EDGE®
Automated Extraction System
Method Note Compendium
Updated September 25, 2019
Agriculture
Extraction of Pesticides From Avocados 2
Extraction of Polyphenols From Beans 3
Extraction of Polyphenols From Cacao 4
Extraction of Cannabinoids From Cannabis Plant 5
Extraction of Mycotoxins From Cannabis Plant 6
Extraction of Pesticides From Cannabis Plant 7
Extraction of Total Fat From Feeds/Foods 8
Extraction of PCBs From Feeds/Foods (Wet) 9
Extraction of Dioxins From Feeds/Foods (Wet) 10
Extraction of PCBs From Fish Meal (Dry) 11
Extraction of PAHs From Fish Tissue (Dry) 12
Extraction of PAHs From Fish Tissue (Wet) 13
Extraction of Pesticides From Foods (Wet) 14
Extraction of Pesticides From Hops 15
Extraction of Fat From Nuts (Brazil) 16
Extraction of Fat From Nuts (Hazelnuts) 17
Extraction of Fat From Pumpkin Seeds 18
Extraction of Brodifacoum From Rat Poison Powder 19
Extraction of Pesticides From Rice 20
Extraction of PCBs From Soy Meal (Dry) 21
Extraction of Pesticides From Spices (Dry) 22
Extraction of Pesticides From Strawberries 23
Cannabis
Extraction of THC/THCA From Cannabis Flower 25
Extraction of Cannabinoids From Cannabis Plant 26
Extraction of Mycotoxins From Cannabis Plant 27
Extraction of Pesticides From Cannabis Plant 28
ContentsEnvironmental & Regulatory
Extraction of SVOCs Environmental Samples 30
Extraction of SVOCs EPA 3545A 31
Extraction of Dioxins From Calcined Kiln Dust 32
Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 33
Extraction of SVOCs From Environmental Samples 34
Extraction of PAHs From Fish Tissue (Wet) 35
Extraction of Organics From Lake Sediment 36
Extraction of PCBs From PUF Filters (Large) 37
Extraction of PCBs From PUF Filters (Small) 38
Extraction of PAHs From Quartz Air Filter 39
Extraction of Dioxins From Soil 40
Extraction of Diesel and Oils From Soil 41
Extraction of PAHs From Soil, Loam or Clay 42
Extraction of PCBs From Soil, Loam or Clay 43
Extraction of Dioxins From Soil, Loam or Clay 44
Cleaning of PUF Filters 45
Food Testing
Extraction of Pesticides From Avocados 47
Extraction of Polyphenols From Beans 48
Extraction of Fat From Bone Meal 49
Extraction of Fat From Bread 50
Extraction of Polyphenols From Cacao 51
Extraction of Cannabinoids From Cannabis Plant 52
Extraction of Mycotoxins From Cannabis Plant 53
Extraction of Pesticides From Cannabis Plant 54
Extraction of Lipids From Chicken (Baked) 55
Extraction of Lipids From Chicken (Fried) 56
Hydrolysis & Extraction of Total Fat From Chocolate 57
Hydrolysis & Extraction of Total Fat From Cookies 58
Extraction of Fat From DATEM Powder 59
Extraction of Flavonoids from Echinacea 60
Extraction of Fat From Egg Powder 61
Extraction of Total Fat From Feeds/Foods 62
Extraction of PCBs From Feeds/Foods (Wet) 63
Extraction of Dioxins From Feeds/Foods (Wet) 64
Extraction of PCBs From Fish Meal (Dry) 65
Extraction of PAHs From Fish Tissue (Dry) 66
Extraction of PAHs From Fish Tissue (Wet) 67
Extraction of Pesticides From Foods (Wet) 68
Extraction From Goldenseal Root 69
Extraction of Pesticides From Hops 70
Extraction of Total Fat From Hot Dogs 71
Extraction of PAHs From Infant Formula 72
Extraction of Total Fat From Meats (High-Fat) 73
Extraction of Total Fat From Meats (Potted) 74
Extraction of Fat From Nuts (Brazil) 75
Extraction of Fat From Nuts (Cashews) 76
Extraction of Fat From Nuts (Hazelnuts) 77
Extraction of Total Fat From Nuts (Peanuts) 78
Extraction of Total Fat From Pepperoni 79
Extraction of Lipids From Potatoes (Baked) 80
Extraction of Lipids From Potatoes (Fried) 81
Extraction of Nutrients From Protein Powder 82
Extraction of Total Fat From Protein Powder 83
Extraction of Fat From Pumpkin Seeds 84
Extraction of Pesticides From Rice 85
Extraction of PCBs From Soy Meal (Dry) 86
Extraction of Pesticides From Spices (Dry) 87
Extraction From Spirulina Algae 88
Extraction of Pesticides From Strawberries 89
Extraction of PAHs From Yerba Mate 90
Geoscience & Mining
Extraction of Dioxins From Calcined Kiln Dust 92
Extraction of SVOCs, Pesticides, TPH, PCB From Cement Mix 93
Extraction of Molybdenum 94
Industrial Hygiene
Extraction of PCBs From PUF Filters (Large) 96
Extraction of PCBs From PUF Filters (Small) 97
Extraction of Brodifacoum From Rat Poison Powder 98
Cleaning of PUF Filters 99
Materials Science
Extraction of Film Powder 101
Extraction of Pigments From Plastics 102
Extraction of Additives From Polypropylene Powder 103
Extraction of Phthalates From Polyvinyl Chloride 104
Extraction of PCBs From PUF Filters (Large) 105
Extraction of PCBs From PUF Filters (Small) 106
Extraction of Brodifacoum From Rat Poison Powder 107
Extraction of Antioxidants From Resin 108
Extraction of PAHs From XAD Resin 109
Cleaning of PUF Filters 110
Metals & Alloys
Extraction of Molybdenum 112
Nutraceuticals
Extraction of Flavonoids From Echinacea 114
Extraction of PAHs From Fish Tissue (Dry) 115
Extraction of PAHs From Fish Tissue (Wet) 116
Extraction From Goldenseal Root 117
Extraction of PAHs From Infant Formula 118
