Introduction to training
Agenda• Product overview
• What’s new from ProStar 325
• Hardware overview
• Initialization routine
• Software CD overview
• Detector installation & PolyVerfiy
• PolyDiagnostics
Product Overview
Based on the ProStar 325 with many shared parts
Two models available:
• Single 1024 array • Dual 1024 array
Control by both Star with PolyView 2000 and Galaxie(PolyView is now included with Star driver)
Product Overview - workstation control
Galaxie• Four 335 DADs per acquisition server
• Driver included in Varian LC control drivers
Star• two 335 DADs per Star Workstation
• Driver included in LC control option
• Installation of driver automatically installs PolyView 2000
Ethernet communications best with a switch (not a hub)
Product Overview
• Same cabinet• Same UV & visible lamps• Same flow cells• Same rear panel, connectors, interface boards & cables• Same communications protocol• Same power supply• Same Main pwb (difference in firmware)
ProStar 335 and 325 similarities
What’s New
Same look as ProStar 325 except no PPC capabilities
What’s New
Dual Array• 1024 photodiode sample and reference array
• Extended range spectra
• Used for Analytical to Prep Applications
Single Array• 1024 photodiode sample array
• 2 photodiode references (250 and 500 nm)
• No extended range
• Uses 9x0 and 4x0 flow cells only
Different optics module
Hardware Overview – optics module
Product Overview – ray trace diagram
Hardware Overview - Polychromator
Available slit widths
1nm2nm4nm8nm
16nm
Hardware Overview – dual path flowcell
Illustration is of a 9x1 flowcell
sample
reference
Hardware Overview – optics module
Wavelength calibration data must be entered into the EEPROM using Diagnostics software.
Electronics – wavelength calibration factors
Use the floppy disk to update the EEPROM
automatically
This label will be attached to the top of each optics module
Hardware Overview – Electronics
Only difference between 335 & 325 is firmware
Hardware Overview - Electronics
Add new MAC address label when swapping pwb’s
New MAC address label comes with each new PWB
Hardware Overview – initialization routine
• Peak search for 656.1nm deuterium emission line
• Determines dark current table (0%T)
• Gain (Lamp) calibration is now user driven. Stored in non-volatile memory.
Initialization
Status LED’sinitialized
not initialized
Hardware fault
Software CD overview
Software CD ships with each ProStar 335 detector.
Part number: 85 102114 00
Auto-loading CD which contains:• Galaxie drivers and manuals• Star driver and manuals• PolyVerfiy software• PolyDiagnostics
Software CD overview
Customer software CD 85-1xxxxx-00 will contain
Software CD overview – installation PolyVerify
Use PolyVerify to check the performance of the ProStar 335
After installing, a desktop icon will be created called
ProStar
Software CD overview - installation PolyVerify
Only two test are required at installation;
Noise/Drift Wavelength
accuracy
IQ/OQ is also availablepart number: 03-914756-00;4
Software CD overview – installation PolyVerify
Print customer certificate
The ProStar 335 must be initialized and have both lamps on for at least one hour prior to running any tests.
Noise test : pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.
Wavelength accuracy: same conditions as the Noise test.
Software CD overview - PolyDiagnostics
Browse the CD using Windows Explorer.
Diagnostics is not self loading and not intended for
customer use.
Installing PolyDiagnostics
Software CD overview - PolyDiagnostics
To run PolyDiagnostics you will need to copy these three files to the ProStar directory where PolyVerify is installed. From there double click the “PolyDiagnostics.exe” file using Windows Explorer.
Software CD overview - PolyDiagnostics
Similar appearance to
325 Diagnostics
Software CD - PolyDiagnostics
Service folder also contains the firmware updater program
Use the Diagnostics program to update the EEPROM and perform various calibration routines i.e. wavelength & lamp calibrations
Software CD - PolyDiagnostics
The EEPROM contents will need updating whenever;
• the Main PWB is replaced• the optics module is replaced• to update installation & maintenance dates
Software CD - PolyDiagnostics
EEPROM contents;
• Detector serial number• MAC address• Wavelength calibration factors, slope and offset• IP address• Gateway address• Subnet mask• Lamp timer (on/off date and times)• Detector installation date• Last maintenance date• Error log• Hg wavelength calibration date
Software CD - PolyDiagnostics
When to calibrate
Performing a Lamp Calibration will help reduce noise and improve linearity.
• Deuteriunm lamp is changed• Visible lamp is changed• Every 1000 lamp source hours• Increasing signal noise• When changing flowcell to one of a different pathlength