Why AMAFERM® in the ration?Why AMAFERM® in the ration?Why AMAFERM in the ration?Why AMAFERM in the ration?
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERMAMAFERM®®AMAFERMAMAFERM
Application & Value Application & Value ppin Dairy Cattle
ppin Dairy Cattle
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Har estingHar estingHybrid SelectionHybrid Selection
HarvestingHarvesting
StorageStorage
Producer investments in forageProducer investments in forageProducer investments in forage Producer investments in forage digestibility!digestibility!
Processing / MixingProcessing / MixingBunk ManagementBunk Management
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
$=$= $$$$Har estingHar esting
$=$= $=$=$=$=Hybrid SelectionHybrid Selection
HarvestingHarvesting
StorageStorage
Why Stop Here?Why Stop Here?Why Stop Here?Why Stop Here?
$=$=$=$=Processing / MixingProcessing / MixingBunk ManagementBunk Management
$$$$
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
“The Fiber Digestion Additive”“The Fiber Digestion Additive”“The Fiber Digestion Additive”“The Fiber Digestion Additive”
What will your clients What will your clients choose?choose?
ThisThis ThisThis
OrOr
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERMAMAFERM®® Description:• A natural feed additive obtained from the controlled fermentation
of Aspergillus oryzae utilizing a proprietary, multi-step process to achieve optimized product activity
• Produced in a dedicated fermentation facility. Compliant with ISO 9000, Safe Feed Safe Food certification, and HACCP certification
Involved in a process of continued improvement to further increase• Involved in a process of continued improvement to further increase product potency and efficacy
• Offers the ruminant industry one of the largest independent d t b f bli h d h ltdatabases of published research results
• One of the only feed additives produced specifically to maximize the digestion of ration fiber components
• Available in Granular, Liquid, and Organic Forms
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
TheThe AMAFERMAMAFERM® Advantage• In the rumen:
– Stimulates Rumen Fungi• Impacts rate and extent of fiber digestion• Impacts rate and extent of fiber digestion
– Stimulates fiber digesting bacteria• Impacts rate and extent of fiber digestion
I i bi l t i i ld• Increases microbial protein yield – Stimulates lactate utilizing bacteria
• Minimizes negative impact of rumen pH
• In the cow:– Increases milk per pound of feed consumed– Increases component yield and body condition– Resulting in increased return over feed costs
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERMAMAFERM®®
AdvantageAdvantageForagesForages NonNon--forage Fiberforage Fiber
NDF ComponentsNDF ComponentsAmaferm Amaferm I tI t
Fungal digestionFungal digestionRumenRumen
ImpactsImpacts
Bacterial digestionBacterial digestion
Resulting benefits: Resulting benefits: •• Increased EnergyIncreased Energy•• Higher Quality ProteinHigher Quality Protein
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
WithoutWithout AMAFERMAMAFERMAMAFERMAMAFERM®®
AdvantageAdvantageAdvantageAdvantageForagesForages NonNon--forage Fiberforage Fiber
NDF ComponentsNDF Components
Amaferm Amaferm ImpactsImpacts
With With AMAFERMAMAFERMFungal digestionFungal digestion
Bacterial digestionBacterial digestionRumenRumen
ImpactsImpacts
Resulting benefits:Resulting benefits:Resulting benefits: Resulting benefits: •• Increased EnergyIncreased Energy•• Higher Quality ProteinHigher Quality Protein
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERMAMAFERM®® Delivers More Enzyme Delivers More Enzyme Production Where it is Needed Most!Production Where it is Needed Most!
AMAFERMAMAFERM Control
Production Where it is Needed Most!Production Where it is Needed Most!
12 2500 12 2500
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
12 µm x 2500
Source: Chang et al, 1999 J. of Applied Microbiology 86:389-398
12 µm x 2500
AMAFERMAMAFERM®® Delivers More Enzyme Delivers More Enzyme Production Where it is Needed Most!Production Where it is Needed Most!
AMAFERMAMAFERM Control
Production Where it is Needed Most!Production Where it is Needed Most!EnzymeEnzyme
EE
EnzymeEnzymeEnzymeEnzyme
EnzymeEnzyme
EnzymeEnzyme
EnzymeEnzyme
EnzymeEnzyme
12 2500
EnzymeEnzyme
12 2500
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
12 µm x 2500
Source: Chang et al, 1999 J. of Applied Microbiology 86:389-398
12 µm x 2500
Fungi Attached to Fungi Attached to FiberFiber
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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Fungal Effect on Dry MatterFungal Effect on Dry MatterFungal Effect on Dry MatterFungal Effect on Dry Matter
WithW/oTime ofDry Matter Loss %
12 36 0
With Fungi
25 7
W/o Fungi
Time of Incubation, hr
24
12
46 4
36.0
38 7
25.7
36
24
48.6
46.4
42.4
38.7
48 53.844.6
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Elliot et al., 1987. J. Agric. Sci. Camb. 109:13-17
AMAFERM® – Impact on Milk AMAFERM® – Impact on Milk Production Production
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Number Lacatation
Milk ProductionMilk ProductionMilk ProductionMilk Productionof Cows Stage Control Amaferm % Diff.
Harris 32 Mid 54.4 57.3 5.3Van Horn 32 Early-Mid 53.9 57.6 6.9Huber 12 Mid 40 7 44 4 9 1Huber 12 Mid 40.7 44.4 9.1
12 Mid 42.7 41.1 <3.7>Wallentine 50 Early 66.2 74.1 12.0
100 Overall 65.1 67.6 3.8Marcus 195 Early-Mid 63.8 67.5 5.9
University Published ResultsMarcus 195 Early Mid 63.8 67.5 5.9Kellems 32 Early 78.3 85.6 9.2
32 Early-Mid 79.4 84.0 5.832 Mid 73.3 76.3 4.2
Kellems 96 Early-Mid 60.6 63.4 4.6Si t 12 E l 77 2 78 1 0 1
4.73% average increase
11 00% increase in early lactationSievert 12 Early 77.2 78.1 0.112 Early 75.9 76.6 0.1
Gomez 24 Mid 49.1 51.0 4.046 Early 85.1 88.9 4.4
Dennigan 40 Early 67.9 67.8 <0.1>
11.00% increase in early lactation
6.40% increase during heat stressDennigan 40 Early 67.9 67.8 0.1
24 Mid 57.7 57.1 <0.1>Baumgard 22 Early 89.7 98.4 9.7
Ave. Average/trial 65.6 68.7
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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Average percent increase Average percent increase 4.73%
AMAFERM® – Impact on FiberAMAFERM® – Impact on FiberAMAFERM – Impact on FiberAMAFERM – Impact on Fiber
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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Factors Effecting Fiber DigestionFactors Effecting Fiber Digestion
• NDF level and digestibility* – Plant type and structure yp
• Feed processing • Microbial populations*p p
– Fungi and Bacteria• Rate of passage*p g• Rumen pH*
**
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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Relationship between Relationship between NDFdNDFd & Performance& Performance
• Each 1% change in NDFd can change Dry Matter Intake .37 lbs1
Each 1% change in NDFd can change milk production• Each 1% change in NDFd can change milk production by .55 lbs of 4% FCM1
• Subsequent research has shown that in higherSubsequent research has shown that in higher lactating cows this difference could approach 2.0 lbs of milk per 1% unit of NDFd change2
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA1Source: Oba & Allan, 1999 JDS 82:589-5962Source Mike Allen Ph.D., “Time to Regroup”
Effect of Effect of AMAFERMAMAFERM®® on Rumen on Rumen Fungal Growth & Fungal Growth & CellulaseCellulase ActivityActivity
200
P< 0.05
Control
AMAFERM27% I
87% Increase
100
150
P< 0.05
AMAFERM27% Increase
50
0Fungal Cell Mass Cellulase
C %
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Source: Welch, 1996, Appl. Microbiol Biotechnol 45:811-821
Control set at 100%
AMAFERMAMAFERM®® Effect on Rumen Bacteria
188% Increase
250
300P<0.001 Control
AMAFERM79% Increase
150
200AMAFERM
50
100
0
Total Bacteria Cellulolytic Bacteria
C %
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Frumholtz, 1989, J. Agric. Sci. 113:169-172
Control set at 100%
AMAFERMAMAFERM®® Impact on Impact on Bacterial Growth RateBacterial Growth RateBacterial Growth Rate Bacterial Growth Rate
Control AMAFERM Control AMAFERM
Growth Rate Doubling Time (min.)
