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Supplementary Fig. 2A Supplementary Fig. 2B

Med or E2 treatment prevented Ovx-induced loss of CD28 on the BM as well as in spleen CD8+ T cells. (A) CD8+CD28+ T cells in BM, (B) CD8+CD28+ T cells in spleen of Sham, Ovx, Med and E2 were measured by flow cytometry as described in Materials and Methods. N = 10 mice/group; data are presented as mean SEM, **P < 0.01, ***P < 0.001 compared with Ovx + vehicle group.