Extraction of Nutrients From Protein Powder 119
Extraction of Total Fat From Protein Powder 120
Extraction From Spirulina Algae 121
Extraction of PAHs From Yerba Mate 122
Personal Care Products
Extraction of PAHs From Infant Formula 124
Extraction of Mouthwash 125
Extraction of Toothpaste 126
Plastics, Polymers, & Oils
Extraction of Film Powder 128
Extraction of Additives From PET 129
Extraction of Additives From Pigment 130
Extraction of Pigments From Plastics 131
Extraction of Additives From Polyethylene Powder 132
Extraction of Chemical Modifier From Polyethylene Resin 133
Extraction of Additives From Polypropylene Powder 134
Extraction of Phthalates From Polyvinyl Chloride 135
Extraction of Diesel and Oils From Soil 136
Agriculture
1
Extraction of PesticidesFrom AvocadosFrom Avocados
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label2
Extraction of PolyphenolsFrom BeansFrom Beans
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Methanol/Water 15 0:1580
2 (1:1) Methanol/Water 15 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label3
Extraction of PolyphenolsFrom CacaoFrom Cacao
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Methanol/Water 15 0:1580
2 (1:1) Methanol/Water 15 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label4
Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.
SolventsOptions:MethanolIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 9010 0:3055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 30 0:3090
2 Isopropanol 20 0:3090
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label5
Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9C4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9
disc, if M2 used. 2. Weigh the sample into the Q-Cup.
SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:3050
2 Methanol 15 0:3050
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label6
Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:3040
2 1% Acetic Acid in Acetonitrile 15 0:3040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label7
Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight≤ 5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsWet Samples Only: Sand (use equal amount to sample size)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label8
Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13520 2:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label9
Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
SorbentsC18 (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 2:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label10
Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1M1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label11
Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)
Agriculture, Food Testing, NutraceuticalEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label12
Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)
Agriculture, Environmental and RegulatEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (0.8 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:15150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label13
Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label14
Extraction of PesticidesFrom HopsFrom Hops
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 3:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:0040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label15
Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label16
Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 0 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label17
Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label18
Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, MaterialEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 2:001010
2 13015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:15150
2 Methanol 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label19
Extraction of PesticidesFrom RiceFrom Rice
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label20
Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1M1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label21
Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesMatrix matched calibration curve required without the use of sorbents.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.
Solvents1% Acetic Acid in Acetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 3:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label22
Extraction of PesticidesFrom StrawberriesFrom Strawberries
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label23
Cannabis
24
Extraction of THC/THCAFrom Cannabis PlantFrom Cannabis Plant
CannabisEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsOptions:MethanolIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 9010 0:3055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 30 0:3090
2 Isopropanol 20 0:3090
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label25
Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.
