversidade. Proceedings of the XI Jornadas Sobre Conservação da Natureza e Educação Ambiental: Educação para a Sustentabilidade. 8-9 Maio, Caminha, Portugal.


MASTER THESES

Domingues-Santos, Bernardo. 2010. Bioremediation efficiency of Mastocarpus stellatus (Stackhouse) Guiry, in an Integrated Multi-Trophic Aquaculture System. Master in Aquaculture, Interuniversity UB-UAB-UPC. Supervisor: Isabel Sousa Pinto, Co-supervisor: Lourdes Reig (UPC).

Silva, Diogo. 2010. Discrimination of Trisopterus luscus (Linnaeus 1758) stocks in the northern of Portugal using otolith elemental fingerprints. Master thesis in Sea Sciences - Marine Resources, ICBAS, University of Porto. Supervisor: Alberto Correia, Co-supervisor: Paulo Santos.

Pimenta, Pedro. 2010. Biodiversity in Serra da Aboboreira, NO Portugal. Master thesis, University of Porto. Supervisor: Paulo Santos.

Carvalho, André. 2010. Macrobenthos Biodiversity in Cávado estuary. Master thesis, University of Porto. Supervisor: Paulo Santos

Viegas, Sofia. 2010. Development of a more objective index for the quantification of environmental impacts. Master thesis, University of Porto. Supervisor: Paulo Santos, Co-supervisor: Sílvia Mesquita.

PhD THESES

Pereira, A. 2010. Desenvolvimento de um sistema integrado de modelação, calibração e gestão de ecossistemas costeiros, com recurso a agentes inteligentes. PhD in Electronic Engineering and Computers, Faculty of Engineering, University of Porto. Supervisor: Pedro Duarte.



Workshop “O Mar Aqui Tão Perto I”, Ocupação Científica dos Jovens nas Férias, Ciência Viva, 28 June - 2 July 2010, Porto, Portugal. I Sousa Pinto, R Vieira, M Pereira Silva - Organizers.


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Workshop “O Mar Aqui Tão Perto II”, Ocupação Científica dos Jovens nas Férias, Ciência Viva, 26-30 July 2010, Porto, Portugal. I Sousa Pinto, R Vieira, M Pereira Silva - Organizers.


During 2010 we increased significantly our work with private companies. We finished a contract with Quaternaire Portugal – Consultoria p/ o Desenvolvimento, S.A. for the characterization of coastal ecosystems and a management plan for biological resources and nature conservation for the POOC (Plan for Management of the Coastal Area) of Pico Island, Azores.

Besides this we signed 3 contracts of research with companies that will start in 2011.

BIP “SeaweedTech - Development of large scale offshore seaweed cultivation” and BIP “Seabreed - Industrial seaweed seedling production for large scale offshore cultivation processs”, and “Seaweed Star - Offshore cultivation of Seaweed”. These project will be develop with companies as Seaweed Energy Solutions, Aqualine Lda, Stolt Sea Farm AS, Winds Enterprise and Statoil, as well as SINTEF Fisheries and aquaculture and NTNU from Norway to develop large scale mariculture of seaweeds to produce biofuels, having also as by-product protein that will be tested for fish feed and ash that will be tested as fertilizer.


A high percentage of the work of our group is done within European and International collaborations. In 2010 we participated in 3 project from 6 and 7th Framework Programs EC, with researcher from about 70 European institutions, we are about to start 3 more and wrote and presented 3 other that are under evaluation. Our publications reflect this international involvement (see list). Furthermore Isabel Sousa Pinto is member of the Steering or Executive Committees of several European research organizations as: a) European Platform for Biodiversity Research Strategy (EPBRS), b) “Census of Marine Life” (European Section), c) MARBEF+ Marine Biodiversity and Ecosystem Functioning”, d) MARS – European Network of Marine Research Institutes and Stations, e) Euromarine: Integrating the NoEs on Marine Biodiversity and f) EMBOS Pan-European Marine Biodiversity Observatory System. She is also member of a) the “Study Group on Biodiversity Science” from ICES (International Council for the Exploration of the Sea), b) Working Group 5 – “Marine Ecosystem Change” from GEO BON (Group on Earth Observation – Global Biodiversity Observation Network) c) “Policy Interface Panel” from MARCOM+: Integrating Marine and Maritime Science Communities and d) editorial Board of the journal “Marine Biodiversity” edited by Springer, Germany.

As part of EPBRS work we were involved in the organization of the meeting in Brussels: “Positive Visions for Biodiversity” that brought together 250 people from very different backgrounds from around the world to discuss the vision for the future for the living world and how to get to this vision. www.biodiversity.be/epbrsbe2010

We started a new FP7 project: KNEU – “Developing a Knowledge Network on biodiversity” that aims at creating and testing a prototype scientific panel at European level to give inputs to IPBES: Intergovernamental Science Policy Panel on Biodiversity and Ecosystem Services created by the United Nations in 2010.

We started to work with several international companies (see Industry Contract Research).

http://www.biodiversity.be/epbrsbe2010


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We continued to offer the course “Monitoring of Rocky Shores” for the Erasmus Mundus Master program “Marine Biodiversity and Conservation” (EMBC) and welcomed 20 students from different countries in April and participated in the development of the Erasmus Mundus Doctoral Program MARES.


2009/2010 – Isabel Sousa Pinto was the Portuguese focal point for the study: "The impact of European policy on the development of the European Research Area (ERA)” in the areas relevant to the Environment (including climate change)".

Isabel Sousa Pinto participated in the Convention for Biological Diversity in Nagoya, Japan integrating the National Delegation by indication of the Ministry of Science Technology and Higher Education (MCTES), and being responsible to follow the working groups on Biodiversity and Climate Change, Marine and Coastal Biodiversity, Relations between the Conventions and the Inter-Governmental Panel on Biodiversity and Ecosystem Services (IPBES). This Convention approved a new strategic plan for 2011-2020 and the Nagoya Protocol to regulate the access and use of genetic resources and welcomed the launch of IPBES a scientific panel equivalent to IPCC for Biodiversity.

Our lab started the project Character “Caracterização da actividade pesqueira costeira e dos seus impactes nos recursos e nas comunidades marinhas do Litoral Norte” funded by Polis Litoral Norte, a public society for requalification and valorisation of the north coast of Portugal. The main objectives are the characterization of the main marine coastal habitats, the identification of the main species present in these habitats and its abundance. We will also characterize the traditional fisheries and intertital harvesting activities and their impact on the resources and on the coastal ecosystems of the North Coast of Portugal. The data obtained will be used for the elaboration of proposals for the sustainability of the traditional fishing in the northern coast of Portugal. The project was first presented during “Encontros do Litoral: Litoral Norte – Galiza” held in Viana do Castelo (Portugal) from 24 to 25 November 2010.


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Comparative Molecular Endocrinology (CME)



Comparative Molecular Endocrinology (CME)


Adelino Vicente Mendonca Canario





- FCT Pluriannual funding.

- National funding

25.695 EUR, FCT, PTDC/CVT/66735/2006, PTHL.

23.369 EUR, FCT, PTDC/MAR/69749/2006, SALARIA.

73.885 EUR, FCT, PTDC/MAR/104008/2008, BICA.

68.043 EUR, FCT, PTDC/CVT/104750/2008.

- European funding

112.243 EUR, European Commission, Contract Nr. 222719, LIFECYCLE.

35.034 EUR, European Commission, Contract Nr. 227799, ASSEMBLE.

11.102 EUR, Interreg IV – Atlantic Area, 2009-1/127, SHAREBIOTECH.

12.128 EUR, European Commission, COST ES0906.


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1. Objectives

The main aim of the group is to study the role of hormones and their metabolites on the physiology and the evolution of the endocrine system, using fish as the principal model and as required other invertebrate and vertebrate models. The research is directed at four main interrelated themes: 1) Hormonal control of Reproduction and Development; 2) Calcitropic hormones, mineral metabolism and environmental adaptation, 3) Hormones, pheromones and behaviour, 4) The impact of genotype on phenotype. Specific objectives are:

1) Hormonal control of reproduction and development

- To establish the identity, function and mechanisms of action of key regulatory factors in relation to sex differentiation, puberty and gonad maturation.

- To understand the process of metamorphosis and skeletal development in fishes, and the role of thyroid and metabolic hormones.

2) Calcitropic hormones, mineral metabolism and environmental adaptation

- To understand the evolution of mineral homeostasis and the role of endocrine regulating factors, their site of action and the molecular mechanisms responsible for their biological actions.

- To understand the process of ossification and regeneration in fishes and the role of the extracellular matrix, minerals and endocrine factors.

3) Hormones, pheromones and behaviour

- To understand the underlying mechanisms regulating the interaction between hormones and behaviour in fishes.

- To understand how fish detect biologically important environmental chemical cues - pheromones, food-related odorants and inorganic cations (Ca2+ and Na+) and how this olfactory input is centrally processed to evoke the appropriate physiological and behavioural responses.

4) The impact of genotype on phenotype

- To apply an integrative and systems approach, from the genome to individuals and populations, to the study of the evolution of endocrine hormones/systems and their impact on phenotype.


Scientific discoveries:


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- We have established the basis for characterization of the endocrine regulation of bicarbonate secretion in the intestinal epithelium of marine fish; its relationship with calcium transport and the potential importance of calcitropic hormones in ion regulation at whole body level.

- Used new generation sequencing and serial analysis of gene expression to produce the gill transcriptome and how it changed in response to calcium changes.

- Established PTHrP as a regulator of phosphate in fish kidney.

- Discovered with colleagues from the University of Madeira that the female:male sex ratio of loggerhead turtles around Madeira is much lower than that of young when leaving the nesting beaches, which is indicative of differential mortality.

- Collaborated with colleagues from Bangor University in the discovery of unsuspected diversity of nematode species in benthic habitat. The methodology used is proof of concept of allying new generation sequencing to PCR and bioinformatics.

- Identified a novel cartilage acidic protein in fishes homologues of which are present in many organisms from bacteria to human. The protein is normally extracellular and in fish is also secreted by the pituitary.

- Identified with European colleagues potential quantitative trait loci for the cortisol response, as well as for growth and somatic characters in sea bass.

Technology development /transfer:

- Developed with colleagues from the Roscoff Marine Station of a high-throughput method to produce recombinant proteins which can be used for crystallization and structural analysis and other studies.

- Accomplished with colleagues from the Max Plank Institute of Molecular Biology a draft sequence of the European sea bass genome, a basic resource for the scientific community and aquaculture industry.

8d. Productivity


1. Campinho, MA, Galay-Burgos, M, Sweeney, GE, Power, DM. 2010. Coordination of deiodinase and thyroid hormone receptor expression during the larval to juvenile transition in sea bream (Sparus aurata, Linnaeus). General and Comparative Endocrinology 165(2): 181-194.

2. Cardoso, JCR, Vieira, FA, Gomes, AS, Power, DM. 2010. The serendipitous origin of chordate secretin peptide family members. BMC Evolutionary Biology 10: 135.


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3. Clark, MS, Thorne, MAS, Vieira, FA, Cardoso, JCR, Power, DM, Peck, LS. 2010. Insights into shell deposition in the Antarctic bivalve Laternula elliptica: gene discovery in the mantle transcriptome using 454 pyrosequencing. BMC Genomics 11: 362.

4. Creer, S, Fonseca, VG, Porazinska, DL, Giblin-Davis, RM, Sung, W, Power, DM, Packer, M, Carvalho, GR, Blaxter, ML, Lambshead, PJD, Thomas, WK. 2010. Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises. Molecular Ecology 19: 4-20.

5. Delgado, C, Canário, AVM, Dellinger, T. 2010. Sex ratios of loggerhead sea turtles Caretta caretta during the juvenile pelagic stage. Marine Biology 157: 979-990.

6. Ferraresso, S, Milan, M, Pellizzari, C, Vitulo, N, Reinhardt, R, Canario, AVM, Patarnello, T, Bargelloni, L. 2010. Development of an oligo DNA microarray for the European sea bass and its application to expression profiling of jaw deformity. BMC Genomics 11: 354.

7. Fonseca, VG, Carvalho, GR, Sung, W, Johnson, HF, Power, DM, Neill, SP, Packer, M, Blaxter, ML, Lambshead, PJD, Thomas, WK, Creer, S. 2010. Second-generation environmental sequencing unmasks marine metazoan biodiversity. Nature Communications 1: 98.

8. Fuentes, J, Brinca, L, Guerreiro, PM, Power, DM. 2010. PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge. General and Comparative Endocrinology168(1): 95-102.

9. Fuentes, J, Power, DM, Canario, AV. 2010. Parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 299(1): R150-158.

10. Goncalves, D, Saraiva, J, Teles, M, Teodosio, R, Canario, AVM, Oliveira, RF. 2010. Brain aromatase mRNA expression in two populations of the peacock blenny Salaria pavo with divergent mating systems. Hormones and Behavior 57(2): 155-161.

11. Groisillier, A, Herve, C, Jeudy, A, Rebuffet, E, Pluchon, PF, Chevolot, Y, Flament, D, Geslin, C, Morgado, IM, Power, DM, Branno, M, Moreau, H, Michel, G, Boyen, C, Czjzek, M. 2010. MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms. Microbial Cell Factories 9: 45.

12. Guerreiro, PM, Canario, AVM, Power, DM, Renfro, JL. 2010. Piscine PTHrP regulation of calcium and phosphate transport in winter flounder renal proximal tubule primary cultures American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 299: R603-R611.

13. Huertas, M, Hagey, L, Hofmann, AF, Cerda, J, Canario, AVM, Hubbard, PC. 2010. Olfactory sensitivity to bile fluid and bile salts in the European eel (Anguilla anguilla), goldfish (Carassius auratus) and Mozambique tilapia (Oreochromis mossambicus) suggests a `broad range' sensitivity not confined to those produced by conspecifics alone. Journal of Experimental Biology 213(2): 308-317.


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14. Ibarz, A, Costa, R, Harrison, AP, Power, DM. 2010. Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach. General and Comparative Endocrinology 169(3): 284-292.

15. Kuhl, H, Beck, A, Wozniak, G, Canario, AVM, Volckaert, FAM, Reinhardt, R. 2010. The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing. BMC Genomics 11: 68.

16. Louro, B, Passos, ALS, Souche, E, Tsigenopoulos, C, Beck, A, Bonhomme, F, Cancela, L, Cerdà, J, Clark, MS, Kotoulas, G, Lubzens, E, Planas, J, Volckaert, FAM, Reinhardt, R, Canario, AVM. 2010. Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: characterization, tissue specific expression and gene markers. Marine Genomics 3(3-4): 179-191.

17. Massault, C, Hellemans, B, Louro, B, Batargias, C, Van Houdt, JKJ, Canario, AVM, Volckaert, FAM, Bovenhuis, H, Haley, C, de Koning, DJ. 2010. QTL for body weight, morphometric traits and stress response in European sea bass Dicentrarchus labrax. Animal Genetics 41(4): 337-345.

18. Pinheiro, PLC, Cardoso, JCR, Gomes, AS, Fuentes, J, Power, DM, Canário, AVM. 2010. Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken. BMC Evolutionary Biology 10: 373.

19. Pinto, PI, Matsumura, H, Thorne, MA, Power, DM, Terauchi, R, Reinhardt, R, Canario, AVM. 2010. Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish. BMC Genomics 11: 476.

20. Power, DM, Fuentes, J, Harrison, AP. 2010. A non-invasive monitoring device for anesthetics in fish. Open Access Animal Physiology 2: 1-7.

21. Redruello, B, Louro, B, Anjos, L, Silva, N, Greenwell, RS, Canario, AVM, Power, DM. 2010. CRTAC1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pituitary gland. Gene 456(1-2): 1-14.

22. Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the myosin light chains 1,2 and 3 in the muscle of blackspot seabream, Pagellus bogaraveo, during development. Fish Physiology and Biochemistry 36: 1125-1132.


1. Power, DM, Guerreiro, PMG, Hubbard, PC, Huertas, M, Morgado, IM, Fuentes, J. 2010. Endocrinology applied to aquaculture finfish. In: Koumondouros, G (Ed). Recent Advances in Aquaculture Research, Transworld Research Network, Kerala, pp. 1-54.

2. Brown, CL, Power, DM, Núñez, JM. 2010. Disorders of development in fish. In: Fish Diseases and Disorders, Vol 2, 2nd edition, CABI, Oxon, UK.

3. Ozorio, ROA, Hubbard, PC, Barata, EN, Valente, LMP, Canário, AVM. 2010. Olfactory sensitivity to amino acids in the blackspot seabream (Pagellus Bogaraveo, Brünnich 1768): How effective is


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the electro-olfactogram in seawater?. Aquaculture Europe 2010, 5-10 October, Porto, Portugal. European Aquaculture Society, pp. 946-947.

4. Louro, B, Hadjipavlou, G, Hemani, G, Leach, R, Nadaf, J, Rowe, S, de Koning, DJ. 2009. Extensive QTL and Association Analyses of the QTLMAS2009 Data. 13th QTL-MAS Workshop, BMC Proceedings 4 (Suppl 1): S11.


1. Guerreiro, PM, Bataille, AM, Renfro, JL. 2010. PiT-like transporters are associated with inorganic phosphate transport by choroid plexus of spiny dogfish shark (Squalus acanthias). Bulletin MDIBL 49: p.5.


MASTER THESES

Silva, Maria João. 2010. Control of ammonia during fish transport: the usage of AmQuel® in laboratory trials. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Teresa Modesto, Co-supervisor: Pedro Guerreiro.

Filipe, Alexandra. 2010. Clonagem e análise de sequências codificantes de terpeno sintases das plantas aromáticas Thymus mastichina e Thymus albicans. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Natália Marques, Co-supervisor: João Cardoso.

Encarnação, Sandra Paula. 2010. Caracterização dos mecanismos de transporte epitelial no intestino da Dourada, Sparus auratus. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Juan Fuentes, Co-supervisor: Deborah Power.

Elias, Carlos Alexandre. 2010. Produção e expressão de péptidos recombinantes contra dsRNA. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Fernando Cardoso (INETI/UTPAM), Co-supervisor: Deborah Power.

Sobral, João Miguel Banha. 2010. Influência do gene HMOX1 (Heme Oxigenase I) na genética dos acidentes vasculares cerebrais. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Astrid Vicente (INS Dr Ricardo Jorge), Co-supervisor: Deborah Power.

Andrade, André Alçada Baptista Rebelo. 2010. Identification of reliable reference gene(s) for quantitative-PCR studies in male and female tissues and during oogenesis in the Mozambique tilapia, Oreochromis mossambicus. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Adelino Canario, Co-supervisor: Laurence Deloffre.


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PhD THESES

Martins, Florbela Alexandra Silva Vieira. 2010. Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power.

Guerreiro, Liliana Isabel Tomé dos Anjos. 2010. Biochemical and biological characteristics of novel piscine forms of parathyroid hormone and parathyroid hormone related peptide: a proteomic approach. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power.



- 2nd UTEN Workshop 2010 – Marine and Bio-Sciences, Research Collaboration & Network Building for Commercialization, CCMAR, University of Algarve, 27-28 September 2010, Faro, Portugal. Canario AVM – Organizer. http://utenportugal.org/events/marine-science-workshop/

- 5th and 6th CCMAR Seminar Sessions (2009/2010 – 2010/2011) Weekly seminars (October through June) featuring in-house and external invited speakers. These seminars are meant to inform CCMAR members on what research is being done in the various groups, introduce new members and collaborators or invite scientists or entities with relevant expertise or information. These sessions provide a forum for discussion and collaboration. Guerreiro PM – Organizer.



In 2010 the group published with colleagues from 12 countries and 21 universities and research institutes from Europe and the USA. LIFECYCLE is an FP7 Project with 14 partners (http://www.lifecycle-fp7.eu/); Sharebiotech is a Atlantic Area biotechnology project of 10 partners, half foreign. The group leader coordinates participation infrastructure projects ASSEMBLE (http://assemblemarine.org) ESFRI infrastructure European Marine Biological Resource Centre (http://www.embrc.eu/). International projects submitted for funding (MG4U, Euromarine). Representative in COST Action 867 “Welfare of Fish in European Aquaculture” (P Guerreiro). Organized international Workshop Marine and Bio-Sciences Research Collaboration & Network Building for Commercialization with US, Portuguese and German institutions. Oral communications at international conferences Aquaculture Europe 2010, Porto, Portugal; 13th QTL-MAS Workshop, Wageningen, The Netherlands; Plant and Animal Genomics Conference XVIII, January 8-14, San Diego USA; 32nd Conference for the International Society for Animal Genetics, Edinburgh, UK; 9th International Congress on the Biology of Fish, Barcelona, Spain. Scientists from Spain (Univ Barcelona, Univ Cadiz), Sweden

http://utenportugal.org/events/marine-science-workshop/

http://www.lifecycle-fp7.eu/


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(Univ Goteborg), UK (British Antarctic Survey), Denmark (Univ Copenhagen), Greece (Univ Thessaly), Germany (U Dusseldorf) made work visits to our laboratory (1 week – 5 months). Researchers from our group made research visits to Denmark (Univ Copenhagen), Sweden (Univ Goteborg), USA ( Mount Desert Island Biological Laboratory), UK (British Antarctic Survey, Univ Aberdeen), Spain (Univ Cadiz) for periods of 1 week to 3 months. D Power is associate editor of General Comparative Endocrinology (Elsevier); A Canario is Advisory editor of Acta Ethologica (Springer) and Associate Editor for Animal Endocrinology of Endocrinology Studies (Pagepress). Group members reviewed ca. 50 manuscripts for international journals. Reviewed project applications for the European Commission and programmes in several countries (Argentina, France, Spain, UK, The Netherlands, USA, Canada). A Canario coordinates and is one of the mentors of the doctoral programme ERASMUS Mundus MARES (www.mares-eu.org). D Power is vice-presidente of Iberian Association of Comparative Endocrinology.


http://www.mares-eu.org/


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Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)



Ecology and Restoration of Estuarine and Coastal Habitats (ECOREACH)


Luis Manuel Zambujal Chicharo






- National funding

13.894 EUR, FCT, PTDC/MAR/098643/2008, MODELA - Modelling the dynamics of fish larvae and relevant oceanographic processes.

37.197 EUR, FCT, ERA-NET CIRCLE-MED, ERA-CIRCLE/0004/2007, ACID BIV - The integrated impacts of marine acidification, temperature and precipitation changes on coastal biodiversity and fisheries: how to adapt?.


1. Objectives

Our research are mainly focused on Biological and physical-chemical processes in transitional water (from catchment to marine ecosystems) namely, primary and secondary production processes, nutrient cycles, the characterization of habitats, population and community dynamics and trophic interactions. We aimed to understand the natural variability of aquatic ecosystems (inflow, tides, upwelling, winds) and the ecologic impacts by human activities (fisheries, pollution, dams, NIS/alien species, etc), particularly at rivers, estuarine and coastal areas. The study of nursery functions of coastal ecosystems is also an objective of our group, especially focused on the larval condition and growth. Considering the current scenario for climatic changes another aim of our group is to analyze the effect of acidification in marine invertebrate.


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The main achievement in our group was related to the project CircleMed: The integrated impacts of marine acidification, temperature and precipitation changes on bivalve coastal biodiversity and fisheries: how to adapt? (ACIDBIV). Results obtained in the Venice lagoon highlighted effects of seawater acidification at biochemical, cellular and organism level in both species considered. Detrimental effects were observed in adults exposed for short time to reduced pH and temperature/salinity variations (as foreseen in climate change scenarios), as well as the longterm exposure of juvenile bivalves to reduced pH, which caused mortality and severe damage to the shells. In general, the experiments done in the Ria Formosa lagoon showed a less detrimental response of the studied bivalves to seawater acidification. Juveniles of both species studied were able to survive more than 80 days exposed to extreme reductions of seawater pH (0.7 units). No dissolution or damage to the shells was observed, although mussels in the acidified treatments showed reduced net calcification, indicating that the composition or structure of the shell was affected. Some physiological parameters (RNA:DNA ratio, ingestion rates, excretion rates and respiration rates) of R. decussatus were significantly affected by the acidification conditions tested. The effects of seawater acidification on the different physiological rates of bivalves studied were consistently stronger for the extreme pH reduction (-0.7 pH units). The responses of the common species (M. galloprovincialis) also differed between locations and according to the stage of life cycle. This suggest that, even for the same species or process, the responses may be variable at local scales, which emphasizes the danger of extrapolating results from a few model species or from one region to another.

8d. Productivity


1. Morais, P, Babaluk, J, Correia, AT, Chícharo, MA, Campbell, JL, Chícharo, L. 2010. Diversity of anchovy migration patterns in a European temperate estuary and in its adjacent coastal area: Implications for fishery management. Journal Sea Research, 64: 295-303. IF=1.556.

2. Leitão, A, Carvalho, S, Ben-Hamadou, R, Gaspar, MB. 2010. Cytogenetics of Hediste diversicolor (Annelida: Polychaeta) and comparative karyological analysis within Nereididae. Aquatic Biology 10: 193-200.

In press

3. Morais, P, Martins, F, Chícharo, MA, Lopes, J, Chícharo, L. (in press). Merging anchovy eggs abundance into a hydrodynamic model as an assessment tool for estuarine ecohydrological management. River Research Applications, doi: 10.1002/rra.1443.


1. Ben‐Hamadou, R, Chícharo, MA, Cruz, J, Madeira, C, Morais, P, Chícharo, L. 2010. Spatial distribution of zooplankton communities in the Algarve coastal zone (Northeast Atlantic Ocean) In: Gislason, A, Gorsky, G. (Eds). Proceedings of the Joint ICES/CIESM Workshop to Compare


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Zooplankton Ecology and Methodologies between the Mediterranean and the North Atlantic (WKZEM). ICES Cooperative Research Report No. 300. 2-7.

2. Chícharo, L, Ben-Hamadou, R, Amaral, A, Range, P, Mateus, C, Piló, D, Marques, R, Morais, P, Chícharo, MA. 2010. Applying the Ecohydrology approach for the sustainable functioning of coastal ecosystems. In: Hydrocomplexity: New Tools for Solving Wicked Water Problems. IAHS Publ. 338. pp. 163-165.


1. Chícharo, L, Chícharo, MA, Ben-Hamadou, R. 2010. Eco-hidrologia em estuários e zonas costeiras: o caso do Estuário de Guadiana. In: A Gestão da água e dos Recursos Hídricos. Revista Internacional em Língua Portuguesa, Vol III, nº22: 55-66.


PhD THESES

Faria, Ana. 2010. Ontogeny of behavioural abilities in temperate reef fish larvae. PhD thesis, University of Algarve. Supervisors: Emanuel Gonçalves (ISPA), Maria Alexandra Chícharo.





- Research

ACID BIV, ERA-CIRCLE/0004/2007), The integrated impacts of marine acidification, temperature and precipitation changes on bivalve coastal biodiversity and fisheries: how to adapt? CIRCLE-MED (Portugal, Spain, Italy, Tunisie), April 2008 - March 2011. Partners University of Padova, CSIC Vigo, University Bizerte.

Bilateral FCT-MESRST project (Portugal-Tunisia), GUAMED - Coastal ecology and global changes. A comparative study between Guadiana and Medjerda coastal zones, 2008-2010.

- Graduate Training

Advanced Course on Tools to the ecohydrologic management of coastal systems in arid and semi arid zones, 17-21 July 2010, Cairo, Egypt.

- Collaborative publication


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Morais, P, Babaluk, J, Correia, AT, Chícharo, MA, Campbell, JL, Chícharo, L. 2010. Diversity of anchovy migration patterns in a European temperate estuary and in its adjacent coastal area: Implications for fishery management. Journal Sea Research 64: 295-303. IF(2009)=1.556, IF(2005-2009)=1.828.

Faria, AM, Muha, T, Morote, E, Chícharo, MA. 2011. Influence of starvation on the critical swimming behaviour of the Senegalese sole (Solea senegalensis) and its relationship with RNA/DNA ratios during ontogeny. Scientia Marina 75(1): 87-94. IF(2005-2009)=1.515.



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Ecophysiology



Ecophysiology


João José Oliveira Dias Coimbra


CIIMAR- Centro Interdisciplinar de Investigação Marinha e Ambiental, Porto


In 2010, the main sources of funding for the group came from FCT project included 98.000 EUR as principal investigators and ca. 17.000 EUR as members of research teams, as well as FCT pluriannual funding (38.500 EUR). In total amounting to for approximately 153.500 EUR. Other sources of funding included EU COST action TD0903 (150.000 EUR), SFI (Sea Fisheries Institute in Gdynia, Poland) (200 EUR) and collaboration on a UK BBSRC grant (2.000 EUR).


1. Objectives

10. Investigate the physiological challenges faced by migratory fishes (Anguilla anguilla, Platichthys flesus, Petromyzon marinus, Scyliorhinus cannicula, Pagellus bogaraveo) and other aquatic organisms, focusing on the abiotic factors of salinity and hydrostatic pressure. The species that are investigated are of commercial and/or biological significance.

11. Determine migratory patterns of fishes (A. anguilla, Conger conger, P. flesus) at different life history stages using otolith microstructure and microchemistry techniques. The use of otolith elemental and isotopic signatures is being use to study stock and population structure for several Portuguese important fishing species, such as C. conger, S. acantharus, D. vulgaris and T. luscus.

12. The potential use of the chronological proprieties of fish otoliths is being tested in an attempt to use otoliths biomarkers of aquatic metallic pollution.

13. Defining the mechanisms for dealing with physiological constraints and challenges of ammonotelism in fishes.


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14. Elucidate the mechanisms of shell calcification and biomineralization in freshwater bivalves (Anodonta cygnea) under normal and ecotoxic conditions. In addition, to evaluate the immune responses to toxic and pathogenic agents in bivalves and to define the culture conditions for freshwater bivalves (A. cygnea, A. anatina, Potomida littoralis, Unio delphinus and Margaritifera margaritifera).

15. Technological advances to aid in biological discovers and monitoring of the environment. Specifically the development of methods for the non-invasive monitoring of physiological parameters and the development of hyperbaric systems for the maintenance of fish and other aquatic organisms for investigating hydrostatic pressure effects on physiology.

2. Main Achievements

- Completion of microarray experiments in both eel (Anguilla anguilla) and trout (Oncorhynchus mykiss in response to increased hydrostatic pressure to identify an number of pressure sensitive genes and zebrafish (Danio rerio) to ammonia exposure to identify ammonia responsive genes.

- Determination of a comprehensive battery of biomarkers in several fish species in reaction to high hydrostatic pressure. In 2010, experiments were conducted with dogfish (Scyliorhinus canicula), rainbow trout (O. mykiss) and turbot (Psetta maxima).

- Elucidation of the stock structure in the Portuguese north coast for T. luscus. Population structure of two sparids fishes (D. vulgaris and S. cantharus) in the SW Portuguese coast.

- Design of the new method for studying the effects of bivalve fluids on the mineralization of biosynthetic membranes under specific conditions

- Discovery and assessments of new population of freshwater bivalve Margaritifera margaritifera in the Tamega basin (north of Portugal)

- Identification of the branchial mechanism of ammonia elimination in the air-breathing fish Misgurnus anguilliacaudatus as involving coupling of a rhesus ammonia transporter and proton pump.

- Inclusion in EU level networks (e.g. COST ACTION - TD0903) and participation in national (FCT) and international (UK BBSRC, Canada NSERC) collaborative projects.

- Dissemination of results generated by the group. In 2010 we collectively have 16 international peer reviewed publications in addition to two other publications. We also have 19 conference presentations, 9 of which were paper presentations. Group members have also been actively involved in education of the public.

- Research training at the undergraduate and graduate levels (3 MSc completed in 2010).


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8d. Productivity


1. Antunes, F, Hinzmann, M, Lopes-Lima, M, Machado, J, da Costa, PM. 2010. Association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of Anodonta cygnea (Linnaeus, 1758). Microbial Ecology 60: 304-309.

2. Campos, J, Bio, A, Cardoso, JFMF, Dapper, R, Witte, JIJ, Van der Veer, HW. 2010. Fluctuations of brown shrimp Crangon crangon abundance in the western Dutch Wadden Sea. Marine Ecolology Progress Series 405: 203-219.

3. Chew, SF, Tng, YY, Wee, NL, Tok, CY, Wilson, JM, Ip, YK. 2010. Intestinal osmoregulatory acclimation and nitrogen metabolism in juveniles of the freshwater marble goby exposed to seawater. Journal of Comparative Physiology B 180: 511-520.

4. Costa-Dias, S, Dias, E, Lobon-Cervia, J, Antunes, C, Coimbra J. 2010. Infection by Anguillicoloides crassus in a riverine stock of European eel, Anguilla anguilla. Fisheries Management and Ecology 17: 485-492.

5. Costa-Dias, S, Freitas, V, Sousa, R, Antunes, C. 2010. Factors influencing epibenthic assemblages in the Minho estuary (NW Iberian Peninsula). Marine Pollution Bulletin 61: 240-246.

6. Costa-Dias, S, Sousa, R, Antunes, C. 2010. Ecological quality assessment of the lower Lima estuary. Marine Pollution Bulletin 61: 234-239.

7. Freitas, V, Cardoso, JFMF, Lika, K, Peck, MA, Campos, J, Kooijman, SALM, Van der Veer, HW. 2010. Temperature tolerance and energetics: a dynamic energy budget-based comparison of North Atlantic marine species. Philosophical Transactions of the Royal Society B 365: 3553-3565.

8. Fu, C, Wilson, JM, Rombough, P, Brauner, CJ. 2010. Ions first: Naþ uptake shifts from the skin to the gills before O2 uptake in developing rainbow trout, Oncorhynchus mykiss. Proceedings of the Royal Society B 277: 1553-1560.

9. Kopecka-Pilarczyk, J. 2010. Effect of exposure to polycyclic aromatic hydrocarbons on Na+/K+-ATPase in juvenile gilthead seabream Sparus aurata. Short communication. Journal of Fish Biology 76(3): 716-722.

10. Kopecka-Pilarczyk, J. 2010. In vitro effects of pesticides and metals on the activity of acetylcholinesterase (AChE) from different tissues of the blue mussel, Mytilus trossulus L. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes 45(1): 46-52.

11. Kopecka-Pilarczyk, J. 2010. The effect of pesticides and metals on acetylcholinesterase (AChE) in various tissues of blue mussel (Mytilus trossulus L.) in short-term in vivo exposures at different temperatures. Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes 45(4): 336-346.


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12. Kopecka-Pilarczyk, J, Coimbra, J. 2010. Short term high hydrostatic pressure effect on selected biomarkers in silver eel (Anguilla anguilla). Journal of Experimental Marine Biology and Ecology 391: 118-124.

13. Kopecka-Pilarczyk, J, Coimbra, J. 2010. The effect of elevated hydrostatic pressure upon selected biomarkers in juvenile blackspot seabream Pagellus bogaraveo in a 14 day-long experiment. Brief communication. Journal of Fish Biology 77: 279-284.

14. Lopes, A, Lopes-Lima, M, Bobos, I, Ferreira, J, Gomes, S, Reis, R, Mano, J, Machado, J. 2010. The effects of Anodonta cygnea biological fluids on biomineralization of chitosan membranes. Journal of Membrane Science 364: 82-89.

15. Lopes-Lima, M, Rocha, A, Goncalves, F, Andrade, J, Machado, J. 2010. Microstructural characterization of inner shell layers in the freshwater bivalve Anodonta cygnea. Journal of Shellfish Research 29: 969-973.

16. McGuire, A, Aluru, N, Takemura, A, Weil, R, Wilson, JM, Vijayan, MM. 2010. Osmotic shock adaptation by cortisol involves the suppression of gill metabolic capacity in Mozambique Tilapia. General and Comparative Endocrinology 165: 321-329.

17. Morais, P, Babaluk, J, Correia, AT, Chícharo, MA, Campbell, JL, Chícharo, L. 2010. Diversity of anchovy migration patterns in an European temperate estuary and in its adjacent coastal area: Implications for fishery management. Journal of Sea Research 64: 295-303.

In press

18. Machado, J, Lopes-Lima, M. (in press). Calcification mechanism in freshwater mussels: potential targets for cadmium. Toxicological & Environmental Chemistry, doi: 10.1080/02772248.2010.503656

19. Moreira-Silva, JC, Tsui, TKN, Coimbra, JC, Ip, YK, Wilson, JM. (in press). Branchial ammonia excretion in the Asian weatherloach Misgurnus anguillicaudatus (Teleostei: Cobitidae) Comparative Biochemistry and Physiology C, doi: 10.1016/j.cbpc.2009.08.006.


1. Wilson, JM, Castro, LFC. 2010. Morphological diversity of the gastrointestinal tract in fishes. In: Grosell, M, Farrell, AP, Brauner, CJ (Eds). The multifunctional gut of fish – Volume 30, Elsevier Inc., pp. 1-55. ISBN: 978-0-12-374982-6.

2. Cardoso, JFMF, Freitas, SM, Veer, HW, Machado, JP. 2010. Age determination in bivalves: validation of the seasonality in growth bands – a first test for the soft-shell clam Mya arenaria. Proceedings of the workshop Bivalves biomineralisation: Archival potential and proxy incorporation, The Royal Flemish Academy of Belgium for Science and the Arts, Belgium, KVAB D/2010/0455/00: 63-66.



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MASTER THESES

Capela, Ricardo. 2010. Fish otoliths as potential chronological biomarkers of environmental aquatic contamination by heavy metals. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: Alberto Correia, Co-supervisor: Bruno Nunes (University Fernando Pessoa, CESAM).

Gonçalves, Annie. 2010. Ecotoxicological and histological assessment of the effects of the benzalconium chloride in the mosquito fish (Gambusia holbrooki) after an acute exposition. Master in Pharmaceutical Sciences, Health Sciences Faculty, University Fernando Pessoa. Supervisor: Bruno Nunes (University Fernado Pessoa, CESAM), Co-supervisor: Alberto Correia.

Silva, Diogo. 2010. Discrimination of Trisopterus luscus (Linnaeus 1758) stocks in the northern of Portugal using otolith elemental fingerprints. Master thesis in Sea Sciences - Marine Resources, ICBAS, University of Porto. Supervisor: Alberto Correia, Co-supervisor: Paulo Santos.



- I Jornadas de Medicina Integrativa, Centro de Formação DEFI do Centro Hospitalar do Porto (HGSA), 26-29 November 2010, Porto, Portugal. J Machado – Member of the Organizing Commitee.

- Luso-Brasileiro de Medicinas Naturais nas suas duas vertentes: Reabilitação Motora e Fitoterapia Chinesa, 29 September 2010, Porto, Portugal. J Machado – Member of Honour Committee and Moderator.


8. Internationalization (Collaborative publication, Research, Graduate Training Networks or other forms of participation of the Research Group at the international level)

In 2010, the Ecophysiology group had 10 publications and 9 conference presentations with international collaborators. In addition through collaborations on, we have been involved in the advanced training of doctoral researchers in Porto (Luciana Rodrigues de Souza Bastos, in collaboration with Prof C Arruda de Oliveira Freire, Federal University of Paraná, Brasil; Koorosh Sarvi Moghanloo, in collaboration with M Kalbassi, Tarbiat Modares University (TMU), Iran). J Wilson in the group is also involved in collaborative research with Prof MP Wilkie, Wilfred Laurier University, funded by the Natural Sciences and Research Council, Canada; and RW Wilson, University of Exeter, funded by the British Biological Sciences Research Council, BBSRC, UK. J Machado has active collaborations to study bivalve shell structure with Prof A Checa,


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University of Granada, Spain; Prof H Frank, University of Bayreuth, Germany; T Vanderlei Alves, CRPNM, Instituto Federal de Educação, Ciência e Tecnologia da Paraíba - IFPB, Brazil.

A Correia is involved in collaborative research with Paul Hamer, Victoria Dept Industries, Australia, and Prof. Alcides Sial, Federal Univ Pernambuco, Brazil, concerning the use of otolith isotopes and elemental signatures as a tool for fish stock discrimination purposes.

We also currently have a post-doctoral researcher, A Damasceno-Oliveira, with a cross appointment to the Liverpool Microarray Facility and Centre for BioArray Innovation, University of Liverpool with Prof A Cossins. Post-doctoral researcher, J Kopecka-Pilarczyk has established a collaboration with H Dabrowska from Sea Fisheries Institute in Gdynia, Poland, on comparison of organic contaminant levels and selected biomarkers in flounder from the Douro and Vistula River estuaries. In addition JFMF Cardoso is completing a joint post doc in the Department of Ecology of the Royal Netherlands Institute for Sea Research on bivalve research. Through provisions in their FCT fellowships or project funding, students have worked abroad (O Gonçalves, MI Pascoa, U Waterloo).

9. Government/organization contract research (Work carried out by the Research Group that resulted in a publication or report. Of particular importance are those involved in public policy advice)

Ecophysiology Group participates in NATURA-MINHO-MIÑO, a project funded by Interreg IV POCTEP, the Transboundary territorial cooperation program between North of Portugal and Galicia. This project aims the join valorization of natural resources in the river Minho Hydrographical basin, the promotion of sustainable development and biodiversity and habitat conservation (Natura 2000) in the transboundary territory of North of Portugal and Galicia. It involves the participation of regional authorities (General Directorate of Nature Conservation in Galicia and ICBN - Institute for Nature Conservation and Biodiversity of the Portuguese Ministry for the Environment) assuring the effective involvement in public policy advice.


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Ecotoxicology and Ecology (ECOTOX)



Ecotoxicology and Ecology (ECOTOX)


Lúcia Maria das Candeias Guilhermino




In 2010, ECOTOX brought directly to CIIMAR 901.452 EUR and other funds through projects coordinated by other CIIMAR members; 855.960 EUR came directly to the group: FCT and other research projects (772.460 EUR), pluriannual FCT funds (28.000 EUR), students fees (17.500 EUR) and other (38.000 EUR). In addition, ECOTOX members participated in international meetings with travelling/stay expenses directly covered and several projects are under evaluation (not included in the budget).

Detailed:

- 28.000 EUR FCT pluriannual funding


199.776 EUR (new project), PTDC/AAC-AMB/110331/2009, 2011-2014. PI: Lúcia Guilhermino.

50.040 EUR, LTER/BIA-BEC/0019/2009, 2011-2014. Responsible at CIIMAR: Lúcia Guilhermino.

119.068 EUR, PTDC/MAR/108369/2008, 2010-2014. PI: Natividade Vieira.

189.814 EUR, PTDC/MAR/71143/2006, 2008-2011. PI : Laura Guimarães.

57.987 EUR, EU ERA-AMPERA 0001/2007, 2008-2011 + BI grant. PI: Lúcia Guilhermino.

38.000 EUR, University, industry, other sources. (Lúcia Guilhermino)

2.500 EUR other projects.

17.500 EUR PhD student fees.


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1. Objectives

The central objective of ECOTOX Lab is to investigate the evolution and functioning of aquatic ecosystems under anthropogenic and “natural” stress, with emphasis on estuarine and coastal ecosystems. We are particularly interested in the assessment of long-term alterations induced by global stressors (e.g. pollution, biological invasions and climate changes) on both biotic and abiotic components of ecosystems, in understanding how and why these alterations may affect their structure and functioning and how to achieve a compromise between biodiversity conservation and sustainable development in global change scenarios. For this, we have been working in several Portuguese ecosystems and in tropical selected areas, using 3 complementary approaches: comparison of similar ecosystems under different types and/or levels of pollution (and other global stressors), study over time of the same ecosystem and comparison of similar problems in temperate and tropical regions. In all cases, integrated strategies including methods from ecotoxicology, ecology, genetics, and chemistry have been used.

Specifically we intend to:

- Identify and quantify ecosystems services in the Portuguese coast and selected areas in the tropics, identify and characterize their problems (e.g. chemical pollution, eutrophication, landscape alterations, climate changes, biological invasions), study their evolution over time, develop novel methods and approaches for ecological risk assessment, perform ecological and ecotoxicological long-term monitoring of selected areas and ecological risk assessment .

- Assess the impact of stressors on organisms, populations and on the whole ecosystem by using conventional approaches such as ecological and ecotoxicological monitoring, bioaccumulation and biomagnification studies, ecological risk assessment and new methods developed by the group (e.g. in situ and laboratory bioassays with native species, environmental biomarkers).

- Understand the mechanisms of action of stressors at sub-individual, individual and population levels and how the presence of stressors can influence species relationships (e.g. predation, competition, facilitation), biotic/abiotic interactions, energy transfer and nutrient cycling in ecosystems.

- Contribute for the achievement of a compromise between biodiversity conservation and sustainability by providing tools, results and recommendations to decision-makers supporting scientific-based environmental policies and ecosystem management.


- 23 full papers published in international scientific per-reviewed journals.

- 2 books and 1 book chapter.


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- Effects of PAHs, pesticides, metals and pharmaceuticals on several marine and freshwater species of temperate and tropical areas, including mechanisms of toxicity and biotransformation.

- Relationships between several biomarkers and behaviour parameters.

- Knowledge about contamination levels in NW Portuguese coast (Aveiro lagoon – Spanish border), Minho, Lima, Cávado and Douro Rivers estuaries and Aveiro lagoon, using integrated approaches including water quality variables, chemical analysis (e.g. metals, PAHs, pesticides), bioaccumulation factors, biomarkers and condition indexes in fish (eels; common goby).

- Knowledge on the freshwater tidal area of Minho estuary, mainly of the macrobenthic community, population dynamics of key-species, impact of 2009 heat wave on the community and populations resistance and resilience.

- Knowledge on the biological effects of contaminants in coastal and estuarine fish.

- Identification and characterization of the non-native invasive species introduced in the Minho and Lima rivers. Particularly attention was focused in the non-native invasive species Corbicula fluminea, including its impact on native species and on ecosystems

- 1 PhD Thesis and 6 MSc Thesis concluded.

- Advanced training in Ecotoxicology and Ecology of 10 students, including 5 from other countries.

- 8 Visits of senior researchers from Brazil, Finland, Italy, Mexico, Norway, The Netherlands and Spain.

8d. Productivity


1. Afonso, MJ, Chaminé, H, Marques, JM, Carreira, PM, Guimarães, L, Guilhermino, L, Gomes, A, Fonseca, PE, Pires, A, Rocha, F. 2010. Environmental issues in urban groundwater systems: a multidisciplinary study of the Paranhos and Salgueiros spring waters, Porto (NW Portugal). Environmental Earth Sciences 61: 379-392.

2. Agra, A, Guilhermino, L, Soares, A, Barata, C. 2010. Genetic costs of tolerance to metals in Daphnia longispina populations historically exposed to a copper mine drainage. Environmental Toxicology and Chemistry 29: 939-946.

3. Almeida, JR, Oliveira, C, Gravato, C, Guilhermino, L. 2010. Linking behavioural alterations with biomarkers responses in the European seabass Dicentrarchus labrax L. exposed to the organophosphate pesticide fenitrothion. Ecotoxicology 19: 1369-1381.


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4. Costa-Dias, S, Sousa, R, Antunes, C. 2010. Ecological quality assessment of the lower Lima estuary. Marine Pollution Bulletin 61: 234-239.

5. Costa-Dias, S, Freitas, V, Sousa, R, Antunes, C. 2010. Factors influencing epibenthic assemblages in the Minho estuary (NW Iberian Peninsula). Marine Pollution Bulletin 61: 240-246.

6. Ferreira, F, Santos, MM, Reis-Henriques, MA, Vieira, NM, Monteiro, NM. 2010. Sexing blennies using genital papilla morphology or anogenital distance. Journal of Fish Biology 77: 1432-1438.

7. Frasco, MF, Erzen, I, Stojan, J, Guilhermino, L. 2010. Localization and properties of cholinesterases in the common prawn (Palaemon serratus): a kinetic-histochemical study. Biological Bulletin 218: 1-5.

8. Gravato, C, Guimarães, L, Santos, J, Faria, M, Alves, A, Guilhermino, L. 2010. Comparative study about the effects of pollution on glass and yellow eels (Anguilla anguilla) from the estuaries of Minho, Lima and Douro Rivers (NW Portugal). Ecotoxicology and Environmental Safety 73: 524-533.

9. Martínez-López, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, P, Guilhermino, L, García-Fernández, AJ. 2010. Blood delta-ALAD, lead and cadmium concentrations in spur-thighed tortoises (Testudo graeca) from Southeastern Spain and Northern Africa. Ecotoxicology 19: 670-677.

10. Menezes, S, Soares, AMVM, Guilhermino, L, Peck, MR. 2010. Can the activities of acetylcholinesterase and glutathione S-transferases of Crangon crangon (L.) be used as biomarkers of fuel oil exposure? Water, Air and Soil Pollution 208: 317-322.

11. Moreira, SM, Moreira-Santos, M, Rendón-von Osten, J, da silva, EM, Ribeiro, R, Guilhermino, L, Soares, AM. 2010. Ecotoxicological tools for the tropics: Sublethal assays with fish to evaluate edge-of-field pesticide runoff toxicity. Ecotoxicology and Environmental Safety 73: 893-899.

12. Oliva, M, de Canales, MLG, Gravato, C, Guilhermino, L, Perales, JA. 2010. Biochemical effects and polycyclic aromatic hydrocarbons (PAHs) in senegal sole (Solea senegalensis) from a Huelva estuary (SW Spain). Ecotoxicology and Environmental Safety 73: 1842-1851.

13. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2010. Reversing sex-role reversal competes only when you must. Behavioural Ecology Animal 79(4): 885-893.

14. Silva K, Vieira, MN, Almada, VC, Monteiro, NM. 2010. Female reproductive tactics: Scanning for male quality and number. Behavioural Ecology 20: 768-772

15. Wenning, RJ, Nunns Jr, WR, Stahl Jr, RG, Guilhermino, L, Menzie, C, Römbke, J, Helm, RC. 2010. Global climate change and environmental contaminants: a SETAC call for research. Integrated Environmental Assessment and Management 6(2): 197-198.


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In press

16. Amorim, MJB, Oliveira, E, Teixeira, AS, Gravato, CS, Loureiro, S, Guilhermino, L, Van Gestel, CAM, Soares, AMVM. (in press). Toxicity and bioaccumulation of phenanthrene in Enchytraeus albinus (Oligochaeta: Enchytraeidae). Environmental Toxicology and Chemistry 30.

17. Ferreira, F, Santos, MM, Reis-Henriques, MA, Vieira, N, Monteiro, NM. (in press). Sexing the shanny, Lipophrys pholis, using genital papilla morphology or anogenital distance. Notes on two other sympatric rocky intertidal blennies. Journal of Fish Biology.

18. Gomes, SIL, Novais, SC, Gravato, C, Guilhermino, L, Scott-Fordsmand, JJ, Soares, AMVM, Amorim, MJB. (in press). Effect of Cu-nanoparticles versus one Cu-salt: analysis of stress and neuro-muscular biomarkers response in Enchytraeus albidus (Oligochaeta). Nanotoxicology.

19. Howcroft, CF, Gravato, C, Amorim, MJ, Novais, SC, Soares, AM, Guilhermino, L. (in press). Biochemical characterization of cholinesterases in Enchytraeus albidus and assessment of in vivo and in vitro effects of different soil properties, copper and phenmedipham. Ecotoxicology.

20. Ilarri, M, Antunes, C, Guilhermino, L, Sousa, R. (in press). Massive mortality of the Asian clam Corbicula fluminea in a highly invaded area. Biological Invasions.

21. Rubal, M., Guilhermino, L., Medina, M.H. (in press). Two strategies to live in low chronic pollution estuaries: the potential role of life style. Ecotoxicology and Environmental Safety.

22. Sarria, MP, Santos, MM, Reis-Henriques, MA, Vieira, NM, Monteiro, NM. (in press). The unpredictable effects of mixtures of androgenic and estrogenic chemicals on fish early life. Environment International, doi:10.1016/j.envint.2010.11.004.

23. Vieira, MN, Bio, A. (in press). Functioning and zooplankton ecology of a Portuguese artisanal salina. Journal Sea Research.


1. Afonso, MJ, Pires, A, Chaminé, HI, Marques, JM, Guimarães, L, Guilhermino, L, Rocha, FT. 2010. Aquifer vulnerability assessment of urban areas using a GIS-based cartography: Paranhos groundwater pilot site, Porto, NW Portugal. In: Paliwal, BS (Ed). Global Groundwater Resources and Management. Scientific Publishers, Jodhpur, pp. 259-278.

2. Guilhermino, L, Guimarães, L. 2010. Are complex approaches a real advantage in the assessment of ecotoxicological effects in real scenarios? Comparative Biochemistry and Physiology A 157: S19-S20.

3. Guimarães, L, Gravato, C, Santos, J, Guilhermino, L. 2010. Impact of pollution on the development of glass eels (Anguilla anguilla) entering three NW Portuguese estuaries. Comparative Biochemistry and Physiology A - Molecular and Integrative Physiology 157: S25.


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4. Hernández-Garcia, A, Romero, D, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Effects on enzymatic antioxidant system of mallard (Anas platyrhynchos) erythrocytes exposed in vitro to lead and cadmium. Toxicology Letters 196: 128-129.

5. Mesquita, SR, Guilhermino, L, Guimarães, L. 2010. Assessing the effects of the selective serotonin reuptake inhibitor fluoxetine on Carcinus maenas using locomotor behaviour and biomarkers as effect criteria. Comparative Biochemistry and Physiology A 157: S23.

6. Hernández-Garcia, A, Romero, D, Tagliati, CA, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Glutathione S-transferase activity, glutathione and lipid peroxidation levels in mallard (Anas platyrhynchos) erythrocytes exposed to lead and cadmium in vitro. Toxicology Letters 196: 128.

7. Oliveira, M, Vieira, LR, Gravato, C, Guilhermino, L. 2010. Acute effects of pyrene on the common goby Pomatoschistus microps (Teleostei, Gobiidae). Toxicology Letters 196, 127-128.


1. Almeida, JR, Sá, N. 2010. Guia de Mergulho da Madeira (Madeira Diving Guide). Veraçor Editores, Ponta Delgada, 109p.

2. Fidalgo, ML, Sousa, L, Oliveira, A, Magalhães, P. 2010. Norte do sonho. Caminha e o estuário do rio Minho. Edição da Câmara Municipal de Caminha, Caminha, ISBN: 978-989-95920-4-9, 165p.


MASTER THESES

Oliveira, Cristiana, 2010. Effects of pesticides in biomarkers and behaviour of Palaemon serratus. Master thesis in Applied Biology: branch of Toxicology and Ecotoxicology, Department of Biology, University of Aveiro. Supervisor: Carlos Gravato.

Rodrigues, Carolina. 2010. Avaliação da Qualidade de Ecológica do Rio Ferreira nos Concelhos de Valongo e Paços de Ferreira. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

Sousa, Raquel Cristina Ferreira. 2010. Avaliação da Qualidade da água da Ribeira da Madalena. Influência da vegetação riparia nos grupos funcionais de macroinvertebrados bentónicos. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

Almeida, Helena Lopes. 2010. Avaliação da Qualidade da Biológica da água de um ecossistema fluvial - Ribeira da Madalena. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

Mesquita, Sofia. 2010. Testing the potential of chitobiase activity of Carcinus maenas as a biomarker in environmental studies. Master thesis in Contamination and Environmental


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Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: Laura Guimarães, Co-supervisor: Lúcia Guilhermino.

Duarte, Daniel João da Silva Tavares. 2010. Master thesis in Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: M Soto (University of the Basque Country), Co-supervisor: Lúcia Guilhermino.

PhD THESES

Lima, Inês de Marrazes. 2010. Ecotoxicological effects of petrochemical products on natural populations of Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Lúcia Guilhermino, Co-supervisor: Amadeu MVM Soares (CESAM, University of Aveiro).



- L Guilhermino is President of the Society of Environmental Toxicology and Chemistry – Europe (SETAC-Europe), chaired and organised several meetings and conference calls of the SETAC Europe Council, in cooperation with the SETAC Europe Executive Committee and the SETAC Europe office. One of the SETAC Europe Council Meetings (Chaired by L Guilhermino) and the Long Range Planning meeting (Chaired by the Vice-President of SETAC Europe – P Campbell) were held at CIIMAR. Porto, 27-28 September 2010.

- L Guilhermino was Chair of the Local Organising Committee, and of the Scientific Committee of the “12º Encontro Nacional de Ecologia – Serviços dos Ecossistemas. Desafios e ameaças num mundo em mudança”, organised by the Portuguese Society of Ecology, University of Porto (ICBAS & FCUP) and CIIMAR. Porto, 18-20 October 2010. She and several ECOTOX members were chairs of sessions on this meeting.

- L Guilhermino was member of the Scientific Committee, and organised and chaired (in cooperation with C Menzie, J Rombke, R Wenning) the Special Session “SS02 – Global stressors: climate changes, invasive species, and chemicals”. SETAC Europe 20th Annual Meeting – Science and Technology for Environmental Protection, Seville, 23- 27 May 2010.

- C Gravato was Chair of the session “Linking sub-individual effects with ecologically relevant parameters” during the SETAC Europe 20th Annual Meeting, Seville, 23- 27 May 2010.

- L Guilhermino was member of the Scientific Committee and Chair of the session “Ecotoxicology” of the XV Congress of the Iberian Association of Limnology, Ponta Delgada, 5- 9 July 2010.


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- L Guilhermino was Chair and Organizer (in cooperation with JC Marques and J Coimbra) of the session “Ecossistemas Marinhos”. Ciência 2010 – Encontro com a Ciência e Tecnologia em Portugal, Lisboa, 4-7 July 2010.

- L Guilhermino was Chair of one session in the topic “Ecological risk assessment in marine and estuarine ecosystems: how to integrate physicochemical, ecological and ecotoxicological methods” in the ECSA 47 Symposium. Figueira da Foz, 14-19 September 2010.

- Workshop PETGENES - Genetic Alterations in Aquatic Organisms After Exposure to Pollutants, CIIMAR & ICBAS, University of Porto. Organised by L Guilhermino, J Rotchell and L Guimarães, within the scope of a Bilateral Cooperation Programme between FCT and the British Council. CIIMAR, 12-13 January 2010.


The laboratory of Ecotoxicology and Ecology provides consulting and other services to industry, including toxicity tests and support for REACH, the European Community Regulation on chemicals and their safe use (EC 1907/2006). The laboratory has been also performing toxicity assays, environmental studies and consulting services regarding the management of residuals, sediments removal and other environmental matters for national industry, local and national Authorities (e.g. Municipalities). In addition, Lúcia Guilhermino has been participating in projects of the Autonomous University of Campeche, Mexico, in cooperation with oil and pesticide companies operating in the Gulf of Mexico to reduce the environmental and human health impact of the oil extraction and transformation, and of the use of pesticides in agriculture. Natividade Vieira collaborated with the laboratory of Semiarid Federal University of Rio Grande do Norte in the monitoring of solar saltern River Estuary Mossóro, and cooperated in studies of the river environment impact Potengi, with Petrobras and Biogeoquimica Laboratory, Federal University of Rio Grande do Norte, Brazil.


L Guilhermino is President of SETAC (Society of Environmental Toxicology and Chemistry) – Europe, Chair of its Executive Council, member of World Council of SETAC and of several Committees of this scientific society. She is also member (scientific expert) of the Working Group on Biological Effects of Environmental Contaminants of the ICES. R Sousa is a member of The European Group on Biological Invasions Council. L Guilhermino has been evaluator of projects/grants for the International Science Foundation. She and R Sousa have been evaluating projects for the Fondo Investigacion Científica de Argentina. L Guilhermino is Associate Editor of Biomarkers, member of the Editorial Board of Environmental Toxicology and Chemistry, and of Integrated Environmental Assessment and Management. R Sousa is member of the Editorial Board of Water SA. ECOTOX members with a PhD are regular reviewers of scientific SCI journals (e.g. Chemosphere, Environmental Pollution, Marine Ecology Progress Series, Environment International, Marine Pollution Bulletin, etc). The lab is a partner of the ERA-NET project RAMOCS (EU ERA-AMPERA 0001/2007), is involved in several networks and initiatives for international cooperation, and has intensive scientific cooperation with research groups in Europe, Africa, America and Asia, including: Belgium (Univ Antwerp), France (Univ Catholique Ouest), Norway (Univ Oslo, NIVA), Slovenia (Univ Ljublajana), Finland (Finish Environmental


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Institute), Spain (CSIC Torre La Sal, CSIC Barcelona, INIA, Univ Basque Country, Univ Coruna, Univ Extremadura, Univ Murcia, Univ Valencia, Univ Vigo); UK (Plymouth Marine Lab, Univ Sussex, Univ Cambridge), Brazil (Univ Federal Baía, Univ S Paulo and Univ, Federal do Rio Grande do Norte, Natal), Costa Rica (Univ Nacional), Mexico (Univ Campeche, CIAD Mazatlán, Univ Puebla), USA (Univ Georgia), Tunisia (Higher Inst Agronomy Choott Mariem), India (Univ Madras).

- Agra, A, Guilhermino, L, Soares, A, Barata, C. 2010. Environmental Toxicology and Chemistry 29: 939-946.

- Frasco, MF, Erzen, I, Stojan, J, Guilhermino, L. 2010. Biological Bulletin 218: 1-5.

- Martínez-López, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, P, Guilhermino, L, García-Fernández, AJ. 2010. Ecotoxicology 19: 670-677.

- Oliva, M, de Canales, MLG, Gravato, C, Guilhermino, L, Perales, JA. 2010. Ecotoxicology and Environmental Safety 73: 1842-1851.

- Wenning, RJ, Nunns Jr, WR, Stahl Jr, RG, Guilhermino, L, Menzie, C, Römbke, J, Helm, RC. 2010. Integrated Environmental Assessment and Management 6 (2): 197-198.


Several members of ECOTOX participated in meetings to establish priorities of scientific policy, science, research and action (e.g. management, sustainable development) at global, European, national and regional levels in several areas such as: Environment, Marine Sciences, Climate Changes, Chemicals use in Developing Countries. In addition, L Guilhermino is member of the Council of the North Hydrographic Region of Portugal (“Conselho da Região Hidrográfica do Norte”) assisting and advising the Administration of the Hydrographic Region of the North of Portugal (ARH-Norte) on the application of funds and adoption of strategies, plans and projects related with water use and management of river basins, among other matters, contributing for the development of the North Region of the country. She is member of the core group of the project LTER/BIA-BEC/0019/2009 (total budget: 199.932 EUR), recently funded by FCT and where the North and Centre Hydrographic Regions participate as end-use partners. She is also member of the Working Group on Biological Effects of Environmental Contaminants of the International Council for the Exploration of the Sea that develops methods and strategies to protect the marine environment assisting several international organizations and conventions such as OSPAR.


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Ecotoxicology, Genomics and Evolution (LEGE)



Ecotoxicology, Genomics and Evolution (LEGE)


Vitor Manuel Oliveira Vasconcelos





- National funding

140.818 EUR, FCT, PTDC/AMB/67075/2006. 2008-2010. PI: V Vasconcelos.

188.980 EUR, FCT, PTDC/MAR/68106/2006. 2008-2011. PI: M Santos, Researcher: A Antunes.

148.000 EUR, FCT, PTDC/BIA-BDE/70982/2006. 2008-2011. PI: A Antunes.

126.706 EUR, FCT, PTDC/MAR/099642/2008. 2010-2012. PI: V Vasconcelos.

87.024 EUR, FCT, PTDC/MAR/102258/2008. 2010-2012. PI: V Vasconcelos.

198.767 EUR, FCT, PTDC/AAC-AMB/102121/2008, NISTRACKS. 2010-2013. PI: L Guilhermino, Researcher: A Antunes.

199.997 EUR, FCT, PTDC/AAC-AMB/104983/2008. 2010-2013. Researcher: A Antunes.

124.570 EUR, FCT, PTDC/MAR/102638/2008. 2010-2012. Researcher: V Vasconcelos.

9.000 EUR, Pre-graduation projects, Univ Porto.

- European funding

138.033 EUR, INTERREG IV B Atlantic Area, ATLANTOX. 2009-2010. PI: V Vasconcelos.

195.514 EUR, INTERREG IV B Atlantic Area, PHARMATLANTIC. 2010-2012. PI: V Vasconcelos.

209.969 EUR, INTERREG IV B Atlantic Area, ShareBiotech. 2010-2012. PI: A Antunes.


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1. Objectives

LEGE- Laboratory of Ecotoxicology, Genomics and Evolution is a laboratory of CIIMAR and CIMAR-LA dedicated to the study of toxic organisms and its natural toxins diversity, including production mechanisms and control of their occurrence in a ecotoxicological and evolutionary genomic/proteomic perspective. It is also a major aim, to study the toxin-tolerance levels among varied organisms (from humans, to other animals and plants), which requires the molecular characterization of relevant cellular mechanisms, including detoxification, development and genetic disease onset.

Specific objectives:

- Ecotoxicological study of cyanobacteria and other potentially toxic organisms in marine, brackish and freshwaters. Occurrence of the main species and of the main toxins, and production of bioactive compounds with potential pharmacological interest.

- Ecotoxicological assays regarding the effects of neurotoxins and hepatotoxins in different organisms. Use of behavioral and histopathological alterations as endpoints. Studies on the bioaccumulation of toxins and research of possible toxin vectors. Human health risk assessment of toxin occurrence in drinking, recreational waters. Impact of contamination of toxins in water used for agriculture.

- Use of molecular and computational genomics/proteomics tools to characterize systemic functional behaviors of the cell and the organism (from humans, to other animals and plants). This includes understanding the interaction and adaptive evolution of cellular protein-coding genes operating in toxin production, detoxification, development, and genetic disease onset in various organisms.

- Use of molecular genomics for assessing the biogeography and phylogenetic relationships of toxic organisms (from cyanobacteria to metazoans) and non-toxic organisms with varied resistance to the effects of natural toxins.


The development of new analytical techniques, namely HPLC and Capilary Electrophoresis for the detection of BMMA in marine and freshwaters allowed its application to cyanobacteria from marine and estuarine environments. High levels of these neurotoxins were found in Portuguese isolates comparing with other published reports. All samples were positive for BMAA. BMAA presence was not related to the geographic origin or taxonomy of the isolates and no correlation between free and bound BMAA concentrations was observed within or between taxonomic groups. These data offer the first confirmation of the taxonomic and geographic ubiquity of BMAA from naturally-occurring populations of cyanobacteria in estuaries.

Allelopathy was studied related to compounds produced by cyanobacteria and we concluded that although some effects may be verified towards the germination and growth of several plants, cyanobacteria metabolites seem not to affect the dynamics of bacteria in Water Treatment Plants. Low densities of cyanobacteria have allelopathic effects in green algae and we


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isolated and structurally characterized new allelopathic substances – portoamides - produced by a freshwater Oscillatoria.

The marine toxin domoic acid was shown to induce toxic effects on sea bream. The non-existence of major permanent brain damage in S. aurata distinguishable under light microscopy, associated with reversibility of toxic signs suggests occurrence of neuronal recovery in affected individuals or higher individual DA resistance. Tetrodotoxin was found in some invertebrate species of the Portuguese Coast for the first time. The common toxins found in freshwater cyanobacteria were not detected in their marine and estuarine congeners, although toxic effects were observed against sea urchin embryos, virus and some human cell lines. The inhibition of HSV-1 and the strong cytotoxicity against L929 cells observed emphasizes the importance of evaluating the impact of those estuarine cyanobacteria on aquatic ecosystem and on human health.

Phylogenetic analyses assessed the evolution and functional diversification of the cytosolic GSTs, a large group of ubiquitous enzymes involved in detoxification and well known for their undesired side effects during chemotherapy. Protection against oxidative stress seems to be the major driver of positive selection in mammalian cGSTs, explaining the overall expansion pattern of this subfamily.

PhosphoProtein Phosphatases (PPP) enzymes involved in key cellular processes such as morphogenesis, synaptic transmission and apoptosis are the target of multiple naturally-occurring toxins, namely MC. The phylogeny of PPPs among metazoans and the molecular interaction of varied toxins with the PPPs, highlighted (1) which specific structural differences within the similarly folded catalytic core of the PPPs explain their diverse sensitivities to toxin inhibition, and (2) which structural features presented by the various toxins account for the differential inhibitory potency towards each PPP.

We assessed the phylogenetic relationships and adaptive evolution of important metazoan gene products involved in key cellular processes and often disrupted by environmental contaminants, which can be a cause of cancer and endocrine disruption. We characterized the gene/protein evolution of the Retinoid X Receptor (RXR), a nuclear receptor highly relevant the embryonic development of the vertebrate embryos; the matrix extracellular phosphoglycoprotein (MEPE) that belongs to a family of small integrin-binding ligand N-linked glycoproteins with an important role in skeleton development; and the Hedgehog (Hh) gene family that codifies a class of secreted proteins that act as signaling molecules during embryo development.

Software tools to perform evolutionary analyses on gene/protein sequences were developed: (1) IMPACT – a new GUI that estimates evolutionary rates on protein sequences by assessing changes in biochemical constraints, phylogeny and protein structure; and (2) TI2BioP (Topological Indices to BioPolymers) – an alignment-free approach that allows the calculation of the spectral moments as simple topological indices (TIs) to seek Quantitative Sequence-Function Relationships (QSFR) models, which we successfully applied to the study of bacteriocins, proteinaceous toxins produced by both gram-negative and gram-positive bacteria.

During 2010, we published a total of 25 papers in SCI journals, 1 book chapter, presented 18 oral communications and 31 posters in national and international conferences.


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8d. Productivity


1. Vasconcelos, V, Azevedo, J, Silva, M, Ramos, V. 2010. Effects of marine toxins on the reproduction and early stages development of aquatic organisms. Marine Drugs 8(1): 59-79.

2. Campos, A, Vasconcelos, VM. 2010. Molecular mechanisms of microcystin toxicity in animals. International Journal of Molecular Sciences 11: 268-287.

3. Lopes, V, Antunes, A, Martins, R, Welker, M, Vasconcelos, V. 2010. Morphological, toxicological and molecular characterization of a benthic Nodularia strain isolated from a Portuguese estuary. Research in Microbiology 161: 9-17.

4. Lopes, V, Fernandez, N, Martins, R, Vasconcelos, VM. 2010. Primary screening for bioactivity of brackishwater cyanobacteria: Ecotoxicity and embryotoxicity of crude extracts. Marine Drugs 8: 471-482.

5. Almeida, AM, van Harten, S, Campos, A, Coelho, AV, Cardoso, LA. 2010. The effect of weight loss on protein profiles of gastrocnemius muscle in rabbits: a study using 1D electrophoresis and peptide mass fingerprinting. Journal of Animal Physiology and Animal Nutrition 94(2): 174-185.

6. González-Díaz, H, Dea-Ayuela, MA, Pérez-Montoto, LG, Prado-Prado, FJ, Agüero-Chapín, G, Bolas-Fernández, F, Vazquez-Padrón, RI, Ubeira, FM. 2010. QSAR for RNases and theoretic-experimental study of molecular diversity on peptide mass fingerprints of a new Leishmania infantum protein. Molecular Diversity 14: 349-369.

7. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, F, Vasconcelos, V, Oudra, B. 2010. Effects of the microcystin profile of a bloom on the toxicity and toxin accumulation in common carp (Cyprinus carpio L.). larvae. Journal of Fish Biology 76:1415-1430.

8. Almeida, AM, Campos, A, Francisco, R, van Harten, S, Cardoso, LA, Coelho, AV. 2010. Proteomic investigation of the effects of weight loss in the gastrocnemius muscle of wild and New Zealand white rabbits via 2D-electrophoresis and MALDI-TOF MS. Animal Genetics 41: 260-272.

9. Fathalli, A, Jenhani, A, Saker, M, Moreira, C, Romdhane, MS, Vasconcelos, VM. 2010. First observation of the toxic and invasive cyanobacterium species Cylindrospermopsis raciborskii in Tunisian freshwaters. Toxicity assessment and molecular characterization. Fresenius Environmental Bulletin 19: 1074-1083

10. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, del Campo, FF, Oudra, B, Vasconcelos, V. 2010. Effect of different microcystin profiles on the toxins bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety 73:762-770.

11. Frazão, B, Martins, R, Vasconcelos, V. 2010. Are know toxins involved in the toxicity of picoplanktonic and filamentous North Atlantic marine cyanobacteria?. Marine Drugs 8: 1908-1919.


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12. Vasconcelos, VM, Vale, M, Martins, A, Antunes, A, Azevedo, J, Welker, M, Lopez, O, Montejano, G. 2010. First report on the occurrence of microcystins in planktonic cyanobacteria from Central Mexico analyzed by molecular, immunological and chemical methods. Toxicon 56: 425-431.

13. Ramos, V, Vasconcelos, V. 2010. Palytoxin and analogues: biological and ecological effects. Marine Drugs 8(7): 2021-2037.

14. Leão, PN, Pereira, AR, Liu, W-T, König, GM, Dorrestein, PC, Vasconcelos, VM, Gerwick, WH. 2010. Synergistic allelochemicals from a freshwater cyanobacterium. Proceedings of the National Academy of Sciences 107: 11183-11188.

15. Silva, P, Vasconcelos, V. 2010. Allelopathic effect of water contaminated with Cylindrospermopsis raciborskii on the germination and growth of several plant species. Chemistry and Ecology 26: 263-271.

16. Herfindal, L, Jokela, J, Myhren, L, Permi, P, Selheim, F, Wahlsten, M, Kleppe, R, Krakstad, C, Vasconcelos, V, Sivonen, K, Døskeland, SO. 2010. A novel nostocyclopeptide inhibits microcystin and nodularin induced apoptosis in hepatocytes. ChemBioChem 11: 1594-1599.

17. Campos, A, Carvajal-Vallejos, PK, Villalobos, E, Franco, CF, Almeida, AM, Coelho, AV, Torné, JM, Santos, M. 2010. Characterisation of Zea mays L. plastidial transglutaminase: interactions with thylakoid membrane proteins. Plant Biology 12: 708-716.

18. Stoichev, T, Basto, MCP, Vasconcelos, V, Vasconcelos, MTSD. 2010. Fate and effects of NP in the presence of the cyanobacterium Microcystis aeruginosa. Chemistry and Ecology 26: 395-399.

19. Nogueira, I, Lobo da Cunha, AL, Afonso, A, Rivera, S, Azevedo, J, Monteiro, R, Cervantes, R, Gago-Martinez, A, Vasconcelos, V. 2010. Toxic effects of domoic acid in the sea bream Sparus aurata. Marine Drugs 8: 2721-2732.

20. da Fonseca, RR, Kosiol, C, Vinar, T, Siepel, A, Nielsen, R. 2010. Positive selection on apoptosis related genes. FEBS Letters 584: 469-476.

21. Martins, J, Peixe, L, Vasconcelos, V. 2010. Cyanobacteria and Bacteria co-occurrence in a Wastewater Treatment Plant (WWTP): Absence of Allelopathic Effects. Water, Science and Technology 62(8): 1954-1962.

22. Marques, JRF, da Fonseca, RR, Drury, B, Melo, A. 2010. Conformational characterization of disulfide bonds: A tool for protein classification. Journal of Theoretical Biology 267: 388-395.

23. da Fonseca, RR, Johnson, WE, O’Brien, SJ, Vasconcelos, V, Antunes, A. 2010. Molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases. BMC Evolutionary Biology 10: 281.

24. Ramos, V, Seabra, R, Brito, Â, Santos, A, Santos, CL, Lopo, M, Moradas-Ferreira, P, Vasconcelos, V, Tamagnini, P. 2010. Characterization of a marine unicellular cyanobacterium


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that constitutes a separate clade together with thermophilic strains. European Journal of Phycology 44: 394-403.

25. Azul, AM, Ramos, V, Sales, F. 2010. Early effects of fire on herbaceous vegetation and mycorrhizal symbiosis in high altitude grasslands of Natural Park of Estrela Mountain (PNSE). Symbiosis 52: 113-123.


1. Agüero-Chapin, G, Sánchez-Rodríguez, A, Antunes, A, de la Riva GA, González Díaz, H. 2010. Chapter 5: Network entropies classification of fungi and bacteria of fungi and bacteria cellulases of interest for biotechnology. In: González-Díaz, H, Munteanu, CR (Eds). Topological Indices for Medicinal Chemistry, Biology, Parasitology, Neurological and Social Networks, Transworld Research Network, Kerala, India, pp. 69-95. ISBN 978-81-7895-989-9.



MASTER THESES

Salgado, Lídia. 2010. Desinfecção de águas naturais contaminadas com cianobactérias por fotocatálise solar. Master thesis in Environmental Engineering, Faculty of Engineering, University of Porto. Cu-supervisor: Vítor Vasconcelos, Rui Boaventura and Vítor Vilar (Faculty of Engineering, University of Porto).