Fibrobacter succinogenes S85 0.26b 0.35a 155 125Megasphaera elsdenii B159 0.32b 0.43a 130 99
M elsdenii T81 0 30b 0 40a 150 115M. elsdenii T81 0.30 0.40 150 115
M. elsdenii LC1 0.29d 0.32c 141 129Ruminococcus albus 7 0.58b 0.72a 72 60Selenomonas ruminantium D 0 59b 0 71a 70 61Selenomonas ruminantium D 0.59b 0.71a 70 61Sel. ruminantium HD1 0.65b 0.75a 66 53Sel. ruminantium HD4 0.62b 0.74a 69 54S l i ti GA31 0 50a 0 54a 85 80Sel. ruminantium GA31 0.50a 0.54a 85 80Sel. lactilytica PC18 0.57b 0.72a 73 60
a b ithi ith diff t i t diff (P < 0 01) c d ithi ith diff t i t diff (P < 0 05)
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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a,b means within a row with different superscripts differ (P < 0.01) c,d means within a row with different superscripts differ (P < 0.05)
Source: Beharka and Nagaraja, 1998. J. Dairy Sci. 81:1591-1598
DirectDirect--fed Microbial Productsfed Microbial ProductsProduct Tested Comments or Animal
TrialsTrials
AMAFERM®
(multi-stage ferment)Largest Research
Database, Multi-stage Fermentation Process
ATCC, 458Aspergillus oryzae
Single Stage Ferment
Commercial Aspergillus(Product A)
Inconsistent Quality/Supply
Difco Saccharomyces(B127)
Public Yeast Species
Commercial Saccharomyces(Product D)
Yeast Culture
Commercial Saccharomyces(Product P)
Live Yeast
Commercial Saccharomyces(Product S)
Live Yeast
Commercial Saccharomyces(Product MT)
Live Yeast
Commercial Lactobacillus(Product BM)
Limited Research
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Source: Calza, Washington State Univ.
(Product BM)
DirectDirect--fed Microbial Productsfed Microbial ProductsProduct Tested Comments or Animal
TrialsStimulation of
BacterialTrials BacterialGrowth
AMAFERM®
(multi-stage ferment)Largest Research
Database, Multi-stage Fermentation Process
3.2 timesIncrease
ATCC, 458Aspergillus oryzae
Single Stage Ferment
1.8 - 2.0 timesIncrease
Commercial Aspergillus(Product A)
Inconsistent Quality/Supply
2.0 times increase
Difco Saccharomyces(B127)
Public Yeast Species Set at standard
1X
Commercial Saccharomyces(Product D)
Yeast Culture 3.0 timesIncrease
Commercial Saccharomyces(Product P)
Live Yeast Minimal
Commercial Saccharomyces(Product S)
Live Yeast Variable
Commercial Saccharomyces(Product MT)
Live Yeast Variable, minimal
Commercial Lactobacillus(Product BM)
Limited Research Some, minimal
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Calza, Washington State Univ.
(Product BM)
DirectDirect--fed Microbial Productsfed Microbial ProductsProduct Tested Comments or Animal
TrialsStimulation of
BacterialStimulation of Bacterial FiberTrials Bacterial
GrowthBacterial Fiber
Degrading Enzymes
AMAFERM®
(multi-stage ferment)Largest Research
Database, Multi-stage Fermentation Process
3.2 timesIncrease
13 - 49% increase
ATCC, 458Aspergillus oryzae
Single Stage Ferment
1.8 - 2.0 timesIncrease
Minimal
Commercial Aspergillus(Product A)
Inconsistent Quality/Supply
2.0 times increase
Minimal, none
Difco Saccharomyces(B127)
Public Yeast Species Set at standard
1X
Set at standard1X
Commercial Saccharomyces(Product D)
Yeast Culture 3.0 timesIncrease
Minimal
Commercial Saccharomyces(Product P)
Live Yeast Minimal No effect
Commercial Saccharomyces(Product S)
Live Yeast Variable Not tested
Commercial Saccharomyces(Product MT)
Live Yeast Variable, minimal
Not tested
Commercial Lactobacillus(Product BM)
Limited Research Some, minimal Not tested
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Calza, Washington State Univ.
(Product BM)
DirectDirect--fed Microbial Productsfed Microbial ProductsProduct Tested Comments or Animal
TrialsStimulation of
BacterialStimulation of Bacterial Fiber
Stimulation of Fungal Physiology and FiberTrials Bacterial
GrowthBacterial Fiber
Degrading EnzymesPhysiology and Fiber Degrading Enzymes
AMAFERM®
(multi-stage ferment)Largest Research
Database, Multi-stage Fermentation Process
3.2 timesIncrease
13 - 49% increase 2.5 times increase
ATCC, 458Aspergillus oryzae
Single Stage Ferment
1.8 - 2.0 timesIncrease
Minimal Minimal
Commercial Aspergillus(Product A)
Inconsistent Quality/Supply
2.0 times increase
Minimal, none No effect
Difco Saccharomyces(B127)
Public Yeast Species Set at standard
1X
Set at standard1X
No effect
Commercial Saccharomyces(Product D)
Yeast Culture 3.0 timesIncrease
Minimal No effect, minimal
Commercial Saccharomyces(Product P)
Live Yeast Minimal No effect Minimal
Commercial Saccharomyces(Product S)
Live Yeast Variable Not tested Variable
Commercial Saccharomyces(Product MT)
Live Yeast Variable, minimal
Not tested No effect
Commercial Lactobacillus(Product BM)
Limited Research Some, minimal Not tested Not tested
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Calza, Washington State Univ.