SolventsOptions:MethanolIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 9010 0:3055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 30 0:3090
2 Isopropanol 20 0:3090
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label26
Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9C4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9
disc, if M2 used. 2. Weigh the sample into the Q-Cup.
SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:3050
2 Methanol 15 0:3050
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label27
Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:3040
2 1% Acetic Acid in Acetonitrile 15 0:3040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label28
Environmental & Regulatory
29
Extraction of SVOCsEnvironmental SamplesEnvironmental Samples
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesThis is a general method for the miscellaneous extraction of environmental samples.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsOptions:AcetoneDichloromethaneHexaneToluene
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 20 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label30
Extraction of SVOCsEPA 3545AEPA 3545A
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesSee the CEM Application note: Extraction of Semi-Volatile Organic Compounds from Soil in Accordance with EPA 3545A if needed.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Acetone/Hexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Acetone/Hexane 30 0:15150
2 (1:1) Acetone/Hexane 30 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label31
Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust
Environmental and Regulatory, GeoscieEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight10 g
Q-DiscC9 + G1 + C9
EquipmentEDGE60 mL collection vials - amber
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.
SolventsOptions:DichloromethaneMethyl tert-butyl ether
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 2:0000
2 15020 2:0000
3 15020 2:0000
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 20 0:30150
2 Methyl tert-butyl ether 20 0:30150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label32
Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix
Environmental and Regulatory, GeoscieEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight15 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Acetone/Dichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Acetone/Dichloromethane 15 0:30150
2 (1:1) Acetone/Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label33
Extraction of SVOCsFrom Environmental SamplesFrom Environmental Samples
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesThis is a general method for the miscellaneous extraction of environmental samples.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
SolventsOptions:Acetone DichloromethaneHexaneToluene
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 20 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label34
Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)
Agriculture, Environmental and RegulatEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (0.8 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:15150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label35
Extraction of OrganicsFrom Lake SedimentFrom Lake Sediment
Environmental and Regulatory, Organic EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, grind, or cryomill sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
Solvents(1:1) Methanol/Dichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 2:0000
2 10020 2:0000
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Methanol/Dichloromethane 15 0:20150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label36
Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 3:00155
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label37
Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 0:10100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label38
Extraction of PAHsFrom Quartz Air FilterFrom Quartz Air Filter
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight3 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Add the sample on top of the sorbent. No mixing is required.
Solvents(1:1) Hexane/Dichloromethane
SorbentsSand (3 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 2:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Hexane/Dichloromethane 15 0:15100
2 (1:1) Hexane/Dichloromethane 15 0:00100
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label39
Extraction of DioxinsFrom SoilFrom Soil
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight10 g
Q-DiscC1
EquipmentEDGE60 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsOptions:DichloromethaneMethy tert-butyl etherToluene
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 1:0000
2 15020 1:0000
3 15020 1:0000
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 30 0:30150
2 Extraction Solvent 30 0:30150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label40
Extraction of Diesel and OilsFrom SoilFrom Soil
Environmental and Regulatory, Plastics,EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.
SolventsDichloromethane
SorbentsDiatomaceous Earth (1 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 2:0000
2 10010 2:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label41
Extraction of PAHsFrom Soil, Loam or ClayFrom Soil, Loam or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Acetone/Hexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 1:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Acetone/Hexane 15 0:30150
2 (1:1) Acetone/Hexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label42
Extraction of PCBsFrom Soil, Loam or ClayFrom Soil, Loam or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight30 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Acetone/Hexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 1:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Acetone/Hexane 15 0:30150
2 (1:1) Acetone/Hexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.d) Bring to volume or evaporate as necessary for analysis.
label43
Extraction of DioxinsFrom Soil, Loam or ClayFrom Soil, Loam or Clay
Environmental and RegulatoryEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE60 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 1:0000
2 15020 1:0000
3 15015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Toluene 30 0:30150
2 Toluene 30 0:30150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label44
Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsOptions:HexaneAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11510 3:00100
2 11510 3:00100
3 11530 3:00100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 15 0:15150
2 Acetone 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label45
Food Testing
46
Extraction of PesticidesFrom AvocadosFrom Avocados
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)C18 (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label47
Extraction of PolyphenolsFrom BeansFrom Beans
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Methanol/Water 15 0:1580
2 (1:1) Methanol/Water 15 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label48
Extraction of FatFrom Bone MealFrom Bone Meal
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight10 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sorbent in the Q-Cup.