Roldão, Mariana. 2010. Ecotoxicologia e Educação. Dos estudos no estuário do Rio Ave à elaboração de ferramentas educativas. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.

Silva, Marisa. 2010. Tetrodotoxin ocurrence in Portuguese coastal waters: first report on Atlantic coastal waters. Master thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.

Joussef, Samira. 2010. Evolutionary genetics of the AMT domain of the microcystin gene cluster in various cyanobacteria genera. Master thesis in Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: Agostinho Antunes, Co-supervisor: Vítor Vasconcelos.

PhD THESES

Leão, Pedro. 2010. Allelopathic properties of cyanobacteria isolated from Portuguese freshwaters. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.


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- 8th International Conference on Toxic Cyanobacteria, Istanbul, Turkey, 29 August – 4 September 2010 (V Vasconcelos – Member of Scientific Commission).

- 12º Encontro da Sociedade Portuguesa de Ecologia, SPECO, Porto, 18-20 October 2010 (V Vasconcelos – Member of Scientific Commission and Vice-president of Organizing Commission).

- ECClima - Estuaries in a changing climate – International Conference, CIIMAR, 5-8 April 2011 (V Vasconcelos – Member of Scientific Commission).



V Vasconcelos: Stephan Pflugmacher, IGB, Berlin, Germany; Stein-Oven Doskeland, Univ Bergen, Norway; Brahim Oudra, Univ Cadi Ayyad, Marrakesh, Morocco; Ana Gago, Univ Vigo, Spain; Amel Jenhani, INAT, Tunis, Tunisia; Jordi Molgo, CNRS, Paris, France, Gustavo Montejano, Lab Phycology, National Univ Mexico, Mexico; Luis Botana, Univ Santiago Compostela, Lugo, Spain.

Member of the Task Force - Marine and Freshwaters Toxins – of the Association of Official Analytical Chemists - AOAC International (2007-).

A Antunes: Stephen J O’Brien, Warren E Johnson, Shu-Jin Luo, Jennifer Troyer, Jill Pecon- Slaterry, Melody E Roelke, Lab of Genomic Diversity, NCI, NIH, MD, USA; Gustavo de la Riva, Sylvester Comprehensive Cancer Center, Univ Miami, Florida, USA; Sue VandeWoude, Sam Franklin, Colorado State Univ, CO, USA; Craig Packer, Univ Minnesota, USA; Laurence Frank, Univ California Berkeley, USA; Katherine C Prager, Univ California Davis, USA; David Wildt, Mitch Bush, Smithsonian’s National Zoological Park, Front Royal, USA; Kuo-Chen Chou, Gordon Life Science Inst, San Diego, USA; Eugenio Uriarte, ITESS, Guanajuato, Mexico; Humberto Gonzalez-Diaz, Florencio M Ubeira, Univ Santiago Compostela, Spain; Graham Hemson, Wildlife Conservation Research Unit, Tubney, Oxon, UK; Christiaan Winterbach, Hanlie Winterbach, Tau Consultants, Maun, Botswana; Kathy A Alexander, Department of Wildlife & National Parks, Kasane, Botswana; Philip Stander, Ministry of Environment & Tourism, Windhoek, Namibia; Ludwig Siefert, Makerere Univ, Kampala, Uganda; Margaret Driciru, Uganda Wildlife Authority, Kampala, Uganda; Paul J Funston, Tshwane Univ of Technology, Pretoria, South Africa; Gus Mills, Univ Pretoria, South Africa; Paolo Martelli, Ocean Park, Aberdeen, Hong Kong; Olga Uphyrkina, Inst Biology & Soil Sciences, Vladivostok, Russia; Aminael Sanchez-Rodríguez, Gisselle Pé rez-Machado, Reinaldo Molina-Ruiz, unierkis Pérez-Castillo, Aliuska Morales-Helguera, ReinaldoMolina-Ruiz, CBQ, IBP, Faculty of Chemistry and Pharmacy, UCLV, Santa Clara, Cuba; Philippe Gaubert, Muséum National d’Histoire Naturelle, Paris, France.


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A Campos: Mireya Santos, Josep Maria Torné, Center of Research in Agrogenomics – CRAG, CSIC-IRTA-UAB, Barcelona, Spain; Ana M Camean, Ana I Prieto, Faculty of Pharmacy, Univ Seville, Spain.


LEGE has been responsible for the analysis of water and counseling to many public health departments in what concerns the environmental and human health risk management associated with toxic cyanobacteria and other phytoplankton.

LEGE has been working in a national project coordinated by INAG (Portuguese Water Institute, Ministry of Environment) in the Water Framework Directive of Costal Waters. Responsible for the phytoplankton analysis of northern estuaries.

V Vasconcelos is also consultant with INAG in order to coordinate a national intercalibration exercise for phytoplankton analysis in freshwaters.

V Vasconcelos, as the scientific Coordinator of CMIA Vila do Conde until March 2010 (Interpretation and Monitoring Center of Vila do Conde) has directed the study of the physico-chemical and microbiological quality of the beaches of Vila do Conde area. A Report on the Water Quality of those beaches during 2008 was published and distributed to Public health Authorities, Municipality of Vila do Conde and CIMAR.

A Antunes as the scientific Coordinator of CMIA Matosinhos (Interpretation and Monitoring Center of Matosinhos) has launched the public reopening of this CMIA in August 2010 (http://www.cmia-matosinhos.net/), which included monthly seminars and public thematic exhibitions, plus several didactic activities to high school students and the general public.

Contracts with National authorities:

- Avaliação do estado ecológico das massas de água costeiras e de transição adjacentes e do potencial ecológico das massas de água fortemente modificadas. Funded by POVT/ Eixo III - Combate à Erosão Costeira, QREN, 2009-2010. Responsable: V Vasconcelos.Ensaio laboratorial de fitoplâncton de águas doces. Coordenação do exercício interlaboratorial nacional. Funded by INAG, 2009-2010. Responsable: V Vasconcelos.


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Environmental Technologies



Environmental Technologies


Maria Clara Semedo da Silva Costa




The funding sources of Environmental Technologies group of CCMAR in 2010 were:

- 4.302 EUR FCT Pluriannual funding.

- 2.750 EUR, partial budget given by FCT to the University of Algarve for the PhD scholarship of Mónica Sofia Furtado Martins, SFRH/BD/29677/2006.

- 1.850 EUR, Joint research Portugal- Tunisia protocol, funded by FCT and Ministry of High Education and Scientific Research and Technology of Tunisia, for missions between both countries.


1. Objectives

General objectives:

The central aim of the Environmental Technologies group is to contribute to the better knowledge and development of sustainable biotechnologies based on the use of anaerobic bacteria for the decontamination or recovery of metals from metals contaminated waters or effluents. The production of materials with useful applications (e.g. nanomaterials) instead of wastes as a consequence of the bioremediation processes is also aimed. The molecular identification of the bacteria involved, the metals-bacteria interactions and the dynamics of the bacterial communities are also objectives of the research developed by this group.

Specific objectives:

In the framework of Mónica Martins PhD


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The objectives of the fourth year of her PhD entitled “Bio-removal of toxic metals by metal resistant anaerobic bacteria: molecular characterization and performance studies” were:

- Phylogenetic analysis of a bacterial community developed in a bioremediation process for the treatment of acid mine drainage (AMD) using wine wastes as carbon source

- Investigation of bacterial community shifts during bioremediation of AMD from two Portuguese mines

- Phylogenetic characterization of a bacterial community resistant to aluminium (III)

- Screening of sulphate-reducing bacteria (SRB) resistant to bromate and with ability for bromate removal from aqueous solution

In the framework of João Costa PhD

The objectives of the second year of his PhD were:

- Synthesis of metallic sulphide nanoparticles (nanoparticles of zinc, copper and iron) using biologically generated sulphide

- Synthesis of metallic sulphide nanocomposites (nanocomposites of zinc, copper and iron sulphides) using TiO2 and SiO2 as supports using biologically generated sulphide

- Characterization of the synthesized nanoparticles and nanocomposites

In the framework of Maria Fernandes postdoc

- Multiple molecular characterization of a bacterial consortium isolated from an Icelandic fumarole displaying exceptionally high levels of sulphate reduction and metals resistance.


In the framework of Mónica Martins PhD

- The phylogenetic analysis of a bacterial community developed in a bioremediation process for the treatment of AMD using wine wastes as carbon source allowed the identification of bacteria affiliated with Desulfovibrio, Clostridium, Citrobacter and Cronobacter genera and with Bacteroidales order.

- The temporal evolution of the bacterial populations involved in two bioremediation processes where different AMD sources were used revealed that the AMD fed to the system was determinant for the establishment of different bacterial populations.

- Phylogenetic analysis of a bacterial community resistant to soluble Al(III) showed that it was mainly composed by bacteria closely related to Desulfovibrio desulfuricans (90%).

- Considering the increasing environmental restrictions imposed to the presence of bromate in water the search of bacteria highly resistant to this anion and with ability for


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its removal is a novel research topic of great interest. A SRB consortium isolated from sludge from a waste water treatment plant showed resistance to bromate and ability to simultaneously remove bromate and sulphate which is a very promising result.

In the framework of João Costa PhD

- We have successfully synthesized metallic sulphide nanoparticles (NPs), namely, ZnS and CuS, in the absence or presence of TiO2 or SiO2 as support, in batch, using biologically generated sulphide added to artificial solutions containing one, two or the three main metals present in AMD (Cu, Zn, Fe), as well as AMD. These NPs have been characterized using X-Ray Diffraction and Electron Microscopy (TEM and SEM), coupled to Energy-Dispersive X-Ray Spectroscopy. It has been established that the composition of the growth media and the presence of bacteria or particulates do not present great influence on the characteristics of the synthesized NPs.

In the framework of Maria Fernandes postdoc

- The molecular characterization of a bacterial consortium isolated from an Icelandic fumarole, that displayed unusual metabolic rates in the presence of Fe, Cu and Zn was achieved.

8d. Productivity


1. Martins, M, Faleiro, ML, Costa, AMR, Chaves, S, Tenreiro, R, Matos, AP, Costa, MC. 2010. Mechanism of uranium (VI) removal by two anaerobic bacterial communities. Journal of Hazardous Materials 184: 89-96.

2. Martins, M, Faleiro, ML, Chaves, S, Tenreiro, R, Costa, MC. 2010. Effect of uranium (VI) on two sulphate-reducing bacteria cultures from a uranium mine site. Science of the Total Environment 408: 2621-2628.

3. Martins, M, Faleiro, ML, Chaves, S, Tenreiro, R, Santos, ES, Costa, MC. 2010. Anaerobic bio-removal of uranium (VI) and chromium (VI): Comparison of microbial community structure. Journal of Hazardous Materials 176: 1065-1072.

4. Martins, M, Santos, ES, Pires, C, Barros, RJ, Costa, MC. 2010. Production of irrigation water from bioremediation of acid mine drainage: comparing the performance of two representative systems. Journal of Cleaner Production 18: 248-253.


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PhD THESES

Neves, Mónica. 2010. Bio-removal of toxic metals by metal resistant anaerobic bacteria: molecular characterization and performance studies. PhD thesis in Environmental Biotechnology, University of Algarve. Supervisor: Maria Clara Costa, Co-supervisor: Raul Barros.




- Costa, MC, Barros, RJ, Martins, MS, Jesus, C. Patente Nacional nº 103894, Uso de lama de mármore para pré-tratamento químico de efluentes ácidos, 06/08/2010.

- Costa, MC, Barros, RJ, Martins, MS, Jesus, C. Patente Nacional nº 104182, Uso de resíduos vinícolas como fonte de carbono e de energia para bactérias sulfato-redutoras, 22/11/2010.




Joint research Portugal-Tunisia protocol 2010, entitled “Study of microbial metal-removal to bioremediate non radioactive and radioactive wastes” funded by Portuguese Foundation for Science and Technology (FCT) and Ministry of High Education and Scientific Research and Technology of Tunisia. A research project aiming the application of metal-removal bacteria to bioremediate non radioactive and radioactive wastes such as phosphogypsum material existing in both countries was started in 2010 in the framework of Portugal-Tunisia protocol. In that context the PhD student Mónica Martins and the Environmental Technologies group leader, Maria Clara Costa, spent a period at the National Center for Nuclear Sciences and Technologies (CNSTN) for exchange of scientific experience. A PhD student from Tunisia and her supervisor Dr. Haïtham Sghaier, researcher at CNSTN, will be also received in the Environmental Technologies laboratory in 2011, also aiming the exchange of scientific knowledge.

The Environmental Technologies group has collaboration with Prof. José Miguel Nieto and Prof. Manuel Olias from Huelva University who have been working in the chemical treatment of acid


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mine drainage. Their groups have already implemented in situ pilot and field scale plants in the mines of Monte Romero and Esperanza, Spain, respectively. As a result of that collaboration a research project entitled “TRASAGUA – Sustainable treatment of acid mine drainage from the Iberian Pyrite Belt” and integrated in the RISE II Project was submitted to POCTEP programme (Programme for Portugal and Spain cooperation) by Algarve University, having the University of Huelva as a partner. That project aims the optimization of AMD treatment by the addition of a biological step, based on the use of sulphate-reducing bacteria in which the Environmental Technologies group has recognised expertise.



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Environmental Toxicology



Environmental Toxicology


Maria Armanda Reis Henriques




- 22.500 EUR FCT pluriannual funding.

- National funding

71.000 EUR, FCT PTDC/MAR/68106/2006, The modulation of retinoic acid signaling pathways by environmental pollutants in teleosts. 09/2008-08/2011. PI: M Santos.

45.652 EUR, FCT PTDC/MAR/098791/2008, The role of ABC transporter proteins in chemically induced hepatic cancer in zebrafish (Danio rerio). 04/2010-03/2013. PI: M Ferreira.

55.260 EUR, PTDC/AGR-AAM/102316/2008, Cephalopods:-Benefits and risks of consumption; - Evaluation of biomarkers responses to organic pollution. 2010-2013. PI: MA Henriques.

64.500 EUR, FCT PTDC/MAR/105199/2008, The invertebrate repertoire of nuclear receptors: evolutionary and endocrine disruption insights. Responsible partner at LETOX: M Santos.

- European funding

265.120 EUR, INTERREG IV Atlantic Area, ARCOPOL – The Atlantic Region’ Coastal POLlution Response. 01/2009-12/2011. Partner Scientific Leader: MA Henriques.


1. Objectives

The research of the Environmental Toxicology Group (LETOX) in 2010 focused in several areas.


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In the field of endocrine disruption the main aim is to improve environmental risk assessment of endocrine disrupting chemicals by using a multiparametric approach which combines molecular biology and ecological methodologies in order to understand the mechanisms of toxicity of endocrine disrupting chemicals, single and in mixtures, and their impact in the aquatic ecosystems. Since the genomic make up of aquatic animals varies considerably, our approach takes in consideration an evolutionary perspective thus focusing in the impact of EDCs across different phyla. The EDCs of interest include androgenic, anti-androgenic and estrogenic chemicals and well as emergent pollutants such as pharmaceuticals. Since the endocrinology and the genomic make up of most invertebrates are poorly characterized, we aim at improving the understanding of invertebrate’s endocrinology and link it with the impact of EDCs in key signaling pathways.

The research area concerning pharmaceuticals in the aquatic environment is a new objective. Among the quantity of pharmaceuticals sold, psychopharmaca comprise a major group in terms of sales – particularly in Portugal. Since they specifically target neurotransmitters in the brain, severe impact on vital functions such as the endocrine regulation has to be assumed at low concentrations. In an environmental monitoring study, prevailing psychopharmaceuticals in a hot spot estuary (Douro) are identified and subsequently considered as model substances to reveal their impact on the main hormone systems (reproduction, osmoregulation, growth and development) in fish. Assessing the neuroendocrine disruption will not just provide sophisticated data for environmental risk assessment but allows for new insights on neuroendocrinology and the interlinkage of hormone systems, contributing to a better understanding of fish endocrinology. In parallel, we continued our research on the mechanisms of detoxification of organic contaminants including research on efflux membrane transporters in fish, the multidrug resistance pump P-glycoprotein (Pgp) and the multridrug resistance-associated proteins (MRPs) involved in the multixenobiotic resistance (MXR), or the newly called phase 0 and phase III of detoxification. Moreover, these ABC transporter proteins are involved in the resistance to chemotherapy in human tumours, while in fish this subject remains less explored. In the field, fish exposed to pollutants have shown to develop tumours, and by chemically inducing liver tumours in zebrafish we have the goal to increase the knowledge on the role of these transmembrane transporters in tumours and their protective role by effluxing toxic chemicals from cells. While in a medical approach the goal is to inhibit ABC transporters, in an ecotoxicological approach the ideal situation is to have the efflux proteins working properly in order to protect aquatic organisms. Other topic of research is the study of the potential toxic effects of PCBs and PAHs, as well as the toxicity of their metabolites in aquatic organisms included in human diet to obtain a better understanding of their body distribution. Juvenile gilthead seabream and commum octopus, both marine species with high lipid content and ecological relevant, are studied by the evaluation of different endpoints, as the levels of the biotransformation and oxidative stress enzymes, DNA damage and endocrine effects to give the toxic effects and the possible transfer of these chemicals through the food chain.

Another line of research aims at improving the prevention, response and mitigation in the scope of the European project ARCOPOL with the objective to establish methodology for environmental damage assessment, based on hazard evaluation of the most relevant HNS.

In the scope of the surveillance monitoring programs a new task started at LETOX, which aims to establish scientific and valid criteria allowing the classification of the different types of water bodies of transition in southern Galicia and northern Portugal, in international waters of Minho


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estuary. CIIMAR and LETOX are one of the members of the Team-Minho project, and have the responsibility to develop biological responses (bioindicators and biomarkers) to be integrate in investigative monitoring programs for the implementation of Water Framework Directive.


In the scope of ED, we continued an innovative approach which aims the understanding of the effects of antagonistic EDCs under complex mixtures in fish. We demonstrated that mixture effects of androgenic and estrogenic chemicals induce unpredictable effects in comparison with single exposure. This finding is essential to improve environmental risk assessment of chemical mixtures of EDCs acting through dissimilar mode of action. In contrast to most studies focusing on effects of EDCs in reproduction, we initiated a new research area dealing with the effects of these chemicals in larvae/juvenile. Our data indicates that androgenic chemicals, estrogenic chemicals, modulators of retinoic acid signaling pathways as well as other pharmaceuticals induce subtle changes in ecological relevant endpoints such as behaviour and embryonic development, which are often missed in most studies, but may have important implications in survival during the juvenile phase, and hence impact population dynamics. In the field, we have validated the use of a new sentinel species, the shanny Lypophris pholis, for estrogenic chemical’s pollution monitoring. We continue the isolation of several nuclear receptors in invertebrates, and, at the moment, we are testing the functionality of such signalling pathways, and whether they are prone to disruption by several environmental chemicals. We have demonstrated that the nuclear receptor RXR is a key player in imposex induction by the androgenic chemical tributyltin.

We started a project that focuses on the role of the efflux ABC transporters in chemically induced hepatic tumours using zebrafish (Danio rerio) as the model species. In humans, these transmembrane transport proteins are responsible for the resistance to chemotherapy, phenomenon called multidrug resistance (MDR). In fish a parallel mechanism has been described, the multixenobiotic resistance (MXR), however the role of ABC transporters in fish hepatic tumours remains uncertain and with this project we hope to achieve a better knowledge in this resistance mechanism. We have now cloned and sequenced several ABC transporter genes in two important model species, the European seabass (Dicentrarchus labrax) and Nile tilapia (Oreochromis niloticus), as well as gene expression of the mention genes in different conditions of exposure to PAH in Nile Tilapia. With the last referred work we were able to bring new knowledge on the possible cooperation of the efflux proteins with biotransformation enzymes, in the overall detoxification mechanism. Moreover, MXR activity assays and biotransformation enzymes, phase I and phase II, in seabass primary cultured hepatocytes have been proven to be fully functional and this new in vitro system can become a useful tool to screen pollutants effects, at the detoxification level, in a marine species. One important conclusion was obtained by the exposure of juvenile Nile tilapia to benzo(a)pyrene in water and in diet that showed different metabolization pathways according to the different exposure routes, and moreover the different tissue distribution of the carcinogenic BaP metabolite, the 3-hydroxy benzo(a)pyrene.

We were able to measure for the first time biomarkers of exposure in octopus (Octopus vulgaris), mainly oxidative stress biomarkers such as antioxidant enzyme activities and oxidative damages in lipids and proteins, which will be useful data in order to validate this as a


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bioindicator species for monitoring purposes, and also assess its quality for human consumption by measuring oxidative damages in the consumed part of the body.

The work developed under ARCOPOL has released a bibliographic review of ecological risk assessment methodologies and derivations of PNECs (Predictive No Effect Concentration) for marine species. We validated the use of the shanny L. pholis for pollution monitoring of both chronic and spill oil pollution, demonstrating, in the field, the impact of a moderate oil spill of the Petrogal refinery. Based on the list of 12 HNS previously selected, an approach in monitoring HNS contamination, based on the biological responses at individual level by measuring physiological markers and biomarkers of exposure/effect were developed in the crustacean Artemia franciscana and the amphipode Gammarus exposed to different concentrations of xylene the first HNS of the list to be studied.

8d. Productivity


1. Ferreira, F, Santos, MM, Reis-Henriques, MA, Vieira, NM, Monteiro, NM. 2010. Sexing blennies using genital papilla morphology or ano-genital distance. Journal of Fish Biology 77: 1432-1438.

2. Ferreira, M, Caetano, M, Antunes, P, Costa, J, Gil, O, Bandarra, N, Pousão-Ferreira, P, Vale, C, Reis-Henriques, MA. 2010. Assessment of contaminants and biomarkers of exposure in wild and farmed seabass. Ecotoxicology and Environmental Safety 73: 579-588.

3. Santos, MM, Solé, M, Lima, D, Hambach, B, Ferreira, AM, Reis-Henriques, MA. 2010. Validating a multi-biomarker approach with the shanny Lipophrys pholis to monitor oil spills in European marine ecosystems. Chemosphere 81: 685-691.

4. Trubiroha, A, Kroupova, H, Wuertz, S, Frank, SN, Sures, B, Kloas, W. 2010. Naturally-induced endocrine disruption by the parasite Ligula intestinalis (Cestoda) in roach (Rutilus rutilus).General and Comparative Endocrinology 166: 234-240.

In press

5. Lima, D, Reis-Henriques, MA, Silva, R, Santos, AI, Castro, FC, Santos, MM (in press). Tributyltin-induced imposex in marine gastropods involves tissue-specific modulation of the Retinoid X Receptor. Aquatic Toxicology.

6. Neuparth, T, Moreira, S, Santos, MM, Reis-Henriques, MA (in press). Hazardous and Noxious Substances (HNS) in the marine environment: prioritizing HNS that pose major risk in a European context. Marine Pollution Bulletin.

7. Sárria, MP, Santos, MM, Reis-Henriques, MA, Vieira, NM, Monteiro, NM (in press). The unpredictable effects of mixtures of androgenic and estrogenic chemicals on fish early life. Environment International.


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8. Costa, PM, Neuparth, T, Caeiro, S, Lobo, J, Martins, M, Ferreira, AM, Caetano, M, Vale, C, Delvalls, TA, Costa, MH (in press). Assessment of the genotoxic potential of contaminated estuarine sediments of fish peripheral blood: Laboratory versus in situ studies. Environmental Research.


1. Santos, MM, Reis-Henriques, MA, Castro, LFC (in press). Lipid homeostasis perturbation by organotins: effects on vertebrates and invertebrates. In: Pagliarani, A., Trombetti, F, Ventrella, V (Eds). Biochemical and biological effects of organotins, Chapter 6. Beham Science Publishers, Ltd







Spain

- Institut de Ciences del Mar - CSIC, Dr. Montserrat Sóle; the collaboration deals with the effects of oil pollution in fish and invertebrates.

- Instituto Tecnolóxico para o Control do Medio Mariño de Galicia (INTECMAR).

Germany

- Federal Institute of Hydrology (Koblenz), Thomas Ternes, Guido Fink; Chemical analysis of pharmaceutical residues in the aquatic environment.

- Institute of Freshwater Ecology and Inland Fisheries (Berlin), Prof. Werner Kloas, Ilka Lutz; Expression analysis of single cells (Laser dissection microscope, qPCR), two ongoing projects (“Parasites as endocrine disruptors”, “Reproductive physiology of Sander”).


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Italy

- ENEA Centro Ricerche Casaccia, Roberto Morabito and Paolo Massanisso; we seek to understand the effects of pollutants with endocrine disrupting properties, in sexual development and reproduction of fish.

- Dipartimento di Scienze Ambientali, Università Ca’ Foscari Venezia.

Tunisia

- University of Bizerte, Laboratory of Environment Bio-monitoring, Department of Biology; the collaboration focus on the effects of EDCs in molluscs.

Japan

- Osaka University, Department of Toxicology, Prof. Junichi Nishikawa; Together with this group, we try to better understand the effects of EDCs in invertebrates, through modulation of nuclear receptors. This collaboration has already resulted in one publication in a reputable international journal.

Croatia

- Rudjer Boskovic Institute, Zagreb, Laboratory for Molecular Ecotoxicology, Tvrtko Smital; purpose to increase the knowledge on the detoxification mechanisms in aquatic species, by studying phase 0 and III.

India

- National Institute of Oceanography, Dona Paula, Goa; To evaluate the impact of the more representative organic pollutants in some species of fish living in estuaries and coastal zones.

France

- Conseil Régional d’Aquaculture, Conseil Régional de Bretagne, Conseil Régional des Pays de la Loire, Conseil Régional de Poitou Charentis.

United Kingdom

- Department of Biological Sciences, Brunel University, Centre for Toxicology, The School of Pharmacy, University of London, Devon Country Council, Dorset Country Council, Pembrokeshire County Council, Environmental and Heritage Services, Devon Wildlife Trust.


LETOX participates in ARCOPOL - The Atlantic Regions Coastal Pollution Response, a project funded by Interreg IV Atlantic Area, the transnational territorial cooperation program between regions of the Atlantic Area. This project aims to improve the preparedness, response and


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mitigation capabilities of local responders to accidental coastal pollution specifically against oil, hazardous and noxious substances and inert spills. It involves the participation of regional and national authorities (National Maritime Authority of the Ministry of National Defence and Port Authority in Portugal) assuring the effective involvement in public policy advice.


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Evolution, Development and Gene Expression (EDGE)



Evolution, Development and Gene Expression (EDGE)


Maria Leonor Quintais Cancela Fonseca





- 19.333 EUR FCT Pluriannual funding

- National funding

24.169 EUR, FCT, PTDC/CVT/72083/2006, ISOPERK.

77.784 EUR, FCT, PTDC/MAR/105313/2008, FISHCELL.

63.547 EUR, FCT, PTDC/MAR/105152/2008, SPECIALK.

- European funding

40.000 EUR, EU/FP7, ASSEMBLE-2008-227799.

- Collaborative actions

COST ACTION FA0801, LARVANET (no budget).

- Industrial contracts

10.000 EUR, NOVOZYMES (Denmark).

- Collaborations with local schools and city halls

30.000 EUR, DREA – UALG – Lab-IT, 12/2008-11/2012.


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- Prizes

50.000 EUR, Programa na Fronteira das Ciências da Vida, Prize awarded by the Calouste Gulbenkian Foundation, 2009-2012.


1. Objectives

I. Molecular determinants of tissue mineralization

- Develop in vitro cell systems to analyze mineralization-related gene expression/regulation and signaling pathways in fish.

- Develop in vivo systems to analyze gene expression and unveil de novo bone formation and regeneration pathways in fish.

- Develop fish-based screening methods to identify novel osteogenic molecules with therapeutic potential.

- Implement zebrafish as a model system to study bone-related human pathologies.

- Identify proteins involved in normal and abnormal skeletal development and calcium metabolism, and study their gene expression/regulation. Establish relevance for human health and disease.

- Determine microRNAs involved in fish skeletogenesis and in vitro mineralization, and identify target gene(s).

- Develop fish embryonic stem cells for the production of transgenic animals and to achieve differentiation into various mesenchyme derived lineages.

- Explore the mechanisms of action of nutrients (e.g. vitamins and fatty acids) on skeletal ontogenesis and development of anomalies.

II. Perkinsus-bivalve interactions

- Determine Perkinsus infection prevalence in bivalves along Portuguese coast and identify permissive/non-permissive species.

- Identify environmental parameters affecting Perkinsus survival/proliferation/infection.

- Develop molecular diagnostic tools to evaluate bivalve infection by Perkinsus.

- Develop genomic tools to identify Perkinsus/clam genes & metabolic pathways involved in parasite infection


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III. Population genetics

- Development of molecular tools suitable for paternity and genotyping wild populations for conservation & management purposes, as well as for implementation of new aquaculture species of interest.

IV. Analysis of microbial/invertebrate genomes to unveil possible targets for biotechnological applications

- Identification of AMP’s (Anti Microbial peptides).

- Identification and expression of hydrolases of GH9 family.

- Sequencing of an entire new virus family.


I. Molecular determinants of tissue mineralization

- Development of new mineralogenic cell lines from seabream, zebrafish, salmon, sturgeon and Xenopus, and ES cell lines from seabream blastula.

- Purification & cloning of fish bone Gla proteins. Evidence for two isoforms for OC and GRP in fish; Characterization of their evolutionary relationship.

- Analysis of the promoter activity of a variety of bone- and cartilage-related genes (e.g. SDR, FHL2, BMP2, MEF2C, RUNX3); Identification of transcription factor binding sites.

- Identification of signaling pathways (e.g. MAPK and PI-3K) regulating in vitro mineralization of fish bone-derived cell lines.

- Implementation of new zebrafish rearing and microinjection facilities.

- Identification of a microRNA affecting post-transcriptional expression of BMP2 and mineralogenic capacity of fish cell lines.

- Characterization of fish skeletal deformities by immuno-histomorphological approaches.

- Characterization of bone structure in deep-sea fish.

- Characterization of the role of seabream FHL2 in craniofacial muscle development and its action on cell division and differentiation.

II. Perkinsus-bivalve interactions

- Development of a clonal culture of Perkinsus olseni and in vitro testing of drugs (e.g. antimalarial) and ex vivo clam infection to identify environmental conditions affecting parasite proliferation/survival.

- Optimization of PCR-ELISA-based assay to determine Perkinsus levels & species.


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- Identification through functional genomics of target metabolic pathways (e.g. shikimate, folate) to control Perkinsus infection in hatcheries.

- Sequencing of clam and Perkinsus transcriptome and completion of Perkinsus genome.

III. Population genetics

Development & optimization of:

- Molecular markers (microsatellites) for Dusky grouper for management stock studies and offspring assignment;

- Paternity and genetic diversity analyses to determine differential contribution from brooders in specific populations of Senegalese sole (management and aquaculture).

8d. Productivity


1. Anjos, A, Leite, RB, Shahbazkia, HR, Cancela, ML. 2010. MAQ, a bioinformatics tool for automatic macroarray analysis. International Journal of Computer Applications 4(3): 1.

2. Bensimon-Brito, A, Cancela, ML, Huysseune, A, Witten, PE. 2010. The zebrafish (Danio rerio) caudal complex - a model to study vertebral body fusion. Journal of Applied Ichthyology 26(2): 235-238.

3. Cancela, ML, Witten, PE. 2010. Advances in skeletal biology by understanding the fish skeleton: a multidisciplinary challenge. Journal of Applied Ichthyology 26(2): 147-147.

4. Conceição, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, PJ, Mira, S, Dias, J. 2010. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry 36: 1-16.

5. Conceicao, N, Cox, CJ, Simões, B, Viegas, M, Cancela, ML. 2010. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology 26(2): 245-250.

6. Cunha, ME, Quental-Fereira, H, Boglione, C, Palamara, E, Gavaia, P, Pousão-Fereira, P. 2010. Rearing fish larvae for extensive and semi-intensive aquaculture - the “natural” mesocosms system. Aquaculture Europe 35(2): 23-26.

7. Dâmaso-Rodrigues, ML, Pousão-Ferreira, P, Ribeiro, L, Coutinho, J, Bandarra, NM, Gavaia, PJ, Narciso, L, Morais, S. 2010. Lack of essential fatty acids in live feed during larval and post larval rearing: effect on the performance of juvenile Solea senegalensis. Aquaculture International 18(5): 741-757.

8. Fazenda, C, Simões, B, Kelsh, RN, Cancela, ML, Conceição, N. 2010. Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene 450(1-2): 94-102.


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9. Louro, B, Passos, ALS, Souche, EL, Tsigenopoulos, C, Beck, A, Lagnel, J, Bonhomme, F, Cancela, L, Cerda, J, Clark, MS, Lubzens, E, Magoulas, A, Planas, JV, Volckaert, FAM, Reinhardt, R, Canario, AVM. 2010. Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: Characterization, tissue-specific expression and gene markers. Marine Genomics 3(3-4): 179-191.

10. Rafael, MS, Marques, CL, Parameswaran, V, Cancela, ML, Laizé, V. 2010. Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology. Journal of Applied Ichthyology 26: 230-234.

11. Rosa, JT, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2010. Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells. Journal of Applied Ichthyology 26: 245-250

12. Simão, MF, Leite, RB, Rocha, C, Cancela, ML. 2010. Changes in bioturbation of iron biogeochemistry and in molecular response of the clam Ruditapes decussates upon Perkinsus olseni Infection. Archives of Environmental Contamination and Toxicology 59: 433-443.

13. Viegas, CSB, Conceição, N, Fazenda, C, Simes, DC, Cancela, ML. 2010. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon (Acipenser naccarii). Journal of Applied Ichthyology 26 (2): 214-218.


1. Cancela, ML, Bargelloni, L, Boudry, P, Boulo, V, Dias, J, Huvet, A, Laizé V, Lapègue, S, Leite, R, Mira, S, Nielsen, EE, Planas, JV, Roher, N, Sarropoulou, E, Volckaert, FAM. 2010. Genomic approaches in aquaculture and fisheries. In: Cock, JM, Viard, F, Tessamar-Raible, K, Boyen, C (Eds). Introduction to Marine Genomics, Springer-Verlag, Heidelberg, pp. 213-286. ISBN 978-90-481-8616-7.



MASTER THESES

Simão, Márcio. 2010. Identification and characterization of two different transcripts of ferritin in the clam R. decussatus. Expression of the corresponding isoforms to evaluate some of their biological properties. Master thesis in Molecular and Microbial Biology, Faculty of Sciences and Technology, University of Algarve. Supervisor: Leonor Cancela, Co-supervisor: Ricardo Leite.

PhD THESES

Viegas, Carla. 2010. Identification and characterization of a new calcium binding protein from sturgeon, Gla rich protein or GRP. PhD thesis in Biochemistry, University of Algarve. Supervisor: M. Leonor Cancela, Co-supervisor: Dina C. Simes.


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- Identification of compound that affects osteogenesis, involves inducing fin regeneration of ray-finned fish by amputating fish fin, contacting fish with test compound, and determining re-mineralization index of fin regeneration. Patent Number(s): WO2010044689-A2; WO2010044689-A3. Inventor(s): Brito AB, Cancela ML, Gavaia PJ, Laizé V. Date: 22Abril 2010 and 10 June 2010.

- New isolated polypeptide having organophosphorous hydrolase activity selected from polypeptide containing amino acid sequence, polypeptide encoded by polynucleotide, variant containing deletion of amino acids, used in nucleic acid construct. Patent Number(s): WO2010128115-A1; EP2248893-A1. Inventor(s): Danielsen S, Skov LK, Leite R, Laizé V, Cancela ML. Date: 11 November 2010.

- New isolated polypeptide with organophosphorus hydrolase activity selected from e.g. polypeptide with amino acid sequence having specific identity to mature polypeptide and polypeptide encoded by polynucleotide, used in such as composition. Patent Number(s): WO2010128116-A1. Inventor(s): Danielsen S, Skov LK, Leite R, Laizé V, Cancela ML. Date: 11 November 2010.

- A gamma-carboxyglutamate-rich protein, methods and assays for its detection, purification and quantification and uses thereof. Patent Number(s): WO2010024704. Inventor(s): Simes DC, Viegas CSB, Cancela ML. Date: 03 March 2010.


- Seminars of PhD students from Biomedical Sciences Program, 18 November 2010, Faro, Portugal. Cancela ML - Coordinator.

- 4th Clinical Biochemistry Workshop, 29 January 2010 Faro, Portugal. A Alves, L Cancela, J Martins, R Leite - Organizing Committee.

- National Congress from the Portuguese Society of Biochemistry, 15-17 December 2010, Porto, Portugal. Cancela ML - Member of the Scientific Committee and co-organizer of the symposium Gene Expression and Disease.


- Novozyme SA (Denmark): Research collaboration aiming at the identification of genes

with biotechnological applications. Construction of cDNA libraries for Illumina

sequencing and posterior assemblage and gene identification.

- Technophage SA (Portugal): Research collaboration aiming at the discovery of new

molecules with therapeutic potential for human bone-related diseases. Proof-of-

concept experiments.

- SM Nutritional Products (Switzerland): Research collaboration aiming at investigating


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the effect of HyD on in vitro mineralization and in vivo bone formation. Partnership with

AquaGroup.

- SGS ICS (Portugal) Gavaia PJ was contracted as Scientific expert consultant for auditing

the Oceanarium of Lisbon in the process of renewing NP EN ISO 9001:2000, NP EN ISO

14001:2004 and EMAS.


Research

- EU/FP7-ASSEMBLE-2008-227799. EDGE is leading work package 14 (Improvement of provision of marine animal cell cultures/lines and tools for functional genomics); EDGE: 108.890 EUR.

- EU-ALW-GO-MG/10-07 /Bone cell mechanics under altered gravity /MechanoCell 2010-2014. Coordinator: JJWA Van Loon. EDGE participates with fish mineralogenic cell lines and expertise.

- EU/FP6-SEACASE-2005-SSP5A. EDGE studies skeletal malformations as potential markers to differentiate fish reared in different systems (EDGE: 160.00 EUR).

- EU/FP7-PROSPAWN-SME-2008-1. EDGE evaluates Senegalese sole parental contributions to F1 and inbreeding levels in F1 crosses (EDGE: 22.800 EUR).

- POCTEP-0251-ECOAQUA. EDGE involved in development of markers to assess broodstock contributions to progeny/evaluate different male contribution in competition conditions (EDGE: 50.000 EUR).

- COST ACTION/FA0801/Larvanet. Coordinator: Amos Tandler. Aims to improve scientific knowledge underlying sustainable aquaculture. Participants from 13 countries.

- Project evaluation (e.g. French National Research Agency (ANR), and European Research Council (ERC), referees (over 15 journals).

- Collaborative actions with Spain (FCT, CRUP, Spanish Ministry of Science) (2).

- Oral communications + plenary lectures at international conferences (4).

- Leite R. External consultant for Fungal screening, AMPs discovery and Next Generation Sequencing in Novozymes (DK).

Training (2010)

- Training: 3 MSc & 9 PhD students, 4 post-docs and international students.

- Teaching: undergraduate ERASMUS/SOCRATES students in ongoing University degrees.

- Hosting: visiting researchers from Norway, Belgium, Spain, France, UK and Canada.


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- Coordination (ML Cancela): (i) master course on Biology of Bone and Cartilage (Master degree in Biomedical sciences, UALG). Teaching involved lecturers from France, Germany and Belgium. (ii) PhD Program in Biomedical Sciences (includes students from Brazil & Mexico)

Research dissemination

- 13 papers in international peer-reviewed journals, 1 chapter (edited by ML Cancela) in international book, 24 communications in international meetings, 1 invited lecture (MLC, NOVOZYMES Company, Denmark), organization of 2 international symposiums/workshops.


Cancela ML (since January 2009) - President of the Board of the Assembly of the Portuguese Biochemistry Society, a non-profit, nationally recognized organization promoting the development and dissemination of biochemistry in Portugal. Of particular relevance has been the collaboration in the organization of a special interest meeting (Clinical Biochemistry, Faro Jan 2010) and promoting Biochemistry and Molecular Biology education in local high schools through seminars, training of high school teachers and implementation of laboratory classes.

Cancela ML - National representative of the Portuguese Society of Biochemistry in the International Union of Biochemistry and Molecular Biology (IUBMB) and the Pan American Biochemistry and Molecular Biology Association (PABMB).


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Fish Nutrition



Fish Nutrition


Aires Manuel Pereira Oliva Teles





- National funding

501.203 EUR, Sistema de Incentivos à I&DT - FEDER - Quadro de Referência Estratégico Nacional 2007-2013, OPTISOLE - Investigação e desenvolvimento na produção de juvenis de linguado: incremento da resistência a patologias e do crescimento. Participating Institutions: FCUP, ICBAS, CIIMAR, ACC, SPARUS. Participants of the Laboratory of Nutrition: A Oliva Teles, H Peres, AP Carvalho, I Guerreiro. 2008-2011.

5.000 EUR, University of Porto - Pluridisciplinary projects, Valorização do excedente de levedura da indústria cervejeira – potencial de utilização em dietas para os juvenis de linguado (Solea senegalensis). Participating institutions: FCUP, CIIMAR, IBMC. Participants of the Laboratory of Nutrition: A Oliva Teles, H Peres. 2010-2011.

4.000 EUR, University of Porto - Pluridisciplinary projects, Variações sazonais no estado nutricional da solha-das-pedras Platichthys flesus no estuário do rio Minho. Participating institutions: FCUP, CIIMAR. Participants of the Laboratory of Nutrition: P Carvalho.

188.980 EUR, FCT PTDC/MAR/68106/2006, The modulation of retinoic acid signalling pathways by environmental pollutants in teleosts. Participating institutions: CIIMAR, ICETA. Participants of the Laboratory of Nutrition: AP Carvalho. 2007-2010.


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1. Objectives

The Fish Nutrition group research is directed to the study of fundamental and applied aspects of the nutrition and feeding of larvae and juveniles of fish species well established in marine aquaculture (sea bass, sea bream, turbot), candidate species for aquaculture (sargus sp., sole, meagre), and ornamental species (zebrafish, angelfish, kribensis, jewelfish).

Main research activities are directed towards the study of:

- Protein and amino acid requirements, metabolism and utilization efficiency;

- Utilization of dietary lipids and carbohydrates;

- Phosphorus requirement and bioavailability;

- Potential of feedstuffs alternative to fish meal as dietary ingredients;

- Nutritional strategies for health and welfare improvement of fish;

- Development of microparticulate diets and weaning strategies for fish larvae.

Studies aim contributing for a deeper insight on nutritional requirements and of metabolic utilization of nutrients by fish, therefore gaining basic information regarding the physiological aspects of comparative animal nutrition and contributing for a scientific based improvement of fish diets. From an applied perspective, studies aim maximization of fish growth and feed utilization while reducing the environmental impact due to feeding. Evaluation of potential utilization of plant feedstuffs and other feedstuffs such as yeast aims partial or total replacement of dietary fish meal aiming to reduce pressure on fisheries and decrease fish feeds dependence on this limited commodity. This way it is expected to contribute for the sustainable development of aquaculture.


During the present year research was very much focused on studying the regulation of glucose metabolism in European sea bass (Publications nr 5 and 9). Also, the growth and feed utilization of European sea bass and gilthead sea bream fed diets including carbohydrates of different complexities was also evaluated (Publications nr 6 and 7).

The effect of two temperatures and of the dietary protein to lipid ratio in Senegalese dole juveniles was also studied (Publications nr 8). This research will be prosecuted in the next year using two other temperatures and different fish sizes aiming to establish the most adequate temperature for Senegalese sole juvenile growth and feed utilization as well as to establish the best protein to lipid ratio of the diets. Based on this information it is expected to establish a growth model for the species.

The work on potential evaluation plant feedstuffs for incorporation in diets for juveniles was also continued. The study of pea seed meal potential was pursued by evaluating the response of


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common carp to diets including pea seed meal subject to different thermal processing methods (Publications nr 2) and the digestibility of selected feed ingredients for tilapia (Publications nr 10) was also evaluated. A trial on the effect of purified antinutrients (saponins and phytoestrogens) in sea bass (Dicentratchus labrax) juveniles was performed within the framework of Ana Couto Phd. Thesis, and the partial or total replacement of fish meal by plant feedstuffs mixture in white sea bream diets was analysed within the context of Inês Cardoso MsC Thesis. Still running are studies on the effect of taurine supplementation in plant protein based diets for white sea bream (Carolina Oliveira MsC Thesis) and the nutrient and amino acids digestibility of plant feedstuffs in meager (Carla Olim MsC Thesis).

The analysis of different feed restriction protocols on growth performance and compensatory growth of turbot under commercial rearing conditions was also evaluated (Publications nr 1). Also, the effect of feeding regime (daily, night, or mixed) in growth, feed utilization and N excretion of Senegalese sole juveniles was also evaluated within the framework of Gonçalo Marinho MsC Thesis. This information is of immediate practical application for the management of feeding in turbot aquaculture.

Studies on the toxicological effect of algal blooms in fish larvae was also continued (Publications nr 3 and 4).

A review of the state of art on nutrition and feeding of sparids was prepared (Other Publications nr 1) and another review on the utilization of carbohydrates by European sea bass and gilthead sea bream was also prepared and submitted for publication.

8d. Productivity


1. Blanquet, I, Oliva-Teles, A. 2010. The effect of feed restriction on performance of turbot (Scophthalmus maximus) juveniles under commercial rearing conditions. Aquaculture Research 41: 1255-1260.

2. Davies, SJ, Gouveia, A. 2010. Response of common carp fed diets containing an isogenic pea seed meal (Pisum sativum) subjected to different thermal processing methods. Aquaculture, 305(1-4): 117-123.

3. El ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, del Campo, FF, Oudra, B, Vasconcelos, V. 2010. Effect of different microcystin profiles on the toxins bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety 73:762-770.

4. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, F, Vasconcelos, V, Oudra, B. 2010. Effects of the microcystin profile of a bloom on the toxicity and toxin accumulation in common carp (Cyprinus carpio L.). larvae. Journal of Fish Biology 76:1415-1430.

5. Enes, P, Sanchez-Gurmaches, J, Navarro, I, Gutiérrez, J, Oliva-Teles, A. 2010. Role of insulin and IGF-I on the regulation of glucose metabolism in European sea bass (Dicentrarchus labrax)


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fed with different dietary carbohydrate levels. Comparative Biochemistry and Physiology 157A: 346-353.

6. Enes, P, Peres, H, Couto, A, Oliva-Teles, A 2010. Growth performance and metabolic utilization of diets including starch, dextrin, maltose or glucose as carbohydrate source by gilthead sea bream (Sparus aurata) juveniles. Fish Physiology and Biochemistry 36: 903-910.

Accepted/in press

7. Enes, P, Couto, A, Peres, H, Oliva-Teles, A. (accepted). Growth, feed utilization and glycaemic responses in European sea bass Dicentrarchus labrax juveniles fed carbohydrate of different complexities. Journal of the World Aquaculture Society.

8. Guerreiro, I, Peres, H, Cunha, M, Oliva-Teles, A. (accepted). Effect of temperature and dietary protein/lipids ratio on growth performance, feed utilization and body composition of juvenile Senegalese sole (Solea senegalensis). Aquaculture Nutrition.

9. Enes, P, Peres, H, Sanchez-Gurmaches, J, Navarro, I, Gutiérrez, J, Oliva-Teles, A. (accepted). Insulin and IGF-I response to a glucose load in European sea bass (Dicentrarchus labrax) juveniles. Aquaculture.

10. Davies, SJ, Abdel-Warith, AA, Gouveia, A. (in press). Digestibility characteristics of selected feed ingredients for developing bespoke diets for tilapia culture in Europe and North America. Journal of the World Aquaculture Society.


1. Oliva-Teles, A, Lupatsch, I, Nengas, I. 2011. Nutrition and feeding of sparidae. In: Pavlidis, M, Mylonas, C (Eds). Biology of Sparids. Wiley Blackwell. ISBN: 978-1-4051-9772-4.



MASTER THESES

Cardoso, Inês. 2010. Substituição de farinha de peixe por farinhas vegetais em sargo legítimo, Diplodus sargus: efeitos na performance, digestibilidade e alterações da histologia do intestino. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: Aires Oliva Teles, Co-supervisor: Helena Peres.

Marinho, Gonçalo. 2010. Efeito do regime de alimentação – diurno, nocturno e misto – no crescimento, na utilização do alimento e na excreção azotada em juvenis de linguado Solea senegalensis. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: António Paulo Carvalho; co-supervisor: Helena Peres.


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- European Aquaculture Society Meeting “Aquaculture Europe 2010 - Seafarming Tomorrow”, 5-8 October 2010, Porto, Portugal. A Oliva Teles - Member of the Local Organizing Committee.


Within our group two projects are running in connection with the industry:

OPTISOLE – Investigação e desenvolvimento na produção de juvenis de linguado: incremento da resistência a patologias e do crescimento

- This is an industry based project submitted by A. Coelho e Castro, Lda. That aims solving specific problems of Senegalese sole rearing. Our group is responsible for establishing a growth model for S. sole juveniles based on temperature and diet quality. It is also aimed to establish the most adequate temperature for juvenile sole rearing and the best dietary protein to lipid ratio of the diets. Our group is also involved in evaluating the effect of larval rearing temperature on larval development and deformities.

Valorização do excedente de levedura da indústria cervejeira – potencial de utilização em dietas para os juvenis de linguado (Solea senegalensis)

- This project is founded by UNICER, within the framework of UP Pluridisciplinary projects, and aims at evaluating the potential use of brewers yeast in Senegalese sole juveniles diets. UNICER is a brewer company and brewers yeast is a by-product of this industry, therefore valorization of this sub-product is of particular interest for the company.


Seven out of eleven publications and a running PhD were done in collaboration with international researchers (Publications nr 2,3,4,5,9,10 and Other Publications nr 11).

Colleagues that collaborate in our publications during this year include: SJ Davies (University of Plymouth), A Krogdahl (Norwegian School of Veterinary Sciences), J Sanchez-Gurmaches (University of Barcelona), I Navarro (University of Barcelona), J Gutiérrez (University of Barcelona), I El Ghazali (University Cadi Ayyad), S Saqrane (University Cadi Ayyad), B Oudra (University Cadi Ayyad), Y Ouahid (Universidad Autonoma de Madrid), FF Del Campo (Universidad Autonoma de Madrid), I Lupatsch (Swansea University), Y Nengas (Hellenic Centre for Marine Research).



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Fisheries Biology and Hydroecology Research Group (FBHRG)



Fisheries Biology and Hydroecology Research Group (FBHRG)


José Pedro de Andrade e Silva Andrade

3. Location of group (Host institution)




- 5.821 EUR FCT Pluriannual funding.

- National funding:

74.846 EUR, FCT, PTDC/MAR/102348/2008, SEPIAMETA - On the metabolism of the European cuttlefish, Sepia officinalis (Linnaeus, 1758).


1. Objectives

Regarding cuttlefish culture, the 2010 scientific objectives were as follows:

- to determine the possibility of increasing cuttlefish fecundity and fertility under captivity through the use of increased bottom areas;

- to determine the nutritional and energy content of several different prey used in the culture of cuttlefish;

- to understand the usefulness of using anaesthetics as welfare promoters in the manipulation of cuttlefish juveniles and to determine the best anaesthetic agent.

As for non-scientific objectives and still regarding cuttlefish culture, these were to increase the amount of human resources of the group based on quality and to establish national and international cooperation on complementary fields of research to achieve the 5 year plan described in the 2009 report.


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Regarding seahorse ecology and culture, the scientific objectives for 2010 were as follows:

- to investigate the causes for the decline in seahorse population numbers registered in the Ria Formosa during the past 6-7 years;

- to determine the influence of pre-inductive photoperiod variations on long snout seahorse (Hippocampus guttulatus) broodstock response to an inductive photothermal program;

- to determine the effects of photoperiod and light intensity on growth, survival and activity of juvenile long snout seahorse (H. guttulatus);

- and, to evaluate the foraging, growth and survival of juvenile long snout seahorse (H. guttulatus) reared in different prey concentrations.


Regarding cuttlefish culture and scientific objectives, the main achievement was the identification of the best anesthesia agent to perform manipulation in European cuttlefish juveniles and what may be considered an acceptable stress. The results of this experiment will be presented on the forthcoming EUROCEPH 2011, where both researchers and EU will discuss the recently adopted EU Directive 2010/63/EU revising Directive 86/609/EEC on the protection of animals used for scientific purposes. The revised Directive brings all cephalopods (adults and immature forms) within the legislative framework currently covering research using vertebrates. A workshop session in this meeting is dedicated to a review of this legislation and the likely impact it will have on current and future research in this area and the practical implications for the welfare of these species.

The nutritional and energy content of several prey have been determined and will now be used to understand the growth, enzyme activity and metabolism of the species.

As for non-scientific objectives and still regarding cuttlefish culture, the group has 2 new technicians that were hired by SEPIAMETA and a new PhD candidate which will work on the inbreeding of cultured cuttlefish.

Regarding seahorse ecology, field data collected will help understand the human impact on the local seahorse communities and to determine which mitigation actions should be implemented. With respect to seahorse culture, it was possible to identify the optimal photothermal conditions to induce and implement out of season breeding programs, condition that will facilitate the completion of the future work plan. Beside that, we have optimized husbandry protocols regarding photoperiod, light intensity and prey concentrations that favour the best possible growth and survival of juvenile H. guttulatus in captivity.

In continuity with the previous year’s research guidelines, it was also possible to consolidate practical knowledge on juvenile seahorse culture with allowed us to establish a successful breeding program never attained before for this species.

As for non-scientific objectives a MsC thesis on seahorse breeding behavior was presented and several other small scale research plans by under graduated students were developed. During


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2010 we intensified the scientific cooperation with other international institutions, namely the Project Seahorse, the Zoological Society of London and the University of Guelph (Fish Nutrition Laboratory).

8d. Productivity


The FBHRG has published a total of 2 ISI-listed peer-reviewed articles, which resulted from international collaborations. The mean Impact Factor (based on ISI JCR 2009) for those 2 publications was 1.990. During this year, the FBHRG has published 0.5 publications (ISI) per FBHGR member with a PhD degree.

1. Hormiga, JA, Almansa, E, Sykes, AV, Torres, N. 2010. Model based optimization of feeding regimens in aquaculture: Application to the improvement of Octopus vulgaris paralarvae viability in captivity. Journal of Biotechnology 149: 209-214.

In press

2. Sykes, AV, Domingues, PM, Marquéz, L, Andrade, JP. (in press). The effects of tank colours on growth and survival of cuttlefish (Sepia officinalis, Linnaeus 1758) hatchlings and juveniles. Aquaculture Research.


3. Other publications national (Books, chapters or full papers published in conference proceedings


MASTER THESES

Fialho, Ana Isa Marques. 2010. Caracterização do comportamento reprodutivo do cavalo marinho de focinho comprido (Hippocampus guttulatus). Master thesis in Marine Biology, University of Algarve.





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Within the seahorse research program, the FBHG maintains a research partnership both with the Fish Nutrition Laboratory of the Department of Animal and Poultry Science of the University of Guelph, Canada leaded by Dr. Dominique P. Bureau and the Project Seahorse, an interdisciplinary and international organization committed to the conservation and sustainable use of the world’s coastal marine ecosystems. The on-going research activity with these institutions as rendered several scientific articles and an improvement of the scientific know how of the group members. Recently, a member of the FBHG, the PhD student Miguel Correia was invited to join Project Seahorse.

Regarding cuttlefish collaborative research, publication and graduate training, the FBHG maintains partnerships with the University of La Laguna (ERASMUS Program with the University of Algarve), the Spanish Institute of Oceanography in the Canary Islands and in Vigo, the CIFPA “Agua del Pino” in Huelva, and other laboratories of the ASSEMBLE project (227799 FP7). Despite a COST network regarding cephalopod aquaculture development was not approved for funding, the preparation of this proposal has extended the cooperation with other laboratories in the near future. Collaboration with the Department of Biochemistry and Molecular Biology of the Faculty of Medicine of the University of Malaga (Spain) has been developed within the frame of the analysis of inbreeding in consecutive generations of cuttlefish produced in captivity.



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Fisheries, Biodiversity and Conservation



Fisheries, Biodiversity and Conservation


Karim Erzini





- FCT pluriannual funding.

- National funding

23.630 EUR, FCT, PTDC/BIA-BDE/64539/2006.

- European funding

210.011 EUR, European Commission, LIFE NATURE, LIFE06 NAT/P/000192, BIOMARES.


1. Objectives

Fishing and fishing related activities are of considerable importance in the Algarve (southern Portugal), which is the Portuguese region with the highest percentage of the working population directly or indirectly involved in fisheries. The main objectives of the group are to contribute to the improved management and sustainable exploitation of the multi-species resources of the Algarve coastal waters. In recent years, the fisheries sector has been under considerable pressure, with a decline in landings of many of the most important species. Within this context, the main objectives of the group are to contribute to the improved management and sustainable exploitation of the multi-species resources of the Algarve coastal waters. Specific objectives include:


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- Studying the impact of commercial fishing activity and the implications for biodiversity: species and size selectivity of different gears, quantification of the by-catch and discards of different gears, and evaluation of ghost fishing;

- Studying the impact of recreational fishing activity;

- Finding ways to mitigate by-catch, discarding and to propose management measures and changes in legislation based on the above mentioned studies;

- Biology, population dynamics, and ecology of fish and invertebrates: growth and mortality parameters, maturity and reproduction, feeding ecology;

- Identification of essential fish habitat, by classification of the bottom, mapping of the biodiversity and distribution and abundance of key species;

- Long-term monitoring of key habitats and recruitment of fish and invertebrates;

- Evaluation of the effects of marine protected areas;

- Understanding the spatio-temporal dynamics of key commercial species by means of tagging and telemetry studies;

- Modeling and simulation studies for spatio-temporal dynamics, eco-trophic modeling, evaluating the effects of different management measures, and understanding the influence of environmental and fisheries factors.


2010 was the final year of the BIOMARES Life project, with several actions completed. Sixty two plots of seagrasses, with a total of 2,460 planting units (5,276 plant sods), were created and distributed along 7 ha of the target area. Monitoring of seagrass and fish was carried out using underwater methods and fishing gear (trammel nets and traps). Other important achievements were the organization of the European Seagrass Restoration Workshop, in September 2010, the launching of a seagrass conservation project “Adopt-a-seagrass-meadow”, and the successful use of acoustic telemetry to study habitat use and movements of fish and cuttlefish in the Arrábida marine park.

The study and mapping of the seabed from 0 to 30 meters depth between the mouth of the Arade River (Portimão) and Ponta da Piedade (Lagos) was finished. It was possible to identify a total of 567 species, consisting of 116 fish (17.5%), 461 (76.1%) benthic macroinvertebrates and 39 algae (6.4%) species, adding 48 new species to previous phases of this study (RENSUB I, II and III). In total, for all of these studies, approximately 1495 species (1349 animals + 146 algae/plants) were recorded, increasing what is already the largest inventory of marine species of the Algarve coast and one of the largest nationally. At this stage, the sampling effort allowed the discovery of three more new species recorded for the first time in Portugal/Europe (out of a total of 36 species). This project has contributed for the launching of two research projects on the measuring and determining the importance of marine invertebrates and the living microorganisms associated to them in the production of metabolites potentially useful for the pharmaceutical industry. The project has produced maps of habitats, densities, species richness,


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diversity, threatened species and an integrated map that would facilitate the decision making process related to the marine spatial planning.

8d. Productivity


1. Afonso, CML, Bonomolo, G, Monteiro, P, Bentes, L, Oliveira, F, Veiga, P, Rangel, MO, Sousa, I, Leite, L, Gonçalves, JMS. 2010. First record of Ocinebrina nicolai (Mollusca: Gastropoda: Muricidae: Ocenebrinae) in north-eastern Atlantic waters. Marine Biodiversity Records 3: 1-4.

2. Coelho, R, Bentes, L, Correia, C, Gonçalves, JMS, Lino, PG, Monteiro, P, Ribeiro, J, Erzini, K. 2010. Life history of the common pandora, Pagellus erythrinus (Linnaeus, 1758) (Actinopterygii: Sparidae) from southern Portugal. Brazilian Journal of Oceanography 58: 233-245.

3. Coelho, R, Rey, J, Gil de Sola, L, Fernandez-Carvalho, J, Erzini, K. 2010. Comparing NE Atlantic and Mediterranean populations of a deep water lanternshark, with comments on the efficiency of density dependant compensatory mechanisms. Marine Biology Research 6: 373-380.

4. Coelho, R. 2010. [Book Review]: Biology and Management of Dogfish Sharks, Edited by V.F. Gallucci, G.A. McFarlane & G.G. Bargmann. Marine Biology Research 6: 415-416.

5. Coelho, R., Erzini, K. 2010. Depth distribution of the velvet belly, Etmopterus spinax, in relation to growth and reproductive cycle: The case study of a deep water lantern shark with a wide ranging critical habitat. Marine Biology Research 6: 381-389.

6. Erzini, K, Gonçalves, JMS, Bentes, L, Lino, PG, Ribeiro, J, Moutopoulos, DK, Stergiou, KI. 2010. Competition between static gears of the small-scale fisheries in Algarve waters (southern Portugal). Mediterranean Marine Science 11(2): 225-243.

7. Korn, M, Green, AJ, Machado, M, García-de-Lomas, J, Cristo, M, Cancela da Fonseca, L, Frisch, D, Pérez-Bote, JL, Hundsdoerfer, AK. 2010. Phylogeny, molecular ecology and taxonomy of southern Iberian lineages of Triops mauritanicus (Crustacea: Notostraca). Organisms, Diversity & Evolution 10 (5): 409-440.

8. Marçalo, A, Marques, TA, Araújo, J, Pousão-Ferreira, P, Erzini, K, Stratoudakis, Y. 2010. Fishing simulation experiments for predicting the effects of purse-seine capture on sardine (Sardina pilchardus). ICES Journal of Marine Science 67: 334-344.

9. Mendonca, VM, Al Saady, S, Al Kiyumi, A, Erzini, K. 2010. Interactions between green turtles (Chelonia mydas) and foxes (Vulpes vulpes arabica, V. rueppellii sabaea, and V. cana) on turtle nesting grounds in the Northwestern Indian Ocean: Impacts of the fox community on the behavior of nesting sea turtles at the Ras Al Hadd Turtle Reserve, Oman. Zoological Studies 49: 437-452.

10. Monteiro, P, Bentes, L, Coelho, R, Correia, C, Erzini, K, Lino, PG, Ribeiro, J, Gonçalves, JMS. 2010. Age and growth, mortality and reproduction of the striped sea bream, Lithognathus


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mormyrus Linnaeus 1758, from the south coast of Portugal (Algarve). Marine Biology Research 6: 53-65.

11. Mourato, BL, Coelho, R, Amorim, AF, Carvalho, F, Hazin, FHV, Burgess, G. 2010. Size at maturity and length-weight relationships of the blurred lantern shark Etmopterus bigelowi (Squaliformes: Etmopteridae), caught off southeastern Brazil. Ciencias Marinas 36: 323-331.

12. Rey, J, Coelho, R, Lloris, D, Séret, B, Gil de Sola, L. 2010. Distribution pattern of Galeus atlanticus in the Alborán Sea (south western Mediterranean) and some sexual character comparison with Galeus melastomus. Marine Biology Research 6: 364-372.

13. Veiga, P, Ribeiro, J, Gonçalves, JMS, Erzini, K. 2010. Quantifying recreational shore angling catch and harvest in the south of Portugal (Northeast Atlantic): implications for conservation and integrated fisheries management. Journal of Fish Biology 76: 2216-2237.

14. Xavier JC, Cherel Y, Assis CA, Sendão J, Borges TC. 2010. The feeding ecology of Conger eels (Conger conger) in the North-east Atlantic. Journal of the Marine Biological Association of the United Kingdom 90: 493-501.


1. Delongueville, C, Scaillet, R, Afonso CML. 2010. Première signalisation de Nassarius sesarmus (Marrat, 1877) le long des côtes européennes de l’Atlantique Nord-Est (Algarve - sud du Portugal). NOVAPEX / Société Belge de Malacologie 11(4): 117-119.

2. Balasubramanian, A, Meenakumari, B, Boopendranath, MR, Pravin, P, Erzini, K. 2010. Mesh selectivity of drift gillnet for Caranx sexfasciatus and Caranx tille. Fishery Technology 47: 111-120.


Tavares, PC, Shapouri, M, Martins, C, Pereira, P, Falcão, M, Machado, M, Cancela da Fonseca, L. 2010. Variability in sources of organic matter influencing invertebrate community as revealed by pigment levels in mesoscale experiments. In: Martins, J, Tavares, A, Tavares, M, Ribeiro, L (Eds). Paula Tavares - Uma Ciência de Rosto Humano, CVRM - Instituto Superior Técnico, Lisboa, pp. 40-63.

Rangel, MO, Dentinho, TP, Araújo, G, Lopes, J, Gonçalves, JMS, Erzini, K. 2010 Análise custo viagem de roteiros subaquáticos (de apneia) na Praia da Marinha (Algarve). Revista Portuguesa de Estudos Regionais 22: 77-89.


MASTER THESES

Baptista, Miguel. 2010. Determination of age and growth of Galeus melastomus, Rafinesque, 1810, a deep water shark, using a modified cobalt nitrate technique European Master Thesis in


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Marine Biodiversity and Conservation, University of Algarve and University of Ghent. Supervisors: Rui Coelho, Karim Erzini.

Graça, Mariana João Dias. 2010. Caracterização da pesca grossa na ilha de Madeira. Master thesis in Marine Biology, University of Algarve. Supervisors: Karim Erzini, Mafalda Freitas (Marine Biological Station of Funchal).

Nhanca, Florentino João Lopes. 2010. Caracterização da pesca industrial na ZEE da Guiné-Bissau com vista ao seu desenvolvimento sustentável. Master thesis in Management and Nature Conservation, University of Algarve. Supervisors: Karim Erzini, Jorge MS Gonçalves.

Voguel, Camille. 2010. Geostatistical exploration of integrated VMS data from the Aran Ground Nephrops fishery with associated survey estimates: Establishing the relationship between fishing, biotic and abiotic variables. Erasmus Mundus Master in Biodiversity and Conservation, University of Algarve. Supervisor: Colm Lordan (Marine Institute, Galaway, Ireland), Co-supervisor: Margarida Castro.

PhD THESES

Bartilotti, Cátia Alexandra Vieira. 2010. Decapod crustacean larvae dynamics in the west continental coast of Portugal: the region adjacent to Aveiro coastal lagoon as model. PhD thesis in Marine Ecology, University of Algarve. Supervisor: Antonina dos Santos (IPIMAR), Co-supervisor: Margarida Castro.

Marçalo, Ana Luisa Barreto 2010. Avaliação de stress em sardinha (Sardina pilchardus) durante a pesca do cerco Universidade do Algarve Supervisors: Karim Erzini, Yorgos Stratoudakis (IPIMAR).



- Workshop sobre Biodiversidade Marinha na Arrábida, 22 May 2010, Sesimbra, Portugal. Alexandra Cunha – Organizer.

- XVI Iberian Symposium on Marine Biology, 6-10 September 2010, University of Alicante, Alicante, Spain. Karim Erzini, Alexandra Cunha - Members of the Scientific Committee.

- I European Seagrass Workshop, 30 September – 1 October, Portinho da Arrábida, Portugal.



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Teaching in the Masters Acuicultura y Pesca: Recursos Marinos Y Sostentabilidad, of the University of Cadiz: module on fishing gears and selectivity taught by K. Erzini.

Participation and coordination of the Erasmus Mundus Masters in Marine Biodiversity and Conservation (K. Erzini)

Participation in the BIOMARES Project of Dr. Chris Pickerell (Cornell University) and in the 1st European Seagrass Restoration Workshop (27 September - 2 October 2010) (A. Cunha)

Participation in the scientific cruise (RV METEOR) "Monitoring Atlantic seamounts life (Ampere)" in cooperation with the University of Hamburg. Atlantic ocean,16 November-23 December.

The group received a Tunisian fisheries scientist (Lofti ben Naceur) for a visit of one week, within the framework of the Luso-Tunisian collaborative project (Fishing gear characterisation and modernisation in Tunisia and Portugal), funded by the Ministry of Science and Technology / GRICES.


Project funded by ARH Algarve: Cartography and characterization of the marine communities off the National Underwater Ecological Reserve between the Arade river and Ponta da Piedade (RENSUB IV).

Gonçalves, JMS, Monteiro, P, Afonso, C, Oliveira, F, Rangel, MO, Machado, M, Veiga, P, Leite, L, Sousa, I, Bentes, L, Fonseca, L, Erzini, K. 2010. Cartografia e caracterização das biocenoses marinhas da Reserva Ecológica Nacional Submarina entre a foz do Rio Arade e a Ponta da Piedade. Relatório Final. ARH Algarve. CCMAR, Faro, 122 pp. + Anexos.


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Dina Cristina Fernandes Rodrigues da Costa Simes






- National funding

60.731 EUR, FCT, PTDC/SAL/ESA/101186/2008, BIOMARKGRP - Studies for the establishment of GRP as a biomarker for vascular calcification. Research Team: DC Simes (PI), CSB Viegas, ML Cancela, I Carvalho, PL Neves, A Ferreira Research Team:, A João, MJMV Gomes, ICS Pinto, APA Silva.


1. Objectives

Our team group as long expertise in the biochemical aspects of vitamin K-dependent proteins and particularly unique knowledge with respect of the biochemistry, molecular biology as well as biomedical aspects of a new Gla rich protein (GRP). The discovery of this new Gla protein, in which our team was involved and the submission of a patent with medical application have lead to the establishment of a new research line, based on collaborations that include Nephrology and Cardiology Department, Orthopedic and Trauma Department, Faro Hospital, Portugal; Department of Dermatology, Lisbon Central Hospital, Lisbon, Portugal

One important line of research of our group is diagnostics based on circulating GRP, which is a patented system to monitor cardiovascular disease via biochemical markers in blood. The biochemical aspects of this protein highly enriched in Gla residues turns this protein in one of the strongest inhibitors of pathological mineralization related diseases known to date. This circulating protein may reflect calcification processes and inhibition of those processes in the


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organism. Our main goal is to validate the ELISA–based assay that we have described as a valuable assay to study GRP as a marker of mineral associated pathologies (e.g. vascular calcification osteoarthritis and calcinosis associated diseases).

It is also our aim to establish GRP as a soft tissue calcification inhibitor, by studying GRP role and molecular mechanism underlying its function in vascular mineralization, contributing with new knowledge on the mechanisms leading to pathological calcifications, and with the identification of novel diagnostic and therapeutic strategies for the treatment of diseases related to ectopic ECM mineralization of vascular tissues.

Another goal of our team is the collaboration with other groups and research lines in the aquaculture field of research more related to the study of the relation of diet and supplementation on fish skeletal and muscle proteome as well the effects of factors that could influence flesh texture as accessed by post-mortem changes in fish muscle proteome.


We were able to confirm that GRP is a novel vitamin K-dependent protein present in serum widely expressed and accumulated in mammalian tissues, including soft and skeletal tissues, and associated with calcium crystals in situations of ectopic calcification, both in calcified arteries and skin calcinosis.

In this last year we were able to validate specific GRP antibodies for the use in human research and we showed that GRP is highly accumulated in the human skin and vascular system, and more importantly, is clearly associated with soft tissue calcification pathologies and ectopic calcifications in these tissues. We detected the presence of GRP in non-calcified vascular system. In calcified vascular system associated pathologies, GRP was found highly accumulated at sites of mineral deposition, co-localized with the mineral.

We have optimize the 2-DE Proteomics analysis procedures for the study of (1) human articular cartilage and the identification of osteoarthritis biomarkers (2) characterization of GRP present in human serum samples in healthy patients for comparison with dialysis patients with chronic kidney diseases stages (stages 1 to 5) (3) fractionation procedures for fish muscle proteomics (4) changes in the liver proteome of Senegalese sole in response to repeated handling stress (5) Proteomic analysis of nacre soluble matrix proteins from Crassostrea gigas.

8d. Productivity


1. Viegas, CS, Conceição, N, Fazenda, C, Simes, DC, Cancela, ML. 2010. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon (Acipenser naccarii). Journal of Applied Ichthyology 26: 214-218.

2. Alves, RN, Cordeiro, O, Silva, TS, Richard, N, de Vareilles, M, Marino, G, Di Marco, P, Rodrigues, PM, Conceição, LEC. 2010. Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics. Aquaculture 299 (1-4): 57-66.


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In press

3. Matos, E, Silva, TS, Tiago, T, Aureliano, M, Dinis, MT, Dias, J. (in press). Effect of harvesting stress and storage conditions on protein degradation in fillets of farmed gilthead seabream (Sparus aurata): A differential scanning calorimetry study. Food Chemistry, doi: 10.1016/j.foodchem.2010.11.017.


1. Silva, TS, Cordeiro, O, Jessen, F, Dias, J, Rodrigues, PM. 2010. On the reproducibility of a fractionation procedure for fish muscle proteomics. American Biotechnology Laboratory 28(4): 8-13.

3. Other publications national


MASTER THESES

Cavaco, Sofia. 2010. Isolamento e caracterização de proteínas contendo Gla da cartilagem e pele de mamífero. Master thesis in Molecular Biology and Microbiology, University of Algarve. Supervisor: Dina Costa Simes.

PhD THESES

Viegas, CSB. 2010. Identification and characterization of a new calcium binding protein from sturgeon, Gla rich protein or GRP: Contribution to unveil its function in vertebrates. PhD thesis in Biochemistry – Cellular and Molecular Biology, University of Algarve. Supervisor: Dina Costa Simes.


PATENTSCOPE publication number WO/2010/024704 - A gammacarboxyglutamate-rich protein, methods and assays for its detection, purification and quantification and uses thereof. Publication date: 04.03.2010.






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Geology and Paleoceanography



Geology and Paleoceanography


Fátima Abrantes (represented by João Coimbra)


CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental. Porto


The group which develops its activity at the Marine Geology Unit of LNEG was funded by several sources: Ministry of Economy and Innovation (MEI), FCT (national projects and Eurocores ESF), pluriannual funds through CIMAR and other funds for participation in meetings.

The funds financially executed at CIIMAR in 2010:



172.800 EUR, PTDC/CLI/70772/2006, INTER-TRACE, 2008-2011. PI: A Voelker.

175.163 EUR, PTDC/MAR/65197/2006, MELT KP5, 2008-2011. PI: P. Ferreira.

100.000 EUR, PTDC/AAC-AMB/108449/2008, MONA, 2010-2013. PI: I. Gil.

183.691 EUR, PTDC/MAR/102045/2008, CALIBERIA, 2010-2013. PI: E. Salgueiro.

199.976 EUR, PTDC/AAC-CLI/100157/2008, CLIMHOL, 2010-2013. PI: F. Naughton.

- European Regional Development Fund

106.280 EUR, INTERREG IV POCTEP, Natura Miño-Minho, 2009-2011. PI: C Antunes.

In addition, two national projects (SCARPS; HOLOCLIMA) and one ESF EuroMARC project (AMOCINT) –all with FCT funding– are executed at LNEG.


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1. Objectives

The general objectives of our group comprise: to execute the scientific projects that are a part of our main research program “INGMAR - Investigação em Geologia Marinha” with excellent quality; to submit and gain new projects; to publish our results in high quality journals; to actively contribute to the education of new technicians and marine geology researchers; and to disseminate the results obtained.

In scientific terms, the interaction between oceanic productivity, one of the main mechanisms controlling atmospheric pCO2, climate and ocean circulation, is one of the main study areas of the Paleoceanography/ Paleoclimatology and Environment group. As such we reconstructed productivity and climate conditions during particular intervals under different climate forcing conditions (e.g., CUPEX project) such as the Last Glacial maximum (LGM; 21.000 ±2000 yr ago) and the Holocene Climate Optimum (8000 ±1500 yr ago), or during older interglacial periods, i.e. periods with climate forcing similar to the current period (prior to the industrial revolution). For the older interglacials work focused on Marine Isotopic Stage (MIS) 5 (80.000 – 127.000 yr ago; AMOCINT project) and the glacial/ interglacial cycles within the period from MIS 9 to 16 (300.000 – 630.000 yr ago; PORTO & INTER-TRACE projects). The impact of interglacial climate variability during the Holocene, the current interglacial, on vegetation on both sides of the North Atlantic Ocean is being studied in the CLIMHOL project. Productivity changes under even warmer climate conditions, i.e. during the Pliocene (2.6 – 5.3 million yr ago), that might be similar to the future climate, are being reconstructed in the polar North Pacific (Bering Sea) based on material from IODP expedition 323.