(Product BM)
Lactating Dairy Cows’ Lactating Dairy Cows’ Response to Yeast ProductsResponse to Yeast ProductsResponse to Yeast ProductsResponse to Yeast Products
• Sited results contained within a meta analysis summarizing six diff t t d t (YP) 22 i ddifferent yeasts products (YP) across 22 peer reviewed publications
• All studies were pooled and simple correlations showed that:p p
– Higher diet NDF or ADF levels reduced responses to YP
• Results suggest that % increase in NEL output increased gg L pmodestly in diets with higher starch levels:
– But decreased in diets with higher NDF levels
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Source: Robinson and Erasmus, 2007 JDS Vol. 90 Suppl. 1 No. 362
Impact ofImpact of Ether / Ester Bonds?Item Coastal Tifton 85CP, % of DM 14.5 14.3,ADF, % of DM 40.3 42.3NDF, % of DM 70.9b 75.1a
ADL, % of DM (lignin) 4.3 4.0Ester-linked FA, g/kg CW 12.6b 13.9a
Ether-linked FA, g/kg CW 8.1b 6.9a
OMI, lb/d 9.7 9.9TT OMI digestion, % 54.2b 59.1a
TT ADF digestion, % 51.4b 56.3a
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Source: Mandebvu et al., 1999. JAS 77 (6) 1572-1586
a,b Within a row within cultivar or age, means without a common superscript letter differ (P <0.05)
WithoutWithout AMAFERMAMAFERM With With AMAFERMAMAFERM
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BioZyme Incorporated
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BioZyme Incorporated
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Cell Wall Digestion Cell Wall Digestion Cell Wall Digestion Cell Wall Digestion
C l i tiGlycoclax
Colonization
C llCell
Wall EnzymeEnzyme
EnzymeEnzyme
Dots—represent cell wall degrading enzymes produced by the bacterium
Open blocks—represent structural polysaccharides not associated with lignin
Grey blocks—low Lignin-CHO complex
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source:Chesson (1993)
Black blocks—high Lignin-CHO complex
AMAFERMAMAFERM®® NDF Degradation NDF Degradation AMAFERMAMAFERM®® NDF Degradation NDF Degradation Forage % Improvement ResearcherForage % Improvement Researcher
Alfalfa 12 Berharka and Nagaraja
Alfalfa 18 NocekAlfalfa 18 Nocek
Bromegrass 28 Varel and Kreikemeier
Bromegrass 8 Berharka and Nagaraja
Bromegrass 12 Varel et al.
Corn silage 34 Nocek
F 8 B h k d N jFescue 8 Berharka and Nagaraja
Prairie Hay 30 Westvig et al.
Straw 66 Fondevilla et alStraw 66 Fondevilla et al.
Switchgrass 12 Varel and Kreikemeier
TMR 13 Gomez et al.
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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AMAFERMAMAFERM®® Impact on Impact on Rate of DigestionRate of Digestion
45
50
Rate of DigestionRate of Digestion
Amaferm*Amaferm* ControlControl
30
35
40 Amaferm*Amaferm* ControlControl
Rate of DigestionRate of Digestion
20
25
Extent of DigestionExtent of Digestion
ate o gest oate o gest o
5
10
15
00 24 48 72
P < 0 05 Substrate - Wheat Straw
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
P < 0.05
Source: Fondevila et al., 1990 Animal Prod. 422-425
Substrate - Wheat Straw
AMAFERM®AMAFERM® Impact on Impact on Extent of DigestionExtent of Digestion
20at
12h
Extent of DigestionExtent of Digestion
A fA f k i kl tk i kl t
15
rom
egra
ssa
b
bb
Control
AMAFERM
Amaferm Amaferm works quickly to works quickly to increase digestibilityincrease digestibility
10
adat
ion
of B
r b
aa
AMAFERM
5
In v
itro
Deg
ra a
0(%) I
NDF Hemicellulose Celluloseff f ff ( )
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Varel et al., 1993. Applied and Environmental Microbiology. Pg 3171-3176
Means with different letters are significantly different (P < 0.01)
Relationship between Relationship between NDFdNDFd & Performance& Performance
• Each 1% change in NDFd can change Dry Matter Intake .37 lbs1
Each 1% change in NDFd can change milk production• Each 1% change in NDFd can change milk production by .55 lbs of 4% FCM1
• Subsequent research has shown that in higherSubsequent research has shown that in higher lactating cows this difference could approach 2.0 lbs of milk per 1% unit of NDFd change2
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA1Source: Oba & Allan, 1999 JDS 82:589-5962Source Mike Allen Ph.D., “Time to Regroup”
AMAFERMAMAFERM®® –– Early LactationEarly LactationIt C t l AMAFERMItem Control AMAFERM
DMI, % of BW 3.95 4.2** P < 0.05
**P < 0.10
Digestibility,%
DM 64.0 71.9*
OM 65.3 72.9*
CP 70.5 77.6*
NDF 50.7 57.1* 6.4%
ADF 40.3 48.6*
Milk (FCM) 75 02 78 98** 3 96 lbsBioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Milk (FCM) 75.02 78.98 3.96 lbs
Source: Gomez-Alarcon et al., J. of An. Sci. 69:1733-1740
AMAFERMAMAFERM®® –– Early LactationEarly LactationIt C t l AMAFERMItem Control AMAFERM
DMI, % of BW 3.95 4.2** P < 0.05
**P < 0.10
Digestibility,%
DM 64.0 71.9*1% increase in NDFd is equal to .55 lbs of FCM
57 1% 50 7% = 6 4% X 55 = 3 52 lbs FCMOM 65.3 72.9*
57.1% – 50.7% = 6.4% X .55 = 3.52 lbs FCM
Trial Result 78.98 lbs - 75.02 lbs = 3.96 lbsCP 70.5 77.6*
NDF 50.7 57.1* 6.4%
Oba and Allen, 1999 J. Dairy Sci 82:589-596
ADF 40.3 48.6*
Milk (FCM) 75 02 78 98** 3 96 lbsBioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Milk (FCM) 75.02 78.98 3.96 lbs
Source: Gomez-Alarcon et al., J. of An. Sci. 69:1733-1740
AMAFERMAMAFERM®® –– Early LactationEarly LactationIt C t l AMAFERMItem Control AMAFERM
