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January 2006, Volume - 1
January 2006, Volume - 1
Genetix Biotech Product Update
REPLI-g KitFor High fidelity whole genome application from small or
precious samples, based on Multiple Displacement
Amplification (MDA) technology,
Benefitsl Reproducible whole genome amplification from a
variety of starting materials — including genomic DNA,
fresh or dried blood, buffy coat, and morel Highly uniform amplification — across the whole
genome with minimal amplification biasl Only one sample collection required — obtain unlimited
DNA for all your downstream applicationsl Uniform DNA yield — enabling direct use in downstream
applications without quantification
Now Whole Genome Amplification (WGA) is not a Difficult Task!
Eliminating Sample Limitations
Applications: REPLI-g amplified genomic can be used in a
variety of downstream applications, including:l SNP and STR genotyping analysis RFLP analysisl Comparative genome hybridization
WGA - Single Tube
Protocol
AlkalineDenature/Lysis
Neutralize
Repli-g Mix
Incubate at 30°C
>40 µg DNA
Post-itQiagen .................................... 1-4
Biometra ..................................... 1
MBI,Qiagen ................................ 4
Microflex, Amresco ...................... 4
Ambion, MBI ............................... 5
Spectrum, Cayman ...................... 6
Ambion, Amresco ........................ 6
Tosoh-Bioscience, Virogen ............ 6
Kimble Pharma ............................ 6
Vivascience ................................. 7
AB-Biodisk .................................. 8
Nunc .......................................... 9
Nunc, Nalgene, Peprotech ......... 10
Nalgene ............................... 11-12
mRNA Purification and cDNASynthesis in the same wellTurboCapture mRNA Kit – For rapid and easy mRNA purification
from cultured cells
l Speed — few protocol steps and minimal hands-on timel Simplicity — mRNA purification and downstream cDNA synthesis and PCR in
the same platel Versatility — mRNA purification also available in 8- and 96-well formatsl Automatable — kit is compatible with robotic workstations
TurboCapture mRNA Kits provide a fast and simple procedure for purifying mRNA.
Large numbers of samples can be processed simultaneously, making the kits
ideal for high-throughput applications such as compound screening and target
validation (e.g., by siRNA screening).
TurboCapture mRNA Kits are available in three formats:l TurboCapture 8 mRNA Kit — for mRNA purification in 8-well stripsl TurboCapture 96 mRNA Kit — for mRNA purification in 96-well platesl TurboCapture 384 mRNA Kit — for mRNA purification in 384-well plates
Fast and Easy Solutions for
Protein synthesis &
FractionationEasyXpressTM Protein Synthesis Kits – Fast and Efficient invitro synthesis of recombinant proteins
l Fast procedure - go from gene to protein in a single dayl High yields - up to 600 ug protein per ml reaction volume in just one hourl A scalable, convenient procedure - pre-aliquoted extracts, color-coded
reagents, and easy-to-follow protocolsl Significant time and cost-savings
- no cloning, transformation,
fermentation, or specialized
equipment required
The EasyXpress product range
has now been expanded with the
launch of several new kits:l EasyXpress biotin labeling kit-
guaranteed efficient detection or
site directed biotinylation for
interaction studiesl EasyXpress large-scale protein
synthesis kits-upto 5 mg purified
protein in just 4 hours.l EasyXpress protein Synthesis
Insect Kit-fully active and
correctly folded mammalian
proteins with posttranslational
modfifications.
& Improved
l Motorized heated lidl Unique: Motorized plate lifterl Small footprint, external controllerl Designed specifically for robotic systemsl Integrated cycler with external controllerl Proven Peltier technologyl Lid opening angle 1100
l Adjustable lid pressurel Small footprintl Available with 96 well (silver) or 384 well
(aluminum) blockl Low power consumptionl Communication with PC via serial RS232
protocoll Instrument provides extensive status report
T-Robot Thermocycler –Specially designed for integration in roboticenvironments
l With T-GRADIENT 12 different annealing
temperatures can be tested in single run.
l Temperature gradient of up to 40ºC
l Silver block for fast heating and cooling
l Easy to use software featuring gradient
l Software Touchdown. Time increment. Pause. Alarm. Online help. Temp.
Increment. Gradient calculator.
l Program memory Total capacity: 1500 steps 10 directories with 100 pgms
each, maximum 99 steps per program, maximum 99 cycles per loop
lBlock 96 well: silver, 48 well: silver, 384 well: Aluminum, in situ: Aluminum
lCapacity TGradient 96: 96 x 0.2ml tubes; 96 well Microplates; Strips
TGradient 48: 48 tubes 0.5ml
T-Gradient ThermocyclerFind the perfect annealing temperature in a single run
Test your new primer pairs with unknown
annealing temperature quickly and
optimize new protocols in short time
Three independent Thermocyclers in one
housing
Three different block formates available: 20
well 48 & combi welll T3000 based on latest Peltier Technology to
achieve excellent heating and cooling rates.l Better programming due to completely
revised software.l Bigger memory with program storage in
individual subdirectories.l Runs three programs at same time
Three heated lids with
automatic pressure
control (smart Lid
Technology)
Context sensitive soft
keys.
Program stored in
individual subdirectories
Small, Strong and hot!
New
Please askfor new
catalogs
NEW
NEW
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A general-use kit for reproducible fractionation and enrichment., Separate proteins according to their cellular location., Efficiently isolate and subfractionate active nucleic acid binding proteins., Fast, efficient albumin and igG depletion of serum and plasma samples., Separation of phosphorylated and unphosphorylated proteins., Highly Specific isolation of Glycoproteins
Qproteome kits - making the proteome more manageableA major problem facing protein researchers is the sheer complexity of the proteome. Purification and analysisof low-abundance species is especially problematic. QIAGEN has used its expertise in sample preparation todevelop the Qproteome range of kits, for standardized and reproducible protein fraction and depletion.
Qproteome Kit Offers:• Reduced sample complexity according to highly specific separation criteria — efficient
isolation of targeted subsets of proteins for easier analysis of low-abundance species• Simple, easy-to-use kit formats — no specialized equipment required• Highly reproducible, standardized separation, reliable results, time after time• Intact, native-conformation proteins — suitable for enzymatic assays and other activity-
based downstream applications
Cat No. Qproteome kit Protein categories Sample size Fractions isolated Price/Rs.
37900 Qproteome Bacterial Protein Prep Kit Soluble bacterial proteins 25 ml bacterial culture/ml lysis buffer Soluble bacterial proteins37901 Qproteome Mammalian Protein Prep Kit Total mammalian proteins 5–10 x 106 cells per ml lysis buffer Total mammalian proteins37512 Qproteome Soluble Protein Separation Kit Whole proteome 106–107 cells or 500 µl protein sample 0.5–4 mg protein in 6 fractions 16,834/-
(total protein 0.5–4 mg) separated according to protein solubility37502 Qproteome Cell Compartment Kit Organelle-/cell compartment-specific 5 x 106 cells 0.5–1 mg protein divided between 16,834/-
cytosolic, membrane, nuclear, & cytoskeletal protein fractions
37582 Qproteome Nuclear Subfractionation Kit Nuclear and nucleic-acid binding proteins 5 x 106 – 1 x 107 cells 1.5-2.5 mg total protein divided 20,287/-between cytosolic proteins,“insoluble” nuclear proteins (e.g.,histones), and 3 nucleic-acid–binding protein subfractions (50-100 µg each)
37541 Qproteome Total Glycoprotein Kit Glycosylated proteins 107 cells or 50 µl serum Separate enriched fractions 25,466/-37551 Qproteome Mannose containing 20-150 µg 25,466/-
Glycoprotein Kit glycoproteins carrying lectin-37561 Qproteome Sialic Glycoprotein Kit specific glycan moieties 25,466/-37571 Qproteome O-Glycan 25,466/-
Glycoprotein Kit
37521 Qproteome Albumin/IgG Depletion Kit Serum and plasma proteins 25 µl serum or plasma 0.5–0.8 mg albumin and IgG-free 18,560/-serum or plasma protein sample
37101 Phosphoprotein Purification Kit Phosphorylated proteins 1 x 107 cells Unphosphorylated proteins and 22,733/-phosphorylated proteins (typically10% total protein)
Efficient Fractionation of a Cell Lysate
Fractionation of an NIH-3T3 cell lysate sample
using the Soluble Protein Separation Kit. CL:
cleared lysate;M: markers.
kDa M CL 1 2 3 4 5 6
200 –
97 –
66 –
55 –
36 –
31 –
21 –
14 –
6 –
Fraction Number
Cell pellet (approx.5 x 106 cells)
Add ExtractionBuffer CE1
Add ExtractionBuffer CE2
Add Benzonase &Extraction Buffer CE3
Add ExtractionBuffer CE4
Fraction 4Cytoskeletal proteins
Fraction 1CytosolicProteins
Fraction 2CytosolicProteins
Fraction 3CytosolicProteins
Cell Compartment KitFractionation Procedure
Catalog No. Name Format Size282-2 Anaplasma Antibody Test Kit, cELISA cELISA, stripwell 2 plates282-5 Anaplasma Antibody Test Kit, cELISA cELISA, stripwell 5 plates273-2 Babesia caballi Antibody Test Kit, cELISA cELISA, stripwell 2 plates274-2 Babesia equi Antibody Test Kit, cELISA cELISA, stripwell 2 plates288-100 Bluetongue Virus Antibody Test Kit AGID 100 tests287-2 Bluetongue Virus Antibody Test Kit, cELISA cELISA, stripwell 2 plates287-5 Bluetongue Virus Antibody Test Kit, cELISA cELISA 5 plates284 Bovine Leukemia Virus Antibody Test Kit ELISA, stripwell 1 plate284-5 Bovine Leukemia Virus Antibody Test Kit ELISA, stripwell 5 plates
298 Bovine Spongiform Encephalopathy Antigen Test Kit, Immunohistochemistry Immunohistochemistry 50 tests275-5 Brucella Antibody Test Kit, ELISA ELISA, stripwell 5 plates289-2 Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA cELISA, stripwell 2 plates289-5 Caprine Arthritis-Encephalitis Virus Antibody Test Kit, cELISA cELISA, stripwell 5 plates400-200 Equine Infectious Anemia Virus Antibody Test Kit AGID 200 tests299-10 Escherichia coli Antigen Test Kit (K99 Pilitest) Latex agglutination 10 tests299-25 Escherichia coli Antigen Test Kit (K99 Pilitest) Latex agglutination 25 tests294 Malignant Catarrhal Fever Virus Antibody Test Kit cELISA, stripwell 1 plate280-2 Neospora caninum Antibody Test Kit, cELISA cELISA, stripwell 2 plates280-5 Neospora caninum Antibody Test Kit, cELISA cELISA, stripwell 5 platesTSE SET Prion Protein Detection Kit, IHC Immunohistochemistry 50 te
Veterinary Diagnostic
Test Kits & Reagents
FastLane Cell cDNA Kit It allows highly sensitive and reproducible results in real-time, two-step RT-
PCR as well as accurate detection over a wide linear range from 1 x 105 cells
down to one cell.l No RNA purification - from cells to cDNA in only 4 steps and less than
45 minutesl Short and simple workflow - easy parallel processing of several samplesl High sensitivity - detection of even low-abundance transcriptsl Detection of RNA only - accurate gene expression analysis with less
effort
l Reproducible Analysis of a Single cDNA Sample
No RNA purification For high-speed preparation of cDNA for use in real-time RT-PCR.
cDNA prepared from
HeLa ACC cells using
the FastLane Cell cDNA
Kit was analyzed 16-
times on the ABI PRISM
7700 using the
QuantiTect Probe PCR
Kit and the QuantiTect
Gene
Cancer genes were analyzed by real-time, two-step RT-PCR with SYBR Green detection
using QuantiTect Primer Assays. The cell lysis and RT steps were performed using either the
FastLane Cell cDNA Kit or a similar kit from Supplier AIV.
FastLane Cell cDNA Procedure
Applications
l Validation of siRNA-mediated gene knockdown
l Selection of potential drugs
l Detection of stimulated gene regulation
l Other gene expression analyses
NEW
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January 2006, Volume - 1
Suitable for collecting and
dispensing distilled water,
reagents, acids.
