MICROSCOPE

Human naked eyes cannot observe objects with size smaller than 0.1 mm

Microscope is a tool that is used to observed microscopic objects that cannot be seen with the naked eyes.

Microscope Parts

MirrorMirror

Objective lensObjective lens

Hole Hole

DiaphragmDiaphragm

Eyepiece

Body tube

Revolver

Croase Croase Focus

Stage Stage

Stage ClipsStage Clips

BaseBase

ArmArm

Fine Focus Fine Focus

Parts of Microscope and their function

Eyepiece : to magnificate image you look through.

Parts of Microscope and their function

Coarse focus : focuses the image under low power

Parts of Microscope and their function

Body tube : connect the eyepiece to the revolving nose piece

Parts of Microscope and their function

Fine focus : sharpens the image under high and low magnification

Parts of Microscope and their function

Revolver : holds and turns the objectives into viewing position

Parts of Microscope and their function

Low-power objective Lens : contains the lens with lower power magnification

Parts of Microscope and their function

High-power objective Lens : contains the lens with high (the most) magnification.

Parts of Microscope and their function

Arm : support the body tube.

Parts of Microscope and their function

Stage : platform used to support the microscope slide

Parts of Microscope and their function

Stage clips : hold the microscope slide in place

Parts of Microscope and their function

Diaphragm : regulates the amount of light entering the body tube

Parts of Microscope and their function

Mirror : allows light to go upward through the diaphragm, the specimen, and the lenses

Parts of Microscope and their function

Base : provides support for the microscope

How to figure out the Magnification?

Low Power 10 X 10 X 100 X

Eyepiece Objective Lens Magnification

High Power 10 X 45 X 450 X

Working with your microscope !

1. Place the microscope on flat surface. The arm should be toward you.

2. Look through the eyepiece. Open the diaphragm.

3. Adjust the mirror until you can see the most light coming through the tube.

Seven easy steps Seven easy steps for beginners !for beginners !

4. Place the slide so that the material to be observed is in the middle of the hole on the stage.

5. Turn the nosepiece to the low- or medium-power objective- never start to the high-power.

6. Turn the coarse focus until the objective goes as far as it can without touching the slide.

7. Finally, look through the eyepiece and sharpens the image by turning the fine focus.

Attentions !!Attentions !!

1.1. Always carry the microscope by holding the Always carry the microscope by holding the arm with one hand and supporting the base arm with one hand and supporting the base with the other hand.with the other hand.

2. Do not touch the lenses with your finger.2. Do not touch the lenses with your finger.

3. Always focus first with lower-power objective.3. Always focus first with lower-power objective.

4. Do not use coarse focus when the high power 4. Do not use coarse focus when the high power objective is in place.objective is in place.

Once you have examined the specimen under low power, try this:

fiddle with the mirror. Sometimes you can change the light and see the specimen in a different way.

as you look through the eyepiece, try moving the as you look through the eyepiece, try moving the slide to see different parts of the specimen. This slide to see different parts of the specimen. This takes practice. takes practice.

try a high-power objective, but expect the light to be try a high-power objective, but expect the light to be dimmer. You will probably have to change the focus dimmer. You will probably have to change the focus a bit. a bit.

Position of Object Glass and Cover Glass

Student Activity:

Observing Pond Water

Have you ever thought that life exists in a very small drop of water?

You will not be able to observe it with your naked eyes.

Therefore, you need to conduct an observation under a microscope

MicroscopeObject glassCover glassPipettePond WaterChemical glass

100 ml

What do you need?

What should you do?

Select a water environment (river, gutter, well, etc), in your school area and take samples of water. Observe what is inside the water by taking a drop of water

Observe and draw the organism seen Observe and draw the organism seen under the microscopeunder the microscope

How many kinds of organisms can you How many kinds of organisms can you observe?observe?

Observation Result