The Evolution of Cytochrome P450 in Animals David R. Nelson University of Tennessee Dept. of Molecular Sciences Center of Excellence in Genomics and Bioinformatics Drug Metabolism in humans CYP3A4/3A5, CYP2D6, CYP2C9 ~75% + CYP2C19, CYP2E1, CYP1A2, CYP2A % Metazoan Genomes Sampler Halocynthia roretzi adult and larva Suberites domuncula plus hermit crableech (annelid worm) Chordate Cnidarian Porifera (sponges) UPGMA tree of Hydra P450s 2 Clan 3 Clan 4 Clan Cortisol 11-Deoxdycortisol 11-Deoxycorticosterone Corticosterone Aldosterone 11A1 CYP17 CYP19 11B1 CYP21 11B1 11B2CYP17 UPGMA tree of Amphioxus CYPs (not mito clan) CYP51 CYP7/39 Clan CYP20 CYP26 Clan CYP4 Clan CYP3 Clan CYP19 CYP2 Clan CYP27A1 CYP27B1 active form CYP24 inactive CYP2R1 Neighbor-joining tree of amphioxus mito clan P450s CYP11 CYP24 CYP27