Integrated Solutions – Automated Sample Preparation
Models of efficiency – the affordable BioSprint 15 and BioSprint 96BioSprint workstations and kits give you:l A convenient automated procedure-saving
time and effortl A cost-efficient solution-economically priced
workstations for up to 15 or 96 samples per
runl Fast startup and immediate results-with easy
to use, preinstalled BioSprint protocolsl Flexibility and versatility-use the protocols
supplied or easily design your ownl Rapid purification of high quality genomic DNA-
ready to use in sensitive downstream
applications
Efficient Processing of Magnetic Particles
The BioSprint SystemBiosprint workstation and kits provide all you need
for rapid and cost-efficient automated sample
preparation-whatever your throughput needs. The
BioSprint System uses proven MagAttract
magnetic particle technology for purification of
high quality genome DNA for genotyping
applications.
Flurometric 384 well
HDAC Assay kit
Versatilel Analyze all HDACs from
yeast to humanQuantitative: MeasureHDAC activity froml Cell lysatel Purified proteinsl IP proteinsRapid: Process multiplesamplesl Simultaneouslyl Quicklyl ConvenientlyKit Componentsl 384 well black platel HDAC assay bufferl Activator solutionl HDAC assay standardl Trichostatin Al HeLa Nuclear extract
New
Well 1 Well 2
List Price
Rs. 2553/-
Carboys with Spigot,
low-density polyethylene:polypropylene spigot andscrew closure
Genetix Offers
The BioRobot EZ1 workstation purifies high-quality
nucleic acids from clinically relevant samples, such
as whole blood. 1-6 samples are processed in 15-20
minutes. Nucleic acids in sample lysates are isolated
in one step through their binding to the silica surface
of magnetic particles.
Reagents are supplied in pre-filled EZ1 Reagent
Cartridges, which ensures speed and convenience in
loading the BioRobot EZ1. Protocols for nucleic acid
purification are supplied on pre-programmed EZ1
Cards. These protocols provide both on-screen
instructions for the operator and operating commands
for the workstation.
BioRobot EZ1 Workstation delivers:l Rapid purification of high-quality nucleic acids from
a wide range of sample typesl Easy to use workstation requires minimal user
interactionl Affordable, slimline workstationl Flexibility and versatility to suit different types of
lab
BioRobot EZ1Workstation
l Safer, faster operation-process 6 samples in 15-20
minutes using pre-filled Reagentl Simple, walkway automation- just load the workable
and let it go!l Cartridges and pre-programmed EZ1 cards
Programming the workstation is as easy as using a
credit card
1. Insert the card containing the desired protocol into
the IC card slot and start the workstation
2. Add your samples (1-6) and prefilled and sealed
reagent Cartridge to the workstation. Samples are
processed automatically.
3. Remove from the workstation highly pure NA at a
concentration suited to your downstream application
in 1.5 ml microtubes.
A Inserting an EZ1 Card into the BioRobot EZ1. B Loading
sample tubes, elution tubes, filter-tips, and EZ1 Reagent
Cartridges.
EZ1 Kits related to Biorobot EZ1 Workstation
l EZ1 DNA Blood Kits
l EZ1 DNA Tissue Kit
l EZ1 RNA Cell Mini Kit
l EZ1 RNA Tissue Mini Kits
l EZ1 Virus Mini Kit
3steps
For
Rs. 1277/-
Nunc is with you all the way...Great Choice - Great Flexibility
Choice of Surface matters
PolySorp, with high affinity to molecules of a hydrophobic natureMediSorp, has a surface chemistry intermediate between PolySorp and MediSorp. Provides low background signalwith sample containing serum.MaxiSorp with high affinity to molecules with mixed hydrophilic/hydrophobic domains.
MultiSorp, with high affinity to hydrophilic molecules
l Choice of F,C and U bottomsl Raised rims on the wellsl Certified binding homogenicityl Alphanumeric orientation systeml When centrifuging Microwell plates use spacer plate as support.l Fit standard equipment
Plates intended for quantitative and qualitative solidphase immunoassay (eg. ELISA) and binding assay
Walkaway purification of
Nucleic acids has never
been so easy!
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:49 PM3
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January 2006, Volume - 1
Remember Santa CruzRemember Santa CruzRemember Santa CruzRemember Santa CruzRemember Santa Cruz When You DemandQuality AntibodiesQuality AntibodiesQuality AntibodiesQuality AntibodiesQuality Antibodies
l Phospho-Specific antibodiesl Intracellular FCM antibodiesl Multicolor FCM systeml Viral Proteinl Bacterial Proteinl Staining Systeml Isotype –Specific secondary antibodiesl F(ab’)2 Secondary antibodiesl Mounting Medial Whole Cell lysatel Acetylation Specific antibodies
And many more...Characterized for WB, IP, FCM & ELISA
RESTRICTION ENDONUCLEASES, NICKING & HOMING ENZYMES
New REases Catalog# Units Prototype Specificity 5'=>3'
LguI ER1931 100
ER1932 500
CseI ER1901 100
ER1902 500
EcoRII ER1921 200
ER1922 1000
HpyF3I ER1881 500
ER1882 2500
PscI ER1871 200
ER1872 1000
SgsI ER1891 300
ER1892 1500
TstI ER1911 100 TstI ^(8/13)CAC(N)6TCC(12/7)^
SapI GCTCTTC(1/4)^
HgaI GACGC(5/10)^
EcoRII ^CC(A/T)GG
DdeI C^TNAG
BspLU11I A^CATGT
AscI GG^CGCGCC
SUPRENO
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NEOPRO
Unlike conventional nitrile gloves, whichtend to fit poorly and constrict, causinghands to work harder and fatigue faster,FreeFormTMSE works with you,continuously conforming to the contoursof your hands as you move.Prodduct Code:Sizes & Quantity:FFS-INTXS - XL; 100/BOX l 1000/CASE
Supreno is a comfortable nitrileexamination glove formulated forsuperior strength and durability.Textured fingers offer better gripping inwet conditions. Interior polymer coatingmakes Supreno easy to don. Onlinechlorination processing means moreconsistent quality in every box, everytime.Prodduct Code:025Sizes & Quantity:SU-INTXS - XL; 100/BOX l 1000/CASE
Made from a soft polychloropreneformulation, NeoPro offers the barrierprotection of a synthetic with a fit andfeel like natural rubber latex. Morepuncture resistant than latex. Resists abroad range of chemical and biologicalhazards.Prodduct Code:Sizes & Quantity:NP-INTXS - XL; 100/BOX l 1000/CASE
Diamond Grip PlusTM is made from soft,premium natural rubber latex for acomfortable fit. Fully textured forenhanced sensitivity and control.Manufactured with integrated polymertechnologies for powder-free donningand product consistency.Prodduct Code:Sizes & Quantity:DGP-INTXS - XL; 100/BOX l 1000/CASE
Micro One is a highly versatileexamination glove that performs wellin demanding conditions. Made fromsoft latex, Micro One has a natural fitfor reduced hand fatigue. Lightlypowdered to go on easily. Smoothsurface allows for uncompromisedsensitivity.Prodduct Code:Sizes & Quantity:MO-INTXS - XL; 100/BOX l 1000/CASE
CE4 12” Hand Specific Gloves areWashed in microprocessor controlled,two-in-one, washer/dryer launderingsystem to provide very low surfaceparticulates, ionic extractables and non-volatile residues. These gloves areMulti-Class cleanroom compatibility.
DIAMOND GRIP PLUSTM
MICRO ONE
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Prodduct Code: SizesHSCE4-879-60 6.0HSCE4-879-65 6.5HSCE4-879-70 7.0HSCE4-879-75 7.5HSCE4-879-80 8.0HSCE4-879-85 8.5HSCE4-879-90 9.0
QUANTITIES: 1 pair per poly-wallet, 1 poly-walletper vacuum-sealed inner polybags; 10 inner polybagsper vacuum-sealed master polybags; 20 masterpolybags per poly lined carton of 200 pairs.
ISO 13485 certifiedEN 374 certifiedEN 455 certified
AMRESCO’s HTS NEXT GEL™ Kit
Save Time - Analyze up to
200 protein samples in 2 hours
Combination of a unique blend of
agarose & NEXT GEL™ Running
Buffer and can be used to analyze
SDS-denatured proteins using
standard horizontal gel apparatus
l Easy To Use - As simple as a
standard agarose, horizontal gell Non-Toxic & Biodegradable – A
unique blend of agarose
combined with the NEXT GEL™
Running Buffer, the HTS NEXT
GEL™ Kit contains no acrylamidel High throughput analysis - Up to
200 samples per gell Contains all reagents required to
run up to 5 gels (up to 1000
samples)l Cost-effective & Easy to use
protein analysisl Separates large proteins (0.2-10
million Daltons) depending on
the concentration of agarose
used (2% agarose yeilds 50 kD -
10 million kD; 4% agarose yeilds
14 kD - 212 kD)
Applications:
Protein expression library screening
Drug screening
Quality control stability studies
Cell and tissue extracts and lysates
Cell supernatants (activated vs. non-
activated)
Fractions from proteins
chromatography by HPLC or FPLC
MAIN PHOTO - HTS NEXT
GELTM (4%)- Stained withCoomassie R-250 Lane 1: MWStandards (wide) (Code: K494)(212 kDa-14.4 kDa). Remaininglanes represent random samplesof the following proteins: BSA (65kDa); Lactate Dehydrogenase (35kDa); Lysozyme (14.4 kDa); LiverHomogenate (14-100 kDa).
INSERT PHOTO - HTS NEXT
GELTM (4%)-Stained withCoomassie R-250 Proteinsamples used: MW Standard(Wide) (Code: K494) (212 kDa-14.4 kDa)’ MW Standards (M/L)(Code: J450) (97 kDa-14.4 kDa)’BSA (65kDa)’ CarbonicAnhydrase (35 kDa).
ZiP™ Reversible Protein Detection KitDestain in less than 5 minutes – Results in 15 minutes!
AMRESCO’s ZiP™ Reversible Protein Detection Kit allows for visualization
of proteins in a polyacrylamide gel that is fast and completely reversible.
5µl of AMRESCO wide rangeprotein marker (Code: K494)was run in each well of a 10%NEXT GELTM and stained usingthe ZIPTM Reversible ProteinDetection Kit. The gel imagewas documented by scanningon a flatbed scanner with a darkbackground placed over top.
l Allows for the obtaining of sequence
data from proteins not detected on
transfer membranesl Offers an established method of
detection when structural analysis is
requiredl Provides a completely reversible
detection method compatible with
most downstream applicationsl Easy to use protocol
Application:
Protein detection
Sequencing
Mass spectrometry
Western blotting
2X PCR MASTER MIXFast and efficient PCR: simply add DNA template and primers
Fermentas 2X PCR Master Mix is a premixed 2X concentrated solution of
Taq DNA polymerase, PCR buffer, MgCl2 and dNTPs. The mixture is optimized
for consistent and efficient routine PCR amplifications.
Features:l Convenient: single tube, single pipetting step.l Ready-to-use: simply add DNA template and primers.l Scaleable: sufficient for 100 standard PCR reactions of 50µl volume: can
be scaled down or up as required.l Pre-aliquoted into separate vials: low contamination risk.l Comprehensive protocol included.
2X PCR Master Mix Composition:l Taq DNA polymerase (recombinant) in Reaction Buffer: 0.05u/µll MgCl2: 4mMl dNTPs (dATP, dCTP, dGTP, dTTP): 0.4mM of each.
Stability of 2X PCR MasterMix. Amplification of 950 bpsingle copy gene from 0.1µghuman genomic DNA using2X PCR Master Mix in 50µlreaction mixture.M - GeneRuler™ 100bp DNALadder Plus1 - Classical PCR with 1.25uTaq DNA Polymerase2 - 2X PCR Master Mix (-20°C)3 - 2X PCR Master Mix (after15 freeze-thaw cycles)
Features:l Fast - disruption of up to 192 samples in as little as 2-4 minutesl Cross-contamination-free - closed system prevents tube-to-tube
carryoverl Reproducible - highly standardized disruption methodsl Flexible - compatible with different sample types and disruption
buffersl Integrated - part of QIAGEN’s tissue-management system from
collection/stabilization to purification
Applications
The TissueLyser is well suited for high-throughput processing of animal
tissues. This is in contrast to rotor–stator homogenization, where samples
must be processed individually, and the rotor–stator homogenizer must
be cleaned after each sample to prevent cross-contamination.
