Project MULTIFOREVER is supported under the umbrella of ERA-
NET Cofund ForestValue by ANR (FR), FNR (DE), MINCyT (AR),
MINECO-AEI (ES), MMM (FI) and VINNOVA (SE). ForestValue has
received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement N° 773324.
Kick-Off Seminar, Helsinki, 23-24 May 2019
MULTIFOREVER (36 months, 2019-2022)
Towards intensification of conifer production through multi-varietal forestry based on somatic embryogenesis
Jean-François TRONTIN, FCBA technological institute, FranceAndrea RUPPS, Humboldt University of Berlin (HUB), Germany
How it mayhave begun ...
➔Wood biomass / intensively managed plantations
Innovative sustainable management of multifunctional forests
Topic A
The first contact …
BUT
Forestry: mainly conifersTotal EU: 113 Mha
Coniferous sp.
Non coniferous sp.
Wood demand increasing(EU-28, roundwood/1000 m3, Eurostat)
Mainly planted forests➔ Improved varieties
Mostly through seed orchards
Seed-basedForestry➔ Sexual
reproduction
Seed shortage
Seed yieldPine, kg seeds/hl cones
(Boivin & Davi 2016)
Climate change ➔ Stress(Lindner et al. 2014)
Lesspollen
Lessflowers
Flower abortion
Morepests
Seedfeeding
Coneabortion
Cuttings
IDEA!➔ Vegetative,
clonal propagation
1TreeVariety
Limited successin conifers
(recalcitrance)BUT
Our great idea!Forestry: mainly conifersTotal EU: 113 Mha
Coniferous sp.
Non coniferous sp.
Wood demand increasing(EU-28, roundwood/1000 m3, Eurostat)
Mainly planted forests➔ Improved varieties
BUTMostly through seed orchards
Seed-basedForestry➔ Sexual
reproduction
Seed shortage
Seed yieldPine, kg seeds/hl cones
(Boivin & Davi 2016)
Climate change ➔ Stress(Lindner et al. 2014)
Lesspollen
Lessflowers
Flower abortion
Morepests
Seedfeeding
Coneabortion
Cuttings
IDEA!➔ Vegetative,
clonal propagation
TreeVariety1
Limited successin conifers
(recalcitrance)BUT
EmbryosSomatic
NEW!Mix of clonal, tested varieties
Multi-varietalForestry➔ Asexual
reproduction
LESSPROBLEM!
Multi-varietal planted forests➔ HIGHLY productive varieties
SUCCESS!Somatic
seedlings!
➔ By somaticembryogenesis!
SeedEmbryo1
Conifer MVF:a new strategy for a greener, forest-based bioeconomy!
Forestry: mainly conifersTotal EU: 113 Mha
Coniferous sp.
Non coniferous sp.
Wood demand increasing(EU-28, roundwood/1000 m3, Eurostat)
Seed shortage
Seed yieldPine, kg seeds/hl cones
(Boivin & Davi 2016)
Climate change ➔ Stress(Lindner et al. 2014)
Lesspollen
Lessflowers
Flower abortion
Morepests
Seedfeeding
Coneabortion
IDEA!
EmbryosSomatic
NEW!Mix of clonal, tested varieties
Multi-varietalForestry➔ Asexual
reproduction
LESSPROBLEM!
Multi-varietal planted forests➔ HIGHLY productive varieties
SUCCESS!Somatic
seedlings!
➔ By somaticembryogenesis!
SeedEmbryo1
7 expert teams in conifer somatic embryogenesis
Finland
Germany
Sweden
Spain
France
Argentina
8 important conifer species in EU (7) and Argentina (2)
European larchHybrid larchDouglas-fir
Hybrid larch
Loblolly pineHybrid pine
Maritime pineLoblolly pineDouglas-fir
Radiata pine
Norway spruce
Our 4 scientific & technical challenges
WP1SE / trees
(initiation)
WP2SE / seeds
(optimisation)
WP3MVF trials
(setup)
WP4Scaling-up!
(➔market)
Basic & appliedresearch (TRL 1-3)
Advanced research(TRL 2-4)
Technologicaldemonstration (TRL 5)
Advanced research& demonstration (TRL 3-5)
« The Holy Grail »« Streamline the process! »
« It’s the mix that matters! »« Who will make the big bucks? »
InitiationMultiplication
CryopreservationMaturation
GerminationAcclimatization
Field trialsMVF demo
Scale-upAutomationCost-efficiency
WP1SE / trees
(initiation)
WP2SE / seeds
(optimisation)
WP3MVF trials
(setup)
WP4Scaling-up!
(➔market)
Basic & appliedresearch (TRL 1-3)
Advanced research(TRL 2-4)
Technologicaldemonstration (TRL 5)
Advanced research& demonstration (TRL 3-5)
« The Holy Grail »« Streamline the process! »
« It’s the mix that matters! »« Who will make the big bucks? »
Our communication & coordination plans
WP5Dissemination & Communication activities➔ Scientists, stakeholders, general public « You just don’t know it yet »
Online / documentary com.On-site com. / technical toursDissemination / exploitation
WP6Project (double) coordination
« Together we are stronger »
Internal networkingConsortium agreementForestValue follow-up
National follow-up
Our transnational support
Stakeholders (8 organizations from 5 countries)
Funding agencies
Jean-François TRONTINCoordinator (France)
Andrea RUPPSDeputy Coordinator (Germany)[email protected]
Contact details
Isabelle [email protected]
Diego A. [email protected]
PROJECT OBSERVERS:
Thank you for your attention!
The increasingly important role that conifer multi-varietal forestrycould play in the EU and Argentina to deploy high-yield varieties
adapted to environmental and socio-economic constraints
Cost efficiencyScaling-upMarket analysisCommercialization concept
Multi-varietal forestryVegetative propagationSomatic embryogenesis
Demonstration trialP1 - Trontin JF, Gallou A (FCBA)P2 - Rupps A, Raschke J (HUB)P3 - Lelu-Walter MA, Teyssier C, Poitelon C (INRA)P4 - Gauchat ME, Vera Bravo C, Boleso MA (INTA)P5 - Aronen T, Varis S, Tikkinen M (LUKE)P6 - Moncaleán P, Montalbán I, Ziluaga Amigó I (NEIKER)P7 - Egertsdotter U, Dobrowolska I, Street N, Eman A (UPSC)