Molecular Mechanisms of Membrane Traffic
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Series H: Cell Biology, Vol. 74
Molecular Mechanisms of Membrane Traffic
Edited by
D. James Morn§ Department of Medicinal Chemistry HANS Life Science Research Building Purdue University West Lafayette, IN, USA
Kathryn E. Howell C & S Biology University of Colorado School of Medicine Denver, CO, USA
John J. M. Bergeron Department of Anatomy and Cell Biology McGill University Montreal PO H3A 2B2, Canada
Springer-Verlag Berlin Heidelberg GmbH
/
of at
74.
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this of
Proceedings of the NATO Advanced Research Workshop on Cell-Free Analysis of the Functional Organization of the Cytoplasm: Molecular Mechanisms of Membrane Traffic, held at Airlie, Virginia, USA, May 9-13, 1992
ISBN 978-3-662-02930-5
Library of Congress Cataloging-in-Publication Data. Molecular mechanisms of membrane traffic / edited by D. James Morre, Kathryn E. Howell, John J. M. Bergeron. p. cm. -(NATOASI series. H, Cell biology; voI. 74) "Proceedings of the NATO Advanced Research Workshop on Cell-Free Analysis of the Functional Organization 01 the Cytoplasm: Molecular Mechanisms 01 Membrane Traffic, held at Airlie, Virginia, USA, on May9-13, 1992"-T.p. verso. Inciudes bibliographical relerences and indexes. ISBN 978-3-662-02930-5 ISBN 978-3-662-02928-2 (eBook) DOI 10.1007/978-3-662-02928-2
1. Cell membranes-Congresses. 2. Biological transport--Congresses.1. Morre, D. James, 1935-. II. Howell, Kathryn E., 1939- .111. Bergeron, John J. M., 1946- . IV. NATO Advanced Research Workshop on CellFree Analysis 01 the Functional Organization 01 the Cytoplasm: Molecular Mechanisms 01 Membrane Trallic (1992: Airlie, Va.) V. Series: NATO ASI series. Series H. Cell biology; voi. 74. QH601.M65 1993 574.87'5-dc20 93-1200
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© Springer-Verlag Berlin Heidelberg 1993 Originally published by Springer-Verlag Berlin Heidelberg New York in 1993 Softcover reprint of the hardcover 1 st ed ition 1993
Typesetting: Camera ready by authors 31/3145 - 5 43210 - Printed on acid-Iree paper
Preface The study of membrane traffic in reconstituted cell-free systems has
generated an unprecedented amount of new information on the biochemistry, molecular biology and genetics of membrane-based molecular events that underly normal and abnormal cellular function. Many of the individual steps have now been isolated and dissected in simple systems that permit detailed molecular analyses of transport mechanisms and their regulation. Reconstituted events of intercompartment transport include inter-membrane recognition, and controlled membrane fusion-fission reactions.
Among the many advances is the growi ng awareness of a remarkabl e evolutionary conservation of many of the components involved in the many steps of membrane traffic, this realization has accelerated greatly the pace of progress in the field.
This book provides a collection of participant contributions from the 1992 Summer Research Conference, "Mol ecul ar Mechani sms of Membrane Traffi c, " jointly sponsored with NATO by the American Society of Cell Biology. The conference was held May 9-13, at the Airlie Conference Center in the Virginia countryside, near Warrenton.
The conference was attended by 158 scientists. A unique feature was the high proportion of young scientists among the participants. Approximately 65% were students, postdoctoral fe 11 ows and young investigators. Each attendee contri buted to the conference with either a pl atform or poster presentation.
The major focus of the conference was on new data that begi ns to provide, for the first time, a detailed picture of the molecular events of vesicular membrane traffic. Both the small molecular mass GTP-binding and trimeric G proteins were implicated in practically all membrane traffic steps and provided a major theme of the conference.
We thank the plenary speakers who contributed so much to the success and continuity of the program, and Dottie Doyle and the staff of the American Society of Cell Biology National Office for exquisite program organization and local arrangements. Appreciation is extended as well to Prof. Placido Navas, Chairperson of the NATO Advisory Committee, and Sarah Craw for capable assistance in the organization of this volume.
