To investigate how to determine the Biomass of Chestnut treesTo review hypovirulence in Chestnut Blight

PowerPoint reviewingTreatment of CankersDNA marker & restriction enzymes Chestnut tree history Soup Lab

Read the above article by Dennis Fullbright and answer the questions providedChestnut Blight ArticleQuestions for Blight Article

Read the article in Forest & Ecology Management and answer the following questions:What can measuring biomass tell us about Chestnut trees?Explain the results of the studyOver the time period of a year-what will a beginning and ending biomass measurement be able to tell you about your trees?

The website below is the article listed on the prior page http://www.agriculture.purdue.edu/fnr/htirc/pdf/publications/Jacobs%20et%20al.%202009.pdf

Find and map the location of Chestnut trees to be usedMake your beginning Biomass calculationsUse the biomass equations for chestnut trees from the Yale website

Identify all Chestnut trees in your area both with & without cankersMap the location of these trees and give each an identifierMeasure the diameter of each tree at chest height and record this data in a chart which will be attached to your mapBiomass Lab Protocol

To be able to prepare Hypovirulent Fungal Isolates to treat Chestnut Blight Cankers

Review of Hypovirulence PowerPoint Read over the protocolGather all materials necessaryPrepare the hypovirulent soup

To be able to treat Chestnut tree cankers located on trees in your test area

To prepare a data sheet in order compare canker growth before and after application of the soupTo prepare a data sheet for Biomass measurement comparisons

Follow the protocol for treating your chestnut tress with the soupTake before measurements of the cankers being treatedPhotograph the cankers and identify the photos as to which tree they belong

To be able to analyze biomass data obtained throughout the yearPresent data through the use of graphsDiscuss how soup application of cankers affected growth of the canker and growth of the tree in general

Measure Biomass of all treesMark in chartCompare growth of trees throughout the yearTake pictures of treated cankersEvaluate how the soup mixture workedCompare growth of canker infected trees to uninfected trees

Write a report whichAnalyzes the data you collectedDiscusses the change in biomassDiscusses biomass changes in canker infected trees to uninfected treesDiscusses how the soup mixture affected the treeDiscusses any unusual differences n biomass changes of your trees

Day 1*Answers to the questions are in the SR Soup lab Folder*Day 2-Chestnut Biomass equation is m=.0554dbh 2.4572 dbh=diameter at breast height Students will figure out the current biomass and take measurements at the end of the year to see the growth of the trees at the various sites. They can also compare canker infected trees to uninfected trees.*Map using either graph paper or a computer program, make sure trees with cankers are noted*Day 3-Contact Essie Burnworth 301-762-6715 to obtain the hypovirulent fungal strains in petri dishes or contact Judy Plaskowitz at South Carroll -410-751-3575 to see if she has some originals left*Day 4*Day 5-6*