© SPEX SamplePrep, Inc. 2011
SPEX SamplePrep Freezer/Mill®
© SPEX SamplePrep, Inc. 2011
Why Cryogenic Grinding?
• Many analytical samples are too flexible or sensitive to be ground at ambient temperatures
• Samples are subject to degrading • Does not damage DNA or RNA
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The Basics
• Samples are chilled at Liquid Nitrogen (LN) temperatures and ground using a Magnetically-Driven Impactor – No mechanical linkages or bearings
to fail • Sealed Grinding Vials ensure
sample integrity • Touch screen display w/ stored
procedures for rapid process replication and consistency
© SPEX SamplePrep, Inc. 2011
Who Can Benefit From a Freezer/Mill®?
• Forensic Labs – DNA identification • Medical Labs– genetic research, disease
prevention • Hospital Labs – For genetic screening • Agricultural Labs – For crop development • Agricultural Veterinary Labs – For livestock
breeding and health • Archealogy Labs – Ancient DNA extraction • Public Health and Environmental Monitoring
Labs • QC Testing of Manufactured Goods • Electronics Companies – Grinding Electronic
Components
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The Freezer/Mill® Family
Sample Size
0.6 – 5 g 0.6 – 100 g 0.6 – 200 g
LN System
Manual Manual, Auto-Fill Option
Auto-Fill Only
Price $ $$ $$$
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6970EFM – Features & Benefits
• Two Models Available – Single Grinding Chamber (6970M)
• 1 Grinding Chamber + 1 Pre-Cooling Chamber
– Dual Grinding Chambers (6970D)
• Totally enclosed auto-fill system with no Liquid Nitrogen exposure
• Increased through-put – Up to 8 small vials or 2 large vials
(D model only)
© SPEX SamplePrep, Inc. 2011
6770 – Features & Benefits
• Cost efficient • Runs one Micro-vial or small vial per run • Perfect for smaller samples (5 grams or
less) • Additional area for pre-cooling samples • Unit has small counter footprint
© SPEX SamplePrep, Inc. 2011
6870 – Features & Benefits
• Versatile large grinding chamber – Runs a variety of vials, ranging from micro
vials through the large, even up to 4 of the small vials
– Allows for wide range of sample sizes • Available with Auto-Fill,
or without • Additional area for
pre-cooling samples
© SPEX SamplePrep, Inc. 2011
Freezer/Mill Accessories
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Freezer/Mill Vials & Vial Sets
Micro Small Mid-Size Large 0.1 – 0.5 mL 0.5 – 4.0 mL Up to 25 mL Up to 50 mL 0.1 – 0.5 g 0.5 – 4.0 g Up to 40 g Up to 100 g
• Standard Set • Stainless Steel End Plugs & Impactor with Polycarbonate Center
Cylinder • Polycarbonate
• Avoids metal contamination • Stainless Steel
• Best for Polymer applications • Cr-Free
• For RoHS/WEEE appliactions
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Freezer/Mill Auto-Extractor
6814 Auto-Extractor™ • Works with Small and
Large size vials • Increases throughput • Reduces operator effort
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Freezer/Mill Accessory Packages
Includes:
• 6754 Small Extractor
• 6755 Vial Rack • 6806 Multi-Vial
Adapter
Includes:
• 6884 Mid-Size Adapter for 6804 Large Extractor
• 6886 Mid-Size Grinding Vial Adapter Includes:
• 6804 Large Extractor
• 6805 Vial Rack
© SPEX SamplePrep, Inc. 2011
Freezer/Mill Liquid Nitrogen Handling
• Cryogenic Gloves
• Portable Dewars & Pressurized Tanks – Required for use with Auto-Fill units
(6870 + Auto-Fill & 6970EFM)
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Samples, Application Notes & Resources
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Before & After…
Leather
Corn
Bone A Collection of our Before &
After Samples
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Application Notes
• Extensive library of Freezer/Mill® applications
Examples • SP005 – RNA Extraction from
Cartilage Tissue Using Cryogenic Grinding
• SP006 – Preparation of Rice Husks for the Analysis of Their Contents
• SP007 – Grinding Polymers For Qualitative and Quantitative Analysis
Also Available:
© SPEX SamplePrep, Inc. 2011
Product Demonstration Videos