Fi nanci a 1 support for student scho 1 arshi ps provided by the Nat i ona 1 Science Foundation, National Institutes of Health and the Keith Porter Endowment for Cell Biology is gratefully acknowledged. Additional support
was provided by the Upjohn Company, Abbott Laboratories, Bristol-Myers Squibb Company, Corning Incorporated, Estee Lauder, Inc., JEOL U.S.A., Inc., Merck Research Laboratories, Monsanto Company and Rainin Instrument Company, Inc.
D. J. Morre K. E. Howell J. J. M. Bergeron
CONTENTS
Preface
ENDOPLASMIC RETICULUM, STRUCTURE, FUNCTION AND TRANSPORT TO GOLGI COMPLEX
Cell-Free Analysis of ER to Golgi Apparatus Vesicular Traffic ....... 1-15 D J Morre and D M Morre
Identification of a Novel Post-ER, Pre-Golgi Compartment Where Unassembled Monomers of Oligomeric Proteins Accumulate .... 17-34
T C Hobman, I Woodward, M Komuro and M G Farquhar
G Protein Regulation of Vesicular Transport Through the Exocytic Pathway ................................................... 35-44
W E Balch, H Plutner, R Schwaninger, E J Tisdale, H W Davidson, J Bourne, S Pind and F Peter
Fusion Rapidly Follows Vesicle Transport to the Target Membrane in Protein Transport Through the Golgi Apparatus In Vitro. A Reevaluation of Transport Kinetics Based on the Finding That the Glycosylation Used to Mark Transport, and Not Transport Itself is Rate Limiting in the Assay ..................... .45-51
R R Hiebsch and B W Wattenberg
Immunocytochemical Analysis of the Transfer of Vesicular Stomatitis Virus G Glycoprotein from the Intermediate Compartment to the Golgi Complex ......................................... 53-54
S Bonatti, L V Lotti, M R Torrisi, M C Pascale
Synthesis of Glycosyl-Phosphatidylinositol Anchor is Initiated in the Endoplasmic Reticulum ..................................... 55-56
J Vidugiriene and A K Menon
Protein Trafficking Along the Exocytotic Pathway .................... 57-58 W Hong
A Short N-Terminal Sequence is Responsible for the Retention of Inv~iant Chain (Ii) in the Endoplasmic Reticulum ......... 59-60
M-P Schutze, M R Jackson and P A Peterson
Isolation and Characterization of the Principal ATPase of Transitional Elements of the Endoplasmic Reticulum of Rat Liver ........ 61-62
L Zhang and D J Morre
A GTP Hydrolase Activity Purified from Transitional Endoplasmic Reticulum of Rat Liver Binds Retinol ...................... 63-64
J Zhao and D M Morre
A 38 kDa Protein Resident to Cis Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against a Subunits of Trimeric G-Proteins .................................... 65-66
A 0 Brightman, M Paulik, J B Lawrence, T Reust, C C Geilen, K Spicher, W Reutter, D M Morre and D J Morre
VIII
Acyl Transfer Reactions Associated with Cis Golgi Apparatus ......... 67-68 J B Lawrence, T W Keenan and D J Morre
Hepatic Bile Formation: Intracellular Trafficking of Bile Salts ..... 69-70 J M Crawford, S Barnes, R C Stearns, D L Hastings, D C J Strahs and J J Godleski
Wheat Storage Proteins as a Model System to Study the Mechanism of Protein Sorting Within the Endoplasmic Reticulum .......... 71-72
G Galili and Y Altschuler
IMPORT INTO MITOCHONDRIA, PEROXISOMES, CHLOROPLASTS AND VACUOLES
Determinants of Outer Membrane Protein Sorting and Topology in Mi tochondri a .............................................. 73 -79
G C Shore, H M McBride, D G Millar and J -M Li
Molecular Chaperones HSP70 and HSP60 in Protein Folding and Membrane Translocation .................................... 81-90
J Martin and F -U Hartl
Cytosolic Reactions in Mitochondrial Protein Import ................. 91-94 D M Cyr and M G Douglas
Human Bifunctional Enzyme and Its Import Into Peroxisomes ........... 95-98 G L Chen and M C McGuinness
