DNA FINGERPRINTING
The story…The story…
1) Provide a timeline1) Provide a timeline
2) Draw a family tree2) Draw a family tree
3) How could you explain Tom’s 3) How could you explain Tom’s resemblance to TJ?resemblance to TJ?
4) Why is the Y chromosome a good 4) Why is the Y chromosome a good marker?marker?
5) What is a haplotype?5) What is a haplotype?
6)How can the descendants be used to 6)How can the descendants be used to indicate paternity?indicate paternity?
DNA
• Double helix
• A = T C = G
Restriction Enzymes
• Bacterial origin• Cuts DNA sequences into shorter fragments• Site specific
Ex: EcoR1 cuts at GAATTC sequence
different DNA sequences
Living organisms that have different characteristics
The more different the organisms
the more different are the DNA sequences
Restriction EnzymesRestriction Enzymes
www.uic.edu/classes/phar/phar331/lecture6/
DNA FINGERPRINTINGDNA FINGERPRINTING
http://www.biotech.iastate.edu/biotech_info_series/bio6.html#anchor861530#anchor861530
Professor Sir Alec Jeffreys
DNA Fingerprinting-DNA Fingerprinting-
Who’s the father?
Are you sure?
Use Multiple Probes
Eric S. Lander
An Meeker-O'Connell