SolventsPetroleum Ether
SorbentsDiatomaceous Earth (5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroluem Ether 15 0:15150
2 Petroluem Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label49
Extraction of FatFrom BreadFrom Bread
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight4 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Dry the sample and mill, or blend, to a fine powder.3. Weigh the sample into the Q-Cup.
SolventsPetroleum Ether
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label50
Extraction of PolyphenolsFrom CacaoFrom Cacao
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 8020 10:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Methanol/Water 15 0:1580
2 (1:1) Methanol/Water 15 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label51
Extraction of CannabinoidsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.
SolventsOptions:MethanolIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 9010 0:3055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 30 0:3090
2 Isopropanol 20 0:3090
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label52
Extraction of MycotoxinsFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9C4
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.Either C4 or M2/C9 Q-Disc can be used.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layr the M2 disc (textured sided up) on top of the C9
disc, if M2 used. 2. Weigh the sample into the Q-Cup.
SolventsOptions:Methanol May also use a percentage of Water/Methanol as needed
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 5015 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:3050
2 Methanol 15 0:3050
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label53
Extraction of PesticidesFrom Cannabis PlantFrom Cannabis Plant
Agriculture, Cannabis, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1.5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesPossessing, using, distributing, or selling marijuana or marijuana-based products constitute federal crimes in the United States, even where a state law decriminalizes or legalizes such activities. CEM Corporation produces instruments that are intended for use in testing laboratories and applications only where such use is permitted under applicable state/country law.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsContents of Agilent QuEChERS SPE 15 mL Tube
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 2:0055
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:3040
2 1% Acetic Acid in Acetonitrile 15 0:3040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label54
Extraction of LipidsFrom Chicken (Baked)From Chicken (Baked)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight9 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
Solvents(2:1) Chloroform/Methanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 3030 4:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (2:1) Chloroform/Methanol 30 0:2030
2 (2:1) Chloroform/Methanol 30 0:0030
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label55
Extraction of LipidsFrom Chicken (Fried)From Chicken (Fried)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight9 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
Solvents(2:1) Chloroform/Methanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 3030 4:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (2:1) Chloroform/Methanol 15 0:2030
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label56
Hydrolysis & Extraction of Total FatFrom ChocolateFrom Chocolate
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.
ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.
SolventsExtraction:Petroleum Ether(25:11) Concentrated Hydrochloric Acid/WaterWash:Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Water 15 0:00100
2 Petroleum Ether 15 0:15100
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label57
Hydrolysis & Extraction of Total FatFrom CookiesFrom Cookies
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesStep 2- EDGE Method1. Assemble the Q-Cup with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Place the Filter Paper into the Q-Cup.
ProcedureStep 1- Hydrolysis Method 1. Weigh out 1 g of milled or ground sample. 2. Add 1 mL Ethanol and 5 mL (25:11) Concentrated Hydrochloric Acid:Water. Heat for 1 hr at 100° C in a covered dish. 3. Add 5 mL of Ethanol. Filter the sample using Whatman 3 Filter Paper with a filter funnel, a vacuum pump, and filter flask. Wash any residual material from the beaker in the filter funnel using Ethanol. 4. Dry the filter paper in an oven at 100° C for 30 minutes. After the drying step, place Filter Paper into the Q-Cup® and extract.
SolventsExtraction:Petroleum Ether(25:11) Concentrated Hyrdochloric Acid/WaterWash:Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Water 15 0:00150
2 Petroleum Ether 15 0:15150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label58
Extraction of FatFrom DATEM PowderFrom DATEM Powder
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.3. Weigh the sand directly on top of the sample to compact down.
SolventsPetroleum Ether
SorbentsSand (1 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10010 1:0000
2 10010 1:0000
3 10010 1:0000
4 1005 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroluem Ether 15 0:15150
2 Petroluem Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
59
Extraction of FlavonoidsFrFromom Echinacea
Food TestingEDGE
label
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of theC9 Q-Disc.2. Weigh the sample into the Q-Cup.
