KOTAGIRI MELODY, LIMGENCO MARTHA, MENDOZA MIGUEL, NEVADO PAOLO, SANDOVAL PATRICIA, SOLIVEN MONIQUE
Effects of Diesel Inhalation, Alcohol Consumption and Sugar
Intake on Learning and Coordination
Introduction1) To be able to determine
the correlation of diesel inhalation, alcohol consumption, and sugar intake with the time needed to learn the course of a maze
2) To be able to determine the effects of diesel inhalation, alcohol consumption, and sugar intake on coordination.
Methodology
a. Alcohol Consumption
b. Diesel Inhalation
c. Sugar Intake
5%
10%
gauze
8 mL diesel
chicken wire cage
glucose
50%
5%
improvised gavage
improvised gavage
Results
Table 1. Effect of Alcohol Intake on Learning and Coordination
* 0.2 mL volume of 5% and 10% alcohol were administered through an improvised tube
Mouse Observations Time to Finish Maze (mm:ss)
1 (5% alcohol)* Normal movement speed, but kept missing the right path
7:50
2 (10% alcohol)* Lacking coordination in movement, followed the same path over and over again
More than 15 minutes
Control Normal 2:20
Results
Table 2. Effect of Exposure to Diesel on Learning and Coordination
*amount of diesel 8 mL in Petri Plate covered in gauze
Mouse Observations Time to Finish Maze (mm:ss)
1 (15 mins.) Slow movements, didn’t eat food
03:01
2 (30 mins.) Slower movements, didn’t eat food
12:41
Control (no diesel inhalation)
Normal 02:10
Results
Table 3. Effect of Sugar Intake on Time to Finish the Maze
*A 15-minute cap was placed on the time, and Mouse 2 was not able to finish the maze.
Mouse Observations Time to Finish Maze (mm:ss)
1 (50% glucose) Faster movement, ate the food at the end of the maze
3:30
2 (5% glucose) Fast movement, hyperactive, climbed over the maze
15:00*
Control Normal 2:20
Discussion
a. Alcohol Consumption- Ethanol as a central nervous
system depressant- Nigrostriatal pathway- Memory, attention, sleep,
coordination, and judgment- Coordination: alcohol impairs
reaction time and mental acuity
- GABA and NMDA
Discussionb. Diesel Inhalation- Diesel vapour inhalation:
effect on the mobility - Hydrocarbons : decreases
the sensitivity of postsynaptic neurons ; hypoxia
- Respiratory depressionc. Sugar Intake- Increased blood sugar levels
in their blood- Influence memory, motor
function and sleep patterns
Conclusion
- Study verified hypothesis about alcohol effects - Sugar intake results induced hyperactivity but does not
give a direct relation to coordination and learning patterns
- assumption: hydrocarbons are responsible for the sluggish movements of the mouse.
- long-term effects may also develop from regular and high - concentration exposure of diesel fuel ﴾e.g. lipoid pneumonia).
- A detailed study is therefore recommended to determine the specific compound(s) in diesel fuel and the pathway impaired by this compound(s) which cause the signs of toxicity.