ISOLATING BACTERIA FROM TROPICAL CONTAMINATED
SOILS IN PUERTO RICO
RISE SPRING 2015
Sara Diaz
Patricia Rodriguez
Camille Ruiz
Justin Cotto
SAMPLING LOCATIONS
Sample Coordinate
s
Environmental
description
Locatio
n
Date/
Time
Ruiz C
- #1
18°6’55.1”N;
66°9’36.2”W
Moist (underneath
grass),
5 cm; 72°F
Cayey,
PR
January/
28/15
7:30 p.m.
Cotto J
- #2
18.15º N
66.18º W
Moist environment
(above grass), 76ºF
Cidra,
PR
January/
29/15
8:00 a.m.
SAMPLING LOCATIONS
Sample Coordinates Environmental
description
Location Date/
Time
Diaz S
-#1
18°15'15.7"N 66°06'06.9"W
Moist environment
80°F
Aguas
Buenas,
P.R.
January/
27/15
4:26 p.m.
Rodríguez P
-#2
18°15'15.7"N 66°06'06.9"W
Moist environment
80°F
Aguas
Buenas,
P.R.
January/
27/15
4:26 p.m.
RESULTS FROM DIFFERENT PROCEDURES
Process CLRV30TSA01A CLRV30M01B JCR30TSA01A
Gram Stain Negative Positive Positive
Bacterial Morphology Coccus Bacillus Bacillus
PCR/Electrophoresis
#1
Negative Negative Negative
PCR/Electrophoresis
#2
Positive Negative -
Antibiotic Production Negative Negative Negative
RESULTS FROM DIFFERENT PROCEDURES
Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01
Gram Stain Positive Positive
Bacterial Morphology Bacillus Bacillus
PCR/Electrophoresis
#1
Negative Negative
PCR/Electrophoresis
#2
Negative Negative
Antibiotic Production Negative Negative
ANTIBIOTIC RESISTANCE TEST RESULTS
Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A
Kanamycin 2.0 cm 1.0 cm 2.0 cm
Penicillin 0.0 cm 0.0 cm -
Tetracycline 1.6 cm 2.4 cm 2.2 cm
Gentamicin 1.9 cm 0.9 cm -
Chloramphenicol 0.0 cm 0.0 cm 2.5 cm
Bacitracin 0.0 cm 0.0 cm 1.5 cm
Vancomycin 0.0 cm 0.0 cm -
Streptomycin 1.6 cm 1.0 cm 1.9 cm
ANTIBIOTIC RESISTANCE TEST RESULTS
Antibiotic S15UPRC-
RISESCDM30T01B
Streptomycin 2.2 cm
Chloramphenicol 2.3 cm
Erythromycin 1.0 cm
Kanamycin 1.6 cm
Gentamicin 0.6 cm
AntibioticS15UPRC-RISEPR30M01
Rifampin 0.13 cm
Kanamycin 0.16 cm
Vancomycin 0.17cm
Streptomycin 0.30 cm
Tetracyclin 0.14 cm