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INTROwithin spontafollowestudiesDiffusiadditiomembmicroskurtosdevelogrey aMETHgrounddemyeMR syms, slgradie(MK), NatickregionventrameasuRESUhistoloconfirmwith pthrougMK anconsistreatmcompareduceearly sthese histoloK//, K┴reflectcomplemice wabnorm
ard S Hui1, Josephadiology, Medical
ODUCTION: Thweeks after yo
aneous remyelined by demyelinas have demonstonal Kurtosis Im
onal information branes, organellestructural compleis metrics provid
opment15 and in dand white matter HODS: A total ofd mouse chow elination. The conystem. A respiratlice thickness=1
ent direction (0.5axial (K//) and ra
k, MA) called Diffs of interest (RO
al (vHP)) were murements betweeLTS & DISCUSS
ogical stain (Solom demyelination.previous reports, ghout the entire Cnd K┴ throughoutstent with the d
ment). The axial dared to that of ed only in the bCstages of patholoaxial diffusion ch
ogical correlation┴ showed signifis cortical demyeexity not only in with demyelinatimalities in grey m
Diffusionah A Helpern, 1, DavUniversity of Sout
United St
he cuprizone mooung adult mice ation occurs2,3.
ation associated trated the patho
maging (DKI) is a beyond that pro
es) and water coexity in the greydes better differedifferent diseaseduring the demyf 22 (8–10 weekscontaining cuprntrol (NC) group tion-gated 4-shot mm, data matr, 1, 1.5, 2 and 2adial (K┴) kurtosifusional KurtosisOIs) at the level manually drawnen CPZ and NC mSION: Illustratedochrome) for NC. Fig.2 shows theCPZ mice show
CC. Also, the nont the entire CC indegree of demydiffusivity λ// wasNC, and the K//
CC. Since axonaogy (before 4 wehanges (at 10 wen. A striking resuicant changes (elination and reawhite matter, buon induced by c
matter.
Fig.2. cuprizoregionand c(dorsaare sta
al Kurtosis Detvid Guilfoyle2, Scoth Carolina (MUSCtates, 3Dementia R
ouse model is a are fed with th
In this model, dwith a microglialogy of the CC idiffusion MRI tec
ovided by DTI12-1
ompartments (exy matter in additntiation of brain
es processes6,18. yelination processs old) C57BL/6 mizone (0.2%), (B(n=10) was maint SE-EPI sequenrix=128×128, im.5 ms/μm2). Fracs were derived f
s Estimator (DKEof corpus callos using ImageJ mice. P < 0.05 w in Fig.1 are rep
C and CPZ moue ROI measuremwed significantlyn-Gaussian diffusn the CPZ mice. yelination seen s increased in th/ showed a trenl damage in this eeks of cuprizoneeks of cuprizonult was that onlydecrease) in the
active glial cells ut also in grey macuprizone, demo
ROI measuremeone treated (CPZ)s: corpus callosum
caudal (pCC)), col (dHP) and ventra
andard deviations.
tects Cortical ott Gerum2, CaixiaC), Charleston, SC
Research, Nathan K
well characterizhe copper chelademylination is pal response3,4. Rein cuprizone mochnique that exte4. Since non-Ga
xtracellular and intion to white matissue type, and The main goal o
s in the cuprizonmice were used Bis(cyclohexanonntained on a nornce was used fo
mage resolution=ctional anisotropfrom the DKI dat
E))20. All parametum (rostral (aCC((http://rsb.info.n
was considered apresentative b0 im
se brain. Solochment of different y reduced FA ansion metrics showThese diffusion at this phase (
he bCC, but decnd for decrease
model is variablne treatment)7, the treatment) can
y the non-Gausse cortex of the accumulation5, catter. In summaronstrating the sig
ents of diffusion ) and control (NC)m (rostral (aCC), mortex (CT) and al (vHP)); * p < 0.0
Demyelinatioa Hu2, John LaFranC, United States, 2MKline Institute, Ora
zed animal modetor cuprizone (b
predominantly foecently, cortical
ouse model6-11. Hends DTI and quaussian diffusionntracellular), the
atter structures. are sensitive to
of this study wase mouse model.in this study. In ne) oxaldihydrazmal diet for 10 wr DKI acquisition234×234 μm2, 4
py (FA), mean (Mta set14 using antric maps were mC), middle (bCC)nih.gov/ij/). Two-s statistically sig
mages, MK mapshrome was perfobrain regions. Cnd increased MDwed significantlychanges are qua(10 weeks of creased in the pC, but it was sigle and more promhe exact interpre
n only be done wian diffusion meCPZ mice, wh
confirming that ry we observed, gnificant advant
metrics for ) mice. Brain
middle (bCC), hippocampus
05. Error bars
on in the Cuprncois3, Xingju NieMedical Physics, Nangeburg, New Yo
el of demyelinatbis-cyclohexanonound in the corp
demyelination hHowever, no coruantifies the non-n is believed to ae measures of D
Indeed, severachanges in brain
s to quantitatively the cuprizone (Czone, Sigma-Ald
weeks. All in vivo n. The sequence4 averages, 30
MD), axial (λ//) ann in-house softwamasked (MD> 1.5), and caudal (pC-tailed t-test wa
gnificant. s and the ormed to onsistent D and λ┴ y reduced alitatively cuprizone CC when gnificantly minent in etation of
with future etrics MK, ich likely kurtosis metricsfor the first time
tage of microstru
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DKI can be consial animal studiesn microstructuray characterize th
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he diffusional kur
up (n=12) mice wiod of 10 weeksts were performe
ere: TR/TE=3000ons19 and five bfusivity, as well ad in Matlab (The duce partial volu), hippocampus
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aria F Falangola1,2
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supported by NI34-01.
histological staated group.
ult n, e
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8) 5) n n
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