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GlyphosateinMMR:DoesThisExplaintheAu7smLink?

StephanieSeneffCSAIL,MIT

Tuesday,Sept.19,2017

“Itisdangeroustoberightinma2ersonwhichtheestablishedauthori6esarewrong.”

–Voltaire

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Outline

•  Whatisglyphosate?•  Howglyphosateaffectsthegutbarrier•  Glyphosateinvaccines– GlutamateandglyphosatesynergyinMMR

•  Autoimmunediseasethroughmolecularmimicry•  Summary

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RoundupandGMOCrops

GMORoundup-Readycorn,soy,canola,sugarbeetscoOon,tobaccoandalfalfa

Whatisglyphosate?

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RoundupasaDesiccant/RipenerjustbeforeHarvest

Wheat,Oats,Barley,Rye,Sugarcane,Beans,LenSls,Peas,Flax,Sunflowers,Pulses,ChickPeas

*Figure15,Seneffetal.,AgriculturalSciences,2015,6,42-70

Au7smPrevalence:6yearolds*

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IsGlyphosateNontoxic?•  Monsantohasarguedthatglyphosateisharmlesstohumansbecausewedon’thavetheshikimatepathwaywhichitdisruptsinplants

•  However,ourgutbacteriaDOhavethispathway–  TheyuseittosupplyuswithessenSalaromaScaminoacids(precursorstoneurotransmiOers,thyroidhormoneandBvitamins)

•  OtheringredientsinRoundupgreatlyincreaseglyphosate’stoxiceffects

•  InsidiouseffectsofglyphosateaccumulateoverSme–  Moststudiesaretooshorttodetectdamage

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GlyphosateandtheGut

•  GlyphosatehasbeenfoundasacontaminantindigesSveenzymestrypsin,pepsinandlipase*

•  Trypsinimpairmentpreventsproteinslikegluteninwheatfrombeingdigested

•  Undigestedproteinsinducereleaseofzonulinwhichopensupgutbarrier**

•  ZonulinlingersbecausetrypsinisdefecSve

*ASamselandSSeneff.JBiolPhysChem2017;17:8-32**JJGildeaetal.JClinNutrDiet.2017,3:1.

AScenario

ZonulinTrypsindefecSve

Zonulinreleased

LeakybarrierGliadin

Leakybrainbarrier

Glyphosateinwheat

Autoimmunedisease

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Whatifglyphosatecouldinsertitselfintoproteinsynthesisbymistake???

GGC

mRNA

glyphosate

Alanine ProlineGlyphosate

Anyproteinswithconservedglycineresiduesarelikelytobeaffectedinamajorway

......

Codeforglycine

Whatifglyphosatecouldinsertitselfintoproteinsynthesisbymistake???

GGC

mRNA

glyphosate


Anyproteinswithconservedglycineresiduesarelikelytobeaffectedinamajorway

......

Codeforglycine

Trypsincontainsseveralglycine-richregionsthatareessenSalforitsfuncSonasadigesSveenzyme

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H

GlycineGlyphosate

Glyphosateisanon-codingaminoacidanalogueofglycine

ExtraPieceS7cksOutatAc7veSite


glycine

AcSveSite

ExtraStuff

PepSdechain

SubstratenolongerfitsinacSvesite

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ExtraPieceS7cksOutatAc7veSite


glycine

AcSveSite

ExtraStuff

PepSdechain

SubstratenolongerfitsinacSvesite

Thisexplainshowglyphosatedisrupts

EPSPSintheshikimatepathway:MulSplebacteriahavedevelopedresistancebyreplacingacSvesiteglycinewithalanineandthisisthe

basisforGMORoundupReadycrops*

*TFunkeetal.,MolecularbasisfortheherbicideresistanceofRoundupReadycrops.PNAS2006;103(35):13010-13015.

Inhibi7onofEPSPSbyglyphosate:ResistantEcolimutant*

*Figure3,SEschenburgetal.Planta2002;216:129-135.

Wildtypewithglycine96

MutantwithalaninesubsStutedforglycine96

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GlyphosateinVaccines?•  ForMMR,fluvaccine,andrabiesvaccine,livevirusisgrownongela'nderivedfromligamentsofpigsandfetalbovineserum– CowsandpigsarefedGMORoundup-Readycornandsoyfeed

•  GelaSnisderivedfromcollagenwhichishighlyenrichedinglycineandalsocontainsglutamate– ThesetwoneurotransmiOersexcitetheNMDAreceptorsinthebrain

•  GlyphosatesSmulaSonofNMDAreceptorscouldcauseneuronalburnout

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GlyphosateExcitesNMDAReceptorsinHippocampus*,**

*DCaOanietal.Toxicology2014;320:34-45.**DCaOanietal.Toxicology2017[Epubaheadofprint]

Excessglutamateinsynapseleadstoneuronalburnout


*DCaOanietal.Toxicology2014;320:34-45.**DCaOanietal.Toxicology2017[Epubaheadofprint]


Ratsexposedtoglyphosateperinatallyexhibiteddepressivebehaviors

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*ZZhengetal.PLOSONE2016;11(7):e0158688.


Glutamateiselevatedinthebloodin

associaSonwithauSsm,andbloodglutamatelevelscorrelatewithbrainglutamatelevels.*

Au7sm,Glyphosate,VaccineReac7ons*

*DatareadilyavailablefromtheCDC,FDA(VAERS)andUSDA

GlyphosateapplicaSontocornandsoy,US

#AdverseReacSonsinVAERS

ChildrenwithAuSsm,US

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*ASamselandSSeneff,JournalofBiologicalPhysicsandChemistry2017;17:8-32.


