Transcript
Page 1: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Introduction to Microbiology

Page 2: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Where do Microorganisms come

from?EVERYWHERE!!!!They are all around us, in this lab

we will examine various microorganisms in the environment around us.

Page 3: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part I-Test Tube

Obtain a test tubeFill 1/4 full with soilAdd water to test tube until it is

about 1/3 full. Cap test tube and shake.

Place your names on the label.

Page 4: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part I- Petri Dish

Obtain a petri dish and place your names on the label.

Pour a layer of hot, melted agar into the petri dish. Fill your petri dish about 1/4th full.

Recover your petri dish.Allow the agar to harden for about 5

minutes.

Page 5: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Locate a sterile transfer loop.Place the plate in front of you.Dip the loop end of the transfer loop into

the soil water and transfer the soil water to the agar plate.

Gently rub the surface of the agar with loop. Don’t push too hard.

Transfer two more loopsful of soil water in the same manner.

Observe your plate and make a drawing of what you see.

Page 6: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part II- Fungi

Obtain another petri plate, and put a label with your names.

Add a thin layer of tomato soup to the bottom of the plate.

Grind up a small sample of dirt in your fingers.Sprinkle a few specks of dirt on the soup and

cover the plate.Observe your plate and make a drawing of

what you see.

Page 7: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part III- Yeast test tube

Obtain a culture tube, and label with your names.

Add yeast growth medium to the tube until it is 2/3 full.

Locate the packet of dry yeast.Add one of the small particles of yeast to the

tube.Shake the tube with the top on until you can’t

see the particles of yeast any longer.

Page 8: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part III- Yeast Slide

Obtain a sterile loop.Dip the end of the loop into the yeast liquid in the

tube.Transfer a small amount of liquid onto a clean glass

slide.Repeat this 4 more times so you have a drop of the

yeast growth medium on the slide.Add a cover slip and observe the yeast under high

power on a microscope.Count the number of cells you see in the field.

Page 9: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part IV- Algae and Protozoa

Locate the large plastic bottle filled with pond water.

Obtain a clear glass slide and a cover slip. Place a drop of pond water on the slide

using a pipette.Place cover slip on slide.Observe under low and medium power.Make drawings.

Page 10: Introduction to Microbiology. Where do Microorganisms come from? EVERYWHERE!!!! They are all around us, in this lab we will examine various microorganisms

Part V- Soil CultureObtain your original tube containing the soil

and water after about 4 days. Obtain a clear glass slide, a cover slip, and the

bottle of methylene blue stain. Transfer a drop of soil water to the slide.Spread the water around on the slide.After the water has dried, add a drop of the

methylene blue stain, and let it dry for 1 minute on the slide.

Carefully blot the slide dry with a paper towel.Observe the slide under high and low power.Make drawings of what you see.


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