Transport of Microinjected Proteins into the Peroxisomes of Mammalian Cells ........................................... 99-100
P A Walton, S J Gould and S Subramani
Isolation and Characterization of a Functionally Active Protein Translocation Apparatus from Chloroplast Envelopes ........ 101-104
K Waegemann and J Soll
Protein Insertion into the Outer Mitochondrial Membrane ............. 105-106 D Millar and GC Shore
The Membrane-Bound 95 kDa Subunit of the Yeast Vacuolar Proton-Pumping ATPase is Required for Enzyme Assembly and Activity ....... 107-108
M F Manolson, D Proteau, R A Preston, M E Colosimo, B T Roberts, M A Hoyt and E W Jones
The Traffic of Molecules Across the Tonoplast of Plant Cells ........ 109-110 B P Marin
Fusicoccin Binding and Internalization by Soybean Protoplasts ....... 111-112 M A Villanueva, R. Stout and L R Griffing
TRAFFIC WITHIN AND FROM THE GOLGI COMPLEX
TGN38 and Small GTP Binding Proteins are Part of a Macromolecular .... 113-116 Complex in the Trans-Golgi Network
K E Howell
IX
p-Cop, a Coat Protein of Nonclathrin-Coated Vesicles of The Golgi Complex, is involved in Transport of Vesicular Stomatitis Virus Glycoprotein ........................................ 117-126
R Duden, B Storrie, R Pepperkok, J Scheel, B Joggerst-Thomalla, A Sawyer, H Horstmann, G Griffiths and T E Kreis
Defining the Retention Signal in a Model Golgi Membrane Protein ..... 127-133 C E Machamer, M G Grim, A Esquela, K Ryan and A M Swift
Organization of the Glycoprotein and Polysaccharide Synthetic Pathways in the Plant Golgi Apparatus .............................. 135-144
L A Staehelin
CLIP-170, a Cytoplasmic Linker Protein Mediating Interaction of Endosomes with Mi crotubul es ............................... 145-157
J Scheel, J E. Rickard, P Pierre, D Hennig, P I Karecla, R Pepperkok, G Joggerst-Thomalla, A Sawyer, R G Parton and T E Kreis
Maturat i on of Secretory Granul es .................................... 159-162 S A Tooze
Fatty Acylation in Membrane Trafficking and During Mitosis .......... 163-166 D I Mundy
Rates of Synthesis and Selective Loss Into the Bile of Four Rat Liver Proteins and the Polymeric IgA Receptor ................... 167-170
L J Scott and A L Hubbard
Effect of Brefeldin A Treatment on the Resident Golgi Protein, MG160.171-172 P A Johnston and N K Gonatas
Inhibition of Cholera Toxin by Brefeldin A .......................... 173-174 P A Orlandi, P Curran and P H Fishman
Selective Association with Golgi of ADP-Ribosylation Factors, 20-kDa Guanine Nucleotide-Binding Protein Activators of Cholera Toxin .................................................... 175-176
S -C Tsai, R Adamik, R Haun, J Moss and M Vaughan
Intracellular Transport and Post-Translational Modifications of a Secretory Heat Shock Protein of Saccharomyces Cerevisiae .. 177-178
M Simonen, M Wikstrom, B Walse and M Makarow
Transport to the Cell Surface of Chlamydomonas: Mastigonemes as a Marker for the Flagellar Membrane ......................... 179-180
M Bernstein and J L Rosenbaum
LIPID TRAFFICKING PROTEINS OF THE PLASMA MEMBRANE AND THEIR INTERACTION
Lipid Transport From the Golgi to the Plasma Membrane of Epithelial Cell s. .................................................... 181-185
G van Meer, I L van Genderen, W van 't Hof, K N J Burger and P van der Bijl
x
Lipid Traffic to the Plasma Membrane of Leek Cells. Sorting Based on Fatty Acyl Chain Length ................................... 187-195
P Moreau, B Sturbois, L Maneta-Peyret, D J Morre and C Cassagne
Hexadecy1phosphocho1ine as a Useful Tool for Investigating Phosphatidy1cho1ine Biosynthesis and Sorting .............. 197-198
C C Gei1en, Th Wieder and W Reutter