Another major research topic is impacts of abrupt climate change, especially during the transition from a glacial to an interglacial (= deglaciation), on the ocean-climate system. The MONO project focuses on changes during the last deglaciation, while records from the AMOCINT and PORTO projects revealed abrupt climate changes during older periods.

No reconstruction of past climate change is, however, possible without the ground truthing of the parameters used, i.e. the calibration of proxies. Proxies can only be applied to past conditions if it can be shown on a regional scale or in comparison to instrumental data that the proxy is really reflecting the respective (hydrographic) conditions. We concentrate partly on plankton foraminifer based proxies in regard to stable isotope and trace element data (e.g., CALIBERIA project). To calibrate plankton foraminifer derived stable isotope data, analyzes are being done in the shells of alive or dead foraminifer shells collected with plankton tows throughout the eastern North Atlantic Ocean or a sediment trap off Vigo (Galicia) and in the surrounding seawater.

Another objective deals with the investigation of the origin of the different pollutants that reach the coastal regions and their relation with climate, vegetation, agriculture techniques and type of culture, that is, to evaluate the influence of human kind since 9.000 years B.P., through the analyses of persistent organic pollutants, heavy metals and biomarkers in the sediments of the Minho Estuary and the inner-shelf.

The SCARPS project of the coastal geology group aims to reconstruct the shoreline over the last 6000 yr and major phases of aeolian movements in the region spanning from the Troia


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Peninsula to Santo Andre, Costa da Caparica, Quiaios-Mira. Within the cooperation program with other Portuguese speaking countries, the coastal geology group collaborates with Mozambique and its national direction for geology “Direcção Nacional de Geologia de Moçambique” (Cooperation IPAD/LNEG/DNG) in the preparation of the Bilene Map.

Within the theme of natural resources, on-going research focuses on the Mid Atlantic Ridge (MAR) sulfides, the Fe-Mn nodules and shelf inerts. For the MAR study region, research so far concentrated on the various textural images of the seafloor that reflect differences in eruptive styles. Texture mapping TOBI side-scan sonar data and multibeam bathymetry data provides critical new views of the seafloor, views which can be used to gain a better understanding of the products of volcanism within the axial zone at the slow-spreading Mid-Atlantic Ridge and their spatial and temporal relationships.


The development of (new) proxy calibrations was continued during 2010 with the focus on the Tagus and Minho regions, the Portuguese margin and the North Pacific. C. Lopes et al. published a paper on the North Pacific diatom calibration. E. Salgueiro et al., on the other hand, are in the process of establishing regional calibrations for the trace element ratios of Mg/ Ca, Cd/ Ca and Ba/ Ca in Iberian margin plankton foraminifer shells. Plankton foraminifer and pteropod abundances in the plankton tow material of cruises PO383 and 384 in the Azores Front region were also determined.

Work continues on reconstructing oceanographic and climatic changes during interglacials. In their manuscript Voelker et al. showed that hydrographic during interglacial MIS 9e, 11c and 13a-c in the mid-latitude North Atlantic were overall not much different. The high-resolution Uk37’-Sea Surface Temperature record of core MD03-2699 off Portugal by Rodrigues et al. (in press) confirms this observation and highlights the long-term temperature stability during MIS 13 (535.000 – 475.000 yr ago). Productivity and input of terrestrial biomarkers, on the other hand, were higher during MIS 13 than the younger interglacial MIS 11c and 9e. Proxy records for productivity and North Atlantic overturning strength from core MD01-2446, retrieved from the Tore Seamount, indicate that open ocean productivity increased during the colder phases of MIS 5 and during glacial MIS 6 and 4 suggesting higher nutrient availability during times when the overturning circulation was reduced.

A high-resolution diatom study in deep-sea records (cores OCE326GGC26 and OCE326GGC14) from the Laurentian fan off Nova Scotia (Canada), covering the last 19.000 years, revealed high variability in the sea ice cover during the last deglaciation.

The diatom study in Pliocene sediments from sites drilled during IODP Exp. 323 lead to adjustment in the regional biostratigraphy and to the finding of species that were previously only known from modern sediments. A detailed study on the diatom-rich laminated sections with an age of 3.03 Ma is underway to ascertain if these laminations (or layers) were produced by changes in diatom productivity and if we can determine seasonal succession.

The study on the interaction between hydrographic conditions and vegetation during the Holocene by F. Naughton concentrates currently on core KNR178JPC32 off the Cape Hatteras (USA) for which a low-resolution pollen record was established. F. Naughton, furthermore,


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compiled a pollen atlas consisting of three volumes for North American, an essential tool for her work.

The multi-proxy study from the drill site in the Tagus estuary near Vila Franca de Xira revealed changes in terrestrial and marine input to this location during the last 14.000 yr (Master thesis of C. Santos). Although discontinuous, the SST record shows a decreasing trend from Early to Late Holocene. Important terrigenous input is recorded during the Younger Dryas/Holocene transition when sea level rise allowed sediment accumulation inside the estuary.

For the Natura Miño – Minho project a suite of proxy data (e.g., element composition; grain size; diatom abundance; biomarkers) was generated for the surface samples and the DIVA 09 core collected on the shelf. For the DIVA 09 core additional available proxy records include XRF and planktonic and benthic foraminifer stable isotope records. The age model for this core was established based on 210Pb and 14C measurements and the core covers the last 2000 years. Within the estuary, field work during 2010 lead to the selection of sites to be drilled and finally two drill cores. One of the drill cores yielded a 14C age of about 6700 yr B.P. For the estuary cores, XRF and diatom abundance data already exists.

For the SCARPS project several km of geophysical (GPR) and topographic surveys (beach profiles) were completed and cores for detailed studies retrieved. Furthermore, samples were collected for OSL analyses. The collaboration with Mozambique and its national direction for geology led to the completion of the Bazaruto Map (1:25,000). The study on the evolution of the shelves of the Azores islands resulted in the publication by R. Quartau et al. about Faial.

The MELT KP5 project suffered a major setback when the planned study of the Menez Gwen segment of the MAR could not be done during the Coralfish cruise in August 2010. Thus work currently focuses on the interpretation of existing TOBI side-scan sonar and multibeam bathymetry data and on the chemical analyses of MAR basalt samples collected during previous cruises.

8d. Productivity


1. Cermeno, P, de Vargas, C, Abrantes, F, Falkowski, PG. 2010. Phytoplankton biogeography and community stability in the Ocean. Plos One 5(3): e10037.

2. Fletcher, WJ, Sanchez-Goni, MF, Allen, JRM, Cheddadi, R, Combourieu-Nebout, N, Huntley, B, Lawson, I, Londeix, L, Magri, D, Margari, V, Mueller, UC, Naughton, F, Novenko, E, Roucoux, K, Tzedakis, PC. 2010. Millennial-scale variability during the last glacial in vegetation records from Europe. Quaternary Science Reviews 29: 2839-2864.

3. Flores, J-A, Colmenero-Hidalgo, E, Mejia-Molina, AE, Baumann, K-H, Henderiks, J, Larsson, K, Prabhu, CN, Sierro, FJ, Rodrigues, T. 2010. Distribution of large Emiliania huxleyi specimens in the Central and Northeast Atlantic during the last 25,000 years and its utility to monitor the onset of warm conditions. Marine Micropaleontology 76: 53-66.


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4. Gil, IM, Keigwin, LD, Abrantes, FG. 2010. Comparison of diatom records of the Heinrich event 1 in the Western North Atlantic. IOP Conference Series: Earth and Environmental Science 9: 012008.

5. Kjellström, E, Brandefelt, J, Naeslund, JO, Smith, B, Strandberg, G, Voelker, AHL, Wohlfarth, B. 2010. Simulated climate conditions in Europe during the Marine Isotope Stage 3 stadial. Boreas 39: 436-456.

6. Lopes, C, Abrantes, F, Mix, AC. 2010. Environmental controls of diatoms species in North-east Pacific sediments. Palaeogeography, Palaeoclimatology, Palaeoecology 297: 188-200.

7. Mil-Homens, M, Costa, A, Lebreiro, S, Canário, J, Lopes, C, Mouro, F, Mateus, M, Stigter, H, Richter, T, Branco, V, Trancoso, M, Melo, Z, Boer, W. 2010. Temporal clustering of metals in a short sediment core of the Cascais Canyon (Portuguese Margin). Scientia Marina 74(S1): 89-98.

8. Rodrigues, T, Grimalt, JO, Abrantes, F, Naughton, F, Flores, J-A. 2010. The last glacial-interglacial transition (LGIT) in the western mid-latitudes of the North Atlantic: Abrupt sea surface temperature change and sea level implications. Quaternary Science Reviews 29: 1853-1862.

9. Rogerson, M, Colmenero-Hidalgo, E, Levine, RC, Rohling, EJ, Voelker, AHL, Bigg, GR, Schönfeld, J, Cacho, I, Sierro, FJ, Loewemark, L, Reguera, MI, de Abreu, L, Garrick, K. 2010. Enhanced Mediterranean-Atlantic exchange during Atlantic freshening phases. Geochemistry, Geophysics, Geosystems 11(8): Q08013.

10. Salgueiro, E, Voelker, AHL, de Abreu, L, Abrantes, F, Meggers, H, Wefer, G. 2010. Temperature and Productivity Changes off the Western Iberian Margin during the last 150 ky, Quaternary Science Reviews 29: 680-695.

11. Voelker, AHL, Rodrigues, T, Billups, K, Oppo, D, McManus, J, Stein, R, Hefter, J. Grimalt, JO. 2010. Variations in mid-latitude North Atlantic surface water properties during the mid-Brunhes (MIS 9-14) and their implications for the thermohaline circulation. Climate of the Past 6(4): 531-552.

In press

12. Rodrigues, T, Voelker, AHL, Grimalt, JO, Abrantes, F, Naughton, F. (in press), Iberian Margin Sea Surface Temperature during MIS 15 to 9 (580-300 ka): Glacial suborbital variability vs. interglacial stability, Paleoceanography, doi: 10.1029/2010PA001927.


1. Abrantes, F, Voelker, A, Sierro, FJ, Naughton, F, Rodrigues, T, Cacho, I, Ariztegui, D, Brayshaw, D, Sicre, M-A, Batista, L., in press. Paleoclimate Variability in the Mediterranean Region. In: Lionello, P (Ed). Mediterranean Climate: from Past to the Future.

2. Naughton, F, Drago, T, Sanchez-Goñi, MF, Freitas, MC, Andrade, C. (in press). Climate variability in the north-western Iberian Peninsula during the last deglaciation. In: Pedro Duarte,


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M da Silva, Santana-Casiano, M (Eds). Oceans and the Atmospheric Carbon Content. Springer editions.

3. Voelker, AHL, de Abreu, L. (in press). A Review of abrupt climate change events in the Northeastern Atlantic Ocean (Iberian Margin): Latitudinal, longitudinal and vertical gradients. In: Rashid, H, Polyak, L, Mosley-Thompson, E. (Eds). Understanding the Causes, Mechanisms and Extent of the Abrupt Climate Change. AGU Geophysical Monograph.

4. Voelker, AHL, Lebreiro, S. 2010. Millennial-scale changes in deep water properties at the middepth western Iberian margin linked to Mediterranean Outflow Water activity. Extended abstract, Deep Water Processes and Products Congress, Baiona, Spain. Geo-Temas 11, Spanish Geological Society (Sociedad Geológica de España).


1. Nave, S, Lebreiro, S, Kissel, C, Guihou, A, Figueiredo, MO, Silva, TP, Michel, E, Cortijo, E, Labeyrie, L, Voelker, A. 2010. Variações da produtividade oceânica durante o último Interglaciar e a sua relação com a Circulação Thermohalina (Open ocean productivity changes during the Last Interglacial and its relation to the Atlantic Meridional Overturning Circulation). Actas do VIII Congresso Nacional de Geologia, Braga, Portugal. e-terra, vol 12, nº 13, Julho de 2010, http://e-terra.geopor.pt.


MASTER THESES

Santos, Célia. 2010. Reconstrução das condições paleoambientais e paleoclimáticas no estuário do Rio Tejo durante o Holocénico (~12 000 anos). Master thesis in Sea Sciences – Marine Resources, ICBAS, University of Porto. Supervisor: F Abrantes.



- Steering Committee for the ESF programme on “Workshops on Marine Research Drilling

(Magellan Workshop Series)”, February 2010, Ponta Delgada, Azores, Portugal. F Abrantes - Organizer.

- Congress on Deep Water Processes and Products, Baiona (Spain), 16-18 June 2010. F. Abrantes - Member of the Scientific Committee.

- II Seminário Ibérico IGBP – International Geosphere Biosphere Programme “Mudança ambiental global na Península Ibérica – Uma visão integrada”, November 2010, Lisbon, Portugal. F Abrantes – Member of the Scientific Committee.


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- Congress on Deep Water Processes and Products, 16-18 June 2010, Baiona, Spain. A Voelker – Member of the Executive Committee.

- AGU Fall Meeting session on “Interglacial Climate Variability”, December 2010, San Francisco, USA. A Voelker - Initiator and Convenor.



Research stays and advanced training:

- University of Vigo, Spain; M Rufino as Visiting scientist; January 2010.

- Malmo, Sweden; 53rd Multibeam Sonar Training Course - Ocean Mapping Group (University of New Brunswick) and Center for Coastal & Ocean Mapping (University of New Hampshire); March 2010; R Quartau.

- University of Bremen, Germany; I Gil as Visiting Scientist; April-June 2010.

- IODP Summer School “Ocean and climate changes in polar and subpolar environments” at the Université du Québec à Montreal (UQAM) and the Université du Québec à Rimouski, Canada; June-July 2010; C Ventura.

- ECORD Summer School “Dynamics of Past Climate Change” at MARUM, University Bremen, Germany; September 2010; A Rebotim, C Santos, L Matos and S Mateus.

- CSIC Vigo, Spain; week-long visits by E Salgueiro in July and September 2010 and by A Rebotim in October-November 2010.

Oceanographic campaigns

- SO206 Cruise: “Investigations on active dewatering sites at the convergent margin off Costa Rica (pre-site survey for IODP proposal 633)”; June 2010; Participant: M Nuzzo.

- KNR197-10 cruise on R/V KNORR from Ponta Delgada, Açores, to Woods Hole, USA; July 2010; Participant: I Gil.

- “Acção Integrada Luso Espanhola” field work; Lagoa de Sto Andre, Portugal; Participant: S Costas.

Meeting Organization

- A Voelker: Executive committee member for the Congress on Deep Water Processes and Products in Baiona, Spain, June 2010.


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- F Abrantes: Executive committee member for II Seminário Ibérico IGBP “Mudança ambiental global na Península Ibérica – Uma visão integrada”, Lisbon, Portugal; November 2010.

- A Voelker: AGU Fall Meeting session on “Interglacial Climate Variability”, San Francisco, USA; December 2010.

National delegation

- ECORD Science Support and Advisory Committee (ESSAC) of IODP; F Abrantes, A Voelker.

- IMAGES project Scientific Committee (SciCom); A Voelker.

- PAGES project SciCom; F Abrantes.

- ESF MedCLIVAR project SciCom; F Abrantes.

- COST Action ES0907: The INTIMATE Project: INTegrating Ice core, MArine and TErrestrial records (60,000 to 8000 years ago); A Voelker

Contribution to international projects

- Results from CALIBERIA and INGMAR projects to GEOTRACES (SCOR program).

- Results from PORTO, INTER-TRACE and AMOCINT projects to PAGES PIGs (Past Interglacials) working group.



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Hydrobiology



Hydrobiology


Adriano Agostinho Donas Bôto Bordalo e Sá





- National funding

30.000 EUR, FCT ERA-AMPERA/0003/2007, OILDEBEACH.

23.000 EUR, Annual research funding ICBAS-UP.

35.000 EUR, QREN-2009/003442, ECOPISCIS, 2009-2011.

130.000 EUR, FCT, PTDC/AAC-CLI/103539/2008.

123.706 EUR, FCT, PTDC/MAR/098914/2008.

120.000 EUR, FCT, PTDC/MAR/099140/2008.

INAG, POVT-03-0133-FCOES-00017, Avaliação do estado ecológico das massas de água costeiras e de transição e do potencial ecológico das massas de água fortemente modificadas. 2009-2011.

Suldouro, Portugal - Avaliação do potencial de eutrofização do Rio Inha na envolvente da ex- lixeira de Canedo, 2009-2010.

AHR Norte, River Douro estuary management plan. Development of the model for tenders call, 2009-2010.


Aida, Spain - Seasonal variability of water quality in the new water network of Bolama, Bijagos archipelago; Assessment of water quality in wells in the Buba area, Guinea-Bissau.


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Spanish Red Cross - Assessment of water quality in wells and boreholes in the Gabu, Bafatá and Bubaque areas, Guinea-Bissau.

- FCT Scholarships

5.500 EUR, SFRH/BD/47631/2008, SFRH/BD/46146/2008.

- Others

4.000 EUR, Univ Porto, Microbial Life in Extreme Antarctica Environments.

500 EUR, Univ Porto, Escola de Ciências da Vida e da Saúde. Summer course.


1. Objectives

The activities within the lab, in what CIIMAR is concerned, are spread over three main areas: (i) research; (ii) consulting; and (iii) outreach. The lab aims to contribute to the advance of science from a multidisciplinary point of view, dealing from water quality monitoring to modelling, from early life stages of fish to denitrification and global warming and environmental and human health. Research is carried out in temperate (Portugal), tropical (Thailand - SE Asia, Guinea- Bissau - W Africa, S. Tomé and Principe - W Africa) and extreme environments (Antarctica).

The main research areas are:

- Monitoring estuarine water quality in terms of physical (conductivity, salinity, temperature, turbidity), chemical (dissolved oxygen, inorganic carbon, pH, nitrate, nitrite, ammonium, phosphate, silicate) and biological (bacteria, viruses, fecal indicators, primary production) parameters;

- Estuarine primary productivity;

- Nitrogen and sulfur cycles; processes, interactions and microbial communities implicated;

- Effects of N and S cycles disturbances on cumulative global warming potential;

- Ecology of microbial communities in the extreme cold environments of Antarctic ice-free Dry Valeys, with a focus on the main N cycle microbial contributors;

- Anthropogenic impact in estuarine communities;

- Phytoremediation and bioremediation, highlighting the role of the both plants and microorganisms in the removal of metals and hydrocarbons from estuarine and coastal sediments;


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- Estuarine fish assemblages, with special emphasis to the estuarine nursery role for early life stages of fishes;

- Implementation of the Water Framework Directive (WFD);

- Ecological modelling;

- Tropical water quality;

- Ecology of cholera;

- Water management and public health.


Monitoring estuarine water quality

- Research in the River Douro estuary involving monthly water column (since 1985) key environmental data.

- Estuarine primary productivityResearch in the River Douro estuary has been performed in the water column, intertidal sediments and hard surfaces.

Nitrogen cycle and global warming

- Research has been carried out in the water, sediments and hard surfaces in several estuaries in Portugal as well as in Thailand. Nitrogen fixation, nitrification, denitrification, annamox and nitrous oxide production, a powerful green-house gas were evaluated. In addition, the structure of the microbial populations involved in nitrification and denitrification and anammox are also studied.

Nitrogen and Sulfur Cycle interactions

- Discovery of the occurrence of a novel inhibitory interaction between organic sulfur degradation compounds and the last step of denitrification (i.e. reduction of N2O and N2) in estuarine sediments and in a bacterial pure culture (Silicibacter pomeroyi). The inhibition of the last step of denitrification limits nitrogen loss through N2 and enhance nitrogen loss via N2O, a greenhouse gas. These findings represent a new perspective concerning the interaction and controls between nitrogen and sulfur cycles in the marine environment and promote a new line of research that is being currently investigated.

Ecology of Microbial communities in Antarctic Soils

- Research on bacteria cyanobacteria and ammonia oxidizers biodiversity and response to environmental constraints has been carried out in the ice-free regions of Transantarctic Mountains. A multidisciplinary approach to understand how geological history,


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geographic gradients, and soil geochemical variables drive spatial differences in diversity and distribution of the microbial communities was used.

Anthropogenic impact on estuarine communities

- Research performed in estuaries in order to understand the effect of heavy metals and other pollutants on macrobenthic and microbial communities and on the denitrification process with a main focus on N2O emissions.

Phytoremediation and bioremediation

- The role of the interaction between plants and microorganisms in the removal of heavy metals and organic pollutants from sediments has been studied in several estuaries in Portugal (Douro, Cávado and Lima). Additionally, the feasibility of bioremediation as a clean-up tool of subsurface oil has been evaluated, by studying microbial communities, and their hydrocarbon degradation potential, in beaches that still contaminated several years after the sinking of the Prestige oil tanker (N.W. Spain).

Estuarine fish assemblages

- Understanding of fish dynamics, focusing on the potential nursery role of estuaries and ultimately assess the carrying capacity of these ecosystems for fish species, some with high economical important such as sardines and flatfishes. Deriving and testing of an ecological indicator of the fish estuarine nursery function, to evaluate the ecological status of estuarine ecosystems, towards the implementation of the Water Framework Directive.

Implementation of the WFD

- Research conducted in several northern Portuguese estuaries in order to assess the ecological status of transitional waters and develop tools towards the implementation of the Water Framework Directive.

Ecological modeling

- By means of an object-oriented model several key parameters, including primary production, have been modelled in the River Douro estuary.

Tropical water quality, cholera and public health

- Research in Thailand, Guinea-Bissau and S. Tomé & Principe. In Thailand the water quality of the Bangpakong River watershed and subtidal sediments has been carried out. In Guinea-Bissau, water quality and its relation to disease including cholera has been evaluated and sustainable measures do increase water quality have been designed. In S. Tomé & Principe the water quality in the island of S. Tomé was evaluated for the first time.


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Outreach

- Several activities fostering the dialogue between scientists and society, to improve the public knowledge and perception of science and to increase the interest of the young in science, namely through participation in the summer courses of University of Porto (Universidade Junior) and collaboration with Ciência Viva (Projecto Oceanos, Biodiversidade e Saúde Humana) will be implemented. Moreover, scientific sound measures to reduce the risk of using contaminated water to be implemented in a sustainable way in Wets Africa will be developed.

8d. Productivity


1. Teixeira, C, Magalhães, C, Boaventura, RAR, Bordalo, AA. 2010. Potential rates and environmental controls of denitrification and nitrous oxide production in temperate urbanized estuary. Marine and Environmental Research 70: 336-342.

2. Ramos, S, Ré, P, Bordalo, AA. 2010. Recruitment of flatfish species to an estuarine nursery habitat (Lima estuary, NW Iberian Peninsula). Journal of Sea Research 64: 473-486.

3. Azevedo, IA, Duarte, PM, Bordalo, AA. 2010. Temporal and Spatial variability of phytoplankton photosynthetic characteristics in a southern European estuary (Douro, Portugal). Marine Ecology Progress Series 412: 29-44.

4. Mucha, APC, Almeida, CMR, Bordalo, AA, Vasconcelos, MTSD. 2010. LMWOA exudation by salt marsh plants: natural variation and response to Cu contamination. Estuarine, Coastal and Shelf Science 88: 63-70.

5. Azevedo, IA, Bordalo, AA, Duarte, PM. 2010. Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersal in the Douro estuary (Portugal). Water Research 44: 3133-3146.

6. Carvalho, PN, Basto, MCP, Silva, MFGM, Machado, A, Bordalo, AA, Vasconcelos, MTSD. 2010. Ability of salt marsh plants for TBT remediation in sediments. Environmental Science Pollution Research 17: 1279-1286.

In press

7. Carvalho, L, Cortes, R, Bordalo, AA (in press). Evaluation of the ecological status of an impaired watershed by using a multi-index approach. Environmental Monitoring and Assessment, doi: 10.1007/s10661-010-1473-9.

8. Ribeiro, H, Mucha, AP, Almeida, MCR, Bordalo, AA (in press). Hydrocarbon degradation potential of salt marsh plant microorganisms associations. Biodegradation. doi: 10.1007/s10532-010-9446-9.


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9. Mucha, AP, Almeida, CMR, Magalhães, CM, Vasconcelos, MTSD, Bordalo, A. (in press). Salt marsh plant-microorganism interaction in the presence of mixed contamination. International Biodeterioration & Biodegradation, doi: 10.1016/j.ibiod.2010.12.005.




MASTER THESES

Amorim, Eva. 2010. Bacterial indicators of bathing water quality in Douro estuary and Porto coastal front. Master thesis in Sciences of the Sea-Sea Resources, ICBAS, University of Porto. Supervisor: Adriano A Bordalo e Sá, Co-supervisor: Isabel Azevedo.




Aida, Spain - Seasonal variability of water quality in the new water network of Bolama, Bijagos archipelago, Guinea-Bissau.

Aida, Spain - Assessment of water quality in wells in the Buba area, Guinea-Bissau.

Spanish Red Cross, Spain - Assessment of water quality in wells and boreholes in the Gabu, Bafatá and Bubaque areas, Guinea-Bissau.Suldouro, Valorização e Tratamento de Resíduos Sólidos Urbanos, SA (Portugal) - Avaliação do potencial de eutrofização do Rio Inha na envolvente da ex-lixeira de Canedo.

Northern River Basin Administration (ARH-Portugal) - River Douro estuary management plan. Development of the model for tenders call.


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United Kingdom

- IECS, U. Hull: Prof. M. Elliott. Derive and test a quantitative indicator of ecosystem functioning and health for use in management plans and the future policies for the estuarine management.

Spain

- GEOMA, University of Vigo. Prof. Ana Bernabeu. Buried oil in the intertidal beach zone: coupling between beach morphodynamic, natural degradation, forcing mechanisms and biological activity.

France

- GLADYS, University of Montpellier 2. Prof. Frédéric Bouchette. Buried oil in the intertidal beach zone: coupling between beach morphodynamic, natural degradation, forcing mechanisms and biological activity.

United States of America

- Dept of Marine Science, UGA: Prof. W Wiebe, Prof. J Hollibaugh, Prof. S Joye. Structure and function of microbial communities; biogeochemical cycling of nutrients.

- RSMAS, University of Miami: Prof. R Cowen and Prof. C Paris, Fisheries oceanography and larval ecology.

- Dauphin Island Sea Lab, University of South Alabama. Prof. R Kiene. Cycling of organic sulfur in aquatic systems.

- Dep. of Microbiology, University of Tennessee. Prof. A Buchan. Ecology of microbial communities that mediate biogeochemical processes of organic sulfur cycling.

- University of Maryland. Prof. Rita Colwell. Cholera dynamics in aquatic systems.

New Zealand

- University of Waikato. Prof. C Cary, Prof. I R McDonald. Microbial diversity and environmental constrains along latitudinal gradient of Antarctic terrestrial environments.

Brasil

- LIZI, Universidade Federal do Rio de Janeiro: Prof. Ana Bonecker. Ichthyoplankton biology and ecology in tropical and temperate estuaries.

Guinea-Bissau


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- Variability of well/borehole water quality dry/wet seasons, outbreak of intestinal diseases and cholera. AIDA (NGO Ayuda, Intercambio y Desarrollo) and Spanish Red Cross.

Thailand

- University of Burapha: Prof. Kashane Chalermwat. Microbial dynamics and activity on Bangpakong estuary.


QREN-2009/003442: ECOPISCIS. 2009-2011.

POVT-03-0133-FCOES-00017, INAG, EEMA - Avaliação do Estado Ecológico das Massas de Água Costeiras e de Transição e do Potencial Ecológico das Massas de Água Fortemente Modificadas. 2009-2011.

Suldouro, Valorização e Tratamento de Resíduos Sólidos Urbanos, SA - Avaliação do potencial de eutrofização do Rio Inha na envolvente da ex-lixeira de Canedo. 2009-2010.

Seasonal variability of water quality in the new water network of Bolama, Bijagos archipelago, Guinea-Bissau. Aida, Spain. 2008-2011.

Assessment of water quality in wells in the Buba area, Guinea-Bissau. Aida, Spain. 2010-2011.

Assessment of water quality in wells and boreholes in the Gabu, Bafatá and Bubaque areas, Guinea-Bissau. Spanish Red Cross. 2009-2010.


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Marine Biotechnologies (MarBiotech)



Marine Biotechnologies (MarBiotech)


João Carlos Serafim Varela




Funding spent in 2010:



61.328 EUR, PTDC/MAR/103957/2008, SEABIOMED - Marine photosynthetic organisms of the Algarve coast with biomedical applications.

20.179 EUR, PTDC/BIA-MIC/101036/2008, CYANO - Regulation of iron homeostasis in cyanobacteria.


1. Objectives

Up until recently, the main research field of the MarBiotech (Marine Biotechnology) research group was focused on the elucidation of the molecular basis of the elevated carotenoid accumulation in the microalga Dunaliella salina. However, as the marine biotechnology scientific field at large became quite dynamic, the Principal Investigator decided to widen the scientific objectives of the group, extending the MarBiotech research interests over to three emerging research topics: 1) algae-derived biofuels; 2) marine bioactive compounds; and 3) iron homeostasis in cyanobacteria. Line Research 2) is essential for the implementation of a bio-refinery strategy for the production of High Valuable Products (HVPs). HVPs will be used to defray production costs of the biomass and enable entrepreneurial partners to produce biofuels (mainly biodiesel and bio-jetfuel) at competitive prices with petrodiesel.


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At the moment, line research 1) has undergone a slight strategic shift. Instead of focusing on microalgae-derived biofuels alone, MarBiotech has extended the search for ways of producing biofuels by anaerobic digestion of seaweeds in collaboration with Dr. Alistair Sutherland, a group leader at the Department of Biological and Biomedical Sciences at the Glasgow Caledonian University, UK. This common effort has culminated with the submission of a Marie Curie Fellowship by Dr. Sutherland to come over to CCMAR and develop part of this research at our facilities.

The study of iron homeostasis is a recent research line due to a collaborative effort of MarBiotech with Dr. Matthias Futschik’s lab at CBME (University Algarve) and Prof. Wolfgang Hess’s CyanoLab (University Freiburg, Germany) using a systems biology approach to understanding how iron availability is regulated in ecologically relevant cyanobacteria. This study will be in the near future extended to understand how iron homeostasis and photosynthesis regulatory networks interact. The use of systems biology will be a powerful, innovative way of understanding how primary productivity in the marine environment is modulated by iron availability at the genetic and molecular levels.


In 2010, in collaboration with Atlantik Fish, S.A., a Portuguese aquaculture company established on the East coast of Algarve, MarBiotech initiated a research line in order to find autochthonous microalgae strains with suitable lipid profiles for biofuel production. The final goal is to obtain microalgae able to grow in low cost photobioreactors in the Atlantik Fish facilities with high lipid productivity. Due to this effort, we were able to isolate several strains, one of which a Tetraselmis sp. strain (AF3G) that showed a very promising lipid profile (high lipid content with low amounts of polyunsaturated fatty acids) suitable for the production of biodiesel (Pereira et al. 2010; Sobral, 2010). Interestingly, in the implementation of the SEABIOMED project, this microalgal strain showed very high levels of radical scavenging, suggesting that its biomass will be a good source of antioxidants as well (Custódio, 2010). This is in agreement with the biorefinery concept in which biomass can be used for different ends, maximizing its value.

In collaboration with Necton, S.A. and Atlantik Fish, S.A the MarBiotech research group screened for bioactive compounds in 36 marine photosynthetic organisms (SEABIOMED project), namely 24 species of macroalgae (12 phaeophytes, 6 rhodophytes, 6 chlorophytes), 2 seagrasses (Zostera noltii and Cymodocea nodosa) and 10 microalgal strains (7 chlorophytes, 1 eustigmatophyte, 1 cryptophyte and 1 heterokontophyte). From these samples, more than 100 different extracts were generated by conventional solvent extraction, of which about 40% showed significant antioxidant activity and 30-40% displayed iron and copper chelating activity. From the 39 extracts screened for acetylcholinesterase (AChE) inhibitory activity, 46% exhibited moderate to high activity, and about 60% of the fractions tested seem to have antitumoral activity. Concerning the latter bioactivity, the most promising candidates are Phaeophyte brown algae, which showed IC50 values lower than 50 µg of crude extract for tumoural cells. Conversely, in non-tumoural cells the extracts did not reduce cell viability significantly at the concentrations that were toxic to tumour cells. In addition, the MarBiotech group carried out a one-day field survey in order to assess the halophyte biodiversity present in a limited area of the Algarve (Praia de Faro and adjacent salt marshes) and in an aquaculture facility (Atlantik Fish). This one-day prospection resulted in the collection of 25 species, belonging to 23 genera. From the species collected, 85% were still unexplored from the biomedical point of view. Five


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different extracts from 5 species were tested for cytoxicity against 2 human cancer cells and a non-cancerous human cell line. About 56% of these extracts significantly reduced the viability of both cancer cell lines after a 72h treatment at a concentration of 125 µg/ml. Moreover, 2 extracts of a Juncus spp showed significantly lower toxicity against non-cancerous cells. We also evaluated methanol crude extracts from 18 species concerning neuroprotection: 11% had moderate cholinesterase inhibitory (ChEI) activity, and 28% exhibited potent ChEI coupled with copper and iron chelation.

Similarly, in the CYANO project, and on schedule, we have built a first draft of the regulatory network of genes involved in ion homeostasis in enterobacteria (E. coli) as well as in cyanobacteria (Synechocystis sp.; Prieto, 2010; Prieto et al., 2010). An interesting result coming out of this study is that non-coding RNAs (ncRNAs) seem to play an important role in the regulation of ion homeostasis in bacteria, as they regulate the central node of the iron regulatory network (Fur gene product). This work was carried out in collaboration with Wolfgang Hess and Claudia Steglich, using data from three different independent datasets (Kegg expression, GEO and ArrayExpress databases). A manuscript describing these results is in preparation.

8d. Productivity



3. Other publications national








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Marine Community Ecology and Evolution (LMCEE)



Marine Community Ecology and Evolution


Elsa Maria Branco Froufe Andrade






68.088 EUR, PTCD/MAR/109954/2009, RAP - Responses to Anthropogenic Perturbations: climatic and nutrient effects on rock pool assemblages. Starting in the beginning of 2011. 118.743 EUR, PTDC/MAR/109954/2009, OCEAN KELP - Effects of ocean climate on the macroecology and resilience to disturbances of kelp beds. Starting in the beginning of 2011.

- European funding

1.0693.694 EUR, EEA Financial Mechanism, Managing effects of global climate on estuarine biodiversity and productivity. Ending in 30 April 2011.


1. Objectives

The scientific strategies of the LMCEE will integrate cross-discipline approaches related to the different levels of organization of marine and estuarine biodiversity: from genome over organism, to populations, communities and ecosystems across biogeographical regions. Such an approach is key to gain a complete understanding of the consequences of anthropogenic-mediated actions over marine biodiversity. This is particularly relevant nowadays, since the resilience of marine biodiversity to human perturbations can be exacerbated under a scenario of global climate change. Therefore, LMCEE aims at linking research efforts from such disciplines covering different levels of biodiversity organization into a solid scientific framework. The specific research lines of the group include:


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- To describe the biodiversity of marine invertebrates from deep-sea Portuguese territorial waters and from estuarine fauna.

- To describe patterns of spatial and temporal variation in the biodiversity costal marine habitat along the Atlantic coast of continental Portugal, with some particular case studies like the Minho Estuary, both through site-specific studies and by ecological modeling.

- To contribute to disentangle the complexity of trophic interactions in estuarine environments by traditional stomach content analysis, food preference investigations, through determination of stable isotopes (C and N) signatures across the food web, and by integrating the information into ecological modeling.

- To determine the effect of anthropogenic-mediated disturbances (such as sedimentation, nutrient enrichment, etc) over the organization and structure of marine and estuarine biodiversity, from individual populations to entire communities, therefore aiming to estimate the effects of changes in marine and estuarine biodiversity over the functioning of marine and estuarine ecosystems.

- To understand how marine and estuarine biodiversity respond to predicted changes in environmental variables (e.g. global warming and climate change) in combination with other concurrent stressors (e.g. eutrophication), using modeling tools.

- To apply molecular methodologies in biodiversity, population and conservation and evolutionary genetics to gain further understanding on historical and contemporary evolutionary forces that govern genetic variation in nature, i.e. the evolutionary relationships and life history variation of organisms.

- Improving the public awareness and understanding of links and specificity of taxonomy, ecology and evolution, and of the increasing need and urgency for applying a scientific approach integrating all these disciplines to biodiversity and conservation research.

- To contribute to the Integrated Coastal Zone Management (ICZM) by making Geographic Information Systems (GIS) tools available to stakeholders for data analysis, to identify spatial connections between different layers of information, and as a support of the estuarine and coastal management specially of the climate change effects in the estuarine and coastal area of the Minho Estuary.


As member of the FCT project "METALAND: Inferring Metapopulation Dynamics with Landscape Genetics for Vertebrates of Mauritanian Mountains" (PTDC/BIA-BEC 099934-2008), E Froufe processed many taxa for genetic analysis. This resulted in 1 publication (several more in prep.), including analysis of two comersal fishes, Tilapia sp, and Barbus sp.

As member of the FCT project "Diversification in the biomechanical and physiological performance of scorpions across biogeographic barriers" (PTDC/BIA-BEC 104644-2008), E Froufe processed several scorpions' taxa for genetic analysis, resulting in 1 publication (several more in prep.).


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A long-term study of the phylogeography of marine taxa in the Macaronesia, resulted in 2 publications.

Established combination of morphological and molecular techniques applied to the micro-crustaceans resulted in 1 publication.

Deep-sea collections from the Selvagem cruise (EMEPC) and from the Azores are screened for micro-crustaceans. Additional collections of micro-crustaceans from other Atlantic and Pacific deep-sea basins have been obtained from IFREMER, NHMUK, University of Bergen, Schenckenberg institution, and NIWA and screened for suitable target species.

A database of available deep-sea species was build for the BIODEEP FCT proposal.

A novel trap design are constructed for the collection, determination of population structure, recolonization potential and evaluation of potential as Bioindicator organisms of micro-crustaceans, for the BIOMES FCT proposal.

Shallow-water collection expeditions to multiple locations along the Portuguese coast (from Viana do Castelo to Sines) were conducted and the micro-crustaceans, sorted, identified, and processed for genetic analysis.

One new family and genus, four new deep-sea species as well as two new shallow-water species of micro-crustaceans, have been described and illustrated and the type-species of the most common shallow-water species have been redescribed from Mindelo.

Two suborder phylogenies of micro-crustaceans, have been published.

Site-specific and experimental data was collected for building population dynamics models and to contribute to Dynamic Energy Budgets model of key-stone species from Minho Estuary.

An hydrodynamic model for the Minho estuary was developed and is being validated and fine-tunned to simulate different scenarios of climate change and to analyse previewed effects in the hydrodynamic patterns of the area. The cross-analysis of these effects with population dynamics models allows for insight on possible climate change impacts at population level.

A Geographic Information Systems (GIS) database for the Minho estuary was built and is updated for use as an ICZM tool, on effects of climate change. It includes traditional geographic information as well as hydrodynamic and ecological information like the spatial and temporal species distribution.

The requirements to the vulnerability assessment to climate change risks of Minho River Estuary were investigated, including the legal and the institutional frameworks with co-interference in the management of the area.

As part of an EU network of research on marine biodiversity (LargeNet project of MarBEF), a database was build on plankton and benthos suitable to study large scale patterns and long-term changes.


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Collaborations with leading Portuguese and foreign scientists allowed for relevant studies which improved ecological knowledge of effects on climate events, introduced species, marine protected areas and biological interactions on intertidal and subtidal benthic assemblages. From these studies 10 publications are published (or in press) in high profile ecology and marine biology journals.

Participation of I Bertocci reinforced the involvement of CIIMAR to the EMEPC mission to Selvagens Islands, contributing to collections of biological data from rocky intertidal habitats.

8d. Productivity


1. Balata, D, Piazzi, L, Nesti, U, Bulleri, F, Bertocci, I. 2010. Effects of enhanced loads of nutrients on epiphytes on leaves and rhizomes of Posidonia oceanica. Journal of Sea Research 63: 173-179.

2. Bertocci, I, Maggi, E, Vaselli, S, Benedetti-Cecchi, L. 2010. Resistance of rocky shore assemblages of algae and invertebrates to changes in intensity and temporal variability of aerial exposure. Marine Ecology Progress Series 400: 75-86.

3. Bertocci, I, Arenas, F, Matias, M, Vaselli, S, Araújo, R, Abreu, H, Pereira, R, Vieira, R, Sousa-Pinto, I. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116.

4. Bulleri, F, Balata, D, Bertocci, I, Tamburello, L, Benedetti-Cecchi, L. 2010. The seaweed Caulerpa racemosa on Mediterranean rocky reefs: from passenger to driver of ecological change. Ecology 91: 2205-2212.

5. Campos, J, Bio, A, Cardoso, JFMF, Dapper, R, Witte, JIJ,Van der Veer, HW. 2010. Fluctuations on the brown shrimp Crangon crangon (Crustacea: Caridea) abundance in the western Dutch Wadden Sea, The Netherlands. Marine Ecology Progress Series 405: 203-219

6. Claudet, J, Osenberg, CW, Domenici, P, Badalamenti, F, Milazzo, M, Falcón, JM, Bertocci, I, Benedetti-Cecchi, L, García-Charton, J-A, Goñi, R, Borg, JA, Forcada, A, de Lucia, GA, Pérez-Ruzafa, Á, Afonso, P, Brito, A, Guala, I, Le Diréach, L, SanchezJerez, P, Somerfield, PJ, Planes, S. 2010. Marine reserves: fish life history and ecological traits matter. Ecological Applications 20: 830-839.

7. Dolbeth, M, Martinho, F, Freitas, V, Costa-Dias, S, Campos, J, Pardal, MA. 2010. Multi-year comparisons of fish recruitment, growth and production in two drought-affected Iberian estuaries. Marine and Freshwater Research 61: 1399-1415.

8. Incera, M, Bertocci, I, Benedetti-Cecchi, L. 2010. Effects of mean intensity and temporal variability of disturbance on the invasion of Caulerpa racemosa var. cylindracea (Caulerpales) in rock pools. Biological Invasions 12: 501-514.


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9. Freitas, V, Campos, J, Skreslet, S, Van der Veer, HW. 2010. Habitat quality of a subarctic nursery ground for 0-group plaice (Pleuronectes platessa L.). Journal of Sea Research 64: 26-33.

10. Freitas, V, Cardoso, JFMF, Lika, K, Peck, MA, Campos, J, Kooijman, SALM, Van der Veer, HW. 2010. Temperature tolerance and energetics, a Dynamic Energy Budget-based comparison of North Atlantic marine species. Philosophical Transactions of the Royal Society B 365: 3553-3565.

11. Schander, C, Rapp, HT, Kongsrud, JA, Bakken, T, Berge, J, Cochrane, S, Oug, E, Byrkjedal, I, Todt, C, Cedhagen, T, Fosshagen, A, Gebruk, A, Larsen, K, Levin, L, Obst, M, Pleijel, F, Stöhr, S, Warén, A, Mikkelsen, NT, Hadler-Jacobsen, S, Keuning, R, Petersen, KH, Thorseth, IH, Pedersen, RB. 2010. The fauna of hydrothermal vents on the Mohn Ridge (North Atlantic). Marine Biology Research 6(2): 155-171.

12. Sousa, P, Froufe, E, Alves, PC, Harris, DJ. 2010. Genetic diversity within Scorpions of the genus Buthus from the Iberian Peninsula: mitochondrial DNA sequence data indicate additional distinct cryptic lineages. Journal of Arachnology 38(2): 206-211.

13. Thomsen, MS, Wernberg, T, Altieri, A, Tuya, F, Gulbransen, D, McGlathery, K, Holmerk, M, Silliman, BR. 2010. Facilitation cascades via habitat formation and modification. Integrative and Comparative Biology 50:158-175.

14. Thomsen, MS, Wernberg, T, Tuya, F, Silliman, BR. 2010. Ecological performance and possible origin of a ubiquitous but under-studied gastropod. Estuarine Coastal and Shelf Science 87: 501-509.

15. Tuya, F, Larsen, K, Platt, V. 2010. Patterns of abundance and assemblage structure of epifauna inhabiting two morphologically different kelp holdfasts. Hydrobiologia 658(1): 373-382.

16. Tuya, F, Vanderklift, MA, Hyndes, GA, Wernberg, T, Thomsen, MS, Hanson, C. 2010. Proximity to reefs affects the balance between positive and negative effects on seagrass fauna. Marine Ecology Progress Series 405: 175-186.

17. Vandepitte, L, Vanhoorne, B, Kraberg, A, Anisimova, N, Antoniadou, C, Araújo, R, Bartsch, I, Beker, B, Benedetti-Cecchi, L, Bertocci, I, Cochrane, S, Cooper, K, Craeymeersch, J, Christou, E, Crisp, DJ, Dahle, S, de Boissier, M, de Kluijver, M, Denisenko, S, De Vito, D, Duineveld, G, Escaravage, V, Fleischer, D, Fraschetti, S, Giangrande, A, Heip, C, Hummel, H, Janas, U, Karez, R, Kedra, M, Kingston, P, Kuhlenkamp, R, Libes, M, Martens, P, Mees, J, Mieszkowska, N, Mudrak, S, Munda, I, Orfanidis, S, Orlando-Bonaca, M, Palerud, R, Rachor, E, Reichert, K, Rumohr, H, Schiedek, D, Schubert, P, Sistermans, WCH, Sousa Pinto, I, Southward, AJ, Terlizzi, A, Tsiaga, E, van Beusekom, J, Vanden Berghe, E, Warzocha, J, Wasmund, N, Weslawski, JM, Widdicombe, C, Wlodarska-Kowalczuk, M, Zettler, ML. 2010. Data integration for European marine biodiversity research: creating a database on benthos and plankton to study large-scale patterns and long-term changes. Hydrobiologia 644: 1-13.

18. Vasconcelos, R, Froufe, E, Brito, JC, Carranza, S, Harris, DJ. 2010. Phylogeography of Amietophrynus regularis based on mitochondrial DNA sequences: inferences regarding the Cape Verde population and biogeographical patterns. Journal of African Zoology 45(2): 291–298.


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19. Wernberg, T, Thomsen, MS, Tuya, F, Kendrick, GA, Staehr, PA, Toohey, BD. 2010. Decreasing resilience of kelp beds along a latitudinal temperature gradient: potential implications for a warmer future. Ecology Letters 13: 685-694.

20. Wernberg, T, Tuya, F, Thomsen, MS, Kendrick, GA. 2010. Turban snails as habitat for foliose algae: contrasting geographical patterns in species richness. Marine and Freshwater Research 61: 1237-1242.

In press

21. Froufe, E, Alexandrino, P, Cabezas, P, Pérez-Losada, M. (in press). Comparative phylogeography of three lobster species from the Macaronesia (NE Atlantic). Crustacean Issues.

22. Gonzalez-Wanguemert, M, Froufe, E, Alexandrino, P. (in press). Phylogeographical history of the white seabream Diplodus sargus (Sparidae):Implications for island biogeography. Marine Biology Research.

23. Vandeperre, F, Higgins, RM, Sánchez-Meca, J, Maynou, F, Goñi, R, Martín-Sosa, P, Pérez-Ruzafa, A, Afonso, P, Bertocci, I, Crec’hriou, R, D’Anna, G, Dimech, M, Dorta, C, Esparza, O, Falcón, JM, Forcada, A, Guala, I, Le Direach, L, Marcos, C, Ojeda-Martínez, C, Pipitone, C, Schembri, PJ, Stelzenmüller, V, Stobart, B, Serrão Santos, R. (in press). Effects of no-take area size and age of marine protected areas on fishery yields: a metaanalytical approach. Fish and Fisheries.


1. Larsen, K. 2010. Order Tanaidacea: Tanaids. In: Gordon, DP (Ed). Species 2000. The New Zealand Inventory of Biodiversity, Chapter 8, Subphylum, Crustacea, Volume 2, Canterbury, University Press, Christchurch, pp. 164-166.

2. Larsen, K, Froufe, E. 2010. Identification of polymorphic species within groups of morphologically conservative taxa: combining morphological and molecular techniques. In: Nimis, PL, Vignes Lebbe, R (Eds). Tools for Identifying Biodiversity: Progress and Problems – pp. 301-305. ISBN 978-88-8303-295-0.

3. Larsen, K (in press). Tanaidacea. In: McGraw-Hill Encyclopedia of Science & Technology.

4. Larsen, K. (in press). Tanaidacea. In: De Grave, S, Martin, J. (Eds). Crustacean Field Collecting and Preservation Techniques, Cambridge University Press.



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MASTER THESES

Araújo-Silva, Catarina de Lourdes. 2010. Taxonomia e aspectos ecologicos de Tanaidacea (crustacea: Peracarida) da plataforma continental e bancos oceanicos no Programa REVIZEE. Master thesis, University of Pernambuco, Recife, Brazil. Supervisor: Kim Larsen.





Collaboration with the Royal Netherlands Institute for Sea Research, Bodo University College, Vreij University of Amsterdam, University of Crete and University of Hamburg resulted in 3 publications. One PhD student is under the co-supervision with Bergen University. Collaboration is also undergoing with Tromso University.

One master student (supervisor Kim Larsen) completed her thesis and published two taxonomical publications. Collaboration between Dr. Larsen and the Zoological Museum of Copenhagen resulted in the publication of a new family and genus. Collaboration with NIWA, New Zealand resulted in publication of 1 suborder phylogeny. Collaboration with University of Grand Canaria resulted in many samples of macronesian micro-crustaceans.

Collaboration with the University of Pisa and CETMAR (Vigo) resulted in several publications on effects of climate-related disturbances and invasive organisms. Collaborations with the same team led to publications on the effects of eutrophication and mechanisms of succession of intertidal assemblages.

Collaboration with University of Salento and the team of the EU project EmpaFish resulted in a publication on the importance of fish life and ecological traits for the effectiveness of marine protected areas. Collaboration with Universidade dos Açores resulted in a publication on the effects of traits of marine protected areas on fishery yields.

Collaboration with L Vandepitte from VLIZ (Oostende, Belgium) and the LargeNet team (MarBEF network) resulted in the compilation and publication of a plankton and benthos dataset suitable to be used for assessing long-term and large-scale changes in marine biodiversity.

Collaborations with researchers from CIIMAR resulted in a publication on habitat-forming macroalgae and mechanical disturbance on intertidal habitats. Collaboration with CIMAR’s PhD student JP Oliveira resulted in a publication on effects of type and timing of disturbance on rock pool assemblages.


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196

Marine Plant Ecology (ALGAE)



Marine Plant Ecology (ALGAE)


Rui Orlando Pimenta Santos






- National funding

62.289 EUR, FCT, PTDC/AAC-CLI/103348/2008, METABOLISMO.

21.493 EUR, FCT, PTDC/MAR/098069/2008, INVASEA.

- European funding

25.784 EUR, European Commission, INTERREG SUDOE, SOE1/P2/F153, ECO-LAGUNES.


1. Objectives

I. Ecology of coastal systems:

The group aims to understand the population biology and ecophysiological processes that regulate the survival and production of marine vegetation, which are the basis of their ecological goods and services. We aim to reveal the importance of biological communities dominated by macrophytes for the carbon and nitrogen metabolism of coastal systems, in particular the environmental drivers of photosynthesis and community production, respiration and calcification. The nitrogen metabolism of seagrasses and how this may determine the nitrogen cycle of coastal systems is also a group objective.


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II. Human impacts on coastal ecosystems:

This research line focuses on the effects of human-related disturbances on costal ecosystems, including physical disturbances, increased nitrogen loads, alterations of the water quality parameters that determine seagrass productivity and distribution, and global change effects such as temperature and CO2/acidification.

Within the Water Framework Directive (WFD), we aim to use macroalgae and marine angiosperms as biological elements to classify the ecological status of the Portuguese coastal and transitional water bodies. A specific objective is to investigate biological indicators of seagrasses and seaweeds that can be used to assess the condition of those communities. As well, we aim to understand the general decline of seagrasses in the coastal systems, particularly the human-induced shifts from seagrasses to seaweed domination.

III. Economic valorisation of seaweeds

Following the applied research of last years that demonstrated an integrated cultivation system in a fish farm for the production of biologically active halogenated compounds from Asparagopsis spp. with commercial application in the cosmetics industry, we aimed to investigate how to improve the concentration of such compounds in the produced biomass. We also aimed at revealing novel potential applications of halogenated metabolites: the use of waterborne seaweed metabolites to provide an alternative technology to antibiotics in different stages of intensive fish production, linking for the first time, quantitative seaweed chemical analysis with molecular microbial techniques.


In 2010 we published 6 papers and presented 14 communications at international scientific meetings.

Some of our scientific contributions focused on the reproductive biology of Zostera marina, an endangered species at Ria Formosa. Its low germination and seedling survival rates suggest a bottleneck in the species’ reproductive cycle that may account for its decline in this system. On the other hand, the uptake rates of inorganic nitrogen of the most important seagrass species of Ria Formosa, Zostera noltii, showed that ammonium is its preferential inorganic nitrogen source. However, in the absence of ammonium the species may also uptake nitrate at high rates. The activity of key enzymes for nitrogen uptake was much higher in the leaves than in the roots, highlighting the importance of the leaves as primary reducing sites in the nitrogen assimilation process. Our research on the population dynamics of the third seagrass species present at Ria Formosa, Cymodocea nodosa, revealed that the net recruitment of all meadows, which were under different sedimentary regimes, was negative. If environmental conditions will remain the same, all these meadows are predicted to decline to extinction.

An important theoretical contribution was made to understanding the demographic mechanisms that determine the dynamics of the relative abundance of phases in biphasic life cycles, unifying previous numerical and observational attempts at this question. We showed how the three co-occurring population processes—growth, fertility, and looping—may dominate the dynamics of the population, determining both its growth rate and ploidy ratio.


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In the ambit of the economical valorisation of seaweeds we showed that the red alga Asparagopsis taxiformis cultivated in Southern Portugal in an integrated aquaculture system using fish farm effluents shows the highest production rates ever reported for cultivated macroalgae. The biological active halogenated metabolites that Asparagopsis spp. produce may be enhanced by the addition of H2O2 to the cultures, prior (3 h) to biomass harvesting.

In 2010, the following national-funded projects started under the coordination of our group:

Whole-system metabolism and CO2 fluxes in a coastal lagoon dominated by saltmarsh and seagrass meadows, PTDC/AAC-CLI/103348/2008

Shifts from seagrass to seaweed dominated systems (INVASEA), PTDC/MAR/098069/2008

We also participate in the national-funded project “Landscape genetics of a coastal lagoon; an empirical and modeling approach using the seagrass Zostera noltii in Ria Formosa, PTDC/MAR/099887/2008

The COST Action network ES0906 “Seagrass productivity: from genes to ecosystem management”, chaired by the coordinator of the research group officially started in March 15 2010. In July 1 2010 the CCMAR started its role as Grant Holder, to financially manage the Action.

Under the Association of European Marine Biological Laboratories financed by the European Community (ASSEMBLE – EC/FP7/227799) we improved and optimized the seagrass mesocosm cultivation system that started to be constructed in 2009 in the Ramalhete Field Station of CCMAR. During 2010 we were able to maintain the 3 species of seagrasses present at Ria Formosa lagoon, Zostera noltii, Z. marina and Cymodocea nodosa, for many months in good conditions. An experimental assessment of the effects of enriched CO2 on seagrass photosynthesis, production and nitrogen uptake was made during 2010. The system is now prepared to assess experimentally the effects of both CO2 and nitrogen enrichment on seagrasses.

8d. Productivity


1. Cabaço, S, Ferreira, Ó, Santos, R. 2010. Population dynamics of the seagrass Cymodocea nodosa in Ria Formosa lagoon following inlet artificial relocation. Estuarine, Coastal and Shelf Science 87: 510-516.

2. Cabaço, S, Santos. R. 2010. Reproduction of the eelgrass Zostera marina at the species southern distributional limit in the Eastern Atlantic. Marine Ecology 31: 300-308.

3. Alexandre, A, Silva, J, Santos, R. 2010. Inorganic nitrogen uptake and related enzymatic activity in the seagrass Zostera noltii. Marine Ecology 31(4): 539-545.


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4. Mata, L, Schuenhoff, A, Santos R. 2010. A direct comparison of two seaweed biofilters: Asparagopsis armata and Ulva rigida. Journal Applied Phycology 22: 639-644.

5. Vieira, V, Santos,R. 2010. Demographic mechanisms determining the dynamics of the relative abundance of phases in biphasic life cycles. Journal of Phycology 46: 1128-1137.

In press

6. Mata, L, Gaspar, H., Santos, R. (in press). Effects of hydrogen peroxide on the content of major volatile halogenated compounds in the red alga Asparagopsis taxiformis (Bonnemaisoniaceae). Journal Applied Phycology, doi: 10.1007/s10811-010-9582-y.




MASTER THESES

Guerra, Luís Miguel. 2010. Análise multivariada da evolução sucessão dunar na Península do Ancão e Ilha da Barreta. Master thesis, University of Algarve. Supervisors: Rui Santos, Vasco Vieira.

Mateus, Carmen Sofia Cardoso. 2010. Análise do efeito da halófita Salicornia ramosissima na remoção de nutrientes em substrato sólido e em meio hidropónico. Master thesis, University of Algarve. Supervisors: Luís Chícharo, Rui Santos.





Even though only 1 in 7 published papers in 2010 were in collaboration with foreign colleagues (14%), 10 of the 15 communications (67%) made in international scientific meetings were done in collaboration with international research teams. Eventually, some of these will be published in the near future.


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The group leader is the chair of COST Action ES0906, “Seagrass productivity: from genes to ecosystem management”. This network aims to improve the scientific collaboration among European research groups and to bridge the gap between scientists and seagrass managers. It involves 19 European countries and over a hundred researchers from Europe, Australia and USA.

All the group’s new approved projects involve foreign partners and/or institutions. The same applies for all the pending proposals. Active collaborations are currently underway with European partners from Stockholm University, Stazione Zoologica Anton Dohrn, Università Tor Vergata, Universidad de Cádiz and Universidad de A Coruña. These collaborations take form as partnerships in projects, graduate and undergraduate student reciprocal training, and collaborative informal research. Overseas partners include NOAA (USA), the Smithsonian Environmental Research Center (USA) and Tel Aviv University (Israel). All overseas partners are involved in ongoing and pending projects.

We play an active role on the Association of European Marine Biological Laboratories financed by the European Community (ASSEMBLE – EC/FP7/227799), particularly on the development and maintenance of seagrass mesocosms at CCMAR, and interacting with visiting scientists that apply to use this facility.

Team members integrate Editorial Boards of indexed international scientific journals such as Marine Ecology and Marine Environmental Research. The group leader is an Associate Editor of Estuaries and Coasts. The team members regularly act as referees for many international scientific journals.

The group leader was the chair of a mini-symposium session at the XX International Seaweed Symposium, Ensenada, Mexico. He was also the chair of the students presentations evaluation board in the World Seagrass Conference, Phuket, Thailand.

The research group is responsible for the seasonal monitoring of two sites in Ria Formosa lagoon, for the world-wide SeagrassNet monitoring program (www.seagrassnet.org).


A report was prepared for the Water Institute of Portugal (INAG) on the assessment of the biological quality elements, macroalgae, seagrasses and saltmarshes of the coastal and transition waters of the Portuguese coast, in the ambit of the project “EEMA: Ecological status of coastal and transitional waters of the Portuguese coast, under the Water Framework Directive”.

http://www.seagrassnet.org/


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Microbial Ecology and Evolution



Microbial Ecology and Evolution


Rodrigo da Silva Costa






- National funding

93.13 EUR, FCT, PTDC/AAC-CLI/107916/2008, Global climate change and pollution: a synergy designed for disaster?.

80.882 EUR, FCT, PTDC/MAR/101431/2008, Exploring the bacterial diversity encrypted in the metagenomes of marine sponges.


1. Objectives

The Microbial Ecology and Evolution Research Group seeks to understand the causes and consequences of microbial diversity in nature. We apply molecular tools to unravel the composition and function of microbial consortia in natural settings and microcosms. This enables us to investigate the driving forces that shape microbial community structure in the open environment and under experimental control. Current research addresses the molecular ecology of bacteria in marine sponges and the response of the microbiota of marine sediments to global change and pollution.


A main goal and concrete achievement was the structuring of a new laboratory to completion. This new lab is equipped with state-of-the-art instruments and is suited for microbial


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metagenomics / molecular ecology research. Furthermore, in 2010 the group has kept a fair scientific productivity while investing in infrastructure, established novel national and international collaborations, launched two national research projects, acquired more research funds for 2011 and significantly invested in scientific diffusion and training of under graduated students. The aforementioned activities are more precisely highlighted below.

Infrastructure

- Creation of a functional metagenomics /molecular biology laboratory in the first semester of 2010;

Research

- Startup of two national projects;

- Three publications in peer reviewed international journals;

- Research fund acquisition for 2011 through collaboration in two novel projects.

International collaborations

- Host of two foreign working groups under the ASSEMBLE programme: Dr. Jan Dirk van Elsas (University of Groningen, The Netherlands) and Dr. Thomas Wichard (Friedrich-Schiller Jena University, Germany);

- All 2010 publications resulted from international cooperation with research groups from Germany, The Netherlands, Brazil, and Portugal, among others.

Education and training

- Co-supervision of three Master students: Catarina Cúcio (Supervision: Marine Ecology and Evolution, CCMAR); 2. Ana Cristina Castanheira (Supervision: Marine Ecology and Evolution, CCMAR); 3. Diana Sofia Conduto António (Supervision: Prof. Dr. Margarida P. Reis, FCT, Univ Algarve).

- Supervision of one Ph.D. student: Cristiane CP Hardoim, Research grant: SFRH/ BD/ 60873/ 2009.

Scientific diffusion actions

- The group has been represented in many venues, both in Europe and overseas (see below). In 2010, focus was given to introducing the group itself and its current research to a broad and international audience. It is felt that a more intensive dissemination campaign is needed within CCMAR and Univ Algarve. The group regularly releases its research outcomes on Facebook. As from 2011 we will cross-feed the more relevant information posted on this page with the CCMAR webpage. We plan to contribute with at least two oral presentations at the internal seminar organized by CCMAR.


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8d. Productivity


1. Dini-Andreote, F, Andreote, FD, Costa, R, Taketani, RG, van Elsas, JD, et al. 2010. Bacterial soil community in a Brazilian sugarcane field. Plant and Soil 336: 337-349.

2. Gomes, NCM, Flocco, CG, Costa, R, Junca, H, Vilchez, R, et al. 2010. Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia. Fems Microbiology Ecology 74: 276-290.

3. Van Elsas, JD, Semenov, A, Costa, R, Trevors, J. 2011. Survival of Escherichia coli in the environment: fundamental and public health aspects. The ISME Journal 5: 367 (published on line in June 2010, doi: 10.1038/ismej.2010.80).








Host of two foreign working groups under the ASSEMBLE programme: Dr. Jan Dirk van Elsas (University of Groningen, The Netherlands) and Dr. Thomas Wichard (Friedrich-Schiller Jena University, Germany);

All 2010 publications resulted from international cooperation with research groups from Germany, The Netherlands, Brazil, and Portugal, among others.



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Modelling and Coastal Management



Modelling and Coastal Management


Fernando Francisco Machado Veloso Gomes





- European funding

ANCORIM - Atlantic Network for Coastal Risk Management, Interreg Atlantic Area, 2009-2011. IHRH.

DEWFORA - Improved Drought Early Warning and FORecasting to preparedness and adaptation to droughts in Africa

- National funding

FCT PTDC/ECM/100887/2008




- Two service contracts with the National Water Authority (INAG), Ministry of Environment.


1. Objectives

Wave prediction and interaction:

https://www.fe.up.pt/si/projectos_geral.mostra_projecto?P_ID=1923



http://www.fe.up.pt/si/projectos_geral.mostra_projecto?P_ID=1932


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- Computational and physical modelling techniques to predict near shore wave field to provide site-specific wave climate and extreme conditions and the interaction of waves, currents and solid boundaries.

Coastal impact assessment:

- Medium and long modelling and field studies to investigate the impact of harbours structures, coastal protection structures, dredging activities, artificial sand nourishment on beaches and dunes, considering scenarios of climate change and variability. Use of remote sensing data. New technical solutions for coastal protection.

Integrated coastal zone management:

- Research, consultancy and support in coastal risk assessment and management, coastal, port and estuary Master Plans, Integrated Coastal Management Strategy, Climate Change.

Water resources management:

- Extreme events, with special focus on water stress, scarcity and droughts and development of indicators. Application of decision support system tools for testing and evaluation of different water stress mitigation options. EU and WFD policy implementation, with emphasis on shared river issues and special focus on bilateral Iberian shared river basins. Stakeholder’s involvement and public participation. Influence of the operation and management of urban water systems in water resources availability.

Sediment transport and river hydraulics:

- Sediment transport studies, employing new analytical and experimental technologies. Urban rivers rehabilitation methods, embracing engineering and environmental techniques. Computational modelling of river hydraulic and aquifers behaviour, assessing quantity and quality issues.


Wave prediction and interaction:

- Computational and physical modelling (Wave tank of Faculty of Engineering).

Coastal impact assessment:

- Improvement of LTC model (medium and long term modelling considering scenarios of climate change and variability). Experimental evaluation of new coastal protection solutions. Conclusion of two PhD theses. Two new pos-doc research.


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Integrated coastal zone management:

- More than ten scientific and technical reports delivered to the Ministry of Environment (National Water Authority INAG).

Water resources management:

- Conclusion of an EU project. New EU project. Scientific and technical reports delivered to the Ministry of Environment (National Water Authority INAG). Conclusion of one PhD thesis.

Sediment transport and river hydraulics:

- Urban rivers rehabilitation methods, embracing engineering and environmental techniques. Conclusion of one PhD thesis.

8d. Productivity


In press

1. Taveira Pinto, F, Rosa Santos, P, Veloso Gomes, F, Guedes Lopes, H. (in press). Efficiency analysis to reflection of a new quay wall type, Journal of Hydraulic Research, Maritime Special Issue, TJHR 574378.

2. Teodoro, AC, Pais-Barbosa, J, Gonçalves, H, Veloso-Gomes, F, Taveira-Pinto, F. (in press). Identification of beach features/patterns through image classification techniques applied to remotely sensed data, International Journal of Remote Sensing.

3. Correia, L, El-Ahmadi, A, Molle, B, Maia, R, Oliveira, I. (in press). Benchmark Irrigation Practices on Olives using a Structural Equation Modeling Approach. Irrigation and Drainage.


1. Pais-Barbosa, J, Veloso-Gomes, F, Taveira Pinto, F. 2010. GIS Applications for Coastal Morphodynamics' Analysis of the Portuguese West Coast, Coastal and Marine Geospatial Technologies (book series: Coastal Systems and Continental Margins), Ed Green, DR, Springer, pp. 275-283.


1. Pais-Barbosa, J, Veloso-Gomes, F, Taveira Pinto, F. 2010. Análise de formas e padrões costeiros como indicadores de erosão na zona costeira noroeste de Portugal. Revista da Associação Portuguesa de Recursos Hídricos, APRH, Volume 31#2, pp. 15-26.


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2. Petan, S, Taveira-Pinto, F, Mikoš, M, Pais-Barbosa, J. 2010. Modelação da erosão do solo da bacia hidrográfica do Rio Leça, com a Equação RUSLE e SIG. Revista da Associação Portuguesa de Recursos Hídricos, (APRH), Volume 31#1, pp. 99-110.

3. Vivas, E, Maia, R. 2010. A Gestão de Escassez e Secas enquadrando as Alterações Climáticas. Revista Recursos Hídricos, Volume 31, nº 1, Março de 2010.


PhD THESES

1. Santos, Paulo Jorge Rosa. 2010. Análise da Interacção de Navios com Dispositivos de Acostagem e Amarração. Estudo em Modelo Físico do Posto “A” do Terminal de Petroleiros do Porto de Leixões. PhD thesis in Civil Engineering, Faculty of Engineering, University of Porto. Supervisor: Fernando Veloso Gomes, Co-supervisor: Francisco Taveira Pinto.

2. da Silva, Raquel Castro Alves Ferreira. 2010. Avaliação Experimental e Numérica de Parâmetros Associados a Modelos de Evolução da Linha de Costa. PhD thesis in Civil Engineering, Faculty of Engineering, University of Porto. Supervisor: Fernando Veloso Gomes, Co-supervisor: Francisco Taveira Pinto.






- Contract with the Ministry of Environment (INAG / FEUP): coastal consultancy and monitoring.

- Contract with the Ministry of Environment (INAG / FEUP): water stress, scarcity and droughts management.


208

Nutrition, Growth and Quality of Fish (LANUCE)



Nutrition, Growth and Quality of Fish (LANUCE)


Luísa Maria Pinheiro Valente




- 21.000 EUR, FCT Pluriannual funding

- National funding

135.000 EUR (CIIMAR), FCT PTDC/MAR/105229/2008, BenefitS - Benefícios da produção de macroalgas marinhas em sistemas de aquacultura integrada multitrófica. Total funding: 170.000 EUR. PI: IS Pinto (CIIMAR).

128.000 EUR (CIIMAR), FCT PTDC/MAR/098035/2008, Fisiologia da regulação de iões e ferro na lampreia marinha (Petromyzon marinus) - um parasita. Total funding: 154.000 EUR, 2009-2012. : PI: J Wilson (CIIMAR).

45.000 EUR (CIIMAR), AdI, QREN, PROAMBIENTE- Sustainable and environmental friendly aquafeeds for Senegalese sole. In consortium with the company A. Coelho e Castro, Lda. Total funding: 591.000 EUR, 2009-2012. PI: L Valente.

188.000 EUR (CIIMAR), FCT PTDC/MAR/70858/2006, TEXBREAM - Post-mortem changes in gilthead sea bream muscle proteins: its implications to flesh texture, 2008-2011. Total funding: PI: J Dias (CCMAR).

40.000 EUR (CIIMAR), QREN, ECOPISCIS - Waste Management in Fish Production, project 3442. Total funding: 591.369 EUR, 2009-2012. PI: JF Gonçalves (ICBAS), R Ozório.

40.000 EUR (CIIMAR), AdI, QREN, PROBIOSOLEA - Selecção e avaliação de estirpes de probioticos para uso na aquacultura do linguado (Solea senegalensis), project 13551. Total funding: 550.000 EUR. PI: R Ozório.


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1. Objectives

The central aim of the group is to contribute to the sustainable development of the aquaculture The central aim of the group is to contribute to the sustainable development of the aquaculture industry, through basic and applied research directed to the optimisation of diets and cultivation techniques in order to improve growth and muscle quality. Special attention has been given to the introduction of new species to aquaculture, namely the blackspot seabream (Pagellus bogaraveo), the sole (Solea senegalensis), the bream (Diplodus sargus) and the octopus (Octopus vulgaris).


During the course of 2010, LANUCE group has published 28 papers in international peer review journals, 4 scientific reports, has presented 27 oral and 29 poster communications. In addition, LANUCE has concluded 3 PhD thesis, 2 MSc thesis and collaborated in the organization of 3 International meetings. The achievements mentioned above is a result of the scientific outcome that described below.

Nutrient requirements, dietary formulations and feeding practices were optimized for Senegalese sole and bream. Optimal growth of sole is obtained with 8% lipids; the estimated lysine requirement (4.68 g lysine / 16 g N) was used to design diets using plant protein sources. The great acceptability of plant in Senegalense sole was demonstrated.. Several projects are still going on to test plant protein diets during ongrowing stages and clarify the impact of lipids on sole metabolism (7 papers, 1 PhD student).

The production of ornamental aquatic species has now evolved into an industrial activity. Information regarding nutritional requirements, food utilization and behaviour of ornamental fish were considered.

Understanding the environmental factors that regulates muscle differentiation and growth is important to select the best strategy to optimize growth and meat quality The muscle growth potential and its relation to cellularity and sensorial characteristics were evaluated in several species and rearing conditions. SEACASE EU projected leaded by CCMAR clearly demonstrated that it is possible to discriminate products from distinct rearing systems. (3 papers)

LANUCE just started a project on the evaluation of epigenetic factors on muscle growth and differentiation in Senegalse sole (1 PhD student).

In intensive marine fish production, the large quantity of disinfectants and drugs released into the rearing water can negatively affect the integrity of the gastrointestinal microflora, thereby reducing growth and hindering the immunologic status of the fish. Until now, antibiotics have been the most employed agents in the treatment of diseases in aquaculture; however, the environmental and food contamination resulting from its use, as well as the development of pathogens with increased antimicrobial resistance constitute emerging problems which need effective and urgent solutions. Probiotics may inhibit the colonization of pathogen bacteria by various mechanisms. The reduction of stress-related impact by probiotics is expected to improve growth performance and reduce acute as well as chronic stress. Output: PhD: 2 (in course).


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Crustaceans that are traded alive were studied, with the objectives of characterization of the main species, reducing the high mortality rates during handling and transportation and improvement of general welfare (1 PhD thesis finished, 9 articles + 1 submitted).

Different approaches were studied to obtain improvements and added value for fish product industrial waste, namely in preservation and gelification properties of fish minces (1 PhD thesis in finishing phase, 7 articles published + 1 submitted).

Products obtained from salted/dried cod, namely rehydrated frozen products recently introduced in the seafood market of countries like Portugal, are being studied. Main objective is to improve the industrial procedures, reducing processing times and costs (1 PhD thesis started in 2010).

Sensory methods for seafood evaluation continue to be one of the working areas, in 2010, by giving external support to a PhD thesis under development at Buenos Aires, Argentina, for common carp (Cyprinus carpio) (1 PhD thesis, advanced phase).

8d. Productivity


1. Antunes, F, Hinzmann, M, Lopes-Lima, M, Machado, J, Martins da Costa, P. 2010. Relationship between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of Anodonta cygnea (Linnaeus, 1758). Microbial Ecology 60 (2): 304-309.

2. Araújo, C, Torres, C, Silva, N, Carneiro, C, Gonçalves, A, Radhouani, H, Correia, S, da Costa, PM, Paccheco, R, Zarazaga, M, Ruiz-Larrea, F, Poeta, P, Igrejas, G. 2010. Vancomycin-resistant enterococci from Portuguese wastewater treatment plants. Journal of Basic Microbiology 50: 605-609.

3. Barrento, S, Marques, A, Teixeira, B, Mendes, R, Bandarra, N, Vaz-Pires, P, Nunes, ML. 2010. Chemical composition, cholesterol, fatty acid and amino acid in two populations of brown crab Cancer pagurus: Ecological and human health implications. Journal of Food Composition and Analysis 23(7): 716-725.

4. Barrento, S, Marques, A, Vaz-Pires, P, Nunes, ML. 2010. Live shipment of immersed crabs Cancer pagurus from England to Portugal and recovery in stocking tanks: stress parameter characterization. ICES Journal of Marine Science 67: 435-443.

5. Bertocci, I, Arenas, F, Matias, M, Vaselli, S, Araújo, R, Abreu, H, Pereira, R, Vieira, R, Sousa-Pinto, I. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116.

6. Campos, C, Valente, LMP, Borges, P, Bizuayehu, ST, Fernandes, JMO. 2010. Dietary lipid levels have a remarkable impact on the expression of growth-related genes in Senegalese sole (Solea senegalensis Kaup). Journal of Experimental Biology 213: 200-209.


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7. Cardoso, C, Mendes, R, Pedro, S, Vaz-Pires, P, Nunes, M. 2010. Quality changes during storage of minced fish products containing dietary fibre and fortified with ω3 fatty acids. Food Science and Technology International 16(1): 131-142.

8. Cardoso, C, Mendes, R, Vaz-Pires, P, Nunes, ML. 2010. Effect of salt and MTGase on the production of high quality gels from farmed sea bass. Journal of Food Engineering 101: 98-105.

9. Cardoso, CL, Mendes, RO, Salvador, AM, Saraiva, JA, Vaz-Pires, P, Nunes, ML. 2010. Quality characteristics of high pressure-induced hake (Merluccius capensis) protein gels with and without MTGase. Journal of Aquatic Food Product Technology 19: 193-213.

10. Dias, J, Yúfera, M, Valente, LMP, Rema, P. 2010. Feed transit and apparent protein, phosphorus and energy digestibility of practical feed ingredients by Senegalese sole (Solea senegalensis). Aquaculture 302: 94-99.

11. Figueiredo-Silva, AC, Corraze, G, Kaushik, S, Peleteiro, JB, Valente, LMP. 2010. Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids. Amino acids 39: 1401-1416.

12. Figueiredo-Silva, AC, Corraze, G, Valente, LMP. 2010. The growth and nutrient utilisation of blackspot seabream (Pagellus bogaraveo) under different feeding regimes. Fish Physiology and Biochemistry 36: 1113-1124.