DMI, % of BW 3.95 4.2** P < 0.05
**P < 0.10
Digestibility,%
DM 64.0 71.9*1% increase in NDFd is equal to .55 lbs of FCM
57 1% 50 7% = 6 4% X 55 = 3 52 lbs FCMOM 65.3 72.9*
57.1% – 50.7% = 6.4% X .55 = 3.52 lbs FCM
Trial Result 78.98 lbs - 75.02 lbs = 3.96 lbsCP 70.5 77.6*
NDF 50.7 57.1* 6.4%
Oba and Allen, 1999 J. Dairy Sci 82:589-596
3.96 lbs is 5.2% increase ADF 40.3 48.6*
Milk (FCM) 75 02 78 98** 3 96 lbs
University milk trial average is 4.73%
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Milk (FCM) 75.02 78.98 3.96 lbs
Source: Gomez-Alarcon et al., J. of An. Sci. 69:1733-1740
Spruce Haven FarmsSpruce Haven FarmsAuburn, NYAuburn, NY,,
• Trial Design– 4 alfalfa samples, 4 corn silage samples
Lo/Hi NDF Lo/Hi NDFd• Lo/Hi NDF, Lo/Hi NDFd– 21 days adaptation period prior to each treatment– Control versus 5 gm and 15 gm Amaferm treatment
Treatment design (Alfalfa hay)NDF Lignin NDFd ADFNDF Lignin NDFd ADF
Lo/Lo 34.4 5.9 44.5 27.0Lo/Hi 34.7 6 50.4 27.9Hi/Lo 43.7 7.6 37.1 35.2Hi/Hi 43.1 7.1 46.0 33.8
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BioZyme Incorporated
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NDF Digestion (Rate)NDF Digestion (Rate)NDF Digestion (Rate)NDF Digestion (Rate)r h
our)
Kd
(% p
erN
DF
K
Lo/Lo Lo/Hi Hi/Lo Hi/Hi
Alfalfa 80 6% Kd Increase
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Alfalfa 80.6% Kd Increase
NDF Digestion (Extent)NDF Digestion (Extent)NDF Digestion (Extent)NDF Digestion (Extent)de
d%
Deg
rad
ND
F %
Lo/Lo Lo/Hi Hi/Lo Hi/Hi
Alfalfa 18.5% Increase 48 hrs
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BioZyme Incorporated
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Alfalfa 18.5% Increase 48 hrs
NocekNocek 2 2 NocekNocek 2 2
• Objective:– Build on the results from the previous in situ trialBuild on the results from the previous in situ trial– Build a large data base to increase confidence
• 75 samples each forage F th i ti t A f Sil d H l– Further investigate Amaferm on Silage and Haylage
• Across a wide range of qualities
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BioZyme Incorporated
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HaylageHaylage ResultsResultsHaylageHaylage ResultsResults
60 P <0 01
Haylage NDF degradation
40
50
60
P <0.01
P <0.01de
d)
Control
AMAFERM
20
30
40P <0.01
F (%
Deg
ra AMAFERM
0
10
20
0 12 24 36
ND
F
0
44.9% Improvement at 12 hours
Overall increased by 11%
0 12 24 36
Hours ControlAmaferm
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BioZyme Incorporated
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Overall increased by 11%
NDF SummaryNDF SummaryNDF SummaryNDF Summary
NDF Degradation NDF Kd
Corn Silage Haylage
Corn silage 15.2% increaseHaylage 16.7% increase
Corn silage 12.6% increaseHaylage 11.0% increase
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BioZyme Incorporated
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AmafermAmaferm Effect across Effect across HaylageHaylageQualitiesQualities -- LigninLignin
AmafermAmaferm Effect across Effect across HaylageHaylageQualitiesQualities -- LigninLigninQualities Qualities LigninLigninQualities Qualities LigninLignin
ControlControl
Amaferm
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Amaferm works equally well on grass and legume forages
Amaferm works equally well on grass and legume forages
Legumes
Grasses
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AmafermAmaferm Effects across Corn Effects across Corn Silage QualitiesSilage Qualities -- DMDM
AmafermAmaferm Effects across Corn Effects across Corn Silage QualitiesSilage Qualities -- DMDMSilage Qualities Silage Qualities DMDMSilage Qualities Silage Qualities DMDM
ControlAmafermAmaferm
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AMAFERM® Formulation and M d li St t i
AMAFERM® Formulation and M d li St t iModeling StrategiesModeling Strategies
•• CPM 3.0 and CNCPS 6.1 CPM 3.0 and CNCPS 6.1 –– Increase Increase KdKd for the CHOfor the CHO--BB33 fraction by 10% for Corn Silagesfraction by 10% for Corn Silages–– Increase Increase KdKd for the CHOfor the CHO--BB33 fraction by 20% for fraction by 20% for HaylagesHaylages33 yy y gy g
•• NRC 2001 NRC 2001 Increase NDF digestibility by 7% for corn silagesIncrease NDF digestibility by 7% for corn silages–– Increase NDF digestibility by 7% for corn silagesIncrease NDF digestibility by 7% for corn silages
–– Increase NDF digestibility by 14% for Increase NDF digestibility by 14% for HaylagesHaylages
D M tt I t kD M tt I t k•• Dry Matter IntakeDry Matter Intake–– Neither model accounts for expected improvement in DMINeither model accounts for expected improvement in DMI–– Predict DMI improvement as 1.0 Predict DMI improvement as 1.0 -- 2.3 lbs 2.3 lbs
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AMAFERM® – Impact on AMAFERM® – Impact on Microbial ProteinMicrobial Protein
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Effect of Effect of AMAFERMAMAFERM®® on on Microbial Efficiency and Microbial Efficiency and
Metabolism in a ContinuousMetabolism in a ContinuousMetabolism in a Continuous Metabolism in a Continuous Culture of Rumen ContentsCulture of Rumen Contents
University of West VirginiaUniversity of West VirginiaBill H Ph DBill Hoover, Ph.D.
Tammy Miller-Webster, M.S.
