If = Hypothesis, Then = PredictionPrediction= What you think the outcome of a specific experiment will be
Hypothesis: Why
If /then statement is NOT A HYPOTHESISYou will be marked wrong.
You Should Know
• Scientific Method• Science Terms– Control– Controlled Variable,– Indpendent/dependent variables– Sample size– Significance– Etc….
• Tools we have learned in lab. From onions- transformations. Big picture.
• Hint: We are testing Scientific reasoning and problem soloving.
Transformations Terms
– Biotechnology: using technology to manipulate organisms
– Genetic Engineering: Manipulating genes in organisms
– DNA Cloning: putting a segment of DNA in an organism and having that organism make tons of copies of the gene, gene product or DNA segment.
– Transformation: Introducing DNA from one organism (usually bacteria) into another organism.
Very Expensive
2 stops
Will be Graded on Technique
Be patient
Restriction Enzymes Leave Sticky Ends
Transformation
http://www.youtube.com/watch?v=aA5fyWJh5S0
How do we know it worked?
• Must determine if Gene was Inserted Successfully into Plasmid– Marker Genes!– GFP– Disrupt function and it disappears
• Antibiotic: tells if plasmid made it in to cell• Color/Glowing: tells if gene made it into
plasmid
Problem
Plates
• Groups of 3 or 4. • Half Plates: step 7 page 104 use half volumes– 50 instead of 100, 25 instead of 50
• Check Plates 48 hours later mark on plate cover what you found. – Leave in incubator for me to grade.