Rat liver, lung, spleen, and kidney tissue was stabilized inRNAlater RNA Stabilization Reagent for 1 year at –20°C andthen processed in lysis buffer using theTissueLyser. TotalRNA was purified using the RNeasy Mini Kit and analyzedby formaldehyde agarose electrophoresis.
TissueLyser SystemFor high-throughput disruption of awide range of biological samples
TissueLyser withTissueLyser AdapterSet (96-well)
TissueLyser AdapterSet (24-tube)
Liver Lung Spleen Kidney
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January 2006, Volume - 1
A Three in one kitTRI Reagent® Now available from Ambion
This is a complete, ready-to-use reagent for the isolation of total
RNA or the simultaneous isolation of RNA, DNA, and protein from
a variety of biological samples. Starting material can be either fresh
or frozen tissue, or cell samples of almost any size. It is an
economical alternative to the use of nucleic acid isolation kits.
l Simple—A single reagent for isolation of DNA-free RNA, RNA-
free DNA, and proteinl Productive—Obtain higher yields than with traditional
guanidine thiocyanate or cesium chloride methodsl Versatile—Works with many sample sources, including
human, plant, yeast, bacterial, and viral samplesl Flexible—Easily scalable depending on starting material and
desired yieldl Efficient—Samples processed in about 1 hour
Starting Material RNA Yield
Liver tissue 6-10 µg/mg tissue
Spleen tissue 6-10 µg/mg tissue
Kidney tissue 3-4 µg/mg tissue
Skeletal muscle or brain tissue 1-1.5 µg/mg tissue
Placenta 1-4 µg/mg tissue
Cultured epithelial cells 8-15 µg/106 cells
Fibroblasts 5-7 µg/106 cells
Target Preparation for Affymetrix GeneChip Analysis
New!—MessageAmp™ II-Biotin Enhanced Single
Round aRNA Amplification Kit
Features/Benefits
l Detect up to 9% more genes due to increase in Percent Present Calls
l Up to 10,000 fold amplification from just a single round
l Single round of amplification from as little as 50 ng of input RNA
l Optimized NTP mix containing biotinylated UTP reduces set up time and
maximizes labeling
l Highly reproducible results
l Reduce reaction costs by as much as 45%
Optimized IVT reaction, which includes, Bio-11-UTP in a new NTP mixes
l Obtain sufficient amount of amplified RNA from a single round of
amplification with only 50 ng of input RNA
l Biotinylated nucleotide included in the optimized NTP mix, reducing set
up time and maximizing labeling
l MegaScript® IVT reaction ensures optimal representation of the gene
expression profile
Higher Yields from as Little as 50 ng Input RNA
Optimization of the IVT step of the MessageAmpII-Biotin Kit protocol produced significantly higheryields after both a 14 h (overnight) and 4 h IVTreaction (single round of amplification) than thoseobtained with Company A’s system. Amplificationof just 50 ng of input RNA after a 14 hr IVTreaction yields enough cRNA (aRNA) for use onan Affymetrix GeneChip®. All reactions wereperformed in triplicate.
Downstream application:
RT-PCR, amplification for array analysis,
hybridization assays, or in vitro translation.
PCR, Southern blotting, and cloning. Isolated
proteins can be used in SDS-PAGE and
Western blots.
50 ng
Input
Single Round
Amplification
GeneChip
Analysis
aRNA Yield from Nine Different Sources. Four different amount of totalRNA from nine different sample types were amplified using theMessageAmpTM II-Biotin Enhanced Kit (Cat# 1791). Average aRNA yieldfrom triplicate reactions are shown for 4 or 14 hr in vitro transcription (IVT)reactions. Note that there is a ~3-fold difference in aRNA yield betweensome samples. With most RNA sources. 50-100 ng of input total RNAamplified with the MassageAmp II-Biotin Enhanced Kit using a 14 hr IVTincubation will yield enough labeled aRNA for microarray hybridization
What’s the Difference?
The MessageAmp II-Biotin Enhanced Kit
incorporates several improvements to the
in vitro transcription (IVT) step of the
MessageAmp II protocol. First the IVT
reaction has been optimized for the highest
yield of biotinylated amplified RNA (aRNA).
The most significant enhancement is
observed at the 50-100 ng input level where
you can obtain enough aRNA for a
GeneChip hybridization after one round of
amplification. This is a 20-50% increase in
yield over MessageAmp II.
Also for high yields, ease-of-use, and cost
savings, a biotinylated nucleotide has been
incorporated into an optimized NTP mix
for the new MessageAmp II Biotin
Enchanced kit.
V
RecoverAll™ Total Nucleic Acid
Isolation Kit for FFPEl Optimized for isolation of total nucleic acids, including microRNAs,
from FFPE tissuel No overnight Proteinase K digestion required—deparaffinize in
the morning and perform qRT-PCR in the afternoonl Obtain typical yields of >50% that of unfixed tissue from the same
sample sourcel Recovered nucleic acids are suitable for qRT-PCR, qPCR, mutation
screening, and microarray analysis
Deparaffinize in the Morning, Perform qRT-PCR in the Afternoon
Maximize Yield from Archived and Freshly Fixed Tissue
Gene Expression from FFPE Samples
Isolating DNA from FFPE Samples
Obtain microRNA for Expression Profiling
Yield of RNA from Archived Human FFPE TissueSamples: RecoverAll™ vs. Two Competitor SystemsA 10–20 µm section from each of the abovearchived human tissue blocks was isolated usingeach of three kits: Competitor A (red), Competitor Bkit (blue), and Ambion's RecoverAll Kit (green).Colon, lung, and breast samples were 1–2 yearsold; kidney was 3–5 years old; and bladder was 10–15 years old. Once the RNA was isolated, theconcentration was determined via OD260, and theamount of RNA recovered in µg was calculated. TheRecoverAll Kit yielded the highest recovery of thethree systems for all
Cells-to-Signal™ KitFrom Cells to qRT-PCR in just 5
minutes!
Save time and money with the rapid,
room temp. lysis procedurer Quantitate gene expression in cells — without isolating RNA
r 5 minute cell lysis procedure
r qRT-PCR from as few as 3 cells
r Ideal for monitoring siRNA-induced knockdown
r Easily adapted to high throughput analysis
The Cells-to-Signal Kits contain reagents for 30 or 100 reverse
transcription reactions, including M-MLV RT, oligo(dT)18 primers,
and random decamer primers. Up to 5 x 104 cells per reaction
can be used for the lysis step; 30% of the lysate can then be
used in the RT reaction.
qRT-PCR Detection of GSK-3α Using TaqMan® GeneExpression Assays and Following Cells-to-Signal™ ProtocolHeLa cells were lysed at concentrations of 1000, 200, 40, 8,and 1.6 cells/µl according to the Cells-to-Signal lysisprocedure. 6 µl of cell lysate were used in a 20 µl one-stepRT-PCR using a TaqMan Gene Expression Assay specific forGSK-3α.
LeukoLOCK™ Total RNA Isolation System
The LeukoLOCK™ Total RNA Isolation System is an innovative method for fractionation, stabilization, and extraction
of total RNA from the leukocyte population of whole blood. The LeukoLOCK System incorporates a leukocyte
depletion filter technology to isolate leukocytes from whole blood and Ambion’s RNAlater to stabilize the cells on
the filter. The RNA that is purified from captured leukocytes is inherently depleted of globin mRNA, improving its
utility for expression profiling and other applications
Features and Benefitsl A twenty fold reduction in absolute levels of alpha and beta globin mRNAsl Stabilization of the global expression profilel Eliminates the need to do an additional globin reduction protocol (enzymatic or otherwise)l Provides high quality RNA (1.8-2.1 A260/280 ratios)l Obtain an increase in percent present calls compared to whole bloodl Gives yields of 10-20 µg per 9 ml of blood
Application
RNA isolated from whole blood using the LeukoLOCK system is suitable for both end point and real time RT-PCR as
well as other downstream applications such as Northern blots, and ribonuclease protection assays. In addition,
highly reproducible results after amplification and array analysis were obtained from RNA isolated using the
LeukoLOCK system.
Ambions first kit for purifying WBC populations
Total RNA was isolated from whole blood using the LeukoLOCKSystem. Fractionated WBCs were processed either immediately(Day 0) or after 3 days of storage on the LeukoLOCK filter inRNAlater. The RNA was analyzed in real-time one-step RT-PCRusing MessageSensor RT conditions. Note the increase in Ctvalues presented for globin targets compared to whole blood, andthe stability of the Ct values for the remaining targets in Day 0 vsDay 3 RNA.
Improve Expression Profiles from Blood
GLOBINclear™– mRNA Removal from
Human, Mouse & Rat Samples
GLOBINclearTM Systeml Remove up to 95% of globin mRNA from whole blood total RNAl Detect up to 50% more genes—analyze previously undetected genesl Achieve greater representation of the genome, low 3'/5' ratiosl Avoid RNase H treatments that can degrade mRNA and alter expression profilesl Incorporate procedure with any RNA labeling protocol, including Ambion’s MessageAmp™ II aRNA Amplification
System
It is widely known that expression array data generated from whole blood total RNA samples have reduced detection
sensitivity compared to data from fractionated blood samples. Examination of whole blood total RNA reveals a
Increased Sensitivity with GLOBINclear™-Mouse/RatTriplicate GLOBINclear-Mouse/Rat reactions were performedwith pooled total RNA samples derived from either mouse orrat blood. The processed RNA was then amplified withMessageAmp II-96 to synthesize biotinylated aRNA probe forGeneChip® array analysis. Biotinylated aRNA was thenhybridized to triplicate Affymetrix microarrays (Mouse430A_2or Rat230_2 GeneChips). GLOBINclear processing resulted inan increase in genes, 46% & 56% for rat and mouserespectively.
dominant band in the 600-700 base region. This band
represents globin mRNA, which is expressed at high
levels in red blood cells (RBCs) and reticulocytes (RBC
precursors). Up to 70% of the mRNA (by mass) in whole
blood total RNA is globin transcripts.
Ambion offers a solution to this problem with
GLOBINclear. The GLOBINclear System employs a
novel, non-enzymatic technology that rapidly depletes
up to 95% of the alpha and beta globin mRNA from
total RNA preparations derived from whole blood. The
kit employs a novel hybridization technology that takes
advantage of the strength of biotin/streptavidin binding,
the specificity of nucleic acid hybridization, and the
convenience of magnetic separations
DDNA labeling using Biotin
ULS® reagent. The ULS®reagent binds to N7 of purineresidues to provide a stablecoordination complex betweenthe nucleic acid and the label(biotin or fluorescein).
Sensitivity of detection of labeled DNALabeled lambda/HindIII was used as a hybridization probe in a dot blot ofhomologous DNA (Nylon membrane)a- labeling with Fluorescein ULS Labelling kit, detection with Anti-fluocein-
AP, Fab fragments in combination with the chromogenic detection (NBT/BCIP)
b- labeling with Biotin ULS Labelling kit, detection with Straptavidin-Alkalinephosphate in combination with the chromogenic detection (NBT/BCIP).
Biotin ULS® LABELING and FLUORESCEIN
ULS LABELING KITS :Non-radioactive chemicals labeling of nucleic acids. It uses the Universal Linkage System (ULS®) technique,
which is based on the stable coordinative binding of a platinum complex to nucleic acids. The platinum complex
acts as a linker between a detectable marker (label) molecule, i.e. fluorescein or biotin, and DNA or RNA.
Featuresl One-step reaction.l Fast - only 30min to label the target.l Universal - any nucleic acid, independent of size or structure can be labeled.l Easy to scale up and down. It allows labeling of as little as 25ng or as much as 10µg of nucleic acid in a
single reaction.
Applicationsl For non-radioactive, non-enzymatic direct chemical labeling
of any nucleic acid with biotin (Biotin ULS® Labeling Kit) or
fluorescein (Fluorescein ULS® Labeling Kit):l Single-stranded and double-stranded DNA;l linear or supercoiled DNAs;l oligonucleotides;
l PCR products;
l chromosomes,
l DNA/RNA markers, RNA, PNA;
l siRNA.
l Labeled nucleic acids can be used in:
l spot blot analysis;
l dot blot hybridization;
l Southern blot analysis;
l Northern blot analysis;
l in situ hybridizations;
l comparative genomic hybridization.