13. Figueiredo-Silva, AC, Corraze, G, Borges, P, Valente, LMP. 2010. Dietary protein/lipid level and protein source effects on growth, tissue composition and lipid metabolism of Blackspot seabream (Pagellus bogaraveo) Aquaculture Nutrition 16: 173-187.

14. Gonçalves, JFM, Turini, BGS, Ozório, ROA. 2010. The effects of stocking densities on performance of turbot (Scophthalmus maximus) fed diets with L-carnitine supplementation. Scientia Agricola 67(2): 151-157.

15. Martins da Costa, P, Bica, A, Vaz-Pires, P, Bernardo, F. 2010. Changes in antimicrobial resistance among faecal enterococci isolated from growing broilers prophylactically medicated with three commercial antimicrobials. Preventive Veterinary Medicine 93: 71-76.

16. Nogueira, N, Cordeiro, N, Canada, P, Cruz e Silva, P, Ozório, ROA. 2010. Separate and combined effects of cyclic fasting and L-carnitine supplementation in red porgy (Pagrus pagrus, L. 1758). Aquaculture Research 41: e795–e806.

17. Ozorio, ROA, Van Ginneken, VJT, Bessa, RJB, Verstegen, MWA, Verreth, JAJ, Huisman, EA. 2010. Effects of exercise on L-carnitine and lipid metabolism in African catfish (Clarias gariepinus) fed different dietary L-carnitine and lipid levels. British Journal of Nutrition 103: 1139-1150.

18. Ozório, ROA, Turini, BGS, Moro, G, Oliveira, LST, Portz, L, Cyrino, JEP. 2010. Growth, nitrogen gain and indispensable amino acid retention of pacu (Piaractus mesopotamicus, Holmberg 1887) fed different brewers yeast (Saccharomyces cerevisiae) levels. Aquaculture Nutrition 16: 276-283.


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19. Pinto, L, Radhouani, L, Coelho, C, Martins da Costa, P, Simões, R, Brandão, RML, Torres, C, Igrejas, G, Poeta, P. 2010. Genetic detection of extended-spectrum {beta}-lactamases Escherichia coli in birds of prey from Serra da Estrela Natural Reserve of Portugal. Applied and Environmental Microbiology 76(12): 4118-20.

20. Seixas, P, Otero, A, Valente, LMP, Dias, J, Rey-Méndez, M. 2010. Growth and fatty acid composition of Octopus vulgaris paralarvae fed with enriched Artemia or co-fed with an inert diet. Aquaculture International 18: 1121-1135.

21. Seixas, P, Rey-Méndez, M, Valente, LMP, Otero, A. 2010. High DHA content in Artemia is ineffective to improve Octopus vulgaris paralarvae rearing. Aquaculture 300: 156-162.

22. Silva, JMG, Espe, M, Conceição, LEC, Dias, J, Costas, B, Valente, LMP. 2010. Feed intake and growth of Senegalese sole (Solea senegalensis, Kaup 1858) fed diets with partial replacement of fish meal with plant proteins. Aquaculture Research 41: e20-e30.

23. Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the myosin light chains 1,2 and 3 in the muscle of blackspot seabream, Pagellus bogaraveo, during development. Fish Physiology and Biochemistry 36: 1125-1132.

24. Simões, R, Poirel, L, Martins da Costa, P, Nordmann, P. 2010. Seagulls and beaches as a reservoir for emerging extended spectrum beta-lactamase producers in Escherichia coli. Emerging Infectious Diseases 16(1): 110-112.

25. Valente, LMP, Olmedo, M, Álvarez-Blázquez, B, Borges, P, Soares, S, Gomes, EFS, Pazos, G, Linares, F. 2010. Dietary protein and carbohydrate source on growth, body composition and tissue lipid deposition of blackspot seabream, Pagellus bogaraveo (Brunnich). Animal Physiology and Animal Nutrition 94: 212-219.

In press

26. Abreu, MH, Pereira, R, Yarish, C, Buschmann, AH, Sousa-Pinto, I. (in press). Aquaculture, doi: 10.1016/j.aquaculture.2010.12.036

27. Martins da Costa, P, Oliveira, M, Ramos, B, Bernardo, F. (in press). Livestock Science, doi:10.1016/j.livsci.2010.09.016.

28. Oliveira, M, Vieira-Pinto, M, Martins da Costa, P, Vilela, CL, Martins, C, Bernardo, F. (in press). Food Research International, doi: 10.1016/j.foodres.2010.08.011.

29. Silva, P, Valente, LMP, Olmedo, M, Álvarez-Blázquez, B, Galante, MH, Monteiro, RAF, Rocha, E. (in press). Aquaculture Research, doi: 10.1111/j.1365-2109.2010.02627.x.


1. Pereira, R, Yarish, C. 2010. The role of Porphyra in sustainable culture systems: Physiology and Applications. In: Israel, A, Einav, R, Seckbach, J (Eds). Role of Seaweeds in Globally Changing


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Environments, Cellular Origin, Life in Extreme Habitats and Astrobiology, 15. Springer Publishers, pp. 339-353.

2. Moraes, J, Iwashita, M, Ozório, R, Rema,, P, Moraes, F. 2010. Influence of Vitamin E Supplementation on Dermal Wound Healing in Tilapia, Oreochromis Niloticus. In: De Carlo, F, Bassano, A. Freswater Ecosystems and Aquaculture Research. Nova Science publishers, Inc. New York, pp. 177-192. ISBN 978-1-60741-707-1.

3. Dias, J, Conceição, LEC, Rodrigues, V, Colen, R, Ramalho Ribeiro, A, Ferreira, H, Aires, T, Silva, JMG, Escórcio, C, Valente, LMP. 2010. Improving the environmental sustainability of aquafeeds for gilthead seabream and Senegalese sole. Aquaculture Europe 35 (2): 5-11.

4. Quental-Ferreira, H, Ribeiro, AR, Dias, J, Yúfera, M, Arias, AM, Falcão, M, Serpa, D, Aires, T, Pousão-Ferreira, P, Cunha, ME, Valente, LMP. Dinis, MT, Conceição, L. 2010. Sustainable Semi-intensive polyculture of seabream and sole in earth ponds. Aquaculture Europe 35 (3): 17-21.

5. Vaz-Pires, P, Ramalho, A, Jesus, A, Soares, F, Conceição, L, Dinis, MT, Ribeiro, L, Pousão-Ferreira, P, Cunha, ME, Quental Ferreira, H, Yúfera, M, Marino, G, Boglione, C, Cataudella, S, Hussenot, J, Begout, ML, Makridis, P, Buard, E, Blachier, P. 2010. A Joint european certification system for sustainable non-intensive aquaculture: a proposal from the SEACASE project. Aquaculture Europe 35(2): 17-22.


7. Ozorio, ROA, Hubbard, PC, Barata, EN, Valente, LMP, Canário, AVM. 2010. Olfactory sensitivity to amino acids in the blackspot seabream (Pagellus Bogaraveo, Brünnich 1768): How effective is the electro-olfactogram in seawater?. Aquaculture Europe 2010, 5-10 October, Porto, Portugal. European Aquaculture Society, pp. 946-947.



MASTER THESES

Mesquita, João Rodrigo GG. 2010. Norovirus Contamination of shellfish from high sanitary production areas of Portugal. Master thesis in Sciences of Engineering / Zootechnical Engineering, University of Tas-os-Montes and Alto Douro.

Lino, Filipe Correia. 2010. Oxygen consumption experiments in the Pepper, furrow shell - Scrobicularia plana (da Costa) under starvation conditions - DEB model parameter estimation. Master thesis in Sciences of Engineering / Zootechnical Engineering, University of Tas-os-Montes and Alto Douro.


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PhD THESES

Barrento, Sara. 2010. Nutritional quality and physiological responses to transport and storage of live crustaceans traded in Portugal. PhD thesis in Animal Science, ICBAS, University of Porto. Supervisor: Maria Leonor Nunes (IPIMAR), Co-supervisor: Paulo Vaz-Pires.

Silva, Amélia Cláudia Figueiredo. 2010. Nutritional regulation of lipid deposition in blackspot seabream (Pagellus bogaraveo). PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa M.P. Valente, Co-supervisoror: Geneviéve Corraze (INRA, France).

Silva, Joana Margarida Guimarães. 2010. Use of alternative protein sources in diets for senegalese sole (Solea senegalensis Kaup, 1858) juveniles. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa M.P. Valente, Co-supervisoros: Marite Espe (NIFES, Norway), Luís Conceição.



European Aquaculture Society Meeting “Aquaculture Europe 2010 - Seafarming Tomorrow”, 5-8 October 2010, Porto, Portugal. L Valente - Member of the Local Organizing Committee.

COST FA0801 meeting “Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network (LARVANET)”, 5-6 October, porto, Portugal. L Valente - Member of the Local Organizing Committee (50 participants of 16 countries).

Symposium “Muscle development and growth”, integrated in the “9th International Congress on the Biology of Fish”, American Fisheries Society, 5-8 July 2010, Barcelona, Spain. L Valente - Member of the Scientific Committe.


Collaboration protocol with a feed and aquaculture company A. Coelho e Castro, for the development of dietary formulations for Senegalese sole, reduction of the environmental impact and assurance of fish quality (Project QREN, Support to investigation of applied research in companies, 2009-2012).

LANUCE together with ICBAS/UP coordinate the ECOPISCIS project in consortium with a Portuguese aquaculture enterprise Quinta-do-Salmão Ltd. (Project QREN 3442). The aim of ECOPISCIS is to develop an environmental friendly, long-run alternative to minimize biological waste from fish production.

LANUCE together with ICBAS/UP coordinate the PROAMBIENTE project in consortium with a Portuguese aquaculture enterprise Quinta-do-Salmão Ltd. (Project QREN 5362). The aim of


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PROAMBIENTE is to develop production technologies of sole in a sustainable manner, reducing the environmental impact, improving the well-being and the product quality of the animals.

LANUCE together with ICBAS/UP coordinate the PROBIOSOLEA project in consortium with a Portuguese company Biostrument. (Project QREN 5362). The aim of PROBIOSOLEA is to minimize the incidence of diseases related disorders in sole by use of probiotics. To improve the intestinal balance, immunologic defenses, thus, the well-being of the animals by applying “blue aquaculture” practices.


LANUCE has long established collaborations with several international Institutes to develop research programs and co-supervision of PhD students:

- SEACASE, financed by the EU, involving 5 Countries, coordinated by CCMAR.

- NIFES, Bergen, Norway. Co-supervision of 1 PhD thesis (Marit Espe): 3 publications.

- UMR, Nutrition Aquaculture Génomique, INRA, St-Pee-Sur Nivelle, France. Co-supervision of a PhD thesis by Geneviéve Corraze: 4 publications. 1 COST action 825.

- Bødo University, Norway. Co-supervision of a PhD thesis by Jorge Fernandes. 1 COST action 825, 1 proposal for a PhD thesis.

- NIOZ; Royal Netherlands Institute for Sea Research, The Netherlands. Co-supervision of 1 MSc thesis (CC de Aguiar Carvalho).

- Barcelona University, Spain (Joaquin Gutierrez). 2 publications. 1 COST action 825.

- Instituto Español de Oceanografia and Consellería de Pesca e Asuntos Marítimos (CPAM), Spain (Peleteiro, T, Olmedo, M, Linares, F, Villanueva, JLR). 1 Interreg project submitted and 1 Spanish project. 2 publications.

- University of Santiago de Compostela (USC), Spain. Co-supervision of 1 PhD thesis (Manuel Rey Mèndez and Ana Otero). 4 publications.

- Through an IBEROEKA network (IBK 10-633), CIIMAR has international collaboration with the State University of São Paulo (UNESP, Brazil) and Federal University of Pernambuco (UFRPE, Brazil). Co-supervision of 1 PhD thesis and 3 MSc Thesis.

- Through the Edital Ciências do Mar Nº. 09/2009 (CAPES, Brazil), CIIMAR has an international collaboration with the Federal University of São Paulo (ESALQ-USP, Brazil), Centro de Energia Nuclear Aplicada a Agricultura (CENA-USP, Brazil), and Agencia Paulista de Tecnologia dos Agronegócios (APTA).

- COST Action FA0801 - Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network (LARVANET).



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Oceanic and Coastal Dynamics



Oceanic and Coastal Dynamics


Maria Luisa Machado Cerqueira Bastos






PTDC/AAC-CLI/105296/2008, Long Term Variability of the Canary Current Upwelling System - LongUp. CIIMAR: 81.454 EUR. 2010-2013.

PTDC/ECM/103801/2008, MOWADI. CIIMAR: 6.600 EUR, 2010-2012.

PTDC/AAC-AMB/113-469/2009, PACMAN. CIIMAR: 23.904 EUR.

PTDC/MAR/108177/2008, The Azores and St. Helena currents from satellite altimetry: assessing their congeneracy and role in the subtropical Atlantic (ASH). CIIMAR: 54.260 EUR.

ERA-NET AMPERA - FCT, DEOSOM - Detection and Evaluation of Oil Spills by Optical Methods.. CIIMAR: 43.800 EUR, 2008-2011.


INTERREG IV POCTEP, RAIA - Observatorio Oceánico del Margen Ibérico. CIIMAR: 121.651 EUR, 2009-2011.

8.770 EUR, GEOMAD and i-WAKE, EUFAR Transnational access projects.

8.400 EUR, ESA/ESRIN, Development of Radar Altimetry data processing in the oceanic coastal zone (COASTALT – Phase 2).

CNES/ISRO, SARAL/AltiKa AO, Characterization of the subtropical Atlantic from the synergistic use of satellite altimetry and remote sensing data (AtlantiKa). No funding.


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ESA, AO for GOCE 4319, Coastal Altimetry Improvement in the Portuguese EEZ for Geodesy and Oceanography – CALTIGO. No funding.

EUMETSAT/CNES, CNES-DSP/OT/07-4571, Coastal ALtimetry - Improvement of the wet TROpospheric correction (CALITRO). No funding.


1. Objectives

Main objectives were related to the study of ocean, coastal and estuarine dynamics, focused on the following aspects:

- Evaluation and understanding of long term sea-level variations along the Portuguese coast, achieved through the analysis of historical and actual tide gauge data, including reliability assessment. GPS measurements at tide gauge were also analysed to monitor and understand the vertical land movement at these locations.

- Research of tidal phenomena such as ocean tides in front of the Portuguese coast and ocean tide loading in the Canary Islands.

- Research into Madeira Island wakes, using numeric and laboratory models, satellite remote sensing data, and in situ observations.

- Investigation of the long term variability of the Canary Current Upwelling System (CCUS), to assess how the structure of the CCUS responds, in decadal scales, to the global warming.

- Investigation of the influence of the mesoscale activity on the coastal ecosystem functioning, to assess how coastal ocean dynamics controls the distribution of chemical and biological properties and their cross-shore transport.

- Study of the generation, propagation and dissipation of internal solitary waves (ISW) in coastal ocean and deep ocean environments.

- Exploitation of satellite remote sensing data, for different applications and studies such as: development of methods for coastal altimetry, namely the development of new methodologies for the computation of the wet tropospheric correction to improve coastal altimetry; exploitation of satellite radar imagery as useful information on past and future oil spill disasters; support to numerical model runs, providing initial conditions and validation data.

- Exploitation of satellite positioning techniques (with real-time direct georreferencing capabilities) integrated with laser systems for the implementation of a waterborne system for water pollution surveillance.


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- Understanding of coastal and estuarine morphodynamics, through environmental monitoring using: terrestrial mobile mapping techniques (with videogrametry); waterborne bathymetry and bottom morphology monitoring (with sonar equipment); and, airborne photogrammetric determination of terrain topography.

- Assessment of the usefulness of high resolution SAR image data for studies of coastal erosion processes, their evolution and climate impacts.

Further objectives in 2010 were:

- Networking and integration of the COD team within the international and coastal and oceanographic research communities.

- Improvement of the high performance computing system (HPC) of CIIMAR to support the different numerical modeling studies.

- Implementation of a COD dedicated web page for dissemination of the group activities.

- Training of MSc and PhD students, both nationally and internationally, including collaboration in doctoral programmes.

- Preparation and submission of applications to national and international funding schemes, including FCT, INTERRREG and FP7 projects.

- Dissemination and knowledge transfer in national and international meetings.

- Participation in the LA initiatives for the dissemination of scientific results and public outreach.

- Cooperation with other CIIMAR research groups through the support in the use, analysis and exploitation of georeferenced and remote sensing data.

- To provide oceanic and meteorological forecasts (in cooperation with other researchers) and release the information through the COD web page.

- Maintain collaboration with the Lisbon Oceanography Institute in the study of atmospheric gravity waves in the Red Sea.


Tides and sea-level studies:

- Preservation, processing and analysis of historical sea level records from the Leixões tide gauge covering the period 1890–2004. Records were digitised and hourly sea levels extracted.

- Development of a tide model for several Portuguese coastlines. Examples include a regional tide model for the West Iberian Margin developed in the scope of the COASTALT project and a tide model for the Selvagens developed for the


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EMEPC/M@rbis/Selvagens 2010 campaign. Recognition of the work has generated new requests for models in the Kattegatt area (Sweden) and Loch Ness (United Kingdom).

- Reanalysis of the tide gauge data from Cascais using a methodology developed by COD. Sea-level rise at this station was estimated using a new noise model, to obtain realistic errors for the estimated sea-level rise.

- An innovative method for the computation of the wet tropospheric correction for satellite Coastal Altimetry measurements, was developed and implemented in the SW European region. The method is based on the combination of GNSS-derived tropospheric delays with valid Microwave Radiometer (MWR) measurements and numerical weather model (NWM) data. This study is being extended to reach the maturity required for an operational and desirable global implementation.

Ocean and atmospheric processes at Madeira.

- One of the challenges faced in the study of Madeira Island wakes has been the lack of sampling means to obtain a synoptic (i.e., quasi-simultaneous) high-resolution view of atmospheric and oceanic wakes. The I-WAKE project, allowed for collection of airborne observations of atmospheric wakes (high-altitude) and of oceanic wakes (low-altitude), as well as concurrent oceanographic data.

- To improve the height reference surface around Madeira region, an airborne gravimetry campaign, 'GEOMAD', took place together with the I-Wake campaign. The results will provide a new local geoid for Madeira and together with satellite altimetry, have the potential to give information on the surface currents around Madeira.

- COD participated in EMEPC/M@rbis/Selvagens 2010 campaign, with the objective of overseeing the physical oceanography aspects of this mission. In-situ data (transects) were collected during a wake episode south of Madeira.

- Assessment of the dynamic nature (temporal and spatial scales) of (sub) meso-scale island-induced features around the Madeira Archipelago, was the aim of ESA project (6248) focussing mostly on the synergy between Synthetic Aperture Radar data and Ocean color.

Ocean circulation:

- Preliminary results on the long term variability of the CCUS showed that in the Iberian sector different mesoscale features respond to the observed global warming in different ways. During 2010 it was possible to extend the research to the entire CCUS from 43 to 10ºN.

- Quantification of the cross-shelf exchange of chemical and biological properties between coastal and oceanic waters off SW Iberia; conclusions about the impact on the regional oceanography. The investigation of the inner shelf dynamics and its control on biological properties was initiated.


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- Gridded maps of zonal (U) and meridional (V) components of the surface geostrophic currents have been derived. Sea Level Anomaly (SLA) grids were derived for the period June 1995 to present, using a linear space-time objective analysis technique that combines multi-mission along-track SLA values referred to the GSFC00.1 Based on the SLA grids, Absolute Dynamic Topography (ADT) grids have been computed, supported by historical hydrographic data.

- Analysis of a comprehensive dataset of ENVISAT ASAR and TerraSAR-X imagery of the central Mascarene Plateau (Indian Ocean), which showed packets of large ISWs propagating towards the west-northwest and the east-southeast from the sill between Saya de Malha and Nazareth Banks near 12.51S, revealed the largest waves yet recorded anywhere in the world’s ocean. New insights into the generation mechanisms of internal waves, recently observed in the Indian Ocean, were obtained.

Coastal processes studies:

- Assessment of present coastal dynamics through coastal monitoring of land forms and processes, and their forcing factors for vulnerability mapping and risk assessment. Erosion risk evaluation on specific coastal sectors and risk maps were based on comprehensive monitoring program results, hydro-dynamic models and meteorological data.

- Development of Matlab code for a k-means type algorithm for automatic detection of oil spills in SAR images. The algorithm will be adapted to detect the coastline morphology in aerial photographs and high resolution TerraSAR-X images.

- Collaboration in the development of a low cost laser based system for early oil spill detection in riverine and coastal waters.

8d. Productivity


1. Bos, MS. 2010. Comment on “Anomalous ocean load tide signal observed in lake-level variations in Tierra del Fuego” by A. Richter et al. Geophysical Research Letters 36: L04303.

2. Bos, MS, Bastos, L, Fernandes, RMS. 2010. The influence of seasonal signals on the estimation of the tectonic motion in short continuous GPS time-series. Journal of Geodynamics 49(3-4): 205-209.

3. Campos, J, Bio, A, Cardoso, JFMF, Dapper, R, Witte, J IJ, Van der Veer, HW. 2010. Fluctuations of brown shrimp Crangon crangon abundance in the western Dutch Wadden Sea. Marine Ecology Progress Series 405: 203-219.

4. Cravo, A, Relvas, P, Cardeira, S, Rita, F, Madureira, M, Sánchez, R. 2010. An upwelling filament off Southwest Iberia: effect on the chlorophyll a and nutrients exportation, Continental Shelf Research 30: 1601-1613.


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5. Fernandes, MJ, Lázaro, C, Nunes, AL, Pires, N, Bastos, L, Mendes, VB. 2010. GNSS-derived Path Delay: an approach to compute the wet tropospheric correction for coastal altimetry, IEEE Geoscience and Remote Sensing Letters 7(3): 596-600.

6. Granja, H, Rocha, F, Matias, M, Moura, R, Caldas, F, Marques, J, Tareco, H. 2010. Lagoa da Apúlia: a residual lagoon from Late Holocene (NW coastal zone of Portugal). Quaternary International, 221: 46-57.

7. Honrado, J, Vicente, J, Lomba, A, Alves, P, Macedo, JA, Henriques, R, Granja, H, Caldas, FB. 2010. Fine-scale patterns of vegetation assembly in the monitoring of changes in coastal sand-dune landscapes. Web Ecology 10: 1-14.

8. King, MA, Altamimi, Z, Boehm, J, Bos, M, Dach, R, Elosegui, P, Fund, F, Hernandez-Pajares, M, Lavallee, D, Cerveira, PJM, Penna, N, Riva, REM, Steigenberger, P, van Dam, T, Vittuari, L, Williams, S, Willis, P. 2010. Improved constraints on models of glacial isostatic adjustment: A review of the contribution of ground-based geodetic observations. Surveys in Geophysics 31(5): 465-507.

9. Macedo, JA, Alves, P, Lomba, A, Vicente, J, Hespanhol, H, Henriques, R, Granja, H, Honrado, J. 2010. On the interest of plant functional classifications to study community-level effects of increased disturbance on coastal dune vegetation. Acta Botanica Gallica 157 (2): 305-315.

10. Madeira, S, Gonçalves, JA, Bastos, L. 2010. Photogrammetric mapping and measuring application using MATLAB. Computers and Geosciences 36(6): 699-706.

11. Sousa, JJ, Ruiz, AM, Hanssen, RF, Bastos, L, Gil, AJ, Galindo-Zaldívar, J. 2010. PS-InSAR processing methodologies in the detection of field surface deformation – study of the Granada basin (Central Betic Cordilleras, Southern Spain). Journal of Geodynamics 49: 181-189.

In press

12. Geng, Y,Deurloo, R, Bastos, L. (in press). Hybrid Derivative-free Extended Kalman Filter for Unknown Lever-arm Estimation in Tightly-coupled DGPS/INS Integration. GPS Solutions, doi: 10.1007/s10291-010-0190-8.


1. Bastos, L, Bos, MS, Fernandes, RMS. 2010. Deformation and Tectonics: Contribution of GPS Measurements to Plate Tectonics – Overview and Recent Developments, In: Xu, Guochang (Ed).

Sciences of Geodesy - I, Advances and Future Directions, pp. 155184. ISBN 978-3-642-11740.

2. Bio, A, Meirinho, A, Ramírez, I, Geraldes, P. 2010. Empirical modelling of Balearic shearwater (Puffinus mauretanicus) census data for the Portuguese Atlantic. In: Calado, H, Gil, A (Eds). Geographic technologies applied to Marine Spatial Planning and Integrated Coastal Zone Management, Centro de Informação Geográfica e Planeamento Territorial, Azores University, pp. 23-30. ISBN: 978-972-8612-64-1.


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3. Granja, HM. 2010. Les dunes côtières du nord-ouest du Portugal. In: Roland Paskoff et les littoraux: regards de chercheurs. In: Duvat, V, Battiau-Queney, Y, Clus-Auby, C, Prat, M-C (Eds). L’ Harmattan, Paris, pp. 230-238. ISBN:978-2-296-13264-1.

4. Granja, HM. 2010. Le combat de Roland Paskoff contre la defense lourde des côtes. In: Roland Paskoff et les littoraux: regards de chercheurs. Duvat, V, Battiau-Queney, Y, Clus-Auby, C, Prat, M-C (Eds). L’ Harmattan, Paris, pp. 277-287. ISBN:978-2-296-13264-1.

5. Robinson, IS, da Silva, J. 2010. Chapter 12 Internal waves. In: Robinson, IS (Ed). Discovering the Ocean from Space, The unique applications of satellite oceanography. Springer Verlag Berlin Heidelberg, pp. 453-483. ISBN: 978-3-540-24430-1.

6. Araújo, IB, Caldeira, R, Couvelard, X. 2010. Island induced (sub) mesoscale features around Madeira Archipelago (initial findings). Proceedings of the Third International Workshop SeaSAR 2010 ‘Advances in SAR Oceanography from ENVISAT, ERS and ESA third party missions’. 25-29 January, Frascati, Italy. ESA SP-679, CD-ROM, 8p.

7. Gonçalves, H, Gonçalves, JA, Corte-Real, L. 2010. The use of similarity images on multi-sensor automatic image registration. In: Wagner, W, Székely, B (Eds): ISPRS TC VII Symposium – 100 Years ISPRS, 5-7 July 2010, Vienna, Austria. IAPRS, Vol. XXXVIII, Part 7B, pp. 233-238.

8. Gonçalves, JA. 2010. Automatic image orientation and DSM extraction from ALOS-PRISM triplet images. Proceedings of the 2010 IEEE International Geoscience and Remote Sensing (IGARSS'10), pp. 2295-2298.

9. Gonçalves, JA, Bastos, L, Perez, B, Magalhães, A. 2010. Monitoring of beaches and sand dunes using digital aerial photography with direct georeferencing. In: Wagner, W, Székely, B. (Eds): ISPRS TC VII Symposium – 100 Years ISPRS, 5-7 July 2010, Vienna, Austria, IAPRS, Vol. XXXVIII, Part 7B, pp. 228-232.

10. Lazar, A, Stegner, A, Caldeira, R, Pennel, R, Dong, C, Didelle, H, Viboud, S. 2010. Vortex Assymetry in island wakes. Proceedings of the HYDRALAB III Joint User Meeting, 1-4 February 2010, Hannover, Germany, 4p. ISBN: 978-3-00-030141-4.


1. Barbosa, SM, Fernandes, MJ. 2010. Variação do nível do mar em Cascais: Sazonalidade & tendência. Actas da VI Conferência Nacional de Cartografia e Geodesia, Centro Cultural de Congressos das Caldas da Rainha, Edições Lidel, pp. 474-481.

2. Gonçalves, J, 2010. Conversões de Sistemas de Coordenadas Nacionais para ETRS89 Utilizando Grelhas. Cartografia e Geodesia 2009, Edições Lidel, Lisboa.

3. Pires, N, Fernandes, MJ, Nunes, A. 2010. Viabilidade da aplicação dos produtos troposféricos IGS e EPN na altimetria por satélite em zonas costeiras. Actas da VI Conferência Nacional de Cartografia e Geodesia, Centro Cultural de Congressos das Caldas da Rainha, Edições Lidel, 378-390.


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MASTER THESES

Faria, Rosa. 2010. Optimização do processamento de dados GNSS de alta taxa de amostragem em tempo quase-real em modo singular Master thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: Rui Fernandes, Co-supervisor: José A Gonçalves.

Mendes, Ricardo. 2010. Orientação e calibração de dados ALS. Master thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: Ismael Colomina, Co-supervisor: José A Gonçalves.

Nascimento, Ângela. 2010. Campanha MPDO para sardinha (IPIMAR, 2005). Caracterização hidrográfica sobre a plataforma continental Ibérica. Aplicação dos dados de perfis de temperatura à datação de ovos de sardinha, efeitos sobre a estimativa de produção de ovos. Master thesis in Oceanography, Faculty of Sciences and Technology, University of Algarve. Supervisor: Paulo Relvas, Co-supervisor: Maria Manuel Angélico (INRB-IPIMAR).

Vasco, Enoque Canganjo. 2010. Caracterização hidrográfica e química da região costeira de Benguela, Angola. Master thesis in Oceanography, Faculty of Sciences and Technology, University of Algarve. Supervisor: Paulo Relvas, Co-supervisor: Alexandra Cravo.

PhD THESES

Gonçalves, Hernâni. 2010. Automatic registration of satellite images. PhD thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: José A Gonçalves (CIMAR-OCD), Co-supervisor: Luís Corte-Real (INESC Porto, Faculty of Engineering, University of Porto).

Sousa, Joaquim, 2010. Integration of DInSAR and DGPS for location and characterization of active faults. Supervisor: Luísa Bastos, Co-supervisor: Ramon Hanssen (Delft University of Technology, The Netherlands).

Azevedo, Alberto Carlos de Oliveira Antunes. 2010. Sistema integrado de modelação para apoio e mitigação de acidentes de hidrocarbonetos em estuários e orla costeira. PhD thesis, LNEC/University of Lisbon. Supervisors: Anabela Pacheco de Oliveira (LNEC), José CPB Teixeira da Silva.



4th Workshop on Coastal Altimetry, 14-15 October 2010, Faculty of Sciences, University of Porto, Porto, Portugal. Joana Fernandes - Chair of the Organizing Committee and member of the Local Committee; Clara Lázaro - Member of the Scientific and Local Committees; Alexandra Nunes - Chair of the Local Committee.


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Workshop “Sea-level rise along the Portuguese coast”, 28 May 2010, CIIMAR, Porto. Isabel Araújo and Machiel Bos – Members of the Organizing and Scientific Committees.

15th General Assembly of WEGENER Istanbul, September 14-17 2010, Turkey. Luísa Bastos - Member of Scientific Committee.


“Conservação e Análise do Registo Histórico dos Maregramas de Leixões” under contract with the Administração dos Portos do Douro e Leixões (APDL, Port Authority) to analyse more than 100 years of tide gauge data from Leixões.


Collaboration with several international research centers that resulted in the submission of joint publications and projects.

Instituto de Astronomía y Geodesia, Spain, in the analysis of tidal gravity observations made at the Canary Islands, to study the elastic properties of the Earth in that region. The results will be published in the article “Verifying the body tide at the Canary Islands using tidal gravimetry observations”, accepted for publication (in 2011) by the Journal of Geodynamics.

- Onsala Space Observatory, Sweden, in ocean tide loading. Writing of a chapter on ocean tide loading that will be submitted to the book editor by April 2011.

- The University of Newcastle, United Kingdom, in the project "Global loading and deformation at tidal timescales". This project will validate the ocean tides with GPS observations.

- University of Vienna, CLS-Soprano, Laboratoire de Meteorologie Dynamique and CNRS from France, in the submission of joint projects for the study of ocean and atmospheric processes in Madeira.

- The group was involved in an international proposal led by the National Oceanographic Centre, Southampton (UK) in the scope of the ESA Climate Change Initiative, aiming at the generation of sea level products for climate studies.

- Meteogalicia, Centro Tecnológico del Mar, Instituto Tecnolóxico para o Control do Medio Mariño, Instituto Español de Oceanografía, Instituto de Investigaciones Marinas in the preparation of the project Observatorio Marino del Margen Ibérico y del Litoral.

- Biology Department, Woods Hole Oceanographic Institution, and University of Princeton, in the USA; Ocean Modelling and Forecasting Group, National Oceanography Centre and University of Loughborough in the U.K., resulting in joint publications.


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Development of Radar Altimetry data processing in the oceanic coastal zone (COASTALT – Phase 2), ESA/ESRIN Contract No. 21201/08/I-LG (CCN3), funded by ESA, period 2008-2011. Results from the modeling task were reported to ESA.

Airborne observations of Nonlinear Evolution of internal Waves generated by internal (tidal) beams, a new EUFAR EU Project; participating institutions and project leaders: University of Lisbon, JCB da Silva, T. Gerkema, NIOZ Holland, L. Gostiaux, Coriolis Lab, France, A.L. New, National Oceanography Centre, Southampton, UK; research aircraft time (no funding involved).

The generation of internal tides and solitary waves by submarine canyons and plateaus off the Portugal coast, Treaty of Windsor CRUP Project; participating institutions and project leaders: Loughborough University, Prof Roger Grimshaw and University of Lisbon, Prof José da Silva.


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Pathology



Pathology


Jorge Guimaraes da Costa Eiras




FCT Pluriannual funding.

4.000 EUR, Sistema de Incentivos à I&DT - FEDER - Quadro de Referência Estratégico Nacional 2007-2013, OPTISOLE - Investigação e desenvolvimento na produção de juvenis de linguado: incremento da resistência a patologias e do crescimento.

1.428 EUR, Scientific Illustration Colour Courses 1st Edition and 2 Edition and Nature Photography .

672 EUR, FCT BII grants CIIMAR/C2008/BII-11/2010.


1. Objectives

The Laboratory of Pathology is a laboratory dedicated to the Pathology of Aquatic Organisms, principally Fish. The main research lines of the Laboratory are: 1) Fish bacteriology; 2) Pathology of farmed fish; 3) Fish parasitology; 4) Impact of fish parasites in Public Health; 5) Ecology of fish parasites.

The specific objectives for the next year / years are:

- Contribute to a better knowledge of fish, molluscs and annelids parasites specially that ones with greater impact in fisheries, aquaculture and public health;

- Evaluate the use of parasites as biological tags for commercially important fish species;

- Evaluate the impact of Kudoa (Myxozoa) infection in sardine flesh quality


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- Continue the studies on parasite species that infect several hosts collected in different shallow-water ecosystems;

- Continue the studies on bacterial fish diseases specially that ones important to Fish Farms;

- Continue the studies on the detection and isolation of bacteriophages from the environment and their potential use as prophylactic and therapeutic agents against fish pathogenic bacteria.


- Description of several new species of Myxozoa and Microsporea from fishes from Africa and South America using electron microscopy and, or, molecular approach.

- Description of species of Myxozoa (Actinosporea) of Polychaetes.

- Preliminary results of Kudoa (Myxozoa) infection levels on Portuguese sardine.

- Preliminary results on the pathology of farmed turbot (Psetta maxima).

- The study of the populational dynamic of flounder and octopus parasites.

- First results on the parasites of blackspot seabream Pagellus bogaraveo, a highly appreciated fish with great commercial value and recently introduced in aquaculture.

- Description of a new species, Caligus musaicus 2010 Cavaleiro, Santos & Ho (Copepoda: Caligidae) parasitic on the flounder Platichthys flesus (Linnaeus) off Portugal.

- Description of a new species- Halomonhystera parasitica 2010 Poinar, Duarte & Santos (Nematoda: Monhysteridae), a parasite of Talorchestia brito (Crustacea: Talitridae) in Portugal.

- The results obtained allowed the publication of several papers (16) in International Journals referred (ISI) and the presentation of communications in international (12) and national (16) meetings.

- 4 PhD, 6 MSc and 3 undergraduate students performed their research in the Laboratory and 1PhD and 6 MSc theses were completed with success.

- The “XIV Congresso Português de Parasitologia” (Annual Portuguese Meeting of Parasitology),which took place in the Faculty of Sciences, University of Porto (8-10 September) was organized by some team members (JC Eiras, A Saraiva, C Cruz, M João Santos). It was attended by 102 people - 38 oral communications and 70 poster communications were presented, besides the organization of some round tables on important parasitological topics.


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8d. Productivity


1. Azevedo, C, Marques, DKS, Casal, G, Amaral, CMC, Silva, EV, Matos, P, Matos, E. 2010. Ultrastructural re-description of Henneguya piaractus (Myxozoa), a parasite of the freshwater fish Piaractus mesopotamicus (Teleostei, Characidae) from the Paraguay river, Brazil. Acta Protozoologica 49: 115-120.

2. Azevedo, C, Casal, G, Mendonça, I, Carvalho, E, Matos, P, Matos, E. 2010. Light and electron microscopy of Myxobolus sciades n. sp. (Myxozoa), a parasite of the gills of the Brazilian fish Sciades herzbergii (Block, 1794) (Teleostei: Ariidae). Memórias Instituto Oswaldo Cruz 105(2): 203-207.

3. Azevedo, C, Samuel, N, Saveia, A, Delgado, F, Casal, G. 2010. Light and electron microscopic data of spore of Thelohanellus rhabdalestus n. sp. (Myxozoa: Myxosporea), a parasite of a freshwater fish from Kwanza River of Angola. Systematic Parasitology, 79: 19-25.

4. Casal, G, Teles-Grilo, ML, Matos, E, Azevedo, C. 2010. Ultrastructural and molecular characterization of a new Microsporidium parasite from the Amazonian fish, Gymnorhamphichthys rondoni (Rhamphichthyidae). Journal of Parasitology 96: 1155-1163.

5. Cavaleiro, FI, Santos, MJ, Ho, J-S. 2010. Caligus musaicus n. sp. (Copepoda: Caligidae) parasitic on the flounder Platichthys flesus (Linnaeus) off Portugal. Crustaceana 83 (4): 457-464.

6. Eiras, JC, Monteiro, CM, Brasil-Sato, MC. 2010. Myxobolus franciscoi sp. nov. (Myxozoa, Myxobolidae), a parasite of Prochilodus argenteus (Actynopterygii, Prochilodontidae) from the Upper São Francisco River, Brazil, with a revision of Myxobolus spp. from South America. Zoologia 27: 131-137.

7. Francisco, C, Almeida, A, Castro, A, Santos, MJ. 2010. Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus. Parasitology International 59: 40-34.

8. Francisco, C, Hermida, M, Santos, MJ. 2010. Parasites and Symbionts from Mytilus galloprovincialis (Lamark, 1819) (Bivalves: Mytilidae) of the Aveiro Estuary Portugal. Journal of Parasitology. 96(1): 200-205.

9. Marques, JF, Santos, MJ, Cabral, HN. 2010. Aggregation patterns of macroendoparasites in phylogenetically related fish hosts. Parasitology 137(11): 1671-1680.

10. Milanin, T, Eiras, JC, Arana, S, Maia, AAM, Alves, AL, Silva, MRM, Carriero, MM, Ceccarelli, PS, Adriano, EA. 2010. Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai n. sp., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil. Memórias Instituto Oswaldo Cruz 105: 762-769.

11. Poinar, G, Duarte, D, Santos, MJ. 2010. Halomonhystera parasitica n. sp. (Nematoda: Monhysteridae), a parasite of Talorchestia brito (Crustacea: Talitridae) in Portugal Systematic Parasitology 75: 53-58.


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12. Romano, L, Eiras, JC, Raibenberg, FC, Alvares, M, Saraiva, A, Cruz, C. 2010. A case of ceroid deposition in the kidney of feral rainbow trout, Oncorhynchus mykiss, in Alicura impoundment, Argentina. Bulletin of the European Association of Fish Pathologists 30: 25-29.

13. Santos, MJ, Cavaleiro, F, Campos, P, Sousa, A, Teixeira, F, Martins, M. 2010. Impact of amoeba and scuticociliatidia infections on the aquaculture European sea bass (Dicentrarchus labrax L.) in Portugal. Veterinary Parasitology 171: 15-21.

14. Swiderski, Z, Gibson, DI, Santos, MJ, Poddubnaya, LG. 2010. Ultrastructure of the intrauterine eggs of Didymobothrium rudolphii (Monticelli, 1890) (Cestoda, Spathebothriidea, Acrobothriidae) and its phylogenetic implications. Acta Parasitologica 55(3): 254-269.

In press

15. Francisco, C, Almeida, A, Castro, A, Pina, S, Fernanda, R-P, Pedro, R, Santos, MJ. (in press). Morphological and molecular analysis of metacercariae of Diphtherostomum brusinae (Stossich, 1888) Stossich, 1903 from a new bivalve host Mytilus galloprovincialis. Journal of Helminthology, doi: 10.1017/S0022149X10000428.

16. Marques, J, Santos, MJ, Teixeira, CM, Batista, MI, Cabral, HN. (in press). Host-parasite relationships in flatfish (Pleuronectiformes) – the relative importance of host biology, ecology and phylogeny. Parasitology, doi: 10.1017/S0031182010001009.


1. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2010. Diversidade dos Parasitas de Peixes de Água Doce do Brasil. Maringá, Brasil, Clichetec, 333 pp.


1. Castro, R, Silva, A, Casal, G, Cavaleiro, FI, Santos, MJ. 2010. Estudo comparativo dos ectoparasitas da cavala, Scomber japonicus Houttuyn, 1782 e da sarda, Scomber scombrus Linnaeus, 1758 na costa norte de Portugal Continental. Acta Parasitológica Portuguesa 17(2): 85.

2. Cavaleiro, FI, Santos, MJ. 2010. Análise microambiental da infecção de Octopus vulgaris Cuvier, 1797 (Cephalopoda: Octopodidae) por Octopicola superba superba Humes, 1957 (Copepoda: Octopicolidae) na costa Portuguesa. Acta Parasitológica Portuguesa 17(2): 92.

3. Cavaleiro, FI, Santos, MJ. 2010. Padrão de ocorrência espacial de Octopicola superba superba Humes, 1957 (Copepoda: Octopicolidae) em polvo comum, Octopus vulgaris Cuvier, 1797 (Cephalopoda: Octopodidae), da costa Portuguesa. Acta Parasitológica Portuguesa 17(2): 91.

4. Correia, M, Cavaleiro, FI, Formigo, NE, Santos, MJ. 2010. Restrição de nichos ecológicos no tracto intestinal da solha Europeia Platichthys flesus (Linnaeus, 1758) (Teleostei: Pleuronectidae) por helmintas parasitas. Acta Parasitológica Portuguesa 17(2): 62.


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5. Francisco, CJ, Almeida, A, Castro, AM, Pina, S, Russel-Pinto, F, Rodrigues, P, Santos, MJ. 2010. Variabilidade da região ITS1 de Diphtherostomum brusinae (Trematoda: Zoogonidae). Acta Parasitológica Portuguesa 17(2): 129.

6. Francisco, CJ, Bray, RA, Lanfranchi, AL, Almeida, A, Timi, JT, Castro, AM, Santos, MJ. 2010. Caracterização molecular de Prosorhynchus spp. (Trematoda: Bucephalidae). Acta Parasitológica Portuguesa 17(2): 45.

7. Pina, S, Russell-Pinto, F, Rodrigues P. 2010. Caracterização morfológica e molecular da metacercária Microphallus primas (Digenea: Microphallidae). Acta Parasitológica Portuguesa 17(2): 44.

8. Rangel, LF, Santos, MJ. 2010. Um novo método para a amostragem de parasitas Myxozoa no celoma de poliquetas marinhos. Acta Parasitológica Portuguesa 17(2): 83.

9. Santos, MJ, Severino, R. 2010. Métodos de controlo de Amyloodinium ocellatum em robalo (Dicentrarchus labrax L.) “amigos” do ambiente. Acta Parasitológica Portuguesa 17(2): 60.

10. Severino, R, Santos, MJ. 2010. Controlo químico do ectoparasita Amyloodinium ocellatum no robalo (Dicentrarchus labrax). Acta Parasitológica Portuguesa 17(2): 87.

11. Silva, A, Castro, R, Cavaleiro, FI, Santos, MJ. 2010. Estudo comparativo dos endoparasitas da cavala, Scomber japonicus Houttuyn 1782, e da sarda, Scomber scombrus, Linnaeus 1758, da costa Noroeste de Portugal. Acta Parasitológica Portuguesa 17(2): 84.


MASTER THESES

Barbosa, Ana. 2010. Estudo de parâmetros hematológicos de pregado (Psetta maxima Linnaeus, 1758) de cultura Master thesis in Biological Aquatic Resources, Faculty of Sciences, University of Porto. Supervisor: Cristina Cruz.

Jerónimo, Daniel Baptista. 2010. Pesquisa de agentes patogénicos em rodovalho (Psetta maxima L.) de cultura. Master thesis in Aquatic Biologic Resources, Faculty of Sciences, University of Porto. Supervisor: Aurélia Saraiva.

Marques, Joana Filipa. 2010. Caracterização bioquímica e molecular de estirpes de Yersinia ruckeri isoladas em Portugal. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: José Américo Sousa.

Mendes, Cristiane. 2010. Caracterização de isolados de Vibrio anguillarum originários de pisciculturas portuguesas. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: José Américo Sousa.

Reisinho, Inês Isabel Ribeiro Oliveira. 2010. Pesquisa de parasitas de tainha do estuário do rio Douro. Master thesis in Sciences of the Sea - Sea Resources, ICBAS, University of Porto. Supervisor: Aurélia Saraiva.


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Silva, Ana. 2010. Contribuição para o estudo da parasitose provocada por Kudoa sp. em Sardina pilchardus (Walb., 1792.) Master thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Cristina Cruz.

PhD THESES

Francisco, Claire Juliana. 2010. Parasitas do mexilhão Mytilus galloprovincialis Lamark, 1819 (BIVALVIA: MYTILIDAE) com relevância para Trematoda: ciclo de vida, características morfológicas e moleculares. PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Maria João Santos.



XIV Congresso Português de Parasitologia, Faculty of Sciences, University of Porto, 8-10 September 2010. JC Eiras - President, A Saraiva, C Cruz, M João Santos – Members of the Organizing Committee.


- Project OPTISOLE - Investigação e desenvolvimento na produção de juvenis de linguado: incremento da resistência a patologias e do crescimento - project funded under “Sistema de Incentivos à I&DT - FEDER - QREN”.

- Technical assistance to fish farms.


Collaboration with institutions from different countries:

- Univ. Kingston, UK (Prof. Angela Davis).

- Natural History Museum, London, UK (Prof. David Gibson, Dr. Rod Bray).

- Czech Academy of Sciences, Czech Republic (Prof. F Moravec, Prof. T Scholz).

- Hungarian Academy of Science, Hungary (Prof. K Molnár, Prof. C Szekély).

- Univ. Santiago de Compostela, Fac. Biologia, Dep. Microbilogia y Parasitologia, Spain (Prof. Alicia Toranzo, Prof. Juan Barja).

- Instituto de Investigaciones Marinas, Vigo, Spain (Dr. António Figueras, Dr. Camiño Gesta).

- Centro de Control do Medio Mariño (CCMM), Consellería de Pesca e Asuntos Marítimos, Spain (Dr. Jaime Montes).


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- Instituto Nacional de Investigação Pesqueira de Luanda, Angola (Ana Paula, Nelson Samuel).

- California State Univ. Long Beach, USA (Prof. Jo-shey Ho).

- Oregon State Univ., Depart. Zoology, Corvallis, USA (Prof. George Poinar)

- Humboldt State Univ., California, USA (Dr. Katie R. McGourty).

- Univ. Maringá, Brazil (Prof. Gilberto Pavanelli, Prof. Ricardo Takemoto).

- Univ. Rio Grande, Brazil (Prof. Joaber).

- Fisheries Institute of São Paulo, Brazil (Prof. Maria José Ranzani).

- Carlos Azevedo Research Laboratory, Federal Rural University of Amazónia, Belém, Brazil (Prof. Dr. Edilson Matos).

- Federal Univ. Pará, Belém, Brazil (Prof. Patrícia Matos).

- Federal Univ. Pernambuco, Recife, Brazil (Prof. Dr. Paulo Padovan, Prof. Dr. Isaíras Padovan).

- Federal Univ. Piauí, Fac. Med. Veterinária,Teresina, Brazil (Prof. Ivete Mendonça).

- Federal Univ. Santa Catarina, Florianópolis, Brazil (Prof. Patrícia Garcia).

- Univ. Federal Fluminense, Fac. Med. Veterinária,Niterói, Brazil (Prof. Dr. Sérgio Carmona, Prof. Dr. Marcelo Knoff).

- Univ. Federal Rural do Rio de Janeiro (Prof. Dr. José Luis Luque).

- Enbrapa, Corumbá, Mato Grosso do Sul, Brazil (Dr. Débora Marques).

- Univ. Nacional de Mar del Plata, Facultad de Ciencias Exactas y Naturales, Departamento de Biología, Laboratorio de Parasitología, Mar del Plata, Argentina (Prof. Juan Timi).

Collaboration in the project “Revisión de la familia Gymnophallidae Odhner, 1900 basada en estudios morfológicos y moleculares” with Florencia Cremonte, Centro Nacional Patagónico, Provincia del Chubut, Argentina.

Evaluation of PhD thesis “Studies on Monogenetic Trematodes of some fishes of Karachi coast” of the Jinnah Univ. for Women, Nazimabad, Karachi, Pakistan (MJ Santos).

Portuguese contact of the International Ichthyoparasitology Newsletter (MJ Santos).



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Plant Systematics and Bioinformatics


1. Group Name / denomination

Plant Systematics and Bioinformatics


Cymon John Cox






- 39.754 EUR, FCT, PTDC/BIA-BCM/099565/2008 - The origin and early diversification of plants: a phylogenomic approach employing novel composition-heterogeneous methods.


1. Objectives

Our group has two major research themes: evolutionary plant systematics and bioinformatics. As phylogenetic systematists our primary research aim is the reconstruction of ancestral relationships among organisms using molecular and morphological character data. The resulting evolutionary trees inform us of past trajectories of organismal traits, aspects of paleo-biodiversity, and provide the context for phyto-geographic analyses. Evolutionary process modeling is an integral part of organismal systematics and increasing computational power has enabled us to apply evermore complex models. In this regard, we are particularly interested in both across-data and temporal forms of substitution process heterogeneity in maximum likelihood and Bayesian analytical contexts. Although often ignored by phylogenetists, process heterogeneity is present at all level of the tree of Life and its accommodation in our models is having an increasingly profound impact on phylogenetic systematics. The enormous growth in the availability of genetic data, and the increasing ease in which new data are obtainable, has meant that systematists are becoming increasingly reliant on bioinformatic frameworks to manage and manipulate the large amounts of data they employ. Moreover, the proliferation of phylogenetic software has necessitated that systematists are able to interpret the utility of new


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algorithms and software, as well as implement their own ideas in novel analyses. We are particularly interested in the development of new bioinformatic software for the manipulation and analysis of phylogenetic data.


The Postdoctoral Research Assistant Joao Sollari Lopes was hired in April to participate in the FCT funded project “The origin and early diversification of plants: a phylogenomic approach employing novel composition-heterogeneous methods” (PTDC/BIA-BCM/099565/2008, PI Cox). Despite the late hire of the PDRA the project time-line has remained on schedule.

During 2010 the PSB group has enlarged the computation cluster facility housed in the UALG Datacentre (http://gyra.ualg.pt). The cluster now consists of 5 machines and a total of 80 CPUs: 2 servers purchased from the pluriannual allowance of the group PI), 2 servers purchased from the FCT project (PTDC/BIA-BCM/099565/2008, PI Cox), and a single machine purchased by Drs. Cancela and Cruzeiro of CCMAR. The cluster also has a 8000V UPS and battery unit purchased by CCMAR. More than 60 bioinformatic software packages and analytical pipelines have been installed and the cluster mirrors the majority of data held at NCBI Genbank. In the past year, the cluster has provided a computational platform for genome assembly (Roche 454 data), phylogenetic analyses, BLAST analyses, and transcriptome assembly and expression analyses (Illumina data).

FCT funding was sought and awarded for the project “A conquista do meio terrestre: estudo filogenético da evolução do cloroplasto das Charophyta e plantas terrestres utilizando modelos de substituição heterogéneos de dados e tempo.” (PTDC/BIA-EVF/113129/2009, PI Cox).

8d. Productivity


1. Cox, CJ, Goffinet, B, Wickett, NJ, Boles, SB, Shaw, AJ. 2010. Moss diversity: a molecular phylogenetic analysis of genera. Phytotaxa 9: 175-195.

2. Shaw, AJ, Cox, CJ, Buck, WR, Devos, N, Buchanan, AM, Cave, L, Seppelt, R, Shaw, B, Larran, J, Andrus, R, Greilhuber, J, Temsch, EM. 2010. Newly resolved relationships in an early land plant lineage: Bryophyta class Sphagnopsida (peat mosses). American Journal of Botany 97: 1511-1531.

3. Shaw, AJ, Devos, N, Cox, CJ, Boles, SB, Shaw, B, Buchanan, AM, Cave, L, Seppelt, R. 2010. Peatmoss (Sphagnum) diversification associated with Miocene Northern Hemisphere climatic cooling? Molecular Phylogenetics and Evolution 55: 1139-1145.

4. Batini, C, Lopes, J, Behar, DM, Calafell, F, Jorde, LB, van der Veen, L, Quintana-Murci, L, Spedini, G, Destro-Bisol, G, Comas, D. 2010. Insights into demographic history of african pygmies from complete mitochondrial genomes. Molecular Biology and Evolution 28: 1099-1110.


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5. Conceição, N, Cox, CJ, Simões, B, Viegas, M, Cancela, ML. 2010. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. The Journal of Applied Ichthyology 26: 245-250.


1. Lopes, JS. 2010. 10 years of Software development for approximate Bayesian computation. In: Clary, TS (Ed). Horizons in Computer science Research, Nova Science Publishers, Hauppauge.







All of the group productivity is done within an international context and in collaboration with various international experts within the field. These collaborations have resulted in 5 publication is international peer-reviewed journals.

The PI was invited to speak at the SMBE meeting in Lyon, France on the topic of eukaryotic cell evolution. The PI reviewed articles for the international peer-review journals: Systematic Biology, Molecular Biology and Evolution, BMC-Evolutionary Biology, and The Bryologist. The PI also examined a MSc. Thesis submitted at the Natural History Museum, London, England.



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Synthesis and Organic Reactivity


1. Group Name / denomination

Group of Synthesis and Organic Reactivity


Maria Lurdes Santos Cristiano




- FCT Pluriannual funding.

- National funding

7.756 EUR, FCT PTDC/QUI/67674/2006, Studies on the reactivity of derivatives of tetrazole and isothiazole: isothizazolyl-tetrazoles as potential bidentate ligands and their application in catalysis.


1. Objectives

The research in the group is directed to two main areas: Physical Organic Chemistry and Medicinal Chemistry.

Physical Organic Chemistry

- Structure-reactivity relationships provide valuable information for the interpretation and prediction of the outcome of reactions. as this approach may be applied to all reacting systems. For instance, it establishes grounds for the elucidation and interpretation of the interactions between pharmacologically active compounds and their biological targets, providing essential information for drug design and optimization.

- Our research involves synthesis, structural analysis and investigation of the reactivity of derivatives of heterocyclic bioactive compounds. The aim is the improvement and fine-tuning of properties, for instance the optimization of biological activity, and also the development of new synthetic methods applicable to the preparation of bioactive compounds and to the modification of natural products. Structural elucidation and


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mechanistic investigations are approached, in catalysed and non-catalysed reactions. During 2010, we focused essentially on the investigation of the structure, photochemistry and thermochemistry of bioactive derivatives of tetrazole, benzisothiazole (saccharin) and endoperoxides.

Medicinal Chemistry

- Within this area we aim to develop hybrid endoperoxide antimalarial drugs. Two types of these chimeric drugs are targeted. In one of them, an endoperoxide is coupled with a plasmodial DNA-binding agent and in the other with a plasmodial protease inhibitor. The rationale is based on the ability of Fe(II) to selectively cleave the peroxide bridge, generating radical species (ROS or carbon-centered radicals) and liberating the partner drug (DNS-binder or protease inhibitor). Activation can occur in the cytosol or inside the digestive vacuole of plasmodia spa. If the drug target is DNA, the active species produced should be capable of simultaneous selective oxidative damage to a complementary strand and of non-covalent interaction to the helix, in a combination chemotherapy-like approach. If the endoperoxide is coupled with a plasmodial cysteine protease inhibitor, the two pharmacophores will be liberated inside the digestive vacuole. Because tumor cells are known to have higher Fe(II) concentrations than normal cells, the DNA-directed peroxide compounds designed may also act as potential antiproliferative agents in various cancer cell lines. Several artemisinin-type compounds have demonstrated high cytotoxic activity against various tumor cell lines.

- Within ARTEMIP, the aim is to investigate the embriotoxicity and teratogenicity of Artemisinin-type drugs. The work packages under the responsibility of the University of Algarve are related with the synthesis of probe peroxides with demonstrated antimalarial activity (affinity labelled, radio-labelled or fluorescent) in order to assess distribution and accumulation and elucidate the mechanism of action.


Under the research topic of reactivity, research in the area of Physical Organic Chemistry was continued. The studies were directed to the investigation of structure and reactivity of bioactive derivatives of tetrazole, benzisothiazole (saccharin) and endoperoxides. Important results were published concerning the structure and photochemistry of bioactive tetrazoles and the effect of hydrogen-bonding in the properties of nitro aromatics.

A major investment was made in the design and synthesis of endoperoxide-based antimalarials and falcipain inhibitors. The targets are hybrid drugs, designed within the concept of combination chemotherapy, whereby two active pharmacophores, directed to different intracellular targets, are combined within the same chemical entity. In vitro and in vivo tests for our compounds are conducted at the Liverpool School of Tropical Medicine and at the Department of Pharmacology of the University of Liverpool. Major results were published in Journal of Medicinal Chemistry.

A big effort was made towards meeting the objectives of the ARTEMIP project, started in March 2008. Contracted deliverables for the workpakage related to synthesis were provided. Results of biological tests on the compounds delivered have called for the synthesis of other analogues, which has been conducted. A set of radio-labelled and fluorescent probes was provided and


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tested by other partner institutions, namely The Liverpool School of Tropical Medicine, the University of Liverpool, the University of Gotemborg, the University of Leiden and WHO/TDR.

Collaboration was established with the EDGE group of CCMAR, with the aim of evaluating the activity of antiplasmodial endoperoxides on Perkinsus olseni. A range of endoperoxide compounds was prepared and tested on cultures of P. olseni, and preliminary results show that the compounds act as growth inhibitors of P. olseni in the micromolar range. Studies were conducted in order to evaluate the effect of Fe(II) concentration on activity and the rôle of the peroxide bridge on activity. Results point to a Fe(II) concentration dependent mechanism of activation/growth-inhibition. Further investigation is planned, directed to an evaluation of the mechanisms of action and accumulation. Some endoperoxides linked to a fluorophore label have been prepared, following analysis by confocal microscopy.

8d. Productivity


1. Johnstone, RAW, Loureiro, RMS, Cristiano, MLS, Labat, G. 2010. Bond Energy/Bond Order relationships for N-O linkages and a quantitative measure of ionicity: the role of nitro groups in hydrogen bonding. ARKIVOC 2010(v): 142-169.

2. Ismael, A, Cristiano, MLS, Fausto, R, Gomez-Zavaglia, A. 2010. Tautomer selective photochemistry of 1-(tetrazol-5-yl)ethanol. Journal of Physical Chemistry A 114: 13076-13085.

3. Frija, LMT, Ismael, A, Cristiano, MLS. 2010. Photochemical transformations of tetrazole derivatives: applications in organic synthesis. Molecules 15(5): 3757-3774.

4. Gibbons, P, Verissimo, E, Araujo, NC, Barton, V, Nixon, GL, Amewu, RK, Chadwick, J, Stocks, PA, Biagini, GA, Srivastava, A, Rosenthal, PJ, Gut, J, Guedes, RC, Moreira, R, Sharma, R, Berry, N, Cristiano, MLS, Shone, AE, Ward, SA, O’Neill, PM. 2010. Endoperoxide Carbonyl Falcipain 2/3 Inhibitor Hybrids; Towards Combination Chemotherapy of Malaria through a Single Chemical Entity. Journal of Medicinal Chemistry 53: 8202-8206.



1. Araújo, NCP, Bringela, A, Veríssimo, E, O’Neill, PM, Cristiano, MLS. 2010. New peptidic

cysteine protease inhibitors, Revista Portuguesa de Farmácia, vol. LII (4): 32.

2. Araújo, NCP, Cabral, LIL, Pensée, L, Sabbani, O’Neill, PM, Cristiano, MLS. 2010. Design and

synthesis od endoperoxide hybrids directed to plasmodial DNA, Revista Portuguesa de Farmácia,

vol. LII (4): 116.


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MASTER THESES

Guerreiro, Bruno Emanuel 2010. Endoperóxidos com actividade antimalárica: estrutura, reactividade e actividade. Master thesis in Pharmaceutical Sciences, Faculty of Science and Technology, University of Algarve. Supervisor: Maria de Lurdes Cristiano.

Czepiel, Aneta 2010. Synthesis of saccharyl tetrazoles and investigation of their potential as nitrogen ligands. Master thesis in Chemistry, Faculty of Science and Technology, University of Algarve. Supervisor: Maria de Lurdes Cristiano.



20th International Conference on Physical Organic Chemistry, 22-28 August 2010, Busan, South Korea. (Maria de Lurdes Cristiano - Member of the International Advisory Board).


8. Internationalization (Collaborative publication, Research, Graduate Training Networks or other forms of participation of the Research Group at the international level)

The Synthesis and Organic Reactivity group maintains international collaborations with the groups of:

- Prof. Paul O’Neill, Department of Chemistry, University of Liverpool;

- Prof. Kevin Park, Department of Pharmacology, University of Liverpool;

- Prof. Stephan Ward, Liverpool School of Tropical Medicine;

- Prof. Piero Olliaro, WHO/TDR;

- Prof. Bob Van der Water, Toxicology and Drug Safety Sciences, University of Leiden;

- Prof. Michael Ashton, Institute of Neuroscience and Physiology, Department of Pharmacology, University of Gothenburg;

- Dr. Andrea Gomez-Zavaglia, Centro de Investigación y Desarrollo en Criotecnología de Alimentos (Conicet La Plata, UNLP), ARGENTINA;

- Prof. Robert Johnstone, Department of Chemistry, University of Liverpool.


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9. Government/organization contract research (Work carried out by the Research Group that resulted in a publication or report. Of particular importance are those involved in public policy advice)

The PI of the group belongs to the following organizations: - IUPAC, Subcomitee on Structural and Mechanistic Chemistry; Portuguese

representative; - International standing committee for European Symposia on Organic Reactivity; - Technical Group to support FP7 – Health theme.


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9. OTHER LA ACTIVITIES

9a. Services and resources

1. Internal services and resources (Sharing of equipment and other resources both within the LA, between LAs or with other institutions of Higher Education nationally or internationally)

CIMAR LA occupies infrastructures in two distinct locations, one at the University of Porto (ca 3500 m2) and another one at the University of Algarve (ca 1800 m2). Each infrastructure includes facilities (1000 m2) to maintain aquatic organisms with an overall capacity of 60000 liters of seawater and freshwater. CIMAR researchers have also access to an aquaculture experimental station located in the Algarve (ca 2 ha). CIMAR acquired two small vessels (12 and 17 meters), one was completely operational for coastal and estuarine campaigns in 2009 and another is being equipped.

In 2009, an within the frame of the University of Porto Park of Science and Technology, the proposal for the new building that will hold CIIMAR was submitted and accepted for funding (ERDF).

Several medium and large size equipment was acquired in the last few years through a competitive national programme for scientific re-equipment and FCT funding (2 million EUR), mostly for analytical chemistry and molecular and genetic research (a list of the equipment is presented in the 2008 report). Through another funding program, an Isotope Ratio Mass Spectrometers (IRMS) was also acquired. The equipment is available to other LAs and Higher Education Institutions. The reverse is also true for access to equipment in other institutions Smaller pieces of equipment have been continuously acquired via diverse projects (from FCT and other sources).

CIMAR High Performance Computer (HPC - 160 CPUs, 360 GB of RAM, 4 TB of storage space, http://nautilus.ciimar.up.pt), used for parallel computing (e.g. to run ocean circulation numerical models, meteorological forecasting models, etc.), was fully operational in 2009. The North Iberian Ocean Observatory (RAIA) allows access to updated databases of satellite remote sensing data and numerical model solutions for the NE Atlantic. CIMAR HPC is an integral part of the University of Porto computer network and there are plans to be an active stakeholder of the IBERGRID as well as EGEE (http://www.eu-egee.org/).

CIMAR is partner in several initiatives, such as the European Network of Marine Institutes and Stations - MARS (www.marsnetwork.org), the European Marine Biological Research Centres - EMBRC (www.embrc.eu) and the ASSEMBLE FP7 infrastructure project (www.assemblemarine.org), that facilitate collaborations and shared access to infrastructures and to a comprehensive set of coastal marine ecosystems and marine organisms, research vessels and state-of-the-art experimental genomic and proteomic facilities.

Other resources shared with national and international institutions include: cell culture facilities and cell lines (e.g. Univ Bergen, NIFES, Univ Ghent), zebrafish culture facilities (IMM-Institute for Molecular Medicine, Lisbon).


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In the framework of the agreement between FCT and the Portuguese Hydrographic Institute, CIMAR researchers have also the possibility to hire ship time at an affordable cost.

Internal (and external) services for analytical chemistry and molecular and genetic research are provided. Administration and technical support is composed of 24 staff, including public and international relations, science and technology management, project management, finance, IT systems, image and communication, law, biotery, and maintenance.

Through several protocol agreements with higher education institutions, CIMAR has been supporting pre and post graduation training by providing facilities and resources including the participation of non-academic staff in teaching activities.

2. External services and resources (Scientific or technical services provided both nationally or internationally to Governments/Institutions or the community)

CIMAR has been increasingly committed in the support of public policies. Most relevant activities to provide scientific and technical services both nationally and internationally to Governments/Institutions and the community are:

- Member of European Science Foundation – Marine Board.

- Founding member of the R&D Consortium OCEANOS, submitted and approved for funding by FCT in 2009, that aim to reinforce the State Laboratories and provide scientific basis for advice and policy based on research of excellence on marine science and technology at an internationally competitive level; join action of 5 State Laboratories, 1 Associated Laboratory (CIMAR), 4 Universities and 1 R&D network (http://alfa.fct.mctes.pt/apoios/unidades/consorcios/).

- Founding member of the Institute for the Development of the Knowledge and the Economy of the Sea (IDCEM - Agency for the promotion of scientific knowledge, valorization of research and technology service agreements, and promotion of entrepreneurship).

- Member of the Maritime Cluster - OCEANO XXI, through IDCEM, a cluster of enterprises and research centres that aim to promote and strengthen the maritime economy.

- Contribution to the implementation of the Ocean Agenda of the North Region, North Regional Coordination and Development Commission (CCDR-N) (http://www.ccr-norte.pt/pagina.php?p=91); Leadership of the Ocean agenda for the territorial cooperation North of Portugal - Galicia.

- Contribution to the Regional Spatial Planning of Algarve, CCDR-Algarve.

- Contribution to the implementation of the Water Framework Directive, National Institute for Water (INAG) (eg. Project EEMA - Ecological status of coastal and transitional waters of the Portuguese coast).

Moreover, CIMAR has been involved in several R&D projects aimed to give support to European Territorial cooperation (eg. Interreg Atlantic Area, Interreg Sudoe, Interreg POCTEP) and has

http://alfa.fct.mctes.pt/apoios/unidades/consorcios/


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been regularly requested by the government to give advice on legislation (eg. Environment and fishing) and on bilateral agreements involving Portugal.

At the individual level, members of the Direction of CIMAR have also been involved in several activities:

- National delegate for FP7 (“Environment, including Climate Change” Theme) (J Coimbra).

- Director of IDCEM and Vice-President of OCEANO XXI (http://www.oceano21.org) (J Coimbra).

- Focal Point of the Minister of Science and Technology on the National Ocean Strategy (Task Group for Maritime Affairs - EMAM) (J Coimbra).

- Members of the Councils of Hydrographic Regions, assisting and advising the Administrations of the North and Algarve Hydrographic Regions (ARH-Norte, AHR-Algarve).

- Two members of CIMAR were elected as President of the Society of Environmental Toxicology and Chemistry - SETAC Europe (L Guilhermino) and Vice-President of the Iberian Association of Comparative Endocrinology (D Power).

- A member acted as regional focal point of FCT for the ERA-NET BIOME (C Delgado). Moreover, CIMAR members participate regularly in national (FCT) and international evaluation panels, and are Associate or Advisory Editors of several international publications.

CIMAR provided services for the industry in several areas including water quality (chemical, biological and toxicological), diet formulation for aquaculture, DNA extraction and genotyping, physical oceanographic data collection and environmental consulting studies.

Both CIIMAR and CCMAR have been qualified for providing R&D and innovation services to SMEs under the Portuguese National Strategic Reference Framework (QREN, 2007-2013).

9b. Networking actions

1. Networking actions (Both national and international activities)

Most relevant:

MEMBER OF EUROPEAN SCIENCE FOUNDATION – MARINE BOARD (www.esf.org/research-areas/marine-board.html)

- FP7 COORDINATION ACTIONS AND NETWORKS OF EXCELLENCE


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- KNEU- Developing a Knowledge Network for European Expertise on biodiversity and ecosystem services to inform policy making and economic sectors

- EUROMARINE - Integration of European Marine Research Networks of ExcellencE

- MarBEF + - Marine Biodiversity and Ecosystem Functioning

MARINE RESEARCH CENTRES NETWORKS

- MARS - European Network of Marine Institutes and Stations (www.marsnetwork.org)

- ASSEMBLE - Association of European Marine Biological Laboratories (EU FP7, www.assemblemarine.org)

- EMBRC - European Marine Biological Research Centres, ESFRI infrastructure (www.embrc.eu)

TECHNOLOGICAL PLATFORMS

- European Aquaculture Technology and Innovation Platform (www.eatip.eu)

- Water Supply and Sanitation Technology Platform (www.wsstp.eu)

ESF NETWORKING PROGRAMMES

- Functional Dynamics in Complex Chemical and Biological Systems (http://funcdyn.org)

COST ACTIONS (www.cost.esf.org)

- Development and implementation of a pan-European Marine Biodiversity Observatory System – EMBOS, ES1003

- Improved constrains on models of glacial isostatic adjustment, ES0701

- Welfare of fish in European aquaculture - n. 867

- Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network - n. FA0801

- Providing the scientific basis for managing the goods and services arising from European seagrassecosystems under anthropogenic pressure – n. ES0906

- Integrating ice core, marine and terrestrial records (60.000 to 8.000 years ago) – n. ES0907

INTERNATIONAL THEMATIC NETWORKS

- EPBRS (www.epbrs.org), and the supportive network Biostrat

- Ocean Tracking Network (http://oceantrackingnetwork.org)

http://www.marsnetwork.org/

http://www.eatip.eu/

http://oceantrackingnetwork.org/


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- Seagrass Net Monitoring Program (www.seagrassnet.org)

- ICES Working Groups: Habitat Mapping; Biological Effects of Environmental Contaminants (www.ices.dk)

- Red Iberoamericana de Toxicología y Seguridad Química (http://ritsq.org)

- European Atlantic Periphery Marine Socio-Economic Network

NATIONAL THEMATIC NETWORKS

- R&D National Consortium OCEANOS

- DivMar - National network on Costal Marine Biodiversity (www.cimar.org/divmar)

EDUCATION AND TRAINING NETWORKS

- European Network in Aquaculture, Fisheries & Aquatic Resources Management (www.aquatnet.com)

9c. Training

1. Training activities (Activities of the LA as a whole)

In 2010, 70 MSc students and 22 PhD students completed their degree under (co)-supervision of CIMAR members.

PRE-GRADUATION STUDIES

- Undergraduated students incorporated in R&D project teams, with Integration into Research Grants (BII), and in the pluridisciplinary program of Univ Porto on Microbial Life in Extreme Antarctica Environments.

EUROPEAN AND NATIONAL PHD AND MSC PROGRAMMES

- Doctoral School in Marine and Environmental Sciences

- Erasmus Mundus MARES – Doctoral Programme on Marine Ecosystem Health and Conservation (www.mares-eu.org)

- Erasmus Mundus Master in Science in Marine Biodiversity and Conservation (www.embc.marbef.org)

- European Master in Aquaculture and Fisheries

- Master in Ecological Management of Catchments in Europe

- Master in Marine Sciences - Marine Resources, Univ Porto

http://www.seagrassnet.org/

http://www.ices.dk/

http://ritsq.org/

http://www.mares-eu.org/

http://www.embc.marbef.org/


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- Master in Environmental Contamination and Toxicology, Univ Porto

- Master in Aquaculture and Fisheries, Univ Algarve

- Master in Marine Biology, Univ Algarve

- Master in Biomedical Sciences, Univ Algarve

OTHERS

- ASEM-duo Fellowship Programme with Burapha Univ, Thailand

- Advanced Courses on Marine Ecotoxicology, 26-30 April; Freshwater Ecotoxicology, 1-5 March; Biomarkers, 22-26 March; Porto

- 5th Radiation Safety Course, 31 May - 1 June, Faro

- Scientific Illustration Courses, 22-28 May, Faro; 8-6 June, Porto

- LARVITA Fish Larvae Training School, 22-26 Nov, Faro

- Workshop on “Genetic Alterations in Aquatic Organisms after Exposure to Pollutants”, 12-13 Jan, Porto

- Workshop on "Sustainable Extensive and Semi-intensive Aquaculture Production in Southern Europe”, 20-21 January, Tavira

- 4th SPB Clinical Biochemistry Workshop, 29 Jan, Faro

- Workshop on “New Tools for better planning, response management and damage assessment in HNS, inert and oil spills”, 30 April, Lisbon

- I Workshop on “Marine Biodiversity in Arrabida”, 22 May, Sesimbra

- Workshop on “Computer Simulations of Nanobiological Systems”, 27 May, Faro

- Workshop on “Sea-level rise along the Portuguese coast”, 28 May, Porto

- 2nd UTEN Workshop 2010 – Marine and Bio-Sciences, Research Collaboration & Network Building for Commercialization, 27-28 Sept, Faro

- I European Seagrass Workshop, 30 Sept - 1 Oct, Portinho da Arrábida

- 4th Workshop on “Coastal Altimetry”, 14-15 Oct, Porto

- Workshop on “Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network”, 5-6 Oct, Porto

INTERNAL SEMINARS IN FARO AND PORTO ON A FORTNIGHT BASIS


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9d. Outreach/science and society

1. Outreach/science and society (Activities that the LA as a whole)

CIMAR has performed a significant effort in the promotion of outreach/Science and Society activities, to foster the dialogue between scientists and society, to improve the public knowledge and perception of science and to increase the interest of the young students towards scientific careers.

SCIENTIFIC MANAGEMENT OF PUBLIC EQUIPMENT

CMIAs – Environmental Monitoring and Interpretation Centres:

- CIMAR manages two centres for environmental interpretation and monitoring through agreements with the municipalities: CMIA-Vila do Conde (www.cmia-viladoconde.net) and CMIA-Matosinhos (www.cmia-matosinhos.net). During 2010, CMIAs displayed 7 temporary exhibitions, organized conferences and performed regular in house and field activities for public of different ages. A Scientific Culture fortnight (11-29 Nov), an intensive program of science outreach for the general public that included 6 conferences, 3 exhibitions, 4 workshops and 1 field trip was also organized.

Public aquariums and fisheries museums:

- CIMAR researchers manage two public aquariums and fisheries museums: Aguda Littoral Station - ELA (www.fundacao-ela.pt, ca 50000 visitors/year) and River Minho AQUAMuseum (www.cimsoft.pt/aquamuseu, ca 30000 visitors/year). Both stations have a special relevance in what concerns Environmental Education Programs.

PARTICIPATION IN THE CIÊNCIA VIVA PROGRAMME (http://www.cienciaviva.pt)

Several types of activities have been implemented at both CCMAR and CIIMAR, to contribute to the promotion of a scientific and technological culture among the Portuguese population and more specifically to encourage the use of experimental methods in science learning, involving both scientific and school communities.

- Scientists go to School: presentation of scientific lectures at elementary and high schools (23 lectures throughout Algarve).

- Science on Holidays for Young People aiming to offer high school students the opportunity for first-hand contact with the reality of scientific research work during their summer vacations: scientific internships for 24 students (5-10 days).

- Biology Activities in the Summer: 38 activities (4-8 hours each) for general public of all ages, in particular family groups.

