SpeedVac SPD1010 ConcentratorJiarong (JR) ZhouInjectables
IntroductionUV-Vis SpectrophotometerVerification of SpeedVac SPD1010 ConcentratorO UV-Vis as Analytical
InstrumentNew EquipmentO Functionality?O Applications?O Limits?
SpeedVac SPD1010 Concentrator
CentrifugeO Prevents bumpingVacuum PumpO Creates a vacuumThermal HeatO ConvectionRadiant Cover (RC)O Heats by radiationRefrigerated Vapor TrapO -50°C
ParametersPreHeatO 45°CRC On/OffTemperatureO 45°C ~ 80°C (±5°C)O No HeatHeat/Run TimeVacuum PressureO 5.1 torr ~ 30.0 torr (±0.1 torr)Vacuum Ramp RateO 1 ~ 5
Purpose & ProceduresReplicating a Possible ScenarioAspirin in 50/50 H2O/MeOHAnalytical λO Calibration curvePrepared samples below LOQO 0.4µg/mL Aspirin in 50/50 H2O/MeOHMethod DevelopmentO Bumping/FoamingO Over DryingO Denaturing
5mg/mL
0.5mg/mL
0.05mg/mL
Calibration Curve
0.00 0.05 0.10 0.15 0.20 0.250.00
0.20
0.40
0.60
0.80
1.00
1.20
f(x) = 5.68948717948718 x − 0.0281391025641026R² = 0.999809070143822
Aspirin in 50/50 H2O/MeOH
Concentration (mg/mL)
Abso
rban
ce
0.4µg/mL
Partially Concentrated & Combined
Concentrated & Reconstituted with 1mL Solvent
Absorbance (AU) 0.0020 0.0393 0.0142
Concentration (µg/mL) <LOQ 11.846 7.435
ResultsLoss of Sample!O Bumping/FoamingO Uneven drying?1.5mL Samples
It Works!O Able to dry/concentrate samplesQualitative vs Quantitative
ReferencesRoger ParksAnalytical Chemistry 2.0DailyMed – Bayer Aspirin TabletGenevac – All About BumpingIntegrated SpeedVac® System SPD1010/SPD2010 Instruction ManualThermo Scientific Savant SPD1010 and SPD2010 SpeedVac® ConcentratorsValidation of Analytical Procedures: Text and Methodology
Questions?