Anti-Importin 3
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
Anti-Importin 1 Anti-Importin 1
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
Anti-E47
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
A B
C
D
Supplementary figure 1
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
E
Anti-NeuroD1
* *
*
**
Anti-GAPDH
NIH
3T3
cells
Prim
ary
hipp
ocam
pal n
euro
ns
Mou
se E
S c
ells
F
Flag-NeuroD1
Flag-134-161
Anti-flag DAPI
Supplementary figure 2
GFP Injection Marker
NeuroD1-GFP
NeuroD1-GFP + RanQ69L-GTP
HeLa
A B
C D
Supplementary figure 3
NIH3T3
E F
HG
GFP Injection Marker
Supplementary figure 4
Anti-RanBP5 Anti-RanBP7
Neu
roD
1-G
ST
+cyt
osol
Neu
roD
1-G
ST
+cyt
osol
+R
anQ
69L
-
GTPHel
a ce
ll ly
sate
GS
T be
ads+
Cyt
osol
Neu
roD
1-G
ST
+cyt
osol
Neu
roD
1-G
ST
+cyt
osol
+R
anQ
69L
-
GTPHel
a ce
ll ly
sate
GS
T be
ads+
Cyt
osol
75100
150
A B
Supplementary figure 5
Importin 1 Importin + 1
(NeuroD1)
(A)
Database : NCBInr 20100904 (11744690 sequences; 4010973687 residues)Taxonomy : Homo sapiens (human) (232987 sequences)Timestamp : 8 Sep 2010 at 09:45:05 GMTTop Score : 182 for gi|19923142, importin subunit beta-1 [Homo sapiens]
Mascot Score Histogram
Protein score is -10*Log(P), where P is the probability that the observed match is a random event.Protein scores greater than 66 are significant (p<0.05)
Search Parameters
Enzyme : TrypsinFixed modifications : Carbamidomethyl (C)Variable modifications : Oxidation (M)Mass values : MonoisotopicProtein Mass : 95 kDaPeptide Mass Tolerance : ± 0.2 DaPeptide Charge State : 1+Max Missed Cleavages : 1Number of queries : 36
(B)
Supplementary figure 6