• Background
• 1st , 2nd and 3rd FAO-APHCA/OIE Regional Workshop on
Brucellosis Diagnosis and Control with an Emphasis on Brucella melitensis (in collaboration with DLD)
• The 4th FAO-APHCA/OIE/DLD Regional Workshop on
Brucellosis Diagnosis and Control in Asia-Pacific Region
-Proficiency Test and Ways Forward-
Chiang Mai, 19-21 March 2014
• Brucellosis OIE Twinning Laboratory ProgrammeFrance-Thailand
• The 1st and 2nd Asia-Pacific Bovine Brucellosis
Proficiency Test (2013, 2014)
• Outcome and ways forward
The Minutes of 65th Executive Committee Meeting and the 29th
Session of APHCA (25 September 2005)
Thailand - Raised the re-appearance of Brucella melitensis
and its threat
- Requested APHCA to organise a relevant workshop
“ ……. Supported by delegates…….”
i) Brucella melitensis in goats –
diagnosis, vaccination and
other trade related
technical protocols
by B. Garin-Bastuji, c/o OIE;
ii) Special presentation on “OIE pathway for BSE-free status declaration”
by Y. Oketani, OIE-Tokyo Office; and
iii) General discussion on disease issues in goats
Report of the 30th APHCA Session (Luang Prabang, Lao PDR)
Wednesday, 25 October 2006 09 00 – 10 00 Review of day 1 – country priorities for action(s)
10 15 – 12 30 APHCA-OIE Regional workshop on goats (chaired by C. Leowijuk)
Minutes of the 31st Session of APHCA
October 29th -November 1st 2007 , Chiang Mai, Thailand
Future workplan/activities: 2008 period
• Continued activities from 2007
• New Initiatives: 1/4 topics …. Proposed
“Diagnosis and control of Brucella melitensis and Bluetongue”
1st FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis
Chiang Mai and Lampang, 20-23 October 2008
• 17 member countries• Lectures and lab practiced on serological tests• National labs: keep same SOP according to OIE standard
1st FAO-APHCA/OIE Regional Workshop
• Member countries: requested
- Follow up workshop/training
- Continue capacity building of national veterinary services
2nd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis
Khon Kaen, 8-11 June 2009
• 16 member countries
• Lecture and lab practiced – SOP (based on OIE Manual)
• RBT antigens were provided
Requested:
- Quality assurance including standardisation
- Strengthening of Regional collaboration on diagnosis and control of brucellosis
Recommendation for OIE Twinning Programme
2nd FAO-APHCA/OIE Regional Workshop
3rd FAO-APHCA/OIE Regional Workshop on Brucellosis Diagnosis and Control with Emphasis on Brucella melitensis
Sukhothai and Phitsanulok, 21-25 November 2010
• 17 member countries
• Lecture and lab practiced serological tests and reagents control
• Demonstration: Brucella spp. isolation, identification and typing (BSL-3)
Observation and suggestion:
• The member countries
- utilized the knowledge and techniques
- improved/strengthened their laboratory diagnosis capacity
• Under the OIE Twinning Programme
- the participants requested for support on Brucellosis proficiency testing to member countries in the region
3rd FAO-APHCA/OIE Regional Workshop
OIE Reference Laboratory Twinning Programme:
Brucellosis, France-Thailand
• Birth of collaboration………
- 1st FAO-APHCA/OIE Regional Workshop
- 2nd FAO-APHCA/OIE Regional Workshop
• The Brucellosis OIE Twinning Program France-Thailand (ANSES & NIAH-DLD)
- OIE expert: Dr. Bruno Garin-Bastuji, ANSES
- Three-year project: 2010-2013
OIE Reference Laboratory Twinning Programme: Brucellosis
• Brucellosis twinning programme was completed on December 31, 2013…
but collaboration still goes on………..