NEW
NEW
NEW
The LeukoLockTM kit seperated into two parts-one containing theStabilization and Seperation reagents and the other containing the Isolationreagents. We are offering the following kits in addition to the origional kits.The LeukoLOCKTM Total RNA Isolation Kit Cat. No. 1933
The LeukoLOCKTM Fractionation and Stabilization Kit Cat. No. 1934
NEW
NEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:49 PM5
6
January 2006, Volume - 1
Tosoh BioscienceBiochromotography Scenario
TSK-GEL 5PWprepacked columns
TSK-GEL 5PW Bulksemi-prep. scale
(20, 30 µm av. particle size)
Toyopearl prep(35, 65, 100, 200 µm av.
particle size)
Application support Application and registrationsupport
registration support
continous selectivity for easy scale up
Operating Volume 2 - 200 ml
Column ID 5 - 20 mm
Column length 40 + 20 cm
1 - 50 I
100 - 200 mm
40 + 20 cm
100 - 500 I
> 400 mm
40 + 20 cm
Leading provider and manufacturer of diagnostic test
components for detection of
SARS, HIV, HCV, HBV, HAV,
HDV, Syphilis and other
infectious agents.
NEW Products from VIROGENanti-Glutathione mAb D3
anti-GSK3B mAb 1H8
anti-FAS mAb 5F7
anti-FAS IgM mAb IPO-4
Induces apoptosis better then CH11
anti-CDw150 mAb IPO-3
anti-CD37 mAb IPO-24
anti-p53 mAb BP53-12
anti-CD3 mAb RIV9
anti-CD8 mAb RIV11
anti-CD16 mAb ASH 1975
anti-CD20 mAb B9E9
anti-CD22 mAb MYG13
Spectra/Por® dialysis tubing from
Spectrum Laboratories®.Spectra/Por® dialysis tubing is made of either regenerated cellulose
(RC) or cellulose ester (CE). Cellulose has long been used for dialysis
as it is uncharged and does not readily
absorb solutes. Further, the selectivity of
cellulose membranes is not altered greatly
by many chemicals or reasonable pH and
temperature ranges. Processed cellulose
has crystalline regions and these regions
cross-link chains to introduce structural
integrity to the cellulose. Depending upon
how the cellulose is processed the number
of crystalline areas varies and the resultant
regions between the cross-links can act
like size-selective pores.
Many Spectra/Por® membranes are
available with molecular weight cut offs
(MWCO) from as little as 0.1 kDa all the way up to 300 kDa (CE
membranes come in 0.1, 0.5, 1, 10, 100 and
300 kDa MWCOs, basic RC membranes
come in 3.5, 6–8 and 12–14 kDa MWCOs and
more expensive RC membranes have a range
of MWCOs from 1–50 kDa). MWCO values
are established by Spectrum® and represent
the size at which a solute is 90 % retain during
a test period.
Spectra/Por®
RC Dialysis Membrane
Spectra/Por®
CE-Membrane
Spectra/Por® 2
Dialysis Membrane
MWCO-12,000 - 14,000
Spectra/Por®
Membrane Tubing
Spectra/Por® is
supplied in an
essentially ready-to-go
format, no boiling in
bicarbonate buffer is
needed. Spectra/Por®
1–6 merely need
soaking in distilled
water for 30 minutes
whereas other tubing is
supplied pre-hydrated
vAntibodiesv Apoptosisv Signal Transductionv Cyclooxygenasev Endocrinologyv Enzyme Immunoassayv Leukotrienesv Lipids Lipoxygenasev Neurochemistry
Cayman serves you infollowing areas
RNA Research Essentials!
RNAlater® Tissue Collection: RNA
Stabilization Solution
3 Simplifies sample collection -
one reagent immediately
inactivates RNases and
stabilizes RNA within tissues or
cells
3 More flexibility - no need to freeze
samples in liquid nitrogen or rush
samples back to the lab freezer
3 Eliminates the need to freeze and
grind most tissue samples
3 Perfect for tissue collection "in
the field"
3 Flexible tissue storage - RNA is
stable for 1 day at 37°C, 1 week
at 25°C, 1 month at 4°C or
indefinitely at -20°C
3 Compatible with many RNA
isolation procedures including all
of Ambion's RNA isolation kits
RNaseZap® RNase Decontamina-tion Solution
3 Completely removes RNase
contamination from glass and
plastic surfaces
3 RNaseZap excels at removing
high levels of RNase
contamination whereas similar
products fail
3 Proven effective at removing high
concentrations of dried-on
RNase A
3 Ideal for cleaning work surfaces,
pipetters, and equipment that
must be RNase-free
RNAlater® Tissue Collection: RNAStabilization Solution
RNase-free Tubes and Tips
3 Tubes have a positive seal design, withstands -90° to 120°C
3 Available in a variety of sizes
3 Non-stick microfuge tubes are available with a low binding surface
3 PCR Tubes are functionally tested by PCR
Now mailing tissue samples
from collection site is possible
Removes RNase contamina-
tion from glass and plastic
surfaces without autoclavingGuaranteed RNase- and DNase-free
New! 15 ml and 50 ml Conical Tubes
Ambion’s RNase-free Conical Tubes are provided assembled with green
screw caps. The tubes have a printed writing space and volume marks
on the sidewalls for convenience. These polypropylene tubes are sterile,
nuclease-free, nonpyrogenic, and can be autoclaved to 121°C.
Microfuge Tubes
RNase-free microfuge tubes are available in three sizes, and each is
marked with convenient graduations. Nonstick RNase-free tubes are
useful for handling low concentrations of RNA and/or DNA because they
minimize nonspecific binding to the tube.
Elution Tubes
These 2 ml tubes can accommodate RNAqueous® and
Poly(A)Purist•Espin cartridges.
Dome Cap PCR Tubes. The thin-walled, dome-cap PCR tubes allow
uniform contact with the thermal cycler temperature block resulting in
superior well-to-tube temperature transfer and rapid cycle times.
Frosted Lid PCR Tubes
The thin-walled, flat, frosted cap provides a suitable surface for detailed
labeling.
Barrier Tips
Ambion's barrier tips prevent cross-contamination of samples by means
of an aerosol filter. They are RNase- and DNase-free and are ideal for
handling RNA, PCR applications, and for working with radioisotopes or
toxic chemicals.
Tips are compatible with Eppendorf®, Finnpipet, Oxford Benchmate®,
Pipetman•E Proline®, Rainin, and Socorex pipettors.
3 DEPC and non-DEPC treated water
3 High quality
All water products are certified to be nuclease free.DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation ofDEPC; available in 100 ml, 500 ml and 1 liter sizes.
Certified nu-
clease free-water
is also available
with Ambion
Cat# 9930
Tubes have either
dome caps (0.5
ml size) or frosted
lids (0.2 and 0.5
ml sizes).
Tubes can
accomodate
RNAqueous®
spin cartridges.
15 ml and 50 ml
Conical Tubes
Marked with
graduations and
are available in
three sizes.
Blue-BANDit™
• No More Methanol and Acetic Acid
• Sensitivity Below 20 ng of Protein/Band
• Hands-Off! Simple Staining/Destaining
Procedure
AMRESCO’s Blue-BANDit™ stain is a conve-nient alternative
to traditional Coomassie® Blue staining procedures.
Staining results are clear and precise. Environmentally
friendly, this ready-to-use stain does not contain methanol
and ace-tic acid and does not require hazardous sol-vents
for destaining. Blue-BANDit™ staining exhibits sensitivity
below 20 ng of protein per band. Packaged as a 1X ready-
to-use solution, Blue-BANDit™ only requires water for the
prewashing and destaining steps.
PHOTO LEGEND: Results of an 8%
polyacryla-mide gel loaded with
serial dilutions of High Range Protein
Marker (40-212 kDa) using 1X TG-
SDS buffer and visualized with Blue-
BANDit TM (Gel A) and Standard
Coomassie® Blue G-250 (Gel B).
v Nitric Oxidev Nuclear Receptorsv Oxidative Injuryv Oxylipinsv Phospholipasev Photo Detectionv Prostaglandinsv Fatty Acidsv Steroids
and must be stored at 4 °C. One concern is that these membranes contain
trace amounts of heavy metals and sulfur although Spectrum® do also
produce specific cleaning solutions and supply Spectra/Por® 7 which
comes with minimal contaminants. Membranes can be sterilized and
also have two year shelf lives. Finally, Spectra/Por® tubing is supplied in
different widths depending upon the scale of dialysis you plan.
l Easy to usel Stable Surface chemistryl Compatible with automated microarray equipmentsl Low background fluorescencel Polymer slide for protein microarrays Ideal where very low
concentrations are to be quantifiedl Low background fluorescencel Shelf life 5 years Improved signal to noise ratio versus glass slidesl Batch to Batch Consistancy
Protein MicroArray SlidesThese slides are specifically desinded for protein
microarray. The protein molecules are bound to the
surface by a stable, non-covalent interaction. It is
not necessary to perform any protein modification
steps before using the slides. The MaxiSorp surface
is applicable for many protein array, such as
antibody,antigen and peptide assays
Barcoded
slides available
on request
NEW
96 CytoWell™ Plates
for cell based assayOptical Bottom
l Black cell culture plates with transparentbottoms.
l Excellent optical quality.l Thin 50 µm film bottoms for high.
magnification and resolution microscopyand imaging.
l Flat bottoms across the plates facilitateautomatic imaging.
l Flatness conforms with requirements forconfocal microscopy.
l High gas transfer rates facilitate cellculture of fastidious cells.
l Surface treatment ensures uniform, stablecell attachment and growth.
l Very low auto fluorescence.l UV-transparent.l 96 well footprint compatible with standard
equipment and automated systems.
Robust high performance plates
for automated handling and imaging
Also available in 384 Well Plate
format from January 2006
Literature Available on Request
1 Lineage tracing and Charactrization of
insulin-secreting cells guarated from
adult pancreatic aciner cells.
1 Large-Scale Production of Recombinant
Viruses by Use of a Large Culture
Vessels with Active Gassing.
NEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM6
7
January 2006, Volume - 1
Vivapure - the next step in protein purificationVivascience Protein Purification The separation of pure proteins from complex mixtures is a key process in biomedical research and other biological disciplines. Vivascience offers Vivapure spin
columns based on an innovative and powerful membrane adsorber technology for the purification of proteins.
Vivapure AdenoPACK™ 500 - for Adenovirus
purification and concentration
Kit for adenovirus purification and concentration : In only 2 hours 3 x 1013 purified virus particles are recovered
from 500 ml cell culture. This easy to use kit replaces lengthy and inefficient cesium chloride density gradient
methods and is the most cost-effective kit in the market for purifying 3 x 1013 viral particles
Handling overview - Purified virus in about 2 hours
Add Benzonase
and clarify viralsupernatant
Load clearedviral supernatant
Wash Elute Concentrate
Membrane adsorber technology:
Membrane adsorbers are ideal for virus purification. Large flow
through pores allows unrestricted adenovirus access and
convective transport speeds purification.
Fluorescent cell assay
Photography: kindly provided by Dr. Lux, University Hospital and
University of Applied Sciences, Mannheim. COS-1 cells infected
with Ad5 GFP-constructs after purification and concentration
with Vivapure AdenoPACK 100.
The Vivapure Advantage
l Rapid 2 hour purification protocol
l Fast recovery of 1 - 5 x 1012 VP from 500 ml cell culture
l Easy to use
l Non-toxic buffers
l Fast
l Cost effective
l Centrifuge based
l Parallel processing
l Reproducible
Available in a range of different membrane chemistries
(e.g. range of strong or weak ion exchangers, metal chelate, Protein A, epoxy)
Vivapure Anti-HSA/IgG Kit for Human Albumin and IgG Depletion
Highly specific human albumin and IgG depletion with unique antibody fragments and Protein G
Reveal your proteome - see previously undetectable proteins
Handling overview - Albumin and Albumin/igG removal in 20 minutes
Fill Vivaclearwith Anti-HSA.IgG
Affinity Resin
Mix. sample with Anti-HSA/igG Affinity Resin
incubate for 15 min.
