1. NUR FAUZANA BT ZULKIFLI (G 1027668)
2. NOR ‘AIDAH BT LUKMAN (G 1027312)
3. WAN SUHAILA BT WAN ISMAIL (G 1022282)
EDT 5303TECHNOLOGY AND
INNOVATION IN EDUCATION( GROUP 12)
TOPIC : MECHANISM OF ENZYME ACTION
CONTENTCONTENT
LEARNING OUTCOME1
2
4
5
6
INTRODUCTION
DEFINITION OF ENZYME
MECHANISM OF ENZYME ACTION
ASSESSMENT
3 PRIOR KNOWLEDGE
LEARNING OUTCOMELEARNING OUTCOME
4.10 : Explain the
mechanism of enzyme action
INTRODUCTIONINTRODUCTION
Do you realize the term of COENZYME
on detergent products?
CLICK HERE !!
PRIOR KNOWLEDGEPRIOR KNOWLEDGE
Maltose
RECALL !!!
Water+ Glucose + Glucose
ENZYME maltase BREAK DOWN maltose into 2 molecules of glucose
Prior knowledgePrior knowledge
Cloth
Stains
Enzymes
How the stains have been removed from the cloth?
DEFINITION OF ENZYMEDEFINITION OF ENZYME
Enzymes are biological catalyst that direct or guide almost all cellular reactions.
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
‘LOCK AND KEY’ HYPOTHESIS
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
Enzyme
Substrate
Enzyme-substrate complex
Product
Product
EXPLANATION EXPLANATION EXPLANATION
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
A specific substrate molecule arrives at the active site of the
enzyme molecule.
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
The substrate molecule binds to the active site to form an
enzyme-substrate complex which is very unstable.
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
Product molecules leave the active site of the enzyme.
The enzyme is free to take another substrate molecule
into its active site to catalyse another reaction.
MECHANISM OF ENZME ACTION
MECHANISM OF ENZME ACTION
Sucrase
Sucrose
Maltose
Sucrose can bind to active site of sucrase
Maltose CANNOT bind to active site of sucrase
Enzymes have
specific sites
called active site which bind to specific substrates
Which substrate can bind to active site of enzyme sucrase?
assessmentassessment
Question 1 Question 2
Question 3 Question 4
Question 1Question 1
Fill the blank with the correct answer.
1. A specific ______________ molecule arrives
at the active site of the ______________ molecule.
2. The substrate molecule binds to the active site of the
enzyme to form an ______________.
3. The enzyme is free to take another substrate molecule
into its ______________ to catalyse another reaction.
4. ______________ molecules leave the active site of the
enzyme.
substrate
active site
enzyme
Product
enzyme-substrat
e complex
Question 2Question 2
A. Enzyme reaction is specific
Which of the following shows the characteristic of enzymes?
B. Enzyme reaction is influenced by pHC. Enzyme reaction is influence by
temperatureD. The structure of the enzyme change at
the end of the reaction.
Question 3Question 3
Protease
Dissolve starch stains
on cloth
Amylase
Lipase
Ripening cheese
Dissolve protein stains
on cloth
Question 4Question 4
Enzyme is used to wash away the blood stain on a shirt.
Explain how the enzyme acts on the blood stain at a maximum rate.
(3 MARKS)
ANSWER :Blood is made up from proteins. Protease in detergent will break down protein into amino acids. This reaction
occur effectively at 40 ºC which is optimum temperature for enzyme action.
THANK YOU ….