Biosynthesis of the Scrapie Prion Protein in Scrapie-Infected Cell s. .•••.........•....•....••..••....•........••....•... 199-200
A Tarabou10s, D R Borchelt, A Raeber, D Avrahami and S B Prusiner
Protein Phosphorylation Regulates the Cellular Trafficking and Processing of the Alzheimer Beta/A4 Amyloid Precursor Protein .................................................. 201-202
G L Caporaso, S E Gandy, J D Buxbaum, T Suzuki, C Nordstedt, K Iverfe1dt, T B Ramabhadran, A J Czernik, A C Nairn and P Greengard
Targeting of the Polymeric Immunoglobulin Receptor in Transfected PCI2Ce11s ................................................ 203-204
F Bonze1ius, G A Herman, M H Cardone, K E Mostov and R B Kelly
THE ENDOCYTIC PATHWAY AND SIGNALS
Signals for Receptor-Mediated Endocytosis ........................... 205-214 I S Trowbridge, J Co11awn, S White and ALai
Regulation of Early Endosome Fusion In Vitro .•.......••....•..•••... 215-228 o Steele-Mortimer, M J Clague, L Thomas, J-P Gorve1 and Jean Gruenberg
Adaptins and Their Role in C1athrin-Mediated Vesicle Sorting ........ 229-236 M S Robinson, C L Ball and M N J Seaman
Endosomes and Cell Signalling ....................................... 237-244 B I Posner and J J M Bergeron
The Tubu1 ar Early Endosome .......................................... 245-267 J Tooze and M Hollinshead
Direct Comparision of the Endocytic Routes of F1uorescent1y-Labe11ed Lipids and Transferrin .................................... 269-272
S Mayor and F R Maxfield
Linkage of Plasma Membrane Proteins with the Membrane Skeleton: Insights Into Functions in Polarized Epithelial Cells ..... 273-283
W J Nelson
Cell Specificity and Developmental Variation in the Targeting Pathways of FRT Cells ..................................... 285-288
C Zurzolo
XI
Phosphoglucomutase is a Cytoplasmic Glycoprotein Implicated in the Regul ated Secretory Pathway ............................... 289-292
D Auger, P Bounelis and R B Marchase
Protein Targeting and the Control of Cl- Secretion in Colonic Epithel i a 1 Cell s .......................................... 293-296
A P Morris, S A Cunningham, D J Benos and R A Frizzell
Apical Membrane Protein Sorting is Affected by Brefeldin A .......... 297-299 C B Brewer and M GRoth
Structural Analysis of the Clathrin Triskelion ...................... 301-305 I S Nathke and F M Brodsky
Polarity in Neurons and Epithelial Cells: Distribution of Endogenous and Exogenous Ion Pumps and Transporters .................. 307-308
G Pietrini
Biochemical Properties and Expression of Tau Proteins in the Endocytic Compartment During Liver Regeneration ..................... 309-310
M Verges, W H Evans and C Enrich
Identification of Hepatic Endocytic Proteins Potentially Involved in Membrane Traffic ....................................... 311-312
C Enrich, M Verges and W H Evans
Studies on Sequence Requirements for Basolateral Targeting of the Polymeric Immunoglobulin Receptor in MDCK Cells ........... 313-314
B Aroeti and K Mostov
Endocytosis by Trypanosoma Brucei: Proteases and Their Possible Role in the Degradation of Anti-VSG Antibodies ............ 315-316
J D Lonsdale-Eccles, D J Grab, D C W Russo and P Webster
Receptor Mediated Endocytosis of EGF in a Cell Free System .......... 317-318 T E Redelmeier, C J Lamaze and S L Schmid
Transferrin on the Basolateral Surface Regulates Apical 59 Fe Uptake and Transport Across Intestinal Epithelial Cells .......... 319-320
X Alvarez-Hernandez and J Glass
Intracellular Trafficking of Salmonella typhimurium Within HeLa Epithelial Cells .......................................... 321-322
F Garcia-del Portillo and B B Finlay
Functional Analysis of Dynamin, a GTPase Meditating Early Endocytos is. .............................................. 323 -324