Recover albuminand igG depleted
sample
Wash 1 - 3x with BindingBuffer for
complete samplerecovery
Albumin and IgGdepleted sample isready for analysis
l Highly specific antibody fragments >95% albumin removal from human serum
l No cross reactivity with other human proteins
l Compatible with all downstream proteomics applications due to low salt
concentrations in the buffer
l Fast 20 min. protocol
l Easy-to-use spin column based protocol for parallel processing
l Flexibility in sample volume to be depleted
Human serum was analysed before and after albumin/IgG removal by IEF and 2D PAGE. The comparison of the two
gels clearly demonstrates the increased resolution, leading to the visualisation of lower abundance proteins achieved
with the Vivapure Anti-HSA /IgG Kit.
Vivapure Anti-HSA/IgG Kit Advantages
Antibody based albumin removal Efficient albumin removal with minimal loss of
potential serum biomarkers
Protein G based IgG removal Efficient IgG removal. Also applicable to mouse and
rat samples
20 minute protocol employing spin tubes Quick, suitable for parallel processing
Depleted protein recovered in low salt buffer Sample is ready for next step in fractionation process
Resin packaged as a slurry Flexibility in serum sample volumes
Kit format Ready to use, no wastage
Priced for single use No risk of cross contamination
New&
Improved
Vivapure® C18 Micro spin columns - Improved sample Newpreparation for mass spectrometry:
It contain an innovative C18 membrane and are easily handled in the centrifuge. They are ideally suited for
concentration, purification and desalting of peptides prior to analysis.
Vivapure® C18 - Benefits
l Easy centrifugal procedure
l Minimal hands on time
l High loading capacity (up to 200 µl)
l Low elution volume (3 µl)
l Direct elution with matrix possible
l Highly reproducible
l Convenient parallel processing
Desalting and sample concentration with Vivapure® C18 Micro spin columns gave rise
to a better resolution and an increased number of signals suitable for protein dentification
Handling overview
Equilibrate Bind Wash Elute Purified sample
New
Vivapure Epoxy Protein Coupling Kit - Create
your own affinity matrix for purifying your desired
protein
Coupling affinity ligands with epoxy groups
The Vivapure Epoxy Protein Coupling Kit is a new tool for protein
immobilization for creating any desired affinity spin column. This ready-to-use
kit utilizes an epoxy membrane adsorber that is fitted into a spin column for easy
and quick handling
Immobilization of proteins. Amino acids possessing either amino-, hydroxyl- or
thiol-groups can be immobilized with the epoxy group.
The Vivapure Advantagel Fast results - only 3 hours couplingl Mild coupling conditionsl Ready-to-use kit
Handling overview
CustomProteinSample
Dilute 1:1withBuffer A
EquilibrateMembrane
LoadDiluteProteinsample
flow-through
Pipetteflowthroughontomembrane;incubate 3hr at RT
Wash Customproteincoupled toVivapureEpoxy MiniSpin Column
New
Vivacell 100 utilizes:l Pressurel Centrifugel Pressure-shake
Vivacell 100
20 - 100 ml samples
Vivacell 100 is the latest member of the Vivacell family and bridges the volume range between
the Vivacell 70 and the Vivacell 250. The patented vertical membrane design allows highest
performance and unmatched flexibility. Vivacell 100 is a unique and
innovative concentrator for volumes from 20 ml to 100 ml, which
utilizes pressure, centrifuge or pressure-shake to rapidly concentrate
even samples with very high particle loading. Vivacell 100 is designed
for centrifugal concentration of samples up to 100 ml which makes
it the largest centrifugal unit available. At the same time, the new
construction design allows for maximum centrifugal force of 4,000xg
to be used for even faster concentration.
Vivaspin 15R
2 - 15 ml samples
Vivaspin 15R is the latest member of the Vivaspin product family with all
the unique features of Vivascience concentrators ncluding a patented
vertical membrane and a dead stop. Vivaspin 15R is targeting the volume
segment 2 to 15 ml with a modified regenerated cellulose membrane;
Hydrosart®. This membrane is ideal where extremely high recovery with
very low adsorption is needed, for example in applications such as desalting
and concentration of Ig fractions.
l Ultimate recovery at low Adsorption (95-98%)
l Extremely short concentration time (30x in 15 min.)
l Convenient application protocol with easy handling
l Easy scale-up to Vivaflow 200 with Hydrosart® membrane for
volumes up to 5 litres
l Very small hold up volume (< 20 µl)
Spin Recover
Direct
upscale of
AdenoPACK 100
NEW
NEW
NEW
NEW
NEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM7
8
January 2006, Volume - 1
Etest® trademark now enjoys internationalrecognition and It is the first in a new generationof novel and patented products for Anti-microbialResistance Testing.Etest comprises a predefined gradient ofantibiotic concentrations on a plastic strip.It is used to determine the exact MinimumInhibitory Concentration (MIC) of antibiotics,antifungal agents and antimycobacterial agents,in µµµµµg/ml.It is a cost-effective tool for generating MICsacross 15 dilutions. It can also be used in amacromethod format to optimise resistancedetection. Over 100 anti-biotics are nowavailable in the product range for testing of
aerobic bacteria and fastidious organisms. Etest promotes the rationaluse of antibiotics by providing accurate results to guide the therapy ofindividual patients and to validate empiric drug regimens on a unit specificbasis. It is also widely used in resistance surveillances and clinical trials.
Media for the antibiotic testing varies fromorganism to organism
Configuration of Epsilometer
The Etest comprises of a thin impervious test car-
rier (5x50) with a continuous exponential gradi-
ent of antibiotics immobilized on one side and a
reading and interpretive scale on the other side.
The antibiotic gradient can cover a broad con-
centration range
corresponding to
approximately 20
MIC two-fold dilu-
tions. The slopes
and concentration
ranges can be op-
timally designed to
correspond to clini-
cally relevant MIC
ranges and break-
points selected for
categorization of
s u s c e p t i b i l i t y
groups.
Epsilometer test Principle: The antibiotic gradi-
ent is applied onto the surface of an inoculated
agar plate. After the required period of incuba-
tion whereby bacterial growth becomes visible an
inhibition ellipse is seen. The zone edge intersects
the lenth of the graded carrier at a position where
a specific concentration of the antibiotic causes
an inhibition and cessation of bacterial growth.
The value named the inhibitory concentration (IC)
is a direct measure of the susceptibility of the
micro organism for the particular antibiotic.
Storage and Handling of Etest Strips
1. Etest strips are supplied in a plastic blister pack-
age with 10 units in
each of 10 individu-
ally sealed com-
partments. Each
package contains
100 units of one an-
tibiotic.
2. All packages, in-
cluding unopened blister compartments, must
be stored in a freezer at -20°C until the expiry
date.
3. Always store unused strips in airtight contain-
ers e.g. storage tube with silica gel. Ensure that
the desiccant is blue during storage and be-
fore use.
4. Reactivate the desiccant when it turns light blue
or white, remove the cap, replace the
Styrofoam with a plug of cotton wool and heat
the tube to 160oC for
one hr. cool the tube,
replace the cap and
store in dry place.
5. Mark the storage
tube with the label
from the blister
pack,showing the expiry date,batch no and
antibiotic code.
6. Store the tube at –20oc and always allow it to
reach room temprature before opening.
7. If stored at –70oc,leave the tubes in the
Styrofoam outer packaging during equilibra-
tion to room temprature to prevent the caps
from popping out of the tubes.
8. Strips in airtight storage tubes have the same
expiry date as the origional package, if prop-
AB BIODISK develops new product concepts and processes for manufacturing unique high quality diagnostic tests. The company’sproprietary technology enables biochemical reagents to be accurately dispensed in picogram quantities in stable dry-chemistryformats. This innovative technique is used to produce antibiotic gradients for products like Etest®, System.
erly stored.handled
and always kept dry.
Application
Open the package us-
ing forceps or your fin-
gers, remove the re-
quired number of Etest
strips and place them on a dry clean surface e.g.
a petri-dish or in the Etest applicator tray Use a
manual applicator, vacuum pen or forceps to ap-
ply the strip to the inoculated agar surface with
the MIC scale facing upwards (towards the
opening of the plate) and the handle at the rim of
the plate. Ensure the whole strip is in complete
contact with the agar surface. Do not place the
strip upside down as no inhibition ellipse will form
because the antibiotic
will not diffuse across
the non-porous plastic
strip. If air pockets are
seen under the strip, re-
move them by pressing
gently on the strip (with-
out moving it) with forceps or an applicator stick
moving from the low concentration upwards.
Small bubbles will not affect results. Once ap-
plied, the strip cannot be moved because of
instantaneous release of antibiotic into the
agar.
Epsilometer test procedure: The procedure for
using the Etest for antimicrobial susceptibility test-
ing consists of three major steps.
An agar plate with a suitable test medium is in-
oculated according to standard practice. This may
include flooding swabbing or seeding the test
plate with the microorganism. The inoculum used
may be a suspension of an overnight culture, the
so-called ICS inoculum, or a 0.5 McFarland sus-
pension of a log phase culture. Test carriers are
then applied in an optimal pattern as shown in fig
3 with the concentration maxima nearest the cir-
cumference of the petridish. The plate is immedi-
ately incubated in the required atmosphere for
15-18hrs. After incubation inhibitory concentra-
tion are directly read from the graded test carri-
ers. Results can be reported as a quantitative
value, i.e. an inhibitory concentration of x µg/ml
and or a susceptibility group as defined by spe-
cific MIC/IC correlated break points.
Product µµµµµg/ml Cat No.
Amikacin 0.016-256 AB5100 0138
Amoxicillin 0.016-256 AB5100 0098
Amoxicillin/clavulanic acid (2/1) 0.016-256 AB5100 0108
Ampicillin 0.016-256 AB5100 0158
Ampicillin/sulbactam (2/1) 0.016-256 AB5100 0188
Azithromycin 0.016-256 AB5100 0168
Aztreonam 0.016-256 AB5100 0178
Bacitracin 0.016-256 AB5100 2868
Benzylpenicillin (high range) 0.016-256 AB5100 0258
Benzylpenicillin (low range) 0.002-32 AB5100 0268
Cefaclor 0.016-256 AB5100 0458
Cefalexin 0.016-256 AB5100 3018
Cefdinir 0.016-256 AB5100 2888
Cefditoren 0.002-32 AB5100 3448
Cefepime (high range) 0.016-256 AB5100 0508
Cefepime (low range) 0.002-32 AB5100 0498
Cefixime 0.016-256 AB5100 2998
Cefodizime 0.016-256 AB5100 0518
Cefoperazone 0.016-256 AB5100 2958
Cefoperazone/sulbactam (2/1) 0.016-256 AB5100 2938
Cefotaxime (high range) 0.016-256 AB5100 0558
Cefotaxime (low range) 0.002-32 AB5100 0568
Cefotetan 0.016-256 AB5100 0638
Cefoxitin 0.016-256 AB5100 0658
Cefpirome 0.016-256 AB5100 0648
Cefpodoxime 0.016-256 AB5100 0588
Cefprozil 0.016-256 AB5100 2668
Ceftazidime 0.016-256 AB5100 0678
Ceftibuten 0.016-256 AB5100 0718
Ceftizoxime (high range) 0.016-256 AB5100 2658
Ceftizoxime (low range) 0.002-32 AB5100 2738
Ceftriaxone (high range) 0.016-256 AB5100 0668
Ceftriaxone (low range) 0.002-32 AB5100 0708
Cefuroxime 0.016-256 AB5100 0698
Cephalothin 0.016-256 AB5100 0358
Chloramphenicol 0.016-256 AB5100 0758
Ciprofloxacin 0.002-32 AB5100 0868
Product µµµµµg/ml Cat No.Amphotericin B 0.002-32 AB5100 2638
Flucytosine 0.002-32 AB5100 1098
Fluconazole 0.016-256 AB5100 1088
Itraconazole 0.002-32 AB5100 2588
Ketoconazole 0.002-32 AB5100 2598
Voriconazole 0.002-32 AB5100 3288
Etest® For Antifungal
ETEST ESBL FOR CONFORMATION OF EXTENDED SPECTRUM BETA-LACTAMASE
Product µµµµµ/ml Cat. No.