- “Café de Ciência - Science for Biodiversity”, Assembly of the Republic, 17 March.

- Science and Technology Week, 22-28 Nov.


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- “First encounters with Science”, in cooperation with CCViva of Tavira, 23-27 Nov.

SEA ITINERANT UNIVERSITY (UIM) (http://www.cimar.org/CIIMAR/en/hz_pas_uim.htm)

- UIM is a program that aims to promote the maritime identity of Portuguese and Spanish university students, through the contact with the sea and its potentialities, related activities and culture. Courses are organised in land and on board the vessel CREOULA, a 4-masted training ship of the Portuguese Navy built in 1937. The theme of the 2010 course was "Knowledge and adventure".

OTHER EVENTS FOR HIGH SCHOOL STUDENTS:

- CIMAR regularly participates in events and exhibitions aimed at encouraging high schools students to engage in scientific careers:

- “Junior University” (http://universidadejunior.up.pt/), within the School of Life and Health Sciences.

- “Science, Education and Innovation” exhibition by the University of Porto (25-28 March).

- LAB-IT®-The Itinerant Laboratory in Molecular Genetics.

- Collaboration with the Visionarium - Europarque Science Centre to implement a national project using Daphnia as biological model for experimental teaching of sciences in high schools (http://visionarium-daphnia2.blogspot.com/).

- MOBIDIC - School in the Beach, an outreach program for intertidal biodiversity monitoring in beaches of the North coast (http://www.estgm.ipb.pt/~projig/mobidic).

- Both CIIMAR and CCMAR received visits from schools throughout the year.

OTHER EVENTS FOR THE GENERAL PUBLIC

- European initiative “Reseachers’ Night”, 24 Sept (http://www.cientistasaopalco.com/).

- “Bioblitz – Looking for marine biodiversity”, 4-5 Sept (http://www.bioblitzportugal.com/).

- Birdwatching – Discover Birds and Birdlife in the Algarve

- EXPOMAR exhibition, 28 April-2 May (http://expomar.efeitoeventos.com/).

OTHER DISSEMINATION ACTIVITIES

Other activities included the development of a new website for CIIMAR (www.ciimar.up.pt), the release of CIIMAR Newsletter - WAVES, pages in social networks (e.g. Facebook, Twitter, Youtube, Google maps, Vimeo) and the organization of weekly internal seminars at both centres.

http://visionarium-daphnia2.blogspot.com/

http://www.cientistasaopalco.com/

http://www.bioblitzportugal.com/


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CIMAR IN THE MEDIA

Dissemination was also channeled through the media: publications in non-scientific journals and newspapers (e.g. Alphagalileo, Ciência Hoje, JN, DN, Expresso, Agência Lusa, Visão, National Geographic), participation in TV and radio programs (e.g. SIC, Rádio Renascença, Antena 1).

9e. International events

1. Organization of international events (Events that are international in dimension and required the involvement of the LA for their implementation)

INTERNATIONAL CONGRESSES AND SYMPOSIUMS

- Aquaculture Europe 2010 - Seafarming Tomorrow, European Aquaculture Society, 5-8 October, Porto, Portugal (ca 1100 attendees, 55 countries)

- FloraMac2010, 23-25 Sept, Ponta Delgada, Portugal

- Iberian Symposium on River Minho Hydrographic Basin, 19-20 Nov, Vila Nova de Cerveira, Portugal.

INTERNATIONAL WORKSHOPS

- Workshop PETGENES - Genetic Alterations in Aquatic Organisms after Exposure to Pollutants, 12-13 January, Porto, Portugal

- Workshop on "Sustainable Extensive and Semi-intensive Aquaculture Production in Southern Europe”, 20-21 January, Tavira, Portugal (130 attendees)

- 4th SPB Clinical Biochemistry Workshop, Interaction between biochemistry and clinical practice, 29 January, Faro, Portugal

- Workshop on “New Tools for better planning, response management and damage assessment in HNS, inert and oil spills”, 30 April, European Agency for Maritime Safety, Lisbon, Portugal

- I Workshop on “Marine Biodiversity in Arrabida”, 22 May, Sesimbra, Portugal

- Workshop on “Computer Simulations of Nanobiological Systems”, 27 May, Faro, Portugal

- Workshop on “Sea-level rise along the Portuguese coast”, 28 May, Porto, Portugal

- 2nd UTEN Workshop 2010 – Marine and Bio-Sciences, Research Collaboration & Network Building for Commercialization, 27-28 Sept, Faro, Portugal http://utenportugal.org/events/marine-science-workshop/

- I European Seagrass Workshop, 30 Sept - 1 Oct, Portinho da Arrábida, Portugal

http://utenportugal.org/events/marine-science-workshop/


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- 4th Workshop on “Coastal Altimetry”, 14-15 Oct, Porto, Portugal

- Workshop on “Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network - LARVANET”, 5-6 Oct, Porto, Portugal.

AS MEMBERS OF SCIENTIFIC COMMITTEES (e.g.)

- SETAC Europe 20th Annual Meeting, 23- 27 May, Spain

- Congress on Deep Water Processes and Products, 16-18 June, Spain

- 9th International Congress on the Biology of Fish, American Fisheries Society, 5-8 July, Spain

- XV Congress of the Iberian Association of Limnology, 5- 9 July, Portugal

- 8th International Conference on Toxic Cyanobacteria, 29 August - 4 Sept, Turkey

- XVI Iberian Symposium on Marine Biology, 6-10 Sept, Spain

- XIV National Congress of Parasitology, 8-10 Sept, Portuga

- National Congress from the Portuguese Society of Biochemistry, 15-17 Dec, Portugal

- 20th International Conference on Physical Organic Chemistry, 22-28 August, South Korea

INTERNAL SEMINARS AT CIIMAR AND CCMAR, weekly/monthly along the year


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10. INTERNAL EVALUATIONS

10.1 Summary of internal evaluations during 2010

During 2010, the contact with members of the External Advisory Board was maintained, mainly with Prof. Steve Hawkins and mainly for follow up of previous recommendations for promoting the research capability in the general area of marine biodiversity and ecosystems science. The recommendations have been implemented. Meanwhile, we had foreseen an internal evaluation exercise by the External Advisory Board, to be held simultaneously with the annual meeting of CIMAR-LA, as we were expecting the results of the external evaluation carried out by FCT in 2008 during the 2nd semester of 2009, but only delivered to us in 2010. In sequence of this external examination, which was very positive and resulting only in minor suggestions, the evaluation panel concluded that the CIMAR continued to merit its LA status. Facing the conclusion of this external evaluation, the inexistence of major criticisms or recommendations, and the eminence/start of discussions with Ministry of Science and Technology and FCT as to an eventual new contract for the LA, we concluded that a new internal exercise by the External Advisory Board would really make sense only after the definition of the future status of the LA.

10.2 Future internal evaluations programmed for 2011

In the light of recent announcement of a new governmental contract with the CIMAR LA, which can potentially cover the next decade, pending on the evaluation results at mid-term, we envisage the following strategic actions as to the future internal evaluations: 1) to rethink and likely remodel the External Advisory Board, to better incorporate external feedback on our activities in physical oceanography and coastal dynamics, areas that were reinforced with new researchers in the past years; 2) to schedule a first working visit with the renewed External Advisory Board, soon after signing the new contract and focusing on the new starting-point of the LA and on the 5-year strategic program that is associated with the emergence of the new LA contract; 3) to schedule an annual visit of the External Advisory Board, coinciding with the annual meeting of the LA; 4) to capitalize on the use of the available video-conference tools to promote more regular discussions with each and every expert of the External Advisory Board.


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SCIENTIFIC OUTPUT 2010

A. PUBLICATIONS

EDITED BOOKS

1. Almeida, JR, Sá, N. 2010. Guia de Mergulho da Madeira (Madeira Diving Guide). Veraçor Editores, Ponta Delgada, Portugal, 109 p.

2. Duarte, P, Santana-Casiano, JM. 2010. Oceans and the Atmospheric Carbon Content. Springer, 176 p. ISBN: 978-90-481-9820-7.

3. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2010. Diversidade dos Parasitas de Peixes de Água Doce do Brasil. Clichetec, Maringá, Brasil, 333 p.

4. Fidalgo, ML, Sousa, L, Oliveira, A, Magalhães, P. 2010. Norte do Sonho. Caminha e o Estuário do Rio Minho. Edição da Câmara Municipal de Caminha, Caminha, Portugal, 165 p. ISBN: 978-989-95920-4-9.

5. González-Díaz, H, Agüero-Chapin, G, Munteanu, CR, Prado-Prado, F, Chou, KC, Duardo-Sanchez, A, Patlewicz, G, López-Díaz, A. 2010. Alignment-free Models in Plant Genomics: Theoretical, Experimental, and Legal Issues - Agriculture Issues and Policies. Nova Science Publishers Inc., NY, USA, 78 p. ISBN: 978-1-60692-638-3.

BOOKS (Chapters)

1. Afonso, MJ, Pires, A, Chaminé, HI, Marques, JM, Guimarães, L, Guilhermino, L, Rocha, FT. 2010. Aquifer vulnerability assessment of urban areas using a GIS-based cartography: Paranhos groundwater pilot site, Porto, NW Portugal. In: Paliwal, BS (Ed). Global Groundwater Resources and Management. Scientific Publishers, Jodhpur, pp. 259-278. ISBN: 978-8172336196.

2. Agüero-Chapin, G, Sánchez-Rodríguez, A, Antunes, A, de la Riva GA, González Díaz, H. 2010. Network entropies classification of fungi and bacteria of fungi and bacteria cellulases of interest for biotechnology. In: González-Díaz, H, Munteanu, CR (Eds). Topological Indices for Medicinal Chemistry, Biology, Parasitology, Neurological and Social Networks. Transworld Research Network, Kerala, India, pp. 69-95. ISBN 978-81-7895-989-9.

3. Araújo, R, Cabral, JP. 2010. Fungal air quality in medical protected environments. In: Kumar, A (Ed). Air Quality. InTech. ISBN 978-953-307-131-2.

4. Bastos, L, Bos, MS, Fernandes, RMS. 2010. Deformation and tectonics: Contribution of GPS measurements to plate tectonics – Overview and recent developments. In: Xu, Guochang (Ed). Sciences of Geodesy - I, Advances and Future Directions, pp. 155-184. ISBN 978-3-642-11740.

5. Bio, A, Meirinho, A, Ramírez, I, Geraldes, P. 2010. Empirical modelling of Balearic shearwater (Puffinus mauretanicus) census data for the Portuguese Atlantic. In: Calado, H, Gil, A (Eds). Geographic Technologies Applied to Marine Spatial Planning and Integrated Coastal Zone


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Management. Centro de Informação Geográfica e Planeamento Territorial, Azores University, pp. 23-30. ISBN 978-972-8612-64-1.

6. Brown, CL, Power, DM, Núñez, JM. 2010. Disorders of development in fish. In: Leatherland, JF, Woo, PTF (Eds). Fish Diseases and Disorders, Vol 2, 2nd

edition, CABI, Oxon, UK, pp. 166-181.

ISBN 978-1-84593-553-5.

7. Cancela, ML, Bargelloni, L, Boudry, P, Boulo, V, Dias, J, Huvet, A, Laizé, V, Lapègue, S, Leite, R, Mira, S, Nielsen, EE, Planas, JV, Roher, N, Sarropoulou, E, Volckaert, FAM. 2010. Genomic Approaches in Aquaculture and Fisheries. In: Cock, JM, Viard, F, Tessamar-Raible, K, Boyen, C (Eds). Introduction to Marine Genomics, Springer-Verlag, Heidelberg, pp. 213-286. ISBN 978-90-481-8616-7.

8. Carvalho, AR, Barros, N, Duarte, P. 2010. Impact of Oporto metropolitan area carbon dioxide emissions over the adjacent coastal zone. In: Duarte, P, Santana-Casiano, JM (Eds). Oceans and the Atmospheric Carbon Content, Springer, pp. 23-46. ISBN 978-90-481-9820-7.

9. Conceição, LEC, Aragão, C, Rønnestad, I. 2010. Protein metabolism and amino acid requirements in fish larvae. In: Cruz-Suarez, LE, Ricque-Marie, D, Tapia-Salazar, M, Nieto-López, MG, Villareal-Cavazos, DA, Gamboa-Delgado, J (Eds). Avances en Nutrición Acuícola X - Memorias del X Simposio Internacional de Nutrición Acuícola, Universidad Autónoma de Nuevo León, Monterrey, México, pp. 251-264.

10. Cruzeiro, L. 2010. Protein Folding. In: Springborg, M. (Ed). Specialist Periodic Reports of the Royal Society of Chemistry, UK, Chem. Modell., vol. 7, pp. 89-114. ISBN 978-1-84755-075-0.

11. Elmqvist, TE, Maltby, E, Barker, Las, Mortimer, CAs, Aronson, J, De Groot, R, Fitter, A, Mace, G, Norberg, J, Perrings, C, Sousa Pinto, I, Ring, I. 2010. Biodiversity, ecosystems and ecosystem services. In: Pushpam, K. (Ed). The Economics of Ecosystems and Biodiversity: Ecological and Economic Foundations. Earthscan, London, UK, pp. 41-111. ISBN 978-18-497-1212-5.

12. Granja, HM. 2010. Le combat de Roland Paskoff contre la defense lourde des côtes. In: Duvat, V, Battiau-Queney, Y, Clus-Auby, C, Prat, M-C (Eds). Roland Paskoff et les Littoraux: Regards de Chercheurs. L’ Harmattan, Paris, France, pp. 277-287. ISBN 978-2-296-13264-1.

13. Granja, HM. 2010. Les dunes côtières du nord-ouest du Portugal. In: Duvat, V, Battiau-Queney, Y, Clus-Auby, C, Prat, M-C (Eds). Roland Paskoff et les Littoraux: Regards de Chercheurs. L’ Harmattan, Paris, France, pp. 230-238. ISBN 978-2-296-13264-1.

14. Jenkins, SR, Martins, GM. 2010. Succession on hard substrata. In: Dürr, S, Thomason, JC (Eds). Biofouling. Wiley-Blackwell, pp. 60-72. ISBN: 978-14-4431-546-2.

15. Larsen, K, Froufe, E. 2010. Identification of polymorphic species within groups of morphologically conservative taxa: combining morphological and molecular techniques. In: Nimis, PL, Vignes Lebbe, R (Eds). Tools for Identifying Biodiversity: Progress and Problems. Edizione Universitá di Trieste, pp. 301-305. ISBN 978-88-8303-295-0.

16. Larsen, K. 2010. Order Tanaidacea: Tanaids. In: Gordon, DP (Ed). Species 2000 - The New Zealand Inventory of Biodiversity. Chapter 8 - Subphylum Crustacea, Volume 2, Canterbury, University Press, Christchurch, pp. 164-166.


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17. Lopes, JS. 2010. 10 years of software development for approximate Bayesian computation. In: Clary, TS (Ed). Horizons in Computer Science Research, Nova Science Publishers, Hauppauge, USA.

18. Moraes, J, Iwashita, M, Ozório, R, Rema, P, Moraes, F. 2010. Influence of vitamin E supplementation on dermal wound healing in tilapia, Oreochromis niloticus. In: De Carlo, F, Bassano, A (Eds). Freswater Ecosystems and Aquaculture Research. Nova Science Publishers Inc., New York, pp. 177-192. ISBN 978-1-60741-707-1.

19. Pais-Barbosa, J, Veloso-Gomes, F, Taveira Pinto, F. 2010. GIS tool for coastal morphodynamics analysis. In: Green, DR (Ed). Coastal and Marine Geospatial Technologies - Coastal Systems and Continental Margins, Springer, Volume 13, pp. 275-283. ISBN: 978-1-4020-9720-1.

20. Pereira, R, Yarish, C. 2010. The role of Porphyra in sustainable culture systems: Physiology and applications. In: Israel, A, Einav, R, Seckbach, J (Eds). Role of Seaweeds in Globally Changing Environments - Cellular Origin, Life in Extreme Habitats and Astrobiology. Springer Publishers, Volume 15, pp. 339-353. ISBN: 978-90-481-8569-6.

21. Power, DM, Guerreiro, PMG, Hubbard, PC, Huertas, M, Morgado, IM, Fuentes, J. 2010. Endocrinology applied to aquaculture finfish. In: Koumondouros, G (Ed). Recent Advances in Aquaculture Research, Transworld Research Network, Kerala, pp. 1-54.

22. Robinson, IS, da Silva, J. 2010. Internal waves. In: Robinson, IS (Ed). Discovering the Ocean from Space, The Unique Applications of Satellite Oceanography. Springer Verlag Berlin Heidelberg, Chapter 12, pp. 453-483. ISBN: 978-3-540-24430-1.

23. Tavares, PC, Shapouri, M, Martins, C, Pereira, P, Falcão, M, Machado, M, Cancela da Fonseca, L. 2010. Variability in sources of organic matter influencing invertebrate community as revealed by pigment levels in mesoscale experiments. In: Martins, J, Tavares, A, Tavares, M, Ribeiro, L (Eds). Paula Tavares - Uma Ciência de Rosto Humano, CVRM - Instituto Superior Técnico, Lisboa, pp. 40-63.

24. Urbatzka, R, Rocha, MJ, Rocha, E. 2010. Regulation of ovarian development and function in teleosts. In: Norris, DO, Lopez, KH (Eds). Hormones and Reproduction in Vertebrates. Academic Press, Volume 1 – Fishes, pp. 65-82. ISBN: 978-0-12-375009-9.

25. Wilson, JM, Castro, LFC. 2010. Morphological diversity of the gastrointestinal tract in fishes. In: Grosell, M, Farrell, AP, Brauner, CJ (Eds). The Multifunctional Gut of Fish. Elsevier Inc., Volume 30, pp. 1-55. ISBN: 978-0-12-374982-6.

PUBLICATIONS IN INTERNATIONAL PEER REVIEWED JOURNALS

1. Afonso, CML, Bonomolo, G, Monteiro, P, Bentes, L, Oliveira, F, Veiga, P, Rangel, MO, Sousa, I, Leite, L, Gonçalves, JMS. 2010. First record of Ocinebrina nicolai (Mollusca: Gastropoda: Muricidae: Ocenebrinae) in north-eastern Atlantic waters. Marine Biodiversity Records 3: 1-4.

2. Afonso, MJ, Chaminé, H, Marques, JM, Carreira, PM, Guimarães, L, Guilhermino, L, Gomes, A, Fonseca, PE, Pires, A, Rocha, F. 2010. Environmental issues in urban groundwater systems: a multidisciplinary study of the Paranhos and Salgueiros spring waters, Porto (NW Portugal). Environmental Earth Sciences 61: 379-392. IF=0.678.


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3. Agra, A, Guilhermino, L, Soares, A, Barata, C. 2010. Genetic costs of tolerance to metals in Daphnia longispina populations historically exposed to a copper mine drainage. Environmental Toxicology and Chemistry 29: 939-946. IF=3.026.

4. Alberto, F, Raimondi, PT, Reed, DC, Coelho, NC, Leblois, R, Whitmer, A, Serrão, EA. 2010. Habitat continuity and geographic distance predict population genetic differentiation in giant kelp. Ecology 91: 49-56. IF=5.073.

5. Alexandre, A, Silva, J, Santos, R. 2010. Inorganic nitrogen uptake and related enzymatic activity in the seagrass Zostera noltii. Marine Ecology 31(4): 539-545. IF=1.272.

6. Almeida, AM, Campos, A, Francisco, R, van Harten, S, Cardoso, LA, Coelho, AV. 2010. Proteomic investigation of the effects of weight loss in the gastrocnemius muscle of wild and New Zealand white rabbits via 2D-electrophoresis and MALDI-TOF MS. Animal Genetics 41: 260-272. IF=2.203.

7. Almeida, AM, van Harten, S, Campos, A, Coelho, AV, Cardoso, LA. 2010. The effect of weight loss on protein profiles of gastrocnemius muscle in rabbits: a study using 1D electrophoresis and peptide mass fingerprinting. Journal of Animal Physiology and Animal Nutrition 94(2): 174-185. IF=1.106.

8. Almeida, AP, Dethoup, T, Singburaudom, N, Lima, R, Vasconcelos, MH, Madalena, M, Kijjoa, A. 2010. The in vitro anticancer activity of the crude extract of the sponge-associated fungus Eurotium cristanum and its secondary metabolites. Journal of Natural Pharmaceuticals 1: 25-29.

9. Almeida, JR, Oliveira, C, Gravato, C, Guilhermino, L. 2010. Linking behavioural alterations with biomarkers responses in the European seabass Dicentrarchus labrax L. exposed to the organophosphate pesticide fenitrothion. Ecotoxicology 19: 1369-1381. IF=3.051.

10. Alves, CQ, Kijjoa, A, David, JM, David, JP. 2010. Flavonoids and benzoic acid derivatives from Diodea virgata. Planta Medica 76(12): 1284-1284. IF=2.369.

11. Alves Martins, D, Estévez, A, Stickland, N, Simbi, B, Yúfera, M. 2010. Dietary lecithin source affects growth potential and gene expression in Sparus aurata larvae. Lipids 45: 1011-1023. IF=2.151.

12. Alves, RN, Cordeiro, O, Silva, TS, Richard, N, de Vareilles, M, Marino, G, Di Marco, P, Rodrigues, PM, Conceição, LEC. 2010. Metabolic molecular indicators of chronic stress in gilthead seabream (Sparus aurata) using comparative proteomics. Aquaculture 299: 57-66. IF=2.044.

13. Antunes, F, Hinzmann, M, Lopes-Lima, M, Machado, J, da Costa, PM. 2010. Association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of Anodonta cygnea (Linnaeus, 1758). Microbial Ecology 60: 304-309. IF=2.875.

14. Aragão, C, Costas, B, Vargas-Chacoff, L, Ruiz-Jarabo, I, Dinis, MT, Mancera, JM, Conceicão, LEC. 2010. Changes in plasma amino acid levels in a euryhaline fish exposed to different environmental salinities. Amino Acids 38: 311-317. IF=4.106.

15. Araújo, C, Torres, C, Silva, N, Carneiro, C, Gonçalves, A, Radhouani, H, Correia, S, da Costa, PM, Paccheco, R, Zarazaga, M, Ruiz-Larrea, F, Poeta, P, Igrejas, G. 2010. Vancomycin-resistant enterococci from Portuguese wastewater treatment plants. Journal of Basic Microbiology 50: 605-609. IF=1.395.


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16. Arnaud-Haond, S, Marbà, N, Diaz-Almela, E, Serrão, EA, Duarte, CM. 2010. Comparative analysis of stability - genetic diversity in seagrass (Posidonia oceanica) meadows yields unexpected results. Estuaries and Coasts 33: 878-889. IF=1.921.

17. Athanasiadis, A, Neto, AI. 2010. On the occurrence of Mesophyllum expansum (Philippi) Cabioch et Mendoza (Melobesioideae, Corallinales, Rhodophyta) in the Mediterranean, the Canary Isles and the Azores. Botanica Marina 53: 333-341. IF=1.623.

18. Auamcharoen, W, Chandrapatya, A, Naengchomnong, W, Kijjoa, A. 2010. A new tetralone from Diospyros cauliflora. Planta Medica 76: 1207. IF=2.369.

19. Azevedo, C, Casal, G, Mendonça, I, Carvalho, E, Matos, P, Matos, E. 2010. Light and electron microscopy of Myxobolus sciades n. sp. (Myxozoa), a parasite of the gills of the Brazilian fish Sciades herzbergii (Block, 1794) (Teleostei: Ariidae). Memórias Instituto Oswaldo Cruz 105(2): 203-207. IF=2.058.

20. Azevedo, C, Marques, DKS, Casal, G, Amaral, CMC, Silva, EV, Matos, P, Matos, E. 2010. Ultrastructural re-description of Henneguya piaractus (Myxozoa), a parasite of the freshwater fish Piaractus mesopotamicus (Teleostei, Characidae) from the Paraguay river, Brazil. Acta Protozoologica 49: 115-120. IF=0.881.

21. Azevedo, C, Samuel, N, Saveia, A, Delgado, F, Casal, G. 2010. Light and electron microscopic data of spore of Thelohanellus rhabdalestus n. sp. (Myxozoa: Myxosporea), a parasite of a freshwater fish from Kwanza River of Angola. Systematic Parasitology 79: 19-25. IF=1.056.

22. Azevedo, IC, Bordalo, AA, Duarte, P. 2010. Influence of river discharge patterns on the hydrodynamics and potential contaminant dispersion in the Douro estuary (Portugal). Water Research 44: 3133-3146. IF=4.546.

23. Azevedo, IC, Duarte, P, Bordalo, AA. 2010. Temporal and spatial variability of phytoplankton photosynthetic characteristics in a southern European estuary (Douro, Portugal). Marine Ecology Progress Series 412: 29-44. IF=2.483.

24. Azul, AM, Ramos, V, Sales, F. 2010. Early effects of fire on herbaceous vegetation and mycorrhizal symbiosis in high altitude grasslands of Natural Park of Estrela Mountain (PNSE). Symbiosis 52: 113-123. IF=1.442.

25. Balata, D, Piazzi, L, Nesti, U, Bulleri, F, Bertocci, I. 2010. Effects of enhanced loads of nutrients on epiphytes on leaves and rhizomes of Posidonia oceanica. Journal of Sea Research 63: 173-179. IF=2.444.

26. Barrento, S, Marques, A, Teixeira, B, Mendes, R, Bandarra, N, Vaz-Pires, P, Nunes, ML. 2010. Chemical composition, cholesterol, fatty acid and amino acid in two populations of brown crab Cancer pagurus: Ecological and human health implications. Journal of Food Composition and Analysis 23(7): 716-725. IF=1.948.

27. Barrento, S, Marques, A, Vaz-Pires, P, Nunes, ML. 2010. Live shipment of immersed crabs Cancer pagurus from England to Portugal and recovery in stocking tanks: stress parameter characterization. ICES Journal of Marine Science 67: 435-443. IF=1.808.

28. Batini, C, Lopes, J, Behar, DM, Calafell, F, Jorde, LB, van der Veen, L, Quintana-Murci, L, Spedini, G, Destro-Bisol, G, Comas, D. 2010. Insights into demographic history of african pygmies from complete mitochondrial genomes. Molecular Biology and Evolution 28: 1099-1110. IF=5.510.


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29. Becheler, R, Diekmann, O, Hily, C, Moalic, Y, Arnaud-Haond, S. 2010. The concept of population in clonal organisms: mosaics of temporally colonized patches are forming highly diverse meadows of Zostera marina in Brittany. Molecular Ecology 19: 2394-2407. IF=6.457.

30. Behrends, T, Urbatzka, R, Krackow, S, Elepfandt, A, Kloas, W. 2010. Mate calling behavior of male South African clawed frogs (Xenopus laevis) is suppressed by the antiandrogenic endocrine disrupting compound flutamide. General and Comparative Endocrinology 168: 269-274. IF=3.108.

31. Bensimon-Brito, A, Cancela, ML, Huysseune, A, Witten, PE. 2010. The zebrafish (Danio rerio) caudal complex - a model to study vertebral body fusion. Journal of Applied Ichthyology 26(2): 235-238. IF=0.945.

32. Bertocci, I, Arenas, F, Matias, M, Vaselli, S, Araújo, R, Abreu, H, Pereira, R, Vieira, R, Sousa-Pinto, I. 2010. Canopy-forming species mediate the effects of disturbance on macroalgal assemblages on Portuguese rocky shores. Marine Ecology Progress Series 414: 107-116. IF=2.483.

33. Bertocci, I, Maggi, E, Vaselli, S, Benedetti-Cecchi, L. 2010. Resistance of rocky shore assemblages of algae and invertebrates to changes in intensity and temporal variability of aerial exposure. Marine Ecology Progress Series 400: 75-86. IF=2.483.

34. Billard, E, Serrão, EA, Pearson, GA, Destombe, C, Valero, M. 2010. Fucus vesiculosus and spiralis species complex: a nested model of local adaptation at the shore level. Marine Ecology Progress Series 405: 163-174. IF=2.483.

35. Blanquet, I, Oliva-Teles, A. 2010. The effect of feed restriction on performance of turbot (Scophthalmus maximus) juveniles under commercial rearing conditions. Aquaculture Research 41: 1255-1260. IF=1.186.

36. Borrero-Pérez, GH, Gómez-Zurita, J, González-Wangüemert, M, Marcos, C, Pérez-Ruzafa, A. 2010. Molecular systematics of the genus Holothuria in the Mediterranean and Northeastern Atlantic and a molecular clock for the diversification of the Holothuriidae (Echinodermata: Holothuroidea). Molecular Phylogenetics and Evolution 57: 899-906. IF=3.889.

37. Bos, MS, Bastos, L, Fernandes, RMS. 2010. The influence of seasonal signals on the estimation of the tectonic motion in short continuous GPS time-series. Journal of Geodynamics 49(3-4): 205-209. IF=1.197.

38. Bos, MS. 2010. Comment on “Anomalous ocean load tide signal observed in lake-level variations in Tierra del Fuego” by A. Richter et al. Geophysical Research Letters 36: L04303. IF=3.505.

39. Bulleri, F, Balata, D, Bertocci, I, Tamburello, L, Benedetti-Cecchi, L. 2010. The seaweed Caulerpa racemosa on Mediterranean rocky reefs: from passenger to driver of ecological change. Ecology 91: 2205-2212. IF=5.073.

40. Cabaço, S, Ferreira, Ó, Santos, R. 2010. Population dynamics of the seagrass Cymodocea nodosa in Ria Formosa lagoon following inlet artificial relocation. Estuarine Coastal and Shelf Science 87: 510-516. IF=1.887.

41. Cabaço, S, Santos. R. 2010. Reproduction of the eelgrass Zostera marina at the species southern distributional limit in the Eastern Atlantic. Marine Ecology – An Evolutionary Prespective 31: 300-308. IF=1.272.


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42. Cabral, JP. 2010. Can we use indoor fungi as bioindicators of indoor air quality? Historical perspectives and open questions. Science of the Total Environment 408: 4285-4295. IF=3.190.

43. Cabral, JP. 2010. La revista Broteria, los jesuitas naturalistas y Gonçalo Sampaio. Intercambio de plantas e ideas y el desarrollo de la botánica en Portugal. Asclepio - Revista de Historia de la Medicina y de la Ciencia 62(1): 61-92.

44. Cabral, JP. 2010. Water microbiology. Bacterial pathogens and water. International Journal of Environmental Research and Public Health 7: 3657-3703.

45. Cabrita, E, Sarasquete, C, Martínez-Páramo, S, Robles, V, Beirão, J, Pérez-Cerezales, S, Herráez, MP. 2010. Cryopreservation of fish sperm: applications and perspectives. Journal of Applied Ichthyology 26: 623-635. IF=0.945.

46. Calado, R, Pimentel, T, Cleary, D, Dionísio, G, Nunes, C, Lopes da Silva, T, Dinis, MT, Reis, A. 2010. Providing a common diet to different marine decapods does not standardize the fatty acid profiles of their larvae: a warning sign for experimentation using invertebrate larvae produced in captivity. Marine Biology 157: 2427-2434. IF=2.011.

47. Campinho, MA, Galay-Burgos, M, Sweeney, GE, Power, DM. 2010. Coordination of deiodinase and thyroid hormone receptor expression during the larval to juvenile transition in sea bream (Sparus aurata, Linnaeus). General and Comparative Endocrinology 165(2): 181-194. IF=3.108.

48. Campos, A, Carvajal-Vallejos, PK, Villalobos, E, Franco, CF, Almeida, AM, Coelho, AV, Torné, JM, Santos, M. 2010. Characterisation of Zea mays L. plastidial transglutaminase: interactions with thylakoid membrane proteins. Plant Biology 12: 708-716. IF=2.409.

49. Campos, A, Vasconcelos, VM. 2010. Molecular Mechanisms of Microcystin Toxicity in Animals. International Journal of Molecular Sciences 11: 268-287. IF=2.279.

50. Campos, C, Valente, LMP, Borges, P, Bizuayehu, ST, Fernandes, JMO. 2010. Dietary lipid levels have a remarkable impact on the expression of growth-related genes in Senegalese sole (Solea senegalensis Kaup). Journal of Experimental Biology 213: 200-209. IF=3.040.

51. Campos, J, Bio, A, Cardoso, JFMF, Dapper, R, Witte, J IJ, Van der Veer, HW. 2010. Fluctuations of brown shrimp Crangon crangon abundance in the western Dutch Wadden Sea. Marine Ecology Progress Series 405: 203-219. IF=2.483.

52. Cancela, ML, Witten, PE. 2010. Advances in skeletal biology by understanding the fish skeleton: a multidisciplinary challenge. Journal of Applied Ichthyology 26(2): 147-147. IF=0.945.

53. Canning-Clode, J, Maloney, KO, Mcmahon, SM, Wahl, M. 2010. Expanded view of the local-regional richness relationship by incorporating functional richness and time: a large-scale perspective. Global Ecology and Biogeography 19: 875-885. IF=5.273.

54. Cardoso, C, Mendes, R, Pedro, S, Vaz-Pires, P, Nunes, M. 2010. Quality changes during storage of minced fish products containing dietary fibre and fortified with ω3 fatty acids. Food Science and Technology International 16(1): 131-142. IF=0.577.

55. Cardoso, C, Mendes, R, Vaz-Pires, P, Nunes, ML. 2010. Effect of salt and MTGase on the production of high quality gels from farmed sea bass. Journal of Food Engineering 101: 98-105. IF=2.168.


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56. Cardoso, CL, Mendes, RO, Salvador, AM, Saraiva, JA, Vaz-Pires, P, Nunes, ML. 2010. Quality characteristics of high pressure-induced hake (Merluccius capensis) protein gels with and without MTGase. Journal of Aquatic Food Product Technology 19: 193-213. IF=0.481.

57. Cardoso, JCR, Vieira, FA, Gomes, AS, Power, DM. 2010. The serendipitous origin of chordate secretin peptide family members. BMC Evolutionary Biology 10: 135. IF=3.702.

58. Carvalho, PN, Basto, MCP, Silva, MFGM, Machado, A, Bordalo, A, Vasconcelos, MTSD. 2010. Ability of salt marsh plants for TBT remediation in sediments. Environmental Science and Pollution Research 17: 1279-1286. IF=2.870.

59. Casal, G, Teles-Grilo, ML, Matos, E, Azevedo, C. 2010. Ultrastructural and molecular characterization of a new Microsporidium parasite from the Amazonian fish, Gymnorhamphichthys rondoni (Rhamphichthyidae). Journal of Parasitology 96: 1155-1163. IF=1.208.

60. Cavaleiro, FI, Santos, MJ, Ho, J-S. 2010. Caligus musaicus n. sp. (Copepoda: Caligidae) parasitic on the flounder Platichthys flesus (Linnaeus) off Portugal. Crustaceana 83 (4): 457-464. IF=0.630.

61. Cermeno, P, de Vargas, C, Abrantes, F, Falkowski, PG. 2010. Phytoplankton biogeography and community stability in the Ocean. Plos One 5(3): e10037. IF=4.411.

62. Chew, SF, Tng, YY, Wee, NL, Tok, CY, Wilson, JM, Ip, YK. 2010. Intestinal osmoregulatory acclimation and nitrogen metabolism in juveniles of the freshwater marble goby exposed to seawater. Journal of Comparative Physiology B 180: 511-520. IF=2.400.

63. Clark, MS, Thorne, MAS, Vieira, FA, Cardoso, JCR, Power, DM, Peck, LS. 2010. Insights into shell deposition in the Antarctic bivalve Laternula elliptica: gene discovery in the mantle transcriptome using 454 pyrosequencing. BMC Genomics 11: 362. IF=4.206.

64. Claudet, J, Osenberg, CW, Domenici, P, Badalamenti, F, Milazzo, M, Falcón, JM, Bertocci, I, Benedetti-Cecchi, L, García-Charton, J-A, Goñi, R, Borg, JA, Forcada, A, de Lucia, GA, Pérez-Ruzafa, Á, Afonso, P, Brito, A, Guala, I, Le Diréach, L, SanchezJerez, P, Somerfield, PJ, Planes, S. 2010. Marine reserves: fish life history and ecological traits matter. Ecological Applications 20: 830-839. IF=4.276.

65. Coelho, R, Bentes, L, Correia, C, Gonçalves, JMS, Lino, PG, Monteiro, P, Ribeiro, J, Erzini, K. 2010. Life history of the common pandora, Pagellus erythrinus (Linnaeus, 1758) (Actinopterygii: Sparidae) from southern Portugal. Brazilian Journal of Oceanography 58: 233-245. IF=0.266.

66. Coelho, R, Rey, J, Gil de Sola, L, Fernandez-Carvalho, J, Erzini, K. 2010. Comparing NE Atlantic and Mediterranean populations of a deep water lanternshark, with comments on the efficiency of density dependant compensatory mechanisms. Marine Biology Research 6: 373-380. IF=1.484.

67. Coelho, R. 2010. [Book Review]: Biology and Management of Dogfish Sharks, Edited by V.F. Gallucci, G.A. McFarlane & G.G. Bargmann. Marine Biology Research 6: 415-416. IF=1.484.

68. Coelho, R., Erzini, K. 2010. Depth distribution of the velvet belly, Etmopterus spinax, in relation to growth and reproductive cycle: The case study of a deep water lantern shark with a wide ranging critical habitat. Marine Biology Research 6: 381-389. IF=1.484.

http://www.ncbi.nlm.nih.gov/pubmed/20024567




260

69. Conceicão, LEC, Aragão, C, Richard, N, Engrola, S, Gavaia, P, Mira, S, Dias, J. 2010. Novel methodologies in marine fish larval nutrition. Fish Physiology and Biochemistry 36: 1-16. IF=1.607.

70. Conceicão, LEC, Yúfera, M, Makridis, P, Morais, S, Dinis, MT. 2010. Live feeds for early stages of fish rearing. Aquaculture Research 36: 1-16. IF=1.186.

71. Conceicao, N, Cox, CJ, Simões, B, Viegas, M, Cancela, ML. 2010. Comparative promoter analysis and its application to the identification of candidate regulatory factors of cartilage-expressed genes. Journal of Applied Ichthyology 26(2): 245-250. IF=0.945.

72. Costa-Dias, S, Dias, E, Lobon-Cervia, J, Antunes, C, Coimbra J. 2010. Infection by Anguillicoloides crassus in a riverine stock of European eel, Anguilla anguilla. Fisheries Management and Ecology 17: 485-492. IF=0.798.

73. Costa-Dias, S, Freitas, V, Sousa, R, Antunes, C. 2010. Factors influencing epibenthic assemblages in the Minho estuary (NW Iberian Peninsula). Marine Pollution Bulletin 61: 240-246. IF=2.359.

74. Costa-Dias, S, Sousa, R, Antunes, C. 2010. Ecological quality assessment of the lower Lima estuary. Marine Pollution Bulletin 61: 234-239. IF=2.359.

75. Couto, MNPFS, Monteiro, E, Vasconcelos, MTSD. 2010. Mesocosms trials of bioremediation of contaminated soil of a petroleum refinery: comparison of natural attenuation, biostimulation and bioaugmentation. Environmental Science and Pollution Research 17: 1339-1346. IF=2.870.

76. Couto, RP, Neto, AI, Rodrigues, AS. 2010. Metal concentration and structural changes in Corallina elongata (Corallinales, Rhodophyta) from hydrothermal vents. Marine Pollution Bulletin 60: 509-514. IF=2.359.

77. Cox, CJ, Goffinet, B, Wickett, NJ, Boles, SB, Shaw, AJ. 2010. Moss diversity: a molecular phylogenetic analysis of genera. Phytotaxa 9: 175-195.

78. Cravo, A, Relvas, P, Cardeira, S, Rita, F, Madureira, M, Sánchez, R. 2010. An upwelling filament off Southwest Iberia: effect on the chlorophyll a and nutrients exportation. Continental Shelf Research 30: 1601-1613. IF=1.928.

79. Creer, S, Fonseca, VG, Porazinska, DL, Giblin-Davis, RM, Sung, W, Power, DM, Packer, M, Carvalho, GR, Blaxter, ML, Lambshead, PJD, Thomas, WK. 2010. Ultrasequencing of the meiofaunal biosphere: practice, pitfalls and promises. Molecular Ecology 19: 4-20. IF=6.457.

80. da Fonseca, RR, Johnson, WE, O’Brien, SJ, Vasconcelos, V, Antunes, A. 2010. Molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases. BMC Evolutionary Biology 10: 281. IF=3.702.

81. da Fonseca, RR, Kosiol, C, Vinar, T, Siepel, A, Nielsen, R. 2010. Positive selection on apoptosis related genes. FEBS Letters 584: 469-476. IF=3.601.

82. Dâmaso-Rodrigues, ML, Pousão-Ferreira, P, Ribeiro, L, Coutinho, J, Bandarra, NM, Gavaia, PJ, Narciso, L, Morais, S. 2010. Lack of essential fatty acids in live feed during larval and post larval rearing: effect on the performance of juvenile Solea senegalensis. Aquaculture International 18(5): 741-757. IF=0.880.


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83. Davies, SJ, Gouveia, A. 2010. Response of common carp fed diets containing an isogenic pea seed meal (Pisum sativum) subjected to different thermal processing methods. Aquaculture 305(1-4): 117-123. IF=2.044.

84. Delgado, C, Canário, AVM, Dellinger, T. 2010. Sex ratios of loggerhead sea turtles Caretta caretta during the juvenile pelagic stage. Marine Biology 157: 979-990. IF=2.011.

85. Dias, J, Yúfera, M, Valente, LMP, Rema, P. 2010. Feed transit and apparent protein, phosphorus and energy digestibility of practical feed ingredients by Senegalese sole (Solea senegalensis). Aquaculture 302: 94-99. IF=2.044.

86. Diekmann, OE, Gouveia, L, Perez, JA, Gil-Rodriguez, C, Serrao, EA. 2010. The possible origin of Zostera noltii in the Canary Islands and guidelines for restoration. Marine Biology 157: 2109-2115. IF=2.011.

87. Dini-Andreote, F, Andreote, FD, Costa, R, Taketani, RG, van Elsas, JD, Araújo, WL. 2010. Bacterial soil community in a Brazilian sugarcane field. Plant and Soil 336: 337-349. IF=2.773.

88. Diogo, P, Soares, F, Dinis, MT, Cabrita, E. 2010. The influence of ovarian fluid on Solea senegalensis sperm motility. Journal of Applied Ichthyology 26: 690-695. IF=0.945.

89. Dolbeth, M, Martinho, F, Freitas, V, Costa-Dias, S, Campos, J, Pardal, MA. 2010. Multi-year comparisons of fish recruitment, growth and production in two drought-affected Iberian estuaries. Marine and Freshwater Research 61: 1399-1415. IF=1.572.

90. Duarte, P, Fernández-Reiriz, MJ, Filgueira, RA, Labarta, U. 2010. Modelling mussel growth in ecosystems with low suspended matter loads. Journal of Sea Research 64: 273-286. IF=2.444.

91. Eiras, JC, Monteiro, CM, Brasil-Sato, MC. 2010. Myxobolus franciscoi sp. nov. (Myxozoa, Myxobolidae), a parasite of Prochilodus argenteus (Actynopterygii, Prochilodontidae) from the Upper São Francisco River, Brazil, with a revision of Myxobolus spp. from South America. Zoologia 27: 131-137. IF=0.373.

92. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, Del Campo, F, Vasconcelos, V, Oudra, B. 2010. Effects of the microcystin profile of a bloom on the toxicity and toxin accumulation in common carp (Cyprinus carpio L.) larvae. Journal of Fish Biology 76:1415-1430. IF=1.330.

93. El Ghazali, I, Saqrane, S, Carvalho, AP, Ouahid, Y, del Campo, FF, Oudra, B, Vasconcelos, V. 2010. Effect of different microcystin profiles on the toxins bioaccumulation in common carp (Cyprinus carpio) larvae via Artemia nauplii. Ecotoxicology and Environmental Safety 73:762-770. IF=2.340.

94. Enes, P, Peres, H, Couto, A, Oliva-Teles, A 2010. Growth performance and metabolic utilization of diets including starch, dextrin, maltose or glucose as carbohydrate source by gilthead sea bream (Sparus aurata) juveniles. Fish Physiology and Biochemistry 36: 903-910. IF=1.607.

95. Enes, P, Sanchez-Gurmaches, J, Navarro, I, Gutiérrez, J, Oliva-Teles, A. 2010. Role of insulin and IGF-I on the regulation of glucose metabolism in European sea bass (Dicentrarchus labrax) fed with different dietary carbohydrate levels. Comparative Biochemistry and Physiology 157A: 346-353. IF=2.134.

96. Engrola, S, Dinis, MT, Conceição, LEC. 2010. Senegalese sole larvae growth and protein utilization is depressed when co-fed high levels of inert diet and Artemia since first feeding. Aquaculture Nutrition 16: 457-465. IF=1.393.


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97. Erzini, K, Gonçalves, JMS, Bentes, L, Lino, PG, Ribeiro, J, Moutopoulos, DK, Stergiou, KI. 2010. Competition between static gears of the small-scale fisheries in Algarve waters (southern Portugal). Mediterranean Marine Science 11(2): 225-243. IF=0.568.

98. Fathalli, A, Jenhani, A, Saker, M, Moreira, C, Romdhane, MS, Vasconcelos, VM. 2010. First observation of the toxic and invasive cyanobacterium species Cylindrospermopsis raciborskii in Tunisian freshwaters. Toxicity assessment and molecular characterization. Fresenius Environmental Bulletin 19: 1074-1083. IF=0.716.

99. Fazenda, C, Simões, B, Kelsh, RN, Cancela, ML, Conceição, N. 2010. Dual transcriptional regulation by runx2 of matrix Gla protein in Xenopus laevis. Gene 450(1-2): 94-102. IF=2.266.

100. Fernandes, MJ, Lázaro, C, Nunes, AL, Pires, N, Bastos, L, Mendes, VB. 2010. GNSS-derived Path Delay: an approach to compute the wet tropospheric correction for coastal altimetry. IEEE Geoscience and Remote Sensing Letters 7(3): 596-600. IF=1.431.

101. Ferraresso, S, Milan, M, Pellizzari, C, Vitulo, N, Reinhardt, R, Canario, AVM, Patarnello, T, Bargelloni, L. 2010. Development of an oligo DNA microarray for the European sea bass and its application to expression profiling of jaw deformity. BMC Genomics 11: 354. IF=4.206.

102. Ferreira, F, Santos, MM, Reis-Henriques, MA, Vieira, NM, Monteiro, NM. 2010. Sexing blennies using genital papilla morphology or anogenital distance. Journal of Fish Biology 77: 1432-1438. IF=1.330.

103. Ferreira, M, Caetano, M, Antunes, P, Costa, J, Gil, O, Bandarra, N, Pousão-Ferreira, P, Vale, C, Reis-Henriques, MA. 2010. Assessment of contaminants and biomarkers of exposure in wild and farmed seabass. Ecotoxicology and Environmental Safety 73: 579-588. IF=2.340.

104. Figueiredo-Silva, AC, Corraze, G, Borges, P, Valente, LMP. 2010. Dietary protein/lipid level and protein source effects on growth, tissue composition and lipid metabolism of Blackspot seabream (Pagellus bogaraveo). Aquaculture Nutrition 16: 173-187. IF=1.393.

105. Figueiredo-Silva, AC, Corraze, G, Kaushik, S, Peleteiro, JB, Valente, LMP. 2010. Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids. Amino Acids 39: 1401-1416. IF=4.106.

106. Figueiredo-Silva, AC, Corraze, G, Valente, LMP. 2010. The growth and nutrient utilisation of blackspot seabream (Pagellus bogaraveo) under different feeding regimes. Fish Physiology and Biochemistry 36: 1113-1124. IF=1.607.

107. Fletcher, WJ, Sanchez-Goni, MF, Allen, JRM, Cheddadi, R, Combourieu-Nebout, N, Huntley, B, Lawson, I, Londeix, L, Magri, D, Margari, V, Mueller, UC, Naughton, F, Novenko, E, Roucoux, K, Tzedakis, PC. 2010. Millennial-scale variability during the last glacial in vegetation records from Europe. Quaternary Science Reviews 29: 2839-2864. IF=4.657.

108. Flores, J-A, Colmenero-Hidalgo, E, Mejia-Molina, AE, Baumann, K-H, Henderiks, J, Larsson, K, Prabhu, CN, Sierro, FJ, Rodrigues, T. 2010. Distribution of large Emiliania huxleyi specimens in the Central and Northeast Atlantic during the last 25,000 years and its utility to monitor the onset of warm conditions. Marine Micropaleontology 76: 53-66. IF=2.321.

109. Fonseca, VG, Carvalho, GR, Sung, W, Johnson, HF, Power, DM, Neill, SP, Packer, M, Blaxter, ML, Lambshead, PJD, Thomas, WK, Creer, S. 2010. Second-generation environmental sequencing unmasks marine metazoan biodiversity. Nature Communications 1: 98.


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110. Francisco, C, Almeida, A, Castro, A, Santos, MJ. 2010. Development of a PCR-RFLP marker to genetically distinguish Prosorhynchus crucibulum and Prosorhynchus aculeatus. Parasitology International 59: 40-34. IF=2.259.

111. Francisco, C, Hermida, M, Santos, MJ. 2010. Parasites and Symbionts from Mytilus galloprovincialis (Lamark, 1819) (Bivalves: Mytilidae) of the Aveiro Estuary Portugal. Journal of Parasitology 96(1): 200-205. IF=1.208.

112. Frasco, MF, Erzen, I, Stojan, J, Guilhermino, L. 2010. Localization and properties of cholinesterases in the common prawn (Palaemon serratus): a kinetic-histochemical study. Biological Bulletin 218: 1-5. IF=2.475.

113. Frazão, B, Martins, R, Vasconcelos, V. 2010. Are know toxins involved in the toxicity of picoplanktonic and filamentous North Atlantic marine cyanobacteria?. Marine Drugs 8: 1908-1919. IF=3.471.

114. Freedman, H, Martel, P, Cruzeiro, L. 2010. Mixed quantum-classical dynamics of an amide I vibrational excitation in a protein α-helix. Physical Review B 82: 174308. IF=3.774.

115. Freitas, V, Campos, J, Skreslet, S, Van der Veer, HW. 2010. Habitat quality of a subarctic nursery ground for 0-group plaice (Pleuronectes platessa L.). Journal of Sea Research 64: 26-33. IF=2.444.

116. Freitas, V, Cardoso, JFMF, Lika, K, Peck, MA, Campos, J, Kooijman, SALM, Van der Veer, HW. 2010. Temperature tolerance and energetics: a dynamic energy budget-based comparison of North Atlantic marine species. Philosophical Transactions of the Royal Society B 365: 3553-3565. IF=6.053.

117. Frija, LMT, Ismael, A, Cristiano, MLS. 2010. Photochemical transformations of tetrazole derivatives: applications in organic synthesis. Molecules 15(5): 3757-3774. IF=1.988.

118. Fu, C, Wilson, JM, Rombough, P, Brauner, CJ. 2010. Ions first: Naþ uptake shifts from the skin to the gills before O2 uptake in developing rainbow trout, Oncorhynchus mykiss. Proceedings of the Royal Society B 277: 1553-1560. IF=5.064.

119. Fuentes, J, Brinca, L, Guerreiro, PM, Power, DM. 2010. PRL and GH synthesis and release from the sea bream (Sparus auratus L.) pituitary gland in vitro in response to osmotic challenge. General and Comparative Endocrinology 168(1): 95-102. IF=3.108.

120. Fuentes, J, Power, DM, Canario, AV. 2010. Parathyroid hormone-related protein-stanniocalcin antagonism in regulation of bicarbonate secretion and calcium precipitation in a marine fish intestine. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 299(1): R150-158. IF=3.284.

121. Gabriel, D, Parente, MI, Neto, AI, Raposo, M, Schils, T, Fredericq, S. 2010. Phylogenetic appraisal of the genus Platoma (Nemastomatales, Rhodophyta), including life history and morphological observations on P. cyclocolpum from the Azores. Phycologia 49 (1): 2-21. IF=2.080.

122. Gibbons, P, Verissimo, E, Araujo, NC, Barton, V, Nixon, GL, Amewu, RK, Chadwick, J, Stocks, PA, Biagini, GA, Srivastava, A, Rosenthal, PJ, Gut, J, Guedes, RC, Moreira, R, Sharma, R, Berry, N, Cristiano, MLS, Shone, AE, Ward, SA, O’Neill, PM. 2010. Endoperoxide carbonyl falcipain 2/3 inhibitor hybrids; towards combination chemotherapy of malaria through a single chemical entity. Journal of Medicinal Chemistry 53: 8202-8206. IF=5.207.


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124. Gomes, NCM, Flocco, CG, Costa, R, Junca, H, Vilchez, R, et al. 2010. Mangrove microniches determine the structural and functional diversity of enriched petroleum hydrocarbon-degrading consortia. FEMS Microbiology Ecology 74: 276-290. IF=3.456.

125. Gómez-Requeni, P, Conceição, LEC, Olderbakk Jordal, A, Rønnestad, I. 2010. A reference growth curve for nutritional experiments in zebrafish (Danio rerio) and changes in whole body proteome during development. Fish Physiology and Biochemistry 36: 1199-1215. IF=1.607.

126. Goncalves, D, Saraiva, J, Teles, M, Teodosio, R, Canario, AVM, Oliveira, RF. 2010. Brain aromatase mRNA expression in two populations of the peacock blenny Salaria pavo with divergent mating systems. Hormones and Behavior 57(2): 155-161. IF=3.991.

127. Gonçalves, JFM, Turini, BGS, Ozório, ROA. 2010. The effects of stocking densities on performance of turbot (Scophthalmus maximus) fed diets with L-carnitine supplementation. Scientia Agricola 67(2): 151-157. IF=0.816.

128. González-Díaz, H, Dea-Ayuela, MA, Pérez-Montoto, LG, Prado-Prado, FJ, Agüero-Chapín, G, Bolas-Fernández, F, Vazquez-Padrón, RI, Ubeira, FM. 2010. QSAR for RNases and theoretic-experimental study of molecular diversity on peptide mass fingerprints of a new Leishmania infantum protein. Molecular Diversity 14: 349-369. IF=3.721.

129. González-Wangüemert, M, Cánovas, F, Pérez-Ruzafa, A, Marcos, C, Alexandrino, P. 2010. Connectivity patterns inferred from the genetic structure of white seabream (Diplodus sargus L.). Journal of Experimental Marine Biology and Ecology 383: 23-31. IF=1.910.

130. Granja, H, Rocha, F, Matias, M, Moura, R, Caldas, F, Marques, J, Tareco, H. 2010. Lagoa da Apúlia: a residual lagoon from Late Holocene (NW coastal zone of Portugal). Quaternary International 221: 46-57. IF=1.768.

131. Gravato, C, Guimarães, L, Santos, J, Faria, M, Alves, A, Guilhermino, L. 2010. Comparative study about the effects of pollution on glass and yellow eels (Anguilla anguilla) from the estuaries of Minho, Lima and Douro Rivers (NW Portugal). Ecotoxicology and Environmental Safety 73: 524-533. IF=2.340.

132. Groisillier, A, Herve, C, Jeudy, A, Rebuffet, E, Pluchon, PF, Chevolot, Y, Flament, D, Geslin, C, Morgado, IM, Power, DM, Branno, M, Moreau, H, Michel, G, Boyen, C, Czjzek, M. 2010. MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms. Microbial Cell Factories 9: 45. IF=4.544.

133. Guedes, AC, Meireles, LA, Amaro, HM, Malcata, FX. 2010. Changes in lipid class and fatty acid composition of cultures of Pavlova lutheri, in response to light intensity. Journal of the American Oil Chemists Society 87(7): 791-801. IF=1.587.

134. Guerreiro, PM, Canario, AVM, Power, DM, Renfro, JL. 2010. Piscine PTHrP regulation of calcium and phosphate transport in winter flounder renal proximal tubule primary cultures. American Journal of Physiology – Regulatory, Integrative and Comparative Physiology 299: R603-R611. IF=3.284.

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135. Guilhermino, L, Guimarães, L. 2010. Are complex approaches a real advantage in the assessment of ecotoxicological effects in real scenarios? Comparative Biochemistry and Physiology A 157: S19-S20. IF=2.134.

136. Guimarães, L, Gravato, C, Santos, J, Guilhermino, L. 2010. Impact of pollution on the development of glass eels (Anguilla anguilla) entering three NW Portuguese estuaries. Comparative Biochemistry and Physiology A - Molecular and Integrative Physiology 157: S25. IF=2.134.

137. Herfindal, L, Jokela, J, Myhren, L, Permi, P, Selheim, F, Wahlsten, M, Kleppe, R, Krakstad, C, Vasconcelos, V, Sivonen, K, Døskeland, SO. 2010. A novel nostocyclopeptide inhibits microcystin and nodularin induced apoptosis in hepatocytes. CHEMBIOCHEM 11: 1594-1599. IF=3.945.

138. Hermelink, B, Urbatzka, R, Wiegand, C, Pflugmacher, S, Lutz, I, Kloas, W. 2010. Aqueous leaf extracts display endocrine activities in vitro and disrupt sexual differentiation of male Xenopus laevis tadpoles in vivo. General and Comparative Endocrinology 168: 245-255. IF=3.108.

139. Hernández-Garcia, A, Romero, D, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Effects on enzymatic antioxidant system of mallard (Anas platyrhynchos) erythrocytes exposed in vitro to lead and cadmium. Toxicology Letters 196: 128-129. IF=3.581.

140. Hernández-Garcia, A, Romero, D, Tagliati, CA, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Glutathione S-transferase activity, glutathione and lipid peroxidation levels in mallard (Anas platyrhynchos) erythrocytes exposed to lead and cadmium in vitro. Toxicology Letters 196: 128. IF=3.581.

141. Honrado, J, Vicente, J, Lomba, A, Alves, P, Macedo, JA, Henriques, R, Granja, H, Caldas, FB. 2010. Fine-scale patterns of vegetation assembly in the monitoring of changes in coastal sand-dune landscapes. Web Ecology 10: 1-14.

142. Hormiga, JA, Almansa, E, Sykes, AV, Torres, N. 2010. Model based optimization of feeding regimens in aquaculture: Application to the improvement of Octopus vulgaris paralarvae viability in captivity. Journal of Biotechnology 149: 209-214. IF=2.970.

143. Huertas, M, Hagey, L, Hofmann, AF, Cerda, J, Canario, AVM, Hubbard, PC. 2010. Olfactory sensitivity to bile fluid and bile salts in the European eel (Anguilla anguilla), goldfish (Carassius auratus) and Mozambique tilapia (Oreochromis mossambicus) suggests a 'broad range' sensitivity not confined to those produced by conspecifics alone. Journal of Experimental Biology 213(2): 308-317. IF=3.040.

144. Ibarz, A, Costa, R, Harrison, AP, Power, DM. 2010. Dietary keto-acid feed-back on pituitary activity in gilthead sea bream: Effects of oral doses of AKG. A proteomic approach. General and Comparative Endocrinology 169(3): 284-292. IF=3.108.

145. Iglesias, T, Lobon-Cervia, J, Dias, SC, Antunes, C. 2010. Variation of life traits of glass eels of Anguilla anguilla (L.) during the colonization of Rios Naln and Minho estuaries (northwestern Iberian Peninsula). Hydrobiologia 651(1): 213-223. IF=1.964.

146. Incera, M, Bertocci, I, Benedetti-Cecchi, L. 2010. Effects of mean intensity and temporal variability of disturbance on the invasion of Caulerpa racemosa var. cylindracea (Caulerpales) in rock pools. Biological Invasions 12: 501-514. IF=3.474.

147. Ismael, A, Cristiano, MLS, Fausto, R, Gomez-Zavaglia, A. 2010. Tautomer selective


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148. Johnstone, RAW, Loureiro, RMS, Cristiano, MLS, Labat, G. 2010. Bond Energy/Bond Order relationships for N-O linkages and a quantitative measure of ionicity: the role of nitro groups in hydrogen bonding. ARKIVOC 2010(v): 142-169. IF=1.096.

149. King, MA, Altamimi, Z, Boehm, J, Bos, M, Dach, R, Elosegui, P, Fund, F, Hernandez-Pajares, M, Lavallee, D, Cerveira, PJM, Penna, N, Riva, REM, Steigenberger, P, van Dam, T, Vittuari, L, Williams, S, Willis, P. 2010. Improved constraints on models of glacial isostatic adjustment: A review of the contribution of ground-based geodetic observations. Surveys in Geophysics 31(5): 465-507. IF=3.590.

150. Kjellström, E, Brandefelt, J, Naeslund, JO, Smith, B, Strandberg, G, Voelker, AHL, Wohlfarth, B. 2010. Simulated climate conditions in Europe during the Marine Isotope Stage 3 stadial. BOREAS 39: 436-456. IF=3.052.

151. Konar, B, Iken, K, Cruz-Motta, JJ, Benedetti-Cecchi, L, Knowlton, A, Pohle, G, Miloslavich, P, Edwards, M, Trott, T, Kimani, E, Riosmena-Rodriguez, R, Wong, M, Jenkins, S, Mead, A, Silva, A, Sousa Pinto, I, Shirayama, Y. 2010. Global patterns of macroalgal diversity and biomass in rocky nearshore environments. Plos One 5: 13195. IF=4.411.

152. Kopecka-Pilarczyk, J, Coimbra, J. 2010. Short term high hydrostatic pressure effect on selected biomarkers in silver eel (Anguilla anguilla). Journal of Experimental Marine Biology and Ecology 391: 118-124. IF=1.910.

153. Kopecka-Pilarczyk, J, Coimbra, J. 2010. The effect of elevated hydrostatic pressure upon selected biomarkers in juvenile blackspot seabream Pagellus bogaraveo in a 14 day-long experiment. Brief communication. Journal of Fish Biology 77: 279-284. IF=1.330.

154. Kopecka-Pilarczyk, J. 2010. Effect of exposure to polycyclic aromatic hydrocarbons on Na+/K

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155. Kopecka-Pilarczyk, J. 2010. In vitro effects of pesticides and metals on the activity of acetylcholinesterase (AChE) from different tissues of the blue mussel, Mytilus trossulus L. Journal of Environmental Science and Health B: Pesticides, Food Contaminants, and Agricultural Wastes 45(1): 46-52. IF=1.119.

156. Kopecka-Pilarczyk, J. 2010. The effect of pesticides and metals on acetylcholinesterase (AChE) in various tissues of blue mussel (Mytilus trossulus L.) in short-term in vivo exposures at different temperatures. Journal of Environmental Science and Health B: Pesticides, Food Contaminants, and Agricultural Wastes 45(4): 336-346. IF=1.119.

157. Korn, M, Green, AJ, Machado, M, García-de-Lomas, J, Cristo, M, Cancela da Fonseca, L, Frisch, D, Pérez-Bote, JL, Hundsdoerfer, AK. 2010. Phylogeny, molecular ecology and taxonomy of southern Iberian lineages of Triops mauritanicus (Crustacea: Notostraca). Organisms Diversity & Evolution 10(5): 409-440. IF=1.581.

158. Kuhl, H, Beck, A, Wozniak, G, Canario, AVM, Volckaert, FAM, Reinhardt, R. 2010. The European sea bass Dicentrarchus labrax genome puzzle: comparative BAC-mapping and low coverage shotgun sequencing. BMC Genomics 11: 68. IF=4.206.


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159. Kumar, A, Ergas, S, Yuan, X, Sahu, A, Zhang, QO, Dewulf, J, Malcata, FX, van Langenhove, H. 2010. Enhanced CO2 fixation and biofuel production via microalgae: recent developments and future directions. Trends in Biotechnology 28(7): 371-380. IF=9.644.

160. Lago-Leston, A, Mota, C, Kautsky, L, Pearson, GA. 2010. Functional divergence in heat shock response following rapid speciation of Fucus spp. in the Baltic Sea. Marine Biology 157: 683-688. IF=2.011.

161. Leão, PN, Pereira, AR, Liu, W-T, König, GM, Dorrestein, PC, Vasconcelos, VM, Gerwick, WH. 2010.

Synergistic allelochemicals from a freshwater cyanobacterium. Proceedings of the National Academy of Sciences of the United States of America 107: 11183-11188. IF=9.771.

162. Leitão, A, Carvalho, S, Ben-Hamadou, R, Gaspar, MB. 2010. Cytogenetics of Hediste diversicolor (Annelida: Polychaeta) and comparative karyological analysis within Nereididae. Aquatic Biology 10: 193-200. IF=1.611.

163. Lobo-da-Cunha, A, Oliveira, E, Alves, A, Coelho, R, Calado, G. 2010. Light and electron microscopic study of the anterior oesophagus of Bulla striata (Mollusca, Opisthobranchia). Acta Zoologica 91:125-138. IF=1.130.

164. Lobo-da-Cunha, A, Oliveira, E, Ferreira, I, Coelho, R, Calado, G. 2010. Histochemical and ultrastructural characterization of the posterior esophagus of Bulla striata (Mollusca, Opisthobranchia). Microscopy and Microanalysis 16: 688-698. IF=3.259.

165. Lopes, A, Lopes-Lima, M, Bobos, I, Ferreira, J, Gomes, S, Reis, R, Mano, J, Machado, J. 2010. The effects of Anodonta cygnea biological fluids on biomineralization of chitosan membranes. Journal of Membrane Science 364: 82-89. IF=3.673.

166. Lopes, C, Abrantes, F, Mix, AC. 2010. Environmental controls of diatoms species in North-east Pacific sediments. Palaeogeography, Palaeoclimatology, Palaeoecology 297: 188-200. IF=2.390.

167. Lopes, V, Antunes, A, Martins, R, Welker, M, Vasconcelos, V. 2010. Morphological, toxicological and molecular characterization of a benthic Nodularia strain isolated from a Portuguese estuary. Research in Microbiology 161: 9-17. IF=2.405.

168. Lopes, V, Fernandez, N, Martins, R, Vasconcelos, VM. 2010. Primary screening for bioactivity of brackishwater cyanobacteria: Ecotoxicity and embryotoxicity of crude extracts. Marine Drugs 8: 471-482. IF=3.471.

169. Lopes-Lima, M, Rocha, A, Goncalves, F, Andrade, J, Machado, J. 2010. Microstructural characterization of inner shell layers in the freshwater bivalve Anodonta cygnea. Journal of Shellfish Research 29: 969-973. IF=1.041.

170. Louro, B, Hadjipavlou, G, Hemani, G, Leach, R, Nadaf, J, Rowe, S, de Koning, DJ. 2009. Extensive QTL and Association Analyses of the QTLMAS2009 Data. 13th QTL-MAS Workshop. BMC Proceedings 4 (Suppl 1): S11.

171. Louro, B, Passos, ALS, Souche, E, Tsigenopoulos, C, Beck, A, Bonhomme, F, Cancela, L, Cerdà, J, Clark, MS, Kotoulas, G, Lubzens, E, Planas, J, Volckaert, FAM, Reinhardt, R, Canario, AVM. 2010. Gilthead sea bream (Sparus auratus) and European sea bass (Dicentrarchus labrax) expressed sequence tags: characterization, tissue specific expression and gene markers. Marine Genomics 3(3-4): 179-191. IF=0.841.


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172. Macedo, JA, Alves, P, Lomba, A, Vicente, J, Hespanhol, H, Henriques, R, Granja, H, Honrado, J. 2010. On the interest of plant functional classifications to study community-level effects of increased disturbance on coastal dune vegetation. Acta Botanica Gallica 157 (2): 305-315. IF=0.298.

173. Madeira, S, Gonçalves, JA, Bastos, L. 2010. Photogrammetric mapping and measuring application using MATLAB. Computers & Geosciences 36(6): 699-706. IF=1.416.

174. Madureira, TV, Barreiro, JC, Rocha, MJ, Rocha, E, Cass, QB, Tiritan, ME. 2010. Spatio-temporal distribution of pharmaceuticals in the Douro River estuary (Portugal): A study using liquid chromatography ion trap mass spectrometry. Science of the Total Environment 408: 5513-5520. IF=3.190.

175. Madureira, TV, Rocha, MJ, Cass, QB, Tiritan, ME. 2010. Development and optimization of a HPLC-DAD method for the determination of diverse pharmaceuticals in estuarine surface waters. Journal of Chromatographic Science 48: 176-182. IF=1.078.

176. Makridis, P, Libeiro, L, Rocha, R, Dinis MT. 2010. Influence of microalgae supernatant, and bacteria isolated from microalgae cultures, on microbiology, and digestive capacity of larval gilthead seabream, Sparus aurata, and Senegalese Sole, Solea senegalensis. Journal of the World Aquaculture Society 41: 780-790. IF=0.839.

177. Marçalo, A, Marques, TA, Araújo, J, Pousão-Ferreira, P, Erzini, K, Stratoudakis, Y. 2010. Fishing simulation experiments for predicting the effects of purse-seine capture on sardine (Sardina pilchardus). ICES Journal of Marine Science 67: 334-344. IF=1.808.

178. Marques, JF, Santos, MJ, Cabral, HN. 2010. Aggregation patterns of macroendoparasites in phylogenetically related fish hosts. Parasitology 137(11): 1671-1680. IF=2.522.

179. Marques, JRF, da Fonseca, RR, Drury, B, Melo, A. 2010. Conformational characterization of disulfide bonds: A tool for protein classification. Journal of Theoretical Biology 267: 388-395. IF=2.371.

180. Marques, JRF, da Fonseca, RR, Drury, B, Melo, A. 2010. Amino acid patterns around disulfide bonds. International Journal of Molecular Sciences 11(11): 4673-4686. IF=2.279.

181. Marques-da-Silva, D, Samhan-Arias, AK, Tiago, T, Gutierrez-Merino, C. 2010. L-type calcium channels and cytochrome b(5) reductase are components of protein complexes tightly associated with lipid rafts microdomains of the neuronal plasma membrane. Journal of Proteomics 73: 1502-1510. IF=5.074.

182. Martínez-López, E, Sousa, AR, María-Mojica, P, Gómez-Ramirez, P, Guilhermino, L, García-Fernández, AJ. 2010. Blood delta-ALAD, lead and cadmium concentrations in spur-thighed tortoises (Testudo graeca) from Southeastern Spain and Northern Africa. Ecotoxicology 19: 670-677. IF=3.051.

183. Martins da Costa, P, Bica, A, Vaz-Pires, P, Bernardo, F. 2010. Changes in antimicrobial resistance among faecal enterococci isolated from growing broilers prophylactically medicated with three commercial antimicrobials. Preventive Veterinary Medicine 93: 71-76. IF=2.070.

184. Martins, CIM, Eding, EH, Verdegem, MCJ, Heinsbroek, LTN, Schneider, O, Blancheton, JP, d'Orbcastel, ER, Verreth, JAJ. 2010. New developments in recirculating aquaculture systems in

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185. Martins, GM, Thompson, RC, Neto, AI, Hawkins, SJ, Jenkins, SR. 2010. Enhancing stocks of the exploited limpet Patella candei via modifications in coastal engineering. Biological Conservation 143: 203-211. IF=3.498.

186. Martins, GM, Thompson, RC, Neto, AI, Hawkins, SJ, Jenkins, SR. 2010. Exploitation of intertidal grazers as a driver of community divergence. Journal of Applied Ecology 47: 1282-1289. IF=4.970.

187. Martins, J, Peixe, L, Vasconcelos, V. 2010. Cyanobacteria and Bacteria co-occurrence in a Wastewater Treatment Plant (WWTP): Absence of Allelopathic Effects. Water, Science and Technology 62(8): 1954-1962. IF=1.056.

188. Martins, M, Faleiro, ML, Chaves, S, Tenreiro, R, Costa, MC. 2010. Effect of uranium (VI) on two sulphate-reducing bacteria cultures from a uranium mine site. Science of the Total Environment 408: 2621-2628. IF=3.190.

189. Martins, M, Faleiro, ML, Chaves, S, Tenreiro, R, Santos, ES, Costa, MC. 2010. Anaerobic bio-removal of uranium (VI) and chromium (VI): Comparison of microbial community structure. Journal of Hazardous Materials 176: 1065-1072. IF=3.723.

190. Martins, M, Faleiro, ML, Costa, AMR, Chaves, S, Tenreiro, R, Matos, AP, Costa, MC. 2010.

Mechanism of uranium (VI) removal by two anaerobic bacterial communities. Journal of Hazardous Materials 184: 89-96. IF=3.723.

191. Martins, M, Santos, ES, Pires, C, Barros, RJ, Costa, MC. 2010. Production of irrigation water from

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192. Massari, A, Urbatzka, R, Cevasco, A, Canesi, L, Lanza, C, Scarabelli, L, Kloas, W, Mandich, A. 2010. Aromatase mRNA expression in the brain of adult Xenopus laevis exposed to Lambro river water and endocrine disrupting compounds. General and Comparative Endocrinology 168: 262-268. IF=3.108.

193. Massault, C, Hellemans, B, Louro, B, Batargias, C, Van Houdt, JKJ, Canario, AVM, Volckaert, FAM, Bovenhuis, H, Haley, C, de Koning, DJ. 2010. QTL for body weight, morphometric traits and stress response in European sea bass Dicentrarchus labrax. Animal Genetics 41(4): 337-345. IF=2.203.

194. Mata, L, Schuenhoff, A, Santos R. 2010. A direct comparison of two seaweed biofilters: Asparagopsis armata and Ulva rigida. Journal of Applied Phycology 22: 639-644. IF=1.792.

195. Matos, E, Gonçalves, A, Nunes, ML, Dinis, MT, Dias, J. 2010. Effect of harvesting stress and slaughter conditions on selected flesh quality criteria of gilthead seabream (Sparus aurata). Aquaculture 305: 66-72. IF=2.044.

196. McCarthya, AL, Heppella, S, Royerb, F, Freitas, C, Dellinger, D. 2010. Identification of likely foraging habitat of pelagic loggerhead sea turtles (Caretta caretta) in the North Atlantic through analysis of telemetry track sinuosity. Progress in Oceanography 86: 224-231. IF=3.269.

197. McGuire, A, Aluru, N, Takemura, A, Weil, R, Wilson, JM, Vijayan, MM. 2010. Osmotic shock adaptation by cortisol involves the suppression of gill metabolic capacity in Mozambique Tilapia. General and Comparative Endocrinology 165: 321-329. IF=3.108.


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199. Medeiros, PR, Souza, AT, Ilarri, MI. 2010. Habitat use and behavioural ecology of the juveniles of two sympatric damselfishes (Actinopterygii: Pomacentridae) in the south-western Atlantic Ocean. Journal of Fish Biology 77(7): 1599-1615. IF=1.330.

200. Mendonca, VM, Al Saady, S, Al Kiyumi, A, Erzini, K. 2010. Interactions between green turtles (Chelonia mydas) and foxes (Vulpes vulpes arabica, V. rueppellii sabaea, and V. cana) on turtle nesting grounds in the Northwestern Indian Ocean: Impacts of the fox community on the behavior of nesting sea turtles at the Ras Al Hadd Turtle Reserve, Oman. Zoological Studies 49: 437-452. IF=1.046.

201. Menezes, S, Soares, AMVM, Guilhermino, L, Peck, MR. 2010. Can the activities of acetylcholinesterase and glutathione S-transferases of Crangon crangon (L.) be used as biomarkers of fuel oil exposure? Water, Air and Soil Pollution 208: 317-322. IF=1.765.

202. Mesquita, SR, Guilhermino, L, Guimarães, L. 2010. Assessing the effects of the selective serotonin reuptake inhibitor fluoxetine on Carcinus maenas using locomotor behaviour and biomarkers as effect criteria. Comparative Biochemistry and Physiology A 157: S23. IF=2.134.

203. Milanin, T, Eiras, JC, Arana, S, Maia, AAM, Alves, AL, Silva, MRM, Carriero, MM, Ceccarelli, PS, Adriano, EA. 2010. Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai n. sp., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil. Memórias Instituto Oswaldo Cruz 105: 762-769. IF=2.058.

204. Mil-Homens, M, Costa, A, Lebreiro, S, Canário, J, Lopes, C, Mouro, F, Mateus, M, Stigter, H, Richter, T, Branco, V, Trancoso, M, Melo, Z, Boer, W. 2010. Temporal clustering of metals in a short sediment core of the Cascais Canyon (Portuguese Margin). Scientia Marina 74(S1): 89-98. IF=1.079.

205. Monteiro, P, Bentes, L, Coelho, R, Correia, C, Erzini, K, Lino, PG, Ribeiro, J, Gonçalves, JMS. 2010. Age and growth, mortality and reproduction of the striped sea bream, Lithognathus mormyrus Linnaeus 1758, from the south coast of Portugal (Algarve). Marine Biology Research 6: 53-65. IF=1.484.