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Experimental Design:Experimental Design:Th ti lt t i l l t d (• Three continuous culture trials completed (June 07, Sept. 07 & Aug. 08)
• Minimum of three reps / treatment
All di t f t d i t i li t f i d• All diets fermented in triplicate for nine days
• Effluent samples pulled for assay over the last three days
C ti F t ti lt diti• Continuous Fermentation culture conditions were:– Apparatus Scale: 60:1 rumen to fermentation vessel
– 13%/Hour liquid dilution rateq
– 22 hours Solids Retention Time
– 100 grams DM / day
– Feeding frequency 25 grams DM, 4 X daily at 6 hour intervals
– Fermentation temp 390 C
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Diet Analysis (% DM)Diet Analysis (% DM)June ’07 Sept. ’07 Aug. ’08
Forage / Concentrate Ratio 51.7 51.7 59.7Crude Protein 17 6 18 0 17 0Crude Protein 17.6 18.0 17.0Soluble Protein (% CP) 29.0 27.8 42.2NDF 32.3 30.2 32.23 3 30 3ADF 22.1 19.0 20.0NSC 28.5 32.0 29.5Starch 22.5 27.2 24.5Sugar 5.9 4.7 5.0Eth E t t 2 6 3 3 2 5Ether Extract 2.6 3.3 2.5Ash 7.2 6.9 6.5NFC 40 1 41 7 42 0
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NFC 40.1 41.7 42.0
Hoover and Miller, 2007-2008. University of West Virginia, Unpublished
Experiential ResultsExperiential ResultsJ 07 S t b 07 A t 08June 07 September 07 August 08
(Main Effects) Cntl Trt P< %3 Cntl Trt P< %3 Cntl Trt P< %3
By-Pass N (g/day) 1.16 1.06 NS (8.6) 1.24 .91 NS (26.6) 1.31 1.03 .06 (21.3)
Microbial N (g/day) 1.82 1.96 NS 7.7 1.78 2.17 .07 21.9 1.56 1.81 .11 16.0
Total MP 2.98 3.02 NS 1.3 3.02 3.06 NS 2.3 2.87 2.84 NS (1.0)% Microbial Protein4 61 vs 65 59 vs 71 54 vs 64% Improvement5 6.6% 20% 18.5%
Microbial N/Kg DMD1 25.9 28.4 NS 9.7 24.9 30.3 NS 21.7 22.0 26.1 .027 18.6
Microbial N/Kg CHOD2 47.0 51.0 NS 8.5 45.5 53.1 NS 16.7 36.4 44.1 .07 21.0
1) Microbial N produced per Kg dry matter digested
2) Microbial N produced per Kg carbohydrate digested
3) % Improvement between treatments
4) Microbial protein as a percentage of total metabolizable protein
5) Improvement in the percentage of Microbial protein between treatments
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5) Improvement in the percentage of Microbial protein between treatments
University of West Virginia, Unpublished
AMAFERM® – Impact on AMAFERM® – Impact on Rumen pHRumen pH
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Lactate UptakeLactate UptakeppLactate uptake by Megasphaera elsdenii
3.5
43.94*
) (97% Increase)
Control
Amaferm
2
2.5
3
2.01
ity (
nmol
/mg) (97% Increase) Amaferm
0
0.5
1
1.5
Spec
ific
act
iv
0
Control Amaferm
S
* P < 0.05
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BioZyme Incorporated
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Rumen pHRumen pHRumen pHRumen pH
5 856.02
6
6.5
Control
AMAFERM5.85
5.5
6 AMAFERM
5
Control AmafermControl Amaferm
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BioZyme Incorporated
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Rumenocentesis 2-3 h after the AM feeding
Impact on VFA ProductionImpact on VFA Production
Control AMAFERM
Increase in Total Volatile Fatty Acid production.
Total VFA (mM/l) 64.6 67.9A t t l l 2 35 2 49
Control AMAFERM
Acetate pool, moles 2.35 2.49Propionate pool, moles 0.47 0.47Butyrate pool, moles 0.41 0.48Branched chain VFA pool, moles 0.13 0.15Acetate/Propionate (ratio) 4.96 5.3pH 6.34 6.44
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AMAFERM® – Impact on Heat AMAFERM® – Impact on Heat StressStress
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Amaferm Heat Stress PapersAmaferm Heat Stress PapersAmaferm Heat Stress PapersAmaferm Heat Stress Papers1. “Influence of feeding an Aspergillus oryzae culture during Hot Weather on
Performance of Lactating Dairy Cow”, J.T. Huber, G.E. Higginbotham and R. Gomez, 1986 Journal of Dairy Science 69 (supplement 1):188 pg 290
2. “Influence of feeding vitaferm, containing an enzyme-producing culture from Aspergillus oryzae, on performance of lactating cows”, J.T. Huber, G.E. Higginbotham, J of Dairy Science 68 (Suppl 1):30
3. “Influence of feeding Vita Ferm during hot weather on performance of lactating i l d i h d” 1986 K M M J T H b S C J l fcows in a large dairy herd”, 1986, K.M. Marcus, J.T. Huber, S. Cramer, Journal of
Dairy Science 69 (Supplement 1):188 pg 2924. “The Effect of Feeding Aspergillus oryzae culture-vitamin mix on the
performance of lactating dairy cows during periods of heat stress”, M.V. Wallentine N P Johnston D Andrus R Jones J T Huber and G HigginbothamWallentine, N.P. Johnston, D. Andrus, R. Jones, J.T. Huber, and G. Higginbotham. 1986 Journal of Dairy Science 69 (supplement 1):188, pg 294
5. “Influence of Feeding Aspergillus oryzae fermentation extract on the Milk Yields, Eating Patterns, and Body Temperatures of Lactating Cows”, R. A. Gomez-Alarcon J T Huber G E Higginbotham F Wiersma D Ammon and BGomez Alarcon, J.T. Huber, G.E. Higginbotham, F. Wiersma, D. Ammon and B. Taylor, Journal of Animal Science Vol. 69:Number 4, April 1991, pg 1733-1740
6. “Effect of Feeding Amaferm (Aspergillus oryzae extract) to Cows in Early Lactation on Milk Yields and Related Parameters” R. Gomez-Alarcon, F. Wiersma, D. Ammon, G.E. Higginbotham and J.T Huber. J. of Dairy Science
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, , gg y71(suppl 1) P302 1988
Number Lacatation
Milk ProductionMilk ProductionMilk ProductionMilk Productionof Cows Stage Control Amaferm % Diff.
Harris 32 Mid 54.4 57.3 5.3Van Horn 32 Early-Mid 53.9 57.6 6.9Huber 12 Mid 40 7 44 4 9 1Huber 12 Mid 40.7 44.4 9.1
12 Mid 42.7 41.1 <3.7>Wallentine 50 Early 66.2 74.1 12.0
100 Overall 65.1 67.6 3.8Marcus 195 Early-Mid 63.8 67.5 5.9
University Published ResultsMarcus 195 Early Mid 63.8 67.5 5.9Kellems 32 Early 78.3 85.6 9.2
32 Early-Mid 79.4 84.0 5.832 Mid 73.3 76.3 4.2
Kellems 96 Early-Mid 60.6 63.4 4.6Si t 12 E l 77 2 78 1 0 1
4.73% average increase (3.13 lbs)
11.00% increase in early lactationSievert 12 Early 77.2 78.1 0.112 Early 75.9 76.6 0.1
Gomez 24 Mid 49.1 51.0 4.046 Early 85.1 88.9 4.4
Dennigan 40 Early 67.9 67.8 <0.1>
11.00% increase in early lactation
6.40% increase during heat stressDennigan 40 Early 67.9 67.8 0.1
24 Mid 57.7 57.1 <0.1>Baumgard 22 Early 89.7 98.4 9.7
Ave. Average/trial 65.6 68.7
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Average percent increase 4.73%Average percent increase 4.73%
AMAFERM® – Impact on AMAFERM® – Impact on EconomicsEconomics
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The key to increase profits from your forage!