Cefotaxime/cefotaxime + clavulanic acid (4µ/mL) 0.25-16/0.016-1 AB5100 3228Ceftazidime/ceftazidime + clavulanic acid (4µg/mL) 0.5-32/0.064-4 AB5100 3258Cefepime/cefepime + clavulanic acid (4µg/mL) 0.25-16/0.064-4 AB5100 3478ETEST MBL FOR DETECTION OF METALLO BETA-LACTAMASE
Imipenem/imipenem + EDTA 4-256/1-64 AB5100 3428
Etest® For Antimycobacterial
Clarithromycin 0.016-256 AB5100 0878
Clindamycin 0.016-256 AB5100 0958
Colistin 0.064-1024 AB5100 3338
Doxycycline 0.016-256 AB5100 0978
Enrofloxacin 0.002-32 AB5100 2898
Ertapenem 0.002-32 AB5100 3168
Erythromycin 0.016-256 AB5100 1058
Ethambutol 0.016-256 AB5100 2778
Ethionamide 0.016-256 AB5100 2758
Fosfomycin 0.064-1024 AB5100 2918
Fusidic acid 0.016-256 AB5100 1158
Gatifloxacin 0.002-32 AB5100 3028
Gentamicin (high range) 0.064-1024 AB5100 1278
Gentamicin (low range) 0.016-256 AB5100 1258
Imipenem 0.002-32 AB5100 1368
Isoniazide 0.016-256 AB5100 2798
Kanamycin 0.016-256 AB5100 2788
Levofloxacin 0.002-32 AB5100 2748
Linezolid 0.016-256 AB5100 3138
Mecillinam 0.016-256 AB5100 1378
Meropenem 0.002-32 AB5100 1388
Metronidazole 0.016-256 AB5100 3008
Minocycline 0.016-256 AB5100 1608
Moxifloxacin 0.002-32 AB5100 2908
Mupirocin 0.064-1024 AB5100 1638
Nalidixic acid 0.016-256 AB5100 1658
Netilmicin 0.016-256 AB5100 1758
Nitrofurantoin 0.032-512 AB5100 3048
Norfloxacin 0.016-256 AB5100 1958
Ofloxacin 0.002-32 AB5100 1968
Oxacillin 0.016-256 AB5100 2058
Pefloxacin 0.016-256 AB5100 2088
Phenoxymethylpenicillin 0.016-256 AB5100 2138
Piperacillin 0.016-256 AB5100 2158
Piperacillin/tazobactam (4 ¼g/mL) 0.016-256 AB5100 2148
Polymyxin 0.064-1024 AB5100 3348
Quinupristin/dalfopristin 0.002-32 AB5100 2878
Rifampicin (high range) 0.016-256 AB5100 2178
Rifampicin (low range) 0.002-32 AB5100 2608
Roxithromycin 0.016-256 AB5100 2648
Sparfloxacin 0.002-32 AB5100 2578
Spectinomycin 0.064-1024 AB5100 2928
Streptomycin (high range) 0.064-1024 AB5100 2688
Streptomycin (low range) 0.016-256 AB5100 2188
Sulfadiazine 0.064-1024 AB5100 3438
Sulfamethoxazole 0.064-1024 AB5100 3418
Teicoplanin 0.016-256 AB5100 2208
Tetracycline 0.016-256 AB5100 2258
Ticarcillin 0.016-256 AB5100 2238
Ticarcillin/clavulanic acid (2 ¼g/mL) 0.016-256 AB5100 2268
Tobramycin (high range) 0.064-1024 AB5100 3318
Tobramycin (low range) 0.016-256 AB5100 2278
Trimethoprim 0.002-32 AB5100 2368
Trimethoprim/sulfamethoxazole (1/19) 0.002-32 AB5100 2448
Vancomycin 0.016-256 AB5100 2558
Meet with Next Generation Cryo Tubes
FEATURESl The vials are clicked into the low profile micoplate format rack to
allow for automatic manipulation and automated transport without
the risk of loosing samples
l The cap is designed with a socket to allow automated manipulation
through a click of the screwdriver into the cap
l Designed for low temperature storage of biological materials
l Developed for automated handling
l Dense storage format for optimized freezer utilization
l Working volume of 0.5 or 1.0 ml
l CryoBank™ low protein adsorption surface for storage of cells and
proteins
l Bank-It™ for low DNA adsorption surface for storage of DNA
l Unique 14 x 14 array 2D Code
l CE-marked for diagnostic use
l Sterility assurance level 10-6 on the CryoBank vials
l DNAse/RNAse free
l Non-pyrogenic according to the LAL-test (only valid for CryoBank™)
l Non-toxic according to USP class VI test
l Conform to IATA requirements for the transport of diagnostic specimens, UN packing instruction 602 and 650
Innovative Storage Technology forPharmagenomics / Cell Banking / Biobanks
CryoBank™ and Bank-It™
A range of accessories is provided to
facilitate easy handling and storage
Microplate rack
Nunc MicroPlate Storage
Racks in 4 different colours
for storage of CryoBankTM
or Bank-ItTM plates
Storage Box
White cardbord for
169 tubes
Scew Driver
For mannual unscrewing
and fastening of the caps
Handheld electric
scewing heads
The cap is prepared for cap
manipulation with a spring
ball bearing socket
The low binding surface used by Nunc ensures maximum sample recovery
Chose Products
of Your Interest
NEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM8
9
January 2006, Volume - 1
Multidish 4 for IVF Polystyrene. With LidMouse embryo tested Ask for Cat.No 144444
Please observe that the Cat.No 176740 is not mouse embryo testedl Intended use of this 4 well dish is in vitro fertilizationl Same surface, design and packaging as Cat. No. 176740l CE marked according to Medical Devices Directive 93/42/EECl 510K, i.e. produced and marketed in compliance with the FDA
requirements for IVF productsl Full batch control of all components in the final product giving full
traceability
l Easy handling with one hand only Multidish 4 for IVF Nunclon™?
l Same surface, design and packaging as Cat. No. 176740
l CE marked according to Medical Devices Directive 93/42/EEC
l 510K, i.e. produced and marketed in compliance with the FDA
requirements for IVF products
l Full batch control of all components in the final product giving full traceability
l Easy handling with one hand only
l Unique certification based on a 1-cell stage mouse embryo toxicity test; the certificate confirms sterility (SAL 10-
6), non-pyrogenicity and that the material has passed USP class VI test
l The embryo toxicity test is a release test and the product will only be sold after it has passed the test
l Unique certification based on a 1-cell stage mouse embryo toxicity test; the certificate confirms sterility (SAL 10-
6), non-pyrogenicity and that the material has passed USP class VI test
l The embryo toxicity test is a release test and the product will only be sold after it has passed the test
150628
140685
140675
142475
142485
152035
152034
176600
150687
152640
176597
176740
Low Cell Binding Plates and DishesSterile. Polystyrene. With Lid
l Low Cell Binding surface is based on a polymer presenting a phosphorylcholine moiety to the
cells, thereby mimicking the surface of a cell membrane
l Biologically inert and biocompatible
Useful for:
l Culture of oocytes, e.g. Xenopus
l Colony assay for cancer cells
l Isolation of macrophages
l Detection of cytokines and other cell secretions
l Culture of embryonic bodies
l Culture of spheroids, e.g. HepG2
Applications
l Cell Culture
FluoroNunc™/LumiNunc™ Plates Nunclon™Polystyrene. Sterile. External dimensions 128 x 86 mm
l MicroWell™ Plates for fluorescence/ luminescence
based cell assaysl Nunclon™? certifiedl Plate edges and lids are designed to reduce
evaporationl Raised well rims reduce risk of cross contaminationl Fit standard equipmentl White plates for minimum autofluorescence and
autoluminescencel White plates give maximum reflectionl Black plates give minimum background in
fluorescence readingl Black plates allow minimum scattering of light
Applicationsl Cell Cloning Studiesl Cloningl Fluorescence Based Assays
Accessories for
Nunc Cell
Factories
LARGE-SCALE CELL CULTURE PRODUCTIONCell Factories for Active Gassing Nunclon™Length 335 m, width 205 mm
l Accessories for
filling and
emptying the Cell
Factories with
media
l Under a LAF-
Bench, the sterile
funnel may be
used
CELL FACTORY HAND MANIPULATOR FOR CF10 AND CF40l The Nunc Hand Manipulators are designed to manually handle a Cell Factory 10 with a growth area of 6,320
cm 2 respectively a Cell Factory 40 with a growth area of 25,280 cm 2l The CF10 Hand Manipulator comes as a tabletop model with firm base, while
l The CF40 Hand manipulator comes with wheels and a foot brake
BENEFITSl Low initial investment costsl Reduced lifetime operating costsl Reduced validation complexityl Low risk of cross-contaminationl Easy upscalingl Faster to market
EPA-TEC GEN. 9
Automatic Cell
Factory Manipulator
l The AFCM is an electronically and pneumatically
controlled unit which automates the filling and
emptying of medium or cell suspension into 4 x 3
Nunc Cell Factory 10 or 4 x 1 Nunc Cell Factory 40
l Included with the AFCM are 5 assembly racks with
carts and 1 standard set of spare parts
l For industrial scale production, for example
vaccines, monoclonal antibodies or
pharmaceuticalsl The patented Gas-flow system secures a
controlled atmosphere in the culture trays by equal
distribution of user-specified gas mix actively
pumped through the pre-mounted filterl Gas is equally distributed between trays as well
as in each individual trayl For culturing and nursing of cells via controlled
gas distribution - especially oxygen demanding
and pH-sensitive cells may benefit from the
controlled atmospherel Certified Nunclon™? surface treatment ensures
excellent conditions for cell attachment and growthl Growth kinetics unaltered from laboratory scale
culturel Available in 4, 10 and 40 tray versions for easy
scale-up, low contamination risk and Compact
designl Compatible with existing manually and automated
handling equipment from Nunc
Applicationsl Cell Culture, Large Scale Cell Culturel Monoclonal Antibody Productionl Upscaling
EPA-TEC Shakerl Serves to detach cells by performing continuous
horizontal acceleration and deceleration movements
to the Cell Factory/liquid systeml The frequency of the shaking movements can be
controlled through the operating panel. The Shaker
can either be directly controlled or preset by the timerl Built to hold a rack with 4 x 3 Nunc Cell Factory 10 or
4 x 1 Nunc Cell Factory 40
Shaker Length 2300 mmwidth 545 mmHeight 1140 mmWeight 360 kg
Operating Load PowerPanel Shake Timer1
Move1 Timer2, ch. AMove2 Timer2, ch. B
Stop Emergency StopElectric High Voltage AC 400 Volt/0.75KWCabinet Low Voltage AC/DC 24 Volt
Vacuum Pump
A Virtually Two Dimen-
sional.. 2D MicroHex™l Microscopic polystyrene non-porous
hexagons for adherent cell culturel Certified surface treatment ensures ex-
cellent condi-
tions for cell
at tachment
and growthl 2D MicroHex
can be kept
in suspen-
sion at low
stirring speed
due to the shape and low weight of the
particles. This facilitates the initial cell
attachment and results in a uniform cov-
erage of all carriersl 2D MicroHex are solid, non-swelling,
non-porous and non-absorbing par-
ticles. Therefore, no reagents can be
trapped in the carriers, as is the case
with gel particlesl 2D MicroHex have the well-known prop-
erties of Nunc cell culture plastics, in-
cluding easy trypsinization of cellsl Very low displacement volume/unit sur-
face area
Microcarriersfor large scale production
Barcoded CryoTubes™Pad-print/Ink Jet is done by printing directly on the tube
l Withstand temperature changes, like freezing in liquid nitrogen and
the subse-quent thawing
l Standard code Interleaved 2 of 5
l Available from 1.8 to 4.5 ml Label - the unique Nunc label withstands
many chemicals e.g. toluene, acetone and DMSO
l Withstand temperature changes like freezing and thawing
l Resistant to autoclaving
l Available as external and internal thread and from 1.0 to 4.5 ml
l Available with code 128, Interleaved 2 and 5 or code 39
Applications
l Cryogenic Storage
l Gas Phase Freezing
l Liquid Nitrogen Freezing
l Mechanical Freezing
l Sample Identification
NUNCVarious Competitors
L929 Cell Adsoption to CryoTube (3 days incubation)
NUNC V/s Competitors
NEW
NEW
NEWNEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM9
10
January 2006, Volume - 1
Streptavidin Passively Coated Plate and Module96 wells per frame. Polystyrene. External dimensions 128 x 86 mm
l Ideal for binding of biotinylated biomolecules, such as peptides, antibodies, oligonucleotides or haptens
l Streptavidin is passively coated on an area of 154 mm² (area covered by a volume of 200 µl)
l Binding capacity for biotin of at least 13 pmol/well*
l Stable at room temperature
l General coating protocol available on request
l Available in C96 plates or C8 well strips
Applications
l Covalent Immobilisation, ELISA
* Depending on size or sterical properties of a given biomolecule the actual moler binding
capacity might be different
Immobilizer™ Plates and
Modules Streptavidinl Instant coupling of biotinylated bio-moleculesl Streptavidin is covalently bound to the surfacel Ready to use - no blocking stepsl Excellent binding capacity (e.g. F96 clear 20 pmol/
well*)l Minimal surface leachingl Reaction site easily accessiblel Low non-specific bindingl High signal to noise ratiol Long shelf-life at room temperature (minimum 18
months)l Low reagent consumptionl White and black plates for luminescence or
fluorescence applications
l Depending on size or sterical properties of a givenbiomolecule the actual molar binding capacitymight be different.