206. Morais, P, Babaluk, J, Correia, AT, Chícharo, MA, Campbell, JL, Chícharo, L. 2010. Diversity of anchovy migration patterns in an European temperate estuary and in its adjacent coastal area: Implications for fishery management. Journal of Sea Research 64: 295-303. IF=2.444.

207. Moreira, J, Veiga, P, Rubal, M. 2010. First record of Pisione guanche (Polychaeta: Pisionidae) at the Azores Archipielago. Marine Biodiversity Records 3: 1-6.

208. Moreira, SM, Moreira-Santos, M, Rendón-von Osten, J, da silva, EM, Ribeiro, R, Guilhermino, L, Soares, AM. 2010. Ecotoxicological tools for the tropics: Sublethal assays with fish to evaluate edge-of-field pesticide runoff toxicity. Ecotoxicology and Environmental Safety 73: 893-899. IF=2.340.

209. Moreira-Silva, J, Tsui, TKN, Coimbra, J, Vijayan, MM, Ip, YK, Wilson, JM. 2010. Branchial ammonia excretion in the Asian weatherloach Misgurnus anguillicaudatus. Comparative Biochemistry and Physiology C – Toxicology & Pharmacology 151(1): 40-50. IF=2.325.

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210. Mota, A, Guedes, F, Areias, J, Pinho, L, Cardoso, MF. 2010. Alcohol consumption among patients with hepatitis B infection in northern Portugal considering gender and hepatitis B virus genotype differences. Alcohol 44(2): 149-156. IF=2.423.

211. Mourato, BL, Coelho, R, Amorim, AF, Carvalho, F, Hazin, FHV, Burgess, G. 2010. Size at maturity and length-weight relationships of the blurred lantern shark Etmopterus bigelowi (Squaliformes: Etmopteridae), caught off southeastern Brazil. Ciencias Marinas 36: 323-331. IF=0.618.

212. Mucha, AP, Almeida, CMR, Bordalo, A, Vasconcelos, MTSD. 2010. LMWOA (low molecular weight organic acid) exudation by salt marsh plants: Natural variation and response to Cu contamination. Estuarine, Coastal and Shelf Science 88: 63-70. IF=1.887.

213. Neiva, J, Pearson, GA, Valero, M, Serrao, EA. 2010. Surfing the wave on a borrowed board: range expansion and spread of introgressed organellar genomes in the seaweed Fucus ceranoides L. Molecular Ecology 19: 4812-4822. IF=6.457.

214. Nicastro, KR, Zardi, GI, McQuaid, CD, Stephens, L, Radloff, S, Blatch, GL. 2010. The role of gaping behaviour in habitat partitioning between coexisting intertidal mussels. BMC Ecology 10: 17.

215. Nicastro, KR, Zardi, GI, McQuaid, CD. 2010. Differential reproductive investment, attachment strength and mortality of invasive and indigenous mussels across heterogeneous environments. Biological Invasions 12: 2165-2177. IF=3.474.

216. Nogueira, I, Lobo da Cunha, AL, Afonso, A, Rivera, S, Azevedo, J, Monteiro, R, Cervantes, R, Gago-Martinez, A, Vasconcelos, V. 2010. Toxic effects of domoic acid in the sea bream Sparus aurata. Marine Drugs 8: 2721-2732. IF=3.471.

217. Nogueira, N, Cordeiro, N, Canada, P, Cruz e Silva, P, Ozorio, ROA. 2010. Separate and combined effects of cyclic fasting and l-carnitine supplementation in red porgy (Pagrus pagrus, L. 1978). Aquaculture Research 41(11): e795-e806. IF=1.186.

218. Oliva, M, de Canales, MLG, Gravato, C, Guilhermino, L, Perales, JA. 2010. Biochemical effects and polycyclic aromatic hydrocarbons (PAHs) in senegal sole (Solea senegalensis) from a Huelva estuary (SW Spain). Ecotoxicology and Environmental Safety 73: 1842-1851. IF=2.340.

219. Oliveira, M, Vieira, LR, Gravato, C, Guilhermino, L. 2010. Acute effects of pyrene on the common goby Pomatoschistus microps (Teleostei, Gobiidae). Toxicology Letters 196: 127-128. IF=3.581.

220. Ozório, ROA, Turini, BGS, Moro, G, Oliveira, LST, Portz, L, Cyrino, JEP. 2010. Growth, nitrogen gain and indispensable amino acid retention of pacu (Piaractus mesopotamicus, Holmberg 1887) fed different brewers yeast (Saccharomyces cerevisiae) levels. Aquaculture Nutrition 16: 276-283. IF=1.393.

221. Ozorio, ROA, Van Ginneken, VJT, Bessa, RJB, Verstegen, MWA, Verreth, JAJ, Huisman, EA. 2010. Effects of exercise on L-carnitine and lipid metabolism in African catfish (Clarias gariepinus) fed different dietary L-carnitine and lipid levels. British Journal of Nutrition 103: 1139-1150. IF=3.072.

222. Parente, MI, Fletcher, RL, Neto, AI, Tittley, I, Sousa, AF, Draisma, S, Gabriel, D. 2010. Life history and morphological studies of Punctaria tenuissima (Chordariaceae, Phaeophyceae), a new record for the Azores. Botanica Marina 53 (3): 223-231. IF=1.623.


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223. Pereira, AM, Robalo, JI, Freyhof, J, Maia, C, Fonseca, JP, Valente, A, Almada, VC. 2010. Phylogeographical analysis reveals multiple conservation units in brook lampreys Lampetra planeri of Portuguese streams. Journal of Fish Biology 77(2): 361-371. IF=1.330.

224. Pearson, GA, Hoarau, G, Lago-Leston, A, Coyer, JA, Kube, M, Henckel, K, Serrão, EA, Corre, E, Stam, WT, Olsen, JL. 2010. An expressed sequence Tag (EST) analysis of the intertidal brown seaweeds Fucus serratus (L.) and F. vesiculosus (L.) (Heterokontophyta, Phaeophyceae) in response to abiotic stressors. Marine Biotechnology 12: 195-213. IF=2.962.

225. Pereira, MJ, Palming, J, Svensson, MK, Aureliano, M Eriksson, J. 2010. Immunosuppresive agents alter insulin signalling and glucose and lipid metabolism in human subcutaneous adipocytes. Diabetologia 53: 710. IF=6.973.

226. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2010. Evaluation of DNA damage as a quality marker for rainbow trout sperm cryopreservation and use of LDL as cryoprotectant. Theriogenology 74: 282-289. IF=2.045.

227. Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Herráez, MP. 2010. Fertilization capacity with rainbow trout DNA damaged sperm and embryo developmental success. Reproduction 139: 1-10. IF=3.049.

228. Pinheiro, PLC, Cardoso, JCR, Gomes, AS, Fuentes, J, Power, DM, Canário, AVM. 2010. Gene structure, transcripts and calciotropic effects of the PTH family of peptides in Xenopus and chicken. BMC Evolutionary Biology 10: 373. IF=3.702.

229. Pinto, L, Radhouani, L, Coelho, C, Martins da Costa, P, Simões, R, Brandão, RML, Torres, C, Igrejas, G and Poeta, P. 2010. Genetic detection of extended-spectrum {beta}-lactamases Escherichia coli in birds of prey from Serra da Estrela Natural Reserve of Portugal. Applied and Environmental Microbiology 76(12): 4118-20. IF=3.778.

230. Pinto, PI, Matsumura, H, Thorne, MA, Power, DM, Terauchi, R, Reinhardt, R, Canario, AVM. 2010. Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish. BMC Genomics 11: 476. IF=4.206.

231. Pinto, W, Figueira, L, Ribeiro, L, Yúfera, M, Dinis, MT, Aragão, C. 2010. Dietary taurine supplementation enhances metamorphosis and growth potential of Solea senegalensis larvae. Aquaculture 309: 159-164. IF=2.044.

232. Pinto, W, Rodrigues, V, Dinis, MT, Aragão, C. 2010. Can dietary aromatic amino acid supplementation be beneficial during fish metamorphosis? Aquaculture 310: 200-205. IF=2.044.

233. Poinar, G, Duarte, D, Santos, MJ. 2010. Halomonhystera parasitica n. sp. (Nematoda: Monhysteridae), a parasite of Talorchestia brito (Crustacea: Talitridae) in Portugal. Systematic Parasitology 75: 53-58. IF=1.056.

234. Power, DM, Fuentes, J, Harrison, AP. 2010. A non-invasive monitoring device for anesthetics in fish. Open Access Animal Physiology 2: 1-7.

235. Queiroz, CA, Kijjoa, A, David, JM, David, JP. Flavonoids and benzoic acid derivatives from Dioclea virgata. Planta Medica 76: 1284. IF=2.369.


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236. Rafael, MS, Marques, CL, Parameswaran, V, Cancela, ML, Laizé, V. 2010. Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology. Journal of Applied Ichthyology 26: 230-234. IF=0.945.

237. Ramos, S, Moura, JJG, Aureliano, M. 2010. Actin as a potential target for decavanadate. Journal of Inorganic Biochemistry 104: 1234-1239. IF=3.317.

238. Ramos, S, Moura, JJG, Aureliano, M. Decavanadate effects in actin structure and function. FEBS Journal 277: 190. IF=3.129.

239. Ramos, S, Ré, P, Bordalo, AA. 2010. Recruitment of flatfish species to an estuarine nursery habitat (Lima estuary, NW Iberian Peninsula). Journal of Sea Research 64: 473-486. IF=2.444.

240. Ramos, V, Seabra, R, Brito, Â, Santos, A, Santos, CL, Lopo, M, Moradas-Ferreira, P, Vasconcelos, V, Tamagnini, P. 2010. Characterization of a marine unicellular cyanobacterium that constitutes a separate clade together with thermophilic strains. European Journal of Phycology 44: 394-403. IF=1.901.

241. Ramos, V, Vasconcelos, V. 2010. Palytoxin and analogues: biological and ecological effects. Marine Drugs 8(7): 2021-2037. IF=3.471.

242. Redruello, B, Louro, B, Anjos, L, Silva, N, Greenwell, RS, Canario, AVM, Power, DM. 2010. CRTAC1 homolog proteins are conserved from cyanobacteria to man and secreted by the teleost fish pituitary gland. Gene 456(1-2): 1-14. IF=2.266.

243. Resende, AD, Lobo-da-Cunha, A, Malhão, F, Franquinho, F, Monteiro, RAF, Rocha, E. 2010. Histological and stereological characterization of brown trout (Salmo trutta) trunk kidney. Microscopy and Microanalysis 16: 677-687. IF=3.259.

244. Rey, J, Coelho, R, Lloris, D, Séret, B, Gil de Sola, L. 2010. Distribution pattern of Galeus atlanticus in the Alborán Sea (south western Mediterranean) and some sexual character comparison with Galeus melastomus. Marine Biology Research 6: 364-372. IF=1.484.

245. Rocha, E, Rocha, MJ, Lobo-da-Cunha, A, Galante, MH, Monteiro, RAF. 2010. The hepatocytes of the brown trout (Salmo trutta f. fario): A stereological study of some cytoplasmic components during the breeding cycle. Microscopy Research and Technique 73: 766-778. IF=1.721.

246. Rodrigues, T, Grimalt, JO, Abrantes, F, Naughton, F, Flores, J-A. 2010. The last glacial-interglacial transition (LGIT) in the western mid-latitudes of the North Atlantic: Abrupt sea surface temperature change and sea level implications. Quaternary Science Reviews 29: 1853-1862. IF=4.657.

247. Rogerson, M, Colmenero-Hidalgo, E, Levine, RC, Rohling, EJ, Voelker, AHL, Bigg, GR, Schönfeld, J, Cacho, I, Sierro, FJ, Loewemark, L, Reguera, MI, de Abreu, L, Garrick, K. 2010. Enhanced Mediterranean-Atlantic exchange during Atlantic freshening phases. Geochemistry, Geophysics, Geosystems 11(8): Q08013. IF=3.368.

248. Romano, L, Eiras, JC, Raibenberg, FC, Alvares, M, Saraiva, A, Cruz, C. 2010. A case of ceroid deposition in the kidney of feral rainbow trout, Oncorhynchus mykiss, in Alicura impoundment, Argentina. Bulletin of the European Association of Fish Pathologists 30: 25-29. IF=0.414.


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249. Rosa, J, Tiago, DM, Dias, J, Cancela, ML, Laizé, V. 2010. Serum-related stimulation of the proliferation and mineralization of seabream bone-derived cells. Journal of Applied Ichthyology 26: 245-250. IF=0.945.

250. Rosas-Alquicira, EF, Riosmena-Rodríguez, R, Neto, AI. 2010. The typification and status of Amphiroa cryptarthrodia (Corallinales, Rhodophyta). Phycological Research 58: 248-257. IF=1.186.

251. Salgueiro, E, Voelker, AHL, de Abreu, L, Abrantes, F, Meggers, H, Wefer, G. 2010. Temperature and Productivity Changes off the Western Iberian Margin during the last 150 ky. Quaternary Science Reviews 29: 680-695. IF=4.657.

252. Santos, MJ, Cavaleiro, F, Campos, P, Sousa, A, Teixeira, F, Martins, M. 2010. Impact of amoeba and scuticociliatidia infections on the aquaculture European sea bass (Dicentrarchus labrax L.) in Portugal. Veterinary Parasitology 171: 15-21. IF=2.331.

253. Santos, MM, Solé, M, Lima, D, Hambach, B, Ferreira, AM, Reis-Henriques, MA. 2010. Validating a multi-biomarker approach with the shanny Lipophrys pholis to monitor oil spills in European marine ecosystems. Chemosphere 81: 685-691. IF=3.155.

254. Sarrico, CS, Rosa, MJ, Teixeira, PN, Cardoso, MF. 2010. Assessing quality and evaluating performance in higher education: worlds apart or complementary views?. Minerva 48(1): 35-54.

255. Schander, C, Rapp, HT, Kongsrud, JA, Bakken, T, Berge, J, Cochrane, S, Oug, E, Byrkjedal, I, Todt, C, Cedhagen, T, Fosshagen, A, Gebruk, A, Larsen, K, Levin, L, Obst, M, Pleijel, F, Stöhr, S, Warén, A, Mikkelsen, NT, Hadler-Jacobsen, S, Keuning, R, Petersen, KH, Thorseth, IH, Pedersen, RB. 2010. The fauna of hydrothermal vents on the Mohn Ridge (North Atlantic). Marine Biology Research 6(2): 155-171. IF=1.484.

256. Seixas, P, Otero, A, Valente, LMP, Dias, J, Rey-Méndez, M. 2010. Growth and fatty acid composition of Octopus vulgaris paralarvae fed with enriched Artemia or co-fed with an inert diet. Aquaculture International 18: 1121-1135. IF=0.880.

257. Seixas, P, Rey-Méndez, M, Valente, LMP, Otero, A. 2010. High DHA content in Artemia is ineffective to improve Octopus vulgaris paralarvae rearing. Aquaculture 300: 156-162. IF=2.044.

258. Serra, IA, Innocenti, AM, Di Maida, G, Calvo, S, Migliaccio, M, Zambianchi, E, Pizzigalli, C, Arnaud-Haond, S, Duarte, CM, Serrão, EA, Procaccini, G. 2010. Genetic structure in the Mediterranean seagrass Posidonia oceanica. Disentangling past vicariance events from contemporary patterns of gene flow. Molecular Ecology 19: 557-568. IF=6.457.

259. Shaw, AJ, Cox, CJ, Buck, WR, Devos, N, Buchanan, AM, Cave, L, Seppelt, R, Shaw, B, Larran, J, Andrus, R, Greilhuber, J, Temsch, EM. 2010. Newly resolved relationships in an early land plant lineage: Bryophyta class Sphagnopsida (peat mosses). American Journal of Botany 97: 1511-1531. IF=3.052.

260. Shaw, AJ, Devos, N, Cox, CJ, Boles, SB, Shaw, B, Buchanan, AM, Cave, L, Seppelt, R. 2010. Peatmoss (Sphagnum) diversification associated with Miocene Northern Hemisphere climatic cooling?. Molecular Phylogenetics and Evolution 55: 1139-1145. IF=3.889.

261. Silva, JMG, Espe, M, Conceição, LEC, Dias, J, Costas, B, Valente, LMP. 2010. Feed intake and growth of Senegalese sole (Solea senegalensis, Kaup 1858) fed diets with partial replacement of fish meal with plant proteins. Aquaculture Research 41: e20-e30. IF=1.186.


275

262. Silva K, Vieira, MN, Almada, VC, Monteiro, NM. 2010. Female reproductive tactics: Scanning for male quality and number. Behavioral Ecology 20: 768-772. IF=2.926.

263. Silva, K, Vieira, MN, Almada, VC, Monteiro, NM. 2010. Reversing sex-role reversal competes only when you must. Animal Behaviour 79(4): 885-893. IF=3.101.

264. Silva, P, Power, DM, Valente, LMP, Silva, N, Monteiro, RAF, Rocha, E. 2010. Expression of the

myosin light chains 1,2 and 3 in the muscle of blackspot seabream, Pagellus bogaraveo, during development. Fish Physiology and Biochemistry 36: 1125-1132. IF=1.607.

265. Silva, P, Vasconcelos, V. 2010. Allelopathic effect of water contaminated with Cylindrospermopsis raciborskii on the germination and growth of several plant species. Chemistry and Ecology 26: 263-271. IF=0.776.

266. Silva, PIM, Martins, CIM, Engrola, S, Marino, G, Øverli, Ø, Conceicão, LEC. 2010. Individual differences in cortisol levels and behaviour of Senegalese sole (Solea senegalensis) juveniles: evidences for coping styles. Applied Animal Behaviour Science 124: 75-81. IF=1.555.

267. Silva, TS, Cordeiro, O, Richard, N, Conceição, LEC, Rodrigues, PM. 2010. Changes in the soluble bone proteome of reared white seabream (Diplodus sargus) with skeletal deformities. Comparative Biochemistry and Physiology - Part D 6: 82-91. IF=1.800.

268. Simão, MF, Leite, RB, Rocha, C, Cancela, ML. 2010. Changes in bioturbation of iron biogeochemistry and in molecular response of the clam Ruditapes decussates upon Perkinsus olseni infection. Archives of Environmental Contamination and Toxicology 59: 433-443. IF=1.930.

269. Simões, R, Poirel, L, Martins da Costa, P, Nordmann, P. 2010. Seagulls and beaches as a reservoir for emerging extended spectrum beta-lactamase producers in Escherichia coli. Emerging Infectious Diseases 16(1): 110-112. IF=6.859.

270. Sousa, JJ, Ruiz, AM, Hanssen, RF, Bastos, L, Gil, AJ, Galindo-Zaldívar, J. 2010. PS-InSAR processing methodologies in the detection of field surface deformation – study of the Granada basin (Central Betic Cordilleras, Southern Spain). Journal of Geodynamics 49: 181-189. IF=1.197.

271. Sousa, P, Froufe, E, Alves, PC, Harris, DJ. 2010. Genetic diversity within Scorpions of the genus Buthus from the Iberian Peninsula: mitochondrial DNA sequence data indicate additional distinct cryptic lineages. Journal of Arachnology 38(2): 206-211. IF=0.901.

272. Stoichev, T, Basto, MCP, Vasconcelos, V, Vasconcelos, MTSD. 2010. Fate and effects of nonylphenol in the presence of the cyanobacterium Microcystis aeruginosa. Chemistry and Ecology 26: 395-399. IF=0.776.

273. Swiderski, Z, Gibson, DI, Santos, MJ, Poddubnaya, LG. 2010. Ultrastructure of the intrauterine eggs of Didymobothrium rudolphii (Monticelli, 1890) (Cestoda, Spathebothriidea, Acrobothriidae) and its phylogenetic implications. Acta Parasitologica 55(3): 254-269. IF=1.144.

274. Teixeira, C, Magalhães, C, Boaventura, RAR, Bordalo, AA. 2010. Potential rates and environmental controls of denitrification and nitrous oxide production in temperate urbanized estuary. Marine Environmental Research 70: 336-342. IF=1.953.

275. Teixeira, S, Cambon-Bonavita, M, Serrao, EA, Desbruyeres, D, Arnaud-Haond, S. 2010. Recent population expansion and connectivity in the hydrothermal shrimp Rimicaris exoculata along the Mid Atlantic Ridge. Journal of Biogeography 38: 564-574. IF=4.273.


276

276. Thomsen, MS, Wernberg, T, Altieri, A, Tuya, F, Gulbransen, D, McGlathery, K, Holmerk, M, Silliman, BR. 2010. Facilitation cascades via habitat formation and modification. Integrative and Comparative Biology 50:158-175. IF=2.626.

277. Thomsen, MS, Wernberg, T, Tuya, F, Silliman, BR. 2010. Ecological performance and possible origin of a ubiquitous but under-studied gastropod. Estuarine Coastal and Shelf Science 87: 501-509. IF=1.887.

278. Tiago, T, Palma, PS, Gutierrez-Merino, C, Aureliano, M. 2010. Peroxynitrite-mediated oxidative modifications of myosin and implications on structure and function. Free Radical Research 44: 1317-1327. IF=2.805.

279. Trubiroha, A, Kroupova, H, Wuertz, S, Frank, SN, Sures, B, Kloas, W. 2010. Naturally-induced endocrine disruption by the parasite Ligula intestinalis (Cestoda) in roach (Rutilus rutilus).General and Comparative Endocrinology 166: 234-240. IF=3.108.

280. Tuya, F, Larsen, K, Platt, V. 2010. Patterns of abundance and assemblage structure of epifauna inhabiting two morphologically different kelp holdfasts. Hydrobiologia 658(1): 373-382. IF=1.964.

281. Tuya, F, Vanderklift, MA, Hyndes, GA, Wernberg, T, Thomsen, MS, Hanson, C. 2010. Proximity to reefs affects the balance between positive and negative effects on seagrass fauna. Marine Ecology Progress Series 405: 175-186. IF=2.483.

282. Urbatzka, R, Lorenz, C, Lutz, I, Kloas, W. 2010. Expression profiles of LH beta, FSH beta and their gonadal receptor mRNAs during sexual differentiation of Xenopus laevis tadpoles. General and Comparative Endocrinology 168: 239-244. IF=3.108.

283. Valente, LMP, Olmedo, M, Álvarez-Blázquez, B, Borges, P, Soares, S, Gomes, EFS, Pazos, G, Linares, F. 2010. Dietary protein and carbohydrate source on growth, body composition and tissue lipid deposition of blackspot seabream, Pagellus bogaraveo (Brunnich). Animal Physiology and Animal Nutrition 94: 212-219. IF=1.106.

284. van der Veer, HW, Freitas, V, Koot, J, White, JIJ, Zuur, AF. 2010. Food limitation in epibenthic species in temperate intertidal systems in summer: analysis of 0-group plaice Pleuronectes platessa. Marine Ecology Progress Series 416: 215-227. IF=2.483.

285. Van Elsas, JD, Semenov, A, Costa, R, Trevors, J. 2011. Survival of Escherichia coli in the environment: fundamental and public health aspects. ISME Journal 5: 367. IF=6.153.

286. Vandepitte, L, Vanhoorne, B, Kraberg, A, Anisimova, N, Antoniadou, C, Araújo, R, Bartsch, I, Beker, B, Benedetti-Cecchi, L, Bertocci, I, Cochrane, SJ, Cooper, KM, Craeymeersch, JA, Christou, E, Crisp, DJ, Dahle, S, De Boissier, M, de Kluijver, M, Denisenko, S, De Vito, D, Duineveld, G, Escaravage, V, Fleischer, D, Fraschetti, S, Giangrande, A, Heip, CHR, Hummel, H, Janas, U, Karez, R, Kedra, M, Kingston, P, Kuhlenkamp, R, Libes, M, Martens, P, Mees, J, Mieszkowska, N, Mudrak, S, Munda, I, Orfanidis, S, Orlando-Bonaca, M, Palerud, R, Rachor, E, Reichert, K, Rumohr, H, Schiedek, D, Schubert, P, Sistermans, WCH, Pinto, IS, Southward, AJ, Terlizzi, A, Tsiaga, E, van Beusekom, JEE. 2010. Data integration for European marine biodiversity research: creating a database on benthos and plankton to study large-scale patterns and long-term changes. Hydrobiologia 644: 1-13. IF=1.964.

287. Vasconcelos, R, Froufe, E, Brito, JC, Carranza, S, Harris, DJ. 2010. Phylogeography of Amietophrynus regularis based on mitochondrial DNA sequences: inferences regarding the Cape Verde population and biogeographical patterns. African Zoology 45(2): 291-298. IF=1.018.


277

288. Vasconcelos, V, Azevedo, J, Silva, M, Ramos, V. 2010. Effects of marine toxins on the reproduction and early stages development of aquatic organisms. Marine Drugs 8(1): 59-79. IF=3.471.

289. Vasconcelos, VM, Vale, M, Martins, A, Antunes, A, Azevedo, J, Welker, M, Lopez, O, Montejano, G. 2010. First report on the occurrence of microcystins in planktonic cyanobacteria from Central Mexico analyzed by molecular, immunological and chemical methods. Toxicon 56: 425-431. IF=2.451.

290. Veiga, P, Besteiro, C, Rubal, M. 2010. Meiofauna communities in exposed sandy beaches on the Galician coast (NW Spain), six months after the Prestige oil spill: the role of polycyclic aromatic hydrocarbons (PAHs). Scientia Marina 74: 385-394. IF=1.079.

291. Veiga, P, Besteiro, C, Rubal, M. 2010. The role of sediment type on the vertical distribution of meiofauna at two Galician rias (NW Iberian Peninsula). Cahiers de Biologie Marine 51: 249-263. IF=0.455.

292. Veiga, P, Ribeiro, J, Gonçalves, JMS, Erzini, K. 2010. Quantifying recreational shore angling catch and harvest in the south of Portugal (Northeast Atlantic): implications for conservation and integrated fisheries management. Journal of Fish Biology 76: 2216-2237. IF=1.330.

293. Vergara-Chen,C, González-Wangüemert, M, Marcos, C, Pérez-Ruzafa, A. 2010. Genetic diversity and connectivity remain high in Holothuria polii (Delle Chiaje 1823) across a coastal lagoon-open sea environmental gradient. Genetica 138: 895-906. IF=2.358.

294. Viegas, CS, Conceição, N, Fazenda, C, Simes, DC, Cancela, ML. 2010. Expression of Gla-rich protein (GRP) in newly developed cartilage-derived cell cultures from sturgeon (Acipenser naccarii). Journal of Applied Ichthyology 26: 214-218. IF=0.945.

295. Vieira, V, Santos, R. 2010. Demographic mechanisms determining the dynamics of the relative abundance of phases in biphasic life cycles. Journal of Phycology 46: 1128-1137. IF=2.239.

296. Villanueva, RD, Sousa, AMM, Goncalves, MP, Nilsson, M, Hilliou, L. 2010. Production and properties of agar from the invasive marine alga, Gracilaria vermiculophylla (Gracilariales, Rhodophyta). Journal of Applied Phycology 22(2): 211-220. IF=1.792.

297. Voelker, AHL, Rodrigues, T, Billups, K, Oppo, D, McManus, J, Stein, R, Hefter, J. Grimalt, JO. 2010. Variations in mid-latitude North Atlantic surface water properties during the mid-Brunhes (MIS 9-14) and their implications for the thermohaline circulation. Climate of the Past 6(4): 531-552. IF=2.821.

298. Wallenstein, FM, Peres, SD Xavier, ED, Neto, AI. 2010. Phytobenthic communities of intertidal rock pools in the eastern islands of Azores and their relation to position on shore and pool morphology. Arquipélago. Life and Marine Science 27: 9-20.

299. Wenning, RJ, Nunns Jr, WR, Stahl Jr, RG, Guilhermino, L, Menzie, C, Römbke, J, Helm, RC. 2010. Global climate change and environmental contaminants: a SETAC call for research. Integrated Environmental Assessment and Management 6(2): 197-198.

300. Wernberg, T, Thomsen, MS, Tuya, F, Kendrick, GA, Staehr, PA, Toohey, BD. 2010. Decreasing resilience of kelp beds along a latitudinal temperature gradient: potential implications for a warmer future. Ecology Letters 13: 685-694. IF=15.253.


278

301. Wernberg, T, Tuya, F, Thomsen, MS, Kendrick, GA. 2010. Turban snails as habitat for foliose algae: contrasting geographical patterns in species richness. Marine and Freshwater Research 61: 1237-1242. IF=1.572.

302. Xavier JC, Cherel Y, Assis CA, Sendão J, Borges, TC. 2010. The feeding ecology of Conger eels (Conger conger) in the North-east Atlantic. Journal of the Marine Biological Association of the United Kingdom 90: 493-501. IF=0.933.

PUBLICATIONS IN INTERNATIONAL PEER REVIEWED PROCEEDINGS

OTHER INTERNATIONAL PUBLICATIONS

1. Anras, L, Boglione, C, Cataudella, S, Dinis, MT, Livi, S, Makridis, P, Marino, G, Ramalho, A, Yúfera, M. 2010. The current status of extensive and semi-intensive aquaculture practices in Southern Europe. Aquaculture Europe 35(2): 12-17.

2. Arenas, F. 2010. Macroalgal marine invasions: Some highlights and knowledge gaps. Algas – Boletin de la Sociedad Española de Ficología 43: 16-19.

3. Balasubramanian, A, Meenakumari, B, Boopendranath, MR, Pravin, P, Erzini, K. 2010. Mesh selectivity of drift gillnet for Caranx sexfasciatus and Caranx tille. Fishery Technology 47: 111-120.

4. Bǿrrensen, T, Boyen, C, Dobson, A, Höfle, M, Ianora, A, Jaspars, M, Kijjoa, A, Olafsen, J, Querellou, J, Rigos, G, Wijffels, R. 2010. Marine Biotechnology: A new vision and strategy for Europe. Marine Board-ESF Position Paper 15: 1-91.

5. Cunha, ME, Quental-Fereira, H, Boglione, C, Palamara, E, Gavaia, P, Pousão-Fereira, P. 2010. Rearing fish larvae for extensive and semi-intensive aquaculture - the “natural” mesocosms system. Aquaculture Europe 35(2): 23-26.

6. Delongueville, C, Scaillet, R, Afonso CML. 2010. Première signalisation de Nassarius sesarmus (Marrat, 1877) le long des côtes européennes de l’Atlantique Nord-Est (Algarve - sud du Portugal). NOVAPEX / Société Belge de Malacologie 11(4): 117-119.

7. Dias, J, Conceição, LEC, Rodrigues, V, Colen, R, Ramalho Ribeiro, A, Ferreira, H, Aires, T, Silva, JMG, Escórcio, C, Valente, LMP. 2010. Aquaculture growth has triggered environmental concerns. Aquaculture Europe 35(2): 5-11.

8. Guerreiro, PM, Bataille, AM, Renfro, JL. 2010. PiT-like transporters are associated with inorganic phosphate transport by choroid plexus of spiny dogfish shark (Squalus acanthias). Bulletin MDIBL 49: p.5.

9. Quental-Ferreira, H, Ramalho Ribeiro, A, Dias, J, Yúfera, M, Arias, AM, Falcão, M, Serpa, D, Aires, T, Pousão-Ferreira, P, Cunha, ME, Valente, LMP, Dinis, MT, Conceição, L. 2010. Sustainable semi-intensive polyculture of seabream and sole in earthern ponds. Aquaculture Europe 35(3): 17-21.

10. Ramalho, A, Dinis, MT. 2010. Portuguese aquaculture: current status and perspectives. Aquaculture Europe 35(3): 5-12.


279

11. Silva, A, Brotas, V, Orive, E, Neto, AI. 2010. First records of Ostreopsis heptagona, Ostreopsis cf. siamensis and Ostreopsis cf. ovata in Atlantic waters of the Azores Archipelago (Portugal). Harmful Algal News 42: 1-2.

12. Silva, TS, Cordeiro, O, Jessen, F, Dias, J, Rodrigues, PM. 2010. On the reproducibility of a fractionation procedure for fish muscle proteomics. American Biotechnology Laboratory 28(4): 8-13.


14. Vaz-Pires, P, Ramalho, A, Jesus, A, Soares, F, Conceição, L, Dinis, MT, Ribeiro, L, Pousão-Ferreira, P, Cunha, ME, Quental-Ferreira, H, Yúfera, M, Marino, G, Boglione, C, Cataudella, S, Hussenot, J, Begout, M-L, Makridis, P, Buard, E, Blachier, P. 2010. A joint certification system for sustainable non-intensive aquaculture: a proposal from the SEACASE Project. Aquaculture Europe 35(2): 17-22.

OTHER NATIONAL PUBLICATIONS

1. Araújo, NCP, Bringela, A, Veríssimo, E, O’Neill, PM, Cristiano, MLS. 2010. New peptidic cysteine protease inhibitors, Revista Portuguesa de Farmácia, vol. LII (4): 32.

2. Araújo, NCP, Cabral, LIL, Pensée, L, Sabbani, O’Neill, PM, Cristiano, MLS. 2010. Design and synthesis of endoperoxide hybrids directed to plasmodial DNA, Revista Portuguesa de Farmácia, vol. LII (4): 116.

3. Cabral, JP. 2010. Gonçalo Sampaio e a história da botânica. Memórias da Sociedade Broteriana, volume XXXIV, Sociedade Broteriana, Coimbra, Portugal.

4. Chícharo, L, Chícharo, MA, Ben-Hamadou, R. 2010. Eco-hidrologia em estuários e zonas costeiras: o caso do Estuário de Guadiana. In: A Gestão da água e dos Recursos Hídricos. Revista Internacional em Língua Portuguesa, Vol III, nº22: 55-66.

5. Gonçalves, J, 2010. Conversões de sistemas de coordenadas nacionais para ETRS89 utilizando grelhas. Actas da 6ª Conferência Nacional de Cartografia e Geodesia, 7-8 Maio 2009, Edições Lidel, 9 p.

6. Pais-Barbosa, J, Veloso-Gomes, F, Taveira Pinto, F. 2010. Análise de formas e padrões costeiros como indicadores de erosão na zona costeira noroeste de Portugal. Revista da Associação Portuguesa de Recursos Hídricos 31(1): 15-26.

7. Petan, S, Taveira-Pinto, F, Mikoš, M, Pais-Barbosa, J. 2010. Modelação da erosão do solo da bacia hidrográfica do Rio Leça, com a Equação RUSLE e SIG. Revista da Associação Portuguesa de Recursos Hídricos 31(1): 99-110.

8. Rangel, MO, Dentinho, TP, Araújo, G, Lopes, J, Gonçalves, JMS, Erzini, K. 2010 Análise custo viagem de roteiros subaquáticos (de apneia) na Praia da Marinha (Algarve). Revista Portuguesa de Estudos Regionais 22: 77-89.


280

B. ADVANCED TRAINING

PhD THESES

1. Azevedo, Alberto Carlos de Oliveira Antunes. 2010. Sistema integrado de modelação para apoio e mitigação de acidentes de hidrocarbonetos em estuários e orla costeira. PhD thesis, LNEC/University of Lisbon. Supervisors: Anabela Pacheco de Oliveira, José CPB Teixeira da Silva.

2. Barrento, Sara. 2010. Nutritional quality and physiological responses to transport and storage of live crustaceans traded in Portugal. PhD thesis in Animal Science, ICBAS, University of Porto. Supervisor: Maria Leonor Nunes (IPIMAR), Co-supervisor: Paulo Vaz-Pires.

3. Bartilotti, Cátia Alexandra Vieira. 2010. Decapod crustacean larvae dynamics in the west continental coast of Portugal: the region adjacent to Aveiro coastal lagoon as model. PhD thesis in Marine Ecology, University of Algarve. Supervisor: Antonina dos Santos (IPIMAR), Co-supervisor: Margarida Castro.

4. Couto, Maria Nazaré PFS. 2010. Investigation of physical, chemical and biological factors implicated in the biological remediation of nonvolatile petroleum hydrocarbons. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto.

5. Faria, Ana. 2010. Ontogeny of behavioural abilities in temperate reef fish larvae. PhD thesis, University of Algarve. Supervisors: Emanuel Gonçalves (ISPA), Maria Alexandra Chícharo.

6. Francisco, Claire Juliana. 2010. Parasitas do mexilhão Mytilus galloprovincialis Lamark, 1819 (BIVALVIA: MYTILIDAE) com relevância para Trematoda: ciclo de vida, características morfológicas e moleculares. PhD thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Maria João Santos.

7. Gonçalves, Hernâni. 2010. Automatic registration of satellite images. PhD thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: José A Gonçalves, Co-supervisor: Luís Corte-Real (INESC Porto, Faculty of Engineering, University of Porto).

8. Guerreiro, Liliana Isabel Tomé dos Anjos. 2010. Biochemical and biological characteristics of novel piscine forms of parathyroid hormone and parathyroid hormone related peptide: a proteomic approach. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power.

9. Leão, Pedro. 2010. Allelopathic properties of cyanobacteria isolated from Portuguese freshwaters. PhD thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.

10. León Cisneros, Karla. 2010. Filogeografia do género Scinaia (Rhodophyta) de áreas transicionais. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto, Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).

11. Lima, Inês de Marrazes. 2010. Ecotoxicological effects of petrochemical products on natural populations of Mytilus galloprovincialis inhabiting rocky shores along the NW coast of Portugal.


281

PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Lúcia Guilhermino, Co-supervisor: Amadeu MVM Soares (CESAM, University of Aveiro).

12. Marçalo, Ana Luisa Barreto 2010. Avaliação de stress em sardinha (Sardina pilchardus) durante a pesca do cerco Universidade do Algarve Supervisors: Karim Erzini, Yorgos Stratoudakis (IPIMAR).

13. Martins, Florbela Alexandra Silva Vieira. 2010. Evolution of the extracellular matrix in deuterostomes and the influence of calcitropic hormones. PhD thesis in Molecular Biology, University of the Algarve. Supervisor: Deborah M Power.

14. Neves, Mónica. 2010. Bio-removal of toxic metals by metal resistant anaerobic bacteria: molecular characterization and performance studies. PhD thesis in Environmental Biotechnology, University of Algarve. Supervisor: Maria Clara Costa, Co-supervisor: Raul Barros.

15. Pereira, A. 2010. Desenvolvimento de um sistema integrado de modelação, calibração e gestão de ecossistemas costeiros, com recurso a agentes inteligentes. PhD in Electronic Engineering and Computers, Faculty of Engineering, University of Porto. Supervisor: Pedro Duarte.

16. Ribeiro, Ana Rita de Araújo. 2010. Thyroid status in senegalese sole (Solea senegalensis) larvae – effects of iodine and selenium supplementation. PhD thesis in Aquaculture, University of Algarve. Supervisors: Maria Teresa Dinis, Laura Ribeiro (IPIMAR), Mari Moren (NIFES, Norway).

17. Ribeiro, Cláudia Maria Rosa. 2010. Chemical identification and monitoring of natural and xenoestrogens compounds in the estuaries of the estuaries of the Portuguese rivers Douro, Mondego and Sado – Insights on their disruption potential by in vitro exposures of testis from the model organism Nile Tilapia (Oreochromis niloticus). PhD Thesis in Biomedical Sciences, ICBAS, University of Porto. Supervisor: Maria João Rocha, Co-supervisors: Eduardo Rocha, Maria Elisabeth Tiritan.

18. Rosas Alquicira, Edgar Francisco. 2010. Filogeografia de algas Corallinales (Rhodophyta) das Califórnias. PhD thesis in Marine Botany, University of the Azores. Supervisor: Ana I Neto, Co-supervisor: Rafael Riosmena Rodríguez (University Baja California Sur, México).

19. Santos, Paulo Jorge Rosa. 2010. Análise da Interacção de Navios com Dispositivos de Acostagem e Amarração. Estudo em Modelo Físico do Posto “A” do Terminal de Petroleiros do Porto de Leixões. PhD thesis in Civil Engineering, Faculty of Engineering, University of Porto. Supervisor: Fernando Veloso Gomes, Co-supervisor: Francisco Taveira Pinto.

20. Silva, Amélia Cláudia Figueiredo. 2010. Nutritional regulation of lipid deposition in blackspot seabream (Pagellus bogaraveo). PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa M.P. Valente, Co-supervisoror: Geneviéve Corraze (INRA, France).

21. Silva, Joana Margarida Guimarães. 2010. Use of alternative protein sources in diets for senegalese sole (Senegalese sole Kaup, 1858) juveniles. PhD thesis in Aquatic Sciences, ICBAS, University of Porto. Supervisor: Luisa M.P. Valente, Co-supervisoros: Marite Espe (NIFES, Norway), Luís Conceição.

22. Silva, Raquel Castro Alves Ferreira. 2010. Avaliação Experimental e Numérica de Parâmetros Associados a Modelos de Evolução da Linha de Costa. PhD thesis in Civil Engineering, Faculty of Engineering, University of Porto. Supervisor: Fernando Veloso Gomes, Co-supervisor: Francisco Taveira Pinto.


282

23. Sousa, Joaquim, 2010. Integration of DInSAR and DGPS for location and characterization of active faults. Supervisor: Luísa Bastos, Co-supervisor: Ramon Hanssen (Delft University of Technology, The Netherlands).

24. Viegas, Carla. 2010. Identification and characterization of a new calcium binding protein from sturgeon, Gla rich protein or GRP. PhD thesis in Biochemistry – Cellular and Molecular Biology, University of Algarve. Supervisor: M. Leonor Cancela, Co-supervisor: Dina C Simes

MASTER THESES

1. Afonso, João Marcos da Silva. 2010. Padrões verticais e inter-anuais nas assembleias de larvas de peixe junto ao substrato rochoso do Parque Marinho da Arrábida. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).

2. Almeida, Helena Lopes. 2010. Avaliação da Qualidade da Biológica da água de um ecossistema fluvial - Ribeira da Madalena. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

3. Álvaro, Nuno Miguel Vaz. 2010. Proposta de plano de monitorização para a área protegida para a gestão de habitats ou espécies do Ilhéu de Vila Franca do Campo. Master thesis in Coastal Management, University of the Azores. Supervisor: Fátima Alves (University of Aveiro), Co-supervisor: Ana I Neto.

4. Amorim, Eva. 2010. Bacterial indicators of bathing water quality in Douro estuary and Porto coastal front. Master thesis in Sciences of the Sea-Sea Resources, ICBAS, University of Porto. Supervisor: Adriano A Bordalo e Sá, Co-supervisor: Isabel Azevedo.

5. Andrade, André Alçada Baptista Rebelo. 2010. Identification of reliable reference gene(s) for quantitative-PCR studies in male and female tissues and during oogenesis in the Mozambique tilapia, Oreochromis mossambicus. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Adelino Canario, Co-supervisor: Laurence Deloffre.

6. Araújo-Silva, Catarina de Lourdes. 2010. Taxonomia e aspectos ecologicos de Tanaidacea (crustacea: Peracarida) da plataforma continental e bancos oceanicos no Programa REVIZEE. Master thesis, University of Pernambuco, Recife, Brazil. Supervisor: Kim Larsen.

7. Baptista, Miguel. 2010. Determination of age and growth of Galeus melastomus, Rafinesque, 1810, a deep water shark, using a modified cobalt nitrate technique European Master Thesis in Marine Biodiversity and Conservation, University of Algarve and University of Ghent. Supervisors: Rui Coelho, Karim Erzini.

8. Barbosa, Ana. 2010. Estudo de parâmetros hematológicos de pregado (Psetta maxima Linnaeus, 1758) de cultura Master thesis in Biological Aquatic Resources, Faculty of Sciences, University of Porto. Supervisor: Cristina Cruz.

9. Barradas, Ana Rita. 2010. Laminaria ochroleuca canopy effect on intertidal under storey benthic assemblages in northern Portugal. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Ester Serrao, Filipe Alberto.


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10. Berkovic, Buga. 2010. Effect of habitat fragmentation on bivalve larvae recruitment: study of Pinna nobilis and Pectinidae species larvae. Master thesis in Marine Biodiversity and Conservation, University of Algarve. Co-supervisors: Ester Serrao, Iris Hendriks (IMEDEA, CSIC).

11. Capela, Ricardo. 2010. Fish otoliths as potential chronological biomarkers of environmental aquatic contamination by heavy metals. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: Alberto Correia, Co-supervisor: Bruno Nunes (University Fernando Pessoa, CESAM).

12. Cardoso, Inês. 2010. Substituição de farinha de peixe por farinhas vegetais em sargo legítimo, Diplodus sargus: efeitos na performance, digestibilidade e alterações da histologia do intestino. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: Aires Oliva Teles, Co-supervisor: Helena Peres.

13. Carrasquinho, Ricardo. 2010. Gestão e maneio de uma unidade de piscicultura em mar aberto na costa sul de Portugal. Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Pedro Pousão-Ferreira.

14. Carvalho, André. 2010. Macrobenthos Biodiversity in Cávado estuary. Master thesis, University of Porto. Supervisor: Paulo Santos

15. Cavaco, Sofia. 2010. Isolamento e caracterização de proteínas contendo Gla da cartilagem e pele de mamífero. Master thesis in Molecular Biology and Microbiology, University of Algarve. Supervisor: Dina Costa Simes.

16. Czepiel, Aneta 2010. Synthesis of saccharyl tetrazoles and investigation of their potential as nitrogen ligands. Master thesis in Chemistry, Faculty of Science and Technology, University of Algarve. Supervisor: Maria de Lurdes Cristiano.

17. Domingues-Santos, Bernardo. 2010. Bioremediation efficiency of Mastocarpus stellatus (Stackhouse) Guiry, in an Integrated Multi-Trophic Aquaculture System. Master in Aquaculture, Interuniversity UB-UAB-UPC. Supervisor: Isabel Sousa Pinto, Co-supervisor: Lourdes Reig (UPC).

18. Duarte, Daniel João da Silva Tavares. 2010. Master thesis in Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: M Soto (University of the Basque Country), Co-supervisor: Lúcia Guilhermino.

19. Elias, Carlos Alexandre. 2010. Produção e expressão de péptidos recombinantes contra dsRNA. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Fernando Cardoso (INETI/UTPAM), Co-supervisor: Deborah Power.

20. Encarnação, Sandra Paula. 2010. Caracterização dos mecanismos de transporte epitelial no intestino da Dourada, Sparus auratus. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Juan Fuentes, Co-supervisor: Deborah Power.

21. Faria, Rosa. 2010. Optimização do processamento de dados GNSS de alta taxa de amostragem em tempo quase-real em modo singular Master thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: Rui Fernandes, Co-supervisor: José A Gonçalves.

22. Fialho, Ana Isa Marques. 2010. Caracterização do comportamento reprodutivo do cavalo marinho de focinho comprido (Hippocampus guttulatus). Master thesis in Marine Biology, University of Algarve.


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23. Filipe, Alexandra. 2010. Clonagem e análise de sequências codificantes de terpeno sintases das plantas aromáticas Thymus mastichina e Thymus albicans. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Natália Marques, Co-supervisor: João Cardoso.

24. Gonçalves, Annie. 2010. Ecotoxicological and histological assessment of the effects of the benzalconium chloride in the mosquito fish (Gambusia holbrooki) after an acute exposition. Master in Pharmaceutical Sciences, Health Sciences Faculty, University Fernando Pessoa. Supervisor: Bruno Nunes (University Fernado Pessoa, CESAM), Co-supervisor: Alberto Correia.

25. Graça, Mariana João Dias. 2010. Caracterização da pesca grossa na ilha de Madeira. Master thesis in Marine Biology, University of Algarve. Supervisors: Karim Erzini, Mafalda Freitas (Marine Biological Station of Funchal).

26. Guedes, Paula ARA. 2010. Avaliação de estratégias de biorremediação para áreas afectadas por derrames de petróleo. Master thesis in Chemistry, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto, Co-supervisor: C Marisa Almeida.

27. Guerra, Luís Miguel. 2010. Análise multivariada da evolução sucessão dunar na Península do Ancão e Ilha da Barreta. Master thesis, University of Algarve. Supervisors: Rui Santos, Vasco Vieira.

28. Guerreiro, Bruno Emanuel 2010. Endoperóxidos com actividade antimalárica: estrutura, reactividade e actividade. Master thesis in Pharmaceutical Sciences, Faculty of Science and Technology, University of Algarve. Supervisor: Maria de Lurdes Cristiano.

29. Jerónimo, Daniel Baptista. 2010. Pesquisa de agentes patogénicos em rodovalho (Psetta maxima L.) de cultura. Master thesis in Aquatic Biologic Resources, Faculty of Sciences, University of Porto. Supervisor: Aurélia Saraiva.

30. Joussef, Samira. 2010. Evolutionary genetics of the AMT domain of the microcystin gene cluster in various cyanobacteria genera. Master thesis in Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: Agostinho Antunes, Co-supervisor: Vítor Vasconcelos.

31. Lima dos Reis, Jandir João. 2010. The genetic mating system in the black striped pipefish, Syngnathus abaster (Risso, 1827) in Ria Formosa, revealed by microsatellites. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Onno Diekmann, Ester Serrao, Filipe Alberto.

32. Lino, Filipe Correia. 2010. Oxygen consumption experiments in the Pepper, furrow shell - Scrobicularia plana (da Costa) under starvation conditions - DEB model parameter estimation. Master thesis in Sciences of Engineering / Zootechnical Engineering, University of Tas-os-Montes and Alto Douro.

33. Ma, Simão. 2010. Efeitos da adição de antioxidantes nos meios de criopreservação de sémen de peixes Teleósteos. Master thesis in Zootechnical Engineering - Animal Production, Faculty of Veterinary Medicine, Technical University of Lisbon. Supervisor: Maria Teresa Dinis, Co-Supervisor: Fernando Ribeiro Alves Afonso.

34. Marinho, Gonçalo. 2010. Efeito do regime de alimentação – diurno, nocturno e misto – no crescimento, na utilização do alimento e na excreção azotada em juvenis de linguado Solea


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senegalensis. Master thesis in Aquatic Biological Resources, Faculty of Sciences, University of Porto. Supervisor: António Paulo Carvalho; co-supervisor: Helena Peres.

35. Marques, Joana Filipa. 2010. Caracterização bioquímica e molecular de estirpes de Yersinia ruckeri isoladas em Portugal. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: José Américo Sousa.

36. Marujo, Débora. 2010. Efeito de um concentrado de microlagas liofilizadas na larvicultura da dourada, Sparus aurata. Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Pedro Pousão-Ferreira.

37. Mateus, Carmen Sofia Cardoso. 2010. Análise do efeito da halófita Salicornia ramosissima na remoção de nutrientes em substrato sólido e em meio hidropónico. Master thesis, University of Algarve. Supervisors: Luís Chícharo, Rui Santos.

38. Mendes, Cristiane. 2010. Caracterização de isolados de Vibrio anguillarum originários de pisciculturas portuguesas. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: José Américo Sousa.

39. Mendes, Ricardo. 2010. Orientação e calibração de dados ALS. Master thesis in Surveying Engineering, Faculty of Sciences, University of Porto. Supervisor: Ismael Colomina, Co-supervisor: José A Gonçalves.

40. Mesquita, João Rodrigo GG. 2010. Norovirus contamination of shellfish from high sanitary production areas of Portugal. Master thesis in Sciences of Engineering / Zootechnical Engineering, University of Tas-os-Montes and Alto Douro.

41. Mesquita, Sofia. 2010. Testing the potential of chitobiase activity of Carcinus maenas as a biomarker in environmental studies. Master thesis in Contamination and Environmental Toxicology, Faculty of Sciences and ICBAS, University of Porto. Supervisor: Laura Guimarães, Co-supervisor: Lúcia Guilhermino.

42. Nascimento, Ângela. 2010. Campanha MPDO para sardinha (IPIMAR, 2005). Caracterização hidrográfica sobre a plataforma continental Ibérica. Aplicação dos dados de perfis de temperatura à datação de ovos de sardinha, efeitos sobre a estimativa de produção de ovos. Master thesis in Oceanography, Faculty of Sciences and Technology, University of Algarve. Supervisor: Paulo Relvas, Co-supervisor: Maria Manuel Angélico (INRB-IPIMAR).

43. Nhanca, Florentino João Lopes. 2010. Caracterização da pesca industrial na ZEE da Guiné-Bissau com vista ao seu desenvolvimento sustentável. Master thesis in Management and Nature Conservation, University of Algarve. Supervisors: Karim Erzini, Jorge MS Gonçalves.

44. Oliveira, Cristiana, 2010. Effects of pesticides in biomarkers and behaviour of Palaemon serratus. Master thesis in Applied Biology: branch of Toxicology and Ecotoxicology, Department of Biology, University of Aveiro. Supervisor: Carlos Gravato.

45. Peixoto, Lúcia. 2010. Avaliação da inclusão de EVOCOL em dietas formuladas para juvenis de linguado (Solea senegalensis Kaup, 1858). Master thesis in Marine Biology - Fisheries and Aquaculture, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Maria Teresa Dinis, Co-supervisor: Mariano Lastra.


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46. Pereira, Ana Margarida. 2010. Efeito de complexos de vanádio na bomba de cálcio de retículo sarcoplasmático. Master thesis in Biotechnology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Manuel Aureliano.

47. Pereira, Fernanda Cristina Rodrigues Malhão. 2010. Characterization of the trout liver cell line RTL-W1 and its potential for carcinogenesis-related studies. Master thesis in Oncology. ICBAS, University of Porto, Supervisor: Eduardo Rocha, Co-supervisor: Rogério AF Monteiro.

48. Pereira, Nuno Miguel Campos Mendes Sousa. 2010. Avaliação do potencial aquícola da população de amêijoas Venerupis aurea (Gmelin, 1791) da ilha de S. Jorge (Açores). Master thesis in Sea Sciences – Marine Resources, ICBAS, University of Porto. Supervisor: Ana I Neto, Co-supervisor: Domitília Matias (IPIMAR).

49. Pimenta, Pedro. 2010. Biodiversity in Serra da Aboboreira, NO Portugal. Master thesis, University of Porto. Supervisor: Paulo Santos.

50. Pinto, Dorabela SSP. 2010. Avaliação da eficiência de remediação biológica (em termos microbiológicos e fitorremediação) de hidrocarbonetos de petróleo num solo contaminado. Master thesis in Environmental Sciences and Technology, Faculty of Sciences, University of Porto. Supervisor: Maria Clara P Basto.

51. Pires, Rita. 2010. Padrões temporais e verticais de pequena escala na ocorrência de larvas de peixe na Ria de Aveiro, Portugal. Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).

52. Purroy Albet, Ariadna. 2010. Spatial assessment and impact of artisanal fisheries´activity in Cap de Creus. Master thesis in Marine Biodiversity and Conservation, University of Algarve. Co-supervisors: Ester Serrao, Rafael Sardá and Susana Requena (CSIC, Barcelona).

53. Reisinho, Inês Isabel Ribeiro Oliveira. 2010. Pesquisa de parasitas de tainha do estuário do rio Douro. Master thesis in Sciences of the Sea - Sea Resources, ICBAS, University of Porto. Supervisor: Aurélia Saraiva.

54. Rocha, Patrícia Carolina Rendall. 2010. Descrição do desenvolvimento larvar de Tripterygion delaisi (Pisces: Tripterygiidae). Master thesis in Marine Biology, University of Algarve. Co-supervisors: Rita Borges, Ester Serrao, Emanuel Gonçalves (ISPA).

55. Rodrigues, Carolina. 2010. Avaliação da Qualidade de Ecológica do Rio Ferreira nos Concelhos de Valongo e Paços de Ferreira. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

56. Roldão, Mariana. 2010. Ecotoxicologia e Educação. Dos estudos no estuário do Rio Ave à elaboração de ferramentas educativas. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.

57. Salgado, Lídia. 2010. Desinfecção de águas naturais contaminadas com cianobactérias por fotocatálise solar. Master thesis in Environmental Engineering, Faculty of Engineering, University of Porto. Cu-supervisor: Vítor Vasconcelos, Rui Boaventura and Vítor Vilar (Faculty of Engineering, University of Porto).

58. Santos, Célia. 2010. Reconstrução das condições paleoambientais e paleoclimáticas no estuário do Rio Tejo durante o Holocénico (~12 000 anos). Master thesis in Sea Sciences – Marine Resources, ICBAS, University of Porto. Supervisor: F Abrantes.


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59. Santos, Marta Susana Amaro. 2010. An histopathological and stereological study of proliferative hepatocellular lesions in a fish model of experimental carcinogenesis and in a sentinel fish. Master thesis in Oncology. ICBAS, University of Porto, Supervisor: Eduardo Rocha, Co-supervisor: Rogério A.F. Monteiro.

60. Silva, Ana. 2010. Contribuição para o estudo da parasitose provocada por Kudoa sp. em Sardina pilchardus (Walb., 1792.) Master thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Cristina Cruz.

61. Silva, Diogo. 2010. Discrimination of Trisopterus luscus (Linnaeus 1758) stocks in the northern of Portugal using otolith elemental fingerprints. Master thesis in Sea Sciences - Marine Resources, ICBAS, University of Porto. Supervisor: Alberto Correia, Co-supervisor: Paulo Santos.

62. Silva, Maria João. 2010. Control of ammonia during fish transport: the usage of AmQuel® in laboratory trials. Master thesis in Marine Biology, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Teresa Modesto, Co-supervisor: Pedro Guerreiro.

63. Silva, Marisa. 2010. Tetrodotoxin ocurrence in Portuguese coastal waters: first report on Atlantic coastal waters. Master thesis in Biology, Faculty of Sciences, University of Porto. Supervisor: Vítor Vasconcelos.

64. Silva, Rita Ribeiro. 2010. Hepatic regeneration and the role of platelets. Master thesis, Medicine, ICBAS, University of Porto, Supervisor: Eduardo Rocha.

65. Simão, Márcio. 2010. Identification and characterization of two different transcripts of ferritin in the clam R. Decussatus. Expression of the corresponding isoforms to evaluate some of their biological properties. Master thesis in Molecular and Microbial Biology, Faculty of Sciences and Technology, University of Algarve. Supervisor: Leonor Cancela, Co-supervisor: Ricardo Leite.

66. Sobral, João Miguel Banha. 2010. Influência do gene HMOX1 (Heme Oxigenase I) na genética dos acidentes vasculares cerebrais. Master thesis in Biological Engineering, Faculty of Sciences and Technologies, University of Algarve. Supervisor: Astrid Vicente (INS Dr Ricardo Jorge), Co-supervisor: Deborah Power.

67. Sousa, Raquel Cristina Ferreira. 2010. Avaliação da Qualidade da água da Ribeira da Madalena. Influência da vegetação riparia nos grupos funcionais de macroinvertebrados bentónicos. Master thesis in Biology and Management of Water Quality, Faculty of Sciences, University of Porto. Supervisor: Natividade Vieira.

68. Vasco, Enoque Canganjo. 2010. Caracterização hidrográfica e química da região costeira de Benguela, Angola. Master thesis in Oceanography, Faculty of Sciences and Technology, University of Algarve. Supervisor: Paulo Relvas, Co-supervisor: Alexandra Cravo.

69. Viegas, Sofia. 2010. Development of a more objective index for the quantification of environmental impacts. Master thesis, University of Porto. Supervisor: Paulo Santos, Co-supervisor: Sílvia Mesquita.

70. Voguel, Camille. 2010. Geostatistical exploration of integrated VMS data from the Aran Ground Nephrops fishery with associated survey estimates: Establishing the relationship between fishing, biotic and abiotic variables. Erasmus Mundus Master in Biodiversity and Conservation, University of Algarve. Supervisor: Colm Lordan (Marine Institute, Galaway, Ireland), Co-supervisor: Margarida Castro.


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C. COMMUNICATIONS

COMMUNICATIONS IN INTERNATIONAL SCIENTIFIC MEETINGS

1. Abrantes, F. 2010. Past climate fluctuations: the Iberian Margin Record. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal. (Oral communication)

2. Abreu, MH, Pereira, R, Buschmann, A, Yarish, C, Sousa-Pinto, I. 2010. Land-based Integrated Multi-Trophic Aquaculture: the red seaweed Gracilaria vermiculophylla (Ohmi) Papenfuss as the biofilter component. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

3. Abreu, MH, Pereira, R, Figueroa, F, Buschmann, A, Yarish, C, Sousa-Pinto, I. 2010. Integrated multi-trophic aquaculture in Portugal: Experiments with the red seaweed Gracilaria vermiculophylla (Ohmi) Papenfuss in a sole and turbot fish farm. World Aquaculture 2010, 1-5 March, San Diego, USA. (Oral communication)

4. Abreu, MH, Pereira, R, Korbee, N, Figueroa, F, Buschmann, A, Yarish, C, Sousa-Pinto, I. 2010. Sustainable exploration of a non-indigenous species: the case study of Gracilaria vermiculophylla (Ohmi) Papenfuss in Portugal. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. (Oral communication)

5. Agüero-Chapin, G, Pérez-Machado, G, Molina-Ruiz, R, Pérez-Castillo, Y, Morales-Helguera, A, Vasconcelos, V, Antunes, A. 2010. A QSFR-like model to detect proteinaceous bacteriocins. Its practical application to the discovery of new bacteriocin-like domains. IV International Symposium on Chemistry, 1-4 June, Santa Clara, Cuba.

6. Alex, A, Vasconcelos, V, Tamagnini, P, Santos, A, Antunes, A. 2010. Cyanobacteria- sponge symbioses: an evolutionary genomic approach. VIII World Sponge Conference, 20-24 September, Girona, Spain.

7. Alexandrino, M, Canário, AV, Costa, MC. 2010. Characterization of a high performance bacterial consortium for AMD bioremediation. 14th International Biotechnology Symposium, 14-18 September, Rimini, Italy.

8. Alfonso, A, Rodriguez, P, Otero, P, Vasconcelos, V, Vale,

P, Katikou,

P, Molgo,

J, Botana,

LM. 2010.

The changing profile of marine toxins in Europe. 18th Meeting in Advanced Toxicology, 13-14 December, Paris, France. (Oral communication)

9. Almeida, CMR, Vasconcelos, MTSD. 2010. Evaluation ex-situ of the potential of Halimione portulacoides for metal phytoremediation. International Phytotechnologies Conference 2010, 26 - 29 September, Parma, Italy.

10. Almeida, JR, Gravato, C, Guilhermino, L. 2010. Bioassays based on fish swimming behaviour for the marine environment: short-term effects of pyrene on the sea bass. ICES Annual Science Conference, 20-24 September, Nantes, France.

11. Almeida, JR, Gravato, C, Guilhermino, L. 2010. Integrating ecologically relevant parameters in environmental studies: linking swimming performance with biomarkers responses in the sea bass (Dicentrarchus labrax). VIII Iberian Congress and V Iberoamerican Congress of Environmental


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Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

12. Almeida, JR, Gravato, C, Guilhermino, L. 2010. Linking sub-individual responses with behaviour: a study with sea bass (Dicentrarchus labrax) exposed to pyrene. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

13. Almeida, JR, Luis, L, Vieira, LR, Oliveira, M, Gravato, C, Guilhermino, L. 2010. Acute effects of pyrene on four marine species with different ecological functions. SETAC North America 31st Annual Meeting, 7-11 November, Portland, USA.

14. Álvaro, NV, Prestes, ACL, Neto, AI. 2010. Sea bed mapping and subtidal communities identification in Azorean MPAs – Preliminary results providing tools for marine resources conservation. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal.

15. Alves Martins, D, Morais, S, Castanheira, MF, Martínez-Rodríguez, G, Mendes, AC, Pousão-Ferreira, P, Rocha, F, Yúfera, M, Bandarra, NM, Conceição, LEC. 2010. Effects of dietary arachidonic acido n cortisol production and the expression of genes related to stress response, lipid and eicosanoid metabolism in Senegalese sole post-larvae. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

16. Alves Martins, D, Morais, S, Rocha, F, Castanheira, MF, Martínez-Rodríguez, G, Yúfera, M, Bandarra, NM, Bell, JG, Conceição, LEC. 2010. Dietary fatty acids affect the expression of genes related to stress response, lipid and eicosanoid metabolism in gilthead seabream (Sparus aurata) larvae. 9th International congress on the Biology of Fish, 5-9 July, Barcelona, Spain. (Oral communication)

17. Amorim, A, Oliveira, E, Lobo-da-Cunha, A. 2010. A novel approach in the study of the male copulatory system of Bulla striata (Cephalaspidea). 3rd International Workshop on Opisthobranchs, 1-4 September, Vigo, Spain.

18. Amorim, E, Ramos, S, Azevedo, I, Bordalo, AA. 2010. Relevance of temporal and spatial variability for monitoring the microbiological water quality in an urban bathing area. The Water Research Conference, 11-14 April, Lisbon, Portugal.

19. Anjos, MC, Medeiros, J, Neto, AI, Barreto, MC. 2010. Polyphenols and antioxidant activity in macroalgae from Azores. International Symposium FloraMac 2010, 23-25 Spetember, Ponta Delgada, Azores, Portugal. Book of abstracts, p. 84.

20. Antunes, A, Philip, S, da Fonseca, RR, Reis-Henriques, MA, Vasconcelos, V, Castro, LFC, Santos, MM. 2010. The modulation of retinoic acid signalling pathways by environmental pollutants in marine metazoans. CIESM 39th Congress, 10-14 May, Venice, Italy. (Oral communication)

21. Antunes, A, Philip, S, da Fonseca, RR, Reis-Henriques, MA, Vasconcelos, V, Castro, LFC, Santos, MM. 2010. Evolution and modulation of retinoic acid signalling pathways in metazoans. VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

22. Antunes, A, Philip, S, da Fonseca, RR, Reis-Henriques, MA, Vasconcelos, V, Castro, LFC, Santos, MM. 2010.The modulation of retinoic acid signaling pathways by environmental pollutants in marine metazoans. CIESM 39th Congress, 10-14 May, Venice, Italy. (Oral communication)


290

23. Antunes, A. 2010. The role of genetics in the conservation of biodiversity. 18th Annual South East Asia Zoo Association - SEAZA Conference, 3-6 November, Bali, Indonesia. (Oral communication)

24. Antunes, A. 2010. The role of genetics in wildlife rescue. 2nd International Conference on Wildlife Rescue in East and Southeast Asia, 9-13 November, Cuc Phuong National Park, Vietnam. (Oral communication)

25. Aragão, C, Pinto, W, Dinis, MT. 2010. Dietary tyrosine utilisation in flatfish: the choice between metamorphosis and stress response. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

26. Araújo, IB, Caldeira, R, Couvelard, X. 2010. Island induced (sub) mesoscale features around Madeira Archipelago (initial findings). 3th International Workshop SeaSAR 2010 - Advances in SAR Oceanography from ENVISAT, ERS and ESA third party missions, 25-29 January, Frascati, Italy.

27. Araújo, J, Mota, M, Braga, C, Sousa, R, Roleira, A, Carvalho, R, Antunes, C. 2010. Exotic macrofauna at the River Minho international. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

28. Araújo-Silva, CDL, Larsen, K, Coelho, PA. 2010. Registro de novas ocorrências e espécies de Tanaidacea (Peracarida) coletados durante o Programma Revizee- NE. 6th Congress of the Brazilian Crustacean Society, 7-10 November, Bahia, Brazil. (Oral communication)

29. Araújo-Silva, CDL, Souza-Filho, JF, Coelho, PA, Larsen, K. 2010. Distribuição e ecologia de Tanaidacea (Peracarida) da plataforma continental e bancos oceanicos de Nordeste do Brazil. 6th Congress of the Brazilian Crustacean Society, 7-10 November, Bahia, Brazil.

30. Arnaud-Haond, S, Teixeira, S, Moalic, Y, Desbruyéres, D. 2010. Networks of marine Biodiversity. Biodiversity in the 21st Century, 10-13 October, Ramat-Hanadiv, Israel. (Oral communication)

31. Arnaud-Haond, S, Teixeira, S. 2010. Overview of connectivity at vents and seeps- temporal and spatial scales. Workshop on the Design of Marine Protected Areas for Hydrothermal-Vent and Cold-Seep Ecosystems Potentially Threatened by Human Activities in the Deep Sea, 31 May-4 June, Dinard, France. (Oral communication)

32. Auamcharoen, W, Chandrapatya, A, Naengchomnong W, Kijjoa, A. 2010. A new tetralone from Diospyros cauliflora. 58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, 29 August-2 September, Berlin, Germany.

33. Azevedo, C, Casal, G, Marques, D, Silva, E, Matos, P, Matos, E. 2010. Dados ultrastruturais de Myxobolus sp. (Phylum Myxozoa), parasita de Brycon hilarii, teleósteo do Pantanal. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil.

34. Azevedo, C, Casal, G, Matos, P, Matos, E. 2010. Estrutura fina do esporo de Triangulamyxa sp. (Myxozoa, Myxosporea), um parasita da bexiga urinária de Psittacus colomesus (Teleostei) do rio Tocantins afluente do rio Amazonas. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil.

35. Azevedo, I, Ramos, S, Mucha, A, Bordalo, A. 2010. Applicability of ecological assessment tools for management decision-making: a case study from the Lima Estuary, Portugal. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)


291

36. Baptista, M, Figueiredo, I, Coelho, R, Erzini, K. 2010. Age and growth of the deep water shark Galeus melastomus (Elasmobranchii: Scyliorhinidae), in the southern coast of Portugal. ICES Annual Science Conference, 20-24 September, Nantes, France.

37. Baptista, MS, Cianca, RCC, Lopes, VR, Almeida, CMR, Vasconcelos, VM. 2010. BMAA quantification in estuarine cyanobacteria by HPLC and CE – a comparative study. Cyano 2010, 8th International Conference on Toxic Cyanobacteria - ICTC8, 29 August-4 September, Istanbul, Turkey.

38. Barbosa, N, Vale, C, Santos, P. 2010. Heavy metals in white clam Spisula solida from the northern Portuguese coast. VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

39. Barbosa, SM, Fernandes, MJ, Lázaro, C, Nunes, AL, Cipollini, P. 2010: Comparison of coastal altimetry and tide gauge data along the west Iberian coast. EGU General Assembly 2010, 2-7 May, Wien, Austria.

40. Barbosa, SM, Fernandes, MJ, Lázaro, C, Nunes, AL, Pires, N, Cipollini, P. 2010. Frequency‐dependent comparison of satellite altimetry and tide gauge measurements. 4th Coastal Altimetry Workshop, 14-15 October, Porto, Portugal.

41. Barbosa, SM, Fernandes, MJ, Lázaro, C, Nunes, AL, Pires, N, Cipollini, P. 2010. Validation of coastal altimetry data along the west Iberian coast. ESA Living Planet Symposium, 28 June-2 July, Bergen, Norway. (Oral communication)

42. Barrios, C, de la Cruz, D, Franco, L, Dias, J, Dinis, MT. 2010. Aquaculture production in Guatemala: its current status and implications in promoting food security. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

43. Barros, P, Martins, R. 2010. Modelos biológicos de investigação em toxicologia de cianobactérias. I International Health Congress Gaia-Porto, 23-25 September, Vila Nova de Gaia, Portugal.

44. Bayona, J, Readman, J, Thomas, K, Beiras, R, Guilhermino, L. 2010. Implementation of risk assessment methodologies for oil and chemical spills in the European marine environment (RAMOCS). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

45. Ben-Hamadou, R. 2010. Ecohydrology & biodiversity in coastal zones. International Workshop on Tools to the Ecohydrologic Management of Coastal Systems in Arid and Semi-arid Zones, 17-21 July, Cairo, Egypt. (Oral communication)

46. Ben-Hamadou, R. 2010. The Ecohydrology paradigm. International Workshop on Tools to the Ecohydrologic Management of Coastal Systems in Arid and Semi-arid Zones, 17-21 July, Cairo, Egypt. (Oral communication)

47. Bensimon-Brito, A, Cancela, ML, Huysseune, A, Witten, PE. 2010. Zebrafish (Danio rerio) caudal complex - a model for vertebral fusion. 9th International Congress of Vertebrate Morphology, 26-31 July, Punta del Este, Uruguay.

48. Bensimon-Brito, A, Cardeira-da-Silva, J, Cancela, ML, Huysseune, A, Witten, PE. 2010. Zebrafish (Danio rerio) vertebral column – two distinct mineralization patterns. 9th International Meeting on Zebrafish Development and Genetics, 16-20 June, Madison, USA.


292

49. Bio, A, Meirinho, A, Ramírez, I, Geraldes, P. 2010. Empirical modelling of Balearic shearwater

(Puffinus mauretanicus) census data for the Portuguese Atlantic. GIslands 2010 International Summer School on Marine Spatial Planning, 7-12 August, Ponta Delgada, Portugal. (Oral communication)

50. Bio, A, Vieira, N, Costa, MJ, Valente, A. 2010. Assessment of habitat and water quality of the Portuguese river Febros and one of its tributaries. XV Congress of the Iberian Association of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. (Oral communication)

51. Bio, AMF, Vieira, MN. 2010. Artisanal salinas – unique wetland habitats worth preserving: the

case of Aveiro, Portugal. SIL 2010 31st Congress of the International Society of Limnology, 15-20 August, Cape Town, South Africa. (Oral communication)

52. Boglione, C, Fontagne, S, Gavaia, PJ, Gisbert, E, Kjorsvik, E, Koumoundouros, G, Moren, M, Moutou, K, Witten, PR. 2010. Determinism and causative factors for morphological anomalies in reared European fishes. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-8 October, Porto, Portugal. (Oral communication)

53. Bordalo, AA, Azevedo, I. 2010. How to manage an estuary? An example from the Portuguese legal criteria. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)

54. Borges, A, Carvalho, M, Santos, P. 2010. Computer application for assistance in comparing growth curves of fish stocks. International Statistical Ecology Conference 2010 – ISEC 2010, 2-5 March, Belfast, United Kingdom. (Oral communication)

55. Borges, P, Medale, F, Dias, J, Valente, LMP. 2010. Influence of different Protein/Lipid ratios on growth performance and intermediary metabolism of Senegalese sole. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-8 October, Porto, Portugal. Book of abstracts, pp. 224-225. (Oral communication)

56. Bos, MS, Araújo, IB, Bastos, L, Fernandes, MJ. 2010. A regional set of tide‐level maps for the West Iberian Coast. 4th Workshop on Coastal Altimetry, 14-15 October, Porto, Portugal. (Oral communication)

57. Bos, MS, Bastos, L, Fernandes, RMS. 2010. Investigating the noise properties of GPS stations in Iberia. 15th General Assembly of WEGENER, 14-17 September, Istanbul, Turkey. (Oral communication)

58. Bos, MS, Fernandes, RMS, Araújo, IB, Bastos, L, Vasconcelos, M. 2010. Error analysis of Cascais absolute sea-level change. 2nd International meeting on Data Analysis and Modelling in Earth Sciences. DAMES'2010, 22-24 September, Lisbon, Portugal. (Oral communication)

59. Bos, MS, Penna, NT, Keshin, M, Clarke, PJ, Baker, TF. 2010. Status of ocean tide loading displacement modelling. International GNSS Service (IGS) 2010 Workshop, 28 June-2 July, Newcastle, United Kingdom. (Oral communication)

60. Brandefelt, J, Kjellström, E, Naeslund, JO, Strandberg, G, Voelker, AHL, Wohlfarth, B. 2010. The importance of equilibration in glacial climate simulations. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-12736. (Oral communication)

61. Burgess, G, Coelho, R, Schofield, P. 2010. Australian lantern sharks of the Etmopterus lucifer complex (Etmopteridae). Sharks International, 6-11 June, Cairns, Australia. (Oral communication)


293

62. Cabral, H, Fonseca, V, Gamito, R, Gonçalves, C, Costa, JL, Erzini, K, Gonçalves, J, Martins, J, Leite, L, Andrade, JP, Ramos, S, Bordalo, A, Amorim, E, Neto, JM, Marques, JC, Rebelo, JE, Silva, C, Castro, N, Almeida, PR, Domingos, I, Gordo, LS, Costa, MJ. 2010. Estuarine Fish Assessment Index (EFAI): a multimetric tool for the assessment of the ecological status of transitional waters. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)

63. Caldeira, C, Correia, AT, Gonçalves, F, Nunes, B. 2010. Assessment of metallic pollution using a biomarker approach. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

64. Campinho, MA, Cardoso, JC, Silva, N, Power, DM. 2010. Remodeling the gastrointestinal tract during metamorphosis of the atlantic halibut (Hippoglossus hippoglossus). EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. (Oral communication)

65. Campos, A, Puerto, M, Prieto, A, Cameán, A, Almeida, AM, Coelho, AV, Vasconcelos, V. 2010. Two-dimensional gel electrophoresis of proteins in the mediterranean mussel Mytilus galloprovincialis. Proteomlux 2010 - International Conference on Proteomics in Plants, Microorganisms and Environment, 18-20 October, Luxembourg. (Oral communication)

66. Campos, A, Puerto, M, Prieto, A, Cameán, A, Almeida, AM, Coelho, AV, Vasconcelos, V. 2010. Analysis of protein expression in bivalve species exposed to the cyanobacteria Cylindrospermopsis raciborskii. 4th EUPA meeting and 6th PROCURA Meeting, 23-27 October, Estoril, Portugal.