The key to increase profits from your forage!from your forage! from your forage!
Milk/Feed Wt Milk Fat Milk SCC Milk lbsMilk/Feed Wt Loss
Milk Fat Milk Protein
SCC Milk lbs
C t l 1 77 122 4 22 3 39 613 76 9Control 1.77 122 4.22 3.39 613 76.9
Amaferm 1.89 106 4.50 3.56 443 82.9
6.7% -16 lbs 6.6% 5.0% -170 +6 lbs
6 lbs additional milk @ $.15 = $.904.5 : 1 Return
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BioZyme Incorporated
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Spruce HavenSpruce HavenField DemonstrationField Demonstration
Spruce HavenSpruce HavenField DemonstrationField DemonstrationField Demonstration Field Demonstration Field Demonstration Field Demonstration
• Trial setup– Two pens 68 cows/pen – 41 cows per treatment (balanced for milk)– 100 DIM– Two phases
• Phase 1 (7wks) Control and Amaferm• Phase 2 (5 wks) Control and AmafermPhase 2 (5 wks) Control and Amaferm
– 1 lb of liquid molasses (ration adjusted, iso energy and protein)
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Amaferm Field Demonstration Amaferm Field Demonstration Amaferm Field Demonstration Amaferm Field Demonstration Control/Amaferm Amaferm/Molasses
Dry Matter 47.9 48.2Crude Protein 17.5 17.3RDP 53.6 53.8Soluble 34.9 36.3RUP 46 4 46 2RUP 46.4 46.2NEL (Mcal/lb) 0.78 0.78Forage NDF % 78.3 78.3Sugar, % 5.9 7.1Starch, % 23.6 23.2Soluble fiber, % 7.08 7.1, %Total fat, % 5.7 5.6Yeast* + +
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* Celmanax
Phase 1Phase 1Phase 1Phase 1
Control
AMAFERMAMAFERM
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Amaferm Field DemonstrationAmaferm Field DemonstrationPhase 1Phase 1
Amaferm Field DemonstrationAmaferm Field DemonstrationPhase 1Phase 1Phase 1 Phase 1 Phase 1 Phase 1
Control Amaferm ChangegDMI (lbs) 57.3 55.4 -1.9Milk (lbs) 86.2 88.0* 1.8Milk (lbs) 86.2 88.0 1.8F:E 1.50 1.59 6.0%
* P = 0.04
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Economics Phase 1 Economics Phase 1 Economics Phase 1 Economics Phase 1
Control AmafermFeed/ton DM $192.88 $200.10Daily feed cost $ 5.53 $ 5.54yMilk/cwt $ 15.00 $ 15.00Daily milk value $ 12.93 $ 13.20y $ $IOFC $ 7.40 $ 7.66Advantage $ 26*Advantage $ .26
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* Return over the cost of Amaferm
Phase 2Phase 2Phase 2Phase 2
Control
AMAFERMAMAFERM
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Amaferm Field DemonstrationAmaferm Field DemonstrationPhase 2Phase 2
Amaferm Field DemonstrationAmaferm Field DemonstrationPhase 2Phase 2Phase 2Phase 2Phase 2Phase 2
Control Amaferm ChangeDMI (lbs) 54.9 56.3 1.4Milk (lbs) 76.5 81.3* 4.8F:E 1.39 1.44 3.6%
* P = 0.001
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Economic Phase 2Economic Phase 2Economic Phase 2Economic Phase 2
Control AmafermFeed/ton DM $192.88 $203.17Daily feed cost $ 5.29 $ 5.69Milk/cwt $ 15.00 $ 15.00Daily milk $ 11 48 $ 12 20Daily milk $ 11.48 $ 12.20IOFC $ 6.18 $ 6.51Ad $ 33*Advantage $ .33*
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* Return over the cost of Amaferm
Number Lacatation
Milk ProductionMilk ProductionMilk ProductionMilk Productionof Cows Stage Control Amaferm % Diff.
Harris 32 Mid 54.4 57.3 5.3Van Horn 32 Early-Mid 53.9 57.6 6.9Huber 12 Mid 40 7 44 4 9 1Huber 12 Mid 40.7 44.4 9.1
12 Mid 42.7 41.1 <3.7>Wallentine 50 Early 66.2 74.1 12.0
100 Overall 65.1 67.6 3.8Marcus 195 Early-Mid 63.8 67.5 5.9Marcus 195 Early Mid 63.8 67.5 5.9Kellems 32 Early 78.3 85.6 9.2
32 Early-Mid 79.4 84.0 5.832 Mid 73.3 76.3 4.2
Kellems 96 Early-Mid 60.6 63.4 4.6Si t 12 E l 77 2 78 1 0 1Sievert 12 Early 77.2 78.1 0.1
12 Early 75.9 76.6 0.1Gomez 24 Mid 49.1 51.0 4.0
46 Early 85.1 88.9 4.4Dennigan 40 Early 67.9 67.8 <0.1>Dennigan 40 Early 67.9 67.8 0.1
24 Mid 57.7 57.1 <0.1>Baumgard 22 Early 89.7 98.4 9.7
Ave. Average/trial 65.6 68.7
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Average percent increase 4.73%Average percent increase 4.73%
Number Lacatation
Milk ProductionMilk ProductionMilk ProductionMilk Productionof Cows Stage Control Amaferm % Diff.
Harris 32 Mid 54.4 57.3 5.3Van Horn 32 Early-Mid 53.9 57.6 6.9Huber 12 Mid 40 7 44 4 9 1Huber 12 Mid 40.7 44.4 9.1
12 Mid 42.7 41.1 <3.7>Wallentine 50 Early 66.2 74.1 12.0
100 Overall 65.1 67.6 3.8Marcus 195 Early-Mid 63.8 67.5 5.9
University Published ResultsMarcus 195 Early Mid 63.8 67.5 5.9Kellems 32 Early 78.3 85.6 9.2
32 Early-Mid 79.4 84.0 5.832 Mid 73.3 76.3 4.2
Kellems 96 Early-Mid 60.6 63.4 4.6Si t 12 E l 77 2 78 1 0 1
4.73% average increase (3.13 lbs)
11.00% increase in early lactationSievert 12 Early 77.2 78.1 0.112 Early 75.9 76.6 0.1
Gomez 24 Mid 49.1 51.0 4.046 Early 85.1 88.9 4.4
Dennigan 40 Early 67.9 67.8 <0.1>
11.00% increase in early lactation
6.40% increase during heat stressDennigan 40 Early 67.9 67.8 0.1
24 Mid 57.7 57.1 <0.1>Baumgard 22 Early 89.7 98.4 9.7
Ave. Average/trial 65.6 68.7
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Average percent increase 4.73%Average percent increase 4.73%
AmafermAmaferm DoseDoseAmafermAmaferm DoseDose
• Feeding Recommendations– Transition 10-15 grams
– Cows (Lactating/Non-lactating) 5-10 grams– Replacement Heifers 2- 3 gramsReplacement Heifers 2 3 grams
– Calves (under 400 lbs) 1- 2 grams
* Recommended rates are based on producers ability to phase feed.