Coupling of a biotinylated protein to the covalently bound
streptadivin. After a pre-wash, simply add the biotinylated target
molecule in an appropriate buffer. In a short incubation step, the
biotinylated molecule will bind to the streptavidin molecule.
Immobilizer™ Plates Nickel-Chelate96 wells per plate. External dimensions 128 x 86 mm
l For detection and quantification of 6xhistidine-tagged
fusion proteinsl Covalently coupled Nickel-Chelate on the surfacel No blocking stepsl Low detection limitl Low backgroundl High signal to noise ratiol Stable at room temperature (for minimum 1 year)l Low reagent consumptionl Reaction site easily accessiblel White and black plates for luminescence or
fluorescence applications
Applicationsl Covalent Immobilisation
Coupling of a 6xHIS tagged protein/peptide to the Nunc
Immobilizer Nickel-Chelate Plate.
His = histidine
Immobilizer™ Plates Glutathione96 wells per plate. External dimensions 128 x 86 mm
l For detection and quantification of GST-tagged fusion
proteinsl Covalently coupled glutathione on the surfacel Ready to use - no activation steps or blocking stepsl Low detection limitl Low background noisel High signal to noise ratiosl Stable at room temperature (1 year)l Low reagent consumptionl Reaction site easily accessiblel White and black plates for luminescence and
fluorescence applications
Applications
Covalent Immobilisation
Coupling of a GST-tagged protein/peptide to the Nunc
ImmobilizerTM, Glutathione Plates.
GSH = Glutathione
GST = Glutathione-S-transferase
Applicationsl Covalent Immobilisation
InVitro PETG Roller BottlesFor industrial scale production of vaccines, monoclonal
antibodies or pharmaceuticals Moulded of durable PETG
Excellent substrate for adherent cells Quick-action
ergonomic closure reduces wrist stain and increases
productivity Wide range of sizes with surface areas from
1050 cm² to 4200 cm² Available in both Standard and the
patented Expanded Surface (XPS) Roller Bottle Easy-to-
read graduations for medium fills
l For industrial scale production of vaccines, monoclonal
antibodies or pharmaceuticals
l Moulded of durable PETG
l Excellent substrate for adherent cells
l Quick-action ergonomic closure reduces wrist stain and
increases productivity
l Wide range of sizes with surface areas from 1050 cm²
to 4200 cm²
l Available in both Standard and the patented Expanded
Surface (XPS) Roller Bottle
l Easy-to-read graduations for medium fills
Recently Introduced
l Holds 24 ounces
l Weighs 121 grams
l Sports a sleek, slender profile
l Allows for easy one handed operation
l Opens and closes with flip-top lid to eliminate 99% of leaks
l Let’s you enjoy the flavors of your beverage, not your bottle
l 0025901024 is Slate Blue
l 0025902024 is Sage Green
l Extremely durable
l Resistant to staining
l Resistant to retaining odors
l Recommended for “extreme” adventures
l Dishwasher Safe (top rack only)
l Withstands temperatures from -135ºC (-211ºF) to 135ºC (275ºF)
OTGIntroducing the New
You asked for it, we created it - the new
“On the Go”
bottle from NALGENE!
Made with
Polycarbonate
The Nalgene OTG
ELISA DEVELOPMENT KITSHuman IL-4 ELISA KitHuman IL-8 ELISA KitHuman INF-gamma ELISA KitRat IL-2 ELISA KitRat IL-4 ELISA KitRat IL-10 ELISA KitRat IL-12p40 ELISA KitHuman PDGF-BB ELISA KitHuman EGF ELISA KitHuman FGF-basic ELISA KitHuman VEGF ELISA KitMurine IL-2 ELISA KitRat IFN-gamma ELISA Development KitHuman IL-1-alpha ELISA KitHuman IL-2 ELISA KitHuman GRO-beta ELISA KitMurine MIP-1-alpha ELISA KitMurine JE (MCP-1) ELISA KitHuman IL-3 ELISA KitHuman sRANKL ELISA kitHuman CD40 Ligand ELISA KitHuman TWEAK ELISA KitHuman IL-5 ELISA KitHuman IL-6 ELISA KitHuman EOTAXIN-3 ELISA KitHuman IL-10 ELISA KitHuman IL-20 Elisa KitHuman CXCL16 ELISA KitMurine sRANKL ELISA KitHuman IL-17E ELISA KitHuman Resistin ELISA KitHuman EG-VEGF ELISA KitHuman TNF-alpha ELISA KitHuman IFN-gamma ELISA KitHuman GM-CSF ELISA KitHuman MCP-1 ELISA KitHuman RANTES ELISA Development KitHuman SCF ELISA KitHuman MIP-1 alpha ELISA Development KitHuman IP-10 ELISA KitHuman TPO ELISA KitMurine IL-3 ELISA KitMurine IL-4 ELISA KitMurine TNF-alpha ELISA KitMurine GM-CSF ELISA KitRat TNF alpha ELISA Development KitMurine SCF ELSIA KitMurine IL-1-alpha ELISA KitHuman IL-17A ELISA KitRat IL-1-beta ELISA Development KitHuman IL-1-beta ELISA KitHuman IL-12 ELISA KitMurine IL-12 ELISA KitMurine IFN-gamma ELISA KitMurine VEGF ELISA Kit
NEW
NEW
NEW
NEW
NEW
NEW
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM10
11
January 2006, Volume - 1
Nucleofection combines the advantages of various
transfection techniques:
1. Straight delivery into the nucleus ensures that expression
starts shortly after transfection and nucleofection of even
non-dividing cells is possible.
2. Upon cell division, DNA located in the cytoplasm can
be transferred into the nucleus, therefore increasing
transfection efficiency even further with time.
3. As DNA is also efficiently delivered into the cytoplasm it
guarantees successful siRNA experiments.
From nucleofection to protein expression in just a few hours
As the DNA can directly enter the cell nucleus, expression
of the transfected protein can be detected shortly after
nucleofection. In cell lines, transfection efficiencies of over
50% can be detected after just 2-4 hours. Even in primary
cells, including non-dividing cells, transgene expression
can be seen shortly after nucleofection. In unstimulated,
resting T cells for example, 15% of the cells express the
transfected gene after just one hour reaching up to 70%
after 16 hours.
Transient protein production offers.
Transient protein expression systems have gained
increasing relevance as they enable fast and flexible protein
production. Protein amounts of up to 10 mg can be
produced within a few days in contrast to production periods
up to several months using stably transfected cell clones.
Transient protein production offers:
l Fast production of small scale protein amounts
l Low costs for cell maintenance
In the past gene transfer into serum-free cultivated
mammalian cells suitable for protein production was
hampered by inefficient transfection methods. The
Nucleofector technology represents the ideal tool for gene
transfer even into hard-to-transfect cell lines like suspension
CHO cells. It has now been successfully tested and
validated for its use in transient protein production.
Benefit from transient protein production using
nucleofection:
l suitable for sCHO, sHEK-293, BHK-21, NS0 and many
other cellsl Applicable even under serum-free conditionsl High transfection efficienciesl Low DNA amounts
Numerous advantages of the Nucleofector technology
High transfection efficiencies: Transfection efficiencies of
over 50% can be obtained in most cell lines as well as in
primary cells. For some primary cells, such as human
dermal fibroblasts, efficiencies exceed 90%.
Short time until analysis: In most cell types, expression of
the transfected gene can be analyzed after just 2-4 hours,
rather than the standard 24 to 48 hours. This means that
Nucleofection - a revolution in transfection
DNA delivery straightinto the nucleus – idealfor transfection of non-dividing cellsNucleofector technology
achieves an efficient direct
transport of the DNA into the
cell nucleus. Consequently
transfection of cells using the
non-viral Nucleofector
technology is no longer
dependent on cell division i.e.
even non-dividing cells such
as resting blood cells or
neurons can be transfected
non-virally with high
efficiencies. The use of viral
systems for gene transfer into
non-dividing cells is therefore
no longer necessary.
From nucleofection to protein expression in just
a few hours.
As the DNA can directly enter the cell nucleus,
expression of the transfected protein can be detected
shortly after nucleofection. In cell lines, transfection
efficiencies of over 50% can be detected after just 2-4
hours. Even in primary cells, including non-dividing
cells, transgene expression can be seen shortly after
nucleofection. In unstimulated, resting T cells for
example, 15% of the cells express the transfected gene
after just one hour reaching up to 70% after 16 hours.
Mission #1: Transfect mouse T cellsIt took just a little bribery and the mouse unveiled the secret totransfecting its T cells. It’s not a trick, it’s nucleofection!Now available for the first time: non-viral transfection of mouse T cellsl up to 40% efficiencyl evaluated for most common
mouse strains: C57BL/6 andBALB/c
l maintenance of functionality
(e.g. stimulation)
Mission #2: Light up your cellsHappy cells shine bright when they are feeling vectorized withpmaxFP. It’s blindingly easy!NEW! pmaxFP fluorescent protein ex-pression vectorsl generation of fluorescent fusion
proteinsl very bright green, yellow or red
fluorescencel license free use for research
purposesl license free use for For-Profit
entities during the first sixmonths after purchase
l suitability for stable expression
Mission #3: Transfect humanmonocytesCatch of the day: human monocytes freshly prepared fornucleofection.Now available for the first time: non-viraltransfection of human monocytes!l Up to 60% efficiency.l High viability.l One technology for DNA and siRNA.l Complete solution - optimized culture
medium included
Mission #4: Protection againstmycoplasmaAntibiotics impressively demonstrate their determination to keepmycoplasma away from your cells.Use amaxa’s highly efficient antibi-otics to keep your cell culture myco-plasma-free!l New antibiotic formulations
against mycoplasmainfection.
l Protection or cure for primarycells or cell line cultures.
l No cytotoxic effects onprimary cells or cell lines.
l Compatible with nucleofection.
Mission #5: Transfect mammalianepithelial cellsWhatever epithelial cells you might have to take care of, theirwelfare couldn’t be commited to trustier hands than those of ourtwo experts.
New! amaxa’s Basic Epithelial CellNucleofector Kit for the transfection ofprimary mammalian epithelial cellsl transfection efficiencies of up to
80%l fast and easy optimization
l applicable for different mammalian
epithelial cells
Mission #6: Transfection of cancerand blood cell linesWith our easy strikers, cell lines formerly known as hard-to-transfect become a pure pleasure to work with. Make every shotcount!
There’s no better non-viraltransfection system for hard-to-transfect cell lines than amaxa’sNucleofector technologyl up to 98% transfection efficiency
e.g. for U-2 OS.
l Complete protocols including
cell culture details.
l up to 81% viability e.g. for A549.
Mission #7: Transfection ofmammalian neuronsIt’s the right combination of electropower and solution skills that keepsyour neurons excited for days.
