Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Influence of whisking-related activity in the motor cortex on sensory processing in the somatosensory cortex of rodents
Werner Kilb
Institut für Physiologie und Pathophysiologie
Universitätsmedizin Mainz
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Background
Gentet et al. (2012)Nat Neurosci:15:607-12
Voltage-sensitive dye
Whole-cell recording
Petersen (2007) Neuron 56:339
Diamond et al (2008) Nature Reviews 9:601
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
BackgroundVoltage-sensitive dye
Whole-cell recording
Petersen (2007) Neuron 56:339
Diamond et al (2008) Nature Reviews 9:601
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Background
Diamond et al (2008) Nature Reviews 9:601
Ferezou et al. (2007) Neuron 56:907
Mao et al. (2011) Neuron 72:111
Matyas et al. (2010) Science 330:1240
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Central question
Voltage-sensitive dye
Whole-cell recording
Petersen (2007) Neuron 56:339
wM1
Modified from Diamond et al (2008)
How does neuronal activity in the whisker-related motor cortex influence information processing in the somatosensory (barrel) cortex?
b) What is the Contextual control?
How does the somatosensory cortex process whisker-related informationin the context of
whisking-related activity in the motor cortex?
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Short overview on planed experiments
wM1
Modified from Diamond et al (2008)
1.) In-vivo recordings
Stimulatory context
- MEA-recordings (FP, MUA, single-units) (simultan. in S1 and wM1)
- inter- and transcolumnar information flow/processing
- repetitive spike patterns
Interference with signal processingSilencing (Lidocain) at different levels of information processingBlocking motor outputs (d-Tubocur./Botulinumtoxin)Optogenetic activation/silencing
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Short overview on planed experiments.
wM1
Modified from Diamond et al (2008)
1.) In-vivo recordings - MEA-recordings (FP, MUA, single-units) (simultan. in S1 and wM1)
- inter- and transcolumnar information flow/processing
- repetitive spike patterns
2.) In-vitro recordings - motosensori slices and cortical slab preperations
- influence of motorcortex activation on neuronal properties (Em, PSCs, APs, Resonance) in different neurons of the barrel field
- effect of serotonin on neurons in the somatosensory cortex
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Preliminary workSimultaneous recordings of sensorimotor interactions
FP-recording
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Preliminary workMethods to define/analyze information processing?
Werner Kilb Preparatory meeting SFB 07. Sept. 2012
Influence between motor and sensory cortex during whisking
Integration into the CRC „Contextual control“Direct link to two other projects investigating „active sensing“
- Torfi Sigurdsson „Neural Circuits Underlying Corollary Discharge in Mouse Auditory Cortex“
- Prof. Kössl „Context-dependence of cortical auditory time perception”
May serve to give conceptual support (e.g. unit-activity underlying oscillatory events) to the (human) study regarding “spectrotemporal dynamics of feedforward and feedback processing in sensorimotor transformations“ (Prof. Kell).
The analysis of our datasets will benefit from the expertise provided within this consortium applying for this CRC. In particular I plan collaborations with
Prof. Wibral (Model free measures of information flow, dynamic causal modeling)
Dr. G. Schneider (Information capacity of a single oscillatory cycle)