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When When AmafermAmafermWhen When AmafermAmaferm• Transition• Early lactationy• Higher forage diets• Herds looking for increased component yield • During periods of Heat stress
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“The Fiber Digestion Additive”“The Fiber Digestion Additive”“The Fiber Digestion Additive”“The Fiber Digestion Additive”
What will your clients choose?What will your clients choose?
ThisThis ThisThis
OrOr
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AMAFERMAMAFERM®® –– University University AMAFERMAMAFERM®® –– University University StudiesStudiesStudiesStudies
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University of ArizonaUniversity of ArizonaUniversity of ArizonaResearcher: Lance Baumgard, Ph.D.
University of ArizonaResearcher: Lance Baumgard, Ph.D.
Effect of AMAFERM®Effect of AMAFERM®
on Production & Metabolic Parameters in Transition Cows
on Production & Metabolic Parameters in Transition Cows
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St. Joseph, MO. USA
Experimental DesignExperimental Design• 33 cows (22 multiparous & 11 primiparous) were assigned to either
control diet, or control plus AMAFERM (Aspergillus oryzae fermentation extract product)fermentation extract product)
• Study Term: 21 days prepartum through 60 days postpartum
• Cows were fed 2X daily via Calan® gate systemCows were fed 2X daily via Calan gate system
• Cows milked 2X, individual milk weights collected daily.
• Body weights collected weekly during the postpartum periodBody weights collected weekly during the postpartum period
• Statistics: SAS, PROC MIXED procedures
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Baumgard et al., 2003. JDS Vol 87 Suppl. 1 pg 609
Diet % DMDiet % DMPostpartum Prepartum
Alfalfa Hay 59.0% 62.1%Whole Cotton Seed 6.0% 3.5%Citrus Pulp 7.8% 11.8%Stem Flaked Corn 15.7% 19.0%Amino Plus 2.2%Energy ll 1.7%Molasses 4 2%Molasses 4.2%Mineral 3.4% 3.6%
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Baumgard et al., 2003. JDS Vol 87 Suppl. 1 pg 609
Study ResultsStudy ResultsC t lC t l A fA f P<P<ControlControl AmafermAmaferm P<P<
Max. body wt loss (all cows) 122 106 NSWeek of BW Nadir (all cows) 6.2 5.0 .06Week of BW Nadir (all cows) 6.2 5.0 .06NEFA (µeq/L) (all cows) 669 573 .09DMI (lbs) (prepartum) 31.3 33.0 NS
(postpartum) 43.4 43.9 NSMilk Production (lbs) (all cows) 76.9 82.9 .05
(Multiparous) 89 7 98 4 001(Multiparous) 89.7 98.4 .0013.5% FCM (all cows) 85.9 96.3Milk / Feed efficiency (all cows) 1.77 1.89Milk fat (%) 4.22 4.50 .14Milk protein (%) 3.39 3.56 NSSCC 613 443 NS
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Source: Baumgard et al., 2003. JDS Vol 87 Suppl. 1 pg 609
SCC 613 443 NS
Milk Response of Dairy Cattle Milk Response of Dairy Cattle ithith AMAFERMAMAFERM®with with AMAFERMAMAFERM®
Alex Bach Ph DAlex Bach, Ph.D.IRTA-Dairy Systems
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Experimental DesignExperimental Design• 20 lactating cows (10 primiparous, 10 multiparous) were assigned
to one of 2 treatments (Control or AMAFERM®)
A ill f t ti t t d t– Aspergillus oryzae fermentation extract product
• Fed once daily via a total mixed ration
• Cows were grouped and acclimated to the basal diet 30 day prior• Cows were grouped and acclimated to the basal diet 30 day prior to the start of AMAFERM supplementation.
• Cows were blocked by both DIM (post peak) and level of production.
• Cows received AMAFERM for a period of 90 days.
Milk production was recorded daily and milk composition monthly• Milk production was recorded daily and milk composition monthly.
BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA Bach et. al. 2003 ITRA, Unpublished
Basal Diet Analysis Basal Diet Analysis Ingredients Composition
(% of DM)
Rye Grass Silage 17.7
Nutrient Composition(% of DM)
Crude Protein 17.3Wheat Silage 14.5Alfalfa Silage 10.6Citrus Pulp 7.7
Ether Extract 4.2NDF 33.3ADF 22.0
42.8%42.8%
Corn Gluten Feed 4.8Whole Cottonseed .2Corn Grain 24.4
NFC 37.7NEL .695
12.7%12.7%
Barley Grain 2.0Soybean Meal 11.1Lupine Meal 4.0Vit. / Min .9Salt .3
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Bach et. al. 2003 ITRA, Unpublished
Experimental Results Experimental Results (Study Average of 253 DIM)(Study Average of 253 DIM)(Study Average of 253 DIM)(Study Average of 253 DIM)
Performance of dairy cattle as influenced by AMAFERM
Treatment Milk Fat FCM (3.5%) Protein(lbs) (%) (lbs) (%)
Control 57.3a 3.46 56.9a 3.53AMAFERM 62.7b 3.70 64.7b 3.44Difference 5.4 .24 7.8 .09% I t 9 4% 6 9% 13 7% 2 5%
All means are the sum of the square means Different subscripts indicate significant differences (P<.05)
% Improvement 9.4% 6.9% 13.7% -2.5%
• No health problems documented within the studyNo health problems documented within the study
• Cows supplemented with AMAFERM demonstrated a lower milk drop as days in milk increased
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA Bach et. al. 2003 ITRA, Unpublished
AMAFERM® – Field StudiesAMAFERM® – Field StudiesAMAFERM – Field StudiesAMAFERM – Field Studies
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St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERM® Dairy Tech Update
Measuring the ECM Effect of a 5 gram AMAFERMMeasuring the ECM Effect of a 5 gram AMAFERM Feeding Rate in a Large, Production Dairy:
• Facilities and Management:– A 900 cow 2X free stall dairy in ID feeding a one group TMR.
• Trial Design:– The 4 breeding pens were randomly assigned to one of two treatments (two pens per treatment);The 4 breeding pens were randomly assigned to one of two treatments (two pens per treatment);
Control & AMAFERM with approximately 100 cows per pen.
– All rations were identically formulated with the exception of the addition of AMAFERM to the treatment pens
– October, 2008 test day milk weights were used to establish initial base line production for all cows. No R mensin® Posilac® or DFMs ere sed ithin the e al ationRumensin® , Posilac®, or DFMs were used within the evaluation
– AMAFERM supplementation was initiated 12 days prior to November test date and continued through the December test date to determine relative changes in ECM (energy corrected milk) on the two test days.
