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SURFsara HPC Cloud 19 nodes, 32 cores and 256 GB RAM each 400 TB storage in total Maximum 40 Gb access to storage

Installation Automation Automatic tool installation

● Cloudman script with opennebula support

● Galaxy codebase● PostgreSQL DB & Sun Grid Engine● Common NGS tools, e.g., BWA, bowtie, samtools, etc.

Automatic reference dataset installation● Cloudbiolinux script● Common genome builds, hg18, hg19, mm9, tair10, etc.● Tool specific genome indexes for bowtie, BWA, etc.

NBIC Galaxy @ SURFsara HPC CloudMattias de Hollander, David van Enckevort, Leon Mei, Marc van Driel, Rob Hooft

System Architecture Implemented using Cloudman script NFS shared file system

● /mnt/galaxyTools: galaxy, 3rd party tools, ● /mnt/galaxyData: galaxy user data, postgres DB● /mnt/galaxyIndices: genomes, liftover chains

Persistent Master Node4core, 32G

Tools, User data,Genomes, 50TB

Dynamic Worker Node

Cloudman auto-scaling

NFS mountsInternet

Backup

Acknowledgement Enis Afgan and the members of NBIC Galaxy administration team NIOO, SURFsara, CTMM-TraIT

Contact: [email protected] visit: http://galaxy.nbic.nl

Usage Used successfully in a number of NGS courses organized by NBIC Base image was used by several project specific Galaxy instances running at SURFsara HPC Cloud

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