Bergamo II Series Multiphoton Microscopes

www.thorlabs.comV 2 . 1

Step 1:Bergamo II Body

Step 9:Objectives & Accessories

Step 10:Epi-Direction

Detectors

Step 2:Motion Controller

Step 14:TransmittedLightImaging(for StandardBodies)

Step 15:Forward-Direction

Detectors

MCM30003-Axis Controller(Required forCondenser inStep 14)

OPX4220PA/Uncaging

VIS/NIR

MCM50005-Axis Controller (Required for Rotating Bodies)

Step 3:Scan Path(s)

Step 4:Secondary Path

Periscope

Step 5:Uncaged PathFiber Launch

Step 6:Primary Reflector/

Dichroic

Step 7:Dichroic Holder

Step 8:Dual Objective

Nosepiece

Step 13:Widefield Viewing Accessories

Step 16:Beam

Conditioning Modules

Step 11:Sample Stages

Step 12:Epi-Fluorescence

OPX1100 PrimaryGalvo/Resonant PathOPX2200 Secondary Galvo/Galvo Path (Dual Scan Paths)

OPX1100Primary Galvo/Resonant Path

(Single Scan Path)

OPX1200 Primary Galvo/Galvo PathOPX2100 Secondary Galvo/Resonant Path(Dual Scan Paths)

OPX1200Primary

Galvo/Galvo Path(Single Scan Path)

FDM22132 Ch

BCM-PCAPockels Cell

DIC Components

BCM-PAVariableAttenuator

BCM-VBEVariable BeamExpander

PMT2100Compact Non-Cooled GaAsP

CSN13022-Position Motorized

Objective Mover

CSN13012-Position ManualObjective Mover

PDM2000Motorized

Dichroic Mover

PDM1000Manual Dichroic

Mover

N16XLWD-PFH N20X-PFH N25X-APO-MP

N40XLWD-NIR N40X-NIR N60X-NIR

WFA2001Single-Cube

Epi-Illuminator

MBE74100 Nikon Epi-Illuminator (Only Compatible w/

BDM1208 and BDM3214 PMT Modules)

FRM1000Silver-Coated Primary Reflector (Single Path Only)

FLC1000405/488/561/705-1600 nm

Polychroic

FRM2000750 nm ShortpassFRM2100810 nm ShortpassFRM2200735 nm LongpassFRM2300680 nm ShortpassFRM24001040/60 Bandpass

WFA4000Nikon Trinoculars w/ Eyepieces & IR Filter

PSF2100Fixed Periscope

PSF2000Fixed Periscope

ForELB4000

ForEMB1000

ForEMB3000

PSA2002ArticulatingPeriscope

PMP1000(/M)Manual 2" Translating XY Platform

PMP-2XY(/M)Motorized 2" Translating

XY Platform

PLS-XY2D 1"

XY Stage

PLS-X1D 1"

X-Axis Stage

Stand & MLS Insert Holder

Stand &Sample Holder

Stand &Slide Holder

WFA5100Single ObjectiveDIC Prism Holder

PI-P725 400 µm

Piezo for Light Objectives

PFM450E450 µm

Piezo for All Objectives

PI-P726100 µm Piezo

for HeavyObjectives

WFA5110CFI175 to

CFI160 DIC Prism Adapter

BDM12082 Ch, 8°

Collection

BDM2210(S)2 Ch, 10°

Collection (w/o or w/ Shutters)

BDM2410(S)4 Ch, 10°

Collection (w/o or w/ Shutters)

BDM3214(S)2 Ch, 14°

Collection (w/o or w/ Shutters)

PMT2000Cooled GaAsP PMT

PMT2100Compact Non-Cooled GaAsP PMT

ELB4050 Elevator Base w/ XYZ + θ Rotating

Body, -50° to +50°(Single Path Only)

ELB4000Elevator Base w/ XYZ + θ Rotating Body, -5° to +95°

EMB1000Z-Axis

Standard Body

X-CITE 200DC-Stabilized Fluorescence

Light Source

High-Power Mounted LEDs

(Only for WFA2001Epi-Illuminator)