– Pen inventories were completed bi-weekly and immediately prior to individual test days. p y y p y
– Cows were eliminated from the study if they moved from their original pen, exhibited estrus on day of test or had a significant increase in SCC status
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
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BioZyme Incorporated
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Table 1. Rations:
Ingredients Oct. Nov. Dec. Analysis Oct. Nov. Dec.Ingredients Oct. Nov. Dec. Analysis Oct. Nov. Dec.
Alfalfa Hay 10.42 16.42 12.77 DM (%) 55.29 63.4 59.6
Corn Silage 16.55 9.16 13.73 CP (%) 17.34 18.76 18.25
Wheat Straw .98 .92 .92 Sol. Protein (%) 36.25 36.08 35.76
Canola 2.24 4.12 3.7 RDP (%) 10.67 11.43 10.99
SBM 1 11 2 03 1 83 NDF (%) 31 34 31 76 33 19SBM 1.11 2.03 1.83 NDF (%) 31.34 31.76 33.19
Gluten Feed 5.73 5.4 5.4 NFC (%) 41.18 40.77 39.63
Wheat Midds 5.73 1.82 3.73 Starch (%) 25.72 24.0 24.0
Blood Meal .96 .7 1.0 Sugar (%) 5.11 6.11 5.75
Corn w/ 5% fat 8.33 11.71 9.8 EE (%) 4.39 4.41 4.28
Minerals 1.06 .99 .99
Total DM 53.10 53.27 53.87
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Table 2. Production results measured over three test periods:
Lactation 1 Lactation 2 Lactation 3+
Parameters Control Trt. Control Trt. Control Trt.
Cow #’s1 35 18 29 53 55 49
Oct DIM 159 89 136 152 220 199Oct. DIM 159 89 136 152 220 199
Oct. ECM 77.5 67.0 85.7 81.3 87.5 89.7
Nov. ECM 71.5 66.2 76.2 76.3 80.4 85.5
Dec. ECM 78.0 69.3 77.8 77.0 76.0 84.9
ECM Change2
Oct. - Nov-6.0 -.8 -9.5 -5.0 -7.1 -4.2
AMAFERM Advantage 5.20 4.50 2.90
ECM ChangeOct. – Dec.
.05 2.3 -7.9 -4.3 -11.5 -4.8
AMAFERM Advantage 1.80 3.60 6.70
Avg. AMAFERM Response
3.5 lbs 4.05 lbs 4.8 lbs
1.1. Cows that started and finished the study within their allocated penCows that started and finished the study within their allocated pen2.2. Nov. ECM milk represents cows that were on treatment for 12 days prior to test dayNov. ECM milk represents cows that were on treatment for 12 days prior to test day
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
AMAFERM® Dairy Tech Update
Measuring the ECM Effect of a 5 gram AMAFERMMeasuring the ECM Effect of a 5 gram AMAFERMFeeding Rate in a Dry Lot Dairy:
• Facilities and Management:– A 10,000 cow 2X free stall dairy in ID feeding a one group TMR.
• Trial Design:Th 4 d i d l i d f ( )– The 4 production pens were randomly assigned to one of two treatments (two pens per treatment); Control & AMAFERM with approximately 300 cows per pen.
– All rations were identically formulated with the exception of the addition of AMAFERM to the treatment pens. Rumensin® fed at 300 mg / day
– December 2008 test day milk weights were used to establish initial base line production for all cowsDecember 2008 test day milk weights were used to establish initial base line production for all cows
– AMAFERM supplementation was initiated 21 days prior to January test date and continued through the February test date to determine relative changes in ECM (energy corrected milk) on the two test days.
– Pen inventories were completed bi-weekly and immediately prior to individual test days.
– Cows were eliminated from the study if they moved from their original pen exhibited estrus on day ofCows were eliminated from the study if they moved from their original pen, exhibited estrus on day of test or had a significant increase in SCC status
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Table 1. Rations:
Ingredients DEC. JAN. FEB. Analysis DEC. JAN. FEB.Ingredients DEC. JAN. FEB. Analysis DEC. JAN. FEB.Alfalfa Hay 15.1 15.0 18.0 DM (%) 51.57 51.1 50.1
Corn Silage 9.9 10.4 10.4 CP (%) 16.8 17.4 17.5
Alfalfa Haylage 2.48 2.6 2.6 Sol. Protein (%) 39.9 44 44
i i 4 1 2 1 (%) 10 62 11 4 11 63Beet Tailings 4.7 1.5 2.1 RDP (%) 10.62 11.45 11.63
Flaked Corn 9.4 7.8 7.35 NDF (%) 31.38 30.9 30.6
Canola Meal 4.1 4.0 3.1 NFC (%) 41.23 41.65 41.0
DDG 1.8 1.72 1.76 Starch (%) 21.92 20.12 20.0
B P l 1 0 0 0 S (%) 6 73 7 86 7 96Beet Pulp 1.0 0 0 Sugar (%) 6.73 7.86 7.96
Cotton Hull Pellet 1.0 0 0 EE (%) 4.66 4.58 4.56
Mill Run 1 0 0
Corn Steep 1.3 1.35 1.35
Wh 0 1 35 1 35Whey 0 1.35 1.35
High Fat Pellets 2.5 1.88 1.87
Minerals .85 .88 .96
Total DM 55.3 52.3 52.2
BioZyme Incorporated
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BioZyme Incorporated
St. Joseph, MO. USA
BioZyme Incorporated
St. Joseph, MO., USA
BioZyme Incorporated
St. Joseph, MO. USA
Table 2. Production results measured over three test periods:
Lactation 1 Lactation >1
Parameters Control3 Trt. Control3 Trt.
Cow #’s1 142 200 30 17
Dec DIM 188 176 116 108Dec. DIM 188 176 116 108
Dec. ECM 77.5 79.3 98.4 97.9
Jan. ECM 81.8 89.5 89.1 95.9
Feb. ECM 74.4 80.2 80.5 83.7
ECM Change2
Dec. – Jan.+4.3a +10.2b -9.3 -2.0
AMAFERM Advantage +5.75 +7.24
ECM Ch 2 3 1a + 8b 17 9 14 2ECM Change2
Dec. – Feb.-3.1a +.8b -17.9 -14.2
AMAFERM Advantage +3.9 +3.7
Avg. AMAFERM Response 4.825 lbs 5.47 lbs
1.1. Cows that started and finished the study within their allocated penCows that started and finished the study within their allocated pen2.2. Means in the same row with different superscripts differ (p<.01)Means in the same row with different superscripts differ (p<.01)3.3. Control pens had a disproportion number of animals over 250 DIM (157 Control pens had a disproportion number of animals over 250 DIM (157 --14). All animals over 250 14). All animals over 250
DIM DIM were excluded from this analysis.were excluded from this analysis.
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BioZyme Incorporated
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were excluded from this analysis. were excluded from this analysis.