OIE Reference Laboratory Twinning Programme: Brucellosis
Thailand-Japan Joint Conference on Animal Health 2012
Prince Mahidol Award Conference, 28-31 Jan 2013, Bangkok
OIE Reference Laboratory Twinning Programme: Brucellosis
• NIAH and RVRDCs – poster presentations: brucellosis laboratory surveillance and Twinning for 6 posters
(Thailand-Japan Joint Conference on Animal Health 2012)
OIE Reference Laboratory Twinning Programme: Brucellosis
Preparation: The 1st Asia-Pacific Bovine Brucellosis Proficiency Test 2013
• Under OIE Twinning programme & FAO-APHCA and supervision by Dr. Bruno Garin-Bastuji
• Panels of sera preparation: July 2013
• Distribution
• Report the results in 2014
The 4th FAO-APHCA/OIE/DLD Regional Workshop on Brucellosis Diagnosis and Control in Asia-Pacific Region
-Proficiency Test and Ways Forward-Chiang Mai, 19-21 March 2014
• Review and update on brucellosis
• Quality management in veterinary testing lab
• International shipment
• PT 2013 results
• Distributed PT 2014
Workshop proposed:
Share information for strengthening the regional networking
FAO-APHCA and OIE RRAP should continue providing collaborative support to strengthen Brucellosis diagnostic capacity building
OIE/FAO Ref Lab should continue to provide technical support
Possible ….. Refresh/update hands-on WS on Brucellosis Diagnosis
(upon request)
• Participants 34 labs (results 33 Labs)
• Asia-pacific countries 18 countries, results returned 17 labs
• Regional VRDCs-NIAH 8 labs
• Government enterprise 1 lab
• Universities 4 labs
• Private companies 2 labs
• ANSES
Results: The 1st Asia-Pacific Bovine Brucellosis Proficiency Test 2013
The 1st and 2nd Asia-Pacific Bovine Brucellosis Proficiency Test (2013, 2014)
Outcome and ways forward…………
Animal Health Laboratories
- NIAH, Bangkok
- OIE Regional Reference Laboratory for FMD
- Veterinary Biological Assay Center /ASEAN Assay Lab
- 8 Regional Veterinary Research and Development Centers (7+1) Songkhla
Laboratory Quality assurance: NIAH & RVRDCs
Laboratory Quality assurance: NIAH & RVRDCs
- Serological test for Brucellosis was accredited: RBT and CFT (NIAH and 7 RVRDCs)
ISO 9001:2008
Brucellosis Research collaboration
• QIA-Korea and NIAH-DLD-Thailand: 2014-2016
“The molecular epidemiological analysis of Brucella species from a variety of animals and application on diagnostics for Brucella canisin Thailand”
• NIAH-Japan and NIAH-DLD-Thailand: June 2014-March 2017“Development of Milk-ELISA kit for detection of antibody against Brucella sp.”
@ Training @ Annual Meeting of Brucellosis Laboratories@ Further study: 1 MSc and 1 PhD (Brucellosis Lab)
PVS Evaluation, 2012 PVS Gap Analysis, 2014
20052007
• Discussion and needed for brucellosis diagnosis and control
20082010
• FAO-APHCA/OIE Regional Workshops (1st, 2nd and 3rd WS)
20102013
• OIE Lab Twinning Programmeon Brucellosis (France-Thailand)
20132014
• 4th FAO-APHCA/OIE/DLD Regional Workshop
• Bovine PT (2013 and 2014)
2008Present
•NIAH-DLD brucellosis related activities and lesson learned, ISO/IEC 17025:2005 and ISO 9001:2008, research collaboration
•FETPV, One Health
•OIE-PVS
20142015
• Application as OIE Ref Lab for Brucellosis
UnderstandingCommunication,
Team and trust
Coordination & Collaboration
Control brucellosis in the region
Brucellosis laboratory network in Asia-Pacific region
Regional Network and need support
from international organisations
Disease Control
• Regional Veterinary Research and Development Centers (8 Labs)
• Bureau of Disease Control and Veterinary Services, DLD.
• Bureau of Epidemiology, Department of Disease Control, MOPH
• National Institute of Animal Health