© 2007 Nature Publishing Group
Supplemental figures for
All-optical anatomical coregistration for small animal molecular imaging using dynamic contrast.
Elizabeth M. C. Hillman
1 and Anna Moore
2
1Laboratory for Functional Optical Imaging, Department of Biomedical Engineering, Columbia University, 1210
Amsterdam Avenue, New York, NY 10027. 1-212-854-2788, [email protected].
2Molecular Imaging Laboratory, MGH/MIT/HMS Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts
General Hospital, Building 149, 13th
Street, Charlestown, MA 02129
Figure S1. Raw timecourse images of indocyanine green and dextran texas red following simultaneous injection
Figure S2. In-vivo anatomical maps derived using PCA of a 20 second image series following ICG injection
Figure S3. In-vivo optical spatiotemporal separation of nine ‘organs’ using basis timecourses (ICG only).
Figure S4. Comparison of DFMI anatomical image with digital anatomical atlas.
© 2007 Nature Publishing Group
Figure S1. Raw timecourse images of indocyanine green and dextran texas red following
simultaneous injection. Left: Raw data series of ICG and Texas red from injection to 2 minutes post-
injection. Mouse is prone. Each image is normalized to its own maximum. Right: Timecourses of
selected regions are shown for nominally identified organs. Top row shows absolute timecourse,
bottom row shows time-course divided by the mean time-course. Note high initial fluorescence in large
intestine at texas-red wavelengths (corresponding to food autofluorescence). Note the rapid transit of
ICG in the lungs (as the direct route from the tail vein to the right ventricle to the pulmonary artery).
© 2007 Nature Publishing Group
Figure S2. In-vivo anatomical maps derived using PCA of an image series following ICG
injection (same as figure 2 in main text, but for first 20 seconds of mouse A). Left: maps of the 2nd
, 3rd
and 4th
temporal principal components of images acquired during the first 20 seconds after ICG
injection (color-coded red, green and blue). The mouse is in a prone position. Right: The
corresponding PCA basis timecourses of the 1st – 4
th components of the image series. Each identified
organ has a different red-green-blue combination, and hence each has its own distinctive timecourse.
The spikes in the 4th component correspond to breathing-related motion. Labeled organs were
identified by comparison with post-mortem dissection and general anatomy.
© 2007 Nature Publishing Group
Figure S3. In-vivo optical spatiotemporal separation of nine ‘organs’ using basis timecourses
(ICG only). The individual spatial maps resulting from a non-negative least-squares fit of 9 basis
timecourses to a 26 minute long image series (these are the individual elements of the image
composition shown in figure 3 of the main text). Fit results are shaded cyan and overlaid onto a feint
bright-field image. In most cases, localization to one place is achieved. Additional structure indicates
that these regions also have similar dynamic behavior to the primary region.
© 2007 Nature Publishing Group
Figure S4. Comparison of DFMI anatomical image with digital anatomical atlas. Top: image from
figure 3 in main test. Bottom: Digimouse anatomical atlas http://neuroimage.usc.edu/Digimouse.html
red = liver, blue = spleen, purple = kidney, green = heart, pink = brain, cyan = lungs (pale blue =
bladder, yellow = testes [digimouse is male, dynamouse is female]).