Solvents(70:30) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 6020 1:00020
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (70:30) Methanol/Water 15 0:1060
2 (70:30) Methanol/Water 10 0:0060
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtainrequired results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position andload method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label 60
Extraction of FatFrom Egg PowderFrom Egg Powder
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsToluene
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Toluene 15 0:15150
2 Toluene 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label61
Extraction of Total FatFrom Feeds/FoodsFrom Feeds/Foods
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight≤ 5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with sample and sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsWet Samples Only: Sand (use equal amount to sample size)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label62
Extraction of PCBsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsDichloromethane
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13520 2:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label63
Extraction of DioxinsFrom Feeds/Foods (Wet)From Feeds/Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC4
EquipmentEDGE60 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:DichloromethaneMethyl tert-butyl etherToluene
SorbentsC18 (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 1:0000
2 15020 1:0000
3 15020 1:0000
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 30 0:30150
2 Extraction Solvent 30 0:30150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label64
Extraction of PCBsFrom Fish Meal (Dry)From Fish Meal (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1M1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18: Sample.Either C1 or M1/C9 Q-Disc can be used.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using the M1 disc, layer a C9 disc on top of M1.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label65
Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)
Agriculture, Food Testing, NutraceuticalEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label66
Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)
Agriculture, Environmental and RegulatEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (0.8 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:15150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label67
Extraction of PesticidesFrom Foods (Wet)From Foods (Wet)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbents. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label68
ExtractionFrom Goldenseal RootFrom Goldenseal Root
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetonitrile 15 0:15150
2 Acetonitrile 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label69
Extraction of PesticidesFrom HopsFrom Hops
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesCanadian Pesticide Mix 4 in LCMS grade obtained from SPEX CertiPrep.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Add sorbents to bottom of Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. No mixing is required.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is required.4. Spike with 100 ug/mL Canadian Pesticide Mix 4 and leave in hood for approximately 30 minutes to evaporate solvent.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (1.25 g)Supelᵀᴹ QuE Citrate (1.25 g)Primary secondary amine (1.25 g)Siliabond C18 (1.25 g)*Different sorbents can be used
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 3:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:0040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label70
Extraction of Total FatFrom Hot DogsFrom Hot Dogs
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsExtraction:Petroleum EtherWash:Water
SorbentsSand (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Water 10 0:00150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label71
Extraction of PAHsFrom Infant FormulaFrom Infant Formula
Food Testing, Nutraceuticals, Personal EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight4 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsCyclohexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 15 0:30150
2 Cyclohexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label72
Extraction of Total FatFrom Meats (High-Fat)From Meats (High-Fat)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label73
Extraction of Total FatFrom Meats (Potted)From Meats (Potted)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsExtraction:Petroleum EtherWash:Water
SorbentsSand (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Water 10 0:00150
2 Petroleum Ether 15 0:15150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label74
Extraction of FatFrom Nuts (Brazil)From Nuts (Brazil)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label75
Extraction of FatFrom Nuts (Cashews)From Nuts (Cashews)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label76
Extraction of FatFrom Nuts (Hazelnuts)From Nuts (Hazelnuts)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 0 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label77
Extraction of Total FatFrom Nuts (Peanuts)From Nuts (Peanuts)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label78
Extraction of Total FatFrom PepperoniFrom Pepperoni
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.4. Dry Q-Cups with the sample and the sand in the oven for 1 hour at 100° C while tilted at an angle in ~2" aluminum pans or equivalent. This provides stability and enables easier visual identification that no sample has been lost.
SolventsPetroleum Ether
SorbentsSand (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14010 1:00010
2 14010 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Water 10 0:00150
2 Petroleum Ether 15 0:15150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label79
Extraction of LipidsFrom Potatoes (Baked)From Potatoes (Baked)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight9 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
Solvents(3:2) Hexane/Isopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 3030 4:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (3:2) Hexane/Isopropanol 30 0:2030
2 (3:2) Hexane/Isopropanol 30 0:0030
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label80
Extraction of LipidsFrom Potatoes (Fried)From Potatoes (Fried)
Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight9 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
Solvents(3:2) Hexane/Isopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 3030 4:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (3:2) Hexane/Isopropanol 15 0:2030
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label81
Extraction of NutrientsFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
Solvents(80:20) Methanol/Water
SorbentsSand (3 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 0:101010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (80:20) Methanol/Water 15 0:15150
2 (80:20) Methanol/Water 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label82
Extraction of Total FatFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (6 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 2:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label83
Extraction of FatFrom Pumpkin SeedsFrom Pumpkin Seeds
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight12 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (12 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14020 1:0000
2 14015 1:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label84
Extraction of PesticidesFrom RiceFrom Rice
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1540
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label85
Extraction of PCBsFrom Soy Meal (Dry)From Soy Meal (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1M1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesLess than 5 grams of sample can be used with a ratio of 1:2 C18/Sample.Either C1 or M1/C9 Q-Disc can be used.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. If using M1 disc, layer on top of C9 disc.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (2.5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:15150
2 Extraction Solvent 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label86
Extraction of PesticidesFrom Spices (Dry)From Spices (Dry)
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesMatrix matched calibration curve required without the use of sorbents.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sample into the Q-Cup.