67. Campos, A, Puerto, M, Prieto, A, Cameán, A, Almeida, AM, Coelho, AV, Vasconcelos, V. 2010. Two-dimensional gel electrophoresis for analysis of protein expression in Mytilus galloprovincialis. 4th EUPA meeting and 6th PROCURA Meeting, 23-27 October, Estoril, Portugal.

68. Campos, AM, Puerto, MP, Prieto, AI, Cameán, AM, Vasconcelos, VM. 2010. Two-dimensional gel electrophoresis of proteins for functional proteomics in bivalve species. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

69. Campos, C, Conceição, L, Valente, LMP, Fernandes, JMO. 2010. Incubation temperature affects methyltransferases expression during ontogeny of Senegalese sole (Solea senegalensis). EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. Book of abstracts, pp. 270-271.

70. Campos, C, Conceição, L, Valente, LMP, Fernandes, JMO. 2010. Methyltransferases in ontogenic thermal plasticity in Senegalese sole. 9th International Congress on the Biology of Fish of the American Fisheries Society, 5-8 July, Barcelona, Spain. (Oral communication)

71. Campos, C, Conceição, LEC, Valente, LMP, Fernandes, JMO. 2010. Thermal plasticity of methyltransferases expression during ontogeny of Senegalese sole. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain. (Oral communication)

72. Cancela, ML. 2010. Gla Proteins as calcification inhibitors. ESF Exploratory workshop on Microcrystal-induced inflammation and joint diseases in humans, 11-12 March, Paris, France. (Invited oral communication)


294

73. Canning-Clode, J, Maloney, K, McMahon, S, Wahl, M. 2010. Integrating succession and functional richness in the local-regional richness relationship - a global scale perspective. 39th Benthic Ecology Meeting, 10-13 March, Wilmington, NC, USA. (Oral communication)

74. Canning-Clode, J. 2010. Fouling community recruitment, local vs. regional species pools. ICES Benthic Ecology Working Group Meeting 2010, 19-23 April, Edgewater, Maryland, USA. (Invited oral communication)

75. Capela, RC, Silva, HC, Silva, DM, Hamer, PA, Nunes, B, Correia, AT. 2010. Trace elemental incorporation in otoliths in otoliths of the European eel (Anguilla Anguilla): the potential use of a chronological indicator of environmental aquatic exposure to metal contamination. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain.

76. Cardeira, J, Mendes, AC, Pousão-Ferreira, P, Cancela, ML, Gavaia, PJ. 2010. Characterization of lordosis-kyphosis vertebral deformities in juvenile Senegalese sole (Solea senegalensis, Kaup 1858) by immuno-histochemical techniques. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal.

77. Cardeira, J, Nicolau, L, Pousão-Ferreira, P, Cancela, ML, Gavaia, PJ. 2010. Early skeletal development of meagre (Argyrosomus regius, Asso 1801). International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal.

78. Cardeira, S, Cravo, A, Relvas, P. 2010. Chlorophyll a and nutrients fluxes and transports in the south Portuguese coast. XV Iberian Seminar of Marine Chemistry, 22-24 February, Vigo, Spain. (Oral communication)

79. Cardinal, M, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Hussenot, J, Rocha, E, Malhão, F, Escórcio, C, Bacelar, M, Valente, LMP. 2010. Product quality according to the rearing system: Intensive / semi-Intensive / Extensive / Integrated system. Model species: sea bream (Sparus aurata). International Workshop on sustainable extensive and semi-intensive coastal aquaculture in southern Europe, 20-21 January 2010, Tavira, Portugal. (Oral communication)

80. Cardoso, JFMF, Witte, JIJ, Veer, HW. 2010. Differential reproductive strategies of two bivalves in the Dutch Wadden Sea. Annual Netherlands Ecology Meeting, 9-10 February, Lunteren, The Netherlands. (Oral communication)

81. Carrola, J, Santos, N, Fontaínhas-Fernandes, A, Viegas, I, Rocha, MJ, Monteiro, RAF, Rocha, E. 2010. Frequency of micronuclei and of other nuclear aberrations in erythrocytes of mullets from the Mondego, Douro and Ave estuaries - Preliminary data from a major toxicopathology survey. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

82. Carvalho, F, Murie, D, Hazin, F, Coelho, R, Travassos, P, Hazin, H, Burgess, G. 2010. Elasmobranch catches and discards in the pelagic longline fishery in Brazilian fishing grounds off the Southwest Atlantic Ocean: an overview of the last 32 years. Sharks International, 6-11 June, Cairns, Australia.

83. Carvalho, PN, Evangelista, R, Basto, MCP, Vasconcelos, MTSD. 2010. Can some estuarine salt marsh plants influence distribution and remediation of DDT and its metabolites? 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.


295

84. Castanheira, MF, Martins, CIM, Engrola, S, Conceição, LEC. 2010. Daily oxygen consumption rhythms of Senegalese sole (Solea senegalensis) juveniles. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. Book of abstracts, pp. 295-296.

85. Castro, LFC, Gonçalves, O, Wilson, JM. 2010. Pepsinogen precursor genes in the shark, Scyliorhinus canicula: insights into pepsinogen evolution. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.

86. Castro, LFC, Lima, D, Nishikawa, J-I, Hiromori, Y, Nakanishi, T, Fonseca, R, Antunes, A, Mazariegos, JG, Campo-Paysaa, F, Machado, A, Reis-Henriques, MA, Laudet, V, Schubert, M, Santos, MM. 2010. Ancient bilaterian retinoic acid signaling: characterization of the Retinoic Acid receptor (RAR) from the lophotrochozoan mollusk Nucella lapillus. 25th Conference of European Comparative Endocrinologists, 31 August-4 September, Pécs, Hungary. (Oral communication)

87. Cavaleiro, FI, Santos, MJ. 2010. The pattern of site selection of Acanthochondria cornuta (Copepoda: Poecilostomatoida: Chondracanthidae) in the European flounder, Platichthys flesus (Teleostei: Pleuronectidae). 1st International Workshop on Symbiontic Copepoda, 4-8 December, San Pedro, California, USA. (Oral communication)

88. Cavaleiro, FI, Santos, MJ. 2010. What do we know about the octopicolid copepods? 1st International Workshop on Symbiontic Copepoda, 4-8 December, San Pedro, California, USA.

89. Cavenati, S, Carvalho, PN, Almeida, CMR, Basto, MCP, Vasconcelos, MTSD. 2010. A simple method for quantification of tetracyclines in wastewater samples. 3rd EuCheMS Chemistry Congress, 29 August-2 September, Nürnberg, Germany.

90. Cianca, RCC, Baptista, MS, Lopes, VR, Vasconcelos, VM. 2010. Validation of a Reversed-phase

HPLC/FD Method for β-methylamino-L-alanine quantitative analysis using methanol elution instead of acetonitrile. 14th International Conference on Harmful Algae, 1-5 November, Crete, Greece.

91. Cipollini, P, Barbosa, S, Coelho, H, Fernandes, J, Gommenginger, C, Gómez-Enri, J, Martin-Puig, C, Vignudelli, S, Woodworth, P, Benveniste, J, Bastos, L, Byfield, V, Challenor, P, Dinardo, S, Gleason, S, Lázaro, C, Lucas, BM, Moreno, L, Nunes, A, Pires, N, Quartly, G, Snaith, H, Tsimplis, M, Wolf, J. 2010. A summary of the COASTALT project and its contribution to the monitoring of coastal sea level. EGU 2010 General Assembly, 2-7 May, Vienna, Austria.

92. Coelho, R, Santos, MN, Hazin, F, Carvalho, F, Fernandez-Carvalho, J, Burgess, G. 2010. Trans-Atlantic pelagic sharks research initiative 2010. ICES Annual Science Conference, 20-24 September, Nantes, France.

93. Colen, R, Rodrigues, V, Conceição, L, Dinis, MT, Ribeiro, AR, Escórcio, C, Valente, LMP, Aires, T, Dias, J. 2010. Growth performance of gilthead seabream (Sparus aurata) juveniles fed a low fishmeal diet. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. Book of abstracts, pp. 327-328.

94. Conceição, LEC, Aragão, C, Dias, J, Costas, B, Terova, G, Martins, C, Tort, L. 2010. Modulation of fish welfare through adequate dietary nitrogen formulations. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. (Oral communication)

95. Conceição, LEC, Aragão, C, Rønnestad I. 2010. Protein metabolism and amino acid requirements in fish larvae. X Simposio Internacional de Nutrición Acuícola, 8-10 November, San Nicolás de los Garza, Nuevo León, México. (Oral communication)


296

96. Conceição, LEC, Cordeiro, O, Alves, RN, Silva, TS, Richard, N, de Vareilles, M, Rodrigues, PM. 2010. Proteome expression as a tool to identify stress indicators in farmed fish. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain. (Oral invited communication)

97. Conceição, LEC. 2010. How to reduce variability in operational results: specific issues in extensive and semi-intensive systems. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal. (Oral communication)

98. Cordeiro, OD, Silva, TS, Alves, RN, Costas, B, Wulff, T, Richard, N, de Vareilles, M, Conceição, LEC, Rodrigues, PM. 2010. Changes in the liver proteome of Senegalese sole in response to repeated handling stress: the unfolded protein response. 4th EUPA Meeting and 6th PROCURA Meeting, 23-27 October, Estoril, Portugal.

99. Correia, AT, Carocinho, B, Erzini, K, Hamer, PA, Silva, A. 2010. Population structure of the sardine (Sardina pilchardus) inferred from otolith elemental analysis. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain. (Oral Communication)

100.Correia, AT, Pipa, P, Gonçalves, JMS, Erzini, K, Hamer, P. 2010. Assessing the population structure of the common two-banded sea bream (Diplodus vulgaris) at the SW Portuguese coast using otolith elemental fingerprinting. 9th Internacional Congress on the Biology of Fish - Symposium Fish Habitat: improving connectivity, 5-9 July, Barcelona, Spain. (Oral Communication)

101.Correia, AT, Pipa, P, Gonçalves, JMS, Erzini, K, Hamer, P. 2010. New insights about the life cycle of Diplodus vulgaris at the SW Portuguese coast using otolith fingerprint analysis. 45th European Marine Biology Symposium, 23-27 August, Edinburgh, United Kingdom. (Oral Communication)

102.Costa, A, Mil-Homens, M. 2010. Distribuição granulométrica dos sedimentos de superfície do estuário do rio Minho. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal.

103.Costa, R, Esteves, AI, Hardoim, CCP. 2010. The Microbial Ecology and Evolution Research Group at the Centre of Marine Sciences, Algarve, Portugal. Microbial Genomics & Metagenomics Workshop, 18-22 October, Walnut Creek, CA, USA.

104.Costa, R, Keller, T, Nunes da Rocha, U, Xavier, J, Van Overbeek, L, Van Elsas, JD. 2010. 16S rRNA gene-based analysis of bacterial diversity and composition in the freshwater sponge Ephydatia fluviatilis. VIII World Sponge Conference, 20-24 September, Girona, Spain.

105.Costa, R. 2010. Community structure and function in “hot spots” of microbial activity. 2010. The International Fluorescent in Situ Hybridization (FISH) Course, 22-26 February, Vienna, Austria. (Oral communication)

106.Costa, R. 2010. Unveiling the Zostera marina microbiome. Seagrass Workshop, 6-9 December, Virginia Institute of Marine Science, Williamsburg, Virginia, USA. (Oral communication)

107.Costas, B, Rêgo, PC, Conceição, LEC, Dias, J, Afonso, A. 2010. Effects of dietary arginine on the stress response and some haematological and immune parameters of turbot (Schophthalmus maximus). 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain. (Oral communication)

108.Costas, B, Silva, JMG, Conceição, LEC, Dias, J, Valente, LMP, Afonso, A. 2010. Influence of fish meal replacement by plant-protein sources and indispensable amino acids supplementation on innate immune parameters of Senegalese sole Solea senegalensis. EAS Aquaculture Europe 2010


297

- Seafarming tomorrow, 5-10 October, Porto, Portugal. Book of abstracts, pp. 335-336. (Oral communication)

109.Couto, A, Enes, P, Peres, H, Oliva-Teles, A. 2010. Growth performance of European sea bass (Dicentrarchus labrax) fed diets containing whole cereal meals. EAS Aquaculture Europe 2010 - Seafarming tomorrow, 5-10 October, Porto, Portugal.

110.Couto, CMCM, Pinto, I, Madureira, TV, Rocha, MJ, Tiritan, ME, Almeida, AA. 2010. Spatio-temporal distribution of metals in the Douro River estuary (Portugal). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

111.Couto, CMCM, Pinto, I, Madureira, TV, Rocha, MJ, Tiritan, ME, Almeida, AA. 2010. Evaluation on the influence of WTPs on estuarine water quality – anthropogenic influences on metal content on surface waters of the Douro estuary (Portugal). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

112.Couto, RP, Neto, AI, Rodrigues, AS. 2010. Limpet shell modifications at intertidal hydrothermal vents. 45rd European Marine Biology Symposium, 23-27 August, Edinburgh, Scotland. Book of abstracts, p.60.

113.Couto, RP, Neto, AI, Rodrigues, AS. 2010. Metal concentrations and structural changes in coralline algae from hydrothermal vents. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.53. (Oral communication)

114.Couto, RP, Rodrigues, AS, Neto, AI. 2010. Coralline algae, structuring organisms of warm temperate intertidal communities. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.64.

115.Couto, RP, Rosas-Alquicira, EF, Rodrigues, AS, Neto, AI. 2010. Choreonema thuretii and Pneophyllum confervicolum (Corallinales, Rhodophyta), new corallines to the Azores. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.39.

116.Cox, CJ. 2010. Investigating the archaebacterial origin of eukaryotes. Annual Meeting of the Society of Molecular Biology and Evolution - SMBE 2010, 4-8 July, Lyon, France. (Invited oral communication)

117.Crans, DC, Trujillo, AM, Winter, PW, Roess, D, Aureliano, M. 2010. Membrane interactions

impacting the toxic and beneficial effects of vanadium-containing oxometalates. International Chemical Congress of Pacific Basin Societes - PACIFICHEM 2010, 15-20 December, Hawaai, USA (Oral communication).

118.Cristiano, MLS, Ismael, A, Frija, L, Fausto, R, Reva, I. 2010. Isomerisations in heteroaromatic allyl ethers: selectivity and mechanism. 20th International Conference on Physical Organic Chemistry, 22-28 August, Busan, South Korea. (Oral communication)

119.Cristiano, MLS. 2010. Applications of antimalarial endoperoxide drugs to aquaculture. 2nd UTEN Workshop 2010, Marine and Bio-Sciences, 27-28 September, Faro, Portugal. (Invited oral communication)


298

120.Cristiano, MLS. 2010. Design and synthesis of fluorescent compounds derived from artemisinin, deoxyartemisinin and ozonide; suitability of the compounds as probes of embriotoxicity and teratogenicity. 6 May, University of Gothenburg, Sweden. (Invited oral communication)

121.Cristiano, MLS. 2010. Photochemistry of tetrazoles; applications in organic synthesis. 2nd Iberoamerican Symposium of Organic Chemistry, 8-11 September, Santiago de Compostela. (Invited oral communication)

122.Cristiano, MLS. 2010. Synthetic approaches to biotin-tagged derivatives of artemisinin, deoxyartemisinin and ozonide to be used as affinity probes of embriotoxicity and teratogenicity. 20 October University of Leiden, The Netherlands. (Invited oral communication)

123.Cruz, C, Silva, A, Vaz, A, Luís, L, Hermida, M, Saraiva, A. 2010. Ocorrência de Kudoa spp. (Myxozoa, Multivalvulida) em peixes marinhos usados para consumo humano em Portugal. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil. (Oral communication)

124.Cruzeiro, L, Lopes, PA. 2010. Parallel computing and protein design. 4th Iberian Grid Infrastructure Conference Proceedings, 24-27 May, Braga, Portugal. (Oral communication)

125.Cruzeiro, L. 2010. A kinetic mechanism for protein folding in a multi-funnel free energy landscape. IV Spanish-Portuguese Biophysical Congress 2010, 8-10 July, Zaragoza, Spain. (Oral communication)

126.Cruzeiro, L. 2010. The VES Hypothesis and Protein Function. 1st Porto Meeting on Theory and Experiments in Nonlinear Physics, 7-9 July, Porto, Portugal. (Oral communication)

127.Cunha, AH Assis, J, Tavares, D, Tavares, JT, Paulos, L, Serrão, E, Erzini, K. 2010. Adopt-a-Seagrass-Meadow: a people´s conservation program. World Seagrass Biology Conference and International Seagrass Workshop 2010, 22-30 November, Phuket and Trang, Thailand.

128.Cunha, AH, Erzini, K, Serrão, E, Gonçalves, E, Henriques, M, Henriques, V, Guerra, M, Duarte, C, Marbá, N, Fonseca, M. 2010. Biomares a LIFE project to restore and manage the biodiversity of Luiz Saldanha Marine Park. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain. (Oral communication)

129.Cunha, AH, Paulo, D, Rodrigues, S, Boavida, J, Fonseca, M. 2010. Open ocean restoration in the eastern Atlantic coast: challenges and lessons. I European Seagrass Restoration Workshop, 30 September, Portinho da Arrábida, Portugal. (Oral communication)

130.Cunha, AH, Paulo, D, Rodrigues, S, Boavida, J, Fonseca, M. 2010. Open ocean restoration in the eastern Atlantic coast: challenges and lessons. World Seagrass Biology Conference and International Seagrass Workshop, 22-30 November, Phuket and Trang, Thailand. (Oral communication)

131.Cunha, AH, Serrão, E, Assis, J. 2010. Seagrass Status of Portuguese Seagrasses: are seagrasses the most endangered marine habitat? International Coastal Conservation Conference - ICCCM, 12-17 April, Estoril, Portugal. (Oral communication)

132.Cunha, AH, Serrão, E, Assis, J. 2010. Seagrass Status of Portuguese Seagrasses: are seagrasses the most endangered marine habitat? ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)


299

133.Cunha, I, Galante-Oliveira, S, Rocha, E, Castro, FL. 2010. Effects of treatment with the standard PPARa ligand WY14643 on juvenile turbot Scophthalmus maximus - II. Gene expression. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

134.Cunha, I, Galante-Oliveira, S, Rocha, E, Castro, LFC. 2010. Pattern of expression of PPARα1/α2 and other genes involved on lipid metabolism on post-hatched turbot (Scophthalmus maximus) larvae. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

135.Custódio, L, Barradas, AR, Justo, T, Barreira, L, Varela, J. 2010.Evaluation of the antioxidant potential of selected species of microalgae. 8th European Workshop of Microalgal Biotechnology, 7-10 June, Nuthetal, Germany.

136.Custódio, L, Justo T, Vizetto-Duarte, C, Pereira, H, Silvestre, L, Barreira, L, Rauter, AP, Alberício, F, Varela, J. 2010. Chelating and acetylcholinesterase inhibitory activity of selected species of microalgae. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal. (Oral Communication).

137.da Silva, JCB, Grimshaw, RHJ, Magalhaes, JM. 2010. Observations of internal solitary wave reflection at a step-like submarine bank and strong oblique interaction at Race Point Channel, (Cape Cod). European Geophysical Union - EGU General Assembly 2010, 2-7 May, Vienna, Austria. (Oral communication)

138.da Silva, JCB, New, AL, Helfrich, KR, Pineda, J, Ostrowski, M, Basik-Sangolay, B, Magalhaes, JM, Azevedo, A. 2010. Internal solitary waves in the world’s ocean and some mechanisms of their formation. European Space Agengy SEASAR 2010 Workshop, 25-29 January, Frascati, Italy. (Oral communication)

139.de la Riva, GA, Agüero-Chapin, G, Morales-Aragón, JC, Pérez-Machado, G, Vasconcelos, V,

Antunes, A. 2010. Comparative enzymatic activity of three recombinant RNAse III and effect of

bacterial RNAse III expressed in transformed yeasts. 3th International Research Congress CIPITECH 2010, 13-15 November, Instituto Tecnológico de Parral, Mexico. (Oral communication)

140.de Vareilles, M, Gomèz-Requeni, P, Kousoulaki, K, Cordeiro, O, Silva, TS, Richard, N, Conceição, LEC, Rodrigues, PM, Rønnestad, I. 2010. Growth and muscle proteome response to fish protein hydrolysates in the diet of zebrafish. 14th International Symposium on Fish Nutrition and Feeding, 31 May-4 June, Qingdao, China.

141.de Vareilles, M, Gómez-Requeni, P, Kousoulaki, K, Richard, N, Cordeiro, O, Silva, TS, Rodrigues, PM, Conceição, LEC, Rønnestad, I. 2010. Growth and muscle proteome response to a dietary lysine unbalance in zebrafish juveniles. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 360-361.

142.de Vareilles, M, Gómez-Requeni, P. Kousoulaki, K, Richard, N, Cordeiro, O, Silva, TS, Rodrigues, PM, Conceição, LEC, Rønnestad, I. 2010. Growth and muscle proteome response to a dietary lysine unbalance in zebrafish juveniles. 14th International Symposium on Fish Nutrition and Feeding, 31 May-4 June, Qingdao, China.

143.Desprat, S, Sanchez Goñi, MF, Naughton, F, Turon, J-L, Malaizé, B. 2010. Interglacial climatic variability in southern Europe from NW Iberian marine pollen records. American Geophysical Union - AGU Fall Meeting, 5-9 December, San Francisco, California, USA.


300

144.Dias, J, Conceição, LEC, Ramalho Ribeiro, A, Aires, T, Borges, P, Valente, L, Rema, P, Dinis, MT. 2010. Diet formulation for sustainable fish farming in ponds/lagoons. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January,

Tavira, Portugal. (Oral communication)

145.Dias, J, Rema, P, Verlhac, V. 2010. Protease supplementation enhances the apparent digestibility of nutrients and energy in Nile tilapia fed plant-protein rich diets with variable crude protein content. 14th International Symposium on Fish Nutrition and Feeding, 31 May-4 June, Qingdao, China.

146.Dias, J, Rodrigues, V, Colen, R, Bandarra, N, Cancela, ML, Laizé, V, Gavaia, PJ. 2010. Effects of dietary lipid sources on bone composition and metabolism in gilthead seabream (Sparus aurata) juveniles. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Oral communication)

147.Dias, J, Yúfera, M, Valente, LMP, Rema, P. 2010. Feed transit and apparent protein, phosphorus and energy digestibility of practical feed ingredients by Senegalese sole (Solea senegalensis). EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 373-374.

148.Dias, ML, Souza, GTR, Eiras, JC, Machado, MH, Pavanelli, GC, Silva, ES. 2010. Desenvolvimento de metacercárias de Clinostomum complanatum Rud. 1814 (Digenea: Clinostomidae) a adultos ovígeros em hospedeiro experimental. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil.

149.Dini-Andreote, F, Andreote, FD, Costa, R, Taketani, RG, Van Elsas, JD, Araújo, WL. 2010. The bacterial soil community in a Brazilian sugar cane field. 13th International Symposium on Microbial Ecology, 22-27 August, Seattle, USA.

150.Diogo, P, Soares, F, Beirão, J, Dinis, MT, Cabrita, E. 2010. Parameters affecting sperm motility activation in Solea senegalensis: multivariate cluster analysis vs mean values analysis. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

151.Dionísio, G, Cunha, ME, Ferreira, H, Makridis, P, Cancela, ML, Gavaia, PJ. 2010. Effect of two culture conditions on growth and skeletogenesis of Solea senegalensis early life stages. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

152.Dionísio, G, Richard, N, Bensimon-Brito, A, Cancela, ML, Gavaia, PJ. 2010. Effect of dietary vitamin K supplementation on growth and skeletal development of two teleost models. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal. (Oral communication)

153.Dolbeth, M, Sousa, R, Cusson, M, Pardal, MA. 2010. Secondary invertebrate production as a tool to better understand the aquatic ecosystem. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)

154.Duarte, H, Silva, A, Mil-Homens, M, Borges, R, Lopes, C, Santos, C, Cavaleiro, C, Mateus, S, Lopes, AL. 2010. Bedforms and sediment distribution in the Minho estuary (Northern Portugal). European Geophysical Union - EGU General Assembly, 2-7 May, Vienna, Austria.

155.Duarte, P, Pereira, A, Reis, LP, Labarta, U, Fernandéz-Reiriz, M, Cranford, P. 2010. Carrying capacity modelling combined with decision support tools. Workshop – Networking Research on Tools to Support Decision Making and to Develop Sustainability in Shellfish Aquaculture Management and Exploitation, 5th October, Porto, Portugal. (Oral communication)


301

156.Duarte, P, Pereira, A., Reis, LP. 2010. An integrated ecological modeling and decision support methodology for coastal ecosystem analysis and management. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)

157.Duarte, S, Fidalgo, ML, Pascoal, C, Cássio, F. 2010. Food preferences of the freshwater shrimp Atyaephyra desmarestii. XV Congress of the Iberian Association of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. (Oral communication)

158.Einarsdóttir, IE, Jönsson, EB, Thrandur Björnsson, BTh, Power, DM. 2010. Distribution of ghrelin-producing cells and Na+K+-ATPase in tissues of atlantic halibut (Hippoglossus hippoglossus) in early development. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

159.El Khalloufi, F, El Ghazali, I, Saqrane, S, Ouahid, Y, Del Campo, FF, Vasconcelos, V, Oudra, B. 2010. Induction of necrosis of the tomato plant leaves exposed to microcystins. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

160.El Khalloufi, F, Lahrouni,

M, El Ghazali, I, Oufdou, Kh, Vasconcelos, VM, Oudra, B. 2010. Contamination of irrigation water by microcystins: Effects on the potential symbiotic association between rhizobia and Medicago sativa. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

161.Engelen, A, Diekmann, O, Coelho, M, Costa, J, Pearson, G, Marbà, N, Serrão, E, Duarte, C. 2010.

Climate change effects on canopy-forming ecosystem structuring species at leading and rear edges versus central distribution zones. AQUASHIFT Workshop - The Impact of Climate Variability on Aquatic Ecosystems, 4-7 October, Kiel, Germany.

162.Engelen, A, Lévèque, L, Engel, C, Viard, F, Destombe, C, Valero, M. 2010. Unraveling population dynamics of Laminaria digitata using matrix models: life history and management strategies. Ecokelp International workshop on Algal Resource Management, 3-5 June, Brest, France. (Oral communication)

163.Engrola, S, Cabrita, E, Teixeira, H, Conceição, LEC, Pousão-Ferreira, P, Dinis, MT. 2010. Female performance of dusky grouper Epinephelus marginatus Lowe 1854) during consecutive years. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 418-419.

164.Erzini, K. 2010. Performance indicators for the Professor Luíz Saldanha Marine Park: a multi-criteria approach. MAIA Internacional Workshop - MPAs Monitoring strategies, 8-9 November, Sesimbra, Portugal. (Oral communication)

165.Estrada-Allis, SN, Caldeira, RMA, Rodríguez-Santana, Á, Sangrà, P, Machín, F, Couvelard, X. 2010. Hacia la construcción de un modelo regional en el Sistema de Afloramiento de la Península Ibérica (IPUS). EOF – Spanish Workshop on Physical Oceanography, 13-15 October, Barcelona, Spain. (Oral communication)

166.Fathalli, A, Jenhani, A, Moreira, C, Antunes, A, Welker, M, Romdhane, M, Vasconcelos, V.

2010.

Toxicity assessment and phylogenetic characterization of Microcystis strains isolated from Tunisian freshwaters. 8th International Conference on Toxic Cyanobacteria. 29 August-4 September, Istambul, Turkey.

167.Fernandes, J, Campos, C, Valente, L. 2010. Molecular evolution of zebrafish Dnmt3 genes and differential expression in relation to embryonic temperature. SEB Annual Main Meeting 2010, 30 June-3 July, Prague, Czech Republic. (Oral communication)


302

168.Fernandes, MJ, Benveniste, J, Berry, P, Cipollini, P, Coelho, E, Collard, F, Deng, X, Emery, W, Fenoglio-Marc, L, Gommenginger, C, Ichikawa, K, Saraceno, M, Scharroo, R, Smith, W, Strub, T, Vandermark, D, Vignudelli, S, Wilkin, J, Woodworth, P. 2010. Report on the 4th Coastal Altimetry Workshop, 18-20 October, OSTST 2010, Lisbon, Portugal. (Keynote communication)

169.Fernandes, MJ, Lázaro, C, Nunes, A, Pires, N, Cipollini, P. 2010. Tropospheric corrections for coastal altimetry. ESA Living Planet Symposium, 28 June-2 July, Bergen, Norway. (Oral communication)

170.Fernandes, MJ, Lázaro, C, Nunes, A, Pires, N, Salgado, M, Cipollini, P. 2010. GPD – towards a global implementation. 4th Workshop on Coastal Altimetry, 14-15 October, Porto, Portugal. (Oral communication)

171.Fernandes, MJ, Lázaro, C, Nunes, A, Pires, N, Salgado, M, Cipollini, P. 2010. Inter-comparison between existing wet tropospheric corrections for coastal altimetry. OSTST 2010, 18-20 October, Lisbon, Portugal.

172.Fernandez-Carvalho, J, Coelho, R, Santos, MN. 2010. Age and growth of the big eye thresher shark, Alopias superciliosus, in the Eastern Atlantic Ocean. ICES Annual Science Conference 2010, 20-24 September, Nantes, France.

173.Ferreira, C, Reis, B, Figueiredo, F, Cruzeiro, C, Rocha, E, Rocha, MJ. 2010. Regular chemical monitoring reveals continuous water pollution by estrogenic endocrine disruptor compounds in the Douro River estuary, Porto, Portugal. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

174.Ferreira, H, Ramalho Ribeiro, A, Dias, J, Yúfera, M, Arias, A, Falcão, M, Serpa, D, Aires, T, Pousão-Ferreira, P, Cunha, ME, Valente, LMP, Dinis, MT, Conceição, LEC. 2010. Sustainable semi-intensive polyculture of seabream and sole in earth ponds. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira,

Portugal. (Oral communication)

175.Ferreira, H, Ribeiro, AR, Dias, J, Yúfera, MI, Arias, A, Falcão, M, Serpa, D, Aires, T, Pousão-Ferreira, P, Cunha, ME, Valente, LMP, Dinis, MT, Conceição, L. 2010. Sustainable Semi-intensive polyculture of seabream and sole in earth ponds. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 453-454. (Oral communication)

176.Ferreira, M, Zaja, R, Reis-Henriques, MA, Moradas-Ferreira, P, Smital, T. 2010. CYP1A activity and the ABC proteins(s) mediated multixenobiotic resistance in european bass (Dicentrarchus labrax) primary cultured hepatocytes. 6th. International Conference on Marine Pollution and Ecotoxicology, 31 May-3 June, Hong Kong, China. (Oral Communication)

177.Ferrette, BLS, Mendonça, FF, Oliveira, C, Gadig, OBF, Coelho, R, Foresti, F, Santos, MN 2010 Determination of multiple paternity in the crocodile shark (Pseudocarcharias kamoharai) in the Equatorial Atlantic Ocean. 56th Brazilian Congress of Genetics, 14-17 September, Guarujá, São Paulo, Brazil.

178.Figueiredo, F, Ribeiro, M, Ferreira, C, Reis, B, Cruzeiro, C, Rocha, E, Rocha, MJ. 2010. Measurement of several classes of pesticides by GC-MS in the Douro River estuary, Porto, Portugal. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.


303

179.Figueiredo-Silva, AC, Corraze, G, Kaushik, S, Peleteiro, JB, Valente, LMP. 2010. Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 461-462.

180.Figueiredo-Silva, AC, Corraze, G, Valente, LMP. 2010. Recent advances and future steps concerning blackspot seabream (Pagellus bogaraveo) nutrition. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 463-464.

181.Franklin, CE, Reilly, BD, Cramp, RL, Wilson, JM. 2010. Ion transporters in the gills of the euryhaline bull shark: effect of environmental salinity. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain. (Oral communication)

182.Frazão, B, Martins, R, Vasconcelos, V. 2010. Are known cyanotoxins involved in the toxicity of picoplanktonic and filamentous North Atlantic marine cyanobacteria? VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica.

183.Freedman, H, Cruzeiro, L. 2010. Amide I vibration within an MD simulation. CECAM Workshop on Quantum Coherence, 9-12 May, Dublin, Ireland.

184.Freedman, H, Martel, P, Cruzeiro, L. 2010. Quantum-classical molecular dynamics simulation. CECAM Workshop on Protein Folding Dynamics, 4-7 October, Lausanne, Switzerland.

185.Freitas, F, Mota, M, Nogueira, AJA, Antunes, C, Sousa, R. 2010. Distribution of the invasive crayfish Procambarus clarkii in the international section of the River Minho (NW of the Iberian Peninsula). V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

186.Freitas, M, Azevedo, J, Vasconcelos, V. 2010. Biodisponibilidade de cianotoxinas em alimentos e implicações para a saúde humana. I International Health Congress Gaia-Porto, 23-25 September, Vila Nova de Gaia, Portugal. (Oral communication)

187.Freitas, M, Martins, A, Moreira, C, Azevedo, J, Vasconcelos, V. 2010. Monitorização de cianobactérias e cianotoxinas: métodos e volumes de amostragem. I International Health Congress Gaia-Porto, 23-25 September, Vila Nova de Gaia, Portugal. (Oral communication)

188.Freitas, M, Martins, A, Regueiras, A, Azevedo, J, Moreira, C, Vasconcelos, V. 2010. Monitoring of cyanobacteria and cyanotoxins in Mira and Vela lakes (Central Portugal) comparing different methods and sample volumes. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

189.Gadelha, J, Von Osten, J, Guilhermino, L, Abreu, S, Soares, A, Morgado, F. 2010. Sea anemones Actinia equina and Anemonia sulcata as bioindicators of persistent organic pollutants (POPs) bioaccumulation in the Iberian Coast. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain. Book of abstracts, p.226.

190.Galante-Oliveira, S, Castro, LFC, Rocha, E, Cunha, I. 2010. Developmental toxicity of fibrates on turbot (Scophthalmus maximus) embryos: effects on mortality, growth, use of vitelline reserves and malformation rates. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.


304

191.Galante-Oliveira, S, Resende, D, Fontaínhas-Fernandes, A, Rocha, E, Cunha, I. 2010. Effects of treatment with the standard PPARa ligand WY14643 on juvenile turbot Scophthalmus maximus - I. Biochemical parameters. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

192.Gama, M, Guilhermino, L, Canhoto, C. 2010. Effects of temperature and food quality on the ecology, elemental composition and biomarkers of Echinogammarus meridionalis: a preliminary approach. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain. Abstract book, p.121.

193.García, S, Garrido, D, Rosas, C, Sykes, AV, Domingues, P. 2010. Characterization of the enzymatic activity in the gastric juice and digestive gland of Octopus (Octopus vulgaris) and Cuttlefish (Sepia officinalis) at different pH and temperatures. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

194.García-Marín, P, Hernández, I, Cabaço, S, Brun, FG, Silva, J, Santos, R, Vergara, JJ. 2010. Seagrass Indicator for South Iberian Peninsula (SISIP): first steps to fulfill the WFD requirements. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. (Oral communication)

195.García‐Marín, P, Hernández, I, Cabaço, S, Brun, FG, Silva, J, Santos, R, Vergara, JJ. 2010. Seagrass indicators in the southern Iberian Peninsula: first steps to fulfilling WFD requirements. ICES Annual Science Conference, 20-24 September, Nantes, France.

196.Garel, E, Barbosa, AB, Bhaskar, P, Chícharo, MA, Cravo, A, Ferreira, O. 2010. Multi-parametric signature of the lower Guadiana Estuary from 2-year long continuous monitoring. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal.

197.Garrido, S, Cristóvão, AR, Palma, S, Baptista, H, Nunes, G, Lobo, V, Santos, E, Baylina, N, Gordo, L, Chícharo, MA, Santos, AMP, Saiz, E, Ré, P. 2010. Reproduction of the sardine Sardina pilchardus in laboratory conditions effect of food availability on the survival, development and growth of first-feeding larvae Nova. 9th Larval Biology Symposium, 23-27 August, Wellington, New Zeeland.

198.Gavaia, PJ, Cunha, ME, Ferreira, H, Pousão-Ferreira, P, Cancela, ML. 2010. Early skeletal development of the dusky grouper (Epinephelus marginatus, Lowe), produced under extensive mesocosms conditions. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

199.Gavaia, PJ, Richard, N, Dâmaso, L, Dinis, MT, Pousão-Fereira, P, Engrola, S, Conceição LEC, Cancela, L. 2010. Evaluation of the skeletal quality in Senegalese sole (Solea senagalensis) reared under intensive vs extensive conditions. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira, Portugal.

200.Gavaia, PJ. 2010. Ossification and Malformations. LARVITA Fish Larvae Training School. COST action FA0801, 22-26 November, Faro, Portugal. (Oral communication)

201.Gil, IM, Keigwin, L, Abrantes, F. 2010. Deglacial diatom record from the Laurentian Fan (South of Newfoundland) reveals the tempo of the ice formation and dispersion. 10th International Conference on Paleoceanography, 29 August-3 September, San Diego, USA.

202.Gonçalves, A, Bandarra, N, Dias, J, Nunes, ML. 2010. Effect of diet with low fish-derived protein and oil on the sensory properties and nutritional value of gilthead sea bream (Sparus aurata). International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira, Portugal. (Oral communication)


305

203.Gonçalves, A, Matos, E, Dias, J, Nunes, ML. 2010. Effect of the replacement of dietary fishmeal and fish oil by vegetable ingredients on the sensory quality of farmed gilthead sea bream (Sparus aurata). 4th European Conference on Sensory and Consumer Research, 5-8 September, Vitoria, Spain.

204.Gonçalves, AF, Rodrigues, P, Coimbra, J, Wilson, JM. 2010. Effects of ammonia on bacterial lipopolysaccharide induction of the acute phase response in Danio rerio. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain. (Oral communication)

205.Gonçalves, H, Gonçalves, JA, Corte-Real, L. 2010. The use of similarity images on multi-sensor automatic image registration. ISPRS TC VII Symposium - 100 Years ISPRS, 5-7 July, Vienna, Austria. (Oral communication)

206.Gonçalves, J, Café, C, Ozório, ROA, Marques, M, Correia, N, Gomes, R, Fernandes, F, Teles, T, Calheiros, J, Bordalo, AA. 2010. A novel floating waste treatment plant for inland cage aquaculture. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Oral communication)

207.Gonçalves, JA, Bastos, L, Perez, B, Magalhães, A. 2010. Monitoring of beaches and sand dunes using digital aerial photography with direct georeferencing. ISPRS TC VII Symposium - 100 Years ISPRS, 5-7 July, Vienna, Austria. (Oral communication)

208.Gonçalves, JA. 2010. Automatic image orientation and DSM extraction from ALOS-PRISM triplet images. 2010 IEEE International Geoscience and Remote Sensing - IGARSS'10, 25-30 July, Hawaii, USA.

209.Gonçalves, JMS, Bentes, L, Monteiro, P, Rangel, MO, Veiga, P, Afonso, C, Oliveira, F, Erzini, K. 2010. RENSUB – Mapping marine biodiversity for coastal management. 45th European Marine Biology Symposium, 23-27 August, Edinburgh, Scotland.

210.Gonçalves, JMS, Monteiro, P, Oliveira, F, Afonso, C, Veiga, P, Rangel, MO, Erzini, K, Bentes, L. 2010. Coastal management based on mapping and classification of the Algarve marine subtidal communities. International Coastal Conservation Conference - ICCCM, 12-17 April, Estoril, Portugal. (Oral communication)

211.Gonçalves, JMS. 2010. Portuguese experiences with MSP. GISLANDS 2010 International Summer School on Marine Spatial Planning – MSP, 7 August, Ponta Delgada, Azores, Portugal. (Oral communication)

212.Gonçalves, O, Castro, LFC, Smolka, AJ, Wilson, JM. 2010. The cypriniform stomach. A case of reinvention? 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.

213.Gonçalves, O, Castro, LFC, Wilson, JM. 2010. Stomach loss in fishes. A case of déjà vu. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain. (Oral communication)

214.Granja, H, Bastos, L, Pinho, J, Gonçalves, J, Henriques, R, Bio, A, Magalhães, A. 2010. Small harbours risks: lowering for fishery and increasing erosion. The case of Portinho da Aguda (NW Portugal). Littoral 2010, 21-23 September, London, United Kingdom. (Oral communication)

215.Grüneberg, J, Engelen, A, Costa, R, Wichard, T. 2010. Induction of algal morphogenesis in Ulva mutabilis by its associated bacteria in the lagoon Ria Formosa (Portugal). 2nd VAAM Workshop on Symbiotic Interactions, 7-8 October, Würzburg, Germany.


306

216.Guerreiro, I, Peres, H, Oliva-Teles, A. 2010. Effect of dietary protein/lipid level on performance of Senegalase sole (Solea senegalensis) juveniles reared at two different temperatures. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

217.Guerreiro, I, Peres, H, Oliva-Teles, A. 2010. Effect of temperature and dietary protein/lipid ratio on growth performance and feed utilization of juvenile Senegalese sole (Solea senegalensis). International Workshop on Extensive and Semi-intensive Aquaculture Production in Southern Europe, 20-21 January, Tavira, Portugal.

218.Guerreiro, PM, Bataille, AM, Renfro, JL. 2010. The shark choroid plexus actively removes inorganic phosphate from cerebrospinal fluid. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.

219.Guimarães, L, Gravato, C, Santos, J, Faria, M, Alves, A, Guilhermino, L. 2010. Does pollution affects the initial development of glass eels that enter NW Portuguese estuaries? ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. Book of abstracts, p.139.

220.Guimarães, L, Gravato, C, Santos, J, Guilhermino, L. 2010. Impact of pollution on the development of glass eels (Anguilla anguilla) entering three NW Portuguese estuaries. 27th Congress of the European Society of Comparative Biochemistry and Physiology, 5-9 September, Alessandria, Italy.

221.Guimarães, L, Gravato, C, Sousa, A, Guilhermino, L. 2010. Impact of oil-derived pollution on natural populations of P. microps from NW Portuguese estuaries. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

222.Hermida, M, Saraiva, A, Cruz, C. 2010. Dados preliminares sobre a parasitofauna do goraz, Pagellus bogaraveo (Teleostei: Sparidae) na região dos Açores, Atlântico Nordeste. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil.

223.Hernández-Garcia, A, Romero, D, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Effects on enzymatic antioxidant system of mallard (Anas platyrhynchos) erythrocytes exposed in vitro to lead and cadmium. XII International Congress of Toxicology – IUTOX 2010, 19-23 July 2010, Barcelona, Spain.

224.Hernández-Garcia, A, Romero, D, Tagliati, CA, Gravato, C, Guilhermino, L, Garcia-Fernández, AJ. 2010. Glutathione S-transferase activity, glutathione and lipid peroxidation levels in mallard (Anas platyrhynchos) erythrocytes exposed to lead and cadmium in vitro. XII International Congress of Toxicology – IUTOX 2010, 19-23 July 2010, Barcelona, Spain.

225.Herráez, MP, Pérez-Cerezales, S, Martínez-Páramo, S, Beirão, J, Martínez-Pastor, F, Gutiérrez-Adán, A. 2010. Altered gene expression in embryo obtained with DNA-cryodamaged sperm. 1st International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue & Organs, 22-25 April, Valencia, Spain.

226.Horvath, A, Martínez-Páramo, S, Urbanyi, B, Kovacs, A, Herráez, MP. 2010. Cryopreservation of common carp (Cyprinus carpio) sperm in large volumes and subsequent effect of ovarian fluid on sperm activation. 11st International Symposium on Spermatology, 24-29 June, Okinawa, Japan.

227.Hubbard, PC. 2010. Olfactory sensitivity to inorganic cations in fish. ECRO 2010 - European Chemoreception Research organization XXth Congress, 14-19 September, Avignon, France.


307

228.Huertas, M, Canário, AVM, Hubbard, PC. 2010. Olfactory sensitivity to Ca2+ and Na+ in the European eel (Anguilla anguilla). ECRO 2010 - European Chemoreception Research organization XXth Congress, 14-19 September, Avignon, France.

229.Hussenot, J, Cardinal, M, Cariou, S, Fabre, R, Kaas, R, Richard, M, Valente, L, Bruant, J-S. 2010. A fish-macroalgae integrated system to reduce wastes in a land-based marine fish hatchery: a case study in the seacase Project. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 601-602. (Oral communication

230.Hussenot, J, Cardinal, M, Cariu, S, Dupuy, B, Fabre, R, Hamdaoui, M, Hervé, A, Kaas, R, Richard, M, Valente, L, Bruant, JS. 2010. Integrated system versus lagoon/pond treatment as effluent purification process in a land-based marine fish hatchery. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira,

Portugal. (Oral communication)

231.Ibarz, A, Costa, R, Henderson, A, Power, DM. 2010. The intermediate metabolite, alpha ketoglutarate, regulates the pituitary proteome of the gilthead sea bream. 9th International Congress on the Biology of Fish, 5- 9 July, Barcelona, Spain.

232.Ilarri, M, Antunes, C, Guilhermino, L, Sousa, R. 2010. Massive mortality of the Asian clam Corbicula fluminea in a highly invaded area. Aquashift Conference 2010, 4-7 October, Kiel, Germany.

233.Ilarri, M, Freitas, F, Dias, S, Antunes, C, Guilhermino, L, Sousa, R. 2010. The Asian clam Corbicula fluminea as ecosystem engineer: effects on the diversity of benthic macroinvertebrates. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

234.Jalobeanu, A, Gama, C, Gonçalves, JA. 2010. Probabilistic surface change detection and measurement from digital aerial stereo images. IEEE International Geoscience and Remote Sensing - IGARSS'10, 25-30 July, Hawaii, USA. (Oral communication)

235.Jenhani, A, Fathalli, A, Moreira, C, Antunes, A, Romdhane, M, Vasconcelos, V. 2010. Portoamides

– new synergistic allelochemicals from Oscillatoria sp. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

236.Keller, T, Costa, R, Jousset, A, Van Overbeek, L, Van Elsas, JD. 2010. Genotypic and phenotypic characterization of fluorescent Pseudomonas spp. isolated from the freshwater sponge Ephydatia fluviatilis. VIII World Sponge Conference, 20-24 September, Girona, Spain.

237.Keller, T, Iacovella, A Hubbard, PC, Canário, AVM, Barata, EN. 2010. Olfactory responses of male and female Mozambique tilapia (Oreochromis mossambicus Peters 1852) to a putative urinary pheromone that signals male dominance. 26th Annual Meeting of the International Society of Chemical Ecology, 31 July-4 August, Tours, France.

238.Keshin, M, Penna, NT, Clarke, PJ, Bos, MS, Baker, TF. 2010. Anomalous tidal loading signals in South-West England and Brittany. EGU General Assembly, 2-7 May, Vienna, Austria.

239.Keshin, M, Penna, NT, Clarke, PJ, Bos, MS, Baker, TF. 2010. GPS estimation of anomalous tidal loading displacements. International GNSS Service (IGS) 2010 Workshop, 28 June-2 July, Newcastle, United Kingdom.


308

240.Kijjoa, A. 2010. Drugs, nutraceuticals and cosmeceuticals from marine sources – EU Perspectives. Thai Professional Conferences 2010: Green Thailand, 5-7 July 2010, Bangkok, Thailand. (Invited oral communication)

241.Kolbadinejad, S, Hajimoradloo, A, Ghorbani, R, Wilson, JM. 2010. Effects of gradual increase of salinity on some osmoregulatory functions in Caspian Roach, Rutilus rutilus caspicus in the southeast of Caspian Sea, Iran. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.

242.Kolmakov, N, Canário, AVM, Hubbard, PC. 2010. Olfactory sensitivity to neurotransmitters in goldfish. ECRO - European Chemoreception Research organization XXth Congress, 14-19 September, Avignon, France.

243.Kopecka-Pilarczyk, J, Gonçalves, OM, Coimbra, J, Wilson, JM. 2010. Effects of high hydrostatic selected biomarkers in small-spotted catshark (Scyliorhinus canicula). 9th International Congress on the Biology of Fishes, 5-9 July, Barcelona, Spain.

244.Lahrouni, M, El Khallouf, F, El Ghazali, I, Oufdou, Kh, Vasconcelos, V, Oudra, B. 2010. Effects of cyanotoxins (MC-LR) on the germination, growth and physiology of Vicia faba bean. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

245.Laranjeira, A, Serradeiro, R, Barroso, C, Carvalho, AP, Pacheco, M. 2010. Effect of density and photoperiod on growth and feed utilization of juvenile turbot (Scophthalmus maximus) under intensive production conditions. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

246.Larsen, K, Froufe, E. 2010. Identification of polymorphic species within groups of morphologically conservative taxa: combining morphological and molecular techniques. Congress on Biodiversity Education and Divulgation, Tools for Identifying Biodiversity: Progress and Problems, UNESCO / EDIT / STERNA / KeyToNature, 20-22 September, Paris, France.

247.Leão, P, Pereira, AR, Liu, W-T., Ng, J, Pevzner, P, Dorrestein, PC, König, GM, Vasconcelos, VM, Gerwick, WH.

2010. Molecular techniques for the toxicological assessment of cyanobacterial

blooms in Tunisian freshwaters. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

248.Leão, P, Ramos,

V, Vale, V, Vasconcelos, V. 2010. Response of a Microcystis-dominated planktonic

community to cyanobacterial allelochemicals. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey. (Oral communication)

249.Leitão, F, Ben-Hamadou, R, Chicharo, A, Manjua, A, Morales, J, Moreno, O, Jimenez, C, Malta, Erik-Jan, Sempere, F, Chicharo, L. 2010. DIMEAGUA (Development and Harmonization of commun indicators, metodologies and strategies for the application of the WFD to Guadiana Transitional and Coastal areas). ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. Book of abstracts, p.125.

250.Leite, RB, Cancela, ML. 2010. Changes at transcriptome level during host- parasite interaction using Perkinsus olseni as a model. 35th FEBS Congress Molecules of Life, 26 June 26-1 July, Gothenburg, Sweden. FEBS Journal 277: 118. (Oral communication)

251.León-Cisneros, K, Nogueira, EM, Neto, AI. 2010. Development of Botryocladia macaronesica (Rhodymeniales, Rhodophyta) under laboratory conditions. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.70.


309

252.León-Cisneros, K, Nogueira, EM, Riosmena-Rodriguez, R, Neto, AI. 2010. Life-cycle of Scinaia interrupta (Nemaliales; Rhodophyta). XX International Seaweed Symposium, 22-26 February, Ensenada, México. Book of abstracts, p.74. (Oral communication)

253.Lobo-da-Cunha, A, Oliveira, E, Alves, A, Malheiro, AR, Miranda, L, Resende, AD, Coelho, R, Calado, G. 2010. Comparative analysis of the digestive systems in Bulla striata (Cephalaspidea) and Aplysia depilans (Anaspidea). 3rd International Workshop on Opisthobranchs, 1-4 September, Vigo, Spain (Oral communication)

254.Lobo-da-Cunha, A, Oliveira, E, Alves, A, Santos, T, Sousa, J, Coelho, R, Calado, C. 2010. Comparative study of the crop in the carnivorous opisthobranchs Philinopsis depicta and Aglaja tricolorata (Cephalaspidea, Aglajidae). 17th World Congress of Malacology, 18-24 July, Phuket, Thailand. (Oral communication)

255.Lopes, C, Mix, AC. 2010. Anomalous freshwater inputs in the NE Pacific: possible implications for paleoceanographic reconstructions. 2010 Ocean Sciences Meeting, 22-26 February, Portland, USA.

256.Lopes, C, Mix, AC. 2010. Assessing Paleoceanographic Reconstruction Uncertainties caused by No-Analogs: a NE Pacific Example. American Geophysical Union - AGU Fall Meeting, 5-9 December, San Francisco, California, USA.

257.Lopes, V, Vasconcelos, V. 2010. How different are estuarine cyanobacteria from their freshwater and marine congeners? 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey. (Oral communication)

258.Lopes, VR, Martins, A, Ramos, V, Lobo, V, Welker, M, Vasconcelos, V. 2010. Molecular and chemical trace of cyanobacteria on estuaries. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

259.Lopes, VR, Ramos, V, Martins, A, Vasconcelos, V. 2010. Genotypic and phenotypic profiles of

cyanobacteria from estuaries: are they related to marine and freshwater counterparts? 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

260.Louro, B, Pinto, PIS, Law, A, Canario, AVM, Fontagné, S, Kuhl, H, Matsumura, H, Terauchi, R, Reinhardt, R, De Koning, DJ, Power, DM. 2010. Molecular markers of trout skeletal development under dietary mineral restriction: SuperSAGE with next generation sequencing. Plant and Animal Genomics XVIII Conference, 8-14 January, San Diego, USA. (Oral communication)

261.Louro, B, Pinto, PIS, Law, A, Canario, AVM, Fontagné, S, Kuhl, H, Matsumura, H, Terauchi, R, Reinhardt, R, De Koning, DJ, Power, DM. 2010. SuperSAGE with next generation sequencing: Gene expression effects of mineral-restricted diets in rainbow trout. 32nd Conference for the International Society for Animal Genetics, 26-30July, Edinburgh, United Kingdom. (Oral communication)

262.Luis, L, Almeida, JR, Gravato, C, Guilhermino, L. 2010. Acute effects of naphthalene on mortality and biomarkers of the common prawn (Palaemon serratus). VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica.

263.Luis, L, Gravato, C, Guilhermino, L. 2010. Effects of pyrene on survival, biomarkers and behaviour of the common prawn (Palaemon serratus). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.


310

264.Luis, L, Gravato, C, Guilhermino, L. 2010. Effects of pyrene on the common prawn (Palaemon serratus): biomarkers and behaviour. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal.

265.Ma, S, Diogo, P, Martínez-Páramo, S, Dinis, MT, Sarasquete, C, Cabrita, E. 2010. Effect of antioxidant supplementation in fish sperm extenders on post-thaw sperm quality and DNA fragmentation. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

266.Macedo, JA, Vicente, J, Lomba, A, Henriques, R, Granja, H, Honrado, J. 2010. Early indicators of coastal dynamics in climate change vulnerability assessment. 1st Anual BioPlant Workshop, 29-30 March, Porto, Portugal.

267.Machado, A, Bordalo, AA. 2010. Analysis of bacterial community composition in acidic well water in Guinea-Bissau (West Africa). The Water Research Conference, 11-14 April, Lisbon, Portugal.

268.Machado, A, Bordalo, AA. 2010. Seasonal occurrence of Vibrio cholerae in acidic well water in Guinea-Bissau (West Africa). The Water Research Conference, 11-14 April, Lisbon, Portugal.

269.Madureira, TV, Couto, C, Barreiro, JC, Cass, QB, Pinto, I, Rocha, MJ, Almeida, AA, Tiritan, ME. 2010. Evaluation of the influence of WWTPs on estuarine water quality - correlation between a sewage molecular marker and the metal content on surface waters of the Douro estuary (Portugal). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

270.Magalhães, C, Kiene, RP, Buchan, A, Machado, A, Teixeira, C, Wiebe, WJ, Bordalo, AA. 2010. A novel inhibitory interaction between dimethylsulfoniopropionate (DMSP) and the denitrification pathway. 5th International Symposium on Biological and Environmental Chemistry of DMS(P) and Related Compounds, 19-22 October, Goa, India. (Oral communication)

271.Magalhães, C, Salgado, C, Kiene, RP, Bordalo, AA. 2010. Effect of salinity on microbial DMSP decomposition and DMS formation in estuarine systems. 5th International Symposium on Biological and Environmental Chemistry of DMS(P) and Related Compounds, 19-22 October, Goa, India.

272.Marcos, R, Malhão, F, Galante, H, Monteiro, RAF, Rocha, E. 2010. Influence of age and sex in the number and ultrastructure of the rat hepatic stellate cells: preliminary data. 15th International Symposium on Cells of the Hepatic Sinusoid, 29 August-1 September, Pasadena, California, USA.

273.Martínez, B, Rubal, M, Arenas, F, García-Plazaola, I, Esteban, R, López Figueroa, F, Pereira, R, Sousa-Pinto, I, Burgués, S, Trilla, A, Saldaña, L, Viejo, R. 2010. Fucus serratus response to the warming and tidal stress at its southern rear edge. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain. (Oral communication)

274.Martínez-Páramo, S, Diogo, P, Dinis, MT, Herráez, MP, Cabrita, E. 2010. Does antioxidant addition to the extender produce any effect on European seabass sperm cryoresistance? 47th Annual meeting of the Society for Cryobiology, 17-20 July, Bristol, United Kingdom.

275.Martínez-Páramo, S, Diogo, P, Dinis, MT, Pousão-Ferreira, P, Herráez, MP, Cabrita, E. 2010. Seasonal determination of European seabass (Dicentrarchus labrax) sperm quality: is there any correlation with sperm resistance to cryopreservation? EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.


311

276.Martins, A, Guimarães, L, Soares, AMVM, Guilhermino, L. 2010. Combined effects of temperature and florfenicol on life history traits of the cladoceran Daphnia magna Straus. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

277.Martins, CIM, Castanheira, MF, Engrola, S, Costas, B, Conceição, LEC. 2010. Individual differences in metabolism are predictive of coping styles in Senegalese sole Solea senegalensis. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. (Oral communication)

278.Martins, R, Seabra, R, Ramos, V, Lopes, V, Melo, P, Fernandes, H, Tamagnini, P, Vasconcelos, V. 2010. Evaluation of marine Cyanobium strains for citotoxicity, mutagenicity, genotoxicity and embryogenesis toxicity. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

279.Matos, E, Colen, R, Dinis, MT, Dias, J, Nunes, ML, Bandarra, N, Gonçalves, A. 2010. Effect of dietary plant protein and vegetable oil sources on post-mortem changes in gilthead seabream muscle and its implications on muscle texture. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

280.Matos, E, Dinis, MT, Rodrigues, P, Valente, LMP, Gonçalves, A, Nunes, ML, Dias, J. 2010. Farming practices of gilthead seabream (Sparus aurata) in semi-intensive earth ponds: effects on the flesh quality. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira, Portugal. (Oral communication)

281.Mendes, AC, Martins, D, Castanho, S, Coutinho, J, Bandarra, N, Conceição, LEC, Morais, S, Pousão-Ferreira, P. 2010. Growth and survival of Senegalese sole, Solea senegalensis, fed with different levels of EFA. International Workshop on Sustainable Extensive and Semi-intensive Coast Aquaculture in Southern Europe, 20-21

January, Tavira, Portugal.

282.Mendonça, R, Leitão, A, Barata, EN. 2010. Opercular frequency ventilation reflects the activity and personality of Mozambique tilapia males (Oreochromis mossambicus, Peters 1852). 13th International Behavioral Ecology Conference, 27 September-1 October, Perth, Australia.

283.Mesquita, S, Martins, A, Duarte, D, Gravato, C, Guilhermino, L, Guimarães, L. 2010. Biomarker responses in the shore crab, Carcinus maenas, from two NW Portuguese estuaries, both similar in nature but different in anthropogenic contamination. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

284.Mesquita, SR, Gravato, C, Guilhermino, L, Guimarães, L. 2010. Neurotransmission, energy metabolism and moult-related biomarkers in the shore crab, Carcinus maenas, following cadmium exposure. ICES Annual Science Conference, 20-24 September, Nantes, France. Book of abstracts, p.123.

285.Mesquita, SR, Guilhermino, L, Guimarães, L. 2010. Assessing the effects of the selective serotonin reuptake inhibitor fluoxetine on Carcinus maenas using locomotor behaviour and biomarkers as effect criteria. 27th ESCPBnew Congress - New European Society for Comparative Physiology and Biochemistry, 5-9 September, Alessandria, Italy. (Oral communication)

286.Mesquita, SR, Guilhermino, L, Guimarães, L. 2010. N-acetyl-[beta]-glucosaminidase activity of Carcinus maenas as a possible environmental biomarker for use in ecotoxicological studies. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. Book of abstracts, pp. 140-141.


312

287.Milanin, T, Eiras, JC, Arana, S, Maia, AAM, Silva, MRM, Cariero, MM, Ceccarelli, PS, Adriano, EA. 2010. Análises molecular, histopatológica, ultraestrutural e da prevalência de Myxobolus sp. parasita de Brycon hilarii (Vharacidae), do Pantanal Mato-Grossense. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil.

288.Mil-Homens, M, Costa, A, Fonseca, S, Serrano, R, Mateus, M, Melo, Z, Sousa, R, Trancoso, A. 2010. Concentrações naturais de metais em traço em sedimentos do Estuário do Rio Minho: Valores de Baseline para estudos ambientais. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal.

289.Mira, S, Madeira, C, Cabrita, E, Soares, F, Cancela, ML, Dinis, MT. 2010. Parental contribution of Solea senegalensis assessed with microsatellite markers. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

290.Mohseni, M, Ozório, ROA, Pourali, H, Lee, S, Bai, SC. 2010. Optimal dietary lipid to carbohydrate ratio in beluga sturgeon (Huso huso) fed different L-carnitine levels. Aquaculture Europe 2010, October 5-8, 2010, Porto, Portugal.

291.Monteiro, P, Bentes, L, Gonçalves, JMS. 2010. Collating habitat maps. Current situation – Portugal. Workshop on Collating Habitat Maps, MESH Atlantic, 18-21 October, Brest, Portugal. (Oral communication)

292.Monteiro, P, Veiga, P, Oliveira, F, Bentes, L, Oliveira, MO, Afonso, C, Erzini, K, Gonçalves, JMS. 2010. Habitats classification of the South coast of Portugal (Algarve) using the EUNIS (European Nature Information System) approach. International Coastal Conservation Conference - ICCCM, 12-17 April, Estoril, Portugal.

293.Monteiro, P, Veiga, P, Oliveira, F, Bentes, L, Rangel, MO, Afonso, C, Sousa, I, Leite, L, Erzini, K, Gonçalves, JMS. 2010. Assessment of macro-scale habitat preferences of two comber species (Serranus hepatus and Serranus cabrilla) off the south coast of Portugal (Algarve). XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain.

294.Moraes, JRE, Bozzo, FR, Ozório, ROA, Moraes, FR. 2010. Hematological parameters in pacu (Piaractus mesopotamicus) after swim bladder injection of thioglycolate, lipopolysaccharide and inactivated Aeromonas hydrophila. 9th International Congress on the Biology of Fish, 5-9 July, 2010, Barcelona, Spain.

295.Moraes, JRE, Moraes, FR, Sucasas, LF, Peres, H, Oliva-Teles, A, Ozório, ROA. 2010. Influence of dietary vitamin e and l-carnitine on the wound healing process in European sea bass. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

296.Morais, P de, Stoichev, T, Basto, MCP, Vasconcelos, MT. 2010. Optimization of solid-phase microextraction coupled to gas chromatography (SPME-GC-ECD) for determination of chlorophenols in water samples. 12th International Symposium on Advances in Extraction Technologies - ExTech 2010, 20 - 22 September, Poznan, Poland. Book of Abstracts, p.107.

297.Moreira, C, Martins, A, Vasconcelos, V, Antunes, A. 2010. Variação e dinâmica da comunidade de cianobactérias no estuário do Rio Minho. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

298.Moreira, C, Vasconcelos, V, Antunes, A. 2010. Evolution and phylogenetics of worldwide strains of the invasive cyanobacterium Cylindrospermopsis raciborskii. SMBE 2010 - Annual Meeting of the Society for Molecular Biology and Evolution, 4-8 July, Lyon, France.


313

299.Moreira, C, Vasconcelos, V, Antunes, A. 2010. Phylogeny of microcystins produced by Microcystis aeruginosa: evidences for a biogeographical trend?. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

300.Mota, M, Antunes, C, Bio, A, Costa-Dias, S, Sousa, R. 2010. Temporal changes in the ichthyofauna at a tidal freshwater zone of the River Minho. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

301.Mucha, AP, Almeida, CMR, Magalhães, C, Reis, I, Guedes, P, Almeida, R, Couto, MN, Bordalo, AA. 2010. Microbial community response to the Prestige buried fuel in intertidal beach zones. The Water Research Conference, 11-14 April, Lisbon, Portugal.

302.Mucha, AP, Almeida, CMR, Magalhães, CM, Reis, I, Guedes, P, Almeida, R, Bordalo, AA. 2010. Contribution of the microbial community for the degradation of buried fuel in the intertidal beach zone. The Water Research Conference, 11-14 April, Lisbon, Portugal.

303.Naughton, F, Sánchez Goñi, MF, Duprat, J, Cortijo, E, Abrantes, F. 2010. Climate variability during the last deglaciation in north-western Iberian margin and adjacent landmasses. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal. (Oral communication)

304.Navarro, G, Flecha, S, Prieto, L, Olivé, I, Taglialatela, S, Rouco, M, Roque, D, Moreno, A, Ferrer-Marco, M, Relvas, P, Ruiz, J, Huertas, IE. 2010. Masas de agua y nutrientes inorgánicos en el Golfo de Cádiz: distribución y análisis. XV Iberan Seminar of Marine Chemistry, 22-24 February, Vigo, Spain. (Oral communication)

305.Nave, S, Lebreiro, S, Kissel, C, Guihou, A, Figueiredo, MO, Silva, TP, Michel, E, Cortijo, E, Labeyrie, L, Voelker, A. 2010. Open oceanic productivity changes at mid-latitudes during interglacials and its relation to the Atlantic Meridional Overturning Circulation. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-14696-1. (Oral communication)

306.Nave, S, Lebreiro, S, Kissel, C, Guihou, A, Figueiredo, MO, Silva, TP, Michel, E, Cortijo, E, Labeyrie, L, Voelker, A. 2010. Oceanic biological productivity changes off Iberia during MIS 5 and its relation to the Atlantic Meridional Overturning Circulation. 10th International Conference on Paleoceanography, 29 August-3 September, La Jolla, San Diego, USA.

307.Neto, AI, Martins, GM, Wallenstein, FM, Titley, I. 2010. Phycological research in the Azores: past, present and future. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.33. (Oral communication)

308.Neto, AI, Patarra, RF, Brotas, V, Álvaro, NV, Gameiro, C, Silva, A, Diniz, T, Prestes, ACL, Azevedo, JMN, Medeiros, MC, Pacheco, DM, Gaspar, JL. 2010. Ecological quality of Azorean coastal waters. International Conference on Coastal Conservation and Management in the Atlantic and Mediterranean, 12-17 April, Estoril, Portugal. Book of abstracts, p.16. (Oral communication)

309.Nogueira, EM, Medeiros, J, Neto, AI. 2010. Algal collections in the Herbarium Ruy Telles Palhinha, AZB. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.40.

310.Nogueira, M, Teixeira, AT, Varandas, S, Lopes-Lima,

M, Hinzmann, M, Machado, J, Cortes, RM.

2010. Caracterização ecológica das populações de mexilhão-de-rio (Margaritifera margaritifera L.) e de truta (Salmo trutta L.) na bacia hidrográfica do Rio Tua (NE Portugal). XV Iberian Congress of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal.


314

311.Novais, S, Gomes, S, Gravato, C, Guilhermino, L, Soares, AMVM, De Coen, W, Amorim, MJB. 2010. Reproduction and biochemical responses in Enchytraeus albidus (Oligochaeta) exposed to heavy-metals: effects of zinc and cadmium. VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

312.Novais, S, Howcroft-Ferreira, C, Gravato, C, Guilhermino, L, De Coen, W, Soares, AMVM, Amorim, MJB. 2010. From gene to population - linking effects from different levels of organization in Enchytraeus albidus - microarrays, biomarkers and traditional toxicity tests. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain. (Oral communication)

313.Oliveira, C, Dinis, MT, Mañanos, E, Sánchez-Vázquez, J. 2010. Current knowledge on reproduction rhythms in Senegal sole, Solea senegalensis. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

314.Oliveira, C, Dinis, MT, Mañanos, E, Sánchez-Vázquez, J. 2010. Reproduction rhythms in Senegal sole: synchronization to seasonal, lunar and daily cycles. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain.

315.Oliveira, C, Gravato, C, Soares, AMVM, Guilhermino, L. 2010. Acute effects of fenitrothion on Palaemon serratus assessed at different levels of biological organization. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain. (Oral communication)

316.Oliveira, DV, Cavaco, SI, Cordeiro, OD, Silva, TS, Rodrigues, PM, Simes, DC. 2010. Proteomic analysis of nacre soluble matrix proteins from Crassostrea gigas. 4th EuPA Meeting and 6th ProCura Meeting, 23-27 October, Estoril, Portugal.

317.Oliveira, M, Vieira, LR, Gravato, C, Guilhermino, L. 2010. Acute effects of pyrene on the common goby Pomatoschistus microps (Teleostei, Gobiidae). XII International Congress of Toxicology – IUTOX 2010, 19-23 July, Barcelona, Spain.

318.Oliveira, M, Vieira, LR, Gravato, C, Guilhermino, L. 2010. Pyrene effects on Pomatoschistus microps (Teleostei, Gobiidae) - An acute study. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal.

319.Oliveira, P, Gravato, C, Guilhermino, L. 2010. Relationships between polycyclic aromatic hydrocarbons (PAHs) "metabolites" (2,3,4 and 5 rings) levels and biomarker responses in wild populations of mussels from the portuguese NW coast. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

320.Oliveira, RF, Balteiro, J, Alves, MJ, Coelho, C, Martins, R, Pereira, MJ, Cruz, A. 2010. Avaliação, in vitro, da sensibilidade Giardia lamblia ao metronidazol recorrendo a diferentes metodologias. XX Pan-American Congress of Pharmaceutics and XIV Congress of the South American Pharmaceutical Federation, 25-29 May, Porto Alegre, Brazil.

321.Oropesa, AL, Soler, F, Martins, A, Guimarães, L, Guilhermino, L. 2010. Acute and chronic toxicity of the progestin levonorgestrel to Daphnia magna. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

322.Osswald, J, Passo, J, Vasconcelos, V, Guilhermino, L. 2010. Determinação do factor de bioacumulação da anatoxina-a na truta arco-íris (Oncorhincus mykiss), por HPLC-FL, em


315

condições laboratoriais. XV Iberian Congress of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. (Oral communication)

323.Ozorio, ROA, Hubbard, PC, Barata, EN, Valente, LMP, Canario, AMV. 2010. Olfactory sensitivity to amino acids in the blackspot seabream (Pagellus bogaraveo Brunnich 1768): how effective is the electro-olfactogram in seawater? EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. Book of abstracts, pp. 946-947.

324.Ozorio, ROA, Peres, H, Valente, LMP, Oliva-Teles, A, Gonçalves, J. 2010. Attractant and antioxidant roles of dietary supplemental levels of selected amino acids in blackspot seabream (Pagellus bogaraveo Brunnich 1768). EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

325.Pacchiarini, T, Cabrita, E, Cross, I, Ortiz-Delgado, JB, Pousão-Ferreira, P, Gavaia, PJ, Rebordinos, L, Sarasquete, C. 2010. The vasa maternal factor in Senegalese sole, Solea senegalensis: molecular characterization and gene expression pattern analysis during early development and adulthood. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

326.Palma, J, Bureau, DP, Andrade, JP. 2010. Effect of broodstock nutrition on the long snout seahorse (Hippocampus guttulatus) reproduction and brood quality. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

327.Palma, J, Bureau, DP, Andrade, JP. 2010. Optimizing Artemia enrichment and feeding protocol when rearing juvenile long snout seahorse, Hippocampus guttulatus. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

328.Palma, J, Bureau, DP, Andrade, JP. 2010. Using enriched shrimp as a diet for long snout seahorse, Hippocampus guttulatus. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

329.Palumbo, E, Amore, FO, Flores, J-A, Volker, A. 2010. Paleo-climate changes during Termination V off Iberia as revealed by coccoliths assemblages. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-6388-2. (Oral communication)

330.Páscoa, I, Fontaínhas-Fernandes, A, Castro, LFC, Wilson, J.M. 2010. Effects of prolactin and cortisol on branchial ammonia transporter expression in zebrafish. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.

331.Patarra, RF, Brotas, V, Álvaro, NV, Gameiro, C, Silva, A, Diniz, T, Prestes, ACL, Azevedo, JMN, Medeiros, MC, Pacheco, DM, Gaspar, JL, Neto, AI. 2010. Ecological quality of Azorean coastal waters: Macrophytes and Phytoplankton evaluation. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.53. (Oral communication)

332.Patarra, RF, Paiva, L, Leite, J, Lima, E, Neto, AI, Baptista, J. 2010. Nutritional Value and Antioxidant Activity of Selected Azorean Macrophytes. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal.

333.Patton, G, Martin, PA, Voelker, A. 2010. Trace element/Ca measured in planktonic foraminifera during Heinrich Events in the Iberian Margin and Gulf of Cadiz. 10th International Conference on Paleoceanography, 29 August-3 September, La Jolla, San Diego, USA.


316

334.Pearson, GA, Lago-Leston, A, Canovas, F, Agusti, S, Duarte, C, Serrão, EA. 2010. Exploring phytoplankton communities in the Southern Ocean using metatranscriptomics. ESF-COST High-Level Research Conference, Marine Biotechnology: Future Challenges, 20-25 June, Acquafredda di Maratea, Italy.

335.Pearson, GA, Serrão, EA, Canovas, F, Lago-Leston, A, Neiva, J, Nicastro, K, Zardi, G. 2010. Environmnental genomics of Fucus: stress management, mergers and acquisitions in the intertidal zone. Ectocarpus 2010 - a Decade of Algal Genomics, 7-9 April, Ghent, Belgium. (Invited oral communication)

336.Peleteiro, JB, Rodríguez-Villanueva, JL, Escorcio, C, Alvárez-Blázquez, B, Bacelar, M, Linares, F,

Saavedra, MJ, Cal, RM, Magadán, S, Valente, LMP. 2010. Is recirculation the system for Senegal sole Solea senegalensis cultura? Comparison of two cultura systems using a control diet and a plant-based diet until market size. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. Book of abstracts, pp. 986-987.

337.Pereira, H, Barreira, L, Custódio, L, Varela, J. 2010. Optimization of lipid production in marine microalgal species by biochemical engineering and high throughput screening. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal.

338.Pereira, H, Sobral, AF, Custódio, L, Barreira, L, Varela, J. 2010. Novel microalgal strains from the Algarve coast for biofuel production. 8th European Workshop of Microalgal Biotechnology, 7-10 June, Nuthetal, Germany.

339.Pereira, MJR, Palming, J, Svensson, MK, Aureliano, M, Carvalho, E, Eriksson, JW. 2010. Effects of immunosuppressive agents on glucose and lipid metabolism in human subcutaneous adipocytes. American Diabetes Association's 70th Scientific Sessions, 25-29 June, Orlando, Florida, USA.

340.Pereira, MJR, Palming, J, Svensson, MK, Aureliano, M, Carvalho, E, Eriksson, JW. 2010. Immunosuppressive agents alter insulin signalling and glucose and lipid metabolims in human subcutaneous adipocytes. 46th Annual Meeting of the European Association for the Study of Diabetes, 20-24 September, Stockholm, Sweden.

341.Pereira, MJR, Palming, J, Svensson, MK, Aureliano, M, Carvalho, E, Eriksson, JW. 2010. Effects of immunosuppressive agents on glucose and lipid metabolism in human subcutaneous adipocytes. American Diabetes Association's 70th Scientific Sessions, 25-29 June, Orlando, Florida, USA. (Oral communication)

342.Pereira, R, Abreu, H, Valente, L, Rema, P, Sousa-Pinto, I. 2010. Production of seaweeds in integrated multitrophic aquaculture for application as ingredients in fish-feed. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. (Oral communication)

343.Pereira, R, Valente, L, Sousa-Pinto, I, Rema, P. 2010. Digestibility coeficients of IMTA produced seaweeds, by rainbow trout (Oncorhynchus mykiss) and nile tilapia (Oreochromis niloticus). 49th Annual Northeast Algal Symposium, 16-18 April, Bristol, USA.

