THE CONFUSION ABOUT BT BRINJAL
BYKUTUBUDDIN MOLLA
CSIR JRF
PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY LABORATORY
DEPARTMENT OF BOTANY
UNIVERSITY OF CALCUTTA
PAST SCENARIO Bengal famine in 1943—around 4 million
people died in hunger of eastern India. Green revolution made India self –sufficient
of food grains by 1978 through the adoption of dwarf high yielding hybrid wheat & rice variety.
It was possible due to the use of genetically improved seeds (wheat- rht gene; rice- sd1 gene)
PRESENT SCENARIO Farmland is decreasing.. Population is increasing in a very high rate. Production is not increasing—stagnant. Several problems came out in recent days.– 1) increased temperature 2) salinity 3) drought 4) biotic stresses Our crop is unable to cope up with these new
stresses because they did not face such before.
That’s why traditional breeding is unsuccessful.
ANOTHER WORLDWIDE FAMINE IS WAITING FOR US.!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
So we need a second green revolution. The Process by which we can increase the yield of a
particular crop.
For using genes from non rice sources we have to go for genetic engineering..
Even traditional breeding use the technology(genetic engineering) unknowingly.
SO IN PRESENT SCENARIO FOR A SECOND GREEN REVOLUTION WE HAVE TO GO FOR BIOTECHNOLOGY & GENETIC ENGINEERING
BRINJAL CULTIVATION IN INDIA
MAJOR PROBLEMS
FSB DAMAGE
Shoot damage Fruit Damage
50 - 90% of damage is caused by fruit and shoot borer
Fruit borer Damage as identified by the consumer
WHY WE NEED BT BRINJAL ?? No natural resistance in germplasm Limited success with traditional
management strategies Huge use of pesticides results in
development of resistance in insectsHarmful effects on environment, health of
farmer and consumers Bt brinjal has potential to provide
economic and health benefits
Speculation about Bt or Bt-Brinjal
is like……
The unscientific comments about Bt-Brinjal often reminds us of the six blind men and the elephant who guessed the tail as rope, legs as pole, trunk as wall, ear as fan and the trunk as hose
WE SHOULD KNOW THE ACTUAL & TRUE FACT WHICH IS SCIENTIFIC…………….
WHAT IS BACILLUS THURINGIENSIS (BT)
TM
MB
t cotto
n
EM view of a purified inclusion body
EM view of Bt with spores and crystals
Endospore
Crystal
• Soil bacterium; Soil bacterium; ubiquitousubiquitous
• Different strains produce Different strains produce their their
own insecticide proteins. own insecticide proteins.
•The protein is called as cry The protein is called as cry protein for its crystal form.protein for its crystal form.
• Each Each cry cry protein protein selectively selectively
affects insects belonging affects insects belonging to a to a
particular order particular order (eg.,Lepidoptera, (eg.,Lepidoptera,
Coleoptera, Diptera, etc) Coleoptera, Diptera, etc) at at
their very early larval their very early larval stagestage
HISTORY OF SAFE USE AS A SPRAY Since 1938, used extensively as a spray all
over the world to control a variety of lepidopteron pests and also mosquito larvae.
TM
MB
t cotto
n
IN INDIA
IN THAILAND
IN CALIFORNIA IN INDIA
IN AUSTRALIA
GLOBAL MARKET FOR BIOPESTICIDES, 2005-06 - $260M
TM
MB
t cotto
n
Bt
$159m
Virus
$18m
Fungus
$37m
Nematode
$15m
62%
Other bacteria
$32m
Source: CPL Business Consultants, July 2006
Bt spray dominated with 62%. Used in all countries, including the European Union (16.5%), and by all farmers including organic farmers
HOW BT BRINJAL DEVELOPED?
Bt brinjal is a genetically improved brinjal developed by inserting a cry1Ac gene into brinjal genome
The Cry1Ac protein gives the brinjal plant in-built resistance against lepidopteran insects like Brinjal Fruit and Shoot Borer
Use of Bt as insecticide not new – farmers using it for over 50 years as a spray
PLR1
Non-Bt Bt
SHOOT DAMAGE
Non Bt Bt
FRUIT DAMAGE
Non bt brinjal Bt brinjal
BtBt-Cotton Approved-Cotton Approved
26 March 2002: A Landmark in India26 March 2002: A Landmark in India
India’s first transgenic crop
Bt Cotton
Bt-cotton area & cotton production in India620 Bt-cotton hybrids & 1 Bt variety have been released so far
New Hybrids
Bt Cotton area lakh ha
India: Raw Cotton Imports and Exports India: Raw Cotton Imports and Exports 1991-1991-20092009
Insecticide usage in India
IRM
Bt Cotton
Bollworm insecticides Rs 600 crores in 2002 Bollworm Insecticides Rs 260 crores in 2008
NET RESULT OF BT COTTON APPROVAL INDIA WAS AN IMPORTER OF COTTON
BEFORE BT COTTON DEVELOPED……………..NOW INDIA IS AN EXPORTER.
INDIA IS NOW THE SECOND LARGEST COTTON PRODUCER IN THE WORLD
THE NET INCOME DERIVED BY BT FARMERS WAS RS.26,406 (US$600.13)/HA WHILE IT WAS RS.9,059 (US$205.88)/HA FOR THE NON-BT FARMERS.