H S Shpetner, C C Burgess and R B Vallee
Identification of a Regulatory Domain in Dynamin .................... 325-326 J S Herskovits, C C Burgess and R B Vallee
Potassium Depletion Stimulates Clathrin-Independent Endocytosis in Rat Foetal Fibroblasts .................................... 327-328
Ph Cupers, A Kiss, P Baudhuin and P J Courtoy
XII
Clathrin Interaction with NADH Dehydrogenases of Rat Liver Plasma Membrane ................................................. 329-330
P Navas, J M Villalba, J C Rodriguez-Aguilera, A Canalejo and M I Buren
Putative Adaptor Proteins of Clathrin Coated Vesicles from Developing Pea ...................................................... 331-332
J M Butler and L Beevers
Uncoating of Plant Clathrin Vesicles by Uncoating ATPase from Peas .. 333-334 T Kirsch and L Beevers
LYSOSOMES AND DEGRADATION
Selective Degradation of Cytosolic Proteins by Lysosomes ............ 335-338 J F Dice
Role of Calcium, Protein Phosphorylation and the Cytoskeleton in HepatocyticAutophagy ..................................... 339-349
P 0 Seglen, I Holen and P B Gordon
Signals for Transport From Endosomes to Lysosomes ................... 351-358 J P Luzio, T Kuwana and B M Mullock
A New Receptor for Lysosomal Proenzymes ............................. 359-362 A H Erickson, G F McIntyre, G D Godbold and R L Chapman
A Protein Kinase/Lipid Kinase Complex Required for Yeast Vacuolar Protei n Sort i ng ........................................... 363 -366
J H Stack, P K Herman and S D Emr
The Morphology But Not the Function of Endosomes and Lysosomes is Affected by Brefe 1 din A ................................... 367 -370
S A Wood and W J Brown
Purification of the N-Acetylglucosamine-1-Phosphodiester s-N-Acetylglucosamindase from Human Lymphoblasts .............. 371-372
K-W Zhao and A L Miller
Altered Regulation of Protein Degradation in Tranformed Human Bronchial Epithelial Cells ................................ 373-374
H-K Lee, RAM Myers and L Marzella
An Examination of the Structure and Functions of the Bovine Vacuolar ATPase Using Antisense 01 igonucleotides ................... 375-376
J E Strasser, Y-x Pan, R Morris and G E Dean
MOLECULES IDENTIFIED IMPORTANT IN MEMBRANE TRAFFIC
Regulation of Endocytosis by the Small GTP-ase Rab5 ................. 377-385 R G Parton, C Bucci, B Hoflack and M Zerial
XIII
The Nucleotide Cycle of Sec4 is Important for its Function in VesicularTransport ....................................... 387-390
P Novick, P Brennwald, M D Garrett, M Moya, D Roberts and R Bowser
Activators of Trimeric G-Proteins Stimulate and Inhibit Inter-compartmental Golgi Transport In Vitro .................... 391-394
P J Weidman
Evidence of a Role for Heterotrimeric GTP-Binding Proteins and ARF in Endosome Fusion .................................... 395-398
L Mayorga, M I Colombo, J M Lenhard and P Stahl
The Small GTPase Rab4 Controls an Early Endocytic Sorting Event ..... 399-402 P van der Sluijs, M Hull and I Mellman
Protein Modifications and Their Significance in rab5 Function ....... 403-404 J C Sanford and M Wessling-Resnick
Low Molecular Weight GTP-Binding Proteins in Rough Endoplasmic Reticulum Membranes From Rat Liver and Rat Hepatocellular Carc i nomas. ............................................... 405 -406
J Lanoix and J Paiement
Low Molecular Weight GTP-Binding Proteins Associated with the Membranes Involved in Post-Golgi Transport of Rhodopsin ............. 407-408
D Deretic and D S Papermaster
Author Index ........................................................ 409-411
Subject Index ....................................................... 413-415