Molecular Constellations 2014Optics beyond the Abbe Limit
C. Cremer
Institute of Molecular Biology (IMB), Mainz/DEInstitute for Pharmacy & Molecular Biotechnology (IPMB)/Kirchhoff-Institute for
Physics (KIP), University Heidelberg
For more information see www.imb-mainz.de
Letiz
ia M
anci
no
Heidelberg Mainz
Molecular Constellations in Mouse PachyteneChromosomes
K. Prakash, M. Borsos, S. Redl, H.-K. Lee, K. Tachibana-Konwalski, R. Ketting, C.Cremer, U. Birk
Conventional Microscopy Localization Microscopy (SPDM)
500 nm
Conventional Structured Illumination Localization microscopy
Structured illumination microscopy in human retinadiagnostics:profiles of particles smaller than 500 nm
S. R
ossb
erge
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Br.
J. O
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. 97
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518
–52
3.
Abstract. We survey the history of resolution enhancement techniquesin microscopy and their impact on current research in biomedicine…we present the historical development of various techniques thatsubstantially enhance the optical resolution of the light microscope…
pp 281–344.
3 point-like objects in x,y plane withnext neigbour distances 50 nm
a) Labelling with same spectralsignature
b) Labeling with different uniquespectral signatures B,G,R
Computation (scalar Theory):
NA = 1.4, λexc = 488 nm
Optical Isolation by the
“Supernova“ Mode: Induction of
stochastically distributed short
flashes of light emission by
individual moleculesCremer et al. 1996, 1999, 2010, 2011
Principle of Spectrally Assigned Localization Microscopy (SPDM): Optical Isolation of Diffraction Images of individual Molecules: Determination of Gravity Centers
Publications Cremer Lab 2013
G. Best, R. Amberger, C. Cremer (2013) Super-Resolution Microscopy:Interference and Pattern Techniques. In: Fluorescence Microscopy: FromPrinciples to Biological Applications (Kubitscheck U,Ed.), pp. 345 – 374. Wiley-Blackwell.
C. Cremer, B.R. Masters (2013) Resolution enhancement techniques inmicroscopy. Eur. Phys. J. H, Eur. Phys. J. H 38: 281–344.
S. Rossberger, T. Ach, G. Best, C. Cremer, R. Heintzmann, S. Dithmar (2013)High resolution imaging of autofluorescent particles within drusen usingstructured illumination microscopy, British Journal of Ophthalmology 97: 518 –523.
S. Rossberger, G. Best, D. Baddeley, R. Heintzmann, U. Birk, S. Dithmar, C.Cremer (2013) Combination of structured illumination and single moleculelocalization microscopy in one setup, J. Opt. 15, doi:10.1088/2040-8978/15/9/094003.