INTRODUCTION The bacteria genera most frequently isolated are:

Lactic acid microbiota in

postpartum cow and her calf

Mariona Rossell Garcia

June 2017

OBJECTIVES Determination of lactic acid bacteria in cow’s colostrum and milk, and the faeces of

their calves Comparison of the isolated microbiota

3 dairy farmsOsona

115 colostrum and milk samples 31 faeces samples

MATERIALS AND METHODSAgarManRogosaSharpewithcysteine(MRS+C)

Differentgeneraoflacticacidbacteria

SpecificforBifidobacteriumspp.

Aerobicconditions

Anaerobicconditions

Microaerophileconditions

AgarManRogosaSharpe(MRS)

37ºC24-48h

Incubationconditions Identificationmethods

GramStainCatalaseandOxidaseTest

API50CHL

RESULTS

CONCLUSIONS ✴ The genera isolated are Bifidobacterium spp., Lactobacillus spp. and Lactococcus spp. No many differences between samples and

farms ✴ The counts of the genera Bifidobacterium are higher in colostrum while the counts of Lactobacillus and Lactococcus are higher in milk ✴ The presence of the same genera between meconium and faeces supports the hypothesis of a non sterile gastrointestinal tract of the

neonate ✴ Exists a marked variability between individuals and cow’s quarters. For a representative sample of the individual, should be sampled

each of the quarters

Isol

atio

n fre

quen

cy

0 %

7,4 %

14,8 %

22,2 %

29,6 %

37 %

Lactic acid bacteria genera

Bifidobacterium spp. Lactobacillus spp. Lactococcus spp.

Colostrum Milk Faeces

Graphic1.Lacticacidbacteriageneraisolatedincolostrum,milkandfacessamples

32,95%

35,29%

33,33%

34,09%

35,29%

36,67%

32,95%

29,41%

30,00%

ColostrumandMilk

Kocuria Staphylococcus Streptrococcus Lactic acid bacteria Bifidobacterium, Lactobacillus, Lactococcus and Leuconostoc

PROBIOTICS??

Lactobacillus

Bifidobacterium

Escherichia

Shigella

FaecesNEONATALCALFDIARRHEA

Bifidobacteriumspp–Lactobacillusspp.andLactococcusspp.

LactobacillusplantarumLactococcuslactis

Colostrum=MilkFaecesProbiotics’features

MeconiumPossiblematernalorigin

Bifidobacteriumspp.ismorefrequentlyisolatedinmilksamples,butitscountsarehigherincolostrum

Colostrumandmilksamples:Highvariabilitybetweencow’squartersandbetweenindividuals

Faecessamples:Highvariabilitybetweenindividuals

Nomanydifferencesbetweenfarms

Graphic2.Representativeboxplotofcows’quarterscountofeachindividual,sortedbyfarms

Farm1Farm2Farm3