NET GAIN IN BT COTTON CULTIVATION IS Rs. 4000 CRORES IN INDIA
ABOUT SAFETY OF BT BRINJAL
The controversy began Questioned the safety of Bt
IngestionIngestion (occurs while feeding plant tissues)(occurs while feeding plant tissues)
SolubilizationSolubilization (Alkalinity)(Alkalinity)
ActivationActivation (pH >9.5) (pH >9.5)
BindingBinding (Specific receptor)(Specific receptor)
InsertionInsertion (Damage gut (Damage gut
wall)wall)
Pore formationPore formation
Cell lysesCell lyses
D e D e
a t ha t h
Selective action of Selective action of Bt Bt in insectsin insects
HOW CRY PROTEINS ACT IN THE INSECT GUT
Fig. 3 Taken from de Maagd RA (2001) Trends in Genetics 17(4), 193-199
DETAILS MODE OF ACTION OF BT PROTEIN
BT HAS NO TOXIC IMPACT IN HIGHER ANIMALS (HUMAN) BECAUSE OF…..
Acidic stomach
Very low pH (∽1.5 in humans)
Absence of required receptors
Bt is safe to non-target organisms-HUMAN
NATIONAL REGULATORY SYSTEMS IN INDIA
Ministry of Environment and forests (MoEF)
Department of Biotechnology (DBT)
There are six competent authorities to handle various issues viz.,
Genetic Engineering Approval Committee (GEAC) Review Committee on Genetic Manipulation (RCGM) Recombinant DNA Advisory Committee (RDAC) Institutional Biosafety Committee (IBSC) State Biotechnology Coordination Committee (SBCC) District Level Committee (DLC)
WHAT ARE THE SAFETY CONCERNS?
Toxicity Allergenicity Out-crossing / Gene flow Effects on non-target organisms Environmental impact
Pest resistanceAll safety issues were addressed before regulatory approval was given
SAFETY TO NON-TARGET ORGANISMS
Feed safety studies were conducted using high dose of Bt-cotton seed-meal / protein on:
FishFish BirdsBirds Mammals - Mammals - goats, buffaloes, cows, rabbits
Honey beesHoney bees EarthwormsEarthworms Biological control agentsBiological control agents (Lacewing, Ladybird beetles etc)(Lacewing, Ladybird beetles etc)
Results revealed no ill effects & Bt is safe !
DOES IT AFFECT BIODIVERSITY??
Any new gene incorporation in a genome increases its genetic diversity.
In nature, every evolution of a new species is due to some gene incorporation through cross pollination..
The idea that Bt brinjal will destroy biodiversity is nonsensical. In fact, since brinjal has no natural resistance (to pests), the introduction of Bt brinjal will help increase genetic diversity.
IS IT A THREAT TO OTHER VARIETIES THROUGH CROSS POLLINATION??
Gene transfer is not an easy phenomenon. It has to be fixed in population through selection pressure. Also, there is no horizontal transfer of the gene — it will not transfer to animals; there is only vertical transfer — from one generation to the next within the same species.
WHAT ARE THE BENEFITS OF BT-BRINJAL.?
Benefits to consumers and society: 1) Consumers will have a choice to buy non-infested,
undamaged and good quality brinjal fruits.
2) High levels of pesticide residues are present in a large number of vegetables that we eat daily. Bt brinjal will offer consumers fruits with almost no or significantly reduced level of pesticide residues, well within the prescribed MRL.
3) It is expected that Bt brinjal may improve marketable yield manifold resulting in availability of more brinjal in the market at affordable price. Krishna and Qaim (2007) predicted a 15% decrease in market price of brinjal at maximum technology adoption.
4) The Indian Institute of Horticultural Research (IIHR) showed that Bt brinjal would help stabilize the market price (IIHR, 2008).
BENEFITS TO FARMERSFarmers are expected to benefit at
multiple levels. Some of these include:
1) Considerable reduction in cost of production by saving on cost of insecticides and lower labor cost as a result of reduced spraying.
2) Manifold increase in yield per unit area by saving fruits from damage caused by FSB.
3) Significant improvement in marketable fruits thereby increasing income per unit area.
4) Reduction in direct exposure to insecticides leading to lesser health problems.
BENEFITS TO ECOLOGY AND ENVIRONMENT
1) Reduction in pesticide residues in soil and water in brinjal fields.
2) Lesser pollution of air and local environment due to decreased use of insecticides.
3) Protection of naturally occurring predators and parasitoids and other beneficial organisms due to reduced use of insecticides.
4) Reduction in soil and ground-water contamination.
5) Safeguarding soil microflora and invertebrates from damage caused by unintended and excessive use of insecticides.
Protesting is their ROTI,, KAPRA aur MAKAN..
That’s why every morning they arise question on a new issue…………….
NOW SOME PEOPLE HAS CHOOSEN A NEW PROFESSION------------ PROTESTING
STRANGE!!
The opponents seem to suggest that Bt-Brinjal kills
everything else except FSB – the target pests!!.....
like the blind people..
SMT . PRATIBHA DEBISINGH PATIL, THE PRESIDENT OF INDIA
“Efforts towards sustainable agriculture can be greatly augmented with the help of space technology and biotechnology advances”
DR. MANMOHAN SINGH, THE PRIME MINISTER OF INDIA
“We need to use the potential of biotechnology to meet the requirements of hungry people”.
MR. P. CHIDAMBARAM, THE UNION HOME MINISTER
“Bt cotton has made India a cotton exporting country. We thought of ourselves as exporters of wheat and rice, but today we import wheat. No country as large as India can survive on imports for its food needs,”….”….”The success achieved in cotton must be used to make the country self sufficient in rice, wheat, pulse and oil seed production”.
The scientists & students should come together and speak in one voice.
Defend the technology – defeat opponents
Silence is not gold. Silencing is!
THANK YOU
HAVE A NICE DAY