Solvents1% Acetic Acid in Acetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4015 3:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label87
ExtractionFrom Spirulina AlgaeFrom Spirulina Algae
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.
2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetonitrile 15 0:00150
2 Acetonitrile 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label88
Extraction of PesticidesFrom StrawberriesFrom Strawberries
Agriculture, Food TestingEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between 2 C9 Q-Disc. 2. Weigh the sorbents into the Q-Cup. Spread evenly across the sample by gentle tapping.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
Solvents1% Acetic Acid in Acetonitrile
SorbentsQ-Matrix Hydraᵀᴹ (2.5 g)Primary Second Amine (2 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 1% Acetic Acid in Acetonitrile 15 0:1040
2 1% Acetic Acid in Acetonitrile 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label89
Extraction of PAHsFrom Yerba MateFrom Yerba Mate
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 120-15015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:30150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label90
Geoscience & Mining
91
Extraction of DioxinsFrom Calcined Kiln DustFrom Calcined Kiln Dust
Environmental and Regulatory, GeoscieEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight10 g
Q-DiscC9 + G1 + C9
EquipmentEDGE60 mL collection vials - amber
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup®. Sandwich the G1 Q-Disc® between the two C9 Q-Discs. 2. Weigh the sample into the Q-Cup.
SolventsOptions:DichloromethaneMethyl tert-butyl ether
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 2:0000
2 15020 2:0000
3 15020 2:0000
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 20 0:30150
2 Methyl tert-butyl ether 20 0:30150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label92
Extraction of SVOCs, Pesticides, TPH, PCBFrom Cement MixFrom Cement Mix
Environmental and Regulatory, GeoscieEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight15 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs. 2. Weigh the sample into the Q-Cup.
Solvents(1:1) Acetone/Dichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Acetone/Dichloromethane 15 0:30150
2 (1:1) Acetone/Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label93
Extraction ofMolybdenumMolybdenum
Geoscience and Mining, Metals and AllEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetone 15 0:15140
2 Acetone 15 0:00140
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label94
Industrial Hygiene
95
Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 3:00155
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label96
Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 0:10100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label97
Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, MaterialEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 2:001010
2 13015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:15150
2 Methanol 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label98
Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsOptions:HexaneAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11510 3:00100
2 11510 3:00100
3 11530 3:00100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 15 0:15150
2 Acetone 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label99
Materials Science
100
Extraction ofFilm PowderFilm Powder
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.
SolventsOptions:CyclohexaneDichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-7015 10:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 15 0:0070
2 Dichloromethane 15 0:1570
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label101
Extraction of PigmentsFrom PlasticsFrom Plastics
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.
SolventsOptions:CyclohexaneDichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-8015 10:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 10 0:1580
2 Dichloromethane 10 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label102
Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12030 15:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Isopropanol 15 0:15150
2 Isopropanol 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label103
Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup
Solvents(1:1) Isopropanol/Cyclohexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *8020 10:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Isopropanol/Cyclohexane 10 0:1580
2 (1:1) Isopropanol/Cyclohexane 10 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label104
Extraction of PCBsFrom PUF Filters (Large)From PUF Filters (Large)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11520 3:00155
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label105
Extraction of PCBsFrom PUF Filters (Small)From PUF Filters (Small)
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsHexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 0:10100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 10 0:15150
2 Hexane 10 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label106
Extraction of BrodifacoumFrom Rat Poison PowderFrom Rat Poison Powder
Agriculture, Industrial Hygiene, MaterialEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 13015 2:001010
2 13015 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:15150
2 Methanol 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label107
Extraction of AntioxidantsFrom ResinFrom Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsMethanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 2:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Methanol 15 0:20150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label108
Extraction of PAHsFrom XAD ResinFrom XAD Resin
Materials ScienceEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight15 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesBe sure that sample or sand is present in the Q-Cup when running this method. Sample size does matter, as you decrease the sample size you will build up more pressure within the cell.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsDichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15020 2:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label109
Cleaning ofPUF FiltersPUF Filters
Environmental and Regulatory, IndustriaEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample WeightEntire Filter
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Assemble the Q-Cup® with the Q-Disc®. 2. Compress and push the filter into the base of the Q-Cup.