NEW! amaxa offers first non-viraland highly efficient transfectionsolution for neurons, neural stemcells, astrocytes and oligodendro-cytesl Transfection efficiencies of up to 70%.
l Maintenance of functionality.
l Basic Neuron Kit for virtually all mammalian neurons.
Mission #8: Transfection of cell linesBrilliant transfections of hard-to-transfect cell lines from theproduction line with high efficiencies - almost visible to the nakedeye.
There´s no better non-viraltransfection system for hard-to-transfect cell lines thanamaxa´s Nucleofector
technology:l More than 300 cell lines successfully transfectedl Up to 98% transfection efficiencyl Complete protocols including cell culture details for more than
30 cell linesl Up to 81% viabilityl Optimization for all cell lines within 2 weeks
Mission #9: Transfect mammalianendothelial cellsNeither stifling heat nor spacious constriction can keep our cell-miner team from completing their transfection mission. They reallydig endothelial cells!
New! amaxa’s BasicEndothelial Cell NucleofectorKit for the transfection ofprimary mammalianendothelial cellsl Suitable for virtually any primary mammalian endothelial cell
type.l Fast and easy optimization.l Successfully tested for human, porcine and sheep endothelial
cells.
l Applicable for different mammalian tissue origins.
Mission #10: Transfection of cell lines
Our team’s markmanship is the reason why with nucleofectionthe DNA always enters the nucleus of the target cells.
amaxa’s Nucleofectortechnology - the best non-viral transfection systemfor difficult to transfectcell linesl Up to 84% transfection
efficiency, e.g. for RBL-1l Optimized protocols including cell culture detailsl Up to 94% viability, e.g. for COS-1l Optimization for all cell lines within 2 weeks
Mission #11: Transfection of humanmacrophagesNEW! The first efficient non-viraltransfection method for humanmacrophagesl Up to 60% transfection
efficiencyl maintenance of functionality
(e.g. activation)l Up to 90% viability after 24
hoursl for transfection of resting macrophages
Mission #12: Transfection of difficult-
to-transfect cell linesTransfecting difficult-to-transfect cell lines can be hugefun. It just takes know-how,practice and subtle fingers.Contact our experts to stay onthe ball.
Nucleofector technology- non-viral transfectionof difficult-to-transfect
cell lines!l Up to 85% transfection efficiency, e.g. for Saos-2l Optimized protocols including cell culture detailsl Up to 90% viability, e.g. for KG-1l Optimization for all cell lines within 2 weeks
Mission #13: Transfection of mousemacrophagessThe taming of the mousemacrophages was a major stepto getting them nucleofected.Our cell-charmers will be happyto tell you how it works!
New! The first efficientnon-viral transfectionmethod for mousemacrophagesl Up to 50% transfection efficiency.l Evaluated for macrophages from C57BL/6 and BALB/c strains.l For transfection of resting bone marrow derived macrophages.l Maintenance of functionality.
AMAXA’s Mission 2005
Nucleofection is superior to lipofection fortransient protein production.
In a comparative study the Nucleofector technology (amaxa)
and the lipofection reagent Lipofectamine™ 2000 (Invitrogen)
have been tested in regard to their suitability for transient
protein production. Nucleofection achieves 10-30 times higher
expression rates in CHO cells compared to cells transfected
with lipofection. A similar ratio has been determined for both
the specific and the volumetric productivities.
Combines cells of interest, expression
vector & the appropriate cell type
specific Nucleofector solution &
transfer to an amaxa certified cuvette.
Insert the cuvette into the Nucleofector and choose the cell type specific
Nucleofector program. Press the start button X after a few seconds
nucleofection is completed. Rinse the cuvette with culture medium and
transfer the cells into the culture flask. For 1x10 cells, ten cuvettes have
to be nucleofected.
Culture cells for several days
Harvest of
protein
Why Amaxas Nucleofector Technology is better than Conventional Electroporation!
experiments can be performed much more quickly, and
often completed within a single day.
Simple method: The Nucleofector technology is based
on electroporation, making it a quick method which can
be performed in less than an hour. It consists of the
Nucleofector device, which delivers the electrical
parameters, and Nucleofector Solutions, in which the cells
are contained while the electrical program is executed.
Consequently, the Nucleofector technology requires
minimal optimization (if any) by the user, making it the ideal
tool for gene transfer into primary cells, even by
inexperienced users.
The Industry standard forlabs and research settingFeatures:
Low in lint & low extrable wipers.Non-abresive wipers wont scratch ondelicate surface when used wet
Absorbent wipers hence easily wipe0-up liquid & dustMultifold despensing format enables one at a timedispensing which controls usage.Linguard Anti-Static Polyshield on each pop-up box helpsto further reduce the lint and static buildup.Suggested Area of use: Cleaning & wiping delicatesurfaces such as beakers, lenses, slides, opticle lenses,opticle instruments,surfaces glass layer etc.
For cleanroom applicationKimtech Pure CL 4 WipersSuitable for ISO Class 4 or higher
cleanroomFeatures:100% polypropylene costructionAcid, base & solvent resistantThermal bonding, no adhesive or binders
Delivered in anti-stat bagCan be autoclaved and ETO sterilized
Kimtech Pure CL5 WipersSuitable for ISO Class 5 or higher cleanroomFeaturesOur lowest extractable offeringMade from continuous filament rayon/polyster spun lace blend for a cloth like feel
Contain no binders or surfactantsHigh water absorbancy rate, plus excellent absorptionefficiency and capacityCan be autoclaved and ETO sterilizedSuggested areas to be used: Wiping Bio- reactors,wiping instruments, wiping inlet & outlet pipes
For Clean ManufacturingFeatures:
Made from Polypropylene constructionPerfect for use with oil and other solventsResistant to most of the chemicals andsolvents.Available in different dispensing formatslike: Roll, Flat sheet,Multi-fold, Quarter-fold
Suggested Area of use: Cleaning & wiping media andbio vessels, internal mechinary parts, tool & equipment,at packing points to provide finishing, mopping upspillages on floor.
For Versatile wipingFeatures:Designed to perform the wipingfunction which helps improveproductivity and performanceMade from advancedHYDROKNIT material which
deliver products that are absorbant, higher in strenth andhave more bulk.Bio degradable hence reduce disposable cost andenvironment friendlySuggested Area of Use: WYPALL wipers are an idealproduct to replace your manufactured rags (cloth towelsor rags).Available in different dispensing format like: Roll, Flatsheet, Multi fold, Quarter fold.
Kimberly- Clark ProfessionalPHARMACEUTICAL/BIOTECH
WORKPLACE PRODUCTS
SOLUTION TO ASSIST YOU TO
MAINTAIN BIOCLEANLINESS
RE-Newsletter-Volume1FF-October.pmd 1/7/2006, 2:50 PM11
12
January 2006, Volume - 1
PES SFCA CN NYL
PES (polyethersulfone) Is fast
and clean. PES is low protein
binding, so there is less change
of removing critical protein from your media. It is
hydrophilic, so no external wetting agents or
surfactants are needed, resulting in low
extractables. And it is fast, so you spend less time
waiting.
SFCA (surfactant-free cellulose
acetate) — It use wetting agents,
typically polyglycol ethers, to
overcome their natural hydrophobicity. These
substances have been shown to be toxic to
specific cell lines in vitro. The NALGENE
surfactant-free cellulose acetate (SFCA)
membrane is the ideal choice if you are growing
sensitive cells. SFCA is also low protein binding.
CN (cellulose nitrate) — CN filter
membranes have been around
since the 1940s. NALGENE CN
membranes are TRITON X® -free and have
excellent wetting properties. CN provides fast flow
rates for buffers and other aqueous solutions. CN
membranes are ideal for filtering and clarifying
solutions when protein binding is not a concern.
NYL (nylon) — NYL has very low
levels of extractables but binds
protein. It’s inherently hydrophilic
so it requires no wetting agents. And Nylon as
better alcohol resistance than any other MF75
Series membrane. Its toughness makes Nylon the
best membrane for special applications.
NALGENE MF75TM PESThe fastest tissue culture
membrane
NALGENE MF75TM SFCAThe cleaner CA membrane
for tissue culture application
NALGENE MF75TM CNThe proven membrane forgeneral-purpose filtration
NALGENE MF75TM NYLThe best membrane
for specializedapplications
NALENGE MF75TM filters are available in different sizes
Ask for it!
Why NALGENE Vacuum Disposable Filter Unitsare better than Competitor
Disposable
Unbeatable speed, low binding and easy to use!
Fast, Low binding and easy to use.
Supor is a registered trademark of Pall Corporation
Supor machV is an extremely clean, fast-flowing
asymmetric PES membrane that provides outstanding
throughput and low protein binding of cell culture media
and other solutions.
These great new filters feature:
l The fast flowing filter to save you time in the lab.
l Easy-to-use ergonomic shape
l Lot identification right on the product
l Guaranteed leak proof closure system
l NALGENE Certified performance
Available in 150mL, 250mL, 500mL, and 1L sizes.
Make Your Lab MORE Productive by Using...NALGENE® MF75™ Filters with New Pall Supor® machV PES Membrane
Filter most difficult to filter fluid such as SERUM very EASILY
Each Filter Unit has a side-arm with a cellulosic vent plug, a quick-
disconnect tubing adapter, and a leakproof threaded closure for
the receiver. Graduations are provided on both the upper and
lower receiver unit, allowing for easy sample assessment. The
Bottle Top Filters are designed to securely screw onto glass media
bottles. All MF75 Series Filters are gamma sterilized and
individually wrapped in easy-to-open bags.
NALGENE Filtration. So much variety, you are sure to be satisfied.Four different membranes and a wide variety of filter sizes, NALGENE offers the tastiest selection of filter units for any lab application.
Choose the best membrane for your application
Product Volume Nalgen M P S
Disposable 115ml 219.00 NA NA NA
150 ml 290.00 865.00 504.00 942.00
250 ml 402.00 900.00 504.00 -
500 ml 761.00 1500.00 790.00 1875.00
1000 ml 928.00 1591.00 790.00 1875.00
Bottle Top 150 ml 278.00 818.00 NA 550.00
250 ml NA 980.00 NA NA
500 ml 390.00 1360.00 NA 608.00
1000 ml 637.00 NA NA 1192.00
Available membrane-
SFCA PVDF PES CA
PES PES Nylon
CN
Nylon
…Because of its Maximum Membrane & Volume Range with LOWEST PRICES
N ALG EN E C orn ing M illiporeSupor m ach V PES m em brane h
PES m em brane h O ld G en M ill ipore h
C N m em brane h h h (m ixed ester)C A m em brane h
SFC A m em brane h
N YL m em brane h h
U nit S tability Excellent Poor PoorC ertif ication of Q uality in each case h h
C ertif ied for pyrogenic ity h h
C ertif ied for sterility h h
C ertif ied for retention h h
C ertif ied for bubble point h h
C ertif ied for f low h h
C ertif ied for m ateria ls h h
Post U se Integrity Testing av ailable h
Shelf li fe (Sterili ty) 5 years 2 years 3 yearsG uaranteed Leakproof C losure h
50m m m em brane diam eter h h
75m m m em brane diam eter h h (70m m ) h (70m m )90m m m em brane diam eter h 1000m l only N oC olour coded collar h h
Pore size identif ied h (on upper) h (on upper) h (on collar)M em brane identif ied h (on upper) h (on upper) h (on collar)C atalog num ber identif ied h (on upper) h (on bag) h (on bag)Lot num ber identif ied h (on upper) h (on bag) h (on bag)C onstruction W elded Friction f it W eldedP ictogram instructions on each bag h
Vacuum tubing adapter A ttached D etached A ttached
New
NALGENE
Metabolic Cage For MiceSpecifically designed for urine and faeces collection from a single mouse
Allow efficient seperation and collection of urine and faeces
New design reduces evaporation and sample loss
l 75% more urine collection and current
metabolic cages means you get adequate
sample volume from even one mouse
l smaller chamber volume and collection tube
design minimizes evaporation
l Proven funnel & cone design with steeper angle
seperates urine & faces into seperate collection
tubes
l Non wetting funnel & cone material ensures
immediate complete seperation of urine &
faces.
l Self-standing for single cage use (Single cage
stand not required)
l Available separately, With support plate will fit
in new Nalgene Metabolic Cage Racks.
NEW
NEW
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