Extraction of Peptides and
Amino Acids
Wim MulderSustoil, Foggia 24 April 2009
Composition oil cakes
Table 1. Composition of rapeseed, sunflower and olive pulp
Rapeseed Sunflower Olive pulp Water 5.0 6.7 50.0 Oil 41.6 53.3 22.0 Protein 26.2 22.9 1.6 Carbohydrates + fibres 23.4 14.0 24.9 Ash 3.8 3.1 1.5
Extraction of peptides and amino acids relevant for rapeseed and sunflower cake
Amino Acids
Nutritional value Taste Physiological/chemical characteristics
Production Chemical Enzymatic Fermentation Extraction
Human food
Animal feed
Oil cakes Hydrolysis
Protein hydrolysis
Complete hydrolysis 6 n HCl, 110°C, 24 hours 5 n NaOH, 100°C, 18 hours Salts incorporated Destruction amino acids
Enzymatic hydrolysis Peptides Immobilised enzymes
Extraction before oil recovery
rapeseed or sunflower seed
rest seedspeptides and amino acids
aqueous hydrolysis
oil
pressing
fractionated amino acids
Extraction after oil recovery
rapeseed or sunflower press cake
soluble proteinspeptides and amino
acids
acidic/alkaline extractionenzymatic hydrolysis
peptides and amino acids
aqueous hydrolysisfractionated amino acids
fractionated amino acids
Simultaneous extractionground oilseed
alkaline extraction centrifugation
solids: protein concentrateliquid
oil emulsion
acidic extraction centrifugation
3-phase centrifugation
liquid solids
3-phase centrifugation
oil
oil emulsion liquid
oil protein isolate
precipitation
ground oilseed
alkaline extraction centrifugation
solids: protein concentrateliquid
oil emulsion
acidic extraction centrifugation
3-phase centrifugation
liquid solids
3-phase centrifugation
oil
oil emulsion liquid
oil protein isolate
precipitation
Chemicals from amino acids
Biobased route for butanediol
Biomass
Proteins
Sugars
Amino acids
Other/rest amino acids
for food, feed or
chemicals
1,4-buanediol
Succinic acid
Conclusions Biorefinery on protein-rich cakes
Extraction processes Chemical Enzymatic
Use of proteins/peptides requires mild oil extraction
Production of biobased chemicals is important
Research necessary Obtaining amino acids Fractionation of amino acids
Remarks/questions
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