Genetic Modification of Eukaryotes, Part 1

I. Transgenic Animals (GMO’s)a. What are GMO’s?

b. A Brief History of GMO animalsi. Restriction Enzymes

ii. Recombinant DNA

iii. Recombinant Bacteria

iv. The Asilomar Conference on Recombinant DNA1. Recommendations

2. Experimental Design

3. Prohibited Experiments

v. Recombinant Animal Cells

vi. Transgenic Animals (https://www.nature.com/scitable/knowledge/library/transgenic-animals-in-agriculture-105646080)

1. Common techniques used

2. Applications in Agriculturea. Enhanced nutrition

b. Enhancing milk

c. Enhancing growth rates and carcass composition

d. Enhanced animal welfare

e. Improving reproductive performance

f. Improving hair and fiber

g. Pitfalls and risks

h. Aquabounty Salmon

3. GM (Oxitec) Mosquitoeshttp://www.the-scientist.com/?articles.view/articleNo/50636/title/GM-Mosquitoes-Closer-to-Release-in-U-S-/&utm_campaign=NEWSLETTER_TS_The-Scientist-Daily_2016&utm_source=hs_email&utm_medium=email&utm_content=57389453&_hsenc=p2ANqtz-84xxaNVTqZEBU9ujNCq8yc-16jc5NhzYlcycD5RP0KGyRQkDE4w60P0evP1_eHAleK_ednPYskpAqcBQX33_mMHYq-TQ&_hsmi=57389453

II. Gene Therapy and Leber Congenital Amaurosis

a. The Gene

b. The Mouse

c. The Vector

d. The dogs

e. Human studies