From the Box to Packing in 30 minutes
Pack-in-a-Box components
• Remove components and assemble bracket as shown
• Follow the instructions to pack your first column and learn how to use the system
Additional Equipment & Chemicals
• ¼” wrench• 5/8” wrench• 7/16” wrench• Phillips head screwdriver• 30ml beaker• Waste container• Methanol• Chloroform• Spatula• Balance• Computer• Ultrasonic bath
• Pre assembled for supplied SSI column hardware.
Column Packer Assembly
Mounting Packing Stand to Pump
• Screw stand to side of pump using the two #6 screws provided.
Plumbing the Pump
Priming pumpsUse MeOH for this
column• Open prime purge
valve press prime button
• With prime purge valve open use the supplied syringe to draw solvent through the pump
• Prime pump
• When pump is primed close the prime purge valve
Packing Ring Seal Installation
• Place ring in adapter as shown
• Make sure ring is flat in bottom of adapter
Packer Mounted to Stand
• Mount packer to stand and tighten wing nuts
• No wrenches are required to seal reservoir
• Hand tighten end nuts
Column Assembly
• Assemble lower fitting of column and add frit to top end fitting and set aside to put on column after it is packed.
System Assembled Correctly
• The use of the Quick-Set software is not required but gives the most flexibility and will be used for this example
Installing Quick Set
• Insert Disk• Run the Quick-Set “setup.exe”
program file & follow prompts.• Due to the many versions of
Windows in use, your PC may have newer files than the ones being loaded. You will be prompted for a course of action, when in doubt, “keep” your existing files or “ignore” other messages.
• Connect RS 232 Cable provided to the computer and pump
• Turn on pump
Click QuickSet
• Go to Start/Programs
• Click on Quick-Set
• The screen below appears with the information on the program
Automatic Set Up
Initial screen is for locating the SSI / LA pump
If communication does not initialize or if the pump is not on this list: Click on the “Poll Com Port For Device” button.
This will scan 9 possible PC COM ports for the attached device.
When the device is found, click on the” Continue” button.
Getting To CP Screen
Setting Flow and Pressure
• Set to pressure limited ramp and click ‘send’
• Set upper pressure to 9999 psi; click ‘send’
• Set lower pressure to 0 psi; click ‘send’
• Set ramp speed to 100; click ‘send’
• Set max flow and pressure from table
Adding Steps
Packing program to load
Pressure Flow Rate Time
9000 24 1
9000 23 5
9000 22 30
Send to pump
Saving Your Packing Program
• Click on “Save Settings”
• Enter file name
• Save
Mounting Column
• Make sure packing ring seal is in adapter
• Hand tighten column in adapter
• Place plug in bottom of column
• Remove plug just prior to starting pump
Tighten Column on Packer
• Use a ¼” wrench and 5/8” or adjustable wrench to snug column to packer
• Do not over tighten ¼ turn past snug is fine
• Fill column with 3 mL MeOH
Setting up Graphing and Data Collection
Saving Packing Data
• Select Log data
• Give file a name
Making the Slurry
• Weigh 3 gm of silica into 30 mL beaker
• Add 20 ml of 50:50 Chloroform:Methanol (Straight MeOH can be used but is not as efficient)
• Use a spatula to break up packing• Place in ultrasonic bath until
uniformly dispersed• Pour in packing reservoir as soon as
possible and pack column
Adding Slurry
• Use 20 mL of silica slurry and pour carefully into reservoir
• Fill reservoir with washings of beaker and close
Is It Closed?
• Make sure the reservoir is full with slurry
• Remove silica from threads
• Hand tighten top nut
Make Sure You Are Ready
• Is the program loaded?• Is the pump primed?• Is there a waste collection
vessel below the column?• Did you tighten the fittings?• Is there slurry in the reservoir
and is the reservoir closed?• Are you set to graph the process
and save your data?• If so, proceed
Packing The Column
• To run program, click on “Pump;” or if program is loaded click on “Start”
Initial Packing Profile
Running a Column Packing Profile
Removing the Column
• Stop the pump and let the pressure drop to 0 psi
• Carefully unscrew the column, and using a flat spatula or razor blade, remove excess silica
• Remove silica from threads if present
• Place end fitting and frit on column and seal
Removing Excess Silica
• Work quickly with a spatula edge or razor blade
Getting Ready to Pack Again
• Pump remaining silica from precolumn for reuse or disposal
• Empty reservoir
• Install next column and repeat steps