…Mostdisturbingisthepresenceofglyphosateinmanypopularvaccinesincludingthe

measles,mumpsandrubella(MMR)vaccine,whichwehaveverifiedhereforthefirstSme.

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GlyphosateContamina7oninVaccines(PartsPerBillion)*

Merck ZOSTAVAX 0.62ShinglesMerck MMR-II 3.74Measles,Mumps andRubella

Merck VARIVAX 0.56 Varicella,ChickenPoxMERCK PNEUMOVAX ND Pneumococcal18MERCK PROQUAD 0.66Measles,Mumps, Rubella,Varicella

GSK ENERGIX-B 0.34HeptaSSsB


SymptomsofAdverseReac7onstoMMRbeforeandaVer2002*

DataanalyzedfromtheVAERSdatabase*ASamselandSSeneff.JBiolPhysChem2017;17:8-32

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SymptomsofAdverseReac7onstoMMRbeforeandaVer2002*

*DataanalyzedfromtheVAERSdatabase

TheseareallcharacterisScsymptomsofallergiestoMSG

seizures

hives

swellingswelling

Jointpain

FacialswellinganaphylacScshock

shortnessofbreath

eczema

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“NobelLaureateCharlesRichetdemonstratedoverahundredyearsagothatinjecSngaproteinintoanimalsorhumanscausesimmunesystemsensiSzaSontothatprotein.SubsequentexposuretotheproteincanresultinallergicreacSonsoranaphylaxis.Thisfacthassincebeendemonstratedoverandoveragaininhumansandanimalmodels.”

LargeProteinsinVaccines:Allergenic*

“VaccinesclogourlymphaScsystemandlymphnodeswithlargeproteinmoleculeswhichhavenotbeenadequatelybrokendownbyourdigesSveprocesses,sincevaccinesbypassdiges'onwithinjecSons.Thisiswhyvaccinesarelinkedtoallergies,becausetheycontainlargeproteinswhichascirculaSngimmunecomplexes(CICs)or'klinkers’causeourbodytobecomeallergic.”

*DaveMihalovic,ND,hOp://whale.to/v/vaccines_cause_allergies.html

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ThePurdueVaccina7onStudiesandAutoan7bodies*

“ThePurduestudies… foundthatvaccinateddogsweredevelopingautoanSbodiestotheirowncollagen”

*hOp://www.dogsnaturallymagazine.com/purdue-vaccinaSon-studies/

AScenario

2.Livemeaslesvirusinfectsgut

3.MeasleshaemaggluSninundigested

4.Inducesleakygut

6.Zonulininducesleakybrainbarrier

6.Measlesvirusinfectsbrain

7.Brain’simmunesystemproducesanSbodies

8.AutoanSbodiesaOackmyelinsheath

1.ChildisvaccinatedwithMMR

5.Measlesvirus

5.Zonulin

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MeaslesVirusandHaemagglu7nin*•  ThemeaslesvirussynthesizestheproteinhaemaggluSnin

–  AnSbodiestohaemaggluSninareessenSalfollowingMMRvaccinaSontoinduceimmunity

•  Measlesvirusinfectsbrainduetoleakybarrier•  HaemaggluSnbearsasequenceresemblancetomyelinbasic

protein(MBP)àpotenSalforautoimmunereacSon•  MBPisessenSalfortheformaSonofthemyelinsheath

surroundingnervefibers•  AutoanSbodiestoMBPalongwithexcessivelevelsof

anSbodiestomeasleshaemaggluSninarelinkedtoauSsm**

*Oldstone,MBA,Ed.Molecularmimicry:InfecSoninducingautoimmunedisease.SpringerBerlinHeidelberg;January9,2006.**VKSinghetal.,JBiomedSci2002;9(4):359-64.

Au7smandMeaslesHaemagglu7nin*

•  125auSsScchildrenand92controlchildren•  60%ofthechildrenwithauSsmhadhighlevelsofanSbodiestomeasleshaemaggluSninspecifictotheMMRvaccine–  90%ofthesehadautoanSbodiestomyelinbasicprotein(MBP)

•  0%ofthecontrolchildrenhadhighanSbodySterstoeitherhaemaggluSninorMBP

•  TherewerenoelevaSonsinanSbodiesdetectedagainstanyproteinsinthemumpsorrubellaviruses

*VKSinghetal.,JBiomedSci2002;9(4):359-64.

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Summary•  Glyphosate’sinsidiouscumulaSvetoxicitymaybeduetoits

abilitytogetintoproteinsbymistakeinplaceofglycine•  GlyphosatehasbeendetectedinmulSplevaccines,with

highestconcentraSonsinMMR–  ManyvaccinesincludegelaSnandfetalbovineserumasingredients,

bothofwhicharecontaminatedwithglyphosate

•  Glyphosateandglutamate(fromthevaccine)canexciteNMDAreceptors,andcauseneuronalburnout

•  GlyphosatecontaminaSonleadstoresistancetobreakdownandautoimmunediseasethroughmolecularmimicry–  AcausallinkbetweenMMRandauSsmcanbeexplainedthrough

autoimmuneaOackonmyelinbasicproteinviamolecularmimicrywithmeasleshaemaggluSnin

•  MulSplelargeproteinsfoundinvaccinesareallergenicandtheymaybeacausalfactorinfoodallergiesandanaphylaxis