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BUCKMAN LABORATORIESBUCKMAN LABORATORIES
MICROBIAL ACTIVITIES SURVEY
IN
Kilang Gula FELDA Perlis,
Perlis
Malasia
Prepared by
BUCKMAN LABORATORIES (Asia) Pte Ltd
Chua Hi Ee
Mar!h "##$
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REPORT ON MICROBIAL ACTIVITIES SURVEY IN KGFP
INTRODUCTIONSu%ar is a& i'prta&t e&er%y a&d d
sur!e r 'i!rbia %r*th a&d reprdu!ti&+,eterirati& su%ar
!aused by 'i!rr%a&is's is a*ays a !&!er& i& the
su%ar'ii&% pr!ess+ Busa& -#./ has bee& i&trdu!ed at
K01P t 'i&i'i2e the de%radati& su%ar due t 'i!rr%a&is'
a!ti3ity+ I& rder t 'easure a&d e&sure the
ee!ti3e&ess the bi!ide4 Bu!5'a& te!h&i!a ser3i!e tea'
*ith assista&!e r' K01P has !arriedut 6 sets 'i!rbia
e&u'erati& tests &7site r' "# tht "-stMar!h "##$+
TEST OB!ECTIVES
The pri'ary purpse the sur3ey *as t deter'i&e the 'i!rbia
a!ti3ities i&K01P su%ar 'ii&% pr!ess ater dsi&% *ith
Busa& -#./+
The se!&dary purpse *as t e3auate the ee!ti3e&ess
sa&itati& i& the 'iby t*i!e usi&% the 'i
e8isti&% !ea&i&% a%e&t+
LABORATORY E"PERIMENT DESIGNSa'pi&% pi&ts *ere
ide&tiied ater dis!ussi& *ith Te!h&i!a ,ept+ i!er4
Tua& Ha9i
Abu Ba5ar 6 sets sa'pes *ere !e!ted & "#tha&d "-stMar!h
"##$+
Sa#$les ID Tes% De%ails
-st'i Pate !u&t
-+ tta aerbi! ba!teria
"+ Leu!&st! sp+
.+ a!id7prdu!ers
6+ yeasts
/+ 'uds
Te'perature
pH
"&d 'i
,iuser :ui!e
Mi8ed 9ui!e (Ta&5 C-)
.rd'i
6th'i
Press *ater
Press *ater ater !ariier
I'bibiti& *ater
Set I ; "#th--##Set II ; "#th-
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Cuti3ati& Mi!rr%a&is'sPate !u&t a&aysis *as
!&du!ted by seria diuti&s the sa'pes4 *ed byspread pate
'ethd+ The sa'pes *ere i&!ubated at 6/>C r .#7.? hurs+
Aapparatus *as sterii2ed prir t the test+
Sa&itati& Sur3ey
The Nutrie&t A%ar (NA) pates a&d Saburud ,e8trse A%ar
(S,A) *ere prepared i&rda! pates+ The NA *as used t
deter'i&e aerbi! ba!teria *hie the S,A *as usedt !uti3ate
yeasts a&d 'ds+ The pates *ere e8psed t the air a&d tu!hed
& thesura!e the 'a!hi&e t !apture the 'i!rr%a&is's+ The
test *as d&e t*i!e tdeter'i&e the ee!ti3e&ess 'is
sa&itati&+
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LABORATORY TEST RESULTS
Kilang Gula FELDA Perlis
Firs% R&un' Ta(ing Sa#$les )&r E*is%ing Bi&+i'e
-%. Mar+. --/
NO SAMPLES TEMP01C2 TIME $3 MICROORGANISMS COUNT
TAB LEUCONOSTOC s$4 AP YEAST 5 MOULD
- -st Mi ." -#"# 6+/. /+@?E#/ -#### N, N,
" "&d Mi ./ -#"- 6+// 6+
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Kilang Gula FELDA Perlis
Firs% R&un' Ta(ing Sa#$les )&r BUCKMAN Bi&+i'e
7s% Mar+. --/
NO SAMPLES TEMP01C2 TIME $3 MICROORGANISMS COUNT
TAB LEUCONOSTOC s$4 AP YEAST 5 MOULD
- -st Mi .. -#"# 6+
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The tta 3iabe !u&ts resuts *ere &t %d t a!hie3e the
purpse
the studies due t the u&e8pe!ted hi%h 'i!rr%a&is's
!u&t+
The pr uaity !rushed !a&es ead t hi%h 'i!rr%a&is's
!u&t i& 1irst
E8press :ui!e4 *ith abut -#
