College of Agriculture and Life Sciences
Department of Plant Breeding
Developing Breeding Lines with Anthracnose Stalk Rot Resistance
from Exotic Maize Germplasm
Margaret Smith
College of Agriculture and Life Sciences
Department of Plant Breeding
Anthracnose Stalk Rot
• Colletotrichum graminicola (Ces.) G.W. Wils.
• Increased lodging, reduced yield
• Best control through resistant varieties
College of Agriculture and Life Sciences
Department of Plant Breeding
Overall Project Objective
Develop temperate-adapted maize inbreds
with both anthracnose stalk rot resistance and
good yield potential
from GEM breeding populations
College of Agriculture and Life Sciences
Department of Plant Breeding
Selection and Breeding Scheme
Testcross yield evaluation of these 5 populations(1996)
Selected 4 best yielding 25% exotic : 75% temperate populations
Testcrosses of broader selection of S6 families(2002)
Used both public and Holden's testers
Repeat for S4 families(2000) and
S5 families (2001)
Selfing and selection in S3 families(1999)
Selfed and injected 8 plants/S3 family
Selfing and selection in S2 families(1998)
Selfed and injected 8 plants/S2 family
Repeat for S4 x tester crosses(2000 - 3 locations, 2 reps/loc.)
and S5 x tester crosses(2001 - 2 locations, 2 reps/loc.)
Yield trials of S2 x tester crosses(1999)
3 locations, 2 reps/location
Testcrosses of S2 families(1998)
Crossed to SS or nSS public line cross testers
Selfing and selection in S1 families(1997)
50 S1 families per populationSelfed and injected 8 plants/S1 family
Selfing within the 5 selected populations(1996)
Made about 100 selfs in 25% exotic : 75% temperate populations
ASR screening of GEM Populations(1995)
Selected 5 most resistant
College of Agriculture and Life Sciences
Department of Plant Breeding
Selected Populations
1) FS8B(T):N1802
2) CH04030:S0906
3) AR01150:N0406
4) GOQUEEN:N1603
College of Agriculture and Life Sciences
Department of Plant Breeding
Means by source population for anthracnose stalk rot ratings, 2001
Means of S5 Means of selectedfamily ratings (%) S5 plant ratings (%)
No. Mean No. MeanPopulation Families (± s.d.) Plants (± s.d.) FS8B(T):N1802 6 11.7 (± 1.8) 6 14.0 (± 5.3)CH04030:S0906 9 11.5 (± 1.6) 12 12.6 (± 7.5)AR01150:N0406 6 16.1 (± 4.2) 9 14.5 (± 7.4)GOQUEEN:N1603 9 16.0 (± 6.6) 13 10.6 (± 9.4)
Resis. check (01/31*) 48.0 LB31B 50.6Susc. check (B37) 73.2 * Our most resistant inbred at present, currently being released.
College of Agriculture and Life Sciences
Department of Plant Breeding
Yield Data - 2 locations, 2001Yield Moist. Y/M Lodging (%)
S2 Family Tester (bu/A) (%) Ratio Root Stalk
FS8B(T):N1802-490 B73/CD1 118.5 21.9 5.6 0 19FS8B(T):N1802-491 “ 122.8 21.7 5.8 1 16FS8B(T):N1802-492 “ 119.7 26.0 4.7 0 13FS8B(T):N1802-493 “ 123.5 25.0 5.0 0 16CH04030:S0906-476 RD6501/ 118.5 22.7 5.2 0 45CH04030:S0906-477 RD6502 120.1 21.7 5.6 0 31CH04030:S0906-478 “ 112.5 21.8 5.2 0 24AR01150:N0406-496 B73/CD1 112.9 20.8 5.5 0 43
Pioneer 34G81 166.1 20.0 8.4 8 7Agway 795 128.1 25.0 5.2 0 7Doeblers 596XY 125.3 21.1 6.0 0 15
Coefficient of variation (%) 13.3 5.5
College of Agriculture and Life Sciences
Department of Plant Breeding
Yield Data - 1 location, 2001Yield Moist. Y/M Lodging (%)
S2 Family Tester (bu/A) (%) Ratio Root Stalk
AR01150:N0406-498 B73/CD1 164.1 22.0 7.5 0 3AR01150:N0406-499 “ 187.5 21.8 8.6 0 8GOQUEEN:N1603-510 “ 197.7 20.1 9.9 0 3GOQUEEN:N1603-511 “ 194.0 19.0 10.2 0 6
Pioneer 34G81 214.5 19.6 10.9 0 0Agway 657 193.3 20.5 9.4 0 4Agway 795 183.6 24.0 7.6 0 3Doeblers 596XY 169.5 20.2 8.4 0 3
Coefficient of variation (%) 10.8 4.9
College of Agriculture and Life Sciences
Department of Plant Breeding
Work done in 2002…
• S6 progenies planted ear-to-row– Greater emphasis on yield performance– Greater tolerance of stalk rot resistance
• Testcrosses made to:– Previously used public line crosses– Holden’s testers (LH176/LH177, LH198)