SolventsOptions:HexaneAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 11510 3:00100
2 11510 3:00100
3 11530 3:00100
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 15 0:15150
2 Acetone 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label110
Metals & Alloys
111
Extraction ofMolybdenumMolybdenum
Geoscience and Mining, Metals and AllEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 14015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetone 15 0:15140
2 Acetone 15 0:00140
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label112
Nutraceuticals
113
Extraction of FlavonoidsFrom EchinaceaFrom Echinacea
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscM2 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the M2 Q-Disc (textured side up) on top of the C9 Q-Disc.2. Weigh the sample into the Q-Cup.
Solvents(70:30) Methanol/Water
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 6020 1:00020
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (70:30) Methanol/Water 15 0:1060
2 (70:30) Methanol/Water 10 0:0060
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label114
Extraction of PAHsFrom Fish Tissue (Dry)From Fish Tissue (Dry)
Agriculture, Food Testing, NutraceuticalEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sorbent in the Q-Cup. Tap lightly to spread evenly.3. Weigh the sample into the Q-Cup layering the sample on top of the sorbent. No mixing is required.
SolventsDichloromethane
SorbentsC18 (1.7 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label115
Extraction of PAHsFrom Fish Tissue (Wet)From Fish Tissue (Wet)
Agriculture, Environmental and RegulatEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesIf there is moisture in collection vial after the extraction:Using a glass funnel with glass wool and about 20 g of Sodium Sulfate. Pour over into new collection vial and rinse thoroughly with fresh solvent.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsOptions:Dichloromethane(1:1) Hexane/Acetone
SorbentsC18 (0.8 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 3:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:15150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label116
ExtractionFrom Goldenseal RootFrom Goldenseal Root
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetonitrile 15 0:15150
2 Acetonitrile 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label117
Extraction of PAHsFrom Infant FormulaFrom Infant Formula
Food Testing, Nutraceuticals, Personal EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight4 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsCyclohexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 15 0:30150
2 Cyclohexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label118
Extraction of NutrientsFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC4
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
Solvents(80:20) Methanol/Water
SorbentsSand (3 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 15015 0:101010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (80:20) Methanol/Water 15 0:15150
2 (80:20) Methanol/Water 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label119
Extraction of Total FatFrom Protein PowderFrom Protein Powder
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup. Gently mix the sample and the sand in the Q-Cup.
SolventsPetroleum Ether
SorbentsSand (6 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12015 2:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Petroleum Ether 15 0:15150
2 Petroleum Ether 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label120
ExtractionFrom Spirulina AlgaeFrom Spirulina Algae
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight2 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®.
2. Weigh the sample into the Q-Cup.
SolventsAcetonitrile
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Acetonitrile 15 0:00150
2 Acetonitrile 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label121
Extraction of PAHsFrom Yerba MateFrom Yerba Mate
Food Testing, NutraceuticalsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.5 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsOptions:(1:1) Hexane/AcetoneDichloromethaneAcetone
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 120-15015 1:00105
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Extraction Solvent 15 0:30150
2 Extraction Solvent 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label122
Personal Care Products
123
Extraction of PAHsFrom Infant FormulaFrom Infant Formula
Food Testing, Nutraceuticals, Personal EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight4 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between two C9 discs.2. Weigh the sample into the Q-Cup.
SolventsCyclohexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12020 1:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 15 0:30150
2 Cyclohexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label124
Extraction ofMouthwashMouthwash
Personal Care (Health and Beauty)EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs.
2. Weigh the sorbent into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping. 3. Weigh the sample into the Q-Cup layering on top of the sorbent. No mixing is needed.
SolventsHexane
SorbentsOptions:Sodium Polyacrylate (5 g)Diatomaceous Earth (5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 15 0:15100
2 Hexane 15 0:00100
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label125
Extraction ofToothpasteToothpaste
Personal Care (Health and Beauty)EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*for sand- mix into sample*for Diatamaceous Earth- layer under sample, do not mix.
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent/sand into the Q-Cup. Spread evenly across the base of the Q-Cup by gentle tapping.3. Weigh the sample into the Q-Cup*.