HPLS243Plasma

Light Source

1500M-GE1.4 MP Scientific

Camera

Thorlabs Scientific Cameras

NoTrinoculars

Condensers

TrinocularsRecommended

Only forStandard

Bodies

CondenserFocusing

Epi-Illumination Source

WidefieldEpi-Illuminator

8050M-GE8 MP Scientific

Camera

4070M-GE4 MP Scientific

Camera

340M-GEFast Frame Rate Scientific Camera

WFA41020.5X Camera

Port

WFA41010.75X Camera

Port

WFA41001X Camera

PortWFA4105Nikon Y-TV Tube &C-Mount Adapter

WFA4106Nikon Y-TV Tube & 0.7X C-Mount Adapter

WFA4107Nikon TI-3 Tube & C-MountAdapter

WFA4108Nikon TI-3 Tube & 0.7X C-MountAdapter

WFA4002Erect-Image Trinocularsw/ Eyepieces & IR Filter

Nikon MBL78700D-CUO DIC Oil

1.4 NA

Nikon MBL78600D-CUD Universal

0.9 NA

Nikon MBL70100FN-C LWD

0.78 NA

BSA2000Compact

CondenserMounting Arm

CSA2000Condenser

Mounting Arm

ZFM2000Condenser

Focus Module

WFA1000Brightfield/DIC

WFA0100Transmitted Light Module Adapter

LED Drivers

TransmittedLight

IlluminationKits

WFA1100Dodt

Contrast

WFA1010VIS LED

WFA1020NIR LED

WFA1050VIS & NIR LEDs

DC41004 Ch, 1 Mod Input

DC41044 Ch, 4 Mod Input

LEDD1B1 Ch

WFA3000DIC Turret Adapter

Nikon MBD75300FN-PT Polarizer Turret for VIS & IR Polarizers

Nikon MBN71950 FN-IR/FA IR &

VIS DIC Analyzer

Nikon MBH76160DIC Slider 60X

API NIR (N2)

Nikon MBH76720DIC Slider 16X

Nikon MBH76720DIC Slider 25X

Nikon MBN71946FN-P Visible DIC

Nikon MBN71960FN-IRP IR DIC

Nikon MBH72300N2 Dry (10X)

Nikon MBH72310N2 Dry Prism (16-100X)

Nikon MBH76240DIC Slider 40X, Plan Fluor

Nikon MBH76640DIC Slider 40X, APO NIR (N2)

Only for Standard Bodies

Mirror

Dichroics(Others Upon

Request)

Polychroic

Only Available for Standard

Bodies

Body Choice Determines Available Accessories

MultiphotonObjectives

(Others Upon Request)

DIC ObjectivePrism Holder

(StandardBodies Only)

PiezoStages for

Fast Z-Stacks

Epi-DetectionModules

MotionControl

SampleHolders

PMTs w/Preamps

& PSU

PMTs with Preamps & PSU

EMB3000XYZ StandardBody

Laser-Scanned Dodt forGalvo/Res 400-1100 nm

(WFA1130)Galvo/Res 800-1800 nm

(WFA1140) Galvo/Galvo 400-1100 nm

(WFA1150) Galvo/Galvo 800-1800 nm

(WFA1160)

Bergamo II Series Configuration B206This configuration provides Z-axis motion for the objective. It is equipped with trinoculars for widefield viewing, as well as 2 PMTs, extended FOV collection optics, and a galvo-resonant scan path.

Bergamo II Series Configuration B212This configuration offers Z-axis motion for the objective, with an XY platform that moves the experimental apparatus. It is outfitted with trinoculars for widefield viewing, as well as 2 PMTs, full FOV collection optics, and a galvo-galvo scan path.

Bergamo II Series Configuration B248This configuration provides XYZ + θ motion for the objective, with 5" of coarse Z travel and a rotation range of -5° to +95°. It is equipped with a scientific camera for widefield viewing, as well as 4 PMTs, extended FOV collection optics, and dual scan paths.

Bergamo II Series Configuration B264This XYZ configuration is outfitted with

2 forward-direction PMTs and our laser-scanned Dodt contrast module. It

also includes 4 backward-direction PMTs with extended FOV collection optics, dual

scan paths, and a secondary fiber laser input for single-photon applications.

Bergamo II Series Configuration B221This configuration provides XYZ + θ

motion for the objective, with a rotation range of -5° to +95°. It is equipped with a scientific camera for widefield viewing, as

well as 2 PMTs, extended FOV collection optics, and a galvo-resonant scan path.

Bergamo II Series Configuration B244This configuration offers XYZ motion for the

objective. It is outfitted with a scientific camera for widefield viewing, as well as 4 PMTs, extended FOV collection optics, and

dual scan paths.