100
General Background
100 100 100 100
200 200 200 200 200
Tetra Environment
Characteristics of Tetra
ASSET Labs and
Tetra
ASSET Lab Equipment
101
300 300 300 300 300
400
500
400 400 400 400
500500500 500500
400
300
200
100
ASSET 101
Final Jeopardy
Tetrahymena is a ______ cell, meaning it has a membrane-bound
nucleus and organelles.
What is a Eukaryote? What is a Eukaryote?
This is the Kingdom that Tetrahymena belong to.
What is Protista?What is Protista?
This is the term used to describe something that DOES NOT cause
disease. It is also one of the reasons Tetrahymena is a safe organism to use
in classrooms.
What is non-What is non-pathogenic?pathogenic?
Like other living things, Tetrahymena perform these three
basic functions…
What are eat, reproduce, and What are eat, reproduce, and respond to their respond to their environment?environment?
In 2009, this award was given to scientists doing research on telomeres using Tetrahymena.
What is the Nobel Prize?What is the Nobel Prize?
Tetrahymena are found mostly in this type of water…
What is fresh water?What is fresh water?
This is Tetrahymena’s food of choice, another reason why they
are an excellent classroom organisms for non-sterile
environments.
What are bacteria?What are bacteria?
Like all organisms, Tetrahymena ___________, for limited resources
in their environment.
(Charles Darwin identified this as a basic biological concept)
What is compete?What is compete?
Depending on environmental conditions, certain strains of Tetrahymena can go _______,
meaning they eat their own species.
What is Cannibalistic?What is Cannibalistic?
When Tetrahymena go cannibalistic, the cannibals
form one of these, which means, “Big Mouth”.
What is a macrostome?What is a macrostome?
Tetrahymena belong to this group of Protozoans, meaning
they have cilia.
What are Ciliates?What are Ciliates?
This is the term used to describe how Tetrahymena eat. It means “to devour” a
particle when feeding.
What is Phagocytosis?What is Phagocytosis?
“Fat”, “Balloon” and “Mouthless” are three different
______________ that are expressed when Tetrahymena are
exposed to increased temperature.
(37 degrees C)
What are Mutations?What are Mutations?
One of the ASSET Modules allows student to create microcosms where they mix one of Tetrahymena’s favorite bacteria with the Tetrahymena. Bacteria that are able to survive by filling different niches produce visibly different colonies. The selection pressure causing this is?
What is Predation?
Humans have 2 sexes. How many sexes do Tetrahymena
have?
What are Seven (7)?What are Seven (7)?
These are structures found on the outside of Tetrahymena
used for both movement and feeding.
What are Cilia?What are Cilia?
This is the shape that Tetrahymena form when they are sexually reproducing. Also, a common term for a throwing device traditionally use by Australia’s Aboriginal people who use them for hunting.
What is a boomerang?
These organelles can be seen clearly under a microscope during the
Phagocytosis and Osmosis Labs. Their role is to fill-up with extra water and
pump it out of the cell to maintain homeostasis.
What are contractile What are contractile vacuoles?vacuoles?
In the Field Research and Bioinformatics Lab, your students can extract Tetrahymena’s ________ and
compare it to others to identify whether it is from a currently known species of
Tetrahymena or a new species.
What is DNA ?What is DNA ?
This refers to an organism’s ability to change directions in response to chemicals in its
environment. Also, the name of one of the ASSET Labs.
What is Chemotaxis ?What is Chemotaxis ?
These devices ship with many of the ASSET Lab Modules,
they are designed to precisely measure, in microliters, the
volume of a substance.
What are micropipettes?What are micropipettes?
These are the types of microscopes, with two lenses,
you will need to use when observing Tetrahymena in
your classroom.
What are compound light What are compound light microscopes?microscopes?
When culturing Tetrahymena in your classroom, it is important to use ________ technique, which is why we provide you with pipettes
that are individual wrapped.
What is STERILE technique?What is STERILE technique?
We lend these devices (standard and mini sizes),
which allow you to spin down cells using centripetal
acceleration.
What is a Centrifuge What is a Centrifuge (Micofuge)?(Micofuge)?
These devices allow students to work in groups and all be
engaged in watching what is going on under the microscope. They are a great technological
tool to integrate into your science classroom!
What is a Digital Camera What is a Digital Camera
(ASSET uses Moticams)?(ASSET uses Moticams)?
When asking to borrow an ASSET Lab Module, you must first
__________ on our website?
What is to What is to registerregister (create (create an easy to remember user an easy to remember user
name and password)?name and password)?
These two fantastic people started the ASSET Program
at Cornell University.
Who are Donna Cassidy-Who are Donna Cassidy-Hanley, and Ted Clark?Hanley, and Ted Clark?
When borrowing an ASSET module, we ask for this many
weeks notice.
What are Two Weeks?What are Two Weeks? PLEASE!PLEASE!
When your ASSET Kit arrives, what is the first thing you should do after opening
it?
What is find your What is find your TetrahymenaTetrahymena shipping tube shipping tube and make a Stock Culture and make a Stock Culture of it using the falcon tube of it using the falcon tube
provided?provided?
Three (3) days before running a lab with students, you will need to make
“working cultures” of Tetrahymena in the provided sterile petri plates, HOW LONG do these plates generally stay
viable to use in experiments?
What are 3-4 days?What are 3-4 days?(Plates start to crash on about Day 5. (Plates start to crash on about Day 5.
Plan to make fresh plates if you are Plan to make fresh plates if you are using with other classes later in the using with other classes later in the
week.)week.)
Category:Category:
Cellular BiologyCellular Biology
How much will you wager?
TetrahymenaTetrahymena have a ______, that have a ______, that contains 5 pairs of chromosomes with contains 5 pairs of chromosomes with
about the same number of genes as about the same number of genes as humans have and divides mitotically. humans have and divides mitotically.
TetrahymenaTetrahymena also have a ________, also have a ________, which have about 300 chromosomes which have about 300 chromosomes and instead of dividing mitotically, it and instead of dividing mitotically, it
elongates, constricts and divides to pass elongates, constricts and divides to pass on to the daughter cells.on to the daughter cells.
((NEED BOTH BLANKS for Full Points)NEED BOTH BLANKS for Full Points)
What are a micronucleus and a macronucleus ?
Tetrahymena have 2 nuclei! COOL!