344.Pereira, R, Valente, L, Sousa-Pinto, I, Rema, P. 2010. Apparent digestibility coeficients of fours species of seaweeds by rainbow trout (Oncorhynchus mykiss) and nile tilapia (Oreochromis niloticus). Aquaculture 2010, Meeting of the World Aquaculture Society, 1-5 March, San Diego, USA.

345.Pereira, R, Valente, L, Sousa-Pinto, I, Rema, P. 2010. Evaluation of IMTA produced seaweeds Gracilaria, Porphyra and Ulva as dietary ingredients in Nile tilapia (Oreochromis niloticus). EAS


317

Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. Book of abstracts, pp. 994-995. (Oral communication)

346.Pereira, T, Varó, I, Vieira, MN, Torreblanca, A. 2010. Effects of temperature change on three biomarkers in gilthead seabram (Sparus aurata). 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

347.Peres, H, Perez-Jimenez, A, Rubio, V, Oliva-Teles, A. 2010. Role of white tea and methionine supplements on growth, body composition and oxidative stress defences of gilthead sea bream (Sparus aurata) juveniles. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

348.Peres, H, Santos, S, Oliva-Teles, A. 2010. Selecetd plasma biochemistry parameters in fed and unfed of gilthead seabream (Sparus aurata) and European seabass (Dicentrarchus labrax). EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

349.Perez-Jimenez, A, Peres, H, Oliva-Teles, A. 2010. Effect of hypoxia and feed composition on antioxidant enzymatic defenses of Sparus aurata fingerlings. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

350.Pinheiro, C, Azevedo, J, Campos, A, Loureiro,

L, Vasconcelos, V. 2010. Effects of cyanobacterial

extracts containing the toxins microcystin-LR and cylindrospermopsin on the growth of microalgae. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

351.Pinto, W, Figueira, L, Conceição, L, Dinis, MT, Aragão, C. 2010. Lipossome enrichment enables taurine delivery to Sparus aurata larvae. XVIII International Conference on Bioencapsulation, 1-2 October, Porto, Portugal.

352.Pinto, W, Figueira, L, Rodrigues, V, Dinis, MT, Aragão, C. 2010. Aromatic amino acid metabolism mirrors fish metamorphosis complexity. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. (Oral communication)

353.Pinto, W, Rønnestad, I, Jordal, AE, Dinis, MT, Aragão, C. 2010. Expression and fuctionality of taurine transporter (TauT) in Senegalese sole (Solea senegalensis) larvae and juveniles. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal. (Oral communication)

354.Pinto, W, Santos, A, Dinis, MT, Aragão, C. 2010. Dietary taurine supplementation increases methionine retention in gilthead seabream (Sparus aurata) larvae. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain.

355.Pintor, S, Fidalgo, ML. 2010. Toxicidade aguda do herbicida alacloro face ao peixe zebra Danio rerio em estádios iniciais do seu ciclo de vida. XV Iberian Congress of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. (Oral communication)

356.Portella, MC, Mai, MG, Engrola, S, Morais, S, Verani, JR, Dinis, MT, Conceicão, LEC. 2010. Co-feeding of live feed and inert diet from first-feeding affects Artemia lipid digestibility and retention in Senegalese sole (Solea senegalensis) larvae. Aquaculture 2010, Meeting of the World Aquaculture Society, 1-5 March, San Diego, USA. (Oral communication)

357.Power, DM, Louro, B, Pinto, PIS, Law, A, Fontagné, S, Kuhl, H, Matsumura, H, Terauchi, R, Reinhardt, R, De Koning, DJ, Canario, AVM. 2010. The global transcriptome during delayed


318

skeletogenesis induced by mineral restriction in rainbow trout. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Barcelona, Spain. (Oral communication)

358.Prabhu, CN, Vinaychandran, PN, Vidal, L, Nave, S, Abreu, L, Abrantes, F. 2010. Variations in northern Indian Ocean dynamics and primary production during the late Quaternary. 13th International Nannoplankton Association Meeting, 5-10 September, Yamagata, Japan.

359.Prieto, A, Puerto, M, Campos, A, Cameán, A, Vasconcelos, V. 2010. Activity of glutathione S-transferase in Mytilus galloprovincialis and Corbicula fluminea exposed to toxic Cylindrospermopsis raciborskii cells. XII International Congress of Toxicology -IUTOX 2010, 19-23 July, Barcelona, Spain.

360.Prieto, A, Puerto, M, Campos, A, Cameán, A, Vasconcelos, V. 2010. Analysis of glutathione S-transferase and glutathione peroxidase in the rice plant (Oryza sativa L.) exposed to Aphanizomenon ovalisporum and Microcystis aeruginosa cell extracts. XII International Congress of Toxicology -IUTOX 2010, 19-23 July, Barcelona, Spain.

361.Puerto, MP, Prieto, AI, Campos, AM, Cameán, AM, Vasconcelos, VM. 2010. Differential protein expression in Mytilus galloprovincialis and Corbicula fluminea exposed to Cylindrospermopsis raciborskii cyanobacteria cells. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

362.Queiroz, CA, Kijjoa, A, David, JM, David, JP. 2010. Flavonoids and benzoic acid derivatives from Dioclea virgata. 58th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research, 29 August-2 September, Berlin, Germany.

363.Quintã, R, Santos, R, Thomas, D, Le Vay, L. 2010. Evaluation of Salicornia europaea as a biofilter in intensive mariculture: plant growth and nitrogen uptake under different nitrogen regimes. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

364.Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2010. Fish as a suitable model to unveil the function of the four and half LIM domains protein 2. 35th FEBS Congress Molecules of Life, 26 June -1 July, Gothenburg, Sweden. FEBS Journal 277: 183.

365.Ramalho, A, Conceição, LEC, Dias, J, Vaz-Pires, P, Valente, L, Cunha, ME, Yúfera, M, Hussenot, J, Cardinal, M, Blanchier, P, Anras, L, Bailly, D, Raux, P, Marino, G, Boglione, C, Patarnello, P, Makridis, M, Kentouri, M, Dinis, MT. 2010. Sustainable extensive and semi-intensive coastal aquaculture in Southern Europe: SEACASE project – an overview. Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1476-1477.

366.Ramos, S, Amorim, E, Elliott, M, Cabral, HN, Bordalo, AA. 2010. Early life stages of fishes as indicators of estuarine ecosystem condition. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal.

367.Ramos, S, Moura, JJG, Aureliano, M. 2010. Actin as a potential target for vanadyl: a possible explanation for many vanadium biological effects. 10th European Biological Inorganic Chemistry Conference, 22-26 June, Tessalonica, Greece.

368.Ramos, S, Moura, JJG, Aureliano, M. 2010. Decavanadate effects in actin structure and function. 35th FEBS Congress, 26 June-1 July, Gothenburg, Sweden.


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369.Ramos-Pereira, A, Ramos, C, Trindade, J, Araújo-Gomes, J, Rocha, J, Granja, H, Gonçalves, J, Monge-Soares, A, Martins, J. 2010. Presentation of the FMI 5000 Project: Environmental changes: Fluvio-marine interactions over the last 5000yrs. EGU General Assembly, 3-7 May, Viena, Austria.

370.Range, P, Chícharo, L, Chícharo, MA, Ben-Hamadou, R, Piló, D, Matias, D, Joaquim, S, Oliveira, AP. 2010. Effects of ocean acidification on juvenile clams Ruditapes decussatus. 39th CIESM Congress, 10-14 May, Venice, Italy. Book of abstracts, p.789. (Oral communication)

371.Rangel, MO, Gonçalves, JMS, Abreu, F, Costa, C, Erzini, K. 2010. Implementation of ecotourist underwater snorkeling routes in the Marinha Beach (Algarve) as a way of preserving. International Coastal Conservation Conference - ICCCM, 12-17 April, Estoril, Portugal. (Oral communication)

372.Rangel, MO, Gonçalves, JMS, Costa, C, Erzini, K. 2010. Managing underwater eco-tourism impacts in Algarve coastal area (South Portugal). XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain.

373.Rangel, MO, Gonçalves, JMS, Costa, C, Leite, L, Erzini, K. 2010. Implementation of eco-tourist underwater routes in the Algarve (South of Portugal) a way of preserving exuberant marine ecosystems. INVTUR 2010, 10-13 March, Aveiro, Portugal. (Oral communication)

374.Rangel, MO, Gonçalves, JMS, Leite, L, Costa, C, Erzini, K. 2010.Eco-tourism underwater snorkelling routes in the Marinha Beach (Algarve). A way of preserving pristine marine ecosystems 3rd International Conference on the Management of Coastal Recreational Resources, 27-30 October, Grosseto, Italy.

375.Rebelo, H, Froufe, E, Ferrand, N, Jones, G. 2010.Where is my bat? Ground-validation of presence-only modelling and its integration with molecular techniques for the conservation of rare species. XVth International Bat Research Symposium, 22-27 August, Prague, Czech Republic.

376.Rebotim, A, Voelker, AH, Waniek, J. 2010. Planktonic foraminifera as indicators of water masses north and south of the Azores Front/Current: Evidence from abundance and stable isotopes data. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal.

377.Rebotim, A, Voelker, AH, Waniek, J. 2010. Planktonic foraminifera as indicators of water masses north and south of the Azores Front/Current: Evidence from abundance and stable isotopes data. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-4940.

378.Rebotim, A, Voelker, AH, Waniek, J. 2010. Planktonic foraminifera as indicators of water masses north and south of the Azores Front/Current: Evidence from abundance and stable isotopes data. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany.

379.Rebotim, A. 2010. Planktonic foraminifera as indicators of water masses north and south of the Azores Front/Current: Evidence from abundance and stable isotopes data. ECORD Summer School 2010 – Dynamics of Past Climate Changes, 13-24 September, Bremen, Germany. (Oral communication)

380.Reis, B, Urbatzka, R, Cruzeiro, C, Ferreira, C, Monteiro, RAF, Rocha, E, Rocha, MJ. 2010. Evaluation of steroidogenic and morphological impacts in adult zebrafish (Danio rerio) exposed to environmental levels of xenoestrogens found in Douro River estuary, Portugal. 20th Annual


320

Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

381.Reis-Henriques, MA, Laudet, V, Schubert, M, Santos, MM. 2010. Ancestral bilaterian retinoic acid signaling: characterization of the Retinoic Acid Receptor (RAR) from the lophotrochozoan mollusc Nucella lapillus. CECE 25 - 25th Conference of the European Comparative Endocrinologists, 31 August-4 September, Pécs, Hungary. (Oral communication)

382.Relvas, P, Santos, AMP, Luís, J, Laginha Silva, P. 2010. Consequences of the global warming in the structure of the Iberian Atlantic coastal ocean. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal (Oral communication)

383.Rema, P, Pedrosa, K, Grassi, G, Dias, J. 2010. Dietary hempseed meal and hempseed oil enhanced growth performance in juvenile turbot (Schophthalmus maximus). 14th International Symposium on Fish Nutrition and Feeding, 31 May-4 June, Qingdao, China.

384.Renfro, JL, Guerreiro, PM, Bataille, AM. 2010. Inorganic phosphate (Pi) is actively transported from cerebrospinal fluid (CSF) by the choroid plexus (CP). American Physiological Society - Experimental Biology Meeting, 24-28 April, Anaheim, CA, USA. FASEB Journal 24 (Suppl.): 813.9.

385.Ribeiro, C, Urbatzka, R, Castro, F, Carrola, J, Fernandes, AF, Monteiro, RAF, Rocha, E, Rocha, MJ. 2010. In vitro exposure of Nile tilapia (Oreochromis niloticus) testis to environmental concentrations of estrogenic disrupting compounds induces upregulation of some genes encoding steroidogenic enzymes. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

386.Ribeiro, M, Figueiredo, F, Cruzeiro, C, Rocha, E, Rocha, MJ. 2010. Chemical monitoring of several classes of pesticides reveals high sediment pollution load in the Douro River estuary, Porto, Portugal. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

387.Ribeiro, M, Figueiredo, F, Ribeiro, C, Cruzeiro, C, Rocha, E, Rocha, MJ. 2010. Evaluation of dissolved oxygen and of estrogenic endocrine disruptor compounds in the waters of the Leça River and Estuary (upstream of the Leixões Harbour), Portugal. 20th Annual Meeting of the Society of Environmental Toxicology and Chemistry – SETAC Europe 2010, 23-27 May, Seville, Spain.

388.Ribeiro, S, Amorim, A, Abrantes, F, Ellegaard, M. 2010. Resolving the historical record of Gymnodinium catenatum and other microreticulate cysts in the NE Atlantic. 14th International Conference on Harmful Algae, 1-5 November, Crete, Greece.

389.Richard, M, Cardinal, M, Fabre, R, Maurice, J-T, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Valente, LMP, Cariu, S, Hamdaoui, M, Hussenot, J. 2010. Improving flesh quality of intensive systems by a short crossing in effluent treatment ponds. International Workshop on Sustainable Extensive and Semi-intensive Coastal Aquaculture in Southern Europe, 20-21 January, Tavira, Portugal. Book of abstracts, p.77.

390.Richard, M, Cardinal, M, Fabre, R, Maurice, J-T, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Valente, LMP, Cariu, S, Hamdaoui, M, Hussenot, J. 2010. Improving the fish quality of an intensive system by a short crossing in algae ponds. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1097-1098.


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391.Richard, N, Cordeiro, O, Silva, TS, de Vareilles, M, Alves, RN, Costas, B, Wulff, T, Rodrigues, PM, Conceição, LEC. 2010. Effects of amino acid supplementation on liver proteome of Senegalese sole juveniles exposed to handling stress. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1101-1102.

392.Richard, N, de Vareilles, M, Gavaia, PJ, Silva, TS, Cordeiro, O, Yúfera, M, Rodrigues, PM, Conceição, LEC. 2010. Inclusion of protein hydrolysates in the diet of white seabream (Diplodus sargus) larvae: impacts on skeleton quality and larvae proteome expression. 14th International Symposium on Fish Nutrition and Feeding, 31 May-4 June, Qingdao, China.

393.Richard, N, Pinto, W, Cordeiro, O, Silva, TS, Conceição, LEC, Rodrigues, PM, Gavaia, PJ. 2010. Impacts of dietary vitamin K supplementation on skeleton quality and proteome expression of Senegalese sole larvae. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1478-1479.

394.Roberto, VP, Tiago, DM, Gautvik, K, Cancela, ML. 2010. Zebrafish as an in vivo and in vitro model to study microRNAs in bone. EMBO/EMBL Symposium - The Non-Coding Genome, 13-16 October, Heidelberg, Germany.

395.Robles, V, Barbosa, V, Martínez-Páramo, S, Herráez, MP, Izpisua-Belmonte, JC. 2010. Cryopreservation of zebrafish embryonic pluripotent cells. 1st International Congress on Controversies in Cryopreservation of Stem Cells, Reproductive Cells, Tissue & Organs, 22-25 April, Valencia, Spain.

396.Rocha, F, Alves Martins, D, Martínez-Rodríguez, G, Castanheira, MF, Morais, S, Coutinho, J, Bandarra, NM, Conceição, LEC. 2010. Dietary arachidonic acid effects on growth performance and fatty acid metabolism in gilthead seabream larvae. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

397.Rodrigues, T, Grimalt, J, Abrantes, F, Naughton, F, Flores, J-A. 2010. Abrupt sea surface temperature changes during the Last Glacial-Interglacial Transition in the Iberian margin: Sea level implications. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-9047.

398.Rodrigues, V, Dias, J, Rema, P, Silva, S. 2010. Mapping body fat distribution in farmed Senegalese sole (Solea senegalensis). EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1120-1121.

399.Rosa, JT, Cancela, ML, Laizé, V. 2010. Role of a short-chain dehydrogenase/reductase in fish skeletogenesis and tissue mineralization. European calcified tissue society PhD Training Course, 9-12 September, Berlin, Germany.

400.Rosas-Alquicira, EF, Hernández-Carmona, G, Riosmena-Rodriguez, R, Neto, AI. 2010. Ontogeny of conceptacles in Amphiroa (Corallinales, Rhodophyta). XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. Book of abstracts, p.96. (Oral communication)

401.Rosas-Alquicira, EF, Riosmena-Rodriguez, R, Neto, AI. 2010. Diagnosing characters in Amphiroa (Corallinales) and the genus diversity evaluation in the Azores. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. Book of abstracts, p.162.

402.Salgueiro, E, de Abreu, L, Voelker, A, Prabhu, CN, Vaqueiro, S, Abrantes, F, Duprat, J, Turon, J-L. 2010. Temperature and productivity gradients within the Western Iberian upwelling system in


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response to glacial and Heinrich-Event climate forcing. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany. (Oral communication)

403.Salgueiro, E, de Abreu, L, Voelker, A, Vaqueiro, S, Abrantes, F, Duprat, J, Turon, J-L. 2010. Temperature and productivity gradients along the Western Iberian Margin during the last 50ky. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal. (Oral communication)

404.Salgueiro, E, Martin, P, Voelker, A, Prabhu, CN, Abrantes, F. 2010. MIS 11c and Holocene temperature on the Portuguese Margin as revealed by Mg/Ca and oxygen stable isotopes. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany.

405.Sánchez Goñi, MF, Michel, E, Desprat, St, Carlson, A, Naughton, F, Fletcher, WJ, Rossignol, L. 2010. European warming linked to Greenland melting during the Last Interglacial North Atlantic climate optimum. American Geophysical Union - AGU Fall Meeting, 5-9 December, San Francisco, California, USA. (Oral communication)

406.Santos, C, Abrantes, F, Rodrigues, T, Voelker, A, Vis, G-J. 2010. Paleoenvironmental and paleoclimatic conditions in theTagus River Estuary during the Holocene. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-14481.

407.Santos, C, Abrantes, F, Rodrigues, T, Voelker, A. 2010. Paleoenvironmental and paleoclimatic conditions in the Tagus River Estuary during the last ~14kyr. ECORD Summer School 2010 – Dynamics of Past Climate Changes; 13-24 September, Bremen, Germany. (Oral communication)

408.Santos, C, Abrantes, F, Rodrigues, T, Voelker, A. 2010. Paleoenvironmental and paleoclimatic conditions in the Tagus River Estuary during the last ~14kyr. II IGBP Iberian Seminar: Global Change in the Iberian Peninsula: An integrated approach, 4-5 November, Lisbon, Portugal

409.Santos, MM, Lima, D, Moreira, SM, Neuparth, T, Reis-Henriques, MA. 2010. Developing monitoring tools for oil and HNS spills. 6th. International Conference on Marine Pollution and Ecotoxicology, 31 May-3 June, Hong Kong, China. (Oral communication)

410.Santos, MM, Solé, M, Lima, D, Hambach, B, Ferreira, AM, Reis-Henriques, MA. 2010. Validating a multi-biomarker approach with the shanny Lipophrys pholis to monitor oil spills in European marine ecosystems. VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

411.Santos, R, Costa, R, Silva, J, Coelho, H, Leitão, JC. 2010. Management of a Sphaerococcus coronopifolius bed that causes considerable economic losses. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. (Oral communication)

412.Santos, R, Mata, L. 2010. Sustainable, integrated semi-intensive aquaculture of seaweeds and fish in southern Europe. International Workshop on Sustainable Extensive and Semi-intensive Coastal Aquaculture in Southern Europe, 20-21 January, Tavira, Portugal.

413.Santos, R. 2010. European seagrass network: Seagrass productivity, from genes to ecosystem management. World Seagrass Biology Conference and International Seagrass Workshop 2010, 22-30 November, Phuket and Trang, Thailand. (Oral communication)

414.Saraiva, A. 2010. Os anisaquídeos e sua importância na Saúde Pública. XI ENBRAPOA, 19-22 July, Campinas, São Paulo, Brazil. (Oral communication)


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415.Seixas, P, Otero, A, Valente, LMP, Rey-Méndez, M. 2010. The hard task of rearing Octopus vulgaris paralarvae. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1219-1220.

416.Serrao, EA, Pearson, GA, Canovas, F, Neiva, J, Nicastro, K, Zardi, Genetic signatures of evolutionary dynamics and range shifts in canopy-forming marine species. Nordic Marine Sciences Conference 2010, 13-16 September, Stromstad, Sweden. (Oral invited communication)

417.Silva, B, Pereira, R, Abreu, MH, Sousa-Pinto, I. 2010. Evaluation of the brown alga Stypocaulon scoparium as a candidate for integrated multi-trophic aquaculture. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico. (Oral communication)

418.Silva, J, Barrote, I, Albano, S, Costa, M, Neves, P, Graça, G, Sharon, Y, Beer, S, Santos, R. 2010. Effects of in situ shading on the photophysiology of Zostera marina and Cymodocea nodosa. World Seagrass Biology Conference and International Seagrass Workshop 2010, 22-30 November, Phuket and Trang, Thailand. (Oral communication)

419.Silva, J. 2010. From seagrass photosynthesis to community metabolism. Interuniversity Institute for Marine Sciences, Eilat, Israel. (Invited oral communication)

420.Silva, JMG, Espe, M, Conceição, LEC, Dias, J, Costas, B, Valente, LMP. 2010. Use of alternative protein sources in diets of Senegalese sole juveniles (Solea senegalensis, Kaup 1858). EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1238-1239. (Oral communication)

421.Silva, SR, Guedes, CM, Loureiro, N, Mena, E, Dias, J, Rema, P. 2010. Prediction in vivo of the fillet volume in senegalese sole (solea senegalensis) by multiple consecutive transverse real time ultrasonography images. Foodsim'2010 - 6th International Conference on Simulation and Modelling in the Food and Bio-industry, 24-26 June, Bragança, Portugal.

422.Silva, TS, Cordeiro, OD, Jessen, F, Dias, J, Rodrigues, PM. 2010. Assessment of the reproducibility of a muscle fractionation procedure for comparative proteomics using 2DE and metric multidimensional scaling. HUPO 9th Annual World Congress, 19-23 September, Sydney, Australia.

423.Silva, TS, Dias, J, Cordeiro, O, Matos, E, Wulff, T, Jessen, F, Rodrigues, PM. 2010. Post-mortem sarcoplasmic proteomic profile of gilthead seabream is affected by pre slaughter stress levels. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Invited oral communication)

424.Silva, TS, Dias, J, Cordeiro, O, Matos, E, Wulff, T, Jessen, F, Rodrigues, PM. 2010. Post Time-dependent effect of pre-slaughter stress levels on the post-mortem sarcoplasmic proteomic profile of Sparus aurata muscle. 6th Symposium of the Danish Proteomics Society, 7 December, Odense, Denmark.

425.Silveira, CM, Cordeiro, O, Dall'Agnol, L, Moura, I, Moura, JJG, Rodrigues, PM, Almeida, MG. 2010. Study of Desulfovibrio desulfuricans ATCC 27774 respiratory flexibility using a differential in gel electrophoresis approach. 4th EuPA Congress on Clinical Proteomics, 23-27 October, Estoril, Portugal.

426.Simes, DC, Viegas, CSB, Cavaco, S, Cancela, ML, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA. 2010. Study of Gla Rich Protein (GRP) function in pathological calcification processes and its potential for use as a biomarker. IV SPB Clinical Biochemistry Workshop, 29 January, Faro, Portugal. (Invited oral communication)


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427.Simes, DC, Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML. 2010. Gla Rich Protein (GRP), a new biomarker for ectopic calcifications?. BIT’s 3rd Annual Protein and Peptide Conference, 21-23 March, Beijing, China. (Invited oral communication)

428.Soares, C, García, C, Dias, J, Conceição, LEC. 2010. Noise measurements in an aquaculture facility with recirculation system: experimental results. 39th International Congress and Exposition on Noise Control Engineering - INTER-NOISE 2010, 13-16 June, Lisbon, Portugal. (Oral communication)

429.Sousa, I, Coelho, R, Abecasis, R, Gonçalves, JMS, Erzini, K. 2010. Luíz Saldanha Marine Park: assessing the community structure and reserve effect in soft bottoms. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain. (Oral communication).

430.Sousa, JA, Castro-Cunha, M, Marques, JF, Ramos, MF. 2010. Microbiological conditions of a sole (Solea senegalensis) production facility in Portugal. 6th International Symposium on Aquatic Animal Health, 5-9 September, Tampa, Florida, USA.

431.Sousa, JJ, Ruiz, AM, Hooper, AJ, Hanssen, RF, Bastos, LC, Gil, AJ, Galindo-Zaldívar, J, Sanz de Galdeano, C. 2010. Anthropogenic subsidence revealed by MTI in an area of active tectonics: Granada basin. ESA Living Planet Symposium, 28 June-2 July, Bergen, Norway.

432.Sousa, JJ, Ruiz, AM, Hooper, AJ, Hanssen, RF, Bastos, LC, Gil, AJ, Galindo-Zaldívar, J, Sanz de Galdeano, C. 2010. Evaluation of MTI feasibility for monitoring millimetric deformation in mountainous areas. ESA Living Planet Symposium, 28 June-2 July, Bergen, Norway.

433.Sousa, R, Antunes, C, Guilhermino, L. 2010. Can invasive freshwater bivalves be responsible for important changes in ecosystem processes and functions? XV Congress of the Iberian Association of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. (Oral communication)

434.Sousa, R, Ilarri, M, Antunes, C, Guilhermino, L. 2010. Effects of heat waves, invasive species and pollution on the decline of native molluscs in the estuary of Minho River (NW of the Iberian Peninsula). SETAC North America 31st Annual Meeting, 7-11 November, Portland, USA.

435.Sousa, R, Illari, M, Freitas, F, Dias, S, Antunes, C, Guilhermino, L. 2010. Engineering by the invasive Asian clam Corbicula fluminea: impacts on macrozoobenthic assemblages. 6th NEOBITA Conference, 14-17 September, Copenhagen, Denmark. (Oral communication)

436.Sousa, R, Morais, P, Ilarri, M, Sousa, AT, Antunes, C, Guilhermino, L. 2010. Pisidium amnicum in the River Minho: the beginning of the end?. V Iberian Symposium of the River Minho Hydrological Basin, 19-20 November, Vila Nova de Cerveira, Portugal. (Oral communication)

437.Sousa-Pinto, I, Abreu, MH, Pereira, R. 2010. Development of integrated multi-trophic aquaculture in Portugal: the last ten years and perspectives for the future. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Oral communication)

438.Sousa-Pinto, I, Abreu, MH, Pereira, R. 2010. Molti trophic aquaculture in Portugal: 10 years history and perspectives for the future. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Oral communication)

439.Souza-Bastos, LR, Freire, CA, Wilson, JM. 2010. Physiological responses of Lipophrys pholis to exposure to air. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain.


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440.Stegner, A, Caldeira, R, Dong, C. 2010. Island wake asymmetries: from laboratory to numerical modelisation. ECCOMAS CFD 2010 – 5th European Conference on Computational Fluid Dynamics, 14-17 June, Lisbon, Portugal. (Oral communication)

441.Stoichev, T, Baptista, MS, Basto, MCP, Vasconcelos, MTSD. 2010. Use of solid phase microextraction in aquatic toxicology study of alkylphenols. 12th International Symposium on Advances in Extraction Technologies, ExTech 2010, 20-22 September, Poznan, Poland. Book of abstracts, p.60. (Oral communication)

442.Sykes, AV, Almansa, E, Martin, R, Rodriguez, C, Lorenzo, A, Andrade, JP. 2010. Lipid characterization of starved and grass shrimp-fed cuttlefish (Sepia officinalis) during the first 7 days after hatching. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. (Oral communication)

443.Sykes, AV, Domingues, P, Lorenzo, M, Andrade, JP. 2010. The effects of tank colours on growth and survival of cuttlefish (Sepia officinalis) hatchlings and juveniles. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

444.Sykes, AV, Gonçalves, RA, Andrade, JP. 2010. Early weaning of cuttlefish (Sepia officinalis) with dead grass shrimp (Palaemonetes varians) from the first day after hatching. V Jornadas de Acuicultura en el Litoral SurAtlántico, 15-16 April, Huelva, Spain.

445.Sykes, AV, Gonçalves, RA, Andrade, JP. 2010. Early weaning of cuttlefish (Sepia officinalis) with dead grass shrimp (Palaemonetes varians) from the first day after hatching. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

446.Sykes, AV, Quintana, D, Andrade, JP. The effects of light intensity on growth and survival of cuttlefish (Sepia officinalis) hatchlings and juveniles. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal.

447.Sykes, AV. 2010. Aquaculture in Portugal. EAS Aquaculture Europe 2010 – Student workshop, 5-8 October, Porto, Portugal. (Oral communication)

448.Teixeira, C, Magalhães, C, Joye, SB, Bordalo, AA. 2010. Rates and regulation of denitrification and anaerobic ammonium oxidation in estuarine sediments. The Water Research Conference, 11-14 April, Lisbon, Portugal.

449.Teixeira, S, Serrão, E, Arnaud-Haond, S. 2011. Biogeography of a hydrothermal vent shrimp from the Mid Atlantic Ridge. 5th Biennial Meeting of the International Biogeography Society, 7-11 January, Iraklion, Crete, Greece.

450.Tellier, F, Ayerdi, P, Broitman, B, Contreras-Porcia, L, Correa, JA, Destombe, C, Engelen, A, Gomez, G, Lopez, C, Oppliger, VL, Valero, M, Faugeron, S. 2010. Geographic segregation and ecological response to habitat selection in two sibling species of brown seaweeds: importance of the haploid microscopic and diploid macroscopic phases. Speciation 2010 Meeting, 13-15 December, Vienna, Austria.

451.Thabard, M, Hellio, C, Gros, O, Engelen, AH, Schaefer, M, Marechal, JP. 2010. Sargassum polyceratium chemical and physical impact on major coral reef invertebrate recruitment in Martinique (FWI). Biofouling congress, New Castle, United Kingdom.


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452.Tiago, DM, Roberto, V, Laizé, V, Cancela, ML. 2010. miR-20a regulates BMP-2 gene expression during differentiation of fish bone-derived cells. EMBO/EMBL Symposium - The Non-Coding Genome, 13-16 October, Heidelberg, Germany.

453.Torres, NV, Santos, G, Hormiga, JÁ, Sykes, AV, Almansa, E. 2010. Modelling of the body composition of Octopus vulgaris: Application to diet design for the optimization of paralarvae survival rate. Biotec2010, 29 September-1 October, Pamplona, Spain.

454.Torrontegui, O, Garcia, SA, Prestes, ACL, Patarra, RF, Garcia, A, Rosas-Alquicira, EF, Álvaro, NV, Martins, GM, Neto, AI. 2010. The effect of natural vs. artificial substrata on the development of benthonic assemblages inside marinas. International Symposium FloraMac 2010, Ponta Delgada, Azores, Portugal. Book of abstracts, p.70.

455.Urbatzka, R, Castro, LFC, Rocha, MJ, Lobo-da-Cunha, A, Monteiro, RAF, Rocha, E. 2010. Seasonal mRNA expression of urate oxidase in brown trout liver and analysis of transcriptional regulation by different hormones. 25th Conference of European Comparative Endocrinologists, 31 August-4 September, Pécs, Hungary.

456.Urbatzka, R, Reis, B, Cruzeiro, C, Ferreira, C, Monteiro, RAF, Rocha, E, Rocha, MJ. 2010. Evaluation of steroidogenic gene expression in adult zebrafish (Danio rerio) exposed to environmental levels of xenoestrogens found in Douro River estuary, Portugal. 25th Conference of European Comparative Endocrinologists, 31 August-4 September, Pécs, Hungary. (Oral communication)

457.Valente, LMP, Bacelar, M, Oliveira, B, Dias, J, Castro-Cunha, M, Ozório, ROA. 2010. Practical plant-based diets for Senegalese sole juveniles. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1331-1332.

458.Valente, LMP, Bacelar, M, Oliveira, B, Dias, J, Castro-Cunha, M, Ozório, R. 2010. Practical dietary formulations with low fish-derived protein sources for Senegalese sole juveniles. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal. (Oral communication)

459.Valente, LMP, Bower, N, Johnston, IA. 2010. Post-prandial expression of growth-related genes in Atlantic salmon juveniles fasted for one-week and fed a single meal to satiation. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain.

460.Valente, LMP, Cardinal, M, Escórcio, C, Bacelar, M, Rocha, E, Malhão, F, Cornet, J, Donnay-Moreno, C, GouygouBergé, JP, Hussenot, J. 2010. Best indicators for sea bream (Sparus aurata) quality from extensive and semi-intensive systems. International Workshop on Sustainable Extensive and Semi-intensive Coastal Aquaculture in Southern Europe, 20-21 January, Tavira, Portugal. (Oral communication)

461.Valente, LMP, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Bacelar, M, Escórcio, C, Rocha, E, Malhão, F, Cardinal, M. 2010. Chemical, sensory and histological characterization of gilthead sea bream (Sparus aurata) reared in southern Europe under different conditions: Integrated, semi-intensive and extensive system. 9th International Congress on the Biology of Fish, 5-9 July, Barcelona, Spain. (Oral communication)

462.Valente, LMP, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Bacelar, M, Escórcio, C, Rocha, E, Malhão, F, Cardinal, M. 2010. Flesh quality of gilthead sea bream (Sparus aurata) from distinct production systems and geographical locations in Southern Europe: integrated, semi-intensive and extensive systems. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1330. (Oral communication)


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463.Valente, LMP, Linares, F, Villanueva, JLR, Escórcio, C, Borges, P, Alvárez-Blázquez, B, Silva, JMG, Espe, M, Medale, F, Cal, RM, Peleteiro, JB. Dietary protein source or energy levels have no major impact on growth performance or nutrient utilization of ongrowing Senegalese sole. EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1333-1334. (Oral communication)

464.Valente, LMP, Moutou, KA, Conceição, L, Galloway, T, Fernandes, J, Johnston, IA. 2010. What determines growth potential and juvenile quality of farmed fish species? EAS Aquaculture Europe 2010, 5-8 October, Porto, Portugal. Book of abstracts, pp. 1328-1329. (Oral communication)

465.Varandas, S, Teixeira, A, Lopes-Lima, M, Hinzmann, M, Cortes, RMV, Machado, J. 2010. Estudos preliminares de populações de náiades nos sectores terminais dos Rios Tua e Sabor (Bacia Do Douro): análise do habitat e da qualidade da água e sedimentos. XV Iberian Congress of Limnology, 5-9 July, Ponta Delgada, Azores, Portgal.

466.Varandas, S, Teixeira, AT, Lopes-Lima, M, Sobral, C, Cortes, R, Machado, J. 2010. Spatial distribution of freshwater mussels in the hydrographic basins of Rivers Tâmega, Tua and Sabor (River Douro Basin). XV Iberian Congress of Limnology, 5-9 July, Ponta Delgada, Azores, Portgal.

467.Vasconcelos, V, Martins, A, Vale, M, Antunes, A, Azevedo, J, Welker, M, Lopez, O, Montejano, G.

2010. First report on the occurrence of microcystins in planktonic cyanobacteria from Central Mexico. 8th International Conference on Toxic Cyanobacteria, 29 August-4 September, Istambul, Turkey.

468.Vasconcelos, V, Martins, A, Vale, M, Antunes, A, Azevedo, J, Welker, M, Lopez, O, Montejano, G.

2101. Microcystin diversity in blooms and in strains of Microcystis spp. isolated from lakes of Central Mexico. VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica. (Oral communication)

469.Vasconcelos, V, Morais, J, Vale, M. 2010. Microcystins and cyanobacteria trends in a 14 year

monitoring of a temperate eutrophic reservoir (Aguieira, Portugal). VIII Iberian Congress and V Iberoamerican Congress of Environmental Contamination and Toxicology – CICTA 2010, 29 November-4 December, Heredia, Costa Rica.

470.Vaz-Pires, P, Escórcio, C, Ramalho Ribeiro, A, Matos, E, Dias, J. 2010. Optimizing slaughter to obtain higher welfare on farmed fish. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

471.Vaz-Pires, P, Soares, F, Ribeiro, L, Pousão-Ferreira, P, Cunha, ME, Quental Ferreira, C, Ramalho, A, Jesus, A, Conceição, L, Dinis, MT, Yúfera, M, Marino, G, Boglione, C, Cataudella, S, Hussenot, J, Begout, M-L, Makridis, P, Buard, E, Blachier, P. 2010 Certification of non-intensive aquaculture products: SEACASE project proposal. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

472.Vaz-Pires, P, Soares, S, Escórcio, C, Ramalho, A, Matos, E, Dias, J. 2010 Optimizing slaughter to obtain higher welfare on farmed fish. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

473.Vaz-Pires, P. 2010. Codes of conduct and certification on semi-intensive and extensive systems. International Workshop on Sustainable Extensive and Semi-intensive Coastal Aquaculture in Southern Europe, 20-21 January, Tavira, Portugal. (Oral communication)


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474.Vaz-Pires, P. 2010. Slaugthering procedures: fish from extensive and semi-intensive production. International Workshop on Sustainable Extensive and Semi-intensive Coastal Aquaculture in Southern Europe, 20-21 January, Tavira, Portugal. (Oral communication)

475.Veiga, P, Gonçalves, JMS, Erzini, K. 2010. An integrative study on the recreational shore fishing in the south of Portugal. Transversal Workshop on the Monitoring Recreational Fisheries in the GFCM Area, 20-22 October, Palma de Mallorca, Mallorca, Spain. (Oral communication)

476.Veiga, P, Gonçalves, JMS, Erzini, K. 2010. Short-term hooking mortality of three fish species (Sparidae) caught by recreational angling in the south Portugal. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain. (Oral communication)

477.Veiga, P, Xavier, J, Assis, C, Erzini, K. 2010. Diet of the blue marlin, Makaira nigricans, off the south coast of Portugal. XVI Iberian Symposium of Marine Biology Studies, 6-10 September, Alicante, Spain.

478.Ventura, C, Voelker, AHL, Fatela, F. 2010. Benthic foraminifera assemblages as responses to climate variations during MIS 14 to 9 on the mid-depth Portuguese margin. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany.

479.Vicente, A, Campos, A, Guerreiro, PM, Castro, M. 2010. Keeping Norway lobster alive on board crustacean trawlers: Testing the effectiveness of a simple maintenance system. International Meeting on Marine Resources - IMMR'10, 16-17 November, Peniche, Portugal.

480.Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML, Simes, DC. 2010. GRP is a new vitamin K dependent protein and a possible ectopic calcification biomarker with diagnostic implications. BIT’s 3rd Annual Protein and Peptide Conference, 21-23 March, Beijing, China.

481.Viegas, MN, Dias, J, Cancela, ML, Laizé, V. 2010. Effect of essential fatty acids on the proliferation and mineralization of gilthead seabream bone-derived cell lines. EAS Aquaculture Europe 2010 Conference, 5-8 October, Porto, Portugal.

482.Vieira, LR, Bølling, N, Hylland, K, Guilhermino, L. 2010. Growth inhibition of the marine planktonic algae Tetraselmis chuii by nickel and two polycyclic aromatic hydrocarbons. Poster Communication. ICES Annual Science Conference 2010, 20-24 September, Nantes, France. Book of abstracts, p.181.

483.Vieira, LR, Duarte, D, Guilhermino, L. 2010. Acute effects of nickel on two key species of estuaries from the NW Portuguese coast: The algae Tetraselmis chuii and the fish Pomatoschistus microps. ECSA 47 Symposium - Estuarine Coastal Sciences Association, 14-19 September, Figueira da Foz, Portugal. Book of abstracts, p.139.

484.Vieira, LR, Duarte, D, Guilhermino, L. 2010. Short term effects of nickel on marine organisms (Tetraselmis chuii and Pomatoschistus microps). XV Congress of the Iberian Association of Limnology, 5-9 July, Ponta Delgada, Azores, Portugal. Book of abstracts, p.103.

485.Voelker, A, Cavaleiro, C, Rebotim, A, Ventura, C, Hillaire-Marcel, C, Martin, P. 2010. Surface and deep water variations and their impact on the Meridional Overturning Circulation during the mid-Brunhes as revealed at IODP Sites U1313 and U1305. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-10810.


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486.Voelker, A, Salgueiro, E, de Abreu, L. 2010. Hydrographic changes along the western Iberian margin: The need to reconstruct and understand regional climate responses for glacial climate record integration. EGU General Assembly, 3-7 May, Viena, Austria. Geophysical Research Abstracts, Vol. 12, EGU2010-12675.

487.Voelker, A. 2010. MIS 11: The Interglacial and Glacial Inception. 3rd PIGS - Past Interglacials Workshop, 20-22 October, Palisades, USA. (Oral communication)

488.Voelker, AH, de Abreu, L, Hillaire-Marcel, C, de Vernal, A, Hodell, DA. 2010. Overturning Circulation in the North Atlantic Ocean During Marine Isotope Stage 11c. American Geophysical Union - AGU Fall Meeting, 5-9 December, San Francisco, California, USA.

489.Voelker, AHL, Caveleiro, C, Rebotim, A, Martin, P. 2010. Mid-Brunhes surface water changes in the mid-latitude North Atlantic revealed by G. inflata trace element records. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany. (Oral communication)

490.Voelker, AHL, Lebreiro, S. 2010. Millennial-scale changes in deep water properties at the middepth western Iberian margin linked to Mediterranean Outflow Water activity. Deep Water Processes and Products Congress, 16-18 June, Baiona, Pontevedra, Spain. (Invited oral communication)

491.Voelker, AHL, Martin, P, Lebreiro, SM. 2010. Mediterranean outflow water activity on the Western Iberian Margin: evidence from a benthic foraminifer trace element study. FORAMS 2010 – International Symposium on Foraminifera, 5-10 September, Bonn, Germany.

492.Wallenstein, FM, Neto, AI, Rodrigues, AS, Wilkinson, M. 2010. Intertidal Seaweed Communities Compared between Azores and Britain – Lessons for Water Framework Directive Monitoring. 45rd European Marine Biology Symposium, 23-27 August, Edinburgh, Scotland. Book of abstracts, p.31.

493.Wilson, JM, Gonçalves, OM, Castro, LFC. 2010. Revisiting the story of stomach loss in fishes. A case of déjà vu. Annual Main Meeting of the Society for Experimental Biology 2010, 30 June-3 July, Prague, Czech Republic. (Oral communication)

494.Wilson, JM, Gonçalves, OM, Ip, YK, Smolka, AJ, Castro, LFC. 2010. The non-gastric H+/K

+-ATPase:

from lamprey to lungfish. 9th International Congress of Fish Biology, 5-9 July, Barcelona, Spain. (Oral communication)

495.Wilson, JM, Gonçalves, OM, Smolka, A, Castro, LFC. 2010. Loss of the gastric proton pump in birds? Annual Main Meeting of the Society for Experimental Biology 2010, 30 June- 3 July, Prague, Czech Republic.

496.Xavier, ED, Furnas, J, Azevedo, JMN, Mota, G, Teves, L, Neto, AI. 2010. Outdoor growth of Haematococcus pluvialis for astaxanthin production in the Azores. International Symposium FloraMac 2010, 23-25 September, Ponta Delgada, Azores, Portugal. Book of abstracts, p.78. (Oral communication)

497.Xavier, ED; Furnas, J, Teves, L, Mota, G, Neto, AI. 2010. Astaxanthin production from Haematococcus pluvialis using photobioreactors. 8th European Workshop Biotechnology of Microalgae, 7-10 June, Nuthetal, Germany. Book of abstracts, p.32. (Oral communication)


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498.Yun, HY, Molis, M, Engelen, AH, Santos, RO. 2010. The invasive Sargassum muticum might talk native brown seaweeds into chemical defense. 20th International Seaweed Symposium, Ensenada, Mexico. (Oral communication)

499.Yun, HY, Molis, M, Engelen, AH, Santos, RO. 2010. The invasive Sargassum muticum might talk native brown seaweeds into chemical defense. XX International Seaweed Symposium, 22-26 February, Ensenada, Mexico.

COMMUNICATIONS IN NATIONAL SCIENTIFIC MEETINGS

1. Almeida, JR, Gravato, C, Guilhermino, L. 2010. Efeitos da variação de temperatura em juvenis de robalo (Dicentrarchus labrax) avaliados a diferentes níveis de organização biológica. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

2. Almeida, R, Guedes, P, Couto, N, Mucha, AP, Almeida, CMR. 2010. Characterization of the actual state of oil contamination in a beach affected by the Prestige oil spill – the O Rostro beach. IJUP – 3º Encontro de Jovens Investigadores da Universidade do Porto, 17-19 Fevereiro, Porto, Portugal.

3. Antunes, A. 2010. Avanços na genómica evolutiva de espécies ameaçadas de mamíferos. XI Jornadas de Biologia Aplicada, 28-30 Abril, Braga, Portugal. (Oral communication)

4. Aragão, C. 2010. Advances in fish larvae nutrition. CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication).

5. Araújo, NCP, Bringela, A, Veríssimo, E, O’Neill, PM, Cristiano, MLS. 2010. New peptidic cysteine protease inhibitors, 2º Encontro de Química Terapêutica, 28-30 Novembro, Coimbra, Portugal. (Oral communication)

6. Arenas, F, Olabarria, C, Viejo, R, Rubal, M, Veiga, P, Incera, M, Vaz-Pinto, F, Cacavelos, E, Sobrino, C, Sousa Pinto, I. 2010. Climate change and marine Invasions: Experimental insights from macro-algal assemblages. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

7. Arenas, F. 2010. Efeitos da diversidade biológica para além da riqueza de espécies. CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication).

8. Aureliano, M, Ramos, S, Moura, JJG. 2010. New insights into decavanadate protein targets. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

9. Azevedo, C, Samuel, N, Saveia, AP, Delgado, F, Casal, G 2010. Thelohanellus sp. (Myxozoa: Myxosporea) parasita de Rhabdalestes maunensis (Teleostei) do rio Kwanza, Angola. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal.

10. Barreira, L, Silvestre, L, Pereira, H, Vizetto-Duarte, C, Rauter, AP, Alberício, F, Varela, J. 2010. Neuroprotective activity in macroalgal extracts. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

11. Bensimon-Brito, A, Huysseune, A, Witten, PE, Cancela, ML. 2010. Vertebral fusion in zebrafish (Danio rerio) vertebral column the caudal skeleton as a model. Seminários Mar e Ambiente, 17 Dezembro, CCMAR, Faro, Portugal. (Oral communication)


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12. Bentes, L. 2010. Rensub: Biodiversidade marinha no Algarve. Bioblitz Alvor 2010, 4-5 Setembro, Alvor, Portugal. (Oral communication).

13. Bertocci, I. 2010. Forecasting the consequences of climate change on intertidal organisms: experimental manipulations in the field of the intensity and temporal variance of disturbance. Seminários Oceanus, 15 Março, CIIMAR, Porto, Portugal. (Oral communication)

14. Bio, A. 2010. Fish removal in the Furnas Lake, Azores – a failed bio-manipulation attempt. Seminários Oceanus, 24 Maio, CIIMAR, Porto, Portugal. (Oral communication)

15. Borges, MJRC, Power, DM, Cardoso, JCR. 2010. Putative receptors for the parathyroid hormone family in non-vertebrates. CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal.

16. Carvalho, A, Santos, P. 2010. Biodiversidade macrobentónica no estuário do Rio Cávado. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

17. Carvalho, PN, Almeida, CMR, Basto, MCP, Vasconcelos, MTSD. 2010. Can the veterinary drugs ivermectin, enrofloxacin, penicillin and ceftiofur be analyzed by gas chromatography?. 2º Encontro de Químicos Jovens Portugueses - 2PYCheM, 21-23 Abril, Aveiro, Portugal.

18. Casal, G, Abdel-Baki, AS, Dkhil,

M, Al-Qahtani,

H, Al-Quraishy,

S, Azevedo, C. 2010. Ultrastrutura e

filogenia de um microsporídio encontrado no tecido muscular do peixe teleósteo Saurida undosquamis (Synodontidae) proveniente do Golfo Arábico. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal.

19. Casal, G, Azevedo, C. 2010. Caracterização ultrastrutural e molecular de dois microsporídios diagnosticados na ictiofauna portuguesa. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal.

20. Castro, R, Silva, A, Casal, G, Cavaleiro, FI, Santos, MJ. 2010. Estudo comparativo dos ectoparasitas da cavala, Scomber japonicus Houttuyn, 1782 e da sarda, Scomber scombrus Linnaeus, 1758 na costa norte de Portugal Continental. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 85.

21. Cavaleiro, FI, Santos, MJ. 2010. Análise microambiental da infecção de Octopus vulgaris Cuvier, 1797 (Cephalopoda: Octopodidae) por Octopicola superba superba Humes, 1957 (Copepoda: Octopicolidae) na costa Portuguesa. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 92.

22. Cavaleiro, FI, Santos, MJ. 2010. Padrão de ocorrência espacial de Octopicola superba superba Humes, 1957 (Copepoda: Octopicolidae) em polvo comum, Octopus vulgaris Cuvier, 1797 (Cephalopoda: Octopodidae), da costa Portuguesa. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 91.

23. Conceição, L. 2010. Policultivo semi-intensivo: caso de estudo. Workshop Nacional sobre Aquacultura Extensiva e Semi-intensiva: Policultivo, o Caso de Estudo Português, 19 Janeiro, Tavira, Portugal. (Oral communication).

24. Conceição, N, Fazenda, C, Viegas, CSB, Leite, RB, Cancela, ML. 2010. Promoter analysis of cartilage-expressed genes. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal.


332

25. Conceição, N, Lelo, L, Pinto, JP, Cancela, ML. 2010. Regulation of hepcidin expression: in silico promoter analysis. 4th National Meeting on Signal Transduction, 21-22 Maio, Faro, Portugal.

26. Conceição, N, Simão, M, Cancela, ML. 2010. Lab-it, a hands-on molecular biology pilot program implemented in high schools of the Algarve region and aiming to promote training and interest for science among students. XXXV Jornadas Portuguesas de Genética, 31 Maio-2 Junho, Braga, Portugal. (Oral communication)

27. Correia, M, Cavaleiro, FI, Formigo, NE, Santos, MJ. 2010. Restrição de nichos ecológicos no tracto intestinal da solha Europeia Platichthys flesus (Linnaeus, 1758) (Teleostei: Pleuronectidae) por helmintas parasitas. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 62. (Oral communication)

28. Dinis, MT. 2010. Aquacultura extensive e semi-intensiva em Portugal. Workshop Nacional sobre Aquacultura Extensiva e Semi-intensiva: Policultivo, o Caso de Estudo Português, 19 Janeiro, Tavira, Portugal. (Oral communication)

29. Eiras, JC, Takemoto, RM, Pavanelli, GC. 2010. Henneguya sp. (Myxozoa, Myxosporea, Myxobolidae) parasita de Astyanax altiparanae (Osteichthyes, Characidae) no Alto Rio Paraná, Brasil. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal.

30. Eiras, JC. 2010. Biodiversidade dos parasitas de peixes de água doce do Brasil. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. (Oral communication)

31. Erzini, K. 2010. Os benefícios de protecção numa área marinha protegida: o exemplo do parque marinho marinho Professor Luíz Saldanha. " CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication)

32. Fazenda, C, Conceição, N, Cancela, ML. 2010. Identification of two isoforms of gla rich protein (grp) in zebrafish and its molecular characterization. XVII Congresso Nacional de Bioquímica, 15-17 Dezembro, Porto, Portugal.

33. Ferreira, M. 2010. Oxidative stress biomarkers in aquatic species, and applications in environmental monitoring. CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication).

34. Fidalgo, ML, Sousa, L. 2010. Serviços dos ecossistemas: o estuário do rio Minho como caso de

estudo. Jornadas de Ambiente, 8-9 Maio, Caminha, Portugal.

35. Francisco, CJ, Almeida, A, Castro, AM, Pina, S, Russel-Pinto, F, Rodrigues, P, Santos, MJ. 2010. Variabilidade da região ITS1 de Diphtherostomum brusinae (Trematoda: Zoogonidae). XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 129.

36. Francisco, CJ, Bray, RA, Lanfranchi, AL, Almeida, A, Timi, JT, Castro, AM, Santos, MJ. 2010. Caracterização molecular de Prosorhynchus spp. (Trematoda: Bucephalidae). XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 45. (Oral communication)

37. Freedman, H. 2010. Semi-classical molecular dynamics simulations of vibrational energy transfer. Mini-workshop on Computer Simulations in Nanobiological Systems, 27 Maio, Faro, Portugal. (Oral communication)


333

38. Freitas, V. 2010. Temperature dependence of epibenthic predation as regulating mechanism on estuarine bivalves and its effect of the fuelling function of estuaries for migratory birds. Seminários Oceanus, 9 Abril, CIIMAR, Porto, Portugal. (Oral communication)

39. Froufe, E. 2010. Using a molecular tool box to answer several questions related with introductions, genetic diversity, hybridization, phylogenies and comparing phylogeograpic patterns. Seminários Oceanus, 29 Março, CIIMAR, Porto, Portugal. (Oral communication)

40. Gomes, SI, Novais, SC, Gravato, C, Guilhermino, L, Scott-Fordsmand, JJ, Soares, AMVM, Amorim, MJB. 2010. Effects of Cu-nanoparticles versus Cu2+ in Enchytraeus albidus (Oligochaeta): stress biomarker response. Seminários Amostras à 4ª, 17 Fevereiro, Aveiro, Portugal. (Oral communication)

41. Gonçalves, F. 2010. Effects of ammonia on bacterial LPS induction of the acute phase response in Danio rerio. Seminários Oceanus, 18 Outubro, CIIMAR, Porto, Portugal. (Oral communication)

42. Gonçalves, JA, Bastos, L, Granja, H, Pinho, JL, Henriques, R, Magalhães, A. 2010. Monitorização de praias e dunas usando fotografia aérea digital com georreferenciação directa. ESIG-2010 Encontro Nacional de Utilizadores de Informação Geográfica, 10-12 Fevereiro, Oeiras, Portugal. (Oral communication)

43. Gonçalves, JA. 2010. Transformação de coordenadas com grelhas - Geração e implementação no ArcGIS. EUE2010 – Encontro de utilizadores ESRI, 3 Março, Lisboa, Portugal. (Oral communication)

44. Gonçalves, JMS, Ribeiro, J, Monteiro, P, Oliveira, F, Veiga, P, Bentes, L, Erzini, K. 2010. Monitorização da ictiofauna da Ria Formosa. Conferência sobre Biodiversidade Costeira Conservar, Gerindo a Compatibilização de Usos, 28 Julho, Olhão, Portugal. (Oral communication).

45. Gonçalves, JMS. 2010. Biodiversidade Marinha. XVI Jornadas Sobre Ambiente e Desenvolvimento, 21-22 Outubro, Leiria, Portugal. (Oral communication)

46. Granja, H, Bastos, L, Pinho, J, Gonçalves, J, Henriques, R, Bio, A, Mendes, J, Magalhães, A. 2010. Risk assessment in coastal zones based on a comprehensive short term monitoring programme. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal. (Oral communication)

47. Guedes, P, Almeida, R, Couto, N, Mucha, AP, Almeida, CMR. 2010. Characterization of the actual state of oil contamination in a beach affected by the Prestige oil spill – Nemiña beach. IJUP – 3º Encontro de Jovens Investigadores da Universidade do Porto, 17-19 Fevereiro, Porto, Portugal.

48. Guilhermino, L. 2010. Biomarcadores ambientais em Ecologia: avaliação de efeitos combinados de múltiplos stressores e implicações para o funcionamento dos ecossistemas. 12º Encontro Nacional de Ecologia, 18- 20 Outubro, Porto, Portugal. (Oral invited communication)

49. Hermida, M, Saraiva, A, Cruz, C. 2010. Dados preliminares sobre a comunidade de parasitas do Goraz, Pagellus bogaraveo (Teleostei: Sparidae), em águas portuguesas. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica 17(2): 64. (Oral communication)

50. Hermida, M, Saraiva, A, Cruz, C. 2010. Ocorrência de Rhadinorhynchus pristis(Acanthocephala, Rhadinorhynchidae) em goraz, Pagellus bogaraveo, no Arquipélago dos Açores, Atlântico Norte. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica 17(2): 81.


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51. Hinzmann, MF, Antunes, F, Lopes-Lima, M, Machado, J, Costa, P. 2010. Association between environmental microbiota and indigenous bacteria found in hemolymph, extrapallial fluid and mucus of Anodonta cygnea (Linnaeus, 1758). Congresso Português de Malacologia, 13-14 Março, Faro, Portugal.

52. Hinzmann, MF, Lopes-Lima, M, Lima, AP, Machado, J. 2010. Food selectivity versus availability by the freshwater bivalve Anodonta cygnea (1758 Linnaeus) from Mira Lagoon (Portugal). Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

53. Ilarri, M, Freitas, F, Dias, S, Antunes, C, Guilhermino, L, Sousa, R. 2010. The Asian clam Corbicula fluminea as ecosystem engineer: effects in the diversity of benthic macroinvertebrates. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

54. Jerónimo, D, Cruz, C, Moreira, MH, Saraiva, A. 2010. Ocorrência de Dinoflagellata em pregado Psetta maxima (Linnaeus, 1758) de cultura. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica 17(2): 88.

55. Larsen, K. 2010. Tanaidacean crustaceans: Taxonomy, Ecology, Biodiversity and potential as bioindicator organisms. Seminários Oceanus, 19 Fevereiro, CIIMAR, Porto, Portugal. (Oral communication)

56. Leão, P. 2010. Portoamides: new cyanobacterial allelochemicals that influence planktonic community structuring. Seminários Neptuno, 17 Maio, CIIMAR, Porto, Portugal. (Oral communication)

57. Leite, RB, Cancela, ML. 2010. Uncovering an uncommon host-parasite interaction: a transcriptomic approach. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal. (Oral communication)

58. Lima, D. 2010. Molecular Characterization of 17-β Hydroxysteroid Dehydrogenase type 12 (17βHSD12) in the Neogastropod Nucella lapillus. Seminários Neptuno, 22 Março, CIIMAR, Porto, Portugal. (Oral communication)

59. Lima, ML, Machado, JP. 2010. Effects of Cd2+

on the calcium metabolism and shell mineralization of bivalve Anodonta cygnea. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

60. Lobo-da-Cunha, A, Oliveira, E, Alves, A, Santos, T, Sousa, J, Coelho, R, Calado, C. 2010. Estudo comparativo do papo nos opistobrânquios carnívoros Philinopsis depicta e Aglaja tricolorata (Cephalaspidea, Aglajidae). Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

61. Lopes-Lima, M, Lopes, A, Hinzmann, M, Rocha, A, Andrade. J, Machado, J. 2010. The influence of hydrostatic pressure on the shell mineralization of Anodonta cygnea: a comparative study with a hydrothermal vent bivalve Bathymodiolus azoricus. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal.

62. Luis, L, Gravato, C, Guilhermino, L. 2010. Efeitos agudos dos hidrocarbonetos aromáticos policíclicos (naftaleno e pireno) no camarão Palaemon serratus. XII Encontro Nacional de Ecologia, 20-22 Outubro, Porto, Portugal.


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63. Machado, JP, Lopes-Lima, M. 2010. A new model for calcium transport and calcification in freshwater mussels. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

64. Marques, CL, Cancela, ML, Laizé, V. 2010. Implications of BMP2 in skeletal development and bone metabolism using the gilthead seabream as a model organism. Seminários Mar e Ambiente, 17 Dezembro, Faro, Portugal. (Oral communication)

65. Marreiros, P, Bastos, L, Fernandes, MJ. 2010. GNSS Precise Positioning without reference stations. 1as Jornadas de Engenharia Hidrográfica, 21-22 Julho, Lisboa, Portugal. (Oral communication)

66. Melão, A, Reis-Pedro, J, Pereira, MJR, Nunes, P, Aureliano, M, Eriksson, J, Carvalho, E. 2010. Effects of immunossupressive agents on insulin resistance. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal. (Oral communication)

67. Mesquita, SR, Guilhermino, L, Guimarães, L. 2010. Alterações comportamentais e bioquímicas no caranguejo verde, Carcinus maenas após exposição ao fármaco antidepressivo fluoxetine. 12º Encontro Nacional de Ecologia, 20-22 Outubro, Porto, Portugal.

68. Moreira, C, Vasconcelos, V, Antunes, A. 2010. Diversidade genética e estrutura populacional da cianobactéria invasora Cylindrospermopsis raciborskii. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto. (Oral communication)

69. Moreira, C. 2010. Phylogeny and biogeography of cyanobacteria. Seminários Neptuno, 8 Março, CIIMAR, Porto, Portugal. (Oral communication)

70. Nave, S, Lebreiro, S, Kissel, C, Guihou, A, Figueiredo, MO, Silva, TP, Michel, E, Cortijo, E, Labeyrie, L, Voelker, A. 2010. Variações da produtividade oceânica durante o último Interglaciar e a sua relação com a Circulação Thermohalina (Open ocean productivity changes during the Last Interglacial and its relation to the Atlantic Meridional Overturning Circulation). VIII Congresso Nacional de Geologia, 9-16 Julho, Braga, Portugal.

71. Neves, I, Baptista, MS, Vasconcelos,

V, Vasconcelos,

MTSD. 2010. Toxicity of the pharmaceutical

Simvastatin to the cyanobacterium Microcystis aeruginosa: does good manufacturing practice influence the biological response? IJUP – 3º Encontro de Jovens Investigadores da Universidade do Porto, 17-19 Fevereiro, Porto, Portugal.

72. Oliveira, M, Gravato, C, Guilhermino, L. 2010. Efeitos do hidrocarboneto policíclico aromático pireno no góbio comum (Pomatoschistus microps) proveniente da população do estuário do rio Minho. XII Encontro Nacional de Ecologia, 20-22 Outubro, Porto, Portugal.

73. Osswald, J, Carvalho, AP, Guimarães, L, Guilhermino, L. 2010. Biomarkers in rainbow trout (Oncorhyncus mykiss) after acute challenge of pure anatoxin-a. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

74. Pearson, GA, Ramos, AA, Lago-Leston, A, Cánovas-Garcia, F, Cox, C, Augustini, S, Duarte C, Serrão, E. 2010. Genómica ambiental de comunidades polares de fitplâncton. 2ª Reunião Portuguesa de Ciências Polares: O Estado da Investigação Polar em Portugal no Final do IV Ano Polar Internacional, 26 Abril, Lisboa, Portugal. (Oral communication)


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75. Pereira, H, Ugalde, P, Barreira, L, Custódio, L, Vizetto-Duarte, C, Varela, J. 2010. Biochemical engineering approaches for inducing lipid production in marine microalgae. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

76. Pereira, MJR, Palming, J, Svensson, MK, Aureliano, M, Carvalho, E, Eriksson, J. 2010. Effects of immunossupressive agents on glucose and lipid metabolism in human subcutaneous adipocytes. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal.

77. Pina, S, Russell-Pinto, F, Rodrigues, P. 2010. Caracterização morfológica e molecular da metacercária Microphallus primas (Digenea: Microphallidae). XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 44. (Oral communication)

78. Pinheiro, C, Azevedo, J, Campos, A, Vasconcelos, V. 2010. Efeitos de extractos brutos de cianobactérias e de cianotoxinas no crescimento de microalgas. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal. (Oral communication)

79. Pinto da Silva, L, Baptista, MS, Cianca, RCC, Almeida, CMR, Vasconcelos, VM. 2010. Optimization of a capillary electrophoresis method for the determination of the amino acid β-N-methylamino-L-alanine. IJUP – 3º Encontro de Jovens Investigadores da Universidade do Porto, 17-19 Fevereiro, Porto, Portugal.

80. Rafael, MS, Laizé, V, Schüle, R, Cancela, ML. 2010. New insights into the role of FHL2 in cell differentiation and phenotype transition. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

81. Ramos, S, Moura, JJG, Aureliano, M. 2010. Decavanadate mediates actin inactivation, Protein-Protein interactions and Protein Folding Meeting, 20-31 Março, Lisboa, Portugal.

82. Rangel, LF, Santos, MJ. 2010. Um novo método para a amostragem de parasitas Myxozoa no celoma de poliquetas marinhos. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 83.

83. Raux, P, Ramalho, A. 2010. Avaliação sócio-económica da aquacultura não intensiva. Workshop Nacional sobre Aquacultura Extensiva e Semi-intensiva: Policultivo, o Caso de Estudo Português, 19 Janeiro, Tavira, Portugal. (Oral communication)

84. Reis, I, Mucha, AP, Almeida, CMR, Bordalo, A. 2010. Contribution of the microbial community for the degradation of the prestige buried fuel in intertidal beach zones. IJUP – 3º Encontro de Jovens Investigadores da Universidade do Porto, 17-19 Fevereiro, Porto, Portugal.

85. Reis, P, Salgado, MA, Vasconcelos, V. 2010. Chthamalus montagui como biomonitor de contaminação por metais ao longo da costa noroeste de Portugal- resultados preliminares. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

86. Relvas, P. 2010. Physical oceanography of the Atlantic Iberian ecosystem: latest ideas and challenges. Seminários Oceanus, 3 Maio, CIIMAR, Porto, Portugal. (Oral communication)

87. Roberto, V, Tiago, D, Cancela, ML. 2010. Zebrafish as a transgenic in vivo model to analyse bone-related gene function and regulation of expression: Identification and characterization of microRNAs involved in bone metabolism, Seminários Mar e Ambiente, June 18, Faro, Portugal. (Oral communication)


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88. Rosa, JT, Cancela, ML, Laizé, V. 2010. Identification of a short-chain dehydrogenase/reductase in fish associated with tissue mineralization. Seminários Mar e Ambiente, 18 Junho, Faro, Portugal. (Oral communication)

89. Rosa, M, Cravo, A, Relvas, P. 2010. A coastal countercurrent in the south cost of Portugal – Summer 2006. 1as Jornadas de Engenharia Hidrográfica, 21-22 Julho, Lisboa, Portugal. (Oral communication)

90. Salgueiro, E, Voelker, A, Martin, PA, Rodrigues, T, Prabhu, CN, Abrantes, F, Grimalt, JO. 2010. Temperature and nutrients changes during MIS 11c and the Holocene on the Portuguese margin. Ciência 2010 – Encontro com a Ciência e Tecnologia em Portugal, 4-7 Julho. Lisboa, Portugal.

91. Santos, C, Abrantes, F, Rodrigues, T, Voelker, A. 2010. Paleoenvironmental and paleoclimatic conditions in the Tagus River Estuary during the last ~14kyr. Ciência 2010 – Encontro com a Ciência e Tecnologia em Portugal, 4-7 Julho. Lisboa, Portugal.

92. Santos, MJ, Severino, R. 2010. Métodos de controlo de Amyloodinium ocellatum em robalo (Dicentrarchus labrax L.) “amigos” do ambiente. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 60. (Oral communication)

93. Severino, R, Santos, MJ. 2010. Controlo químico do ectoparasita Amyloodinium ocellatum no Robalo (Dicentrarchus labrax). XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 87.

94. Silva, A, Castro, R, Cavaleiro, FI, Santos, MJ. 2010. Estudo comparativo dos endoparasitas da cavala, Scomber japonicus Houttuyn 1782, e da sarda, Scomber scombrus, Linnaeus 1758, da costa Noroeste de Portugal. XIV Congresso Português de Parasitologia, 8-10 Setembro, Porto, Portugal. Acta Parasitológica Portuguesa 17(2): 84.

95. Silva, C. 2010. Nutritional regulation of lipid deposition in blackspot seabream (Pagellus bogaraveo). Seminários Oceanus, 22 Janeiro, CIIMAR, Porto, Portugal. (Oral communication)

96. Silva, DM, Santos, P, Correia, AT. 2010. Discrimination of Trisopterus luscus (Linnaeus 1758) stocks in the northern of Portugal using otolith elemental fingerprints. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal. (Oral Communication)

97. Silva, I, Fazenda, C, Conceição, N, Cancela, ML. 2010. Study of the Xeroderma pigmentosum D (XPD) expression in zebrafish. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

98. Silva, J. 2010. Use of alternative protein sources in diets for Senegalese sole (Solea senegalensis Kaup, 1858) juveniles. Seminários Oceanus, 10 Maio, CIIMAR, Porto, Portugal. (Oral communication)

99. Simão, MF, Leite, RB, Cancela, ML. 2010. Identification and characterization of two Ferritin isoforms in clam R. decussatus and evaluation of their Fe2+ oxidation potential. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

100.Simes, DC, Viegas, CSB, Cavaco S, Cancela, ML, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA. 2010. Study of Gla Rich Protein (GRP) function in pathological calcification processes and its potential for use as a biomarker. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal. (Oral communication)


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101. Simes, DC, Viegas, CSB, Cavaco, S, Neves, PL, Ferreira, A, João, A, Williamson, MK, Price, PA, Cancela, ML. 2010. Study of Gla Rich Protein (GRP) and its potential for use as a biomarker of pathological calcification related diseases. Conferências da Sociedade Portuguesa de Biofisica, 20 Outubro, Lisboa, Portugal. (Invited oral communication)

102. Simões, B, Conceição, N, Kelsh, R, Cancela, ML. 2010. Runx3 promoter analysis and expression

patterns in tissues and during development of zebrafish. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

103. Sousa, M, Fazenda, C, Conceição, N, Cancela, ML. 2010. Matrix Gla Protein is a target gene for transcription factor MEF2C. Ciência 2010 – Encontro com a Ciência e Tecnologia em Portugal, 4-7 Julho. Lisboa, Portugal.

104. Sousa, R, Antunes, C, Byers, JE, Guilhermino, L. 2010. Combined effects of extreme events and non-native invasive species on estuarine biodiversity and ecosystem functioning. Ciência 2010 – Encontro com a Ciência e Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication)

105. Sousa, R. 2010. Invasive freshwater bivalves: ecological lessons for European ecosystems. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal. (Oral communication)

106. Sousa, R. 2010. Invasive species and their impacts on ecosystem functioning. Conferências do CMIA Matosinhos, Matosinhos, Portugal. (Invited oral communication)

107.Stoichev, T. 2010. Interactions of of emerging organic contaminants with cyanobacterium Microcystis aeruginosa. Seminários Oceanus, 15 Novembro, CIIMAR, Porto, Portugal. (Oral communication)

108.Sykes, AV, Gonçalves, RA, Andrade, JP. 2010. Early weaning of cuttlefish (Sepia officinalis) with dead grass shrimp (Palaemonetes varians) from the first day after hatching. Congresso Português de Malacologia, 13-14 Março, Faro, Portugal.

109.Teixeira, AT, Lopes-Lima, M, Machado, J, Hinzmann, M, Cortes, R, Varandas, S, Antunes, F. 2010. Estudos preliminares de populações de mexilhão-de-rio (Margaritifera margaritifera L.) nos Rios Rabaçal e Tuela (Nordeste de Portugal): análise do habitat e da qualidade da água e sedimentos 10º Congresso da Água, 21-24 Março, Alvor, Portugal.

110. Tiago, DM, Cancela, ML, Laizé, V. 2010. New insights into vanadate action in bone formation: signalling mechanisms and global gene expression analysis. 4th National Meeting on Signal Transduction, 21-22 Maio, Faro, Portugal.

111. Tiago, DM, Laizé, V, Bargelloni, L, Ferraresso, S, Romualdi, C, Cancela, ML. 2010. New insights into anti-mineralogenic effect of vanadate using global gene expression approach. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal.

112. Tiago, DM, Roberto, V, Laizé, V, Cancela, ML. 2010. New insights into regulatory role of microRNAs in bone formation: bone morphogenetic protein 2 transcript is targeted by microRNA-20a. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal. (Oral communication)

113. Tiago, T, Silva, D, Aureliano, M, Guitiérrez-Merino, C. 2010. Cross-talk between actin cytoskeleton dynamics and calcium homeostasis in cerebellar granule neuros esposed to oxidative/nitrosative stress. 4º Workshop de Bioquímica Clínica, 29 Janeiro, Faro, Portugal.


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114. Tiago, T, Silva, D, Samhan-Arias, AK, Aureliano, M, Guitiérrez-Merino, C. 2010. Cross-talk between actin cytoskeleton dynamics and calcium homeostasis in cerebellar granule neurons: sensitivity to oxidative stress. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

115. Valente, LMP, Escórcio, C, Bacelar, M, Rocha, E, Malhão, F, Cornet, J, Donnay-Moreno, C, Gouygou, JP, Bergé, JP, Hussenot, J, Cardinal, M. 2010. Indicadores de qualidade em douradas provenientes de sistemas extensivos e semi-extensivos. Workshop Nacional de Aquacultura Costeira Sustentada Extensiva e Semi-intensiva no Sul da Europa, 19 Janeiro, Tavira, Portugal. (Oral communication)

116. Vasco, EC, Cravo, A, Relvas, P. 2010. Caracterização hidrográfica e química de longo termo na região costeira de Benguela. 1as Jornadas de Engenharia Hidrográfica, 21-22 Julho, Lisboa, Portugal. (Oral communication)

117. Vasconcelos, V. 2010. Impacte ambiental de toxinas de cianobactérias. 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal. (Invited oral communication)

118. Vaz-Pires, P. 2010. Codes of Conduct and Certification in non-intensive aquaculture systems. Workshop Nacional de Aquacultura Costeira Sustentada Extensiva e Semi-intensiva no Sul da Europa, 19 Janeiro, Tavira, Portugal. (Oral communication)

119. Vaz-Pires, P. 2010. Seafood handling, preservation and quality. National course on Seafood Production and Technology, 23 Abril, Bragança, Portugal. (Oral communication)

120. Vaz-Pires, P. 2010. Sensory methods for seafood quality evaluation: the Quality Index Method (QIM). National workshop on New Seafood Products: Innovation and Valorization of Fish and Aquaculture Products + Product Show, 20 Maio, Aveiro, Portugal.

121. Vieira, LR, Morgado, F, Guilhermino, L. 2010. Dados preliminares sobre a estrutura e composição do zooplâncton em dois estuários da costa noroeste de Portugal com diferentes níveis de poluição (estuários dos rios Minho e Lima). 12º Encontro Nacional de Ecologia, 18-20 Outubro, Porto, Portugal.

122. Vizetto-Duarte, C, Custódio, L, Barradas, A, Pereira, H, Barreira, L, Varela, J. 2010. Microalgae as a potential source of new drugs for cancer treatment. XVII Encontro da Sociedade Portuguesa de Bioquímica, 15-17 Dezembro, Porto, Portugal.

123. Voelker, A. 2010. Paleoclimatic changes recorded in deep-sea sediments. CIÊNCIA 2010 – Encontro com a Ciência e a Tecnologia em Portugal, 4-7 Julho, Lisboa, Portugal. (Oral communication)

124. Wilson, J. 2010. Stomach loss in fishes. Insights from the gastric proton pump genes. Seminários Oceanus, 11 Outubro, CIIMAR, Porto, Portugal. (Oral communication)

D. PATENTS

1. Cruzeiro, L. Método para deduzir a estrutura tridimensional de uma proteína a partir da sequência de aminoácidos. Portuguese provisional patent Application nº 105299, 20/09/2010.


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2. Costa, MC, Barros, RJ, Martins, MS, Jesus, C. Uso de lama de mármore para pré-tratamento químico de efluentes ácidos. National patent nr. 103894, 06/08/2010.

3. Costa, MC, Barros, RJ, Martins, MS, Jesus, C. Uso de resíduos vinícolas como fonte de carbono e de energia para bactérias sulfato-redutoras. National patent nr. 104182, 22/11/2010.

4. Brito, AB, Cancela, ML, Gavaia, PJ, Laizé, V. Identification of compound that affects osteogenesis, involves inducing fin regeneration of ray-finned fish by amputating fish fin, contacting fish with test compound, and determining re-mineralization index of fin regeneration. Patent nrs: WO2010044689-A2, WO2010044689-A3, 22/04/2010 and 10/6/2010.

5. Danielsen, S, Skov, LK, Leite, R, Laizé, V, Cancela, ML. New isolated polypeptide having organophosphorous hydrolase activity selected from polypeptide containing amino acid sequence, polypeptide encoded by polynucleotide, variant containing deletion of amino acids, used in nucleic acid construct. Patent nrs. WO2010128115-A1, EP2248893-A1, 11/11/2010.

6. Danielsen, S, Skov, LK, Leite, R, Laizé, V, Cancela, ML. New isolated polypeptide with organophosphorus hydrolase activity selected from e.g. polypeptide with amino acid sequence having specific identity to mature polypeptide and polypeptide encoded by polynucleotide, used in such as composition. Patent nrs. WO2010128116-A1, 11/11/2010.

7. Simes, DC, Viegas, CSB, Cancela, ML. A gamma-carboxyglutamate-rich protein, methods and assays for its detection, purification and quantification and uses thereof. Patent nrs. WO2010024704, 3/03/2010.


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Information compiled by:

Susana Moreira CIIMAR Science Office Rua dos Bragas 289 4050-123 Porto Portugal Phone: +351 22 339 06 07 E-mail: [email protected]

Please use the above contact for data improvement.

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