SolventsHexane
SorbentsOptions:Sand (15 g)Diatamaceous Earth (5 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 4020 3:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Hexane 15 0:1540
2 Hexane 15 0:0040
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label126
Plastics, Polymers, & Oils
127
Extraction ofFilm PowderFilm Powder
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.
SolventsOptions:CyclohexaneDichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-7015 10:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 15 0:0070
2 Dichloromethane 15 0:1570
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label128
Extraction of AdditivesFrom PETFrom PET
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone/Hexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 115*25 5:00510
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (40:60) Acetone/Hexane 15 0:15150
2 (40:60) Acetone/Hexane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label129
Extraction of AdditivesFrom PigmentFrom Pigment
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
SolventsDimethylformamide
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 1:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dimethylformamide 10 0:15125
2 Dimethylformamide 10 0:00125
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label130
Extraction of PigmentsFrom PlasticsFrom Plastics
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Layer the M1 disc on top of the C9 disc. 2. Weigh the sample into the Q-Cup.
SolventsOptions:CyclohexaneDichloromethane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *40-8015 10:0005
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Cyclohexane 10 0:1580
2 Dichloromethane 10 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label131
Extraction of AdditivesFrom Polyethylene PowderFrom Polyethylene Powder
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscM1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Layer the C9 Q-Disc on top of the M1 Q-Disc. 2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *9030 15:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Isopropanol 15 0:1590
2 Isopropanol 15 0:0090
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label132
Extraction of Chemical ModifierFrom Polyethylene ResinFrom Polyethylene Resin
Plastics, Polymers, and OilsEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight0.1 g
Q-DiscC1
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. 2. Weigh the sample into the Q-Cup.
Solvents(40:60) Acetone/Hexane
SorbentsNone
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *6020 15:001010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (40:60) Acetone/Hexane 10 0:1560
2 (40:60) Acetone/Hexane 10 0:0060
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label133
Extraction of AdditivesFrom Polypropylene PowderFrom Polypropylene Powder
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sample into the Q-Cup.
SolventsIsopropanol
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 12030 15:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Isopropanol 15 0:15150
2 Isopropanol 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label134
Extraction of PhthalatesFrom Polyvinyl ChlorideFrom Polyvinyl Chloride
Materials Science, Plastics, Polymers, aEDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight1 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
Notes*Plastics and polymers should only be extracted at temperatures below melting point range.Expansion of the sample during extraction is common during polymer based extraction.
Procedure 1. Homogenize, mill, or cryomill the sample. Assemble the Q-Cup® with the Q-Disc. Sandwich the G disc between 2 C9 discs. 2. Weigh the sample into the Q-Cup
Solvents(1:1) Isopropanol/Cyclohexane
SorbentsN/A
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 *8020 10:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 (1:1) Isopropanol/Cyclohexane 10 0:1580
2 (1:1) Isopropanol/Cyclohexane 10 0:0080
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label135
Extraction of Diesel and OilsFrom SoilFrom Soil
Environmental and Regulatory, Plastics,EDGE
Note: Temperature and hold times may vary depending on the sample and analytes of interest.
Heating Program
Sample Weight5 g
Q-DiscC9 + G1 + C9
EquipmentEDGE40 mL collection vials - clear
NotesNone
Procedure1. Assemble the Q-Cup® with the Q-Disc®. Sandwich the G1 disc between the two C9 discs. 2. Weigh the sorbent into the Q-Cup.3. Weigh the sample into the Q-Cup on top of the sorbent. No mixing is required.
SolventsDichloromethane
SorbentsDiatomaceous Earth (1 g)
Wash Program
Cycle Temp (°C)Top Add (mL) Hold (mm:ss)Rinse (mL)Bottom Add (mL)
1 10020 2:0000
2 10010 2:00010
Cycle Solvent Volume (mL) Hold (mm:ss)Temp (°C)
1 Dichloromethane 15 0:30150
2 Dichloromethane 15 0:00150
General Guidelinesa) This procedure is a reference point for sample extraction using a CEM system and may need to be modified or changed to obtain required results on your sample.b) Wear hand, eye, and body protection when handling organic solvents.c) Any of CEM's collection vials/centrifuge tubes in the correct size for the rack owned may be used.d) Verify needed solvents are loaded onto the system with sufficient volume. Load the sample rack into the EDGE. Select position and load method. Press play and add any any sample ID's, etc. as needed.e) Bring to volume or evaporate as necessary for analysis.
label136