Methodology and Project Planning
STR1 Kawashima
Methodology
Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples
Soil samples from various oil-contaminated areas in Guimaras will be obtained and
stored in 50 mL conical tubes at room temperature. To isolate possible hydrocarbon-
degrading bacteria from each sample, a pinch of soil will be inoculated into 50 mL of
liquid minimal medium (for 1 L: 0.5g KH2PO4, 2.0g KNO3, 1.0g MgSO4 7H2O, 0.0012g
CaCl2•2H2O, 0.002g FeSO4•7H2O) with 50 μL of bunker oil (Petron Corporation,
Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one
week. Once a visible change in turbidity is observed, an applicator stick will be
dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar
(Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and
incubated overnight at 37°C. Isolated bacterial colonies of different colonial
morphologies will be picked and subcultured into separate LB agar slants.
Isolation of Hydrocarbon-degrading Bacteria from Guimaras Soil Samples
Soil samples from various oil-contaminated areas in Guimaras will be obtained and
stored in 50 mL conical tubes at room temperature. To isolate possible hydrocarbon-
degrading bacteria from each sample, a pinch of soil will be inoculated into 50 mL of
liquid minimal medium (for 1 L: 0.5g KH2PO4, 2.0g KNO3, 1.0g MgSO4 7H2O, 0.0012g
CaCl2•2H2O, 0.002g FeSO4•7H2O) with 50 μL of bunker oil (Petron Corporation,
Philippines), and subjected to incubation while shaking at 37ºC at 225 rpm for one
week. Once a visible change in turbidity is observed, an applicator stick will be
dipped into the suspension culture and streak plated on Luria-Burtani (LB) agar
(Pronadisa, Madrid, Spain). One streak plate will be made for each soil sample and
incubated overnight at 37°C. Isolated bacterial colonies of different colonial
morphologies will be picked and subcultured into separate LB agar slants.
Project Management
Project Management Tools
Process Flowchart
GENERAL description of an activityEach activity is bounded in
a RECTANGLE
ARROWS indicate what’s next
Go to Trinoma
Get tickets
Buy snacks
Watch movie
Eat dinner
Go home
Gantt Chart
1 4 60
17:00 20:00 21:0016:00
Time, hours
18:00 19:00 22:00
2 3 5
current
Go to Trinoma
Get tickets
Buy snacks
Watch movie
Eat dinner
Go home1 4 60
17:00 20:00 21:0016:00
Time, hours
18:00 19:00 22:00
2 3 5
current
Names are in imperative form
Time increments run along the bottom, in hours/days/weeks/months
Task names take up one row
Go to Trinoma
Get tickets
Buy snacks
Watch movie
Eat dinner
Go home1 4 60
17:00 20:00 21:0016:00
Time, hours
18:00 19:00 22:00
2 3 5
current
Bars represent expected duration of the task
Arrows connect prerequisite tasks
Shaded bars mean finished tasks while unshaded bars are unfinished
Shading tells how complete a task is
(think percentage)
Go to Trinoma
Get tickets
Buy snacks
Watch movie
Eat dinner
Go home1 4 60
17:00 20:00 21:0016:00
Time, hours
18:00 19:00 22:00
2 3 5
current
This line marks the current time/date
Fully shaded bars left of the line are completed tasks
Unshaded bars right of the line are tasks
yet to be started
Unshaded bars LEFT of the line are
behind schedule
Network ChartA=1 B=2
C=1
D=2 E=1 F=1
Code Description Duration (hr)
A Go to Trinoma 1
B Get tickets 2
C Buy snacks 1
D Watch movie 2
E Eat dinner 1
F Go home 1